Blnk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Blnk
Entrez Gene ID	17060
Full Name	B cell linker
Synonyms	BASH,Bca,Ly-57,Ly57,Lyw-57,SLP-65
General protein information	Preferred Names B cell linker Names B-cell linker protein B-cell adapter containing a SH2 domain protein B-cell adapter containing a Src homology 2 domain protein cytoplasmic adapter protein lymphocyte antigen 57 src homology 2 domain-containing leukocyte protein of 65 kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 C3\|19 34.26 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001365054.1	NP_001351983.1	B-cell linker protein isoform 2
NM_008528.5	NP_032554.2	B-cell linker protein isoform 1
NM_008528.4	NP_032554.2	B-cell linker protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BLNK	XP_001159266.1
	BLNK	XP_001100700.2
Canis lupus familiaris (dog)	BLNK	XP_005637631.1
Gallus gallus (chicken)	BLNK	NP_990239.1
Bos taurus (cattle)	BLNK	NP_001039519.1
Rattus norvegicus (Norway rat)	Blnk	NP_001020938.1
Xenopus tropicalis (tropical clawed frog)	blnk	NP_001072164.1
Mus musculus (house mouse)	Blnk	NP_032554.2
Danio rerio (zebrafish)	blnk	NP_998003.1
Homo sapiens (human)	BLNK	NP_037446.1

Gene Ontology
Bibliography

Related articles in PubMed

The BTG2-PRMT1 module limits pre-B cell expansion by regulating the CDK4-Cyclin-D3 complex.
Dolezal E, Infantino S, Drepper F, B?rsig T, Singh A, Wossning T, Fiala GJ, Minguet S, Warscheid B, Tarlinton DM, Jumaa H, Medgyesi D, Reth M
Nature immunology18(8)911-920(2017 Aug)

Development of SH2 probes and pull-down assays to detect pathogen-induced, site-specific tyrosine phosphorylation of the TLR adaptor SCIMP.
Luo L, Tong SJ, Wall AA, Khromykh T, Sweet MJ, Stow JL
Immunology and cell biology95(6)564-570(2017 Jul)

Regulation of B cell fate by chronic activity of the IgE B cell receptor.
Yang Z, Robinson MJ, Chen X, Smith GA, Taunton J, Liu W, Allen CD
eLife5(2016 12)

Autonomous membrane IgE signaling prevents IgE-memory formation.
Haniuda K, Fukao S, Kodama T, Hasegawa H, Kitamura D
Nature immunology17(9)1109-17(2016 09)

B Cell Linker Protein (BLNK) Is a Selective Target of Repression by PAX5-PML Protein in the Differentiation Block That Leads to the Development of Acute Lymphoblastic Leukemia.
Imoto N, Hayakawa F, Kurahashi S, Morishita T, Kojima Y, Yasuda T, Sugimoto K, Tsuzuki S, Naoe T, Kiyoi H
The Journal of biological chemistry291(9)4723-31(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The pre-B-cell receptor (pre-BCR) signaling molecules BLNK, BTK and BANK1 were positively regulated by the ZFP521 gene, leading to enhancement of the pre-BCR signaling pathway.
Title: ZFP521 contributes to pre-B-cell lymphomagenesis through modulation of the pre-B-cell receptor signaling pathway.

Authors demonstrate leukemogenicity of PAX5-PML by introducing it into normal mouse pro-B cells; B-cell linker protein (Blnk) is repressed by PAX5-PML in leukemia cells; enforced expression of Blnk increases survival despite introduction of PAX5-PML.
Title: B Cell Linker Protein (BLNK) Is a Selective Target of Repression by PAX5-PML Protein in the Differentiation Block That Leads to the Development of Acute Lymphoblastic Leukemia.

B-cell linker protein expression contributes to controlling allergic and autoimmune diseases by mediating IL-10 production in regulatory B cells.
Title: B-cell linker protein expression contributes to controlling allergic and autoimmune diseases by mediating IL-10 production in regulatory B cells.

Tumor suppressor BLNK is a target of transcriptional activation by PU.1 and Spi-B in the B cell lineage.
Title: Regulation of B cell linker protein transcription by PU.1 and Spi-B in murine B cell acute lymphoblastic leukemia.

CMTM7 functions to link sIgM and BLNK in the plasma membrane.
Title: Identification of CMTM7 as a transmembrane linker of BLNK and the B-cell receptor.

