Prima1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prima1
Entrez Gene ID	170952
Full Name	proline rich membrane anchor 1
Synonyms	B230212M13Rik,BB120497,PRiMA
General protein information	Preferred Names proline rich membrane anchor 1 Names proline-rich membrane anchor 1 acetylcholinesterase membrane anchor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 E

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515506.2	XP_006515569.1	proline-rich membrane anchor 1 isoform X1
NM_133364.2	NP_579942.2	proline-rich membrane anchor 1 precursor
XM_006515507.4	XP_006515570.1	proline-rich membrane anchor 1 isoform X1
XM_006515507.3	XP_006515570.1	proline-rich membrane anchor 1 isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	prima1	XP_002933274.1
Bos taurus (cattle)	PRIMA1	NP_001070955.1
Pan troglodytes (chimpanzee)	PRIMA1	XP_003952611.1
Mus musculus (house mouse)	Prima1	NP_579942.2
Gallus gallus (chicken)	PRIMA1	NP_001138964.1
Homo sapiens (human)	PRIMA1	NP_821092.1
Canis lupus familiaris (dog)	PRIMA1	XP_854468.1
Rattus norvegicus (Norway rat)	Prima1	NP_001102191.2

Gene Ontology
Bibliography

Related articles in PubMed

Dysfunctional Presynaptic M2 Receptors in the Presence of Chronically High Acetylcholine Levels: Data from the PRiMA Knockout Mouse.
Mohr F, Krejci E, Zimmermann M, Klein J
PloS one10(10)e0141136(2015)

Schwann cells sense and control acetylcholine spillover at the neuromuscular junction by ?7 nicotinic receptors and butyrylcholinesterase.
Petrov KA, Girard E, Nikitashina AD, Colasante C, Bernard V, Nurullin L, Leroy J, Samigullin D, Colak O, Nikolsky E, Plaud B, Krejci E
The Journal of neuroscience : the official journal of the Society for Neuroscience34(36)11870-83(2014 Sep)

Presenilin-1 influences processing of the acetylcholinesterase membrane anchor PRiMA.
Garc?a-Ayll?n MS, Campanari ML, Montenegro MF, Cuchillo-Ib??ez I, Belbin O, Lle? A, Tsim K, Vidal CJ, S?ez-Valero J
Neurobiology of aging35(7)1526-36(2014 Jul)

Subunit composition and kinetics of the Renshaw cell heteromeric nicotinic receptors.
Lamotte d'Incamps B, Ascher P
Biochemical pharmacology86(8)1114-21(2013 Oct)

Developmental adaptation of central nervous system to extremely high acetylcholine levels.
Farar V, Hrabovska A, Krejci E, Myslivecek J
PloS one8(7)e68265(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It interacts with PS1 and undergoes proteolytic processing.
Title: Presenilin-1 influences processing of the acetylcholinesterase membrane anchor PRiMA.

the relative content of AChE, BuChE and PRiMA mRNAs were compared in normal and Lama2dy muscle and sciatic nerve
Title: The AChE membrane-binding tail PRiMA is down-regulated in muscle and nerve of mice with muscular dystrophy by merosin deficiency.

Elevated expression of muscarinic receptors is responsible for physiological adaptation of PRiMA-deficient developing mice.
Title: Developmental adaptation of central nervous system to extremely high acetylcholine levels.

The N-linked glycosylation of AChE(T) plays a major role for acquisition of AChE full enzymatic activity but does not affect its oligomerization.
Title: The assembly of proline-rich membrane anchor (PRiMA)-linked acetylcholinesterase enzyme: glycosylation is required for enzymatic activity but not for oligomerization.

Data show that along the nerve terminus the vast majority of acetylcholinesterase is anchored by collagen Q that is only produced by the muscle, whereas very minor amounts of AChE are anchored by PRiMA that is produced by motoneurons.
Title: Distinct localization of collagen Q and PRiMA forms of acetylcholinesterase at the neuromuscular junction.

The following Prima1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prima1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10023	NM_133364.2 Latest version!	Mus musculus proline rich membrane anchor 1 (Prima1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu10023	XM_006515506.2 Latest version!	Mus musculus proline rich membrane anchor 1 (Prima1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu10023	XM_006515507.4 Latest version!	Mus musculus proline rich membrane anchor 1 (Prima1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu10023	XM_006515507.3	Mus musculus proline rich membrane anchor 1 (Prima1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prima1 ( NM_133364.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_579942.2 Mus musculus proline rich membrane anchor 1 (Prima1), mRNA.

