Lyst cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lyst
Entrez Gene ID	17101
Full Name	lysosomal trafficking regulator
Synonyms	D13Sfk13,beige,bg
General protein information	Preferred Names lysosomal trafficking regulator Names lysosomal-trafficking regulator CHS1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 A1\|13 5.28 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006516562.2	XP_006516625.1	lysosomal-trafficking regulator isoform X2
XM_006516562.1	XP_006516625.1	lysosomal-trafficking regulator isoform X2
XM_006516563.3	XP_006516626.1	lysosomal-trafficking regulator isoform X3
NM_010748.2	NP_034878.2	lysosomal-trafficking regulator
XM_006516561.2	XP_006516624.1	lysosomal-trafficking regulator isoform X1
XM_006516561.1	XP_006516624.1	lysosomal-trafficking regulator isoform X1
XM_006516563.4	XP_006516626.1	lysosomal-trafficking regulator isoform X3

Pathways from BioSystems

Homologies

	LOC718051	XP_002808311.1
Canis lupus familiaris (dog)	LYST	XP_005618856.1
Rattus norvegicus (Norway rat)	Lyst	NP_445970.1
Danio rerio (zebrafish)	lyst	XP_001919653.2
Bos taurus (cattle)	LYST	NP_776445.1
Gallus gallus (chicken)	LYST	XP_419558.4
Homo sapiens (human)	LYST	NP_000072.2
Mus musculus (house mouse)	Lyst	NP_034878.2
Xenopus tropicalis (tropical clawed frog)	lyst	XP_002935877.2
Pan troglodytes (chimpanzee)	LYST	XP_514288.2

Gene Ontology
Bibliography

Related articles in PubMed

Reversible immortalisation enables genetic correction of human muscle progenitors and engineering of next-generation human artificial chromosomes for Duchenne muscular dystrophy.
Benedetti S, Uno N, Hoshiya H, Ragazzi M, Ferrari G, Kazuki Y, Moyle LA, Tonlorenzi R, Lombardo A, Chaouch S, Mouly V, Moore M, Popplewell L, Kazuki K, Katoh M, Naldini L, Dickson G, Messina G, Oshimura M, Cossu G, Tedesco FS
EMBO molecular medicine(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lyst as a specific regulator of TLR3- and TLR4-mediated TRIF signaling pathways reveals how the regulation of the intracellular membrane trafficking network is functionally linked to specific TLR signaling pathways.
Title: Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor-mediated inflammatory responses.

results identify an association between oxidative damage to lipid membranes and the severity of Lyst-mutant phenotypes, revealing a new mechanism that contributes to pathophysiology involving LYST
Title: Elevated oxidative membrane damage associated with genetic modifiers of Lyst-mutant phenotypes.

These results demonstrated that mutation of the Lyst gene can produce ocular features of human XFS (exfoliation syndrome) and suggested that LYST or LYST-interacting genes may contribute to XFS.
Title: Lyst mutation in mice recapitulates iris defects of human exfoliation syndrome.

The upregulation of LYST/Beige in infected cells functions as a host innate response to limit parasite growth.
Title: Control of parasitophorous vacuole expansion by LYST/Beige restricts the intracellular growth of Leishmania amazonensis.

Apolipoprotein E (ApoE)-deficient beige mice have markedly reduced development of atherosclerotic lesions when fed a chow diet; expression of the beige mutation in all cell types involved in lesion development contributes to atheroprotection.
Title: Expression of the Lyst(beige) mutation is atheroprotective in chow-fed apolipoprotein E-deficient mice.

The following Lyst gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lyst cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu48327	XM_006516563.4 Latest version!	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu06780	NM_010748.2 Latest version!	Mus musculus lysosomal trafficking regulator (Lyst), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu48325	XM_006516561.2 Latest version!	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu48326	XM_006516562.2 Latest version!	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu48326	XM_006516562.1	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu48325	XM_006516561.1	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu48327	XM_006516563.3	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lyst ( XM_006516563.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516626.1 Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu48327
Clone ID Related Accession (Same CDS sequence)	XM_006516563.3 , XM_006516563.4
Accession Version	XM_006516563.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysosomal-trafficking regulator isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516563.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGAAGGTGAT AGACCAGAAG TTACAGAATC CATCAATCCT 
GGTGACAGAC TCATAGAAGA CGGCTGTATT CATTTGATTT CACTGGGGTC CAAAGCATTG 
ATGATCCAAG TGTGGGCTGA TCCCCACAGT GGCACTTTTA TCTTTCGTGT GTGCATGGAC 
TCAAATGATG ACACGAAAGC TGTCTCACTA GCACAGGTGG AATCACAGGA GAATATTTTC 
TTTCCAAGCA AATGGCAACA CTTAGTACTT ACCTATATTC AGCATCCTCA AGGGAAAAAG 
AATGTCCATG GGGAAATCTC CATATGGGTC TCTGGGCAGA GGAAGACTGA TGTCATCTTG 
GATTTTGTGC TCCCAAGAAA AACAAGCTTA TCATCAGACA GCAATAAAAC ATTTTGCATG 
ATTGGTCATT GCTTAACATC CCAAGAAGAG TCTCTGCAAT TAGCTGGAAA ATGGGACCTG 
GGGAACTTGC TCCTCTTCAA TGGAGCTAAA ATTGGCTCAC AAGAGGCCTT TTTCCTGTAT 
GCTTGTGGAC CCAACTACAC ATCCATCATG CCGTGTAAAT ATGGACAGCC AGTCATTGAC 
TACTCCAAAT ACATTAATAA AGACATTTTG AGATGTGATG AAATCAGAGA CCTTTTTATG 
ACCAAGAAAG AAGTGGATGT TGGTCTCTTA ATTGAAAGTC TTTCAGTTGT TTATACAACT 
TGCTGTCCTG CTCAGTACAC CATCTATGAA CCAGTGATTC GACTCAAGGG CCAAGTGAAA 
ACTCAGCCCT CTCAAAGACC CTTCAGCTCA AAGGAAGCCC AGAGCATCTT GCTAGAACCT 
TCTCAACTCA AAGGCCTCCA ACCTACGGAA TGTAAAGCCA TCCAGGGCAT TCTGCATGAG 
ATTGGTGGGG CTGGCACATT TGTTTTTCTC TTTGCTAGGG TTGTTGAACT TAGTAGCTGT 
GAAGAAACTC AAGCATTAGC ACTGCGGGTT ATACTGTCTT TAATTAAGTA CAGCCAACAG 
AGAACACAGG AACTGGAAAA TTGTAATGGA CTCTCTATGA TTCACCAAGT GTTGGTCAAA 
CAGAAATGCA TTGTTGGCTT TCACATTTTG AAGACCCTTC TTGAAGGTTG CTGCGGTGAA 
GAAGTTATCC ACGTCAGTGA GCATGGAGAG TTCAAGCTGG ATGTTGAGTC TCATGCTATA 
ATCCAAGATG TTAAGCTGCT GCAGGAACTG TTACTTGACT GGAAGATATG GAATAAGGCA 
GAGCAAGGTG TGTGGGAGAC TCTGCTAGCA GCTTTGGAAG TCCTCATCCG GGTAGAGCAC 
CACCAGCAGC AGTTTAATAT TAAGCAGTTG CTGAACGCCC ACGTGGTTCA CCACTTCCTA 
CTGACCTGTC AGGTTTTACA GGAACACAGA GAGGGGCAGC TTACATCTAT GCCCCGAGAA 
GTTTGTAGAT CATTTGTGAA AATCATTGCA GAAGTCCTTG GTTCTCCTCC AGACTTGGAA 
TTATTGACAG TTATTTTCAA TTTCCTGTTA GCTGTACACC CTCCTACTAA TACTTATGTT 
TGTCACAATC CCACAAACTT CTACTTCTCT TTGCACATAG ATGGCAAGAT CTTTCAGGAG 
AAAGTGCAGT CACTCGCGTA CCTGAGGCAT TCTAGCAGCG GAGGGCAAGC CTTTCCCAGC 
CCTGGATTCC TGGTAATAAG CCCATCTGCC TTTACTGCAG CTCCTCCTGA AGGAACCAGT 
TCTTCCAATA TTGTTCCACA GCGGATGGCT GCTCAGATGG TTCGATCTAG AAGTCTACCA 
GCATTTCCTA CTTATTTACC ACTAATACGA GCACAAAAAC TGGCTGCAAG TTTGGGTTTT 
AGTGTTGACA AGTTACAAAA TATTGCAGAT GCCAACCCAG AGAAACAGAA TCTTTTAGGA 
AGACCCTACG CACTGAAAAC AAGCAAAGAG GAAGCATTCA TCAGCAGCTG TGAGTCTGCA 
AAGACTGTTT GTGAAATGGA GGCTCTTCTT GGAGCCCACG CCTCTGCCAA TGGGGTTTCC 
AGAGGATCAC CGAGGTTCCC CAGGGCCAGA GTAGATCACA AAGATGTGGG AACAGAGCCC 
AGATCAGATG ATGACAGTCC TGGGGATGAG TCTTACCCAC GTCGGCCTGA CAACCTCAAG 
GGACTGGCCT CATTCCAGCG AAGCCAAAGC ACTGTCGCAA GCCTTGGGCT GGCGTTTCCC 
TCTCAGAATG GATCTGCAGT TGCTAGCAGG TGGCCAAGTC TTGTTGATAG GAATGCTGAT 
GACTGGGAGA ACTTTACCTT TTCTCCTGCT TATGAGGCAA GCTACAACCG AGCCACAAGC 
ACCCACAGTG TCATTGAAGA CTGTCTGATA CCTATCTGCT GTGGATTATA TGAACTCTTA 
AGTGGGGTTC TTCTTGTCCT GCCTGATGCT ATGCTTGAAG ATGTGATGGA CAGGATTATT 
CAAGCAGATA TTCTTCTAGT CCTTGTTAAC CACCCATCAC CTGCTATCCA GCAAGGAGTA 
ATTAAACTGT TACATGCATA CATTAATAGA GCATCAAAGG AGCAAAAGGA CAAGTTTCTG 
AAGAACCGTG GCTTTTCCTT ATTAGCCAAC CAGTTGTATC TTCATAGGGG AACTCAGGAG 
TTGTTGGAGT GCTTTGTTGA AATGTTCTTT GGTCGACCGA TTGGCCTGGA TGAAGAATTT 
GATCTGGAGG AAGTGAAGCA CATGGAACTG TTCCAGAAGT GGTCTGTCAT TCCCGTTCTC 
GGACTAATAG AGACCTCTCT CTATGACAAT GTCCTCTTGC ACAATGCTCT TTTACTTCTT 
CTGCAAGTTT TAAACTCTTG TTCCAAGGTA GCAGACATGC TACTGGACAA TGGTCTACTC 
TATGTATTAT GTAATACAGT AGCAGCCCTG AATGGATTAG AAAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516626.1
CDS	280..7710
Translation	MSTDSNSLAREFLIDVNQLCNAVVQRAEAREEEEEETHMATLGQYLVHGRGFLLLTKLNSIIDQALTCREELLTLLLSLLPLVWKIPVQEQQATDFNLPLSSDIILTKEKNSSLQKSTQGKLYLEGSAPSGQVSAKVNLFRKIRRQRKSTHRYSVRDARKTQLSTSDSEGNSDEKSTVVSKHRRLHALPRFLTQSPKEGHLVAKPDPSATKEQVLSDTMSVENSREVILRQDSNGDILSEPAALSILSNMNNSPFDLCHVLLSLLEKVCKFDIALNHNSSLALSVVPTLTEFLAGFGDCCNQSDTLEGQLVSAGWTEEPVALVQRMLFRTVLHLMSVDVSTAEAMPESLRKNLTELLRAALKIRACLEKQPEPFSPRQKKTLQEVQEGFVFSKYRHRALLLPELLEGVLQLLISCLQSAASNPFYFSQAMDLVQEFIQHQGFNLFETAVLQMEWLLSRDGVPSEAAEHLKALINSVIKIMSTVKKVKSEQLHHSMCTRKRHRRCEYSHFMQHHRDLSGLLVSAFKNQLSKSPFEETAEGDVQYPERCCCIAVCAHQCLRLLQQVSLSTTCVQILSGVHSVGICCCMDPKSVIVPLLHAFKLPALKAFQQHILNVLSKLLLDQLGGAELSPRIKKAACNICTVDSDQLAKLGETLQGTLCGAGPTSGLPSPSYRFQGILPSSGSEDLLWKWDALEAYQSFVFQEDRLHNIQIANHICNLIQKGNVVVQWKLYNYIFNPVLQRGVELVHHCQQLSIPSAQTHMCSQLKQYLPQEVLQIYLKTLPVLLKSRVIRDLFLSCNGVNQIIELNYLDGIRSHSLKAFETLIVSLGEQQKDAAVLDVDGLDIQQELPSLSVGPSLHKQQASSDSPCSLRKFYASLREPDPKKRKTIHQDVHINTINLFLCVAFLCVSKEADSDRESANESEDTSGYDSTASEPLSHMLPCLSLENVVLPSPECLHHAADIWSMCRWIYMLNSVFQKQFHRLGGFQVCHELIFMIIQKLFRSHTEDQGRRQGEMSRNENQELIRISYPELTLKGDVSSATAPDLGFLRKSADSVRGFQSQPVLPTSAEQIVATESVPGERKAFMSQQSETSLQSIRLLESLLAICLHSARACQQKMELELPSQGLSVENILCELREHLSQSKVAETELAKPLFDALLRVALGNHSADLGPGDAVTEKSHPSEEELLSQPGDFSEEAEDSQCCSLKLLGEEEGYEADSESNPEDVDTQDDGVELNPEAEGFSGSIVSNNLLENLTHGEIIYPEICMLGLNLLSASKAKLDVLAHVFESFLKIVRQKEKNISLLIQQGTVKILLGGFLNILTQTNSDFQACQRVLVDLLVSLMSSRTCSEDLTLLLRIFLEKSPCTEILLLGIHKIVESDFTMSPSQCLTFPFLHTPSLSNGVLSQKPPGILNSKALGLLRRARISRGKKEADRESFPYRLLSSWHIAPIHLPLLGQNCWPHLSEGFSVSLWFNVEYIHESESAAERGKRVKKRNKPSVLEDSSFEGAEGDRPEVTESINPGDRLIEDGCIHLISLGSKALMIQVWADPHSGTFIFRVCMDSNDDTKAVSLAQVESQENIFFPSKWQHLVLTYIQHPQGKKNVHGEISIWVSGQRKTDVILDFVLPRKTSLSSDSNKTFCMIGHCLTSQEESLQLAGKWDLGNLLLFNGAKIGSQEAFFLYACGPNYTSIMPCKYGQPVIDYSKYINKDILRCDEIRDLFMTKKEVDVGLLIESLSVVYTTCCPAQYTIYEPVIRLKGQVKTQPSQRPFSSKEAQSILLEPSQLKGLQPTECKAIQGILHEIGGAGTFVFLFARVVELSSCEETQALALRVILSLIKYSQQRTQELENCNGLSMIHQVLVKQKCIVGFHILKTLLEGCCGEEVIHVSEHGEFKLDVESHAIIQDVKLLQELLLDWKIWNKAEQGVWETLLAALEVLIRVEHHQQQFNIKQLLNAHVVHHFLLTCQVLQEHREGQLTSMPREVCRSFVKIIAEVLGSPPDLELLTVIFNFLLAVHPPTNTYVCHNPTNFYFSLHIDGKIFQEKVQSLAYLRHSSSGGQAFPSPGFLVISPSAFTAAPPEGTSSSNIVPQRMAAQMVRSRSLPAFPTYLPLIRAQKLAASLGFSVDKLQNIADANPEKQNLLGRPYALKTSKEEAFISSCESAKTVCEMEALLGAHASANGVSRGSPRFPRARVDHKDVGTEPRSDDDSPGDESYPRRPDNLKGLASFQRSQSTVASLGLAFPSQNGSAVASRWPSLVDRNADDWENFTFSPAYEASYNRATSTHSVIEDCLIPICCGLYELLSGVLLVLPDAMLEDVMDRIIQADILLVLVNHPSPAIQQGVIKLLHAYINRASKEQKDKFLKNRGFSLLANQLYLHRGTQELLECFVEMFFGRPIGLDEEFDLEEVKHMELFQKWSVIPVLGLIETSLYDNVLLHNALLLLLQVLNSCSKVADMLLDNGLLYVLCNTVAALNGLEKN

Target ORF information:

RefSeq Version	XM_006516563.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516563.4

ORF Insert Sequence:

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGAAGGTGAT AGACCAGAAG TTACAGAATC CATCAATCCT 
GGTGACAGAC TCATAGAAGA CGGCTGTATT CATTTGATTT CACTGGGGTC CAAAGCATTG 
ATGATCCAAG TGTGGGCTGA TCCCCACAGT GGCACTTTTA TCTTTCGTGT GTGCATGGAC 
TCAAATGATG ACACGAAAGC TGTCTCACTA GCACAGGTGG AATCACAGGA GAATATTTTC 
TTTCCAAGCA AATGGCAACA CTTAGTACTT ACCTATATTC AGCATCCTCA AGGGAAAAAG 
AATGTCCATG GGGAAATCTC CATATGGGTC TCTGGGCAGA GGAAGACTGA TGTCATCTTG 
GATTTTGTGC TCCCAAGAAA AACAAGCTTA TCATCAGACA GCAATAAAAC ATTTTGCATG 
ATTGGTCATT GCTTAACATC CCAAGAAGAG TCTCTGCAAT TAGCTGGAAA ATGGGACCTG 
GGGAACTTGC TCCTCTTCAA TGGAGCTAAA ATTGGCTCAC AAGAGGCCTT TTTCCTGTAT 
GCTTGTGGAC CCAACTACAC ATCCATCATG CCGTGTAAAT ATGGACAGCC AGTCATTGAC 
TACTCCAAAT ACATTAATAA AGACATTTTG AGATGTGATG AAATCAGAGA CCTTTTTATG 
ACCAAGAAAG AAGTGGATGT TGGTCTCTTA ATTGAAAGTC TTTCAGTTGT TTATACAACT 
TGCTGTCCTG CTCAGTACAC CATCTATGAA CCAGTGATTC GACTCAAGGG CCAAGTGAAA 
ACTCAGCCCT CTCAAAGACC CTTCAGCTCA AAGGAAGCCC AGAGCATCTT GCTAGAACCT 
TCTCAACTCA AAGGCCTCCA ACCTACGGAA TGTAAAGCCA TCCAGGGCAT TCTGCATGAG 
ATTGGTGGGG CTGGCACATT TGTTTTTCTC TTTGCTAGGG TTGTTGAACT TAGTAGCTGT 
GAAGAAACTC AAGCATTAGC ACTGCGGGTT ATACTGTCTT TAATTAAGTA CAGCCAACAG 
AGAACACAGG AACTGGAAAA TTGTAATGGA CTCTCTATGA TTCACCAAGT GTTGGTCAAA 
CAGAAATGCA TTGTTGGCTT TCACATTTTG AAGACCCTTC TTGAAGGTTG CTGCGGTGAA 
GAAGTTATCC ACGTCAGTGA GCATGGAGAG TTCAAGCTGG ATGTTGAGTC TCATGCTATA 
ATCCAAGATG TTAAGCTGCT GCAGGAACTG TTACTTGACT GGAAGATATG GAATAAGGCA 
GAGCAAGGTG TGTGGGAGAC TCTGCTAGCA GCTTTGGAAG TCCTCATCCG GGTAGAGCAC 
CACCAGCAGC AGTTTAATAT TAAGCAGTTG CTGAACGCCC ACGTGGTTCA CCACTTCCTA 
CTGACCTGTC AGGTTTTACA GGAACACAGA GAGGGGCAGC TTACATCTAT GCCCCGAGAA 
GTTTGTAGAT CATTTGTGAA AATCATTGCA GAAGTCCTTG GTTCTCCTCC AGACTTGGAA 
TTATTGACAG TTATTTTCAA TTTCCTGTTA GCTGTACACC CTCCTACTAA TACTTATGTT 
TGTCACAATC CCACAAACTT CTACTTCTCT TTGCACATAG ATGGCAAGAT CTTTCAGGAG 
AAAGTGCAGT CACTCGCGTA CCTGAGGCAT TCTAGCAGCG GAGGGCAAGC CTTTCCCAGC 
CCTGGATTCC TGGTAATAAG CCCATCTGCC TTTACTGCAG CTCCTCCTGA AGGAACCAGT 
TCTTCCAATA TTGTTCCACA GCGGATGGCT GCTCAGATGG TTCGATCTAG AAGTCTACCA 
GCATTTCCTA CTTATTTACC ACTAATACGA GCACAAAAAC TGGCTGCAAG TTTGGGTTTT 
AGTGTTGACA AGTTACAAAA TATTGCAGAT GCCAACCCAG AGAAACAGAA TCTTTTAGGA 
AGACCCTACG CACTGAAAAC AAGCAAAGAG GAAGCATTCA TCAGCAGCTG TGAGTCTGCA 
AAGACTGTTT GTGAAATGGA GGCTCTTCTT GGAGCCCACG CCTCTGCCAA TGGGGTTTCC 
AGAGGATCAC CGAGGTTCCC CAGGGCCAGA GTAGATCACA AAGATGTGGG AACAGAGCCC 
AGATCAGATG ATGACAGTCC TGGGGATGAG TCTTACCCAC GTCGGCCTGA CAACCTCAAG 
GGACTGGCCT CATTCCAGCG AAGCCAAAGC ACTGTCGCAA GCCTTGGGCT GGCGTTTCCC 
TCTCAGAATG GATCTGCAGT TGCTAGCAGG TGGCCAAGTC TTGTTGATAG GAATGCTGAT 
GACTGGGAGA ACTTTACCTT TTCTCCTGCT TATGAGGCAA GCTACAACCG AGCCACAAGC 
ACCCACAGTG TCATTGAAGA CTGTCTGATA CCTATCTGCT GTGGATTATA TGAACTCTTA 
AGTGGGGTTC TTCTTGTCCT GCCTGATGCT ATGCTTGAAG ATGTGATGGA CAGGATTATT 
CAAGCAGATA TTCTTCTAGT CCTTGTTAAC CACCCATCAC CTGCTATCCA GCAAGGAGTA 
ATTAAACTGT TACATGCATA CATTAATAGA GCATCAAAGG AGCAAAAGGA CAAGTTTCTG 
AAGAACCGTG GCTTTTCCTT ATTAGCCAAC CAGTTGTATC TTCATAGGGG AACTCAGGAG 
TTGTTGGAGT GCTTTGTTGA AATGTTCTTT GGTCGACCGA TTGGCCTGGA TGAAGAATTT 
GATCTGGAGG AAGTGAAGCA CATGGAACTG TTCCAGAAGT GGTCTGTCAT TCCCGTTCTC 
GGACTAATAG AGACCTCTCT CTATGACAAT GTCCTCTTGC ACAATGCTCT TTTACTTCTT 
CTGCAAGTTT TAAACTCTTG TTCCAAGGTA GCAGACATGC TACTGGACAA TGGTCTACTC 
TATGTATTAT GTAATACAGT AGCAGCCCTG AATGGATTAG AAAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lyst ( XM_006516563.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516626.1 Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X3, mRNA.

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CloneID	OMu48327
Clone ID Related Accession (Same CDS sequence)	XM_006516563.3 , XM_006516563.4
Accession Version	XM_006516563.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysosomal-trafficking regulator isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGAAGGTGAT AGACCAGAAG TTACAGAATC CATCAATCCT 
GGTGACAGAC TCATAGAAGA CGGCTGTATT CATTTGATTT CACTGGGGTC CAAAGCATTG 
ATGATCCAAG TGTGGGCTGA TCCCCACAGT GGCACTTTTA TCTTTCGTGT GTGCATGGAC 
TCAAATGATG ACACGAAAGC TGTCTCACTA GCACAGGTGG AATCACAGGA GAATATTTTC 
TTTCCAAGCA AATGGCAACA CTTAGTACTT ACCTATATTC AGCATCCTCA AGGGAAAAAG 
AATGTCCATG GGGAAATCTC CATATGGGTC TCTGGGCAGA GGAAGACTGA TGTCATCTTG 
GATTTTGTGC TCCCAAGAAA AACAAGCTTA TCATCAGACA GCAATAAAAC ATTTTGCATG 
ATTGGTCATT GCTTAACATC CCAAGAAGAG TCTCTGCAAT TAGCTGGAAA ATGGGACCTG 
GGGAACTTGC TCCTCTTCAA TGGAGCTAAA ATTGGCTCAC AAGAGGCCTT TTTCCTGTAT 
GCTTGTGGAC CCAACTACAC ATCCATCATG CCGTGTAAAT ATGGACAGCC AGTCATTGAC 
TACTCCAAAT ACATTAATAA AGACATTTTG AGATGTGATG AAATCAGAGA CCTTTTTATG 
ACCAAGAAAG AAGTGGATGT TGGTCTCTTA ATTGAAAGTC TTTCAGTTGT TTATACAACT 
TGCTGTCCTG CTCAGTACAC CATCTATGAA CCAGTGATTC GACTCAAGGG CCAAGTGAAA 
ACTCAGCCCT CTCAAAGACC CTTCAGCTCA AAGGAAGCCC AGAGCATCTT GCTAGAACCT 
TCTCAACTCA AAGGCCTCCA ACCTACGGAA TGTAAAGCCA TCCAGGGCAT TCTGCATGAG 
ATTGGTGGGG CTGGCACATT TGTTTTTCTC TTTGCTAGGG TTGTTGAACT TAGTAGCTGT 
GAAGAAACTC AAGCATTAGC ACTGCGGGTT ATACTGTCTT TAATTAAGTA CAGCCAACAG 
AGAACACAGG AACTGGAAAA TTGTAATGGA CTCTCTATGA TTCACCAAGT GTTGGTCAAA 
CAGAAATGCA TTGTTGGCTT TCACATTTTG AAGACCCTTC TTGAAGGTTG CTGCGGTGAA 
GAAGTTATCC ACGTCAGTGA GCATGGAGAG TTCAAGCTGG ATGTTGAGTC TCATGCTATA 
ATCCAAGATG TTAAGCTGCT GCAGGAACTG TTACTTGACT GGAAGATATG GAATAAGGCA 
GAGCAAGGTG TGTGGGAGAC TCTGCTAGCA GCTTTGGAAG TCCTCATCCG GGTAGAGCAC 
CACCAGCAGC AGTTTAATAT TAAGCAGTTG CTGAACGCCC ACGTGGTTCA CCACTTCCTA 
CTGACCTGTC AGGTTTTACA GGAACACAGA GAGGGGCAGC TTACATCTAT GCCCCGAGAA 
GTTTGTAGAT CATTTGTGAA AATCATTGCA GAAGTCCTTG GTTCTCCTCC AGACTTGGAA 
TTATTGACAG TTATTTTCAA TTTCCTGTTA GCTGTACACC CTCCTACTAA TACTTATGTT 
TGTCACAATC CCACAAACTT CTACTTCTCT TTGCACATAG ATGGCAAGAT CTTTCAGGAG 
AAAGTGCAGT CACTCGCGTA CCTGAGGCAT TCTAGCAGCG GAGGGCAAGC CTTTCCCAGC 
CCTGGATTCC TGGTAATAAG CCCATCTGCC TTTACTGCAG CTCCTCCTGA AGGAACCAGT 
TCTTCCAATA TTGTTCCACA GCGGATGGCT GCTCAGATGG TTCGATCTAG AAGTCTACCA 
GCATTTCCTA CTTATTTACC ACTAATACGA GCACAAAAAC TGGCTGCAAG TTTGGGTTTT 
AGTGTTGACA AGTTACAAAA TATTGCAGAT GCCAACCCAG AGAAACAGAA TCTTTTAGGA 
AGACCCTACG CACTGAAAAC AAGCAAAGAG GAAGCATTCA TCAGCAGCTG TGAGTCTGCA 
AAGACTGTTT GTGAAATGGA GGCTCTTCTT GGAGCCCACG CCTCTGCCAA TGGGGTTTCC 
AGAGGATCAC CGAGGTTCCC CAGGGCCAGA GTAGATCACA AAGATGTGGG AACAGAGCCC 
AGATCAGATG ATGACAGTCC TGGGGATGAG TCTTACCCAC GTCGGCCTGA CAACCTCAAG 
GGACTGGCCT CATTCCAGCG AAGCCAAAGC ACTGTCGCAA GCCTTGGGCT GGCGTTTCCC 
TCTCAGAATG GATCTGCAGT TGCTAGCAGG TGGCCAAGTC TTGTTGATAG GAATGCTGAT 
GACTGGGAGA ACTTTACCTT TTCTCCTGCT TATGAGGCAA GCTACAACCG AGCCACAAGC 
ACCCACAGTG TCATTGAAGA CTGTCTGATA CCTATCTGCT GTGGATTATA TGAACTCTTA 
AGTGGGGTTC TTCTTGTCCT GCCTGATGCT ATGCTTGAAG ATGTGATGGA CAGGATTATT 
CAAGCAGATA TTCTTCTAGT CCTTGTTAAC CACCCATCAC CTGCTATCCA GCAAGGAGTA 
ATTAAACTGT TACATGCATA CATTAATAGA GCATCAAAGG AGCAAAAGGA CAAGTTTCTG 
AAGAACCGTG GCTTTTCCTT ATTAGCCAAC CAGTTGTATC TTCATAGGGG AACTCAGGAG 
TTGTTGGAGT GCTTTGTTGA AATGTTCTTT GGTCGACCGA TTGGCCTGGA TGAAGAATTT 
GATCTGGAGG AAGTGAAGCA CATGGAACTG TTCCAGAAGT GGTCTGTCAT TCCCGTTCTC 
GGACTAATAG AGACCTCTCT CTATGACAAT GTCCTCTTGC ACAATGCTCT TTTACTTCTT 
CTGCAAGTTT TAAACTCTTG TTCCAAGGTA GCAGACATGC TACTGGACAA TGGTCTACTC 
TATGTATTAT GTAATACAGT AGCAGCCCTG AATGGATTAG AAAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516626.1
CDS	251..7681
Translation	MSTDSNSLAREFLIDVNQLCNAVVQRAEAREEEEEETHMATLGQYLVHGRGFLLLTKLNSIIDQALTCREELLTLLLSLLPLVWKIPVQEQQATDFNLPLSSDIILTKEKNSSLQKSTQGKLYLEGSAPSGQVSAKVNLFRKIRRQRKSTHRYSVRDARKTQLSTSDSEGNSDEKSTVVSKHRRLHALPRFLTQSPKEGHLVAKPDPSATKEQVLSDTMSVENSREVILRQDSNGDILSEPAALSILSNMNNSPFDLCHVLLSLLEKVCKFDIALNHNSSLALSVVPTLTEFLAGFGDCCNQSDTLEGQLVSAGWTEEPVALVQRMLFRTVLHLMSVDVSTAEAMPESLRKNLTELLRAALKIRACLEKQPEPFSPRQKKTLQEVQEGFVFSKYRHRALLLPELLEGVLQLLISCLQSAASNPFYFSQAMDLVQEFIQHQGFNLFETAVLQMEWLLSRDGVPSEAAEHLKALINSVIKIMSTVKKVKSEQLHHSMCTRKRHRRCEYSHFMQHHRDLSGLLVSAFKNQLSKSPFEETAEGDVQYPERCCCIAVCAHQCLRLLQQVSLSTTCVQILSGVHSVGICCCMDPKSVIVPLLHAFKLPALKAFQQHILNVLSKLLLDQLGGAELSPRIKKAACNICTVDSDQLAKLGETLQGTLCGAGPTSGLPSPSYRFQGILPSSGSEDLLWKWDALEAYQSFVFQEDRLHNIQIANHICNLIQKGNVVVQWKLYNYIFNPVLQRGVELVHHCQQLSIPSAQTHMCSQLKQYLPQEVLQIYLKTLPVLLKSRVIRDLFLSCNGVNQIIELNYLDGIRSHSLKAFETLIVSLGEQQKDAAVLDVDGLDIQQELPSLSVGPSLHKQQASSDSPCSLRKFYASLREPDPKKRKTIHQDVHINTINLFLCVAFLCVSKEADSDRESANESEDTSGYDSTASEPLSHMLPCLSLENVVLPSPECLHHAADIWSMCRWIYMLNSVFQKQFHRLGGFQVCHELIFMIIQKLFRSHTEDQGRRQGEMSRNENQELIRISYPELTLKGDVSSATAPDLGFLRKSADSVRGFQSQPVLPTSAEQIVATESVPGERKAFMSQQSETSLQSIRLLESLLAICLHSARACQQKMELELPSQGLSVENILCELREHLSQSKVAETELAKPLFDALLRVALGNHSADLGPGDAVTEKSHPSEEELLSQPGDFSEEAEDSQCCSLKLLGEEEGYEADSESNPEDVDTQDDGVELNPEAEGFSGSIVSNNLLENLTHGEIIYPEICMLGLNLLSASKAKLDVLAHVFESFLKIVRQKEKNISLLIQQGTVKILLGGFLNILTQTNSDFQACQRVLVDLLVSLMSSRTCSEDLTLLLRIFLEKSPCTEILLLGIHKIVESDFTMSPSQCLTFPFLHTPSLSNGVLSQKPPGILNSKALGLLRRARISRGKKEADRESFPYRLLSSWHIAPIHLPLLGQNCWPHLSEGFSVSLWFNVEYIHESESAAERGKRVKKRNKPSVLEDSSFEGAEGDRPEVTESINPGDRLIEDGCIHLISLGSKALMIQVWADPHSGTFIFRVCMDSNDDTKAVSLAQVESQENIFFPSKWQHLVLTYIQHPQGKKNVHGEISIWVSGQRKTDVILDFVLPRKTSLSSDSNKTFCMIGHCLTSQEESLQLAGKWDLGNLLLFNGAKIGSQEAFFLYACGPNYTSIMPCKYGQPVIDYSKYINKDILRCDEIRDLFMTKKEVDVGLLIESLSVVYTTCCPAQYTIYEPVIRLKGQVKTQPSQRPFSSKEAQSILLEPSQLKGLQPTECKAIQGILHEIGGAGTFVFLFARVVELSSCEETQALALRVILSLIKYSQQRTQELENCNGLSMIHQVLVKQKCIVGFHILKTLLEGCCGEEVIHVSEHGEFKLDVESHAIIQDVKLLQELLLDWKIWNKAEQGVWETLLAALEVLIRVEHHQQQFNIKQLLNAHVVHHFLLTCQVLQEHREGQLTSMPREVCRSFVKIIAEVLGSPPDLELLTVIFNFLLAVHPPTNTYVCHNPTNFYFSLHIDGKIFQEKVQSLAYLRHSSSGGQAFPSPGFLVISPSAFTAAPPEGTSSSNIVPQRMAAQMVRSRSLPAFPTYLPLIRAQKLAASLGFSVDKLQNIADANPEKQNLLGRPYALKTSKEEAFISSCESAKTVCEMEALLGAHASANGVSRGSPRFPRARVDHKDVGTEPRSDDDSPGDESYPRRPDNLKGLASFQRSQSTVASLGLAFPSQNGSAVASRWPSLVDRNADDWENFTFSPAYEASYNRATSTHSVIEDCLIPICCGLYELLSGVLLVLPDAMLEDVMDRIIQADILLVLVNHPSPAIQQGVIKLLHAYINRASKEQKDKFLKNRGFSLLANQLYLHRGTQELLECFVEMFFGRPIGLDEEFDLEEVKHMELFQKWSVIPVLGLIETSLYDNVLLHNALLLLLQVLNSCSKVADMLLDNGLLYVLCNTVAALNGLEKN