The following Blnk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Blnk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02152	NM_008528.5 Latest version!	Mus musculus B cell linker (Blnk), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu84383	NM_001365054.1 Latest version!	Mus musculus B cell linker (Blnk), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu02152	NM_008528.4	Mus musculus B cell linker (Blnk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Blnk ( NM_008528.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032554.2 Mus musculus B cell linker (Blnk), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02152
Clone ID Related Accession (Same CDS sequence)	NM_008528.5 , NM_008528.4
Accession Version	NM_008528.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	B-cell linker protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126608.20 and AC117253.3. On Jul 24, 2018 this sequence version replaced NM_008528.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y17159.1, SRR5189670.106963.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACAAGC TGAATAAGAT AACTGTCCCT GCCAGCCAGA AGCTGAGACA GCTTCAAAAG 
ATGGTCCATG ATATTAAGAA CAATGAAGGT GGAATAATGG ACAAGATAAA AAAGCTAAAA 
GTCAAAGGCC CTCCAAGTGT TCCTCGAAGG GACTACGCAT TAGACAGCCC TGCAGATGAA 
GAGGAGCAGT GGTCAGATGA CTTCGACAGT GACTATGAAA ATCCAGATGA ACATTCGGAC 
TCCGAGATGT ATGTGATGCC TGCCGAGGAG ACGGGCGACG ATTCCTATGA ACCGCCTCCC 
GCTGAGCAGC AGACACGGGT GGTCCATCCA GCCCTGCCCT TCACGAGGGG CGAGTATGTA 
GATAATCGAT CCAGCCAGCG GCACTCTCCG CCCTTCAGCA AGACACTTCC CAGTAAGCCC 
AGCTGGCCTT CAGCGAAAGC GAGGCTGGCC TCCACTCTGC CAGCCCCCAA CTCTCTACAG 
AAGCCTCAAG TCCCCCCCAA GCCCAAAGAC CTCCTTGAGG ATGAGGCTGA TTATGTGGTC 
CCTGTGGAAG ATAACGATGA AAACTATATC CATCCCAGAG AAAGCAGCCC GCCGCCTGCT 
GAGAAGGCTC CCATGGTGAA TAGATCAACC AAGCCAAACA GTTCCTCAAA GCACATGTCG 
CCTCCAGGGA CTGTCGCAGG TCGAAACAGT GGGGTCTGGG ACTCCAAGTC ATCTTTGCCT 
GCCGCACCAT CCCCACTACC ACGGGCTGGG AAGAAGCCAG CTACACCACT TAAGACTACT 
CCCGTTCCTC CCCTACCGAA TGCATCAAAT GTTTGTGAAG AAAAGCCTGT TCCTGCTGAG 
CGCCACCGAG GGTCTAGTCA CAGACAAGAC ACTGTACAGT CACCAGTGTT TCCTCCCACC 
CAGAAACCTG TCCATCAAAA GCCTGTACCC TTGCCAAGGT TCCCAGAAGC GGGGAGCCCA 
GCTGCAGATG GACCGTTCCA CAGCTTCCCA TTTAATTCGA CGTTTGCAGA CCAGGAGGCT 
GAACTGCTCG GTAAGCCCTG GTATGCTGGC GCCTGTGACC GCAAGTCTGC TGAAGAGGCC 
TTGCACAGAT CCAACAAGGA TGGATCGTTT CTTATTCGGA AGAGCTCTGG CCATGATTCC 
AAGCAGCCGT ACACCCTAGT TGCGTTCTTT AACAAGCGAG TGTATAATAT TCCTGTACGG 
TTTATTGAAG CAACCAAACA GTATGCTTTG GGAAAGAAGA AAAATGGTGA AGAGTACTTC 
GGAAGTGTTG TGGAAATCGT CAACAGTCAC CAGCACAACC CCCTGGTTCT TATTGACAGT 
CAGAATAACA CGAAAGATTC CACGAGACTG AAATATGCTG TGAAGGTTTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032554.2
CDS	164..1537
Translation	MDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMDKIKKLKVKGPPSVPRRDYALDSPADEEEQWSDDFDSDYENPDEHSDSEMYVMPAEETGDDSYEPPPAEQQTRVVHPALPFTRGEYVDNRSSQRHSPPFSKTLPSKPSWPSAKARLASTLPAPNSLQKPQVPPKPKDLLEDEADYVVPVEDNDENYIHPRESSPPPAEKAPMVNRSTKPNSSSKHMSPPGTVAGRNSGVWDSKSSLPAAPSPLPRAGKKPATPLKTTPVPPLPNASNVCEEKPVPAERHRGSSHRQDTVQSPVFPPTQKPVHQKPVPLPRFPEAGSPAADGPFHSFPFNSTFADQEAELLGKPWYAGACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLVAFFNKRVYNIPVRFIEATKQYALGKKKNGEEYFGSVVEIVNSHQHNPLVLIDSQNNTKDSTRLKYAVKVS