Price & Availability↑

CloneID	OMu10023
Clone ID Related Accession (Same CDS sequence)	XM_006515506.2 , NM_133364.2 , XM_006515507.4 , XM_006515507.3
Accession Version	NM_133364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	proline-rich membrane anchor 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK140034.1, AY043275.1 and AK039705.1. On or before May 4, 2006 this sequence version replaced XM_001052098.1, XM_001052097.1, XM_001052099.1, NM_133364.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY043275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGCTACTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAC 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACAC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCCCCTCTT 
CCCCCTCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CCGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CTGCAGAGGA CAGCTGGTGG TCTGGTCTGG TGATCATTGT CGCTGTGGTC 
TGTGCCTCCC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAGGATGA GAATGGCACC AGTGTCGCTG AGTACCCCAT GAGCTCCTCA 
CAGAGCCACA AAGGGGTGGA TGTGAACGCT GCAGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_579942.2
CDS	103..564
Translation	MLLRDLVLRHGCCWPSLLLHCALHPLWGLVQVTHAEPQKSCSKVTDSCQHICQCRPPPPLPPPPPPPPPPRLLSAPAPNSTSCPAEDSWWSGLVIIVAVVCASLVFLTVLVIICYKAIKRKPLRKDENGTSVAEYPMSSSQSHKGVDVNAAVV

Target ORF information:

RefSeq Version	NM_133364.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline rich membrane anchor 1 (Prima1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133364.2

ORF Insert Sequence:

ATGCTACTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAC 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACAC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCCCCTCTT 
CCCCCTCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CCGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CTGCAGAGGA CAGCTGGTGG TCTGGTCTGG TGATCATTGT CGCTGTGGTC 
TGTGCCTCCC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAGGATGA GAATGGCACC AGTGTCGCTG AGTACCCCAT GAGCTCCTCA 
CAGAGCCACA AAGGGGTGGA TGTGAACGCT GCAGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prima1 ( XM_006515506.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515569.1 Mus musculus proline rich membrane anchor 1 (Prima1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10023
Clone ID Related Accession (Same CDS sequence)	XM_006515506.2 , NM_133364.2 , XM_006515507.4 , XM_006515507.3
Accession Version	XM_006515506.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	proline-rich membrane anchor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006515506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAC 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACAC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCCCCTCTT 
CCCCCTCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CCGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CTGCAGAGGA CAGCTGGTGG TCTGGTCTGG TGATCATTGT CGCTGTGGTC 
TGTGCCTCCC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAGGATGA GAATGGCACC AGTGTCGCTG AGTACCCCAT GAGCTCCTCA 
CAGAGCCACA AAGGGGTGGA TGTGAACGCT GCAGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515569.1
CDS	127..588
Translation	MLLRDLVLRHGCCWPSLLLHCALHPLWGLVQVTHAEPQKSCSKVTDSCQHICQCRPPPPLPPPPPPPPPPRLLSAPAPNSTSCPAEDSWWSGLVIIVAVVCASLVFLTVLVIICYKAIKRKPLRKDENGTSVAEYPMSSSQSHKGVDVNAAVV

Target ORF information:

RefSeq Version	XM_006515506.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline rich membrane anchor 1 (Prima1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515506.2

ORF Insert Sequence:

ATGCTACTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAC 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACAC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCCCCTCTT 
CCCCCTCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CCGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CTGCAGAGGA CAGCTGGTGG TCTGGTCTGG TGATCATTGT CGCTGTGGTC 
TGTGCCTCCC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAGGATGA GAATGGCACC AGTGTCGCTG AGTACCCCAT GAGCTCCTCA 
CAGAGCCACA AAGGGGTGGA TGTGAACGCT GCAGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prima1 ( XM_006515507.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515570.1 Mus musculus proline rich membrane anchor 1 (Prima1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu10023
Clone ID Related Accession (Same CDS sequence)	XM_006515506.2 , NM_133364.2 , XM_006515507.4 , XM_006515507.3
Accession Version	XM_006515507.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	proline-rich membrane anchor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515507.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAC 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACAC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCCCCTCTT 
CCCCCTCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CCGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CTGCAGAGGA CAGCTGGTGG TCTGGTCTGG TGATCATTGT CGCTGTGGTC 
TGTGCCTCCC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAGGATGA GAATGGCACC AGTGTCGCTG AGTACCCCAT GAGCTCCTCA 
CAGAGCCACA AAGGGGTGGA TGTGAACGCT GCAGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515570.1
CDS	524..985
Translation	MLLRDLVLRHGCCWPSLLLHCALHPLWGLVQVTHAEPQKSCSKVTDSCQHICQCRPPPPLPPPPPPPPPPRLLSAPAPNSTSCPAEDSWWSGLVIIVAVVCASLVFLTVLVIICYKAIKRKPLRKDENGTSVAEYPMSSSQSHKGVDVNAAVV