Target ORF information:

RefSeq Version	XM_006516563.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516563.3

ORF Insert Sequence:

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGAAGGTGAT AGACCAGAAG TTACAGAATC CATCAATCCT 
GGTGACAGAC TCATAGAAGA CGGCTGTATT CATTTGATTT CACTGGGGTC CAAAGCATTG 
ATGATCCAAG TGTGGGCTGA TCCCCACAGT GGCACTTTTA TCTTTCGTGT GTGCATGGAC 
TCAAATGATG ACACGAAAGC TGTCTCACTA GCACAGGTGG AATCACAGGA GAATATTTTC 
TTTCCAAGCA AATGGCAACA CTTAGTACTT ACCTATATTC AGCATCCTCA AGGGAAAAAG 
AATGTCCATG GGGAAATCTC CATATGGGTC TCTGGGCAGA GGAAGACTGA TGTCATCTTG 
GATTTTGTGC TCCCAAGAAA AACAAGCTTA TCATCAGACA GCAATAAAAC ATTTTGCATG 
ATTGGTCATT GCTTAACATC CCAAGAAGAG TCTCTGCAAT TAGCTGGAAA ATGGGACCTG 
GGGAACTTGC TCCTCTTCAA TGGAGCTAAA ATTGGCTCAC AAGAGGCCTT TTTCCTGTAT 
GCTTGTGGAC CCAACTACAC ATCCATCATG CCGTGTAAAT ATGGACAGCC AGTCATTGAC 
TACTCCAAAT ACATTAATAA AGACATTTTG AGATGTGATG AAATCAGAGA CCTTTTTATG 
ACCAAGAAAG AAGTGGATGT TGGTCTCTTA ATTGAAAGTC TTTCAGTTGT TTATACAACT 
TGCTGTCCTG CTCAGTACAC CATCTATGAA CCAGTGATTC GACTCAAGGG CCAAGTGAAA 
ACTCAGCCCT CTCAAAGACC CTTCAGCTCA AAGGAAGCCC AGAGCATCTT GCTAGAACCT 
TCTCAACTCA AAGGCCTCCA ACCTACGGAA TGTAAAGCCA TCCAGGGCAT TCTGCATGAG 
ATTGGTGGGG CTGGCACATT TGTTTTTCTC TTTGCTAGGG TTGTTGAACT TAGTAGCTGT 
GAAGAAACTC AAGCATTAGC ACTGCGGGTT ATACTGTCTT TAATTAAGTA CAGCCAACAG 
AGAACACAGG AACTGGAAAA TTGTAATGGA CTCTCTATGA TTCACCAAGT GTTGGTCAAA 
CAGAAATGCA TTGTTGGCTT TCACATTTTG AAGACCCTTC TTGAAGGTTG CTGCGGTGAA 
GAAGTTATCC ACGTCAGTGA GCATGGAGAG TTCAAGCTGG ATGTTGAGTC TCATGCTATA 
ATCCAAGATG TTAAGCTGCT GCAGGAACTG TTACTTGACT GGAAGATATG GAATAAGGCA 
GAGCAAGGTG TGTGGGAGAC TCTGCTAGCA GCTTTGGAAG TCCTCATCCG GGTAGAGCAC 
CACCAGCAGC AGTTTAATAT TAAGCAGTTG CTGAACGCCC ACGTGGTTCA CCACTTCCTA 
CTGACCTGTC AGGTTTTACA GGAACACAGA GAGGGGCAGC TTACATCTAT GCCCCGAGAA 
GTTTGTAGAT CATTTGTGAA AATCATTGCA GAAGTCCTTG GTTCTCCTCC AGACTTGGAA 
TTATTGACAG TTATTTTCAA TTTCCTGTTA GCTGTACACC CTCCTACTAA TACTTATGTT 
TGTCACAATC CCACAAACTT CTACTTCTCT TTGCACATAG ATGGCAAGAT CTTTCAGGAG 
AAAGTGCAGT CACTCGCGTA CCTGAGGCAT TCTAGCAGCG GAGGGCAAGC CTTTCCCAGC 
CCTGGATTCC TGGTAATAAG CCCATCTGCC TTTACTGCAG CTCCTCCTGA AGGAACCAGT 
TCTTCCAATA TTGTTCCACA GCGGATGGCT GCTCAGATGG TTCGATCTAG AAGTCTACCA 
GCATTTCCTA CTTATTTACC ACTAATACGA GCACAAAAAC TGGCTGCAAG TTTGGGTTTT 
AGTGTTGACA AGTTACAAAA TATTGCAGAT GCCAACCCAG AGAAACAGAA TCTTTTAGGA 
AGACCCTACG CACTGAAAAC AAGCAAAGAG GAAGCATTCA TCAGCAGCTG TGAGTCTGCA 
AAGACTGTTT GTGAAATGGA GGCTCTTCTT GGAGCCCACG CCTCTGCCAA TGGGGTTTCC 
AGAGGATCAC CGAGGTTCCC CAGGGCCAGA GTAGATCACA AAGATGTGGG AACAGAGCCC 
AGATCAGATG ATGACAGTCC TGGGGATGAG TCTTACCCAC GTCGGCCTGA CAACCTCAAG 
GGACTGGCCT CATTCCAGCG AAGCCAAAGC ACTGTCGCAA GCCTTGGGCT GGCGTTTCCC 
TCTCAGAATG GATCTGCAGT TGCTAGCAGG TGGCCAAGTC TTGTTGATAG GAATGCTGAT 
GACTGGGAGA ACTTTACCTT TTCTCCTGCT TATGAGGCAA GCTACAACCG AGCCACAAGC 
ACCCACAGTG TCATTGAAGA CTGTCTGATA CCTATCTGCT GTGGATTATA TGAACTCTTA 
AGTGGGGTTC TTCTTGTCCT GCCTGATGCT ATGCTTGAAG ATGTGATGGA CAGGATTATT 
CAAGCAGATA TTCTTCTAGT CCTTGTTAAC CACCCATCAC CTGCTATCCA GCAAGGAGTA 
ATTAAACTGT TACATGCATA CATTAATAGA GCATCAAAGG AGCAAAAGGA CAAGTTTCTG 
AAGAACCGTG GCTTTTCCTT ATTAGCCAAC CAGTTGTATC TTCATAGGGG AACTCAGGAG 
TTGTTGGAGT GCTTTGTTGA AATGTTCTTT GGTCGACCGA TTGGCCTGGA TGAAGAATTT 
GATCTGGAGG AAGTGAAGCA CATGGAACTG TTCCAGAAGT GGTCTGTCAT TCCCGTTCTC 
GGACTAATAG AGACCTCTCT CTATGACAAT GTCCTCTTGC ACAATGCTCT TTTACTTCTT 
CTGCAAGTTT TAAACTCTTG TTCCAAGGTA GCAGACATGC TACTGGACAA TGGTCTACTC 
TATGTATTAT GTAATACAGT AGCAGCCCTG AATGGATTAG AAAAGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lyst ( NM_010748.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034878.2 Mus musculus lysosomal trafficking regulator (Lyst), mRNA.

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CloneID	OMu06780
Clone ID Related Accession (Same CDS sequence)	NM_010748.2
Accession Version	NM_010748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Mus musculus(house mouse)
Product	lysosomal-trafficking regulator
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U70015.1 and CT010431.7. On Aug 11, 2006 this sequence version replaced NM_010748.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U70015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849387 [ECO:0000348] ##Evidence-Data-END##

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGAAGGTGAT AGACCAGAAG TTACAGAATC CATCAATCCT 
GGTGACAGAC TCATAGAAGA CGGCTGTATT CATTTGATTT CACTGGGGTC CAAAGCATTG 
ATGATCCAAG TGTGGGCTGA TCCCCACAGT GGCACTTTTA TCTTTCGTGT GTGCATGGAC 
TCAAATGATG ACACGAAAGC TGTCTCACTA GCACAGGTGG AATCACAGGA GAATATTTTC 
TTTCCAAGCA AATGGCAACA CTTAGTACTT ACCTATATTC AGCATCCTCA AGGGAAAAAG 
AATGTCCATG GGGAAATCTC CATATGGGTC TCTGGGCAGA GGAAGACTGA TGTCATCTTG 
GATTTTGTGC TCCCAAGAAA AACAAGCTTA TCATCAGACA GCAATAAAAC ATTTTGCATG 
ATTGGTCATT GCTTAACATC CCAAGAAGAG TCTCTGCAAT TAGCTGGAAA ATGGGACCTG 
GGGAACTTGC TCCTCTTCAA TGGAGCTAAA ATTGGCTCAC AAGAGGCCTT TTTCCTGTAT 
GCTTGTGGAC CCAACTACAC ATCCATCATG CCGTGTAAAT ATGGACAGCC AGTCATTGAC 
TACTCCAAAT ACATTAATAA AGACATTTTG AGATGTGATG AAATCAGAGA CCTTTTTATG 
ACCAAGAAAG AAGTGGATGT TGGTCTCTTA ATTGAAAGTC TTTCAGTTGT TTATACAACT 
TGCTGTCCTG CTCAGTACAC CATCTATGAA CCAGTGATTC GACTCAAGGG CCAAGTGAAA 
ACTCAGCCCT CTCAAAGACC CTTCAGCTCA AAGGAAGCCC AGAGCATCTT GCTAGAACCT 
TCTCAACTCA AAGGCCTCCA ACCTACGGAA TGTAAAGCCA TCCAGGGCAT TCTGCATGAG 
ATTGGTGGGG CTGGCACATT TGTTTTTCTC TTTGCTAGGG TTGTTGAACT TAGTAGCTGT 
GAAGAAACTC AAGCATTAGC ACTGCGGGTT ATACTGTCTT TAATTAAGTA CAGCCAACAG 
AGAACACAGG AACTGGAAAA TTGTAATGGA CTCTCTATGA TTCACCAAGT GTTGGTCAAA 
CAGAAATGCA TTGTTGGCTT TCACATTTTG AAGACCCTTC TTGAAGGTTG CTGCGGTGAA 
GAAGTTATCC ACGTCAGTGA GCATGGAGAG TTCAAGCTGG ATGTTGAGTC TCATGCTATA 
ATCCAAGATG TTAAGCTGCT GCAGGAACTG TTACTTGACT GGAAGATATG GAATAAGGCA 
GAGCAAGGTG TGTGGGAGAC TCTGCTAGCA GCTTTGGAAG TCCTCATCCG GGTAGAGCAC 
CACCAGCAGC AGTTTAATAT TAAGCAGTTG CTGAACGCCC ACGTGGTTCA CCACTTCCTA 
CTGACCTGTC AGGTTTTACA GGAACACAGA GAGGGGCAGC TTACATCTAT GCCCCGAGAA 
GTTTGTAGAT CATTTGTGAA AATCATTGCA GAAGTCCTTG GTTCTCCTCC AGACTTGGAA 
TTATTGACAG TTATTTTCAA TTTCCTGTTA GCTGTACACC CTCCTACTAA TACTTATGTT 
TGTCACAATC CCACAAACTT CTACTTCTCT TTGCACATAG ATGGCAAGAT CTTTCAGGAG 
AAAGTGCAGT CACTCGCGTA CCTGAGGCAT TCTAGCAGCG GAGGGCAAGC CTTTCCCAGC 
CCTGGATTCC TGGTAATAAG CCCATCTGCC TTTACTGCAG CTCCTCCTGA AGGAACCAGT 
TCTTCCAATA TTGTTCCACA GCGGATGGCT GCTCAGATGG TTCGATCTAG AAGTCTACCA 
GCATTTCCTA CTTATTTACC ACTAATACGA GCACAAAAAC TGGCTGCAAG TTTGGGTTTT 
AGTGTTGACA AGTTACAAAA TATTGCAGAT GCCAACCCAG AGAAACAGAA TCTTTTAGGA 
AGACCCTACG CACTGAAAAC AAGCAAAGAG GAAGCATTCA TCAGCAGCTG TGAGTCTGCA 
AAGACTGTTT GTGAAATGGA GGCTCTTCTT GGAGCCCACG CCTCTGCCAA TGGGGTTTCC 
AGAGGATCAC CGAGGTTCCC CAGGGCCAGA GTAGATCACA AAGATGTGGG AACAGAGCCC 
AGATCAGATG ATGACAGTCC TGGGGATGAG TCTTACCCAC GTCGGCCTGA CAACCTCAAG 
GGACTGGCCT CATTCCAGCG AAGCCAAAGC ACTGTCGCAA GCCTTGGGCT GGCGTTTCCC 
TCTCAGAATG GATCTGCAGT TGCTAGCAGG TGGCCAAGTC TTGTTGATAG GAATGCTGAT 
GACTGGGAGA ACTTTACCTT TTCTCCTGCT TATGAGGCAA GCTACAACCG AGCCACAAGC 
ACCCACAGTG TCATTGAAGA CTGTCTGATA CCTATCTGCT GTGGATTATA TGAACTCTTA 
AGTGGGGTTC TTCTTGTCCT GCCTGATGCT ATGCTTGAAG ATGTGATGGA CAGGATTATT 
CAAGCAGATA TTCTTCTAGT CCTTGTTAAC CACCCATCAC CTGCTATCCA GCAAGGAGTA 
ATTAAACTGT TACATGCATA CATTAATAGA GCATCAAAGG AGCAAAAGGA CAAGTTTCTG 
AAGAACCGTG GCTTTTCCTT ATTAGCCAAC CAGTTGTATC TTCATAGGGG AACTCAGGAG 
TTGTTGGAGT GCTTTGTTGA AATGTTCTTT GGTCGACCGA TTGGCCTGGA TGAAGAATTT 
GATCTGGAGG AAGTGAAGCA CATGGAACTG TTCCAGAAGT GGTCTGTCAT TCCCGTTCTC 
GGACTAATAG AGACCTCTCT CTATGACAAT GTCCTCTTGC ACAATGCTCT TTTACTTCTT 
CTGCAAGTTT TAAACTCTTG TTCCAAGGTA GCAGACATGC TACTGGACAA TGGTCTACTC 
TATGTATTAT GTAATACAGT AGCAGCCCTG AATGGATTAG AAAAGAACAT TCCTGTGAAC 
GAATACAAAT TGCTCGCATG TGATATACAG CAGCTTTTCA TAGCAGTTAC AATTCATGCT 
TGCAGTTCCT CAGGCACACA GTATTTTAGA GTGATTGAAG ACCTTATTGT ACTTCTTGGA 
TATCTTCATA ATAGCAAAAA CAAGAGGACA CAAAATATGG CTTTGGCCCT GCAGCTTAGA 
GTTCTCCAGG CTGCTTTGGA ATTTATAAGG AGCACAGCCA ATCATGACTC TGAAAGTCCA 
GTGCACTCGC CTTCTGCCCA CCGCCATTCA GTGCCTCCGA AGCGGAGAAG CATTGCTGGT 
TCTCGCAAAT TCCCTCTGGC TCAGACAGAG TCTCTGCTGA TGAAGATGCG CTCAGTGGCC 
AGCGATGAGC TACACTCTAT GATGCAGAGG AGGATGAGCC AAGAGCACCC CAGCCAGGCC 
TCGGAGGCAG AGCTCGCTCA GAGGCTGCAG AGGCTCACCA TCTTAGCTGT GAACAGGATT 
ATTTACCAAG AGTTGAATTC AGATATTATT GACATTTTGA GAACTCCAGA AAATACATCC 
CAAAGCAAGA CCTCAGTTTC TCAGACTGAA ATTTCTGAAG AAGACATGCA TCATGAGCAA 
CCTTCTGTAT ATAATCCATT TCAAAAAGAA ATGTTAACCT ATCTGTTGGA TGGCTTCAAA 
GTGTGTATTG GTTCAAGTAA AACTAGCGTT TCTAAGCAGC AGTGGACTAA AATCCTGGGG 
TCTTGTAAAG AAACCCTCCG AGACCAGCTT GGAAGATTGC TAGCGCATAT TTTGTCTCCA 
ACCCACACTG TACAAGAACG GAAGCAGATA CTTGAGATAG TTCATGAACC AGCTCACCAG 
GATATACTTC GTGACTGTCT TAGCCCCTCC CCACAACATG GAGCCAAGTT GGTTTTGTAT 
TTGTCAGAGT TGATACATAA TCATCAGGAT GAGTTAAGTG AAGAAGAAAT GGACACAGCA 
GAACTGCTTA TGAATGCTCT AAAGTTATGT GGCCACAAGT GCATCCCGCC CAGTGCCCCT 
TCCAAACCAG AGCTCATTAA GATCATCAGA GAGGAGCAAA AGAAGTATGA AAGTGAAGAG 
AGTGTGAGCA AAGGCTCATG GCAGAAAACG GTGAACAACA ACCAGCAAAG TCTCTTCCAG 
AGGCTCGATT TCAAATCCAA GGATATATCT AAAATCGCTG CAGACATCAC CCAGGCTGTA 
TCACTCTCCC AAGGCATTGA AAGGAAGAAG GTGATCCAGC ACATCAGAGG GATGTACAAA 
GTTGACCTGA GTGCCAGCAG GCACTGGCAG GAATGCATCC AGCAGCTGAC ACATGACAGA 
GCAGTCTGGT ATGACCCAAT CTACTATCCA ACTTCATGGC AGTTGGATCC AACAGAAGGG 
CCAAACCGAG AGAGGAGACG TTTGCAGAGA TGCTATCTAA CTATTCCCAA TAAGTACCTC 
CTGAGGGACA GACAGAAGTC AGAAGGTGTG CTCAGGCCCC CACTCTCTTA CCTTTTTGAA 
GATAAAACTC ATTCTTCCTT CTCCTCTACT GTCAAAGACA AAGCTGCAAG TGAATCCATC 
AGAGTGAATC GAAGATGTAT CAGTGTTGCA CCATCTAGAG AGACAGCTGG GGAATTGTTG 
TTAGGTAAAT GTGGGATGTA CTTTGTGGAA GACAATGCCT CTGACGCAGT TGAAAGCTCG 
AGCCTCCAAG GGGAGTTAGA GCCGGCATCA TTTTCTTGGA CATATGAGGA AATTAAAGAA 
GTTCACAGGC GCTGGTGGCA ACTAAGAGAT AATGCTGTAG AAATCTTTTT AACAAATGGC 
AGAACACTCC TATTAGCATT TGACAATAAC AAGGTTCGTG ATGACGTGTA CCAGAGCATC 
CTCACAAATA ACCTCCCAAA TCTTCTGGAG TACGGCAACA TCACCGCTCT GACAAACCTG 
TGGTATTCTG GACAAATTAC CAATTTTGAA TATTTGACTC ATTTAAACAA GCATGCGGGC 
CGGTCCTTCA ATGATCTCAT GCAGTACCCG GTGTTCCCCT TCATCCTTTC TGACTATGTT 
AGTGAGACTC TTGACCTCAA TGATCCATCT ATCTACAGAA ACCTATCTAA GCCTATAGCT 
GTGCAGTATA AAGAAAAAGA AGACCGTTAC GTTGACACAT ACAAGTACTT GGAGGAGGAG 
TATCGCAAGG GAGCTCGAGA GGATGACCCC ATGCCTCCTG TGCAACCCTA CCACTATGGC 
TCCCACTACT CCAACAGCGG CACCGTGCTC CACTTCCTGG TCAGGATGCC GCCTTTCACT 
AAAATGTTTC TAGCCTATCA AGATCAGAGT TTCGACATTC CAGACCGAAC ATTTCATTCT 
ACAAACACAA CTTGGCGCCT CTCCTCCTTT GAGTCCATGA CTGATGTGAA GGAGCTGATT 
CCAGAGTTTT TCTATCTTCC TGAGTTCTTA GTGAACCGTG AAGGCTTTGA CTTCGGTGTT 
CGTCAGAATG GAGAGCGGGT TAACCACGTC AATCTTCCTC CCTGGGCACG CAACGATCCT 
CGGCTGTTCA TCCTTATTCA CCGGCAAGCA CTAGAGTCTG ACCATGTGTC CCAGAACATC 
TGTCACTGGA TCGACTTAGT GTTTGGCTAC AAGCAAAAGG GGAAGGCGTC TGTTCAAGCC 
ATCAATGTCT TCCACCCTGC TACATATTTT GGAATGGATG TCTCTGCAGT TGAAGATCCA 
GTGCAGAGAC GGGCTTTAGA AACCATGATA AAAACCTACG GGCAGACCCC ACGTCAGTTG 
TTCCACACAG CCCATGCCAG CCGACCTGGA GCCAAGCTTA ACATCGAAGG AGAGCTTCCA 
GCAGCTGTTG GCTTGTTAGT CCAGTTCGCT TTCAGAGAGA CCCGAGAACC AGTCAAGGAA 
GTCACTCATC CGAGCCCTTT GTCATGGATA AAAGGCTTGA AGTGGGGGGA GTACGTAGGT 
TCCCCCAGTG CTCCAGTACC TGTGGTCTGC TTCAGCCAGC CCCATGGAGA AAGATTTGGT 
TCCCTGCAGG CACTGCCCAC CAGAGCCATC TGTGGTTTAT CACGAAACTT CTGTCTTCTG 
ATGACCTACA ACAAGGAGCA AGGTGTGAGA AGCATGAACA ACACCAATAT TCAGTGGTCT 
GCTATCCTAA GCTGGGGATA TGCTGACAAC ATCTTACGGT TGAAAAGTAA GCAGAGTGAG 
CCACCAATCA ACTTCATTCA GAGTTCACAG CAGCACCAGG TAACCAGTTG TGCCTGGGTG 
CCTGACAGTT GTCAGCTCTT CACTGGGAGC AAGTGTGGTG TCATCACAGC CTATACCAAC 
AGGCTCACCA GCAGCACGCC CTCAGAAATT GAAATGGAGA GTCAGATGCA TCTCTATGGA 
CACACAGAGG AGATCACCGG CTTATGTGTC TGCAAGCCGT ACAGCGTGAT GATAAGCGTG 
AGCAGAGACG GGACCTGCAT AGTATGGGAC CTGAACAGGC TGTGCTATGT ACAAAGTTTG 
GCTGGACACA AAAGCCCTGT GACGGCTGTC TCTGCCAGTG AAACGTCAGG TGACATTGCT 
ACTGTGTGTG ACTCAGCTGG CGGGGGCAGT GACCTGAGAC TCTGGACCGT GAATGGGGAC 
CTCGTTGGAC ATGTCCACTG CAGAGAGATC ATTTGTTCTG TAGCTTTCTC CAACCAGCCT 
GAGGGAGTCT CCATCAACGT CATTGCTGGG GGATTAGAAA ATGGCATTGT AAGGCTATGG 
AGCACATGGG ACTTGAAGCC TGTGAGAGAG ATTACATTTC CCAAATCAAA TAAGCCCATC 
ATAAGCCTGA CATTCTCCTG TGATGGCCAC CATTTGTACA CTGCCAACAG TGAGGGGACA 
GTGATCGCAT GGTGCCGGAA GGACCAGCAG CGTGTGAAGC TGCCCATGTT CTACTCTTTC 
CTCAGCAGCT ACGCAGCTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034878.2
CDS	182..11545
Translation	MSTDSNSLAREFLIDVNQLCNAVVQRAEAREEEEEETHMATLGQYLVHGRGFLLLTKLNSIIDQALTCREELLTLLLSLLPLVWKIPVQEQQATDFNLPLSSDIILTKEKNSSLQKSTQGKLYLEGSAPSGQVSAKVNLFRKIRRQRKSTHRYSVRDARKTQLSTSDSEGNSDEKSTVVSKHRRLHALPRFLTQSPKEGHLVAKPDPSATKEQVLSDTMSVENSREVILRQDSNGDILSEPAALSILSNMNNSPFDLCHVLLSLLEKVCKFDIALNHNSSLALSVVPTLTEFLAGFGDCCNQSDTLEGQLVSAGWTEEPVALVQRMLFRTVLHLMSVDVSTAEAMPESLRKNLTELLRAALKIRACLEKQPEPFSPRQKKTLQEVQEGFVFSKYRHRALLLPELLEGVLQLLISCLQSAASNPFYFSQAMDLVQEFIQHQGFNLFETAVLQMEWLLSRDGVPSEAAEHLKALINSVIKIMSTVKKVKSEQLHHSMCTRKRHRRCEYSHFMQHHRDLSGLLVSAFKNQLSKSPFEETAEGDVQYPERCCCIAVCAHQCLRLLQQVSLSTTCVQILSGVHSVGICCCMDPKSVIVPLLHAFKLPALKAFQQHILNVLSKLLLDQLGGAELSPRIKKAACNICTVDSDQLAKLGETLQGTLCGAGPTSGLPSPSYRFQGILPSSGSEDLLWKWDALEAYQSFVFQEDRLHNIQIANHICNLIQKGNVVVQWKLYNYIFNPVLQRGVELVHHCQQLSIPSAQTHMCSQLKQYLPQEVLQIYLKTLPVLLKSRVIRDLFLSCNGVNQIIELNYLDGIRSHSLKAFETLIVSLGEQQKDAAVLDVDGLDIQQELPSLSVGPSLHKQQASSDSPCSLRKFYASLREPDPKKRKTIHQDVHINTINLFLCVAFLCVSKEADSDRESANESEDTSGYDSTASEPLSHMLPCLSLENVVLPSPECLHHAADIWSMCRWIYMLNSVFQKQFHRLGGFQVCHELIFMIIQKLFRSHTEDQGRRQGEMSRNENQELIRISYPELTLKGDVSSATAPDLGFLRKSADSVRGFQSQPVLPTSAEQIVATESVPGERKAFMSQQSETSLQSIRLLESLLAICLHSARACQQKMELELPSQGLSVENILCELREHLSQSKVAETELAKPLFDALLRVALGNHSADLGPGDAVTEKSHPSEEELLSQPGDFSEEAEDSQCCSLKLLGEEEGYEADSESNPEDVDTQDDGVELNPEAEGFSGSIVSNNLLENLTHGEIIYPEICMLGLNLLSASKAKLDVLAHVFESFLKIVRQKEKNISLLIQQGTVKILLGGFLNILTQTNSDFQACQRVLVDLLVSLMSSRTCSEDLTLLLRIFLEKSPCTEILLLGIHKIVESDFTMSPSQCLTFPFLHTPSLSNGVLSQKPPGILNSKALGLLRRARISRGKKEADRESFPYRLLSSWHIAPIHLPLLGQNCWPHLSEGFSVSLWFNVEYIHESESAAERGKRVKKRNKPSVLEDSSFEGAEGDRPEVTESINPGDRLIEDGCIHLISLGSKALMIQVWADPHSGTFIFRVCMDSNDDTKAVSLAQVESQENIFFPSKWQHLVLTYIQHPQGKKNVHGEISIWVSGQRKTDVILDFVLPRKTSLSSDSNKTFCMIGHCLTSQEESLQLAGKWDLGNLLLFNGAKIGSQEAFFLYACGPNYTSIMPCKYGQPVIDYSKYINKDILRCDEIRDLFMTKKEVDVGLLIESLSVVYTTCCPAQYTIYEPVIRLKGQVKTQPSQRPFSSKEAQSILLEPSQLKGLQPTECKAIQGILHEIGGAGTFVFLFARVVELSSCEETQALALRVILSLIKYSQQRTQELENCNGLSMIHQVLVKQKCIVGFHILKTLLEGCCGEEVIHVSEHGEFKLDVESHAIIQDVKLLQELLLDWKIWNKAEQGVWETLLAALEVLIRVEHHQQQFNIKQLLNAHVVHHFLLTCQVLQEHREGQLTSMPREVCRSFVKIIAEVLGSPPDLELLTVIFNFLLAVHPPTNTYVCHNPTNFYFSLHIDGKIFQEKVQSLAYLRHSSSGGQAFPSPGFLVISPSAFTAAPPEGTSSSNIVPQRMAAQMVRSRSLPAFPTYLPLIRAQKLAASLGFSVDKLQNIADANPEKQNLLGRPYALKTSKEEAFISSCESAKTVCEMEALLGAHASANGVSRGSPRFPRARVDHKDVGTEPRSDDDSPGDESYPRRPDNLKGLASFQRSQSTVASLGLAFPSQNGSAVASRWPSLVDRNADDWENFTFSPAYEASYNRATSTHSVIEDCLIPICCGLYELLSGVLLVLPDAMLEDVMDRIIQADILLVLVNHPSPAIQQGVIKLLHAYINRASKEQKDKFLKNRGFSLLANQLYLHRGTQELLECFVEMFFGRPIGLDEEFDLEEVKHMELFQKWSVIPVLGLIETSLYDNVLLHNALLLLLQVLNSCSKVADMLLDNGLLYVLCNTVAALNGLEKNIPVNEYKLLACDIQQLFIAVTIHACSSSGTQYFRVIEDLIVLLGYLHNSKNKRTQNMALALQLRVLQAALEFIRSTANHDSESPVHSPSAHRHSVPPKRRSIAGSRKFPLAQTESLLMKMRSVASDELHSMMQRRMSQEHPSQASEAELAQRLQRLTILAVNRIIYQELNSDIIDILRTPENTSQSKTSVSQTEISEEDMHHEQPSVYNPFQKEMLTYLLDGFKVCIGSSKTSVSKQQWTKILGSCKETLRDQLGRLLAHILSPTHTVQERKQILEIVHEPAHQDILRDCLSPSPQHGAKLVLYLSELIHNHQDELSEEEMDTAELLMNALKLCGHKCIPPSAPSKPELIKIIREEQKKYESEESVSKGSWQKTVNNNQQSLFQRLDFKSKDISKIAADITQAVSLSQGIERKKVIQHIRGMYKVDLSASRHWQECIQQLTHDRAVWYDPIYYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLRDRQKSEGVLRPPLSYLFEDKTHSSFSSTVKDKAASESIRVNRRCISVAPSRETAGELLLGKCGMYFVEDNASDAVESSSLQGELEPASFSWTYEEIKEVHRRWWQLRDNAVEIFLTNGRTLLLAFDNNKVRDDVYQSILTNNLPNLLEYGNITALTNLWYSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILSDYVSETLDLNDPSIYRNLSKPIAVQYKEKEDRYVDTYKYLEEEYRKGAREDDPMPPVQPYHYGSHYSNSGTVLHFLVRMPPFTKMFLAYQDQSFDIPDRTFHSTNTTWRLSSFESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNGERVNHVNLPPWARNDPRLFILIHRQALESDHVSQNICHWIDLVFGYKQKGKASVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFHTAHASRPGAKLNIEGELPAAVGLLVQFAFRETREPVKEVTHPSPLSWIKGLKWGEYVGSPSAPVPVVCFSQPHGERFGSLQALPTRAICGLSRNFCLLMTYNKEQGVRSMNNTNIQWSAILSWGYADNILRLKSKQSEPPINFIQSSQQHQVTSCAWVPDSCQLFTGSKCGVITAYTNRLTSSTPSEIEMESQMHLYGHTEEITGLCVCKPYSVMISVSRDGTCIVWDLNRLCYVQSLAGHKSPVTAVSASETSGDIATVCDSAGGGSDLRLWTVNGDLVGHVHCREIICSVAFSNQPEGVSINVIAGGLENGIVRLWSTWDLKPVREITFPKSNKPIISLTFSCDGHHLYTANSEGTVIAWCRKDQQRVKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	NM_010748.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysosomal trafficking regulator (Lyst), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010748.2