Target ORF information:

RefSeq Version	NM_008528.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus B cell linker (Blnk), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008528.5

ORF Insert Sequence:

ATGGACAAGC TGAATAAGAT AACTGTCCCT GCCAGCCAGA AGCTGAGACA GCTTCAAAAG 
ATGGTCCATG ATATTAAGAA CAATGAAGGT GGAATAATGG ACAAGATAAA AAAGCTAAAA 
GTCAAAGGCC CTCCAAGTGT TCCTCGAAGG GACTACGCAT TAGACAGCCC TGCAGATGAA 
GAGGAGCAGT GGTCAGATGA CTTCGACAGT GACTATGAAA ATCCAGATGA ACATTCGGAC 
TCCGAGATGT ATGTGATGCC TGCCGAGGAG ACGGGCGACG ATTCCTATGA ACCGCCTCCC 
GCTGAGCAGC AGACACGGGT GGTCCATCCA GCCCTGCCCT TCACGAGGGG CGAGTATGTA 
GATAATCGAT CCAGCCAGCG GCACTCTCCG CCCTTCAGCA AGACACTTCC CAGTAAGCCC 
AGCTGGCCTT CAGCGAAAGC GAGGCTGGCC TCCACTCTGC CAGCCCCCAA CTCTCTACAG 
AAGCCTCAAG TCCCCCCCAA GCCCAAAGAC CTCCTTGAGG ATGAGGCTGA TTATGTGGTC 
CCTGTGGAAG ATAACGATGA AAACTATATC CATCCCAGAG AAAGCAGCCC GCCGCCTGCT 
GAGAAGGCTC CCATGGTGAA TAGATCAACC AAGCCAAACA GTTCCTCAAA GCACATGTCG 
CCTCCAGGGA CTGTCGCAGG TCGAAACAGT GGGGTCTGGG ACTCCAAGTC ATCTTTGCCT 
GCCGCACCAT CCCCACTACC ACGGGCTGGG AAGAAGCCAG CTACACCACT TAAGACTACT 
CCCGTTCCTC CCCTACCGAA TGCATCAAAT GTTTGTGAAG AAAAGCCTGT TCCTGCTGAG 
CGCCACCGAG GGTCTAGTCA CAGACAAGAC ACTGTACAGT CACCAGTGTT TCCTCCCACC 
CAGAAACCTG TCCATCAAAA GCCTGTACCC TTGCCAAGGT TCCCAGAAGC GGGGAGCCCA 
GCTGCAGATG GACCGTTCCA CAGCTTCCCA TTTAATTCGA CGTTTGCAGA CCAGGAGGCT 
GAACTGCTCG GTAAGCCCTG GTATGCTGGC GCCTGTGACC GCAAGTCTGC TGAAGAGGCC 
TTGCACAGAT CCAACAAGGA TGGATCGTTT CTTATTCGGA AGAGCTCTGG CCATGATTCC 
AAGCAGCCGT ACACCCTAGT TGCGTTCTTT AACAAGCGAG TGTATAATAT TCCTGTACGG 
TTTATTGAAG CAACCAAACA GTATGCTTTG GGAAAGAAGA AAAATGGTGA AGAGTACTTC 
GGAAGTGTTG TGGAAATCGT CAACAGTCAC CAGCACAACC CCCTGGTTCT TATTGACAGT 
CAGAATAACA CGAAAGATTC CACGAGACTG AAATATGCTG TGAAGGTTTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blnk ( NM_008528.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032554.2 Mus musculus B cell linker (Blnk), mRNA.

