Syt12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Syt12
Entrez Gene ID	171180
Full Name	synaptotagmin XII
General protein information	Preferred Names synaptotagmin XII Names synaptotagmin-12 sytXII
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
NM_134164.5	NP_598925.1	synaptotagmin-12

Pathways from BioSystems

Homologies

Homo sapiens (human)	SYT12	NP_001171351.1
	SYT12	XP_001117977.1
Mus musculus (house mouse)	Syt12	NP_598925.1
Rattus norvegicus (Norway rat)	Syt12	NP_620190.1
Pan troglodytes (chimpanzee)	SYT12	XP_001172576.2
Xenopus tropicalis (tropical clawed frog)	syt12	XP_002936546.1
Canis lupus familiaris (dog)	SYT12	XP_540824.3
Bos taurus (cattle)	SYT12	NP_001179819.1
Gallus gallus (chicken)	SYT12	NP_001186441.1
Danio rerio (zebrafish)	syt12	NP_001122205.1

Gene Ontology
Bibliography

Related articles in PubMed

Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.
Kaeser-Woo YJ, Younts TJ, Yang X, Zhou P, Wu D, Castillo PE, S?dhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience33(23)9769-80(2013 Jun)

Synaptotagmin-12, a synaptic vesicle phosphoprotein that modulates spontaneous neurotransmitter release.
Maximov A, Shin OH, Liu X, S?dhof TC
The Journal of cell biology176(1)113-24(2007 Jan)

Distinct developmental expression of synaptotagmin I and IX in the mouse brain.
Fukuda M
Neuroreport17(2)179-82(2006 Feb)

Mechanism of the SDS-resistant synaptotagmin clustering mediated by the cysteine cluster at the interface between the transmembrane and spacer domains.
Fukuda M, Kanno E, Ogata Y, Mikoshiba K
The Journal of biological chemistry276(43)40319-25(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Synaptotagmin-12 is essential for cyclic AMP-dependent protein kinase presynaptic long term potentiation at mossy-fiber synapses.
Title: Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.

The following Syt12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Syt12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10615	NM_134164.5 Latest version!	Mus musculus synaptotagmin XII (Syt12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Syt12 ( NM_134164.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598925.1 Mus musculus synaptotagmin XII (Syt12), mRNA.

Price & Availability↑

CloneID	OMu10615
Clone ID Related Accession (Same CDS sequence)	NM_134164.5
Accession Version	NM_134164.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	synaptotagmin-12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124347.4. On Oct 19, 2007 this sequence version replaced NM_134164.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK083139.1, SRR1660817.68639.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGTGG ACGTGACAGA ATATCATCTG AGCGTCATCA AGAGCCCCCC AGGCTGGGAG 
GTGGGTGTCT ATGCTGCCGG GGCCCTGGCT CTGCTGGGAA TTGCTGCTGT GAGTCTATGG 
AAGCTCTGGA CATCGGGGAG CTTCCCCAGC CCCTCCCCGT TCCCTAATTA CGACTACAGG 
TACCTGCAGC AGAAATATGG AGAGGCCTAT GTGGAGGCTA AGCTGAAGCG GGTCCCTCCT 
TGGAATGACC AGCGGACCAC TACTCGGGGA CCACCCAGCC GCAAAGGCAG CCTTAGTATA 
GAAGACACCT TTGAAAGCAT CAGTGAGCTG GGACCCCTGG AGCTGATGGG CCGGGAACTG 
GACTTAGCCC CCTATGGTAC TCTCCGAAAG TCACAGTCGG CTGACTCTCT GAATTCCATC 
TCCTCTGTGA GCAACACCTT TGGGCAGGAT TTCACCCTGG GCCAGGTGGA GGTGAGCATG 
GACTATGATG GAGCCTCCCA CACCCTCCAC GTGGCAGTAC TACAAGGCAA AGACCTCCTG 
GAGCGCGAGG AGGCCACCTT CGAGTCCTGC TTCATGCGTG TCAGTCTGCT GCCAGACGAA 
CAGATCGTGG GCATCTCCCG GATCCAGAGG AATGCCTATT CCATCTTCTT CGATGAGAAG 
TTCTCTGTCC CCCTGGATCC CACAGCCCTG GAGGAGAAGA GCCTTAGATT TTCTGTGTTT 
GGTATCGACG AGGATGAGCG GAATGTGAGC ACCGGCGTGG TGGAGCTGAA GCTGTCCGTT 
CTGGATCTCC CGCTGCAGCC CTTCAGTGGC TGGCTATACT TGCAGGACCA GAATAAGGCT 
GCTGACGCTG TGGGAGAGAT CCTGCTGTCC CTCAGTTACC TGCCCACAGC TGAGCGGCTG 
ACTGTGGTTG TGGTGAAAGC CAAGAATCTC ATCTGGACCA ATGAGAAGAG CACAGCAGAC 
CCCTTCGTCA AAGTATACCT GCTGCAGGAT GGGAGGAAAA TGAGCAAGAA GAAGACGGCT 
GTGAAGAGGG ATGACCCCAA CCCAGTGTTC AACGAAGCCA TGATCTTCTC AGTGCCAGCC 
ATTGTGCTAC AGGACCTGTC TCTCCGGGTG ACAGTGGCTG AGAGCAGCAG CGACGGCCGT 
GGGGACAACG TGGGCCATGT CATCATTGGG CCAGGGGTCA GTGGCATGGG CACCACACAT 
TGGAACCAGA TGCTGGCCAC ACTACGCAGG CCTGTGTCCA TGTGGCATCC AGTCCGGCGA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598925.1
CDS	233..1498
Translation	MAVDVTEYHLSVIKSPPGWEVGVYAAGALALLGIAAVSLWKLWTSGSFPSPSPFPNYDYRYLQQKYGEAYVEAKLKRVPPWNDQRTTTRGPPSRKGSLSIEDTFESISELGPLELMGRELDLAPYGTLRKSQSADSLNSISSVSNTFGQDFTLGQVEVSMDYDGASHTLHVAVLQGKDLLEREEATFESCFMRVSLLPDEQIVGISRIQRNAYSIFFDEKFSVPLDPTALEEKSLRFSVFGIDEDERNVSTGVVELKLSVLDLPLQPFSGWLYLQDQNKAADAVGEILLSLSYLPTAERLTVVVVKAKNLIWTNEKSTADPFVKVYLLQDGRKMSKKKTAVKRDDPNPVFNEAMIFSVPAIVLQDLSLRVTVAESSSDGRGDNVGHVIIGPGVSGMGTTHWNQMLATLRRPVSMWHPVRRN