Target ORF information:

RefSeq Version	XM_006515507.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline rich membrane anchor 1 (Prima1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515507.3

ORF Insert Sequence:

ATGCTACTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAC 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACAC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCCCCTCTT 
CCCCCTCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CCGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CTGCAGAGGA CAGCTGGTGG TCTGGTCTGG TGATCATTGT CGCTGTGGTC 
TGTGCCTCCC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAGGATGA GAATGGCACC AGTGTCGCTG AGTACCCCAT GAGCTCCTCA 
CAGAGCCACA AAGGGGTGGA TGTGAACGCT GCAGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prima1 ( XM_006515507.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515570.1 Mus musculus proline rich membrane anchor 1 (Prima1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu10023
Clone ID Related Accession (Same CDS sequence)	XM_006515506.2 , NM_133364.2 , XM_006515507.4 , XM_006515507.3
Accession Version	XM_006515507.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	proline-rich membrane anchor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515507.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAC 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACAC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCCCCTCTT 
CCCCCTCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CCGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CTGCAGAGGA CAGCTGGTGG TCTGGTCTGG TGATCATTGT CGCTGTGGTC 
TGTGCCTCCC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAGGATGA GAATGGCACC AGTGTCGCTG AGTACCCCAT GAGCTCCTCA 
CAGAGCCACA AAGGGGTGGA TGTGAACGCT GCAGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515570.1
CDS	522..983
Translation	MLLRDLVLRHGCCWPSLLLHCALHPLWGLVQVTHAEPQKSCSKVTDSCQHICQCRPPPPLPPPPPPPPPPRLLSAPAPNSTSCPAEDSWWSGLVIIVAVVCASLVFLTVLVIICYKAIKRKPLRKDENGTSVAEYPMSSSQSHKGVDVNAAVV

Target ORF information:

RefSeq Version	XM_006515507.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus proline rich membrane anchor 1 (Prima1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515507.4

ORF Insert Sequence:

ATGCTACTCC GGGACCTGGT GCTGCGCCAT GGCTGCTGCT GGCCCTCACT TCTGCTGCAC 
TGTGCGCTGC ACCCACTCTG GGGTTTAGTT CAGGTGACAC ACGCTGAGCC CCAGAAGTCT 
TGCTCTAAGG TGACTGACAG CTGCCAACAC ATCTGCCAGT GCCGGCCCCC ACCCCCTCTT 
CCCCCTCCAC CCCCACCTCC GCCACCTCCC AGACTACTCT CCGCCCCAGC TCCCAACTCT 
ACCTCCTGCC CTGCAGAGGA CAGCTGGTGG TCTGGTCTGG TGATCATTGT CGCTGTGGTC 
TGTGCCTCCC TGGTGTTTCT GACTGTCCTG GTCATCATTT GCTACAAAGC CATAAAGAGG 
AAACCACTGA GAAAGGATGA GAATGGCACC AGTGTCGCTG AGTACCCCAT GAGCTCCTCA 
CAGAGCCACA AAGGGGTGGA TGTGAACGCT GCAGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dysfunctional Presynaptic M2 Receptors in the Presence of Chronically High Acetylcholine Levels: Data from the PRiMA Knockout Mouse.
PloS one10(10)e0141136(2015)
Mohr F,Krejci E,Zimmermann M,Klein J

Schwann cells sense and control acetylcholine spillover at the neuromuscular junction by ?7 nicotinic receptors and butyrylcholinesterase.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(36)11870-83(2014 Sep)
Petrov KA,Girard E,Nikitashina AD,Colasante C,Bernard V,Nurullin L,Leroy J,Samigullin D,Colak O,Nikolsky E,Plaud B,Krejci E

Presenilin-1 influences processing of the acetylcholinesterase membrane anchor PRiMA.
Neurobiology of aging35(7)1526-36(2014 Jul)
Garc?a-Ayll?n MS,Campanari ML,Montenegro MF,Cuchillo-Ib??ez I,Belbin O,Lle? A,Tsim K,Vidal CJ,S?ez-Valero J

Subunit composition and kinetics of the Renshaw cell heteromeric nicotinic receptors.
Biochemical pharmacology86(8)1114-21(2013 Oct)
Lamotte d'Incamps B,Ascher P

Developmental adaptation of central nervous system to extremely high acetylcholine levels.
PloS one8(7)e68265(2013)
Farar V,Hrabovska A,Krejci E,Myslivecek J

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Prima1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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