Price & Availability↑

CloneID	OMu02152
Clone ID Related Accession (Same CDS sequence)	NM_008528.5 , NM_008528.4
Accession Version	NM_008528.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-28
Organism	Mus musculus(house mouse)
Product	B-cell linker protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC126608.20 and AC117253.3. On Nov 17, 2006 this sequence version replaced NM_008528.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y17159.1, AK089704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGACAAGC TGAATAAGAT AACTGTCCCT GCCAGCCAGA AGCTGAGACA GCTTCAAAAG 
ATGGTCCATG ATATTAAGAA CAATGAAGGT GGAATAATGG ACAAGATAAA AAAGCTAAAA 
GTCAAAGGCC CTCCAAGTGT TCCTCGAAGG GACTACGCAT TAGACAGCCC TGCAGATGAA 
GAGGAGCAGT GGTCAGATGA CTTCGACAGT GACTATGAAA ATCCAGATGA ACATTCGGAC 
TCCGAGATGT ATGTGATGCC TGCCGAGGAG ACGGGCGACG ATTCCTATGA ACCGCCTCCC 
GCTGAGCAGC AGACACGGGT GGTCCATCCA GCCCTGCCCT TCACGAGGGG CGAGTATGTA 
GATAATCGAT CCAGCCAGCG GCACTCTCCG CCCTTCAGCA AGACACTTCC CAGTAAGCCC 
AGCTGGCCTT CAGCGAAAGC GAGGCTGGCC TCCACTCTGC CAGCCCCCAA CTCTCTACAG 
AAGCCTCAAG TCCCCCCCAA GCCCAAAGAC CTCCTTGAGG ATGAGGCTGA TTATGTGGTC 
CCTGTGGAAG ATAACGATGA AAACTATATC CATCCCAGAG AAAGCAGCCC GCCGCCTGCT 
GAGAAGGCTC CCATGGTGAA TAGATCAACC AAGCCAAACA GTTCCTCAAA GCACATGTCG 
CCTCCAGGGA CTGTCGCAGG TCGAAACAGT GGGGTCTGGG ACTCCAAGTC ATCTTTGCCT 
GCCGCACCAT CCCCACTACC ACGGGCTGGG AAGAAGCCAG CTACACCACT TAAGACTACT 
CCCGTTCCTC CCCTACCGAA TGCATCAAAT GTTTGTGAAG AAAAGCCTGT TCCTGCTGAG 
CGCCACCGAG GGTCTAGTCA CAGACAAGAC ACTGTACAGT CACCAGTGTT TCCTCCCACC 
CAGAAACCTG TCCATCAAAA GCCTGTACCC TTGCCAAGGT TCCCAGAAGC GGGGAGCCCA 
GCTGCAGATG GACCGTTCCA CAGCTTCCCA TTTAATTCGA CGTTTGCAGA CCAGGAGGCT 
GAACTGCTCG GTAAGCCCTG GTATGCTGGC GCCTGTGACC GCAAGTCTGC TGAAGAGGCC 
TTGCACAGAT CCAACAAGGA TGGATCGTTT CTTATTCGGA AGAGCTCTGG CCATGATTCC 
AAGCAGCCGT ACACCCTAGT TGCGTTCTTT AACAAGCGAG TGTATAATAT TCCTGTACGG 
TTTATTGAAG CAACCAAACA GTATGCTTTG GGAAAGAAGA AAAATGGTGA AGAGTACTTC 
GGAAGTGTTG TGGAAATCGT CAACAGTCAC CAGCACAACC CCCTGGTTCT TATTGACAGT 
CAGAATAACA CGAAAGATTC CACGAGACTG AAATATGCTG TGAAGGTTTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032554.2
CDS	478..1851
Translation	MDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMDKIKKLKVKGPPSVPRRDYALDSPADEEEQWSDDFDSDYENPDEHSDSEMYVMPAEETGDDSYEPPPAEQQTRVVHPALPFTRGEYVDNRSSQRHSPPFSKTLPSKPSWPSAKARLASTLPAPNSLQKPQVPPKPKDLLEDEADYVVPVEDNDENYIHPRESSPPPAEKAPMVNRSTKPNSSSKHMSPPGTVAGRNSGVWDSKSSLPAAPSPLPRAGKKPATPLKTTPVPPLPNASNVCEEKPVPAERHRGSSHRQDTVQSPVFPPTQKPVHQKPVPLPRFPEAGSPAADGPFHSFPFNSTFADQEAELLGKPWYAGACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLVAFFNKRVYNIPVRFIEATKQYALGKKKNGEEYFGSVVEIVNSHQHNPLVLIDSQNNTKDSTRLKYAVKVS