Target ORF information:

RefSeq Version	NM_134164.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptotagmin XII (Syt12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134164.5

ORF Insert Sequence:

ATGGCCGTGG ACGTGACAGA ATATCATCTG AGCGTCATCA AGAGCCCCCC AGGCTGGGAG 
GTGGGTGTCT ATGCTGCCGG GGCCCTGGCT CTGCTGGGAA TTGCTGCTGT GAGTCTATGG 
AAGCTCTGGA CATCGGGGAG CTTCCCCAGC CCCTCCCCGT TCCCTAATTA CGACTACAGG 
TACCTGCAGC AGAAATATGG AGAGGCCTAT GTGGAGGCTA AGCTGAAGCG GGTCCCTCCT 
TGGAATGACC AGCGGACCAC TACTCGGGGA CCACCCAGCC GCAAAGGCAG CCTTAGTATA 
GAAGACACCT TTGAAAGCAT CAGTGAGCTG GGACCCCTGG AGCTGATGGG CCGGGAACTG 
GACTTAGCCC CCTATGGTAC TCTCCGAAAG TCACAGTCGG CTGACTCTCT GAATTCCATC 
TCCTCTGTGA GCAACACCTT TGGGCAGGAT TTCACCCTGG GCCAGGTGGA GGTGAGCATG 
GACTATGATG GAGCCTCCCA CACCCTCCAC GTGGCAGTAC TACAAGGCAA AGACCTCCTG 
GAGCGCGAGG AGGCCACCTT CGAGTCCTGC TTCATGCGTG TCAGTCTGCT GCCAGACGAA 
CAGATCGTGG GCATCTCCCG GATCCAGAGG AATGCCTATT CCATCTTCTT CGATGAGAAG 
TTCTCTGTCC CCCTGGATCC CACAGCCCTG GAGGAGAAGA GCCTTAGATT TTCTGTGTTT 
GGTATCGACG AGGATGAGCG GAATGTGAGC ACCGGCGTGG TGGAGCTGAA GCTGTCCGTT 
CTGGATCTCC CGCTGCAGCC CTTCAGTGGC TGGCTATACT TGCAGGACCA GAATAAGGCT 
GCTGACGCTG TGGGAGAGAT CCTGCTGTCC CTCAGTTACC TGCCCACAGC TGAGCGGCTG 
ACTGTGGTTG TGGTGAAAGC CAAGAATCTC ATCTGGACCA ATGAGAAGAG CACAGCAGAC 
CCCTTCGTCA AAGTATACCT GCTGCAGGAT GGGAGGAAAA TGAGCAAGAA GAAGACGGCT 
GTGAAGAGGG ATGACCCCAA CCCAGTGTTC AACGAAGCCA TGATCTTCTC AGTGCCAGCC 
ATTGTGCTAC AGGACCTGTC TCTCCGGGTG ACAGTGGCTG AGAGCAGCAG CGACGGCCGT 
GGGGACAACG TGGGCCATGT CATCATTGGG CCAGGGGTCA GTGGCATGGG CACCACACAT 
TGGAACCAGA TGCTGGCCAC ACTACGCAGG CCTGTGTCCA TGTGGCATCC AGTCCGGCGA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(23)9769-80(2013 Jun)
Kaeser-Woo YJ,Younts TJ,Yang X,Zhou P,Wu D,Castillo PE,S?dhof TC

Synaptotagmin-12, a synaptic vesicle phosphoprotein that modulates spontaneous neurotransmitter release.
The Journal of cell biology176(1)113-24(2007 Jan)
Maximov A,Shin OH,Liu X,S?dhof TC

Distinct developmental expression of synaptotagmin I and IX in the mouse brain.
Neuroreport17(2)179-82(2006 Feb)
Fukuda M

Mechanism of the SDS-resistant synaptotagmin clustering mediated by the cysteine cluster at the interface between the transmembrane and spacer domains.
The Journal of biological chemistry276(43)40319-25(2001 Oct)
Fukuda M,Kanno E,Ogata Y,Mikoshiba K