ORF Insert Sequence:

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGAAGGTGAT AGACCAGAAG TTACAGAATC CATCAATCCT 
GGTGACAGAC TCATAGAAGA CGGCTGTATT CATTTGATTT CACTGGGGTC CAAAGCATTG 
ATGATCCAAG TGTGGGCTGA TCCCCACAGT GGCACTTTTA TCTTTCGTGT GTGCATGGAC 
TCAAATGATG ACACGAAAGC TGTCTCACTA GCACAGGTGG AATCACAGGA GAATATTTTC 
TTTCCAAGCA AATGGCAACA CTTAGTACTT ACCTATATTC AGCATCCTCA AGGGAAAAAG 
AATGTCCATG GGGAAATCTC CATATGGGTC TCTGGGCAGA GGAAGACTGA TGTCATCTTG 
GATTTTGTGC TCCCAAGAAA AACAAGCTTA TCATCAGACA GCAATAAAAC ATTTTGCATG 
ATTGGTCATT GCTTAACATC CCAAGAAGAG TCTCTGCAAT TAGCTGGAAA ATGGGACCTG 
GGGAACTTGC TCCTCTTCAA TGGAGCTAAA ATTGGCTCAC AAGAGGCCTT TTTCCTGTAT 
GCTTGTGGAC CCAACTACAC ATCCATCATG CCGTGTAAAT ATGGACAGCC AGTCATTGAC 
TACTCCAAAT ACATTAATAA AGACATTTTG AGATGTGATG AAATCAGAGA CCTTTTTATG 
ACCAAGAAAG AAGTGGATGT TGGTCTCTTA ATTGAAAGTC TTTCAGTTGT TTATACAACT 
TGCTGTCCTG CTCAGTACAC CATCTATGAA CCAGTGATTC GACTCAAGGG CCAAGTGAAA 
ACTCAGCCCT CTCAAAGACC CTTCAGCTCA AAGGAAGCCC AGAGCATCTT GCTAGAACCT 
TCTCAACTCA AAGGCCTCCA ACCTACGGAA TGTAAAGCCA TCCAGGGCAT TCTGCATGAG 
ATTGGTGGGG CTGGCACATT TGTTTTTCTC TTTGCTAGGG TTGTTGAACT TAGTAGCTGT 
GAAGAAACTC AAGCATTAGC ACTGCGGGTT ATACTGTCTT TAATTAAGTA CAGCCAACAG 
AGAACACAGG AACTGGAAAA TTGTAATGGA CTCTCTATGA TTCACCAAGT GTTGGTCAAA 
CAGAAATGCA TTGTTGGCTT TCACATTTTG AAGACCCTTC TTGAAGGTTG CTGCGGTGAA 
GAAGTTATCC ACGTCAGTGA GCATGGAGAG TTCAAGCTGG ATGTTGAGTC TCATGCTATA 
ATCCAAGATG TTAAGCTGCT GCAGGAACTG TTACTTGACT GGAAGATATG GAATAAGGCA 
GAGCAAGGTG TGTGGGAGAC TCTGCTAGCA GCTTTGGAAG TCCTCATCCG GGTAGAGCAC 
CACCAGCAGC AGTTTAATAT TAAGCAGTTG CTGAACGCCC ACGTGGTTCA CCACTTCCTA 
CTGACCTGTC AGGTTTTACA GGAACACAGA GAGGGGCAGC TTACATCTAT GCCCCGAGAA 
GTTTGTAGAT CATTTGTGAA AATCATTGCA GAAGTCCTTG GTTCTCCTCC AGACTTGGAA 
TTATTGACAG TTATTTTCAA TTTCCTGTTA GCTGTACACC CTCCTACTAA TACTTATGTT 
TGTCACAATC CCACAAACTT CTACTTCTCT TTGCACATAG ATGGCAAGAT CTTTCAGGAG 
AAAGTGCAGT CACTCGCGTA CCTGAGGCAT TCTAGCAGCG GAGGGCAAGC CTTTCCCAGC 
CCTGGATTCC TGGTAATAAG CCCATCTGCC TTTACTGCAG CTCCTCCTGA AGGAACCAGT 
TCTTCCAATA TTGTTCCACA GCGGATGGCT GCTCAGATGG TTCGATCTAG AAGTCTACCA 
GCATTTCCTA CTTATTTACC ACTAATACGA GCACAAAAAC TGGCTGCAAG TTTGGGTTTT 
AGTGTTGACA AGTTACAAAA TATTGCAGAT GCCAACCCAG AGAAACAGAA TCTTTTAGGA 
AGACCCTACG CACTGAAAAC AAGCAAAGAG GAAGCATTCA TCAGCAGCTG TGAGTCTGCA 
AAGACTGTTT GTGAAATGGA GGCTCTTCTT GGAGCCCACG CCTCTGCCAA TGGGGTTTCC 
AGAGGATCAC CGAGGTTCCC CAGGGCCAGA GTAGATCACA AAGATGTGGG AACAGAGCCC 
AGATCAGATG ATGACAGTCC TGGGGATGAG TCTTACCCAC GTCGGCCTGA CAACCTCAAG 
GGACTGGCCT CATTCCAGCG AAGCCAAAGC ACTGTCGCAA GCCTTGGGCT GGCGTTTCCC 
TCTCAGAATG GATCTGCAGT TGCTAGCAGG TGGCCAAGTC TTGTTGATAG GAATGCTGAT 
GACTGGGAGA ACTTTACCTT TTCTCCTGCT TATGAGGCAA GCTACAACCG AGCCACAAGC 
ACCCACAGTG TCATTGAAGA CTGTCTGATA CCTATCTGCT GTGGATTATA TGAACTCTTA 
AGTGGGGTTC TTCTTGTCCT GCCTGATGCT ATGCTTGAAG ATGTGATGGA CAGGATTATT 
CAAGCAGATA TTCTTCTAGT CCTTGTTAAC CACCCATCAC CTGCTATCCA GCAAGGAGTA 
ATTAAACTGT TACATGCATA CATTAATAGA GCATCAAAGG AGCAAAAGGA CAAGTTTCTG 
AAGAACCGTG GCTTTTCCTT ATTAGCCAAC CAGTTGTATC TTCATAGGGG AACTCAGGAG 
TTGTTGGAGT GCTTTGTTGA AATGTTCTTT GGTCGACCGA TTGGCCTGGA TGAAGAATTT 
GATCTGGAGG AAGTGAAGCA CATGGAACTG TTCCAGAAGT GGTCTGTCAT TCCCGTTCTC 
GGACTAATAG AGACCTCTCT CTATGACAAT GTCCTCTTGC ACAATGCTCT TTTACTTCTT 
CTGCAAGTTT TAAACTCTTG TTCCAAGGTA GCAGACATGC TACTGGACAA TGGTCTACTC 
TATGTATTAT GTAATACAGT AGCAGCCCTG AATGGATTAG AAAAGAACAT TCCTGTGAAC 
GAATACAAAT TGCTCGCATG TGATATACAG CAGCTTTTCA TAGCAGTTAC AATTCATGCT 
TGCAGTTCCT CAGGCACACA GTATTTTAGA GTGATTGAAG ACCTTATTGT ACTTCTTGGA 
TATCTTCATA ATAGCAAAAA CAAGAGGACA CAAAATATGG CTTTGGCCCT GCAGCTTAGA 
GTTCTCCAGG CTGCTTTGGA ATTTATAAGG AGCACAGCCA ATCATGACTC TGAAAGTCCA 
GTGCACTCGC CTTCTGCCCA CCGCCATTCA GTGCCTCCGA AGCGGAGAAG CATTGCTGGT 
TCTCGCAAAT TCCCTCTGGC TCAGACAGAG TCTCTGCTGA TGAAGATGCG CTCAGTGGCC 
AGCGATGAGC TACACTCTAT GATGCAGAGG AGGATGAGCC AAGAGCACCC CAGCCAGGCC 
TCGGAGGCAG AGCTCGCTCA GAGGCTGCAG AGGCTCACCA TCTTAGCTGT GAACAGGATT 
ATTTACCAAG AGTTGAATTC AGATATTATT GACATTTTGA GAACTCCAGA AAATACATCC 
CAAAGCAAGA CCTCAGTTTC TCAGACTGAA ATTTCTGAAG AAGACATGCA TCATGAGCAA 
CCTTCTGTAT ATAATCCATT TCAAAAAGAA ATGTTAACCT ATCTGTTGGA TGGCTTCAAA 
GTGTGTATTG GTTCAAGTAA AACTAGCGTT TCTAAGCAGC AGTGGACTAA AATCCTGGGG 
TCTTGTAAAG AAACCCTCCG AGACCAGCTT GGAAGATTGC TAGCGCATAT TTTGTCTCCA 
ACCCACACTG TACAAGAACG GAAGCAGATA CTTGAGATAG TTCATGAACC AGCTCACCAG 
GATATACTTC GTGACTGTCT TAGCCCCTCC CCACAACATG GAGCCAAGTT GGTTTTGTAT 
TTGTCAGAGT TGATACATAA TCATCAGGAT GAGTTAAGTG AAGAAGAAAT GGACACAGCA 
GAACTGCTTA TGAATGCTCT AAAGTTATGT GGCCACAAGT GCATCCCGCC CAGTGCCCCT 
TCCAAACCAG AGCTCATTAA GATCATCAGA GAGGAGCAAA AGAAGTATGA AAGTGAAGAG 
AGTGTGAGCA AAGGCTCATG GCAGAAAACG GTGAACAACA ACCAGCAAAG TCTCTTCCAG 
AGGCTCGATT TCAAATCCAA GGATATATCT AAAATCGCTG CAGACATCAC CCAGGCTGTA 
TCACTCTCCC AAGGCATTGA AAGGAAGAAG GTGATCCAGC ACATCAGAGG GATGTACAAA 
GTTGACCTGA GTGCCAGCAG GCACTGGCAG GAATGCATCC AGCAGCTGAC ACATGACAGA 
GCAGTCTGGT ATGACCCAAT CTACTATCCA ACTTCATGGC AGTTGGATCC AACAGAAGGG 
CCAAACCGAG AGAGGAGACG TTTGCAGAGA TGCTATCTAA CTATTCCCAA TAAGTACCTC 
CTGAGGGACA GACAGAAGTC AGAAGGTGTG CTCAGGCCCC CACTCTCTTA CCTTTTTGAA 
GATAAAACTC ATTCTTCCTT CTCCTCTACT GTCAAAGACA AAGCTGCAAG TGAATCCATC 
AGAGTGAATC GAAGATGTAT CAGTGTTGCA CCATCTAGAG AGACAGCTGG GGAATTGTTG 
TTAGGTAAAT GTGGGATGTA CTTTGTGGAA GACAATGCCT CTGACGCAGT TGAAAGCTCG 
AGCCTCCAAG GGGAGTTAGA GCCGGCATCA TTTTCTTGGA CATATGAGGA AATTAAAGAA 
GTTCACAGGC GCTGGTGGCA ACTAAGAGAT AATGCTGTAG AAATCTTTTT AACAAATGGC 
AGAACACTCC TATTAGCATT TGACAATAAC AAGGTTCGTG ATGACGTGTA CCAGAGCATC 
CTCACAAATA ACCTCCCAAA TCTTCTGGAG TACGGCAACA TCACCGCTCT GACAAACCTG 
TGGTATTCTG GACAAATTAC CAATTTTGAA TATTTGACTC ATTTAAACAA GCATGCGGGC 
CGGTCCTTCA ATGATCTCAT GCAGTACCCG GTGTTCCCCT TCATCCTTTC TGACTATGTT 
AGTGAGACTC TTGACCTCAA TGATCCATCT ATCTACAGAA ACCTATCTAA GCCTATAGCT 
GTGCAGTATA AAGAAAAAGA AGACCGTTAC GTTGACACAT ACAAGTACTT GGAGGAGGAG 
TATCGCAAGG GAGCTCGAGA GGATGACCCC ATGCCTCCTG TGCAACCCTA CCACTATGGC 
TCCCACTACT CCAACAGCGG CACCGTGCTC CACTTCCTGG TCAGGATGCC GCCTTTCACT 
AAAATGTTTC TAGCCTATCA AGATCAGAGT TTCGACATTC CAGACCGAAC ATTTCATTCT 
ACAAACACAA CTTGGCGCCT CTCCTCCTTT GAGTCCATGA CTGATGTGAA GGAGCTGATT 
CCAGAGTTTT TCTATCTTCC TGAGTTCTTA GTGAACCGTG AAGGCTTTGA CTTCGGTGTT 
CGTCAGAATG GAGAGCGGGT TAACCACGTC AATCTTCCTC CCTGGGCACG CAACGATCCT 
CGGCTGTTCA TCCTTATTCA CCGGCAAGCA CTAGAGTCTG ACCATGTGTC CCAGAACATC 
TGTCACTGGA TCGACTTAGT GTTTGGCTAC AAGCAAAAGG GGAAGGCGTC TGTTCAAGCC 
ATCAATGTCT TCCACCCTGC TACATATTTT GGAATGGATG TCTCTGCAGT TGAAGATCCA 
GTGCAGAGAC GGGCTTTAGA AACCATGATA AAAACCTACG GGCAGACCCC ACGTCAGTTG 
TTCCACACAG CCCATGCCAG CCGACCTGGA GCCAAGCTTA ACATCGAAGG AGAGCTTCCA 
GCAGCTGTTG GCTTGTTAGT CCAGTTCGCT TTCAGAGAGA CCCGAGAACC AGTCAAGGAA 
GTCACTCATC CGAGCCCTTT GTCATGGATA AAAGGCTTGA AGTGGGGGGA GTACGTAGGT 
TCCCCCAGTG CTCCAGTACC TGTGGTCTGC TTCAGCCAGC CCCATGGAGA AAGATTTGGT 
TCCCTGCAGG CACTGCCCAC CAGAGCCATC TGTGGTTTAT CACGAAACTT CTGTCTTCTG 
ATGACCTACA ACAAGGAGCA AGGTGTGAGA AGCATGAACA ACACCAATAT TCAGTGGTCT 
GCTATCCTAA GCTGGGGATA TGCTGACAAC ATCTTACGGT TGAAAAGTAA GCAGAGTGAG 
CCACCAATCA ACTTCATTCA GAGTTCACAG CAGCACCAGG TAACCAGTTG TGCCTGGGTG 
CCTGACAGTT GTCAGCTCTT CACTGGGAGC AAGTGTGGTG TCATCACAGC CTATACCAAC 
AGGCTCACCA GCAGCACGCC CTCAGAAATT GAAATGGAGA GTCAGATGCA TCTCTATGGA 
CACACAGAGG AGATCACCGG CTTATGTGTC TGCAAGCCGT ACAGCGTGAT GATAAGCGTG 
AGCAGAGACG GGACCTGCAT AGTATGGGAC CTGAACAGGC TGTGCTATGT ACAAAGTTTG 
GCTGGACACA AAAGCCCTGT GACGGCTGTC TCTGCCAGTG AAACGTCAGG TGACATTGCT 
ACTGTGTGTG ACTCAGCTGG CGGGGGCAGT GACCTGAGAC TCTGGACCGT GAATGGGGAC 
CTCGTTGGAC ATGTCCACTG CAGAGAGATC ATTTGTTCTG TAGCTTTCTC CAACCAGCCT 
GAGGGAGTCT CCATCAACGT CATTGCTGGG GGATTAGAAA ATGGCATTGT AAGGCTATGG 
AGCACATGGG ACTTGAAGCC TGTGAGAGAG ATTACATTTC CCAAATCAAA TAAGCCCATC 
ATAAGCCTGA CATTCTCCTG TGATGGCCAC CATTTGTACA CTGCCAACAG TGAGGGGACA 
GTGATCGCAT GGTGCCGGAA GGACCAGCAG CGTGTGAAGC TGCCCATGTT CTACTCTTTC 
CTCAGCAGCT ACGCAGCTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lyst ( XM_006516561.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516624.1 Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X1, mRNA.