Target ORF information:

RefSeq Version	NM_008528.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus B cell linker (Blnk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008528.4

ORF Insert Sequence:

ATGGACAAGC TGAATAAGAT AACTGTCCCT GCCAGCCAGA AGCTGAGACA GCTTCAAAAG 
ATGGTCCATG ATATTAAGAA CAATGAAGGT GGAATAATGG ACAAGATAAA AAAGCTAAAA 
GTCAAAGGCC CTCCAAGTGT TCCTCGAAGG GACTACGCAT TAGACAGCCC TGCAGATGAA 
GAGGAGCAGT GGTCAGATGA CTTCGACAGT GACTATGAAA ATCCAGATGA ACATTCGGAC 
TCCGAGATGT ATGTGATGCC TGCCGAGGAG ACGGGCGACG ATTCCTATGA ACCGCCTCCC 
GCTGAGCAGC AGACACGGGT GGTCCATCCA GCCCTGCCCT TCACGAGGGG CGAGTATGTA 
GATAATCGAT CCAGCCAGCG GCACTCTCCG CCCTTCAGCA AGACACTTCC CAGTAAGCCC 
AGCTGGCCTT CAGCGAAAGC GAGGCTGGCC TCCACTCTGC CAGCCCCCAA CTCTCTACAG 
AAGCCTCAAG TCCCCCCCAA GCCCAAAGAC CTCCTTGAGG ATGAGGCTGA TTATGTGGTC 
CCTGTGGAAG ATAACGATGA AAACTATATC CATCCCAGAG AAAGCAGCCC GCCGCCTGCT 
GAGAAGGCTC CCATGGTGAA TAGATCAACC AAGCCAAACA GTTCCTCAAA GCACATGTCG 
CCTCCAGGGA CTGTCGCAGG TCGAAACAGT GGGGTCTGGG ACTCCAAGTC ATCTTTGCCT 
GCCGCACCAT CCCCACTACC ACGGGCTGGG AAGAAGCCAG CTACACCACT TAAGACTACT 
CCCGTTCCTC CCCTACCGAA TGCATCAAAT GTTTGTGAAG AAAAGCCTGT TCCTGCTGAG 
CGCCACCGAG GGTCTAGTCA CAGACAAGAC ACTGTACAGT CACCAGTGTT TCCTCCCACC 
CAGAAACCTG TCCATCAAAA GCCTGTACCC TTGCCAAGGT TCCCAGAAGC GGGGAGCCCA 
GCTGCAGATG GACCGTTCCA CAGCTTCCCA TTTAATTCGA CGTTTGCAGA CCAGGAGGCT 
GAACTGCTCG GTAAGCCCTG GTATGCTGGC GCCTGTGACC GCAAGTCTGC TGAAGAGGCC 
TTGCACAGAT CCAACAAGGA TGGATCGTTT CTTATTCGGA AGAGCTCTGG CCATGATTCC 
AAGCAGCCGT ACACCCTAGT TGCGTTCTTT AACAAGCGAG TGTATAATAT TCCTGTACGG 
TTTATTGAAG CAACCAAACA GTATGCTTTG GGAAAGAAGA AAAATGGTGA AGAGTACTTC 
GGAAGTGTTG TGGAAATCGT CAACAGTCAC CAGCACAACC CCCTGGTTCT TATTGACAGT 
CAGAATAACA CGAAAGATTC CACGAGACTG AAATATGCTG TGAAGGTTTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blnk ( NM_001365054.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001351983.1 Mus musculus B cell linker (Blnk), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu84383
Clone ID Related Accession (Same CDS sequence)	NM_001365054.1
Accession Version	NM_001365054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	B-cell linker protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126608.20 and AC117253.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849380, SAMN00849382 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACAAGC TGAATAAGAT AACTGTCCCT GCCAGCCAGA AGCTGAGACA GCTTCAAAAG 
ATGGTCCATG ATATTAAGAA CAATGAAGGT GGAATAATGG ACAAGATAAA AAAGCTAAAA 
GTCAAAGGCC CTCCAAGTGT TCCTCGAAGG GACTACGCAT TAGACAGCCC TGCAGATGAA 
GAGGAGCAGT GGTCAGATGA CTTCGACAGT GACTATGAAA ATCCAGATGA ACATTCGGAC 
TCCGAGATGT ATGTGATGCC TGCCGAGGAG ACGGGCGACG ATTCCTATGA ACCGCCTCCC 
GCTGAGCAGC AGACACGGGT GGTCCATCCA GCCCTGCCCT TCACGAGGGG CGAGTATGTA 
GATAATCGAT CCAGCCAGCG GCACTCTCCG CCCTTCAGCA AGACACTTCC CAGTAAGCCC 
AGCTGGCCTT CAGCGAAAGC GAGGCTGGCC TCCACTCTGC CAGCCCCCAA CTCTCTACAG 
AAGCCTCAAG TCCCCCCCAA GCCCAAAGAC CTCCTTGAGG ATGAGGCTGA TTATGTGGTC 
CCTGTGGAAG ATAACGATGA AAACTATATC CATCCCAGAG AAAGCAGCCC GCCGCCTGCT 
GAGAAGGCTC CCATGGTGAA TAGATCAACC AAGCCAAACA GTTCCTCAAA GCACATGTCG 
CCTCCAGGGA CTGTCGCAGG TCGAAACAGT GGGGTCTGGG ACTCCAAGTC ATCTTTGCCT 
GCCGCACCAT CCCCACTACC ACGGGCTGGG AAGAAGCCAG CTACACCACT TAAGACTACT 
CCCGTTCCTC CCCTACCGAA TGCATCAAAT GTTTGTGAAG AAAAGCCTGT TCCTGCTGAG 
CGCCACCGAG GGTCTAGTCA CAGACAAGAC ACTGTACAGT CACCAGTGTT TCCTCCCACC 
CAGAAACCTG TCCATCAAAA GCCTGTACCC TTGCCAAAAG CGGGGAGCCC AGCTGCAGAT 
GGACCGTTCC ACAGCTTCCC ATTTAATTCG ACGTTTGCAG ACCAGGAGGC TGAACTGCTC 
GGTAAGCCCT GGTATGCTGG CGCCTGTGAC CGCAAGTCTG CTGAAGAGGC CTTGCACAGA 
TCCAACAAGG ATGGATCGTT TCTTATTCGG AAGAGCTCTG GCCATGATTC CAAGCAGCCG 
TACACCCTAG TTGCGTTCTT TAACAAGCGA GTGTATAATA TTCCTGTACG GTTTATTGAA 
GCAACCAAAC AGTATGCTTT GGGAAAGAAG AAAAATGGTG AAGAGTACTT CGGAAGTGTT 
GTGGAAATCG TCAACAGTCA CCAGCACAAC CCCCTGGTTC TTATTGACAG TCAGAATAAC 
ACGAAAGATT CCACGAGACT GAAATATGCT GTGAAGGTTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351983.1
CDS	164..1528
Translation	MDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMDKIKKLKVKGPPSVPRRDYALDSPADEEEQWSDDFDSDYENPDEHSDSEMYVMPAEETGDDSYEPPPAEQQTRVVHPALPFTRGEYVDNRSSQRHSPPFSKTLPSKPSWPSAKARLASTLPAPNSLQKPQVPPKPKDLLEDEADYVVPVEDNDENYIHPRESSPPPAEKAPMVNRSTKPNSSSKHMSPPGTVAGRNSGVWDSKSSLPAAPSPLPRAGKKPATPLKTTPVPPLPNASNVCEEKPVPAERHRGSSHRQDTVQSPVFPPTQKPVHQKPVPLPKAGSPAADGPFHSFPFNSTFADQEAELLGKPWYAGACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLVAFFNKRVYNIPVRFIEATKQYALGKKKNGEEYFGSVVEIVNSHQHNPLVLIDSQNNTKDSTRLKYAVKVS