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CloneID	OMu48325
Clone ID Related Accession (Same CDS sequence)	XM_006516561.2 , XM_006516561.1
Accession Version	XM_006516561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysosomal-trafficking regulator isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGAAGGTGAT AGACCAGAAG TTACAGAATC CATCAATCCT 
GGTGACAGAC TCATAGAAGA CGGCTGTATT CATTTGATTT CACTGGGGTC CAAAGCATTG 
ATGATCCAAG TGTGGGCTGA TCCCCACAGT GGCACTTTTA TCTTTCGTGT GTGCATGGAC 
TCAAATGATG ACACGAAAGC TGTCTCACTA GCACAGGTGG AATCACAGGA GAATATTTTC 
TTTCCAAGCA AATGGCAACA CTTAGTACTT ACCTATATTC AGCATCCTCA AGGGAAAAAG 
AATGTCCATG GGGAAATCTC CATATGGGTC TCTGGGCAGA GGAAGACTGA TGTCATCTTG 
GATTTTGTGC TCCCAAGAAA AACAAGCTTA TCATCAGACA GCAATAAAAC ATTTTGCATG 
ATTGGTCATT GCTTAACATC CCAAGAAGAG TCTCTGCAAT TAGCTGGAAA ATGGGACCTG 
GGGAACTTGC TCCTCTTCAA TGGAGCTAAA ATTGGCTCAC AAGAGGCCTT TTTCCTGTAT 
GCTTGTGGAC CCAACTACAC ATCCATCATG CCGTGTAAAT ATGGACAGCC AGTCATTGAC 
TACTCCAAAT ACATTAATAA AGACATTTTG AGATGTGATG AAATCAGAGA CCTTTTTATG 
ACCAAGAAAG AAGTGGATGT TGGTCTCTTA ATTGAAAGTC TTTCAGTTGT TTATACAACT 
TGCTGTCCTG CTCAGTACAC CATCTATGAA CCAGTGATTC GACTCAAGGG CCAAGTGAAA 
ACTCAGCCCT CTCAAAGACC CTTCAGCTCA AAGGAAGCCC AGAGCATCTT GCTAGAACCT 
TCTCAACTCA AAGGCCTCCA ACCTACGGAA TGTAAAGCCA TCCAGGGCAT TCTGCATGAG 
ATTGGTGGGG CTGGCACATT TGTTTTTCTC TTTGCTAGGG TTGTTGAACT TAGTAGCTGT 
GAAGAAACTC AAGCATTAGC ACTGCGGGTT ATACTGTCTT TAATTAAGTA CAGCCAACAG 
AGAACACAGG AACTGGAAAA TTGTAATGGA CTCTCTATGA TTCACCAAGT GTTGGTCAAA 
CAGAAATGCA TTGTTGGCTT TCACATTTTG AAGACCCTTC TTGAAGGTTG CTGCGGTGAA 
GAAGTTATCC ACGTCAGTGA GCATGGAGAG TTCAAGCTGG ATGTTGAGTC TCATGCTATA 
ATCCAAGATG TTAAGCTGCT GCAGGAACTG TTACTTGACT GGAAGATATG GAATAAGGCA 
GAGCAAGGTG TGTGGGAGAC TCTGCTAGCA GCTTTGGAAG TCCTCATCCG GGTAGAGCAC 
CACCAGCAGC AGTTTAATAT TAAGCAGTTG CTGAACGCCC ACGTGGTTCA CCACTTCCTA 
CTGACCTGTC AGGTTTTACA GGAACACAGA GAGGGGCAGC TTACATCTAT GCCCCGAGAA 
GTTTGTAGAT CATTTGTGAA AATCATTGCA GAAGTCCTTG GTTCTCCTCC AGACTTGGAA 
TTATTGACAG TTATTTTCAA TTTCCTGTTA GCTGTACACC CTCCTACTAA TACTTATGTT 
TGTCACAATC CCACAAACTT CTACTTCTCT TTGCACATAG ATGGCAAGAT CTTTCAGGAG 
AAAGTGCAGT CACTCGCGTA CCTGAGGCAT TCTAGCAGCG GAGGGCAAGC CTTTCCCAGC 
CCTGGATTCC TGGTAATAAG CCCATCTGCC TTTACTGCAG CTCCTCCTGA AGGAACCAGT 
TCTTCCAATA TTGTTCCACA GCGGATGGCT GCTCAGATGG TTCGATCTAG AAGTCTACCA 
GCATTTCCTA CTTATTTACC ACTAATACGA GCACAAAAAC TGGCTGCAAG TTTGGGTTTT 
AGTGTTGACA AGTTACAAAA TATTGCAGAT GCCAACCCAG AGAAACAGAA TCTTTTAGGA 
AGACCCTACG CACTGAAAAC AAGCAAAGAG GAAGCATTCA TCAGCAGCTG TGAGTCTGCA 
AAGACTGTTT GTGAAATGGA GGCTCTTCTT GGAGCCCACG CCTCTGCCAA TGGGGTTTCC 
AGAGGATCAC CGAGGTTCCC CAGGGCCAGA GTAGATCACA AAGATGTGGG AACAGAGCCC 
AGATCAGATG ATGACAGTCC TGGGGATGAG TCTTACCCAC GTCGGCCTGA CAACCTCAAG 
GGACTGGCCT CATTCCAGCG AAGCCAAAGC ACTGTCGCAA GCCTTGGGCT GGCGTTTCCC 
TCTCAGAATG GATCTGCAGT TGCTAGCAGG TGGCCAAGTC TTGTTGATAG GAATGCTGAT 
GACTGGGAGA ACTTTACCTT TTCTCCTGCT TATGAGGCAA GCTACAACCG AGCCACAAGC 
ACCCACAGTG TCATTGAAGA CTGTCTGATA CCTATCTGCT GTGGATTATA TGAACTCTTA 
AGTGGGGTTC TTCTTGTCCT GCCTGATGCT ATGCTTGAAG ATGTGATGGA CAGGATTATT 
CAAGCAGATA TTCTTCTAGT CCTTGTTAAC CACCCATCAC CTGCTATCCA GCAAGGAGTA 
ATTAAACTGT TACATGCATA CATTAATAGA GCATCAAAGG AGCAAAAGGA CAAGTTTCTG 
AAGAACCGTG GCTTTTCCTT ATTAGCCAAC CAGTTGTATC TTCATAGGGG AACTCAGGAG 
TTGTTGGAGT GCTTTGTTGA AATGTTCTTT GGTCGACCGA TTGGCCTGGA TGAAGAATTT 
GATCTGGAGG AAGTGAAGCA CATGGAACTG TTCCAGAAGT GGTCTGTCAT TCCCGTTCTC 
GGACTAATAG AGACCTCTCT CTATGACAAT GTCCTCTTGC ACAATGCTCT TTTACTTCTT 
CTGCAAGTTT TAAACTCTTG TTCCAAGGTA GCAGACATGC TACTGGACAA TGGTCTACTC 
TATGTATTAT GTAATACAGT AGCAGCCCTG AATGGATTAG AAAAGAACAT TCCTGTGAAC 
GAATACAAAT TGCTCGCATG TGATATACAG CAGCTTTTCA TAGCAGTTAC AATTCATGCT 
TGCAGTTCCT CAGGCACACA GTATTTTAGA GTGATTGAAG ACCTTATTGT ACTTCTTGGA 
TATCTTCATA ATAGCAAAAA CAAGAGGACA CAAAATATGG CTTTGGCCCT GCAGCTTAGA 
GTTCTCCAGG CTGCTTTGGA ATTTATAAGG AGCACAGCCA ATCATGACTC TGAAAGTCCA 
GTGCACTCGC CTTCTGCCCA CCGCCATTCA GTGCCTCCGA AGCGGAGAAG CATTGCTGGT 
TCAATTCCAG TGAAGGGTTC TGTTACTCCT TATCCACCAA GACGTCACTT TGGTTCCTAT 
GGTCAACTTC CGATGCCCTT CTTCAGTCCA TTAGATCAAG ACTCCCCTCA GCTCTCTCCT 
GGATGCACCA CATGTGCCGC ATCCCCCAGA GCAGGTTCTC GCAAATTCCC TCTGGCTCAG 
ACAGAGTCTC TGCTGATGAA GATGCGCTCA GTGGCCAGCG ATGAGCTACA CTCTATGATG 
CAGAGGAGGA TGAGCCAAGA GCACCCCAGC CAGGCCTCGG AGGCAGAGCT CGCTCAGAGG 
CTGCAGAGGC TCACCATCTT AGCTGTGAAC AGGATTATTT ACCAAGAGTT GAATTCAGAT 
ATTATTGACA TTTTGAGAAC TCCAGAAAAT ACATCCCAAA GCAAGACCTC AGTTTCTCAG 
ACTGAAATTT CTGAAGAAGA CATGCATCAT GAGCAACCTT CTGTATATAA TCCATTTCAA 
AAAGAAATGT TAACCTATCT GTTGGATGGC TTCAAAGTGT GTATTGGTTC AAGTAAAACT 
AGCGTTTCTA AGCAGCAGTG GACTAAAATC CTGGGGTCTT GTAAAGAAAC CCTCCGAGAC 
CAGCTTGGAA GATTGCTAGC GCATATTTTG TCTCCAACCC ACACTGTACA AGAACGGAAG 
CAGATACTTG AGATAGTTCA TGAACCAGCT CACCAGGATA TACTTCGTGA CTGTCTTAGC 
CCCTCCCCAC AACATGGAGC CAAGTTGGTT TTGTATTTGT CAGAGTTGAT ACATAATCAT 
CAGGATGAGT TAAGTGAAGA AGAAATGGAC ACAGCAGAAC TGCTTATGAA TGCTCTAAAG 
TTATGTGGCC ACAAGTGCAT CCCGCCCAGT GCCCCTTCCA AACCAGAGCT CATTAAGATC 
ATCAGAGAGG AGCAAAAGAA GTATGAAAGT GAAGAGAGTG TGAGCAAAGG CTCATGGCAG 
AAAACGGTGA ACAACAACCA GCAAAGTCTC TTCCAGAGGC TCGATTTCAA ATCCAAGGAT 
ATATCTAAAA TCGCTGCAGA CATCACCCAG GCTGTATCAC TCTCCCAAGG CATTGAAAGG 
AAGAAGGTGA TCCAGCACAT CAGAGGGATG TACAAAGTTG ACCTGAGTGC CAGCAGGCAC 
TGGCAGGAAT GCATCCAGCA GCTGACACAT GACAGAGCAG TCTGGTATGA CCCAATCTAC 
TATCCAACTT CATGGCAGTT GGATCCAACA GAAGGGCCAA ACCGAGAGAG GAGACGTTTG 
CAGAGATGCT ATCTAACTAT TCCCAATAAG TACCTCCTGA GGGACAGACA GAAGTCAGAA 
GGTGTGCTCA GGCCCCCACT CTCTTACCTT TTTGAAGATA AAACTCATTC TTCCTTCTCC 
TCTACTGTCA AAGACAAAGC TGCAAGTGAA TCCATCAGAG TGAATCGAAG ATGTATCAGT 
GTTGCACCAT CTAGAGAGAC AGCTGGGGAA TTGTTGTTAG GTAAATGTGG GATGTACTTT 
GTGGAAGACA ATGCCTCTGA CGCAGTTGAA AGCTCGAGCC TCCAAGGGGA GTTAGAGCCG 
GCATCATTTT CTTGGACATA TGAGGAAATT AAAGAAGTTC ACAGGCGCTG GTGGCAACTA 
AGAGATAATG CTGTAGAAAT CTTTTTAACA AATGGCAGAA CACTCCTATT AGCATTTGAC 
AATAACAAGG TTCGTGATGA CGTGTACCAG AGCATCCTCA CAAATAACCT CCCAAATCTT 
CTGGAGTACG GCAACATCAC CGCTCTGACA AACCTGTGGT ATTCTGGACA AATTACCAAT 
TTTGAATATT TGACTCATTT AAACAAGCAT GCGGGCCGGT CCTTCAATGA TCTCATGCAG 
TACCCGGTGT TCCCCTTCAT CCTTTCTGAC TATGTTAGTG AGACTCTTGA CCTCAATGAT 
CCATCTATCT ACAGAAACCT ATCTAAGCCT ATAGCTGTGC AGTATAAAGA AAAAGAAGAC 
CGTTACGTTG ACACATACAA GTACTTGGAG GAGGAGTATC GCAAGGGAGC TCGAGAGGAT 
GACCCCATGC CTCCTGTGCA ACCCTACCAC TATGGCTCCC ACTACTCCAA CAGCGGCACC 
GTGCTCCACT TCCTGGTCAG GATGCCGCCT TTCACTAAAA TGTTTCTAGC CTATCAAGAT 
CAGAGTTTCG ACATTCCAGA CCGAACATTT CATTCTACAA ACACAACTTG GCGCCTCTCC 
TCCTTTGAGT CCATGACTGA TGTGAAGGAG CTGATTCCAG AGTTTTTCTA TCTTCCTGAG 
TTCTTAGTGA ACCGTGAAGG CTTTGACTTC GGTGTTCGTC AGAATGGAGA GCGGGTTAAC 
CACGTCAATC TTCCTCCCTG GGCACGCAAC GATCCTCGGC TGTTCATCCT TATTCACCGG 
CAAGCACTAG AGTCTGACCA TGTGTCCCAG AACATCTGTC ACTGGATCGA CTTAGTGTTT 
GGCTACAAGC AAAAGGGGAA GGCGTCTGTT CAAGCCATCA ATGTCTTCCA CCCTGCTACA 
TATTTTGGAA TGGATGTCTC TGCAGTTGAA GATCCAGTGC AGAGACGGGC TTTAGAAACC 
ATGATAAAAA CCTACGGGCA GACCCCACGT CAGTTGTTCC ACACAGCCCA TGCCAGCCGA 
CCTGGAGCCA AGCTTAACAT CGAAGGAGAG CTTCCAGCAG CTGTTGGCTT GTTAGTCCAG 
TTCGCTTTCA GAGAGACCCG AGAACCAGTC AAGGAAGTCA CTCATCCGAG CCCTTTGTCA 
TGGATAAAAG GCTTGAAGTG GGGGGAGTAC GTAGGTTCCC CCAGTGCTCC AGTACCTGTG 
GTCTGCTTCA GCCAGCCCCA TGGAGAAAGA TTTGGTTCCC TGCAGGCACT GCCCACCAGA 
GCCATCTGTG GTTTATCACG AAACTTCTGT CTTCTGATGA CCTACAACAA GGAGCAAGGT 
GTGAGAAGCA TGAACAACAC CAATATTCAG TGGTCTGCTA TCCTAAGCTG GGGATATGCT 
GACAACATCT TACGGTTGAA AAGTAAGCAG AGTGAGCCAC CAATCAACTT CATTCAGAGT 
TCACAGCAGC ACCAGGTAAC CAGTTGTGCC TGGGTGCCTG ACAGTTGTCA GCTCTTCACT 
GGGAGCAAGT GTGGTGTCAT CACAGCCTAT ACCAACAGGC TCACCAGCAG CACGCCCTCA 
GAAATTGAAA TGGAGAGTCA GATGCATCTC TATGGACACA CAGAGGAGAT CACCGGCTTA 
TGTGTCTGCA AGCCGTACAG CGTGATGATA AGCGTGAGCA GAGACGGGAC CTGCATAGTA 
TGGGACCTGA ACAGGCTGTG CTATGTACAA AGTTTGGCTG GACACAAAAG CCCTGTGACG 
GCTGTCTCTG CCAGTGAAAC GTCAGGTGAC ATTGCTACTG TGTGTGACTC AGCTGGCGGG 
GGCAGTGACC TGAGACTCTG GACCGTGAAT GGGGACCTCG TTGGACATGT CCACTGCAGA 
GAGATCATTT GTTCTGTAGC TTTCTCCAAC CAGCCTGAGG GAGTCTCCAT CAACGTCATT 
GCTGGGGGAT TAGAAAATGG CATTGTAAGG CTATGGAGCA CATGGGACTT GAAGCCTGTG 
AGAGAGATTA CATTTCCCAA ATCAAATAAG CCCATCATAA GCCTGACATT CTCCTGTGAT 
GGCCACCATT TGTACACTGC CAACAGTGAG GGGACAGTGA TCGCATGGTG CCGGAAGGAC 
CAGCAGCGTG TGAAGCTGCC CATGTTCTAC TCTTTCCTCA GCAGCTACGC AGCTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516624.1
CDS	280..11799
Translation	MSTDSNSLAREFLIDVNQLCNAVVQRAEAREEEEEETHMATLGQYLVHGRGFLLLTKLNSIIDQALTCREELLTLLLSLLPLVWKIPVQEQQATDFNLPLSSDIILTKEKNSSLQKSTQGKLYLEGSAPSGQVSAKVNLFRKIRRQRKSTHRYSVRDARKTQLSTSDSEGNSDEKSTVVSKHRRLHALPRFLTQSPKEGHLVAKPDPSATKEQVLSDTMSVENSREVILRQDSNGDILSEPAALSILSNMNNSPFDLCHVLLSLLEKVCKFDIALNHNSSLALSVVPTLTEFLAGFGDCCNQSDTLEGQLVSAGWTEEPVALVQRMLFRTVLHLMSVDVSTAEAMPESLRKNLTELLRAALKIRACLEKQPEPFSPRQKKTLQEVQEGFVFSKYRHRALLLPELLEGVLQLLISCLQSAASNPFYFSQAMDLVQEFIQHQGFNLFETAVLQMEWLLSRDGVPSEAAEHLKALINSVIKIMSTVKKVKSEQLHHSMCTRKRHRRCEYSHFMQHHRDLSGLLVSAFKNQLSKSPFEETAEGDVQYPERCCCIAVCAHQCLRLLQQVSLSTTCVQILSGVHSVGICCCMDPKSVIVPLLHAFKLPALKAFQQHILNVLSKLLLDQLGGAELSPRIKKAACNICTVDSDQLAKLGETLQGTLCGAGPTSGLPSPSYRFQGILPSSGSEDLLWKWDALEAYQSFVFQEDRLHNIQIANHICNLIQKGNVVVQWKLYNYIFNPVLQRGVELVHHCQQLSIPSAQTHMCSQLKQYLPQEVLQIYLKTLPVLLKSRVIRDLFLSCNGVNQIIELNYLDGIRSHSLKAFETLIVSLGEQQKDAAVLDVDGLDIQQELPSLSVGPSLHKQQASSDSPCSLRKFYASLREPDPKKRKTIHQDVHINTINLFLCVAFLCVSKEADSDRESANESEDTSGYDSTASEPLSHMLPCLSLENVVLPSPECLHHAADIWSMCRWIYMLNSVFQKQFHRLGGFQVCHELIFMIIQKLFRSHTEDQGRRQGEMSRNENQELIRISYPELTLKGDVSSATAPDLGFLRKSADSVRGFQSQPVLPTSAEQIVATESVPGERKAFMSQQSETSLQSIRLLESLLAICLHSARACQQKMELELPSQGLSVENILCELREHLSQSKVAETELAKPLFDALLRVALGNHSADLGPGDAVTEKSHPSEEELLSQPGDFSEEAEDSQCCSLKLLGEEEGYEADSESNPEDVDTQDDGVELNPEAEGFSGSIVSNNLLENLTHGEIIYPEICMLGLNLLSASKAKLDVLAHVFESFLKIVRQKEKNISLLIQQGTVKILLGGFLNILTQTNSDFQACQRVLVDLLVSLMSSRTCSEDLTLLLRIFLEKSPCTEILLLGIHKIVESDFTMSPSQCLTFPFLHTPSLSNGVLSQKPPGILNSKALGLLRRARISRGKKEADRESFPYRLLSSWHIAPIHLPLLGQNCWPHLSEGFSVSLWFNVEYIHESESAAERGKRVKKRNKPSVLEDSSFEGAEGDRPEVTESINPGDRLIEDGCIHLISLGSKALMIQVWADPHSGTFIFRVCMDSNDDTKAVSLAQVESQENIFFPSKWQHLVLTYIQHPQGKKNVHGEISIWVSGQRKTDVILDFVLPRKTSLSSDSNKTFCMIGHCLTSQEESLQLAGKWDLGNLLLFNGAKIGSQEAFFLYACGPNYTSIMPCKYGQPVIDYSKYINKDILRCDEIRDLFMTKKEVDVGLLIESLSVVYTTCCPAQYTIYEPVIRLKGQVKTQPSQRPFSSKEAQSILLEPSQLKGLQPTECKAIQGILHEIGGAGTFVFLFARVVELSSCEETQALALRVILSLIKYSQQRTQELENCNGLSMIHQVLVKQKCIVGFHILKTLLEGCCGEEVIHVSEHGEFKLDVESHAIIQDVKLLQELLLDWKIWNKAEQGVWETLLAALEVLIRVEHHQQQFNIKQLLNAHVVHHFLLTCQVLQEHREGQLTSMPREVCRSFVKIIAEVLGSPPDLELLTVIFNFLLAVHPPTNTYVCHNPTNFYFSLHIDGKIFQEKVQSLAYLRHSSSGGQAFPSPGFLVISPSAFTAAPPEGTSSSNIVPQRMAAQMVRSRSLPAFPTYLPLIRAQKLAASLGFSVDKLQNIADANPEKQNLLGRPYALKTSKEEAFISSCESAKTVCEMEALLGAHASANGVSRGSPRFPRARVDHKDVGTEPRSDDDSPGDESYPRRPDNLKGLASFQRSQSTVASLGLAFPSQNGSAVASRWPSLVDRNADDWENFTFSPAYEASYNRATSTHSVIEDCLIPICCGLYELLSGVLLVLPDAMLEDVMDRIIQADILLVLVNHPSPAIQQGVIKLLHAYINRASKEQKDKFLKNRGFSLLANQLYLHRGTQELLECFVEMFFGRPIGLDEEFDLEEVKHMELFQKWSVIPVLGLIETSLYDNVLLHNALLLLLQVLNSCSKVADMLLDNGLLYVLCNTVAALNGLEKNIPVNEYKLLACDIQQLFIAVTIHACSSSGTQYFRVIEDLIVLLGYLHNSKNKRTQNMALALQLRVLQAALEFIRSTANHDSESPVHSPSAHRHSVPPKRRSIAGSIPVKGSVTPYPPRRHFGSYGQLPMPFFSPLDQDSPQLSPGCTTCAASPRAGSRKFPLAQTESLLMKMRSVASDELHSMMQRRMSQEHPSQASEAELAQRLQRLTILAVNRIIYQELNSDIIDILRTPENTSQSKTSVSQTEISEEDMHHEQPSVYNPFQKEMLTYLLDGFKVCIGSSKTSVSKQQWTKILGSCKETLRDQLGRLLAHILSPTHTVQERKQILEIVHEPAHQDILRDCLSPSPQHGAKLVLYLSELIHNHQDELSEEEMDTAELLMNALKLCGHKCIPPSAPSKPELIKIIREEQKKYESEESVSKGSWQKTVNNNQQSLFQRLDFKSKDISKIAADITQAVSLSQGIERKKVIQHIRGMYKVDLSASRHWQECIQQLTHDRAVWYDPIYYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLRDRQKSEGVLRPPLSYLFEDKTHSSFSSTVKDKAASESIRVNRRCISVAPSRETAGELLLGKCGMYFVEDNASDAVESSSLQGELEPASFSWTYEEIKEVHRRWWQLRDNAVEIFLTNGRTLLLAFDNNKVRDDVYQSILTNNLPNLLEYGNITALTNLWYSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILSDYVSETLDLNDPSIYRNLSKPIAVQYKEKEDRYVDTYKYLEEEYRKGAREDDPMPPVQPYHYGSHYSNSGTVLHFLVRMPPFTKMFLAYQDQSFDIPDRTFHSTNTTWRLSSFESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNGERVNHVNLPPWARNDPRLFILIHRQALESDHVSQNICHWIDLVFGYKQKGKASVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFHTAHASRPGAKLNIEGELPAAVGLLVQFAFRETREPVKEVTHPSPLSWIKGLKWGEYVGSPSAPVPVVCFSQPHGERFGSLQALPTRAICGLSRNFCLLMTYNKEQGVRSMNNTNIQWSAILSWGYADNILRLKSKQSEPPINFIQSSQQHQVTSCAWVPDSCQLFTGSKCGVITAYTNRLTSSTPSEIEMESQMHLYGHTEEITGLCVCKPYSVMISVSRDGTCIVWDLNRLCYVQSLAGHKSPVTAVSASETSGDIATVCDSAGGGSDLRLWTVNGDLVGHVHCREIICSVAFSNQPEGVSINVIAGGLENGIVRLWSTWDLKPVREITFPKSNKPIISLTFSCDGHHLYTANSEGTVIAWCRKDQQRVKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_006516561.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516561.2

ORF Insert Sequence:

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGAAGGTGAT AGACCAGAAG TTACAGAATC CATCAATCCT 
GGTGACAGAC TCATAGAAGA CGGCTGTATT CATTTGATTT CACTGGGGTC CAAAGCATTG 
ATGATCCAAG TGTGGGCTGA TCCCCACAGT GGCACTTTTA TCTTTCGTGT GTGCATGGAC 
TCAAATGATG ACACGAAAGC TGTCTCACTA GCACAGGTGG AATCACAGGA GAATATTTTC 
TTTCCAAGCA AATGGCAACA CTTAGTACTT ACCTATATTC AGCATCCTCA AGGGAAAAAG 
AATGTCCATG GGGAAATCTC CATATGGGTC TCTGGGCAGA GGAAGACTGA TGTCATCTTG 
GATTTTGTGC TCCCAAGAAA AACAAGCTTA TCATCAGACA GCAATAAAAC ATTTTGCATG 
ATTGGTCATT GCTTAACATC CCAAGAAGAG TCTCTGCAAT TAGCTGGAAA ATGGGACCTG 
GGGAACTTGC TCCTCTTCAA TGGAGCTAAA ATTGGCTCAC AAGAGGCCTT TTTCCTGTAT 
GCTTGTGGAC CCAACTACAC ATCCATCATG CCGTGTAAAT ATGGACAGCC AGTCATTGAC 
TACTCCAAAT ACATTAATAA AGACATTTTG AGATGTGATG AAATCAGAGA CCTTTTTATG 
ACCAAGAAAG AAGTGGATGT TGGTCTCTTA ATTGAAAGTC TTTCAGTTGT TTATACAACT 
TGCTGTCCTG CTCAGTACAC CATCTATGAA CCAGTGATTC GACTCAAGGG CCAAGTGAAA 
ACTCAGCCCT CTCAAAGACC CTTCAGCTCA AAGGAAGCCC AGAGCATCTT GCTAGAACCT 
TCTCAACTCA AAGGCCTCCA ACCTACGGAA TGTAAAGCCA TCCAGGGCAT TCTGCATGAG 
ATTGGTGGGG CTGGCACATT TGTTTTTCTC TTTGCTAGGG TTGTTGAACT TAGTAGCTGT 
GAAGAAACTC AAGCATTAGC ACTGCGGGTT ATACTGTCTT TAATTAAGTA CAGCCAACAG 
AGAACACAGG AACTGGAAAA TTGTAATGGA CTCTCTATGA TTCACCAAGT GTTGGTCAAA 
CAGAAATGCA TTGTTGGCTT TCACATTTTG AAGACCCTTC TTGAAGGTTG CTGCGGTGAA 
GAAGTTATCC ACGTCAGTGA GCATGGAGAG TTCAAGCTGG ATGTTGAGTC TCATGCTATA 
ATCCAAGATG TTAAGCTGCT GCAGGAACTG TTACTTGACT GGAAGATATG GAATAAGGCA 
GAGCAAGGTG TGTGGGAGAC TCTGCTAGCA GCTTTGGAAG TCCTCATCCG GGTAGAGCAC 
CACCAGCAGC AGTTTAATAT TAAGCAGTTG CTGAACGCCC ACGTGGTTCA CCACTTCCTA 
CTGACCTGTC AGGTTTTACA GGAACACAGA GAGGGGCAGC TTACATCTAT GCCCCGAGAA 
GTTTGTAGAT CATTTGTGAA AATCATTGCA GAAGTCCTTG GTTCTCCTCC AGACTTGGAA 
TTATTGACAG TTATTTTCAA TTTCCTGTTA GCTGTACACC CTCCTACTAA TACTTATGTT 
TGTCACAATC CCACAAACTT CTACTTCTCT TTGCACATAG ATGGCAAGAT CTTTCAGGAG 
AAAGTGCAGT CACTCGCGTA CCTGAGGCAT TCTAGCAGCG GAGGGCAAGC CTTTCCCAGC 
CCTGGATTCC TGGTAATAAG CCCATCTGCC TTTACTGCAG CTCCTCCTGA AGGAACCAGT 
TCTTCCAATA TTGTTCCACA GCGGATGGCT GCTCAGATGG TTCGATCTAG AAGTCTACCA 
GCATTTCCTA CTTATTTACC ACTAATACGA GCACAAAAAC TGGCTGCAAG TTTGGGTTTT 
AGTGTTGACA AGTTACAAAA TATTGCAGAT GCCAACCCAG AGAAACAGAA TCTTTTAGGA 
AGACCCTACG CACTGAAAAC AAGCAAAGAG GAAGCATTCA TCAGCAGCTG TGAGTCTGCA 
AAGACTGTTT GTGAAATGGA GGCTCTTCTT GGAGCCCACG CCTCTGCCAA TGGGGTTTCC 
AGAGGATCAC CGAGGTTCCC CAGGGCCAGA GTAGATCACA AAGATGTGGG AACAGAGCCC 
AGATCAGATG ATGACAGTCC TGGGGATGAG TCTTACCCAC GTCGGCCTGA CAACCTCAAG 
GGACTGGCCT CATTCCAGCG AAGCCAAAGC ACTGTCGCAA GCCTTGGGCT GGCGTTTCCC 
TCTCAGAATG GATCTGCAGT TGCTAGCAGG TGGCCAAGTC TTGTTGATAG GAATGCTGAT 
GACTGGGAGA ACTTTACCTT TTCTCCTGCT TATGAGGCAA GCTACAACCG AGCCACAAGC 
ACCCACAGTG TCATTGAAGA CTGTCTGATA CCTATCTGCT GTGGATTATA TGAACTCTTA 
AGTGGGGTTC TTCTTGTCCT GCCTGATGCT ATGCTTGAAG ATGTGATGGA CAGGATTATT 
CAAGCAGATA TTCTTCTAGT CCTTGTTAAC CACCCATCAC CTGCTATCCA GCAAGGAGTA 
ATTAAACTGT TACATGCATA CATTAATAGA GCATCAAAGG AGCAAAAGGA CAAGTTTCTG 
AAGAACCGTG GCTTTTCCTT ATTAGCCAAC CAGTTGTATC TTCATAGGGG AACTCAGGAG 
TTGTTGGAGT GCTTTGTTGA AATGTTCTTT GGTCGACCGA TTGGCCTGGA TGAAGAATTT 
GATCTGGAGG AAGTGAAGCA CATGGAACTG TTCCAGAAGT GGTCTGTCAT TCCCGTTCTC 
GGACTAATAG AGACCTCTCT CTATGACAAT GTCCTCTTGC ACAATGCTCT TTTACTTCTT 
CTGCAAGTTT TAAACTCTTG TTCCAAGGTA GCAGACATGC TACTGGACAA TGGTCTACTC 
TATGTATTAT GTAATACAGT AGCAGCCCTG AATGGATTAG AAAAGAACAT TCCTGTGAAC 
GAATACAAAT TGCTCGCATG TGATATACAG CAGCTTTTCA TAGCAGTTAC AATTCATGCT 
TGCAGTTCCT CAGGCACACA GTATTTTAGA GTGATTGAAG ACCTTATTGT ACTTCTTGGA 
TATCTTCATA ATAGCAAAAA CAAGAGGACA CAAAATATGG CTTTGGCCCT GCAGCTTAGA 
GTTCTCCAGG CTGCTTTGGA ATTTATAAGG AGCACAGCCA ATCATGACTC TGAAAGTCCA 
GTGCACTCGC CTTCTGCCCA CCGCCATTCA GTGCCTCCGA AGCGGAGAAG CATTGCTGGT 
TCAATTCCAG TGAAGGGTTC TGTTACTCCT TATCCACCAA GACGTCACTT TGGTTCCTAT 
GGTCAACTTC CGATGCCCTT CTTCAGTCCA TTAGATCAAG ACTCCCCTCA GCTCTCTCCT 
GGATGCACCA CATGTGCCGC ATCCCCCAGA GCAGGTTCTC GCAAATTCCC TCTGGCTCAG 
ACAGAGTCTC TGCTGATGAA GATGCGCTCA GTGGCCAGCG ATGAGCTACA CTCTATGATG 
CAGAGGAGGA TGAGCCAAGA GCACCCCAGC CAGGCCTCGG AGGCAGAGCT CGCTCAGAGG 
CTGCAGAGGC TCACCATCTT AGCTGTGAAC AGGATTATTT ACCAAGAGTT GAATTCAGAT 
ATTATTGACA TTTTGAGAAC TCCAGAAAAT ACATCCCAAA GCAAGACCTC AGTTTCTCAG 
ACTGAAATTT CTGAAGAAGA CATGCATCAT GAGCAACCTT CTGTATATAA TCCATTTCAA 
AAAGAAATGT TAACCTATCT GTTGGATGGC TTCAAAGTGT GTATTGGTTC AAGTAAAACT 
AGCGTTTCTA AGCAGCAGTG GACTAAAATC CTGGGGTCTT GTAAAGAAAC CCTCCGAGAC 
CAGCTTGGAA GATTGCTAGC GCATATTTTG TCTCCAACCC ACACTGTACA AGAACGGAAG 
CAGATACTTG AGATAGTTCA TGAACCAGCT CACCAGGATA TACTTCGTGA CTGTCTTAGC 
CCCTCCCCAC AACATGGAGC CAAGTTGGTT TTGTATTTGT CAGAGTTGAT ACATAATCAT 
CAGGATGAGT TAAGTGAAGA AGAAATGGAC ACAGCAGAAC TGCTTATGAA TGCTCTAAAG 
TTATGTGGCC ACAAGTGCAT CCCGCCCAGT GCCCCTTCCA AACCAGAGCT CATTAAGATC 
ATCAGAGAGG AGCAAAAGAA GTATGAAAGT GAAGAGAGTG TGAGCAAAGG CTCATGGCAG 
AAAACGGTGA ACAACAACCA GCAAAGTCTC TTCCAGAGGC TCGATTTCAA ATCCAAGGAT 
ATATCTAAAA TCGCTGCAGA CATCACCCAG GCTGTATCAC TCTCCCAAGG CATTGAAAGG 
AAGAAGGTGA TCCAGCACAT CAGAGGGATG TACAAAGTTG ACCTGAGTGC CAGCAGGCAC 
TGGCAGGAAT GCATCCAGCA GCTGACACAT GACAGAGCAG TCTGGTATGA CCCAATCTAC 
TATCCAACTT CATGGCAGTT GGATCCAACA GAAGGGCCAA ACCGAGAGAG GAGACGTTTG 
CAGAGATGCT ATCTAACTAT TCCCAATAAG TACCTCCTGA GGGACAGACA GAAGTCAGAA 
GGTGTGCTCA GGCCCCCACT CTCTTACCTT TTTGAAGATA AAACTCATTC TTCCTTCTCC 
TCTACTGTCA AAGACAAAGC TGCAAGTGAA TCCATCAGAG TGAATCGAAG ATGTATCAGT 
GTTGCACCAT CTAGAGAGAC AGCTGGGGAA TTGTTGTTAG GTAAATGTGG GATGTACTTT 
GTGGAAGACA ATGCCTCTGA CGCAGTTGAA AGCTCGAGCC TCCAAGGGGA GTTAGAGCCG 
GCATCATTTT CTTGGACATA TGAGGAAATT AAAGAAGTTC ACAGGCGCTG GTGGCAACTA 
AGAGATAATG CTGTAGAAAT CTTTTTAACA AATGGCAGAA CACTCCTATT AGCATTTGAC 
AATAACAAGG TTCGTGATGA CGTGTACCAG AGCATCCTCA CAAATAACCT CCCAAATCTT 
CTGGAGTACG GCAACATCAC CGCTCTGACA AACCTGTGGT ATTCTGGACA AATTACCAAT 
TTTGAATATT TGACTCATTT AAACAAGCAT GCGGGCCGGT CCTTCAATGA TCTCATGCAG 
TACCCGGTGT TCCCCTTCAT CCTTTCTGAC TATGTTAGTG AGACTCTTGA CCTCAATGAT 
CCATCTATCT ACAGAAACCT ATCTAAGCCT ATAGCTGTGC AGTATAAAGA AAAAGAAGAC 
CGTTACGTTG ACACATACAA GTACTTGGAG GAGGAGTATC GCAAGGGAGC TCGAGAGGAT 
GACCCCATGC CTCCTGTGCA ACCCTACCAC TATGGCTCCC ACTACTCCAA CAGCGGCACC 
GTGCTCCACT TCCTGGTCAG GATGCCGCCT TTCACTAAAA TGTTTCTAGC CTATCAAGAT 
CAGAGTTTCG ACATTCCAGA CCGAACATTT CATTCTACAA ACACAACTTG GCGCCTCTCC 
TCCTTTGAGT CCATGACTGA TGTGAAGGAG CTGATTCCAG AGTTTTTCTA TCTTCCTGAG 
TTCTTAGTGA ACCGTGAAGG CTTTGACTTC GGTGTTCGTC AGAATGGAGA GCGGGTTAAC 
CACGTCAATC TTCCTCCCTG GGCACGCAAC GATCCTCGGC TGTTCATCCT TATTCACCGG 
CAAGCACTAG AGTCTGACCA TGTGTCCCAG AACATCTGTC ACTGGATCGA CTTAGTGTTT 
GGCTACAAGC AAAAGGGGAA GGCGTCTGTT CAAGCCATCA ATGTCTTCCA CCCTGCTACA 
TATTTTGGAA TGGATGTCTC TGCAGTTGAA GATCCAGTGC AGAGACGGGC TTTAGAAACC 
ATGATAAAAA CCTACGGGCA GACCCCACGT CAGTTGTTCC ACACAGCCCA TGCCAGCCGA 
CCTGGAGCCA AGCTTAACAT CGAAGGAGAG CTTCCAGCAG CTGTTGGCTT GTTAGTCCAG 
TTCGCTTTCA GAGAGACCCG AGAACCAGTC AAGGAAGTCA CTCATCCGAG CCCTTTGTCA 
TGGATAAAAG GCTTGAAGTG GGGGGAGTAC GTAGGTTCCC CCAGTGCTCC AGTACCTGTG 
GTCTGCTTCA GCCAGCCCCA TGGAGAAAGA TTTGGTTCCC TGCAGGCACT GCCCACCAGA 
GCCATCTGTG GTTTATCACG AAACTTCTGT CTTCTGATGA CCTACAACAA GGAGCAAGGT 
GTGAGAAGCA TGAACAACAC CAATATTCAG TGGTCTGCTA TCCTAAGCTG GGGATATGCT 
GACAACATCT TACGGTTGAA AAGTAAGCAG AGTGAGCCAC CAATCAACTT CATTCAGAGT 
TCACAGCAGC ACCAGGTAAC CAGTTGTGCC TGGGTGCCTG ACAGTTGTCA GCTCTTCACT 
GGGAGCAAGT GTGGTGTCAT CACAGCCTAT ACCAACAGGC TCACCAGCAG CACGCCCTCA 
GAAATTGAAA TGGAGAGTCA GATGCATCTC TATGGACACA CAGAGGAGAT CACCGGCTTA 
TGTGTCTGCA AGCCGTACAG CGTGATGATA AGCGTGAGCA GAGACGGGAC CTGCATAGTA 
TGGGACCTGA ACAGGCTGTG CTATGTACAA AGTTTGGCTG GACACAAAAG CCCTGTGACG 
GCTGTCTCTG CCAGTGAAAC GTCAGGTGAC ATTGCTACTG TGTGTGACTC AGCTGGCGGG 
GGCAGTGACC TGAGACTCTG GACCGTGAAT GGGGACCTCG TTGGACATGT CCACTGCAGA 
GAGATCATTT GTTCTGTAGC TTTCTCCAAC CAGCCTGAGG GAGTCTCCAT CAACGTCATT 
GCTGGGGGAT TAGAAAATGG CATTGTAAGG CTATGGAGCA CATGGGACTT GAAGCCTGTG 
AGAGAGATTA CATTTCCCAA ATCAAATAAG CCCATCATAA GCCTGACATT CTCCTGTGAT 
GGCCACCATT TGTACACTGC CAACAGTGAG GGGACAGTGA TCGCATGGTG CCGGAAGGAC 
CAGCAGCGTG TGAAGCTGCC CATGTTCTAC TCTTTCCTCA GCAGCTACGC AGCTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lyst ( XM_006516561.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516624.1 Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X1, mRNA.