Target ORF information:

RefSeq Version	NM_001365054.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus B cell linker (Blnk), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365054.1

ORF Insert Sequence:

ATGGACAAGC TGAATAAGAT AACTGTCCCT GCCAGCCAGA AGCTGAGACA GCTTCAAAAG 
ATGGTCCATG ATATTAAGAA CAATGAAGGT GGAATAATGG ACAAGATAAA AAAGCTAAAA 
GTCAAAGGCC CTCCAAGTGT TCCTCGAAGG GACTACGCAT TAGACAGCCC TGCAGATGAA 
GAGGAGCAGT GGTCAGATGA CTTCGACAGT GACTATGAAA ATCCAGATGA ACATTCGGAC 
TCCGAGATGT ATGTGATGCC TGCCGAGGAG ACGGGCGACG ATTCCTATGA ACCGCCTCCC 
GCTGAGCAGC AGACACGGGT GGTCCATCCA GCCCTGCCCT TCACGAGGGG CGAGTATGTA 
GATAATCGAT CCAGCCAGCG GCACTCTCCG CCCTTCAGCA AGACACTTCC CAGTAAGCCC 
AGCTGGCCTT CAGCGAAAGC GAGGCTGGCC TCCACTCTGC CAGCCCCCAA CTCTCTACAG 
AAGCCTCAAG TCCCCCCCAA GCCCAAAGAC CTCCTTGAGG ATGAGGCTGA TTATGTGGTC 
CCTGTGGAAG ATAACGATGA AAACTATATC CATCCCAGAG AAAGCAGCCC GCCGCCTGCT 
GAGAAGGCTC CCATGGTGAA TAGATCAACC AAGCCAAACA GTTCCTCAAA GCACATGTCG 
CCTCCAGGGA CTGTCGCAGG TCGAAACAGT GGGGTCTGGG ACTCCAAGTC ATCTTTGCCT 
GCCGCACCAT CCCCACTACC ACGGGCTGGG AAGAAGCCAG CTACACCACT TAAGACTACT 
CCCGTTCCTC CCCTACCGAA TGCATCAAAT GTTTGTGAAG AAAAGCCTGT TCCTGCTGAG 
CGCCACCGAG GGTCTAGTCA CAGACAAGAC ACTGTACAGT CACCAGTGTT TCCTCCCACC 
CAGAAACCTG TCCATCAAAA GCCTGTACCC TTGCCAAAAG CGGGGAGCCC AGCTGCAGAT 
GGACCGTTCC ACAGCTTCCC ATTTAATTCG ACGTTTGCAG ACCAGGAGGC TGAACTGCTC 
GGTAAGCCCT GGTATGCTGG CGCCTGTGAC CGCAAGTCTG CTGAAGAGGC CTTGCACAGA 
TCCAACAAGG ATGGATCGTT TCTTATTCGG AAGAGCTCTG GCCATGATTC CAAGCAGCCG 
TACACCCTAG TTGCGTTCTT TAACAAGCGA GTGTATAATA TTCCTGTACG GTTTATTGAA 
GCAACCAAAC AGTATGCTTT GGGAAAGAAG AAAAATGGTG AAGAGTACTT CGGAAGTGTT 
GTGGAAATCG TCAACAGTCA CCAGCACAAC CCCCTGGTTC TTATTGACAG TCAGAATAAC 
ACGAAAGATT CCACGAGACT GAAATATGCT GTGAAGGTTT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The BTG2-PRMT1 module limits pre-B cell expansion by regulating the CDK4-Cyclin-D3 complex.
Nature immunology18(8)911-920(2017 Aug)
Dolezal E,Infantino S,Drepper F,B?rsig T,Singh A,Wossning T,Fiala GJ,Minguet S,Warscheid B,Tarlinton DM,Jumaa H,Medgyesi D,Reth M

Development of SH2 probes and pull-down assays to detect pathogen-induced, site-specific tyrosine phosphorylation of the TLR adaptor SCIMP.
Immunology and cell biology95(6)564-570(2017 Jul)
Luo L,Tong SJ,Wall AA,Khromykh T,Sweet MJ,Stow JL

Regulation of B cell fate by chronic activity of the IgE B cell receptor.
eLife5(2016 12)
Yang Z,Robinson MJ,Chen X,Smith GA,Taunton J,Liu W,Allen CD

Autonomous membrane IgE signaling prevents IgE-memory formation.
Nature immunology17(9)1109-17(2016 09)
Haniuda K,Fukao S,Kodama T,Hasegawa H,Kitamura D

B Cell Linker Protein (BLNK) Is a Selective Target of Repression by PAX5-PML Protein in the Differentiation Block That Leads to the Development of Acute Lymphoblastic Leukemia.
The Journal of biological chemistry291(9)4723-31(2016 Feb)
Imoto N,Hayakawa F,Kurahashi S,Morishita T,Kojima Y,Yasuda T,Sugimoto K,Tsuzuki S,Naoe T,Kiyoi H

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Blnk cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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