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CloneID	OMu48325
Clone ID Related Accession (Same CDS sequence)	XM_006516561.2 , XM_006516561.1
Accession Version	XM_006516561.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysosomal-trafficking regulator isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGAAGGTGAT AGACCAGAAG TTACAGAATC CATCAATCCT 
GGTGACAGAC TCATAGAAGA CGGCTGTATT CATTTGATTT CACTGGGGTC CAAAGCATTG 
ATGATCCAAG TGTGGGCTGA TCCCCACAGT GGCACTTTTA TCTTTCGTGT GTGCATGGAC 
TCAAATGATG ACACGAAAGC TGTCTCACTA GCACAGGTGG AATCACAGGA GAATATTTTC 
TTTCCAAGCA AATGGCAACA CTTAGTACTT ACCTATATTC AGCATCCTCA AGGGAAAAAG 
AATGTCCATG GGGAAATCTC CATATGGGTC TCTGGGCAGA GGAAGACTGA TGTCATCTTG 
GATTTTGTGC TCCCAAGAAA AACAAGCTTA TCATCAGACA GCAATAAAAC ATTTTGCATG 
ATTGGTCATT GCTTAACATC CCAAGAAGAG TCTCTGCAAT TAGCTGGAAA ATGGGACCTG 
GGGAACTTGC TCCTCTTCAA TGGAGCTAAA ATTGGCTCAC AAGAGGCCTT TTTCCTGTAT 
GCTTGTGGAC CCAACTACAC ATCCATCATG CCGTGTAAAT ATGGACAGCC AGTCATTGAC 
TACTCCAAAT ACATTAATAA AGACATTTTG AGATGTGATG AAATCAGAGA CCTTTTTATG 
ACCAAGAAAG AAGTGGATGT TGGTCTCTTA ATTGAAAGTC TTTCAGTTGT TTATACAACT 
TGCTGTCCTG CTCAGTACAC CATCTATGAA CCAGTGATTC GACTCAAGGG CCAAGTGAAA 
ACTCAGCCCT CTCAAAGACC CTTCAGCTCA AAGGAAGCCC AGAGCATCTT GCTAGAACCT 
TCTCAACTCA AAGGCCTCCA ACCTACGGAA TGTAAAGCCA TCCAGGGCAT TCTGCATGAG 
ATTGGTGGGG CTGGCACATT TGTTTTTCTC TTTGCTAGGG TTGTTGAACT TAGTAGCTGT 
GAAGAAACTC AAGCATTAGC ACTGCGGGTT ATACTGTCTT TAATTAAGTA CAGCCAACAG 
AGAACACAGG AACTGGAAAA TTGTAATGGA CTCTCTATGA TTCACCAAGT GTTGGTCAAA 
CAGAAATGCA TTGTTGGCTT TCACATTTTG AAGACCCTTC TTGAAGGTTG CTGCGGTGAA 
GAAGTTATCC ACGTCAGTGA GCATGGAGAG TTCAAGCTGG ATGTTGAGTC TCATGCTATA 
ATCCAAGATG TTAAGCTGCT GCAGGAACTG TTACTTGACT GGAAGATATG GAATAAGGCA 
GAGCAAGGTG TGTGGGAGAC TCTGCTAGCA GCTTTGGAAG TCCTCATCCG GGTAGAGCAC 
CACCAGCAGC AGTTTAATAT TAAGCAGTTG CTGAACGCCC ACGTGGTTCA CCACTTCCTA 
CTGACCTGTC AGGTTTTACA GGAACACAGA GAGGGGCAGC TTACATCTAT GCCCCGAGAA 
GTTTGTAGAT CATTTGTGAA AATCATTGCA GAAGTCCTTG GTTCTCCTCC AGACTTGGAA 
TTATTGACAG TTATTTTCAA TTTCCTGTTA GCTGTACACC CTCCTACTAA TACTTATGTT 
TGTCACAATC CCACAAACTT CTACTTCTCT TTGCACATAG ATGGCAAGAT CTTTCAGGAG 
AAAGTGCAGT CACTCGCGTA CCTGAGGCAT TCTAGCAGCG GAGGGCAAGC CTTTCCCAGC 
CCTGGATTCC TGGTAATAAG CCCATCTGCC TTTACTGCAG CTCCTCCTGA AGGAACCAGT 
TCTTCCAATA TTGTTCCACA GCGGATGGCT GCTCAGATGG TTCGATCTAG AAGTCTACCA 
GCATTTCCTA CTTATTTACC ACTAATACGA GCACAAAAAC TGGCTGCAAG TTTGGGTTTT 
AGTGTTGACA AGTTACAAAA TATTGCAGAT GCCAACCCAG AGAAACAGAA TCTTTTAGGA 
AGACCCTACG CACTGAAAAC AAGCAAAGAG GAAGCATTCA TCAGCAGCTG TGAGTCTGCA 
AAGACTGTTT GTGAAATGGA GGCTCTTCTT GGAGCCCACG CCTCTGCCAA TGGGGTTTCC 
AGAGGATCAC CGAGGTTCCC CAGGGCCAGA GTAGATCACA AAGATGTGGG AACAGAGCCC 
AGATCAGATG ATGACAGTCC TGGGGATGAG TCTTACCCAC GTCGGCCTGA CAACCTCAAG 
GGACTGGCCT CATTCCAGCG AAGCCAAAGC ACTGTCGCAA GCCTTGGGCT GGCGTTTCCC 
TCTCAGAATG GATCTGCAGT TGCTAGCAGG TGGCCAAGTC TTGTTGATAG GAATGCTGAT 
GACTGGGAGA ACTTTACCTT TTCTCCTGCT TATGAGGCAA GCTACAACCG AGCCACAAGC 
ACCCACAGTG TCATTGAAGA CTGTCTGATA CCTATCTGCT GTGGATTATA TGAACTCTTA 
AGTGGGGTTC TTCTTGTCCT GCCTGATGCT ATGCTTGAAG ATGTGATGGA CAGGATTATT 
CAAGCAGATA TTCTTCTAGT CCTTGTTAAC CACCCATCAC CTGCTATCCA GCAAGGAGTA 
ATTAAACTGT TACATGCATA CATTAATAGA GCATCAAAGG AGCAAAAGGA CAAGTTTCTG 
AAGAACCGTG GCTTTTCCTT ATTAGCCAAC CAGTTGTATC TTCATAGGGG AACTCAGGAG 
TTGTTGGAGT GCTTTGTTGA AATGTTCTTT GGTCGACCGA TTGGCCTGGA TGAAGAATTT 
GATCTGGAGG AAGTGAAGCA CATGGAACTG TTCCAGAAGT GGTCTGTCAT TCCCGTTCTC 
GGACTAATAG AGACCTCTCT CTATGACAAT GTCCTCTTGC ACAATGCTCT TTTACTTCTT 
CTGCAAGTTT TAAACTCTTG TTCCAAGGTA GCAGACATGC TACTGGACAA TGGTCTACTC 
TATGTATTAT GTAATACAGT AGCAGCCCTG AATGGATTAG AAAAGAACAT TCCTGTGAAC 
GAATACAAAT TGCTCGCATG TGATATACAG CAGCTTTTCA TAGCAGTTAC AATTCATGCT 
TGCAGTTCCT CAGGCACACA GTATTTTAGA GTGATTGAAG ACCTTATTGT ACTTCTTGGA 
TATCTTCATA ATAGCAAAAA CAAGAGGACA CAAAATATGG CTTTGGCCCT GCAGCTTAGA 
GTTCTCCAGG CTGCTTTGGA ATTTATAAGG AGCACAGCCA ATCATGACTC TGAAAGTCCA 
GTGCACTCGC CTTCTGCCCA CCGCCATTCA GTGCCTCCGA AGCGGAGAAG CATTGCTGGT 
TCAATTCCAG TGAAGGGTTC TGTTACTCCT TATCCACCAA GACGTCACTT TGGTTCCTAT 
GGTCAACTTC CGATGCCCTT CTTCAGTCCA TTAGATCAAG ACTCCCCTCA GCTCTCTCCT 
GGATGCACCA CATGTGCCGC ATCCCCCAGA GCAGGTTCTC GCAAATTCCC TCTGGCTCAG 
ACAGAGTCTC TGCTGATGAA GATGCGCTCA GTGGCCAGCG ATGAGCTACA CTCTATGATG 
CAGAGGAGGA TGAGCCAAGA GCACCCCAGC CAGGCCTCGG AGGCAGAGCT CGCTCAGAGG 
CTGCAGAGGC TCACCATCTT AGCTGTGAAC AGGATTATTT ACCAAGAGTT GAATTCAGAT 
ATTATTGACA TTTTGAGAAC TCCAGAAAAT ACATCCCAAA GCAAGACCTC AGTTTCTCAG 
ACTGAAATTT CTGAAGAAGA CATGCATCAT GAGCAACCTT CTGTATATAA TCCATTTCAA 
AAAGAAATGT TAACCTATCT GTTGGATGGC TTCAAAGTGT GTATTGGTTC AAGTAAAACT 
AGCGTTTCTA AGCAGCAGTG GACTAAAATC CTGGGGTCTT GTAAAGAAAC CCTCCGAGAC 
CAGCTTGGAA GATTGCTAGC GCATATTTTG TCTCCAACCC ACACTGTACA AGAACGGAAG 
CAGATACTTG AGATAGTTCA TGAACCAGCT CACCAGGATA TACTTCGTGA CTGTCTTAGC 
CCCTCCCCAC AACATGGAGC CAAGTTGGTT TTGTATTTGT CAGAGTTGAT ACATAATCAT 
CAGGATGAGT TAAGTGAAGA AGAAATGGAC ACAGCAGAAC TGCTTATGAA TGCTCTAAAG 
TTATGTGGCC ACAAGTGCAT CCCGCCCAGT GCCCCTTCCA AACCAGAGCT CATTAAGATC 
ATCAGAGAGG AGCAAAAGAA GTATGAAAGT GAAGAGAGTG TGAGCAAAGG CTCATGGCAG 
AAAACGGTGA ACAACAACCA GCAAAGTCTC TTCCAGAGGC TCGATTTCAA ATCCAAGGAT 
ATATCTAAAA TCGCTGCAGA CATCACCCAG GCTGTATCAC TCTCCCAAGG CATTGAAAGG 
AAGAAGGTGA TCCAGCACAT CAGAGGGATG TACAAAGTTG ACCTGAGTGC CAGCAGGCAC 
TGGCAGGAAT GCATCCAGCA GCTGACACAT GACAGAGCAG TCTGGTATGA CCCAATCTAC 
TATCCAACTT CATGGCAGTT GGATCCAACA GAAGGGCCAA ACCGAGAGAG GAGACGTTTG 
CAGAGATGCT ATCTAACTAT TCCCAATAAG TACCTCCTGA GGGACAGACA GAAGTCAGAA 
GGTGTGCTCA GGCCCCCACT CTCTTACCTT TTTGAAGATA AAACTCATTC TTCCTTCTCC 
TCTACTGTCA AAGACAAAGC TGCAAGTGAA TCCATCAGAG TGAATCGAAG ATGTATCAGT 
GTTGCACCAT CTAGAGAGAC AGCTGGGGAA TTGTTGTTAG GTAAATGTGG GATGTACTTT 
GTGGAAGACA ATGCCTCTGA CGCAGTTGAA AGCTCGAGCC TCCAAGGGGA GTTAGAGCCG 
GCATCATTTT CTTGGACATA TGAGGAAATT AAAGAAGTTC ACAGGCGCTG GTGGCAACTA 
AGAGATAATG CTGTAGAAAT CTTTTTAACA AATGGCAGAA CACTCCTATT AGCATTTGAC 
AATAACAAGG TTCGTGATGA CGTGTACCAG AGCATCCTCA CAAATAACCT CCCAAATCTT 
CTGGAGTACG GCAACATCAC CGCTCTGACA AACCTGTGGT ATTCTGGACA AATTACCAAT 
TTTGAATATT TGACTCATTT AAACAAGCAT GCGGGCCGGT CCTTCAATGA TCTCATGCAG 
TACCCGGTGT TCCCCTTCAT CCTTTCTGAC TATGTTAGTG AGACTCTTGA CCTCAATGAT 
CCATCTATCT ACAGAAACCT ATCTAAGCCT ATAGCTGTGC AGTATAAAGA AAAAGAAGAC 
CGTTACGTTG ACACATACAA GTACTTGGAG GAGGAGTATC GCAAGGGAGC TCGAGAGGAT 
GACCCCATGC CTCCTGTGCA ACCCTACCAC TATGGCTCCC ACTACTCCAA CAGCGGCACC 
GTGCTCCACT TCCTGGTCAG GATGCCGCCT TTCACTAAAA TGTTTCTAGC CTATCAAGAT 
CAGAGTTTCG ACATTCCAGA CCGAACATTT CATTCTACAA ACACAACTTG GCGCCTCTCC 
TCCTTTGAGT CCATGACTGA TGTGAAGGAG CTGATTCCAG AGTTTTTCTA TCTTCCTGAG 
TTCTTAGTGA ACCGTGAAGG CTTTGACTTC GGTGTTCGTC AGAATGGAGA GCGGGTTAAC 
CACGTCAATC TTCCTCCCTG GGCACGCAAC GATCCTCGGC TGTTCATCCT TATTCACCGG 
CAAGCACTAG AGTCTGACCA TGTGTCCCAG AACATCTGTC ACTGGATCGA CTTAGTGTTT 
GGCTACAAGC AAAAGGGGAA GGCGTCTGTT CAAGCCATCA ATGTCTTCCA CCCTGCTACA 
TATTTTGGAA TGGATGTCTC TGCAGTTGAA GATCCAGTGC AGAGACGGGC TTTAGAAACC 
ATGATAAAAA CCTACGGGCA GACCCCACGT CAGTTGTTCC ACACAGCCCA TGCCAGCCGA 
CCTGGAGCCA AGCTTAACAT CGAAGGAGAG CTTCCAGCAG CTGTTGGCTT GTTAGTCCAG 
TTCGCTTTCA GAGAGACCCG AGAACCAGTC AAGGAAGTCA CTCATCCGAG CCCTTTGTCA 
TGGATAAAAG GCTTGAAGTG GGGGGAGTAC GTAGGTTCCC CCAGTGCTCC AGTACCTGTG 
GTCTGCTTCA GCCAGCCCCA TGGAGAAAGA TTTGGTTCCC TGCAGGCACT GCCCACCAGA 
GCCATCTGTG GTTTATCACG AAACTTCTGT CTTCTGATGA CCTACAACAA GGAGCAAGGT 
GTGAGAAGCA TGAACAACAC CAATATTCAG TGGTCTGCTA TCCTAAGCTG GGGATATGCT 
GACAACATCT TACGGTTGAA AAGTAAGCAG AGTGAGCCAC CAATCAACTT CATTCAGAGT 
TCACAGCAGC ACCAGGTAAC CAGTTGTGCC TGGGTGCCTG ACAGTTGTCA GCTCTTCACT 
GGGAGCAAGT GTGGTGTCAT CACAGCCTAT ACCAACAGGC TCACCAGCAG CACGCCCTCA 
GAAATTGAAA TGGAGAGTCA GATGCATCTC TATGGACACA CAGAGGAGAT CACCGGCTTA 
TGTGTCTGCA AGCCGTACAG CGTGATGATA AGCGTGAGCA GAGACGGGAC CTGCATAGTA 
TGGGACCTGA ACAGGCTGTG CTATGTACAA AGTTTGGCTG GACACAAAAG CCCTGTGACG 
GCTGTCTCTG CCAGTGAAAC GTCAGGTGAC ATTGCTACTG TGTGTGACTC AGCTGGCGGG 
GGCAGTGACC TGAGACTCTG GACCGTGAAT GGGGACCTCG TTGGACATGT CCACTGCAGA 
GAGATCATTT GTTCTGTAGC TTTCTCCAAC CAGCCTGAGG GAGTCTCCAT CAACGTCATT 
GCTGGGGGAT TAGAAAATGG CATTGTAAGG CTATGGAGCA CATGGGACTT GAAGCCTGTG 
AGAGAGATTA CATTTCCCAA ATCAAATAAG CCCATCATAA GCCTGACATT CTCCTGTGAT 
GGCCACCATT TGTACACTGC CAACAGTGAG GGGACAGTGA TCGCATGGTG CCGGAAGGAC 
CAGCAGCGTG TGAAGCTGCC CATGTTCTAC TCTTTCCTCA GCAGCTACGC AGCTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516624.1
CDS	251..11770
Translation	MSTDSNSLAREFLIDVNQLCNAVVQRAEAREEEEEETHMATLGQYLVHGRGFLLLTKLNSIIDQALTCREELLTLLLSLLPLVWKIPVQEQQATDFNLPLSSDIILTKEKNSSLQKSTQGKLYLEGSAPSGQVSAKVNLFRKIRRQRKSTHRYSVRDARKTQLSTSDSEGNSDEKSTVVSKHRRLHALPRFLTQSPKEGHLVAKPDPSATKEQVLSDTMSVENSREVILRQDSNGDILSEPAALSILSNMNNSPFDLCHVLLSLLEKVCKFDIALNHNSSLALSVVPTLTEFLAGFGDCCNQSDTLEGQLVSAGWTEEPVALVQRMLFRTVLHLMSVDVSTAEAMPESLRKNLTELLRAALKIRACLEKQPEPFSPRQKKTLQEVQEGFVFSKYRHRALLLPELLEGVLQLLISCLQSAASNPFYFSQAMDLVQEFIQHQGFNLFETAVLQMEWLLSRDGVPSEAAEHLKALINSVIKIMSTVKKVKSEQLHHSMCTRKRHRRCEYSHFMQHHRDLSGLLVSAFKNQLSKSPFEETAEGDVQYPERCCCIAVCAHQCLRLLQQVSLSTTCVQILSGVHSVGICCCMDPKSVIVPLLHAFKLPALKAFQQHILNVLSKLLLDQLGGAELSPRIKKAACNICTVDSDQLAKLGETLQGTLCGAGPTSGLPSPSYRFQGILPSSGSEDLLWKWDALEAYQSFVFQEDRLHNIQIANHICNLIQKGNVVVQWKLYNYIFNPVLQRGVELVHHCQQLSIPSAQTHMCSQLKQYLPQEVLQIYLKTLPVLLKSRVIRDLFLSCNGVNQIIELNYLDGIRSHSLKAFETLIVSLGEQQKDAAVLDVDGLDIQQELPSLSVGPSLHKQQASSDSPCSLRKFYASLREPDPKKRKTIHQDVHINTINLFLCVAFLCVSKEADSDRESANESEDTSGYDSTASEPLSHMLPCLSLENVVLPSPECLHHAADIWSMCRWIYMLNSVFQKQFHRLGGFQVCHELIFMIIQKLFRSHTEDQGRRQGEMSRNENQELIRISYPELTLKGDVSSATAPDLGFLRKSADSVRGFQSQPVLPTSAEQIVATESVPGERKAFMSQQSETSLQSIRLLESLLAICLHSARACQQKMELELPSQGLSVENILCELREHLSQSKVAETELAKPLFDALLRVALGNHSADLGPGDAVTEKSHPSEEELLSQPGDFSEEAEDSQCCSLKLLGEEEGYEADSESNPEDVDTQDDGVELNPEAEGFSGSIVSNNLLENLTHGEIIYPEICMLGLNLLSASKAKLDVLAHVFESFLKIVRQKEKNISLLIQQGTVKILLGGFLNILTQTNSDFQACQRVLVDLLVSLMSSRTCSEDLTLLLRIFLEKSPCTEILLLGIHKIVESDFTMSPSQCLTFPFLHTPSLSNGVLSQKPPGILNSKALGLLRRARISRGKKEADRESFPYRLLSSWHIAPIHLPLLGQNCWPHLSEGFSVSLWFNVEYIHESESAAERGKRVKKRNKPSVLEDSSFEGAEGDRPEVTESINPGDRLIEDGCIHLISLGSKALMIQVWADPHSGTFIFRVCMDSNDDTKAVSLAQVESQENIFFPSKWQHLVLTYIQHPQGKKNVHGEISIWVSGQRKTDVILDFVLPRKTSLSSDSNKTFCMIGHCLTSQEESLQLAGKWDLGNLLLFNGAKIGSQEAFFLYACGPNYTSIMPCKYGQPVIDYSKYINKDILRCDEIRDLFMTKKEVDVGLLIESLSVVYTTCCPAQYTIYEPVIRLKGQVKTQPSQRPFSSKEAQSILLEPSQLKGLQPTECKAIQGILHEIGGAGTFVFLFARVVELSSCEETQALALRVILSLIKYSQQRTQELENCNGLSMIHQVLVKQKCIVGFHILKTLLEGCCGEEVIHVSEHGEFKLDVESHAIIQDVKLLQELLLDWKIWNKAEQGVWETLLAALEVLIRVEHHQQQFNIKQLLNAHVVHHFLLTCQVLQEHREGQLTSMPREVCRSFVKIIAEVLGSPPDLELLTVIFNFLLAVHPPTNTYVCHNPTNFYFSLHIDGKIFQEKVQSLAYLRHSSSGGQAFPSPGFLVISPSAFTAAPPEGTSSSNIVPQRMAAQMVRSRSLPAFPTYLPLIRAQKLAASLGFSVDKLQNIADANPEKQNLLGRPYALKTSKEEAFISSCESAKTVCEMEALLGAHASANGVSRGSPRFPRARVDHKDVGTEPRSDDDSPGDESYPRRPDNLKGLASFQRSQSTVASLGLAFPSQNGSAVASRWPSLVDRNADDWENFTFSPAYEASYNRATSTHSVIEDCLIPICCGLYELLSGVLLVLPDAMLEDVMDRIIQADILLVLVNHPSPAIQQGVIKLLHAYINRASKEQKDKFLKNRGFSLLANQLYLHRGTQELLECFVEMFFGRPIGLDEEFDLEEVKHMELFQKWSVIPVLGLIETSLYDNVLLHNALLLLLQVLNSCSKVADMLLDNGLLYVLCNTVAALNGLEKNIPVNEYKLLACDIQQLFIAVTIHACSSSGTQYFRVIEDLIVLLGYLHNSKNKRTQNMALALQLRVLQAALEFIRSTANHDSESPVHSPSAHRHSVPPKRRSIAGSIPVKGSVTPYPPRRHFGSYGQLPMPFFSPLDQDSPQLSPGCTTCAASPRAGSRKFPLAQTESLLMKMRSVASDELHSMMQRRMSQEHPSQASEAELAQRLQRLTILAVNRIIYQELNSDIIDILRTPENTSQSKTSVSQTEISEEDMHHEQPSVYNPFQKEMLTYLLDGFKVCIGSSKTSVSKQQWTKILGSCKETLRDQLGRLLAHILSPTHTVQERKQILEIVHEPAHQDILRDCLSPSPQHGAKLVLYLSELIHNHQDELSEEEMDTAELLMNALKLCGHKCIPPSAPSKPELIKIIREEQKKYESEESVSKGSWQKTVNNNQQSLFQRLDFKSKDISKIAADITQAVSLSQGIERKKVIQHIRGMYKVDLSASRHWQECIQQLTHDRAVWYDPIYYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLRDRQKSEGVLRPPLSYLFEDKTHSSFSSTVKDKAASESIRVNRRCISVAPSRETAGELLLGKCGMYFVEDNASDAVESSSLQGELEPASFSWTYEEIKEVHRRWWQLRDNAVEIFLTNGRTLLLAFDNNKVRDDVYQSILTNNLPNLLEYGNITALTNLWYSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILSDYVSETLDLNDPSIYRNLSKPIAVQYKEKEDRYVDTYKYLEEEYRKGAREDDPMPPVQPYHYGSHYSNSGTVLHFLVRMPPFTKMFLAYQDQSFDIPDRTFHSTNTTWRLSSFESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNGERVNHVNLPPWARNDPRLFILIHRQALESDHVSQNICHWIDLVFGYKQKGKASVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFHTAHASRPGAKLNIEGELPAAVGLLVQFAFRETREPVKEVTHPSPLSWIKGLKWGEYVGSPSAPVPVVCFSQPHGERFGSLQALPTRAICGLSRNFCLLMTYNKEQGVRSMNNTNIQWSAILSWGYADNILRLKSKQSEPPINFIQSSQQHQVTSCAWVPDSCQLFTGSKCGVITAYTNRLTSSTPSEIEMESQMHLYGHTEEITGLCVCKPYSVMISVSRDGTCIVWDLNRLCYVQSLAGHKSPVTAVSASETSGDIATVCDSAGGGSDLRLWTVNGDLVGHVHCREIICSVAFSNQPEGVSINVIAGGLENGIVRLWSTWDLKPVREITFPKSNKPIISLTFSCDGHHLYTANSEGTVIAWCRKDQQRVKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_006516561.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516561.1

ORF Insert Sequence:

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGAAGGTGAT AGACCAGAAG TTACAGAATC CATCAATCCT 
GGTGACAGAC TCATAGAAGA CGGCTGTATT CATTTGATTT CACTGGGGTC CAAAGCATTG 
ATGATCCAAG TGTGGGCTGA TCCCCACAGT GGCACTTTTA TCTTTCGTGT GTGCATGGAC 
TCAAATGATG ACACGAAAGC TGTCTCACTA GCACAGGTGG AATCACAGGA GAATATTTTC 
TTTCCAAGCA AATGGCAACA CTTAGTACTT ACCTATATTC AGCATCCTCA AGGGAAAAAG 
AATGTCCATG GGGAAATCTC CATATGGGTC TCTGGGCAGA GGAAGACTGA TGTCATCTTG 
GATTTTGTGC TCCCAAGAAA AACAAGCTTA TCATCAGACA GCAATAAAAC ATTTTGCATG 
ATTGGTCATT GCTTAACATC CCAAGAAGAG TCTCTGCAAT TAGCTGGAAA ATGGGACCTG 
GGGAACTTGC TCCTCTTCAA TGGAGCTAAA ATTGGCTCAC AAGAGGCCTT TTTCCTGTAT 
GCTTGTGGAC CCAACTACAC ATCCATCATG CCGTGTAAAT ATGGACAGCC AGTCATTGAC 
TACTCCAAAT ACATTAATAA AGACATTTTG AGATGTGATG AAATCAGAGA CCTTTTTATG 
ACCAAGAAAG AAGTGGATGT TGGTCTCTTA ATTGAAAGTC TTTCAGTTGT TTATACAACT 
TGCTGTCCTG CTCAGTACAC CATCTATGAA CCAGTGATTC GACTCAAGGG CCAAGTGAAA 
ACTCAGCCCT CTCAAAGACC CTTCAGCTCA AAGGAAGCCC AGAGCATCTT GCTAGAACCT 
TCTCAACTCA AAGGCCTCCA ACCTACGGAA TGTAAAGCCA TCCAGGGCAT TCTGCATGAG 
ATTGGTGGGG CTGGCACATT TGTTTTTCTC TTTGCTAGGG TTGTTGAACT TAGTAGCTGT 
GAAGAAACTC AAGCATTAGC ACTGCGGGTT ATACTGTCTT TAATTAAGTA CAGCCAACAG 
AGAACACAGG AACTGGAAAA TTGTAATGGA CTCTCTATGA TTCACCAAGT GTTGGTCAAA 
CAGAAATGCA TTGTTGGCTT TCACATTTTG AAGACCCTTC TTGAAGGTTG CTGCGGTGAA 
GAAGTTATCC ACGTCAGTGA GCATGGAGAG TTCAAGCTGG ATGTTGAGTC TCATGCTATA 
ATCCAAGATG TTAAGCTGCT GCAGGAACTG TTACTTGACT GGAAGATATG GAATAAGGCA 
GAGCAAGGTG TGTGGGAGAC TCTGCTAGCA GCTTTGGAAG TCCTCATCCG GGTAGAGCAC 
CACCAGCAGC AGTTTAATAT TAAGCAGTTG CTGAACGCCC ACGTGGTTCA CCACTTCCTA 
CTGACCTGTC AGGTTTTACA GGAACACAGA GAGGGGCAGC TTACATCTAT GCCCCGAGAA 
GTTTGTAGAT CATTTGTGAA AATCATTGCA GAAGTCCTTG GTTCTCCTCC AGACTTGGAA 
TTATTGACAG TTATTTTCAA TTTCCTGTTA GCTGTACACC CTCCTACTAA TACTTATGTT 
TGTCACAATC CCACAAACTT CTACTTCTCT TTGCACATAG ATGGCAAGAT CTTTCAGGAG 
AAAGTGCAGT CACTCGCGTA CCTGAGGCAT TCTAGCAGCG GAGGGCAAGC CTTTCCCAGC 
CCTGGATTCC TGGTAATAAG CCCATCTGCC TTTACTGCAG CTCCTCCTGA AGGAACCAGT 
TCTTCCAATA TTGTTCCACA GCGGATGGCT GCTCAGATGG TTCGATCTAG AAGTCTACCA 
GCATTTCCTA CTTATTTACC ACTAATACGA GCACAAAAAC TGGCTGCAAG TTTGGGTTTT 
AGTGTTGACA AGTTACAAAA TATTGCAGAT GCCAACCCAG AGAAACAGAA TCTTTTAGGA 
AGACCCTACG CACTGAAAAC AAGCAAAGAG GAAGCATTCA TCAGCAGCTG TGAGTCTGCA 
AAGACTGTTT GTGAAATGGA GGCTCTTCTT GGAGCCCACG CCTCTGCCAA TGGGGTTTCC 
AGAGGATCAC CGAGGTTCCC CAGGGCCAGA GTAGATCACA AAGATGTGGG AACAGAGCCC 
AGATCAGATG ATGACAGTCC TGGGGATGAG TCTTACCCAC GTCGGCCTGA CAACCTCAAG 
GGACTGGCCT CATTCCAGCG AAGCCAAAGC ACTGTCGCAA GCCTTGGGCT GGCGTTTCCC 
TCTCAGAATG GATCTGCAGT TGCTAGCAGG TGGCCAAGTC TTGTTGATAG GAATGCTGAT 
GACTGGGAGA ACTTTACCTT TTCTCCTGCT TATGAGGCAA GCTACAACCG AGCCACAAGC 
ACCCACAGTG TCATTGAAGA CTGTCTGATA CCTATCTGCT GTGGATTATA TGAACTCTTA 
AGTGGGGTTC TTCTTGTCCT GCCTGATGCT ATGCTTGAAG ATGTGATGGA CAGGATTATT 
CAAGCAGATA TTCTTCTAGT CCTTGTTAAC CACCCATCAC CTGCTATCCA GCAAGGAGTA 
ATTAAACTGT TACATGCATA CATTAATAGA GCATCAAAGG AGCAAAAGGA CAAGTTTCTG 
AAGAACCGTG GCTTTTCCTT ATTAGCCAAC CAGTTGTATC TTCATAGGGG AACTCAGGAG 
TTGTTGGAGT GCTTTGTTGA AATGTTCTTT GGTCGACCGA TTGGCCTGGA TGAAGAATTT 
GATCTGGAGG AAGTGAAGCA CATGGAACTG TTCCAGAAGT GGTCTGTCAT TCCCGTTCTC 
GGACTAATAG AGACCTCTCT CTATGACAAT GTCCTCTTGC ACAATGCTCT TTTACTTCTT 
CTGCAAGTTT TAAACTCTTG TTCCAAGGTA GCAGACATGC TACTGGACAA TGGTCTACTC 
TATGTATTAT GTAATACAGT AGCAGCCCTG AATGGATTAG AAAAGAACAT TCCTGTGAAC 
GAATACAAAT TGCTCGCATG TGATATACAG CAGCTTTTCA TAGCAGTTAC AATTCATGCT 
TGCAGTTCCT CAGGCACACA GTATTTTAGA GTGATTGAAG ACCTTATTGT ACTTCTTGGA 
TATCTTCATA ATAGCAAAAA CAAGAGGACA CAAAATATGG CTTTGGCCCT GCAGCTTAGA 
GTTCTCCAGG CTGCTTTGGA ATTTATAAGG AGCACAGCCA ATCATGACTC TGAAAGTCCA 
GTGCACTCGC CTTCTGCCCA CCGCCATTCA GTGCCTCCGA AGCGGAGAAG CATTGCTGGT 
TCAATTCCAG TGAAGGGTTC TGTTACTCCT TATCCACCAA GACGTCACTT TGGTTCCTAT 
GGTCAACTTC CGATGCCCTT CTTCAGTCCA TTAGATCAAG ACTCCCCTCA GCTCTCTCCT 
GGATGCACCA CATGTGCCGC ATCCCCCAGA GCAGGTTCTC GCAAATTCCC TCTGGCTCAG 
ACAGAGTCTC TGCTGATGAA GATGCGCTCA GTGGCCAGCG ATGAGCTACA CTCTATGATG 
CAGAGGAGGA TGAGCCAAGA GCACCCCAGC CAGGCCTCGG AGGCAGAGCT CGCTCAGAGG 
CTGCAGAGGC TCACCATCTT AGCTGTGAAC AGGATTATTT ACCAAGAGTT GAATTCAGAT 
ATTATTGACA TTTTGAGAAC TCCAGAAAAT ACATCCCAAA GCAAGACCTC AGTTTCTCAG 
ACTGAAATTT CTGAAGAAGA CATGCATCAT GAGCAACCTT CTGTATATAA TCCATTTCAA 
AAAGAAATGT TAACCTATCT GTTGGATGGC TTCAAAGTGT GTATTGGTTC AAGTAAAACT 
AGCGTTTCTA AGCAGCAGTG GACTAAAATC CTGGGGTCTT GTAAAGAAAC CCTCCGAGAC 
CAGCTTGGAA GATTGCTAGC GCATATTTTG TCTCCAACCC ACACTGTACA AGAACGGAAG 
CAGATACTTG AGATAGTTCA TGAACCAGCT CACCAGGATA TACTTCGTGA CTGTCTTAGC 
CCCTCCCCAC AACATGGAGC CAAGTTGGTT TTGTATTTGT CAGAGTTGAT ACATAATCAT 
CAGGATGAGT TAAGTGAAGA AGAAATGGAC ACAGCAGAAC TGCTTATGAA TGCTCTAAAG 
TTATGTGGCC ACAAGTGCAT CCCGCCCAGT GCCCCTTCCA AACCAGAGCT CATTAAGATC 
ATCAGAGAGG AGCAAAAGAA GTATGAAAGT GAAGAGAGTG TGAGCAAAGG CTCATGGCAG 
AAAACGGTGA ACAACAACCA GCAAAGTCTC TTCCAGAGGC TCGATTTCAA ATCCAAGGAT 
ATATCTAAAA TCGCTGCAGA CATCACCCAG GCTGTATCAC TCTCCCAAGG CATTGAAAGG 
AAGAAGGTGA TCCAGCACAT CAGAGGGATG TACAAAGTTG ACCTGAGTGC CAGCAGGCAC 
TGGCAGGAAT GCATCCAGCA GCTGACACAT GACAGAGCAG TCTGGTATGA CCCAATCTAC 
TATCCAACTT CATGGCAGTT GGATCCAACA GAAGGGCCAA ACCGAGAGAG GAGACGTTTG 
CAGAGATGCT ATCTAACTAT TCCCAATAAG TACCTCCTGA GGGACAGACA GAAGTCAGAA 
GGTGTGCTCA GGCCCCCACT CTCTTACCTT TTTGAAGATA AAACTCATTC TTCCTTCTCC 
TCTACTGTCA AAGACAAAGC TGCAAGTGAA TCCATCAGAG TGAATCGAAG ATGTATCAGT 
GTTGCACCAT CTAGAGAGAC AGCTGGGGAA TTGTTGTTAG GTAAATGTGG GATGTACTTT 
GTGGAAGACA ATGCCTCTGA CGCAGTTGAA AGCTCGAGCC TCCAAGGGGA GTTAGAGCCG 
GCATCATTTT CTTGGACATA TGAGGAAATT AAAGAAGTTC ACAGGCGCTG GTGGCAACTA 
AGAGATAATG CTGTAGAAAT CTTTTTAACA AATGGCAGAA CACTCCTATT AGCATTTGAC 
AATAACAAGG TTCGTGATGA CGTGTACCAG AGCATCCTCA CAAATAACCT CCCAAATCTT 
CTGGAGTACG GCAACATCAC CGCTCTGACA AACCTGTGGT ATTCTGGACA AATTACCAAT 
TTTGAATATT TGACTCATTT AAACAAGCAT GCGGGCCGGT CCTTCAATGA TCTCATGCAG 
TACCCGGTGT TCCCCTTCAT CCTTTCTGAC TATGTTAGTG AGACTCTTGA CCTCAATGAT 
CCATCTATCT ACAGAAACCT ATCTAAGCCT ATAGCTGTGC AGTATAAAGA AAAAGAAGAC 
CGTTACGTTG ACACATACAA GTACTTGGAG GAGGAGTATC GCAAGGGAGC TCGAGAGGAT 
GACCCCATGC CTCCTGTGCA ACCCTACCAC TATGGCTCCC ACTACTCCAA CAGCGGCACC 
GTGCTCCACT TCCTGGTCAG GATGCCGCCT TTCACTAAAA TGTTTCTAGC CTATCAAGAT 
CAGAGTTTCG ACATTCCAGA CCGAACATTT CATTCTACAA ACACAACTTG GCGCCTCTCC 
TCCTTTGAGT CCATGACTGA TGTGAAGGAG CTGATTCCAG AGTTTTTCTA TCTTCCTGAG 
TTCTTAGTGA ACCGTGAAGG CTTTGACTTC GGTGTTCGTC AGAATGGAGA GCGGGTTAAC 
CACGTCAATC TTCCTCCCTG GGCACGCAAC GATCCTCGGC TGTTCATCCT TATTCACCGG 
CAAGCACTAG AGTCTGACCA TGTGTCCCAG AACATCTGTC ACTGGATCGA CTTAGTGTTT 
GGCTACAAGC AAAAGGGGAA GGCGTCTGTT CAAGCCATCA ATGTCTTCCA CCCTGCTACA 
TATTTTGGAA TGGATGTCTC TGCAGTTGAA GATCCAGTGC AGAGACGGGC TTTAGAAACC 
ATGATAAAAA CCTACGGGCA GACCCCACGT CAGTTGTTCC ACACAGCCCA TGCCAGCCGA 
CCTGGAGCCA AGCTTAACAT CGAAGGAGAG CTTCCAGCAG CTGTTGGCTT GTTAGTCCAG 
TTCGCTTTCA GAGAGACCCG AGAACCAGTC AAGGAAGTCA CTCATCCGAG CCCTTTGTCA 
TGGATAAAAG GCTTGAAGTG GGGGGAGTAC GTAGGTTCCC CCAGTGCTCC AGTACCTGTG 
GTCTGCTTCA GCCAGCCCCA TGGAGAAAGA TTTGGTTCCC TGCAGGCACT GCCCACCAGA 
GCCATCTGTG GTTTATCACG AAACTTCTGT CTTCTGATGA CCTACAACAA GGAGCAAGGT 
GTGAGAAGCA TGAACAACAC CAATATTCAG TGGTCTGCTA TCCTAAGCTG GGGATATGCT 
GACAACATCT TACGGTTGAA AAGTAAGCAG AGTGAGCCAC CAATCAACTT CATTCAGAGT 
TCACAGCAGC ACCAGGTAAC CAGTTGTGCC TGGGTGCCTG ACAGTTGTCA GCTCTTCACT 
GGGAGCAAGT GTGGTGTCAT CACAGCCTAT ACCAACAGGC TCACCAGCAG CACGCCCTCA 
GAAATTGAAA TGGAGAGTCA GATGCATCTC TATGGACACA CAGAGGAGAT CACCGGCTTA 
TGTGTCTGCA AGCCGTACAG CGTGATGATA AGCGTGAGCA GAGACGGGAC CTGCATAGTA 
TGGGACCTGA ACAGGCTGTG CTATGTACAA AGTTTGGCTG GACACAAAAG CCCTGTGACG 
GCTGTCTCTG CCAGTGAAAC GTCAGGTGAC ATTGCTACTG TGTGTGACTC AGCTGGCGGG 
GGCAGTGACC TGAGACTCTG GACCGTGAAT GGGGACCTCG TTGGACATGT CCACTGCAGA 
GAGATCATTT GTTCTGTAGC TTTCTCCAAC CAGCCTGAGG GAGTCTCCAT CAACGTCATT 
GCTGGGGGAT TAGAAAATGG CATTGTAAGG CTATGGAGCA CATGGGACTT GAAGCCTGTG 
AGAGAGATTA CATTTCCCAA ATCAAATAAG CCCATCATAA GCCTGACATT CTCCTGTGAT 
GGCCACCATT TGTACACTGC CAACAGTGAG GGGACAGTGA TCGCATGGTG CCGGAAGGAC 
CAGCAGCGTG TGAAGCTGCC CATGTTCTAC TCTTTCCTCA GCAGCTACGC AGCTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lyst ( XM_006516562.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516625.1 Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X2, mRNA.

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CloneID	OMu48326
Clone ID Related Accession (Same CDS sequence)	XM_006516562.2 , XM_006516562.1
Accession Version	XM_006516562.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysosomal-trafficking regulator isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGGTGATAGA CCAGAAGTTA CAGAATCCAT CAATCCTGGT 
GACAGACTCA TAGAAGACGG CTGTATTCAT TTGATTTCAC TGGGGTCCAA AGCATTGATG 
ATCCAAGTGT GGGCTGATCC CCACAGTGGC ACTTTTATCT TTCGTGTGTG CATGGACTCA 
AATGATGACA CGAAAGCTGT CTCACTAGCA CAGGTGGAAT CACAGGAGAA TATTTTCTTT 
CCAAGCAAAT GGCAACACTT AGTACTTACC TATATTCAGC ATCCTCAAGG GAAAAAGAAT 
GTCCATGGGG AAATCTCCAT ATGGGTCTCT GGGCAGAGGA AGACTGATGT CATCTTGGAT 
TTTGTGCTCC CAAGAAAAAC AAGCTTATCA TCAGACAGCA ATAAAACATT TTGCATGATT 
GGTCATTGCT TAACATCCCA AGAAGAGTCT CTGCAATTAG CTGGAAAATG GGACCTGGGG 
AACTTGCTCC TCTTCAATGG AGCTAAAATT GGCTCACAAG AGGCCTTTTT CCTGTATGCT 
TGTGGACCCA ACTACACATC CATCATGCCG TGTAAATATG GACAGCCAGT CATTGACTAC 
TCCAAATACA TTAATAAAGA CATTTTGAGA TGTGATGAAA TCAGAGACCT TTTTATGACC 
AAGAAAGAAG TGGATGTTGG TCTCTTAATT GAAAGTCTTT CAGTTGTTTA TACAACTTGC 
TGTCCTGCTC AGTACACCAT CTATGAACCA GTGATTCGAC TCAAGGGCCA AGTGAAAACT 
CAGCCCTCTC AAAGACCCTT CAGCTCAAAG GAAGCCCAGA GCATCTTGCT AGAACCTTCT 
CAACTCAAAG GCCTCCAACC TACGGAATGT AAAGCCATCC AGGGCATTCT GCATGAGATT 
GGTGGGGCTG GCACATTTGT TTTTCTCTTT GCTAGGGTTG TTGAACTTAG TAGCTGTGAA 
GAAACTCAAG CATTAGCACT GCGGGTTATA CTGTCTTTAA TTAAGTACAG CCAACAGAGA 
ACACAGGAAC TGGAAAATTG TAATGGACTC TCTATGATTC ACCAAGTGTT GGTCAAACAG 
AAATGCATTG TTGGCTTTCA CATTTTGAAG ACCCTTCTTG AAGGTTGCTG CGGTGAAGAA 
GTTATCCACG TCAGTGAGCA TGGAGAGTTC AAGCTGGATG TTGAGTCTCA TGCTATAATC 
CAAGATGTTA AGCTGCTGCA GGAACTGTTA CTTGACTGGA AGATATGGAA TAAGGCAGAG 
CAAGGTGTGT GGGAGACTCT GCTAGCAGCT TTGGAAGTCC TCATCCGGGT AGAGCACCAC 
CAGCAGCAGT TTAATATTAA GCAGTTGCTG AACGCCCACG TGGTTCACCA CTTCCTACTG 
ACCTGTCAGG TTTTACAGGA ACACAGAGAG GGGCAGCTTA CATCTATGCC CCGAGAAGTT 
TGTAGATCAT TTGTGAAAAT CATTGCAGAA GTCCTTGGTT CTCCTCCAGA CTTGGAATTA 
TTGACAGTTA TTTTCAATTT CCTGTTAGCT GTACACCCTC CTACTAATAC TTATGTTTGT 
CACAATCCCA CAAACTTCTA CTTCTCTTTG CACATAGATG GCAAGATCTT TCAGGAGAAA 
GTGCAGTCAC TCGCGTACCT GAGGCATTCT AGCAGCGGAG GGCAAGCCTT TCCCAGCCCT 
GGATTCCTGG TAATAAGCCC ATCTGCCTTT ACTGCAGCTC CTCCTGAAGG AACCAGTTCT 
TCCAATATTG TTCCACAGCG GATGGCTGCT CAGATGGTTC GATCTAGAAG TCTACCAGCA 
TTTCCTACTT ATTTACCACT AATACGAGCA CAAAAACTGG CTGCAAGTTT GGGTTTTAGT 
GTTGACAAGT TACAAAATAT TGCAGATGCC AACCCAGAGA AACAGAATCT TTTAGGAAGA 
CCCTACGCAC TGAAAACAAG CAAAGAGGAA GCATTCATCA GCAGCTGTGA GTCTGCAAAG 
ACTGTTTGTG AAATGGAGGC TCTTCTTGGA GCCCACGCCT CTGCCAATGG GGTTTCCAGA 
GGATCACCGA GGTTCCCCAG GGCCAGAGTA GATCACAAAG ATGTGGGAAC AGAGCCCAGA 
TCAGATGATG ACAGTCCTGG GGATGAGTCT TACCCACGTC GGCCTGACAA CCTCAAGGGA 
CTGGCCTCAT TCCAGCGAAG CCAAAGCACT GTCGCAAGCC TTGGGCTGGC GTTTCCCTCT 
CAGAATGGAT CTGCAGTTGC TAGCAGGTGG CCAAGTCTTG TTGATAGGAA TGCTGATGAC 
TGGGAGAACT TTACCTTTTC TCCTGCTTAT GAGGCAAGCT ACAACCGAGC CACAAGCACC 
CACAGTGTCA TTGAAGACTG TCTGATACCT ATCTGCTGTG GATTATATGA ACTCTTAAGT 
GGGGTTCTTC TTGTCCTGCC TGATGCTATG CTTGAAGATG TGATGGACAG GATTATTCAA 
GCAGATATTC TTCTAGTCCT TGTTAACCAC CCATCACCTG CTATCCAGCA AGGAGTAATT 
AAACTGTTAC ATGCATACAT TAATAGAGCA TCAAAGGAGC AAAAGGACAA GTTTCTGAAG 
AACCGTGGCT TTTCCTTATT AGCCAACCAG TTGTATCTTC ATAGGGGAAC TCAGGAGTTG 
TTGGAGTGCT TTGTTGAAAT GTTCTTTGGT CGACCGATTG GCCTGGATGA AGAATTTGAT 
CTGGAGGAAG TGAAGCACAT GGAACTGTTC CAGAAGTGGT CTGTCATTCC CGTTCTCGGA 
CTAATAGAGA CCTCTCTCTA TGACAATGTC CTCTTGCACA ATGCTCTTTT ACTTCTTCTG 
CAAGTTTTAA ACTCTTGTTC CAAGGTAGCA GACATGCTAC TGGACAATGG TCTACTCTAT 
GTATTATGTA ATACAGTAGC AGCCCTGAAT GGATTAGAAA AGAACATTCC TGTGAACGAA 
TACAAATTGC TCGCATGTGA TATACAGCAG CTTTTCATAG CAGTTACAAT TCATGCTTGC 
AGTTCCTCAG GCACACAGTA TTTTAGAGTG ATTGAAGACC TTATTGTACT TCTTGGATAT 
CTTCATAATA GCAAAAACAA GAGGACACAA AATATGGCTT TGGCCCTGCA GCTTAGAGTT 
CTCCAGGCTG CTTTGGAATT TATAAGGAGC ACAGCCAATC ATGACTCTGA AAGTCCAGTG 
CACTCGCCTT CTGCCCACCG CCATTCAGTG CCTCCGAAGC GGAGAAGCAT TGCTGGTTCA 
ATTCCAGTGA AGGGTTCTGT TACTCCTTAT CCACCAAGAC GTCACTTTGG TTCCTATGGT 
CAACTTCCGA TGCCCTTCTT CAGTCCATTA GATCAAGACT CCCCTCAGCT CTCTCCTGGA 
TGCACCACAT GTGCCGCATC CCCCAGAGCA GGTTCTCGCA AATTCCCTCT GGCTCAGACA 
GAGTCTCTGC TGATGAAGAT GCGCTCAGTG GCCAGCGATG AGCTACACTC TATGATGCAG 
AGGAGGATGA GCCAAGAGCA CCCCAGCCAG GCCTCGGAGG CAGAGCTCGC TCAGAGGCTG 
CAGAGGCTCA CCATCTTAGC TGTGAACAGG ATTATTTACC AAGAGTTGAA TTCAGATATT 
ATTGACATTT TGAGAACTCC AGAAAATACA TCCCAAAGCA AGACCTCAGT TTCTCAGACT 
GAAATTTCTG AAGAAGACAT GCATCATGAG CAACCTTCTG TATATAATCC ATTTCAAAAA 
GAAATGTTAA CCTATCTGTT GGATGGCTTC AAAGTGTGTA TTGGTTCAAG TAAAACTAGC 
GTTTCTAAGC AGCAGTGGAC TAAAATCCTG GGGTCTTGTA AAGAAACCCT CCGAGACCAG 
CTTGGAAGAT TGCTAGCGCA TATTTTGTCT CCAACCCACA CTGTACAAGA ACGGAAGCAG 
ATACTTGAGA TAGTTCATGA ACCAGCTCAC CAGGATATAC TTCGTGACTG TCTTAGCCCC 
TCCCCACAAC ATGGAGCCAA GTTGGTTTTG TATTTGTCAG AGTTGATACA TAATCATCAG 
GATGAGTTAA GTGAAGAAGA AATGGACACA GCAGAACTGC TTATGAATGC TCTAAAGTTA 
TGTGGCCACA AGTGCATCCC GCCCAGTGCC CCTTCCAAAC CAGAGCTCAT TAAGATCATC 
AGAGAGGAGC AAAAGAAGTA TGAAAGTGAA GAGAGTGTGA GCAAAGGCTC ATGGCAGAAA 
ACGGTGAACA ACAACCAGCA AAGTCTCTTC CAGAGGCTCG ATTTCAAATC CAAGGATATA 
TCTAAAATCG CTGCAGACAT CACCCAGGCT GTATCACTCT CCCAAGGCAT TGAAAGGAAG 
AAGGTGATCC AGCACATCAG AGGGATGTAC AAAGTTGACC TGAGTGCCAG CAGGCACTGG 
CAGGAATGCA TCCAGCAGCT GACACATGAC AGAGCAGTCT GGTATGACCC AATCTACTAT 
CCAACTTCAT GGCAGTTGGA TCCAACAGAA GGGCCAAACC GAGAGAGGAG ACGTTTGCAG 
AGATGCTATC TAACTATTCC CAATAAGTAC CTCCTGAGGG ACAGACAGAA GTCAGAAGGT 
GTGCTCAGGC CCCCACTCTC TTACCTTTTT GAAGATAAAA CTCATTCTTC CTTCTCCTCT 
ACTGTCAAAG ACAAAGCTGC AAGTGAATCC ATCAGAGTGA ATCGAAGATG TATCAGTGTT 
GCACCATCTA GAGAGACAGC TGGGGAATTG TTGTTAGGTA AATGTGGGAT GTACTTTGTG 
GAAGACAATG CCTCTGACGC AGTTGAAAGC TCGAGCCTCC AAGGGGAGTT AGAGCCGGCA 
TCATTTTCTT GGACATATGA GGAAATTAAA GAAGTTCACA GGCGCTGGTG GCAACTAAGA 
GATAATGCTG TAGAAATCTT TTTAACAAAT GGCAGAACAC TCCTATTAGC ATTTGACAAT 
AACAAGGTTC GTGATGACGT GTACCAGAGC ATCCTCACAA ATAACCTCCC AAATCTTCTG 
GAGTACGGCA ACATCACCGC TCTGACAAAC CTGTGGTATT CTGGACAAAT TACCAATTTT 
GAATATTTGA CTCATTTAAA CAAGCATGCG GGCCGGTCCT TCAATGATCT CATGCAGTAC 
CCGGTGTTCC CCTTCATCCT TTCTGACTAT GTTAGTGAGA CTCTTGACCT CAATGATCCA 
TCTATCTACA GAAACCTATC TAAGCCTATA GCTGTGCAGT ATAAAGAAAA AGAAGACCGT 
TACGTTGACA CATACAAGTA CTTGGAGGAG GAGTATCGCA AGGGAGCTCG AGAGGATGAC 
CCCATGCCTC CTGTGCAACC CTACCACTAT GGCTCCCACT ACTCCAACAG CGGCACCGTG 
CTCCACTTCC TGGTCAGGAT GCCGCCTTTC ACTAAAATGT TTCTAGCCTA TCAAGATCAG 
AGTTTCGACA TTCCAGACCG AACATTTCAT TCTACAAACA CAACTTGGCG CCTCTCCTCC 
TTTGAGTCCA TGACTGATGT GAAGGAGCTG ATTCCAGAGT TTTTCTATCT TCCTGAGTTC 
TTAGTGAACC GTGAAGGCTT TGACTTCGGT GTTCGTCAGA ATGGAGAGCG GGTTAACCAC 
GTCAATCTTC CTCCCTGGGC ACGCAACGAT CCTCGGCTGT TCATCCTTAT TCACCGGCAA 
GCACTAGAGT CTGACCATGT GTCCCAGAAC ATCTGTCACT GGATCGACTT AGTGTTTGGC 
TACAAGCAAA AGGGGAAGGC GTCTGTTCAA GCCATCAATG TCTTCCACCC TGCTACATAT 
TTTGGAATGG ATGTCTCTGC AGTTGAAGAT CCAGTGCAGA GACGGGCTTT AGAAACCATG 
ATAAAAACCT ACGGGCAGAC CCCACGTCAG TTGTTCCACA CAGCCCATGC CAGCCGACCT 
GGAGCCAAGC TTAACATCGA AGGAGAGCTT CCAGCAGCTG TTGGCTTGTT AGTCCAGTTC 
GCTTTCAGAG AGACCCGAGA ACCAGTCAAG GAAGTCACTC ATCCGAGCCC TTTGTCATGG 
ATAAAAGGCT TGAAGTGGGG GGAGTACGTA GGTTCCCCCA GTGCTCCAGT ACCTGTGGTC 
TGCTTCAGCC AGCCCCATGG AGAAAGATTT GGTTCCCTGC AGGCACTGCC CACCAGAGCC 
ATCTGTGGTT TATCACGAAA CTTCTGTCTT CTGATGACCT ACAACAAGGA GCAAGGTGTG 
AGAAGCATGA ACAACACCAA TATTCAGTGG TCTGCTATCC TAAGCTGGGG ATATGCTGAC 
AACATCTTAC GGTTGAAAAG TAAGCAGAGT GAGCCACCAA TCAACTTCAT TCAGAGTTCA 
CAGCAGCACC AGGTAACCAG TTGTGCCTGG GTGCCTGACA GTTGTCAGCT CTTCACTGGG 
AGCAAGTGTG GTGTCATCAC AGCCTATACC AACAGGCTCA CCAGCAGCAC GCCCTCAGAA 
ATTGAAATGG AGAGTCAGAT GCATCTCTAT GGACACACAG AGGAGATCAC CGGCTTATGT 
GTCTGCAAGC CGTACAGCGT GATGATAAGC GTGAGCAGAG ACGGGACCTG CATAGTATGG 
GACCTGAACA GGCTGTGCTA TGTACAAAGT TTGGCTGGAC ACAAAAGCCC TGTGACGGCT 
GTCTCTGCCA GTGAAACGTC AGGTGACATT GCTACTGTGT GTGACTCAGC TGGCGGGGGC 
AGTGACCTGA GACTCTGGAC CGTGAATGGG GACCTCGTTG GACATGTCCA CTGCAGAGAG 
ATCATTTGTT CTGTAGCTTT CTCCAACCAG CCTGAGGGAG TCTCCATCAA CGTCATTGCT 
GGGGGATTAG AAAATGGCAT TGTAAGGCTA TGGAGCACAT GGGACTTGAA GCCTGTGAGA 
GAGATTACAT TTCCCAAATC AAATAAGCCC ATCATAAGCC TGACATTCTC CTGTGATGGC 
CACCATTTGT ACACTGCCAA CAGTGAGGGG ACAGTGATCG CATGGTGCCG GAAGGACCAG 
CAGCGTGTGA AGCTGCCCAT GTTCTACTCT TTCCTCAGCA GCTACGCAGC TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516625.1
CDS	280..11796
Translation	MSTDSNSLAREFLIDVNQLCNAVVQRAEAREEEEEETHMATLGQYLVHGRGFLLLTKLNSIIDQALTCREELLTLLLSLLPLVWKIPVQEQQATDFNLPLSSDIILTKEKNSSLQKSTQGKLYLEGSAPSGQVSAKVNLFRKIRRQRKSTHRYSVRDARKTQLSTSDSEGNSDEKSTVVSKHRRLHALPRFLTQSPKEGHLVAKPDPSATKEQVLSDTMSVENSREVILRQDSNGDILSEPAALSILSNMNNSPFDLCHVLLSLLEKVCKFDIALNHNSSLALSVVPTLTEFLAGFGDCCNQSDTLEGQLVSAGWTEEPVALVQRMLFRTVLHLMSVDVSTAEAMPESLRKNLTELLRAALKIRACLEKQPEPFSPRQKKTLQEVQEGFVFSKYRHRALLLPELLEGVLQLLISCLQSAASNPFYFSQAMDLVQEFIQHQGFNLFETAVLQMEWLLSRDGVPSEAAEHLKALINSVIKIMSTVKKVKSEQLHHSMCTRKRHRRCEYSHFMQHHRDLSGLLVSAFKNQLSKSPFEETAEGDVQYPERCCCIAVCAHQCLRLLQQVSLSTTCVQILSGVHSVGICCCMDPKSVIVPLLHAFKLPALKAFQQHILNVLSKLLLDQLGGAELSPRIKKAACNICTVDSDQLAKLGETLQGTLCGAGPTSGLPSPSYRFQGILPSSGSEDLLWKWDALEAYQSFVFQEDRLHNIQIANHICNLIQKGNVVVQWKLYNYIFNPVLQRGVELVHHCQQLSIPSAQTHMCSQLKQYLPQEVLQIYLKTLPVLLKSRVIRDLFLSCNGVNQIIELNYLDGIRSHSLKAFETLIVSLGEQQKDAAVLDVDGLDIQQELPSLSVGPSLHKQQASSDSPCSLRKFYASLREPDPKKRKTIHQDVHINTINLFLCVAFLCVSKEADSDRESANESEDTSGYDSTASEPLSHMLPCLSLENVVLPSPECLHHAADIWSMCRWIYMLNSVFQKQFHRLGGFQVCHELIFMIIQKLFRSHTEDQGRRQGEMSRNENQELIRISYPELTLKGDVSSATAPDLGFLRKSADSVRGFQSQPVLPTSAEQIVATESVPGERKAFMSQQSETSLQSIRLLESLLAICLHSARACQQKMELELPSQGLSVENILCELREHLSQSKVAETELAKPLFDALLRVALGNHSADLGPGDAVTEKSHPSEEELLSQPGDFSEEAEDSQCCSLKLLGEEEGYEADSESNPEDVDTQDDGVELNPEAEGFSGSIVSNNLLENLTHGEIIYPEICMLGLNLLSASKAKLDVLAHVFESFLKIVRQKEKNISLLIQQGTVKILLGGFLNILTQTNSDFQACQRVLVDLLVSLMSSRTCSEDLTLLLRIFLEKSPCTEILLLGIHKIVESDFTMSPSQCLTFPFLHTPSLSNGVLSQKPPGILNSKALGLLRRARISRGKKEADRESFPYRLLSSWHIAPIHLPLLGQNCWPHLSEGFSVSLWFNVEYIHESESAAERGKRVKKRNKPSVLEDSSFEGAGDRPEVTESINPGDRLIEDGCIHLISLGSKALMIQVWADPHSGTFIFRVCMDSNDDTKAVSLAQVESQENIFFPSKWQHLVLTYIQHPQGKKNVHGEISIWVSGQRKTDVILDFVLPRKTSLSSDSNKTFCMIGHCLTSQEESLQLAGKWDLGNLLLFNGAKIGSQEAFFLYACGPNYTSIMPCKYGQPVIDYSKYINKDILRCDEIRDLFMTKKEVDVGLLIESLSVVYTTCCPAQYTIYEPVIRLKGQVKTQPSQRPFSSKEAQSILLEPSQLKGLQPTECKAIQGILHEIGGAGTFVFLFARVVELSSCEETQALALRVILSLIKYSQQRTQELENCNGLSMIHQVLVKQKCIVGFHILKTLLEGCCGEEVIHVSEHGEFKLDVESHAIIQDVKLLQELLLDWKIWNKAEQGVWETLLAALEVLIRVEHHQQQFNIKQLLNAHVVHHFLLTCQVLQEHREGQLTSMPREVCRSFVKIIAEVLGSPPDLELLTVIFNFLLAVHPPTNTYVCHNPTNFYFSLHIDGKIFQEKVQSLAYLRHSSSGGQAFPSPGFLVISPSAFTAAPPEGTSSSNIVPQRMAAQMVRSRSLPAFPTYLPLIRAQKLAASLGFSVDKLQNIADANPEKQNLLGRPYALKTSKEEAFISSCESAKTVCEMEALLGAHASANGVSRGSPRFPRARVDHKDVGTEPRSDDDSPGDESYPRRPDNLKGLASFQRSQSTVASLGLAFPSQNGSAVASRWPSLVDRNADDWENFTFSPAYEASYNRATSTHSVIEDCLIPICCGLYELLSGVLLVLPDAMLEDVMDRIIQADILLVLVNHPSPAIQQGVIKLLHAYINRASKEQKDKFLKNRGFSLLANQLYLHRGTQELLECFVEMFFGRPIGLDEEFDLEEVKHMELFQKWSVIPVLGLIETSLYDNVLLHNALLLLLQVLNSCSKVADMLLDNGLLYVLCNTVAALNGLEKNIPVNEYKLLACDIQQLFIAVTIHACSSSGTQYFRVIEDLIVLLGYLHNSKNKRTQNMALALQLRVLQAALEFIRSTANHDSESPVHSPSAHRHSVPPKRRSIAGSIPVKGSVTPYPPRRHFGSYGQLPMPFFSPLDQDSPQLSPGCTTCAASPRAGSRKFPLAQTESLLMKMRSVASDELHSMMQRRMSQEHPSQASEAELAQRLQRLTILAVNRIIYQELNSDIIDILRTPENTSQSKTSVSQTEISEEDMHHEQPSVYNPFQKEMLTYLLDGFKVCIGSSKTSVSKQQWTKILGSCKETLRDQLGRLLAHILSPTHTVQERKQILEIVHEPAHQDILRDCLSPSPQHGAKLVLYLSELIHNHQDELSEEEMDTAELLMNALKLCGHKCIPPSAPSKPELIKIIREEQKKYESEESVSKGSWQKTVNNNQQSLFQRLDFKSKDISKIAADITQAVSLSQGIERKKVIQHIRGMYKVDLSASRHWQECIQQLTHDRAVWYDPIYYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLRDRQKSEGVLRPPLSYLFEDKTHSSFSSTVKDKAASESIRVNRRCISVAPSRETAGELLLGKCGMYFVEDNASDAVESSSLQGELEPASFSWTYEEIKEVHRRWWQLRDNAVEIFLTNGRTLLLAFDNNKVRDDVYQSILTNNLPNLLEYGNITALTNLWYSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILSDYVSETLDLNDPSIYRNLSKPIAVQYKEKEDRYVDTYKYLEEEYRKGAREDDPMPPVQPYHYGSHYSNSGTVLHFLVRMPPFTKMFLAYQDQSFDIPDRTFHSTNTTWRLSSFESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNGERVNHVNLPPWARNDPRLFILIHRQALESDHVSQNICHWIDLVFGYKQKGKASVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFHTAHASRPGAKLNIEGELPAAVGLLVQFAFRETREPVKEVTHPSPLSWIKGLKWGEYVGSPSAPVPVVCFSQPHGERFGSLQALPTRAICGLSRNFCLLMTYNKEQGVRSMNNTNIQWSAILSWGYADNILRLKSKQSEPPINFIQSSQQHQVTSCAWVPDSCQLFTGSKCGVITAYTNRLTSSTPSEIEMESQMHLYGHTEEITGLCVCKPYSVMISVSRDGTCIVWDLNRLCYVQSLAGHKSPVTAVSASETSGDIATVCDSAGGGSDLRLWTVNGDLVGHVHCREIICSVAFSNQPEGVSINVIAGGLENGIVRLWSTWDLKPVREITFPKSNKPIISLTFSCDGHHLYTANSEGTVIAWCRKDQQRVKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_006516562.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516562.2

ORF Insert Sequence:

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGGTGATAGA CCAGAAGTTA CAGAATCCAT CAATCCTGGT 
GACAGACTCA TAGAAGACGG CTGTATTCAT TTGATTTCAC TGGGGTCCAA AGCATTGATG 
ATCCAAGTGT GGGCTGATCC CCACAGTGGC ACTTTTATCT TTCGTGTGTG CATGGACTCA 
AATGATGACA CGAAAGCTGT CTCACTAGCA CAGGTGGAAT CACAGGAGAA TATTTTCTTT 
CCAAGCAAAT GGCAACACTT AGTACTTACC TATATTCAGC ATCCTCAAGG GAAAAAGAAT 
GTCCATGGGG AAATCTCCAT ATGGGTCTCT GGGCAGAGGA AGACTGATGT CATCTTGGAT 
TTTGTGCTCC CAAGAAAAAC AAGCTTATCA TCAGACAGCA ATAAAACATT TTGCATGATT 
GGTCATTGCT TAACATCCCA AGAAGAGTCT CTGCAATTAG CTGGAAAATG GGACCTGGGG 
AACTTGCTCC TCTTCAATGG AGCTAAAATT GGCTCACAAG AGGCCTTTTT CCTGTATGCT 
TGTGGACCCA ACTACACATC CATCATGCCG TGTAAATATG GACAGCCAGT CATTGACTAC 
TCCAAATACA TTAATAAAGA CATTTTGAGA TGTGATGAAA TCAGAGACCT TTTTATGACC 
AAGAAAGAAG TGGATGTTGG TCTCTTAATT GAAAGTCTTT CAGTTGTTTA TACAACTTGC 
TGTCCTGCTC AGTACACCAT CTATGAACCA GTGATTCGAC TCAAGGGCCA AGTGAAAACT 
CAGCCCTCTC AAAGACCCTT CAGCTCAAAG GAAGCCCAGA GCATCTTGCT AGAACCTTCT 
CAACTCAAAG GCCTCCAACC TACGGAATGT AAAGCCATCC AGGGCATTCT GCATGAGATT 
GGTGGGGCTG GCACATTTGT TTTTCTCTTT GCTAGGGTTG TTGAACTTAG TAGCTGTGAA 
GAAACTCAAG CATTAGCACT GCGGGTTATA CTGTCTTTAA TTAAGTACAG CCAACAGAGA 
ACACAGGAAC TGGAAAATTG TAATGGACTC TCTATGATTC ACCAAGTGTT GGTCAAACAG 
AAATGCATTG TTGGCTTTCA CATTTTGAAG ACCCTTCTTG AAGGTTGCTG CGGTGAAGAA 
GTTATCCACG TCAGTGAGCA TGGAGAGTTC AAGCTGGATG TTGAGTCTCA TGCTATAATC 
CAAGATGTTA AGCTGCTGCA GGAACTGTTA CTTGACTGGA AGATATGGAA TAAGGCAGAG 
CAAGGTGTGT GGGAGACTCT GCTAGCAGCT TTGGAAGTCC TCATCCGGGT AGAGCACCAC 
CAGCAGCAGT TTAATATTAA GCAGTTGCTG AACGCCCACG TGGTTCACCA CTTCCTACTG 
ACCTGTCAGG TTTTACAGGA ACACAGAGAG GGGCAGCTTA CATCTATGCC CCGAGAAGTT 
TGTAGATCAT TTGTGAAAAT CATTGCAGAA GTCCTTGGTT CTCCTCCAGA CTTGGAATTA 
TTGACAGTTA TTTTCAATTT CCTGTTAGCT GTACACCCTC CTACTAATAC TTATGTTTGT 
CACAATCCCA CAAACTTCTA CTTCTCTTTG CACATAGATG GCAAGATCTT TCAGGAGAAA 
GTGCAGTCAC TCGCGTACCT GAGGCATTCT AGCAGCGGAG GGCAAGCCTT TCCCAGCCCT 
GGATTCCTGG TAATAAGCCC ATCTGCCTTT ACTGCAGCTC CTCCTGAAGG AACCAGTTCT 
TCCAATATTG TTCCACAGCG GATGGCTGCT CAGATGGTTC GATCTAGAAG TCTACCAGCA 
TTTCCTACTT ATTTACCACT AATACGAGCA CAAAAACTGG CTGCAAGTTT GGGTTTTAGT 
GTTGACAAGT TACAAAATAT TGCAGATGCC AACCCAGAGA AACAGAATCT TTTAGGAAGA 
CCCTACGCAC TGAAAACAAG CAAAGAGGAA GCATTCATCA GCAGCTGTGA GTCTGCAAAG 
ACTGTTTGTG AAATGGAGGC TCTTCTTGGA GCCCACGCCT CTGCCAATGG GGTTTCCAGA 
GGATCACCGA GGTTCCCCAG GGCCAGAGTA GATCACAAAG ATGTGGGAAC AGAGCCCAGA 
TCAGATGATG ACAGTCCTGG GGATGAGTCT TACCCACGTC GGCCTGACAA CCTCAAGGGA 
CTGGCCTCAT TCCAGCGAAG CCAAAGCACT GTCGCAAGCC TTGGGCTGGC GTTTCCCTCT 
CAGAATGGAT CTGCAGTTGC TAGCAGGTGG CCAAGTCTTG TTGATAGGAA TGCTGATGAC 
TGGGAGAACT TTACCTTTTC TCCTGCTTAT GAGGCAAGCT ACAACCGAGC CACAAGCACC 
CACAGTGTCA TTGAAGACTG TCTGATACCT ATCTGCTGTG GATTATATGA ACTCTTAAGT 
GGGGTTCTTC TTGTCCTGCC TGATGCTATG CTTGAAGATG TGATGGACAG GATTATTCAA 
GCAGATATTC TTCTAGTCCT TGTTAACCAC CCATCACCTG CTATCCAGCA AGGAGTAATT 
AAACTGTTAC ATGCATACAT TAATAGAGCA TCAAAGGAGC AAAAGGACAA GTTTCTGAAG 
AACCGTGGCT TTTCCTTATT AGCCAACCAG TTGTATCTTC ATAGGGGAAC TCAGGAGTTG 
TTGGAGTGCT TTGTTGAAAT GTTCTTTGGT CGACCGATTG GCCTGGATGA AGAATTTGAT 
CTGGAGGAAG TGAAGCACAT GGAACTGTTC CAGAAGTGGT CTGTCATTCC CGTTCTCGGA 
CTAATAGAGA CCTCTCTCTA TGACAATGTC CTCTTGCACA ATGCTCTTTT ACTTCTTCTG 
CAAGTTTTAA ACTCTTGTTC CAAGGTAGCA GACATGCTAC TGGACAATGG TCTACTCTAT 
GTATTATGTA ATACAGTAGC AGCCCTGAAT GGATTAGAAA AGAACATTCC TGTGAACGAA 
TACAAATTGC TCGCATGTGA TATACAGCAG CTTTTCATAG CAGTTACAAT TCATGCTTGC 
AGTTCCTCAG GCACACAGTA TTTTAGAGTG ATTGAAGACC TTATTGTACT TCTTGGATAT 
CTTCATAATA GCAAAAACAA GAGGACACAA AATATGGCTT TGGCCCTGCA GCTTAGAGTT 
CTCCAGGCTG CTTTGGAATT TATAAGGAGC ACAGCCAATC ATGACTCTGA AAGTCCAGTG 
CACTCGCCTT CTGCCCACCG CCATTCAGTG CCTCCGAAGC GGAGAAGCAT TGCTGGTTCA 
ATTCCAGTGA AGGGTTCTGT TACTCCTTAT CCACCAAGAC GTCACTTTGG TTCCTATGGT 
CAACTTCCGA TGCCCTTCTT CAGTCCATTA GATCAAGACT CCCCTCAGCT CTCTCCTGGA 
TGCACCACAT GTGCCGCATC CCCCAGAGCA GGTTCTCGCA AATTCCCTCT GGCTCAGACA 
GAGTCTCTGC TGATGAAGAT GCGCTCAGTG GCCAGCGATG AGCTACACTC TATGATGCAG 
AGGAGGATGA GCCAAGAGCA CCCCAGCCAG GCCTCGGAGG CAGAGCTCGC TCAGAGGCTG 
CAGAGGCTCA CCATCTTAGC TGTGAACAGG ATTATTTACC AAGAGTTGAA TTCAGATATT 
ATTGACATTT TGAGAACTCC AGAAAATACA TCCCAAAGCA AGACCTCAGT TTCTCAGACT 
GAAATTTCTG AAGAAGACAT GCATCATGAG CAACCTTCTG TATATAATCC ATTTCAAAAA 
GAAATGTTAA CCTATCTGTT GGATGGCTTC AAAGTGTGTA TTGGTTCAAG TAAAACTAGC 
GTTTCTAAGC AGCAGTGGAC TAAAATCCTG GGGTCTTGTA AAGAAACCCT CCGAGACCAG 
CTTGGAAGAT TGCTAGCGCA TATTTTGTCT CCAACCCACA CTGTACAAGA ACGGAAGCAG 
ATACTTGAGA TAGTTCATGA ACCAGCTCAC CAGGATATAC TTCGTGACTG TCTTAGCCCC 
TCCCCACAAC ATGGAGCCAA GTTGGTTTTG TATTTGTCAG AGTTGATACA TAATCATCAG 
GATGAGTTAA GTGAAGAAGA AATGGACACA GCAGAACTGC TTATGAATGC TCTAAAGTTA 
TGTGGCCACA AGTGCATCCC GCCCAGTGCC CCTTCCAAAC CAGAGCTCAT TAAGATCATC 
AGAGAGGAGC AAAAGAAGTA TGAAAGTGAA GAGAGTGTGA GCAAAGGCTC ATGGCAGAAA 
ACGGTGAACA ACAACCAGCA AAGTCTCTTC CAGAGGCTCG ATTTCAAATC CAAGGATATA 
TCTAAAATCG CTGCAGACAT CACCCAGGCT GTATCACTCT CCCAAGGCAT TGAAAGGAAG 
AAGGTGATCC AGCACATCAG AGGGATGTAC AAAGTTGACC TGAGTGCCAG CAGGCACTGG 
CAGGAATGCA TCCAGCAGCT GACACATGAC AGAGCAGTCT GGTATGACCC AATCTACTAT 
CCAACTTCAT GGCAGTTGGA TCCAACAGAA GGGCCAAACC GAGAGAGGAG ACGTTTGCAG 
AGATGCTATC TAACTATTCC CAATAAGTAC CTCCTGAGGG ACAGACAGAA GTCAGAAGGT 
GTGCTCAGGC CCCCACTCTC TTACCTTTTT GAAGATAAAA CTCATTCTTC CTTCTCCTCT 
ACTGTCAAAG ACAAAGCTGC AAGTGAATCC ATCAGAGTGA ATCGAAGATG TATCAGTGTT 
GCACCATCTA GAGAGACAGC TGGGGAATTG TTGTTAGGTA AATGTGGGAT GTACTTTGTG 
GAAGACAATG CCTCTGACGC AGTTGAAAGC TCGAGCCTCC AAGGGGAGTT AGAGCCGGCA 
TCATTTTCTT GGACATATGA GGAAATTAAA GAAGTTCACA GGCGCTGGTG GCAACTAAGA 
GATAATGCTG TAGAAATCTT TTTAACAAAT GGCAGAACAC TCCTATTAGC ATTTGACAAT 
AACAAGGTTC GTGATGACGT GTACCAGAGC ATCCTCACAA ATAACCTCCC AAATCTTCTG 
GAGTACGGCA ACATCACCGC TCTGACAAAC CTGTGGTATT CTGGACAAAT TACCAATTTT 
GAATATTTGA CTCATTTAAA CAAGCATGCG GGCCGGTCCT TCAATGATCT CATGCAGTAC 
CCGGTGTTCC CCTTCATCCT TTCTGACTAT GTTAGTGAGA CTCTTGACCT CAATGATCCA 
TCTATCTACA GAAACCTATC TAAGCCTATA GCTGTGCAGT ATAAAGAAAA AGAAGACCGT 
TACGTTGACA CATACAAGTA CTTGGAGGAG GAGTATCGCA AGGGAGCTCG AGAGGATGAC 
CCCATGCCTC CTGTGCAACC CTACCACTAT GGCTCCCACT ACTCCAACAG CGGCACCGTG 
CTCCACTTCC TGGTCAGGAT GCCGCCTTTC ACTAAAATGT TTCTAGCCTA TCAAGATCAG 
AGTTTCGACA TTCCAGACCG AACATTTCAT TCTACAAACA CAACTTGGCG CCTCTCCTCC 
TTTGAGTCCA TGACTGATGT GAAGGAGCTG ATTCCAGAGT TTTTCTATCT TCCTGAGTTC 
TTAGTGAACC GTGAAGGCTT TGACTTCGGT GTTCGTCAGA ATGGAGAGCG GGTTAACCAC 
GTCAATCTTC CTCCCTGGGC ACGCAACGAT CCTCGGCTGT TCATCCTTAT TCACCGGCAA 
GCACTAGAGT CTGACCATGT GTCCCAGAAC ATCTGTCACT GGATCGACTT AGTGTTTGGC 
TACAAGCAAA AGGGGAAGGC GTCTGTTCAA GCCATCAATG TCTTCCACCC TGCTACATAT 
TTTGGAATGG ATGTCTCTGC AGTTGAAGAT CCAGTGCAGA GACGGGCTTT AGAAACCATG 
ATAAAAACCT ACGGGCAGAC CCCACGTCAG TTGTTCCACA CAGCCCATGC CAGCCGACCT 
GGAGCCAAGC TTAACATCGA AGGAGAGCTT CCAGCAGCTG TTGGCTTGTT AGTCCAGTTC 
GCTTTCAGAG AGACCCGAGA ACCAGTCAAG GAAGTCACTC ATCCGAGCCC TTTGTCATGG 
ATAAAAGGCT TGAAGTGGGG GGAGTACGTA GGTTCCCCCA GTGCTCCAGT ACCTGTGGTC 
TGCTTCAGCC AGCCCCATGG AGAAAGATTT GGTTCCCTGC AGGCACTGCC CACCAGAGCC 
ATCTGTGGTT TATCACGAAA CTTCTGTCTT CTGATGACCT ACAACAAGGA GCAAGGTGTG 
AGAAGCATGA ACAACACCAA TATTCAGTGG TCTGCTATCC TAAGCTGGGG ATATGCTGAC 
AACATCTTAC GGTTGAAAAG TAAGCAGAGT GAGCCACCAA TCAACTTCAT TCAGAGTTCA 
CAGCAGCACC AGGTAACCAG TTGTGCCTGG GTGCCTGACA GTTGTCAGCT CTTCACTGGG 
AGCAAGTGTG GTGTCATCAC AGCCTATACC AACAGGCTCA CCAGCAGCAC GCCCTCAGAA 
ATTGAAATGG AGAGTCAGAT GCATCTCTAT GGACACACAG AGGAGATCAC CGGCTTATGT 
GTCTGCAAGC CGTACAGCGT GATGATAAGC GTGAGCAGAG ACGGGACCTG CATAGTATGG 
GACCTGAACA GGCTGTGCTA TGTACAAAGT TTGGCTGGAC ACAAAAGCCC TGTGACGGCT 
GTCTCTGCCA GTGAAACGTC AGGTGACATT GCTACTGTGT GTGACTCAGC TGGCGGGGGC 
AGTGACCTGA GACTCTGGAC CGTGAATGGG GACCTCGTTG GACATGTCCA CTGCAGAGAG 
ATCATTTGTT CTGTAGCTTT CTCCAACCAG CCTGAGGGAG TCTCCATCAA CGTCATTGCT 
GGGGGATTAG AAAATGGCAT TGTAAGGCTA TGGAGCACAT GGGACTTGAA GCCTGTGAGA 
GAGATTACAT TTCCCAAATC AAATAAGCCC ATCATAAGCC TGACATTCTC CTGTGATGGC 
CACCATTTGT ACACTGCCAA CAGTGAGGGG ACAGTGATCG CATGGTGCCG GAAGGACCAG 
CAGCGTGTGA AGCTGCCCAT GTTCTACTCT TTCCTCAGCA GCTACGCAGC TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lyst ( XM_006516562.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516625.1 Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X2, mRNA.

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CloneID	OMu48326
Clone ID Related Accession (Same CDS sequence)	XM_006516562.2 , XM_006516562.1
Accession Version	XM_006516562.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysosomal-trafficking regulator isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGGTGATAGA CCAGAAGTTA CAGAATCCAT CAATCCTGGT 
GACAGACTCA TAGAAGACGG CTGTATTCAT TTGATTTCAC TGGGGTCCAA AGCATTGATG 
ATCCAAGTGT GGGCTGATCC CCACAGTGGC ACTTTTATCT TTCGTGTGTG CATGGACTCA 
AATGATGACA CGAAAGCTGT CTCACTAGCA CAGGTGGAAT CACAGGAGAA TATTTTCTTT 
CCAAGCAAAT GGCAACACTT AGTACTTACC TATATTCAGC ATCCTCAAGG GAAAAAGAAT 
GTCCATGGGG AAATCTCCAT ATGGGTCTCT GGGCAGAGGA AGACTGATGT CATCTTGGAT 
TTTGTGCTCC CAAGAAAAAC AAGCTTATCA TCAGACAGCA ATAAAACATT TTGCATGATT 
GGTCATTGCT TAACATCCCA AGAAGAGTCT CTGCAATTAG CTGGAAAATG GGACCTGGGG 
AACTTGCTCC TCTTCAATGG AGCTAAAATT GGCTCACAAG AGGCCTTTTT CCTGTATGCT 
TGTGGACCCA ACTACACATC CATCATGCCG TGTAAATATG GACAGCCAGT CATTGACTAC 
TCCAAATACA TTAATAAAGA CATTTTGAGA TGTGATGAAA TCAGAGACCT TTTTATGACC 
AAGAAAGAAG TGGATGTTGG TCTCTTAATT GAAAGTCTTT CAGTTGTTTA TACAACTTGC 
TGTCCTGCTC AGTACACCAT CTATGAACCA GTGATTCGAC TCAAGGGCCA AGTGAAAACT 
CAGCCCTCTC AAAGACCCTT CAGCTCAAAG GAAGCCCAGA GCATCTTGCT AGAACCTTCT 
CAACTCAAAG GCCTCCAACC TACGGAATGT AAAGCCATCC AGGGCATTCT GCATGAGATT 
GGTGGGGCTG GCACATTTGT TTTTCTCTTT GCTAGGGTTG TTGAACTTAG TAGCTGTGAA 
GAAACTCAAG CATTAGCACT GCGGGTTATA CTGTCTTTAA TTAAGTACAG CCAACAGAGA 
ACACAGGAAC TGGAAAATTG TAATGGACTC TCTATGATTC ACCAAGTGTT GGTCAAACAG 
AAATGCATTG TTGGCTTTCA CATTTTGAAG ACCCTTCTTG AAGGTTGCTG CGGTGAAGAA 
GTTATCCACG TCAGTGAGCA TGGAGAGTTC AAGCTGGATG TTGAGTCTCA TGCTATAATC 
CAAGATGTTA AGCTGCTGCA GGAACTGTTA CTTGACTGGA AGATATGGAA TAAGGCAGAG 
CAAGGTGTGT GGGAGACTCT GCTAGCAGCT TTGGAAGTCC TCATCCGGGT AGAGCACCAC 
CAGCAGCAGT TTAATATTAA GCAGTTGCTG AACGCCCACG TGGTTCACCA CTTCCTACTG 
ACCTGTCAGG TTTTACAGGA ACACAGAGAG GGGCAGCTTA CATCTATGCC CCGAGAAGTT 
TGTAGATCAT TTGTGAAAAT CATTGCAGAA GTCCTTGGTT CTCCTCCAGA CTTGGAATTA 
TTGACAGTTA TTTTCAATTT CCTGTTAGCT GTACACCCTC CTACTAATAC TTATGTTTGT 
CACAATCCCA CAAACTTCTA CTTCTCTTTG CACATAGATG GCAAGATCTT TCAGGAGAAA 
GTGCAGTCAC TCGCGTACCT GAGGCATTCT AGCAGCGGAG GGCAAGCCTT TCCCAGCCCT 
GGATTCCTGG TAATAAGCCC ATCTGCCTTT ACTGCAGCTC CTCCTGAAGG AACCAGTTCT 
TCCAATATTG TTCCACAGCG GATGGCTGCT CAGATGGTTC GATCTAGAAG TCTACCAGCA 
TTTCCTACTT ATTTACCACT AATACGAGCA CAAAAACTGG CTGCAAGTTT GGGTTTTAGT 
GTTGACAAGT TACAAAATAT TGCAGATGCC AACCCAGAGA AACAGAATCT TTTAGGAAGA 
CCCTACGCAC TGAAAACAAG CAAAGAGGAA GCATTCATCA GCAGCTGTGA GTCTGCAAAG 
ACTGTTTGTG AAATGGAGGC TCTTCTTGGA GCCCACGCCT CTGCCAATGG GGTTTCCAGA 
GGATCACCGA GGTTCCCCAG GGCCAGAGTA GATCACAAAG ATGTGGGAAC AGAGCCCAGA 
TCAGATGATG ACAGTCCTGG GGATGAGTCT TACCCACGTC GGCCTGACAA CCTCAAGGGA 
CTGGCCTCAT TCCAGCGAAG CCAAAGCACT GTCGCAAGCC TTGGGCTGGC GTTTCCCTCT 
CAGAATGGAT CTGCAGTTGC TAGCAGGTGG CCAAGTCTTG TTGATAGGAA TGCTGATGAC 
TGGGAGAACT TTACCTTTTC TCCTGCTTAT GAGGCAAGCT ACAACCGAGC CACAAGCACC 
CACAGTGTCA TTGAAGACTG TCTGATACCT ATCTGCTGTG GATTATATGA ACTCTTAAGT 
GGGGTTCTTC TTGTCCTGCC TGATGCTATG CTTGAAGATG TGATGGACAG GATTATTCAA 
GCAGATATTC TTCTAGTCCT TGTTAACCAC CCATCACCTG CTATCCAGCA AGGAGTAATT 
AAACTGTTAC ATGCATACAT TAATAGAGCA TCAAAGGAGC AAAAGGACAA GTTTCTGAAG 
AACCGTGGCT TTTCCTTATT AGCCAACCAG TTGTATCTTC ATAGGGGAAC TCAGGAGTTG 
TTGGAGTGCT TTGTTGAAAT GTTCTTTGGT CGACCGATTG GCCTGGATGA AGAATTTGAT 
CTGGAGGAAG TGAAGCACAT GGAACTGTTC CAGAAGTGGT CTGTCATTCC CGTTCTCGGA 
CTAATAGAGA CCTCTCTCTA TGACAATGTC CTCTTGCACA ATGCTCTTTT ACTTCTTCTG 
CAAGTTTTAA ACTCTTGTTC CAAGGTAGCA GACATGCTAC TGGACAATGG TCTACTCTAT 
GTATTATGTA ATACAGTAGC AGCCCTGAAT GGATTAGAAA AGAACATTCC TGTGAACGAA 
TACAAATTGC TCGCATGTGA TATACAGCAG CTTTTCATAG CAGTTACAAT TCATGCTTGC 
AGTTCCTCAG GCACACAGTA TTTTAGAGTG ATTGAAGACC TTATTGTACT TCTTGGATAT 
CTTCATAATA GCAAAAACAA GAGGACACAA AATATGGCTT TGGCCCTGCA GCTTAGAGTT 
CTCCAGGCTG CTTTGGAATT TATAAGGAGC ACAGCCAATC ATGACTCTGA AAGTCCAGTG 
CACTCGCCTT CTGCCCACCG CCATTCAGTG CCTCCGAAGC GGAGAAGCAT TGCTGGTTCA 
ATTCCAGTGA AGGGTTCTGT TACTCCTTAT CCACCAAGAC GTCACTTTGG TTCCTATGGT 
CAACTTCCGA TGCCCTTCTT CAGTCCATTA GATCAAGACT CCCCTCAGCT CTCTCCTGGA 
TGCACCACAT GTGCCGCATC CCCCAGAGCA GGTTCTCGCA AATTCCCTCT GGCTCAGACA 
GAGTCTCTGC TGATGAAGAT GCGCTCAGTG GCCAGCGATG AGCTACACTC TATGATGCAG 
AGGAGGATGA GCCAAGAGCA CCCCAGCCAG GCCTCGGAGG CAGAGCTCGC TCAGAGGCTG 
CAGAGGCTCA CCATCTTAGC TGTGAACAGG ATTATTTACC AAGAGTTGAA TTCAGATATT 
ATTGACATTT TGAGAACTCC AGAAAATACA TCCCAAAGCA AGACCTCAGT TTCTCAGACT 
GAAATTTCTG AAGAAGACAT GCATCATGAG CAACCTTCTG TATATAATCC ATTTCAAAAA 
GAAATGTTAA CCTATCTGTT GGATGGCTTC AAAGTGTGTA TTGGTTCAAG TAAAACTAGC 
GTTTCTAAGC AGCAGTGGAC TAAAATCCTG GGGTCTTGTA AAGAAACCCT CCGAGACCAG 
CTTGGAAGAT TGCTAGCGCA TATTTTGTCT CCAACCCACA CTGTACAAGA ACGGAAGCAG 
ATACTTGAGA TAGTTCATGA ACCAGCTCAC CAGGATATAC TTCGTGACTG TCTTAGCCCC 
TCCCCACAAC ATGGAGCCAA GTTGGTTTTG TATTTGTCAG AGTTGATACA TAATCATCAG 
GATGAGTTAA GTGAAGAAGA AATGGACACA GCAGAACTGC TTATGAATGC TCTAAAGTTA 
TGTGGCCACA AGTGCATCCC GCCCAGTGCC CCTTCCAAAC CAGAGCTCAT TAAGATCATC 
AGAGAGGAGC AAAAGAAGTA TGAAAGTGAA GAGAGTGTGA GCAAAGGCTC ATGGCAGAAA 
ACGGTGAACA ACAACCAGCA AAGTCTCTTC CAGAGGCTCG ATTTCAAATC CAAGGATATA 
TCTAAAATCG CTGCAGACAT CACCCAGGCT GTATCACTCT CCCAAGGCAT TGAAAGGAAG 
AAGGTGATCC AGCACATCAG AGGGATGTAC AAAGTTGACC TGAGTGCCAG CAGGCACTGG 
CAGGAATGCA TCCAGCAGCT GACACATGAC AGAGCAGTCT GGTATGACCC AATCTACTAT 
CCAACTTCAT GGCAGTTGGA TCCAACAGAA GGGCCAAACC GAGAGAGGAG ACGTTTGCAG 
AGATGCTATC TAACTATTCC CAATAAGTAC CTCCTGAGGG ACAGACAGAA GTCAGAAGGT 
GTGCTCAGGC CCCCACTCTC TTACCTTTTT GAAGATAAAA CTCATTCTTC CTTCTCCTCT 
ACTGTCAAAG ACAAAGCTGC AAGTGAATCC ATCAGAGTGA ATCGAAGATG TATCAGTGTT 
GCACCATCTA GAGAGACAGC TGGGGAATTG TTGTTAGGTA AATGTGGGAT GTACTTTGTG 
GAAGACAATG CCTCTGACGC AGTTGAAAGC TCGAGCCTCC AAGGGGAGTT AGAGCCGGCA 
TCATTTTCTT GGACATATGA GGAAATTAAA GAAGTTCACA GGCGCTGGTG GCAACTAAGA 
GATAATGCTG TAGAAATCTT TTTAACAAAT GGCAGAACAC TCCTATTAGC ATTTGACAAT 
AACAAGGTTC GTGATGACGT GTACCAGAGC ATCCTCACAA ATAACCTCCC AAATCTTCTG 
GAGTACGGCA ACATCACCGC TCTGACAAAC CTGTGGTATT CTGGACAAAT TACCAATTTT 
GAATATTTGA CTCATTTAAA CAAGCATGCG GGCCGGTCCT TCAATGATCT CATGCAGTAC 
CCGGTGTTCC CCTTCATCCT TTCTGACTAT GTTAGTGAGA CTCTTGACCT CAATGATCCA 
TCTATCTACA GAAACCTATC TAAGCCTATA GCTGTGCAGT ATAAAGAAAA AGAAGACCGT 
TACGTTGACA CATACAAGTA CTTGGAGGAG GAGTATCGCA AGGGAGCTCG AGAGGATGAC 
CCCATGCCTC CTGTGCAACC CTACCACTAT GGCTCCCACT ACTCCAACAG CGGCACCGTG 
CTCCACTTCC TGGTCAGGAT GCCGCCTTTC ACTAAAATGT TTCTAGCCTA TCAAGATCAG 
AGTTTCGACA TTCCAGACCG AACATTTCAT TCTACAAACA CAACTTGGCG CCTCTCCTCC 
TTTGAGTCCA TGACTGATGT GAAGGAGCTG ATTCCAGAGT TTTTCTATCT TCCTGAGTTC 
TTAGTGAACC GTGAAGGCTT TGACTTCGGT GTTCGTCAGA ATGGAGAGCG GGTTAACCAC 
GTCAATCTTC CTCCCTGGGC ACGCAACGAT CCTCGGCTGT TCATCCTTAT TCACCGGCAA 
GCACTAGAGT CTGACCATGT GTCCCAGAAC ATCTGTCACT GGATCGACTT AGTGTTTGGC 
TACAAGCAAA AGGGGAAGGC GTCTGTTCAA GCCATCAATG TCTTCCACCC TGCTACATAT 
TTTGGAATGG ATGTCTCTGC AGTTGAAGAT CCAGTGCAGA GACGGGCTTT AGAAACCATG 
ATAAAAACCT ACGGGCAGAC CCCACGTCAG TTGTTCCACA CAGCCCATGC CAGCCGACCT 
GGAGCCAAGC TTAACATCGA AGGAGAGCTT CCAGCAGCTG TTGGCTTGTT AGTCCAGTTC 
GCTTTCAGAG AGACCCGAGA ACCAGTCAAG GAAGTCACTC ATCCGAGCCC TTTGTCATGG 
ATAAAAGGCT TGAAGTGGGG GGAGTACGTA GGTTCCCCCA GTGCTCCAGT ACCTGTGGTC 
TGCTTCAGCC AGCCCCATGG AGAAAGATTT GGTTCCCTGC AGGCACTGCC CACCAGAGCC 
ATCTGTGGTT TATCACGAAA CTTCTGTCTT CTGATGACCT ACAACAAGGA GCAAGGTGTG 
AGAAGCATGA ACAACACCAA TATTCAGTGG TCTGCTATCC TAAGCTGGGG ATATGCTGAC 
AACATCTTAC GGTTGAAAAG TAAGCAGAGT GAGCCACCAA TCAACTTCAT TCAGAGTTCA 
CAGCAGCACC AGGTAACCAG TTGTGCCTGG GTGCCTGACA GTTGTCAGCT CTTCACTGGG 
AGCAAGTGTG GTGTCATCAC AGCCTATACC AACAGGCTCA CCAGCAGCAC GCCCTCAGAA 
ATTGAAATGG AGAGTCAGAT GCATCTCTAT GGACACACAG AGGAGATCAC CGGCTTATGT 
GTCTGCAAGC CGTACAGCGT GATGATAAGC GTGAGCAGAG ACGGGACCTG CATAGTATGG 
GACCTGAACA GGCTGTGCTA TGTACAAAGT TTGGCTGGAC ACAAAAGCCC TGTGACGGCT 
GTCTCTGCCA GTGAAACGTC AGGTGACATT GCTACTGTGT GTGACTCAGC TGGCGGGGGC 
AGTGACCTGA GACTCTGGAC CGTGAATGGG GACCTCGTTG GACATGTCCA CTGCAGAGAG 
ATCATTTGTT CTGTAGCTTT CTCCAACCAG CCTGAGGGAG TCTCCATCAA CGTCATTGCT 
GGGGGATTAG AAAATGGCAT TGTAAGGCTA TGGAGCACAT GGGACTTGAA GCCTGTGAGA 
GAGATTACAT TTCCCAAATC AAATAAGCCC ATCATAAGCC TGACATTCTC CTGTGATGGC 
CACCATTTGT ACACTGCCAA CAGTGAGGGG ACAGTGATCG CATGGTGCCG GAAGGACCAG 
CAGCGTGTGA AGCTGCCCAT GTTCTACTCT TTCCTCAGCA GCTACGCAGC TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516625.1
CDS	251..11767
Translation	MSTDSNSLAREFLIDVNQLCNAVVQRAEAREEEEEETHMATLGQYLVHGRGFLLLTKLNSIIDQALTCREELLTLLLSLLPLVWKIPVQEQQATDFNLPLSSDIILTKEKNSSLQKSTQGKLYLEGSAPSGQVSAKVNLFRKIRRQRKSTHRYSVRDARKTQLSTSDSEGNSDEKSTVVSKHRRLHALPRFLTQSPKEGHLVAKPDPSATKEQVLSDTMSVENSREVILRQDSNGDILSEPAALSILSNMNNSPFDLCHVLLSLLEKVCKFDIALNHNSSLALSVVPTLTEFLAGFGDCCNQSDTLEGQLVSAGWTEEPVALVQRMLFRTVLHLMSVDVSTAEAMPESLRKNLTELLRAALKIRACLEKQPEPFSPRQKKTLQEVQEGFVFSKYRHRALLLPELLEGVLQLLISCLQSAASNPFYFSQAMDLVQEFIQHQGFNLFETAVLQMEWLLSRDGVPSEAAEHLKALINSVIKIMSTVKKVKSEQLHHSMCTRKRHRRCEYSHFMQHHRDLSGLLVSAFKNQLSKSPFEETAEGDVQYPERCCCIAVCAHQCLRLLQQVSLSTTCVQILSGVHSVGICCCMDPKSVIVPLLHAFKLPALKAFQQHILNVLSKLLLDQLGGAELSPRIKKAACNICTVDSDQLAKLGETLQGTLCGAGPTSGLPSPSYRFQGILPSSGSEDLLWKWDALEAYQSFVFQEDRLHNIQIANHICNLIQKGNVVVQWKLYNYIFNPVLQRGVELVHHCQQLSIPSAQTHMCSQLKQYLPQEVLQIYLKTLPVLLKSRVIRDLFLSCNGVNQIIELNYLDGIRSHSLKAFETLIVSLGEQQKDAAVLDVDGLDIQQELPSLSVGPSLHKQQASSDSPCSLRKFYASLREPDPKKRKTIHQDVHINTINLFLCVAFLCVSKEADSDRESANESEDTSGYDSTASEPLSHMLPCLSLENVVLPSPECLHHAADIWSMCRWIYMLNSVFQKQFHRLGGFQVCHELIFMIIQKLFRSHTEDQGRRQGEMSRNENQELIRISYPELTLKGDVSSATAPDLGFLRKSADSVRGFQSQPVLPTSAEQIVATESVPGERKAFMSQQSETSLQSIRLLESLLAICLHSARACQQKMELELPSQGLSVENILCELREHLSQSKVAETELAKPLFDALLRVALGNHSADLGPGDAVTEKSHPSEEELLSQPGDFSEEAEDSQCCSLKLLGEEEGYEADSESNPEDVDTQDDGVELNPEAEGFSGSIVSNNLLENLTHGEIIYPEICMLGLNLLSASKAKLDVLAHVFESFLKIVRQKEKNISLLIQQGTVKILLGGFLNILTQTNSDFQACQRVLVDLLVSLMSSRTCSEDLTLLLRIFLEKSPCTEILLLGIHKIVESDFTMSPSQCLTFPFLHTPSLSNGVLSQKPPGILNSKALGLLRRARISRGKKEADRESFPYRLLSSWHIAPIHLPLLGQNCWPHLSEGFSVSLWFNVEYIHESESAAERGKRVKKRNKPSVLEDSSFEGAGDRPEVTESINPGDRLIEDGCIHLISLGSKALMIQVWADPHSGTFIFRVCMDSNDDTKAVSLAQVESQENIFFPSKWQHLVLTYIQHPQGKKNVHGEISIWVSGQRKTDVILDFVLPRKTSLSSDSNKTFCMIGHCLTSQEESLQLAGKWDLGNLLLFNGAKIGSQEAFFLYACGPNYTSIMPCKYGQPVIDYSKYINKDILRCDEIRDLFMTKKEVDVGLLIESLSVVYTTCCPAQYTIYEPVIRLKGQVKTQPSQRPFSSKEAQSILLEPSQLKGLQPTECKAIQGILHEIGGAGTFVFLFARVVELSSCEETQALALRVILSLIKYSQQRTQELENCNGLSMIHQVLVKQKCIVGFHILKTLLEGCCGEEVIHVSEHGEFKLDVESHAIIQDVKLLQELLLDWKIWNKAEQGVWETLLAALEVLIRVEHHQQQFNIKQLLNAHVVHHFLLTCQVLQEHREGQLTSMPREVCRSFVKIIAEVLGSPPDLELLTVIFNFLLAVHPPTNTYVCHNPTNFYFSLHIDGKIFQEKVQSLAYLRHSSSGGQAFPSPGFLVISPSAFTAAPPEGTSSSNIVPQRMAAQMVRSRSLPAFPTYLPLIRAQKLAASLGFSVDKLQNIADANPEKQNLLGRPYALKTSKEEAFISSCESAKTVCEMEALLGAHASANGVSRGSPRFPRARVDHKDVGTEPRSDDDSPGDESYPRRPDNLKGLASFQRSQSTVASLGLAFPSQNGSAVASRWPSLVDRNADDWENFTFSPAYEASYNRATSTHSVIEDCLIPICCGLYELLSGVLLVLPDAMLEDVMDRIIQADILLVLVNHPSPAIQQGVIKLLHAYINRASKEQKDKFLKNRGFSLLANQLYLHRGTQELLECFVEMFFGRPIGLDEEFDLEEVKHMELFQKWSVIPVLGLIETSLYDNVLLHNALLLLLQVLNSCSKVADMLLDNGLLYVLCNTVAALNGLEKNIPVNEYKLLACDIQQLFIAVTIHACSSSGTQYFRVIEDLIVLLGYLHNSKNKRTQNMALALQLRVLQAALEFIRSTANHDSESPVHSPSAHRHSVPPKRRSIAGSIPVKGSVTPYPPRRHFGSYGQLPMPFFSPLDQDSPQLSPGCTTCAASPRAGSRKFPLAQTESLLMKMRSVASDELHSMMQRRMSQEHPSQASEAELAQRLQRLTILAVNRIIYQELNSDIIDILRTPENTSQSKTSVSQTEISEEDMHHEQPSVYNPFQKEMLTYLLDGFKVCIGSSKTSVSKQQWTKILGSCKETLRDQLGRLLAHILSPTHTVQERKQILEIVHEPAHQDILRDCLSPSPQHGAKLVLYLSELIHNHQDELSEEEMDTAELLMNALKLCGHKCIPPSAPSKPELIKIIREEQKKYESEESVSKGSWQKTVNNNQQSLFQRLDFKSKDISKIAADITQAVSLSQGIERKKVIQHIRGMYKVDLSASRHWQECIQQLTHDRAVWYDPIYYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLRDRQKSEGVLRPPLSYLFEDKTHSSFSSTVKDKAASESIRVNRRCISVAPSRETAGELLLGKCGMYFVEDNASDAVESSSLQGELEPASFSWTYEEIKEVHRRWWQLRDNAVEIFLTNGRTLLLAFDNNKVRDDVYQSILTNNLPNLLEYGNITALTNLWYSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILSDYVSETLDLNDPSIYRNLSKPIAVQYKEKEDRYVDTYKYLEEEYRKGAREDDPMPPVQPYHYGSHYSNSGTVLHFLVRMPPFTKMFLAYQDQSFDIPDRTFHSTNTTWRLSSFESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNGERVNHVNLPPWARNDPRLFILIHRQALESDHVSQNICHWIDLVFGYKQKGKASVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFHTAHASRPGAKLNIEGELPAAVGLLVQFAFRETREPVKEVTHPSPLSWIKGLKWGEYVGSPSAPVPVVCFSQPHGERFGSLQALPTRAICGLSRNFCLLMTYNKEQGVRSMNNTNIQWSAILSWGYADNILRLKSKQSEPPINFIQSSQQHQVTSCAWVPDSCQLFTGSKCGVITAYTNRLTSSTPSEIEMESQMHLYGHTEEITGLCVCKPYSVMISVSRDGTCIVWDLNRLCYVQSLAGHKSPVTAVSASETSGDIATVCDSAGGGSDLRLWTVNGDLVGHVHCREIICSVAFSNQPEGVSINVIAGGLENGIVRLWSTWDLKPVREITFPKSNKPIISLTFSCDGHHLYTANSEGTVIAWCRKDQQRVKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_006516562.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysosomal trafficking regulator (Lyst), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516562.1

ORF Insert Sequence:

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TTGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AAGAGGAGAC ACACATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTGATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTTCTTCT GTCGCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CAGCAGGCAA CAGATTTTAA CCTGCCACTG 
TCATCTGATA TAATCCTGAC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCATCT GGTCAGGTTT CTGCAAAAGT AAACCTTTTT 
CGAAAAATCA GGCGACAGCG TAAAAGTACC CATCGTTATT CTGTAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAAGAGTAC GGTTGTGAGT 
AAACACAGGA GGCTCCACGC GCTGCCACGG TTCCTGACGC AGTCTCCTAA GGAAGGCCAC 
CTCGTAGCCA AACCTGACCC CTCTGCCACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
GTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGTGACAT ATTAAGTGAG 
CCAGCTGCTT TGTCTATTCT CAGTAACATG AATAATTCTC CTTTTGACTT ATGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA GGGACAACTG GTTTCTGCAG GTTGGACAGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGAACC GTGCTGCACC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATTTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGAGCCTT TCTCCCCGAG ACAAAAGAAA 
ACACTACAGG AGGTCCAGGA GGGCTTTGTA TTTTCCAAGT ATCGTCACCG AGCCCTTCTA 
CTACCTGAGC TTCTGGAAGG AGTTCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAA 
GGATTTAATC TCTTTGAAAC AGCAGTTCTT CAGATGGAAT GGCTGCTTTC AAGGGACGGT 
GTTCCTTCAG AAGCTGCAGA ACATTTGAAA GCTCTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGA 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GCGATCTTTC AGGGCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTAAA AGCCCCTTTG AAGAGACCGC AGAGGGAGAT 
GTGCAGTATC CAGAGCGCTG CTGCTGCATC GCCGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTGCAGCAGG TTTCCCTGAG CACCACGTGT GTCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAGTCT GTGATCGTCC CTTTACTGCA TGCTTTTAAG 
TTGCCAGCAC TGAAAGCTTT CCAGCAGCAT ATACTGAATG TCCTGAGCAA ACTTCTTTTG 
GATCAGTTAG GAGGAGCAGA GCTATCACCG AGAATTAAAA AAGCAGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGAGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTA CCTCCGGCTT GCCCAGTCCT TCCTACCGAT TTCAGGGGAT CCTGCCCAGC 
AGCGGCTCTG AAGACTTGCT GTGGAAGTGG GATGCATTAG AGGCTTATCA GAGCTTTGTC 
TTTCAAGAAG ACAGATTACA TAACATTCAG ATTGCAAATC ACATTTGTAA TTTAATCCAG 
AAAGGCAATG TAGTTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTCCTTCAGC TCAGACTCAC 
ATGTGTAGCC AACTGAAACA GTATTTGCCT CAGGAAGTGC TTCAGATTTA TTTAAAAACT 
CTACCTGTCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AACCAAATAA TTGAACTAAA TTACTTAGAT GGGATTCGAA GTCATTCCCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAAGATG CTGCAGTTCT AGACGTCGAT 
GGGTTAGACA TCCAACAGGA GTTGCCGTCC TTAAGTGTGG GTCCTTCTCT TCATAAGCAG 
CAAGCTTCTT CAGATTCTCC TTGCAGTCTC AGGAAGTTTT ATGCCAGCCT CAGAGAGCCT 
GATCCAAAAA AACGAAAGAC CATTCACCAG GATGTTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCAGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGC ACTGCCAGTG AGCCATTAAG TCACATGCTA 
CCATGTCTGT CTCTTGAGAA CGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCATGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGGCTTG GTGGTTTCCA AGTGTGCCAT GAATTAATAT TTATGATAAT CCAGAAACTA 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTAG AAATGAAAAC 
CAAGAGCTAA TCAGGATATC TTACCCCGAG CTGACACTGA AGGGAGATGT ATCATCTGCA 
ACAGCACCAG ACCTGGGATT TCTGAGAAAG AGTGCTGACA GCGTGCGTGG ATTCCAGTCA 
CAGCCTGTGC TTCCCACAAG TGCAGAGCAG ATTGTGGCTA CTGAATCTGT TCCTGGGGAA 
CGAAAGGCAT TTATGAGTCA ACAAAGTGAG ACTTCTCTCC AGAGCATACG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCATAGTGCC AGAGCCTGTC AACAGAAGAT GGAATTGGAG 
CTACCGTCTC AGGGCTTGTC TGTGGAAAAT ATATTGTGTG AACTGAGGGA ACACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGGC CCTGGTGATG CTGTGACTGA GAAGAGTCAT 
CCCTCTGAGG AAGAGCTGTT GTCCCAGCCC GGAGATTTTT CAGAAGAAGC TGAGGATTCT 
CAGTGTTGTA GTTTGAAACT TCTGGGTGAG GAAGAAGGCT ATGAAGCGGA TAGTGAAAGC 
AATCCTGAGG ATGTTGACAC CCAAGACGAT GGAGTAGAAT TAAATCCTGA AGCAGAAGGT 
TTCAGTGGAT CGATTGTTTC AAACAACTTA CTTGAAAACC TCACTCACGG GGAAATAATA 
TACCCTGAGA TTTGCATGCT GGGATTAAAT TTGCTTTCTG CTAGCAAAGC TAAACTTGAT 
GTGCTTGCTC ATGTGTTTGA GAGCTTTCTG AAAATTGTCA GGCAGAAGGA AAAGAACATT 
TCTCTCCTCA TACAACAGGG AACTGTGAAA ATCCTTCTAG GCGGGTTCTT GAATATTTTA 
ACACAAACTA ACTCTGATTT CCAAGCATGC CAGAGAGTAC TGGTGGATCT CTTGGTATCT 
TTGATGAGCT CAAGAACGTG TTCAGAAGAC TTAACTCTTC TTTTGAGAAT ATTTCTGGAG 
AAATCTCCTT GTACAGAAAT TCTTCTCCTT GGTATTCACA AAATTGTTGA AAGTGATTTT 
ACTATGAGCC CTTCACAGTG TCTGACCTTT CCTTTCCTGC ATACCCCGAG TTTAAGCAAT 
GGTGTCTTAT CACAGAAACC TCCTGGGATT CTTAACAGTA AAGCCTTAGG CTTATTGAGA 
AGAGCACGGA TTTCCCGAGG CAAGAAAGAG GCTGATAGAG AGAGTTTTCC CTATAGGCTG 
CTTTCCTCTT GGCATATAGC CCCAATCCAC CTGCCGTTGC TGGGACAGAA CTGCTGGCCA 
CACCTGTCAG AAGGATTTAG TGTTTCTCTG TGGTTTAATG TGGAATACAT CCATGAATCC 
GAGAGTGCTG CAGAAAGGGG AAAAAGAGTA AAGAAAAGAA ACAAACCATC AGTTCTGGAA 
GACAGCAGTT TTGAAGGAGC AGGTGATAGA CCAGAAGTTA CAGAATCCAT CAATCCTGGT 
GACAGACTCA TAGAAGACGG CTGTATTCAT TTGATTTCAC TGGGGTCCAA AGCATTGATG 
ATCCAAGTGT GGGCTGATCC CCACAGTGGC ACTTTTATCT TTCGTGTGTG CATGGACTCA 
AATGATGACA CGAAAGCTGT CTCACTAGCA CAGGTGGAAT CACAGGAGAA TATTTTCTTT 
CCAAGCAAAT GGCAACACTT AGTACTTACC TATATTCAGC ATCCTCAAGG GAAAAAGAAT 
GTCCATGGGG AAATCTCCAT ATGGGTCTCT GGGCAGAGGA AGACTGATGT CATCTTGGAT 
TTTGTGCTCC CAAGAAAAAC AAGCTTATCA TCAGACAGCA ATAAAACATT TTGCATGATT 
GGTCATTGCT TAACATCCCA AGAAGAGTCT CTGCAATTAG CTGGAAAATG GGACCTGGGG 
AACTTGCTCC TCTTCAATGG AGCTAAAATT GGCTCACAAG AGGCCTTTTT CCTGTATGCT 
TGTGGACCCA ACTACACATC CATCATGCCG TGTAAATATG GACAGCCAGT CATTGACTAC 
TCCAAATACA TTAATAAAGA CATTTTGAGA TGTGATGAAA TCAGAGACCT TTTTATGACC 
AAGAAAGAAG TGGATGTTGG TCTCTTAATT GAAAGTCTTT CAGTTGTTTA TACAACTTGC 
TGTCCTGCTC AGTACACCAT CTATGAACCA GTGATTCGAC TCAAGGGCCA AGTGAAAACT 
CAGCCCTCTC AAAGACCCTT CAGCTCAAAG GAAGCCCAGA GCATCTTGCT AGAACCTTCT 
CAACTCAAAG GCCTCCAACC TACGGAATGT AAAGCCATCC AGGGCATTCT GCATGAGATT 
GGTGGGGCTG GCACATTTGT TTTTCTCTTT GCTAGGGTTG TTGAACTTAG TAGCTGTGAA 
GAAACTCAAG CATTAGCACT GCGGGTTATA CTGTCTTTAA TTAAGTACAG CCAACAGAGA 
ACACAGGAAC TGGAAAATTG TAATGGACTC TCTATGATTC ACCAAGTGTT GGTCAAACAG 
AAATGCATTG TTGGCTTTCA CATTTTGAAG ACCCTTCTTG AAGGTTGCTG CGGTGAAGAA 
GTTATCCACG TCAGTGAGCA TGGAGAGTTC AAGCTGGATG TTGAGTCTCA TGCTATAATC 
CAAGATGTTA AGCTGCTGCA GGAACTGTTA CTTGACTGGA AGATATGGAA TAAGGCAGAG 
CAAGGTGTGT GGGAGACTCT GCTAGCAGCT TTGGAAGTCC TCATCCGGGT AGAGCACCAC 
CAGCAGCAGT TTAATATTAA GCAGTTGCTG AACGCCCACG TGGTTCACCA CTTCCTACTG 
ACCTGTCAGG TTTTACAGGA ACACAGAGAG GGGCAGCTTA CATCTATGCC CCGAGAAGTT 
TGTAGATCAT TTGTGAAAAT CATTGCAGAA GTCCTTGGTT CTCCTCCAGA CTTGGAATTA 
TTGACAGTTA TTTTCAATTT CCTGTTAGCT GTACACCCTC CTACTAATAC TTATGTTTGT 
CACAATCCCA CAAACTTCTA CTTCTCTTTG CACATAGATG GCAAGATCTT TCAGGAGAAA 
GTGCAGTCAC TCGCGTACCT GAGGCATTCT AGCAGCGGAG GGCAAGCCTT TCCCAGCCCT 
GGATTCCTGG TAATAAGCCC ATCTGCCTTT ACTGCAGCTC CTCCTGAAGG AACCAGTTCT 
TCCAATATTG TTCCACAGCG GATGGCTGCT CAGATGGTTC GATCTAGAAG TCTACCAGCA 
TTTCCTACTT ATTTACCACT AATACGAGCA CAAAAACTGG CTGCAAGTTT GGGTTTTAGT 
GTTGACAAGT TACAAAATAT TGCAGATGCC AACCCAGAGA AACAGAATCT TTTAGGAAGA 
CCCTACGCAC TGAAAACAAG CAAAGAGGAA GCATTCATCA GCAGCTGTGA GTCTGCAAAG 
ACTGTTTGTG AAATGGAGGC TCTTCTTGGA GCCCACGCCT CTGCCAATGG GGTTTCCAGA 
GGATCACCGA GGTTCCCCAG GGCCAGAGTA GATCACAAAG ATGTGGGAAC AGAGCCCAGA 
TCAGATGATG ACAGTCCTGG GGATGAGTCT TACCCACGTC GGCCTGACAA CCTCAAGGGA 
CTGGCCTCAT TCCAGCGAAG CCAAAGCACT GTCGCAAGCC TTGGGCTGGC GTTTCCCTCT 
CAGAATGGAT CTGCAGTTGC TAGCAGGTGG CCAAGTCTTG TTGATAGGAA TGCTGATGAC 
TGGGAGAACT TTACCTTTTC TCCTGCTTAT GAGGCAAGCT ACAACCGAGC CACAAGCACC 
CACAGTGTCA TTGAAGACTG TCTGATACCT ATCTGCTGTG GATTATATGA ACTCTTAAGT 
GGGGTTCTTC TTGTCCTGCC TGATGCTATG CTTGAAGATG TGATGGACAG GATTATTCAA 
GCAGATATTC TTCTAGTCCT TGTTAACCAC CCATCACCTG CTATCCAGCA AGGAGTAATT 
AAACTGTTAC ATGCATACAT TAATAGAGCA TCAAAGGAGC AAAAGGACAA GTTTCTGAAG 
AACCGTGGCT TTTCCTTATT AGCCAACCAG TTGTATCTTC ATAGGGGAAC TCAGGAGTTG 
TTGGAGTGCT TTGTTGAAAT GTTCTTTGGT CGACCGATTG GCCTGGATGA AGAATTTGAT 
CTGGAGGAAG TGAAGCACAT GGAACTGTTC CAGAAGTGGT CTGTCATTCC CGTTCTCGGA 
CTAATAGAGA CCTCTCTCTA TGACAATGTC CTCTTGCACA ATGCTCTTTT ACTTCTTCTG 
CAAGTTTTAA ACTCTTGTTC CAAGGTAGCA GACATGCTAC TGGACAATGG TCTACTCTAT 
GTATTATGTA ATACAGTAGC AGCCCTGAAT GGATTAGAAA AGAACATTCC TGTGAACGAA 
TACAAATTGC TCGCATGTGA TATACAGCAG CTTTTCATAG CAGTTACAAT TCATGCTTGC 
AGTTCCTCAG GCACACAGTA TTTTAGAGTG ATTGAAGACC TTATTGTACT TCTTGGATAT 
CTTCATAATA GCAAAAACAA GAGGACACAA AATATGGCTT TGGCCCTGCA GCTTAGAGTT 
CTCCAGGCTG CTTTGGAATT TATAAGGAGC ACAGCCAATC ATGACTCTGA AAGTCCAGTG 
CACTCGCCTT CTGCCCACCG CCATTCAGTG CCTCCGAAGC GGAGAAGCAT TGCTGGTTCA 
ATTCCAGTGA AGGGTTCTGT TACTCCTTAT CCACCAAGAC GTCACTTTGG TTCCTATGGT 
CAACTTCCGA TGCCCTTCTT CAGTCCATTA GATCAAGACT CCCCTCAGCT CTCTCCTGGA 
TGCACCACAT GTGCCGCATC CCCCAGAGCA GGTTCTCGCA AATTCCCTCT GGCTCAGACA 
GAGTCTCTGC TGATGAAGAT GCGCTCAGTG GCCAGCGATG AGCTACACTC TATGATGCAG 
AGGAGGATGA GCCAAGAGCA CCCCAGCCAG GCCTCGGAGG CAGAGCTCGC TCAGAGGCTG 
CAGAGGCTCA CCATCTTAGC TGTGAACAGG ATTATTTACC AAGAGTTGAA TTCAGATATT 
ATTGACATTT TGAGAACTCC AGAAAATACA TCCCAAAGCA AGACCTCAGT TTCTCAGACT 
GAAATTTCTG AAGAAGACAT GCATCATGAG CAACCTTCTG TATATAATCC ATTTCAAAAA 
GAAATGTTAA CCTATCTGTT GGATGGCTTC AAAGTGTGTA TTGGTTCAAG TAAAACTAGC 
GTTTCTAAGC AGCAGTGGAC TAAAATCCTG GGGTCTTGTA AAGAAACCCT CCGAGACCAG 
CTTGGAAGAT TGCTAGCGCA TATTTTGTCT CCAACCCACA CTGTACAAGA ACGGAAGCAG 
ATACTTGAGA TAGTTCATGA ACCAGCTCAC CAGGATATAC TTCGTGACTG TCTTAGCCCC 
TCCCCACAAC ATGGAGCCAA GTTGGTTTTG TATTTGTCAG AGTTGATACA TAATCATCAG 
GATGAGTTAA GTGAAGAAGA AATGGACACA GCAGAACTGC TTATGAATGC TCTAAAGTTA 
TGTGGCCACA AGTGCATCCC GCCCAGTGCC CCTTCCAAAC CAGAGCTCAT TAAGATCATC 
AGAGAGGAGC AAAAGAAGTA TGAAAGTGAA GAGAGTGTGA GCAAAGGCTC ATGGCAGAAA 
ACGGTGAACA ACAACCAGCA AAGTCTCTTC CAGAGGCTCG ATTTCAAATC CAAGGATATA 
TCTAAAATCG CTGCAGACAT CACCCAGGCT GTATCACTCT CCCAAGGCAT TGAAAGGAAG 
AAGGTGATCC AGCACATCAG AGGGATGTAC AAAGTTGACC TGAGTGCCAG CAGGCACTGG 
CAGGAATGCA TCCAGCAGCT GACACATGAC AGAGCAGTCT GGTATGACCC AATCTACTAT 
CCAACTTCAT GGCAGTTGGA TCCAACAGAA GGGCCAAACC GAGAGAGGAG ACGTTTGCAG 
AGATGCTATC TAACTATTCC CAATAAGTAC CTCCTGAGGG ACAGACAGAA GTCAGAAGGT 
GTGCTCAGGC CCCCACTCTC TTACCTTTTT GAAGATAAAA CTCATTCTTC CTTCTCCTCT 
ACTGTCAAAG ACAAAGCTGC AAGTGAATCC ATCAGAGTGA ATCGAAGATG TATCAGTGTT 
GCACCATCTA GAGAGACAGC TGGGGAATTG TTGTTAGGTA AATGTGGGAT GTACTTTGTG 
GAAGACAATG CCTCTGACGC AGTTGAAAGC TCGAGCCTCC AAGGGGAGTT AGAGCCGGCA 
TCATTTTCTT GGACATATGA GGAAATTAAA GAAGTTCACA GGCGCTGGTG GCAACTAAGA 
GATAATGCTG TAGAAATCTT TTTAACAAAT GGCAGAACAC TCCTATTAGC ATTTGACAAT 
AACAAGGTTC GTGATGACGT GTACCAGAGC ATCCTCACAA ATAACCTCCC AAATCTTCTG 
GAGTACGGCA ACATCACCGC TCTGACAAAC CTGTGGTATT CTGGACAAAT TACCAATTTT 
GAATATTTGA CTCATTTAAA CAAGCATGCG GGCCGGTCCT TCAATGATCT CATGCAGTAC 
CCGGTGTTCC CCTTCATCCT TTCTGACTAT GTTAGTGAGA CTCTTGACCT CAATGATCCA 
TCTATCTACA GAAACCTATC TAAGCCTATA GCTGTGCAGT ATAAAGAAAA AGAAGACCGT 
TACGTTGACA CATACAAGTA CTTGGAGGAG GAGTATCGCA AGGGAGCTCG AGAGGATGAC 
CCCATGCCTC CTGTGCAACC CTACCACTAT GGCTCCCACT ACTCCAACAG CGGCACCGTG 
CTCCACTTCC TGGTCAGGAT GCCGCCTTTC ACTAAAATGT TTCTAGCCTA TCAAGATCAG 
AGTTTCGACA TTCCAGACCG AACATTTCAT TCTACAAACA CAACTTGGCG CCTCTCCTCC 
TTTGAGTCCA TGACTGATGT GAAGGAGCTG ATTCCAGAGT TTTTCTATCT TCCTGAGTTC 
TTAGTGAACC GTGAAGGCTT TGACTTCGGT GTTCGTCAGA ATGGAGAGCG GGTTAACCAC 
GTCAATCTTC CTCCCTGGGC ACGCAACGAT CCTCGGCTGT TCATCCTTAT TCACCGGCAA 
GCACTAGAGT CTGACCATGT GTCCCAGAAC ATCTGTCACT GGATCGACTT AGTGTTTGGC 
TACAAGCAAA AGGGGAAGGC GTCTGTTCAA GCCATCAATG TCTTCCACCC TGCTACATAT 
TTTGGAATGG ATGTCTCTGC AGTTGAAGAT CCAGTGCAGA GACGGGCTTT AGAAACCATG 
ATAAAAACCT ACGGGCAGAC CCCACGTCAG TTGTTCCACA CAGCCCATGC CAGCCGACCT 
GGAGCCAAGC TTAACATCGA AGGAGAGCTT CCAGCAGCTG TTGGCTTGTT AGTCCAGTTC 
GCTTTCAGAG AGACCCGAGA ACCAGTCAAG GAAGTCACTC ATCCGAGCCC TTTGTCATGG 
ATAAAAGGCT TGAAGTGGGG GGAGTACGTA GGTTCCCCCA GTGCTCCAGT ACCTGTGGTC 
TGCTTCAGCC AGCCCCATGG AGAAAGATTT GGTTCCCTGC AGGCACTGCC CACCAGAGCC 
ATCTGTGGTT TATCACGAAA CTTCTGTCTT CTGATGACCT ACAACAAGGA GCAAGGTGTG 
AGAAGCATGA ACAACACCAA TATTCAGTGG TCTGCTATCC TAAGCTGGGG ATATGCTGAC 
AACATCTTAC GGTTGAAAAG TAAGCAGAGT GAGCCACCAA TCAACTTCAT TCAGAGTTCA 
CAGCAGCACC AGGTAACCAG TTGTGCCTGG GTGCCTGACA GTTGTCAGCT CTTCACTGGG 
AGCAAGTGTG GTGTCATCAC AGCCTATACC AACAGGCTCA CCAGCAGCAC GCCCTCAGAA 
ATTGAAATGG AGAGTCAGAT GCATCTCTAT GGACACACAG AGGAGATCAC CGGCTTATGT 
GTCTGCAAGC CGTACAGCGT GATGATAAGC GTGAGCAGAG ACGGGACCTG CATAGTATGG 
GACCTGAACA GGCTGTGCTA TGTACAAAGT TTGGCTGGAC ACAAAAGCCC TGTGACGGCT 
GTCTCTGCCA GTGAAACGTC AGGTGACATT GCTACTGTGT GTGACTCAGC TGGCGGGGGC 
AGTGACCTGA GACTCTGGAC CGTGAATGGG GACCTCGTTG GACATGTCCA CTGCAGAGAG 
ATCATTTGTT CTGTAGCTTT CTCCAACCAG CCTGAGGGAG TCTCCATCAA CGTCATTGCT 
GGGGGATTAG AAAATGGCAT TGTAAGGCTA TGGAGCACAT GGGACTTGAA GCCTGTGAGA 
GAGATTACAT TTCCCAAATC AAATAAGCCC ATCATAAGCC TGACATTCTC CTGTGATGGC 
CACCATTTGT ACACTGCCAA CAGTGAGGGG ACAGTGATCG CATGGTGCCG GAAGGACCAG 
CAGCGTGTGA AGCTGCCCAT GTTCTACTCT TTCCTCAGCA GCTACGCAGC TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reversible immortalisation enables genetic correction of human muscle progenitors and engineering of next-generation human artificial chromosomes for Duchenne muscular dystrophy.
EMBO molecular medicine(2017 Dec)
Benedetti S,Uno N,Hoshiya H,Ragazzi M,Ferrari G,Kazuki Y,Moyle LA,Tonlorenzi R,Lombardo A,Chaouch S,Mouly V,Moore M,Popplewell L,Kazuki K,Katoh M,Naldini L,Dickson G,Messina G,Oshimura M,Cossu G,Tedesco FS

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Lyst cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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