Nphs2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nphs2
Entrez Gene ID	170484
Full Name	nephrosis 2, podocin
Synonyms	PDCN,SRN1
General protein information	Preferred Names nephrosis 2, podocin Names podocin nephrosis 2 homolog, podocin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 G3

mRNA and Protein(s)

mRNA	Protein	Name
NM_130456.3	NP_569723.1	podocin
XM_006496684.3	XP_006496747.1	podocin isoform X2
NM_130456.4	NP_569723.1	podocin
XM_006496683.3	XP_006496746.1	podocin isoform X1
XM_011238765.2	XP_011237067.1	podocin isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NPHS2	XP_005623019.1
Homo sapiens (human)	NPHS2	NP_055440.1
Pan troglodytes (chimpanzee)	NPHS2	XP_003308663.2
Bos taurus (cattle)	NPHS2	NP_001193036.1
Gallus gallus (chicken)	NPHS2	XP_422265.3
Mus musculus (house mouse)	Nphs2	NP_569723.1
Rattus norvegicus (Norway rat)	Nphs2	NP_570841.2
	NPHS2	XP_002802082.1
Danio rerio (zebrafish)	nphs2	NP_001018155.1
Xenopus tropicalis (tropical clawed frog)	nphs2	XP_002937013.2

Gene Ontology
Bibliography

Related articles in PubMed

Notch is required for the formation of all nephron segments and primes nephron progenitors for differentiation.
Chung E, Deacon P, Park JS
Development (Cambridge, England)144(24)4530-4539(2017 Dec)

The FERM protein EPB41L5 regulates actomyosin contractility and focal adhesion formation to maintain the kidney filtration barrier.
Schell C, Rogg M, Suhm M, Helmst?dter M, Sellung D, Yasuda-Yamahara M, Kretz O, K?ttner V, Suleiman H, Kollipara L, Zahedi RP, Sickmann A, Eimer S, Shaw AS, Kramer-Zucker A, Hirano-Kobayashi M, Abe T, Aizawa S, Grahammer F, Hartleben B, Dengjel J, Huber TB
Proceedings of the National Academy of Sciences of the United States of America114(23)E4621-E4630(2017 Jun)

Developmental vascular remodeling defects and postnatal kidney failure in mice lacking Gpr116 (Adgrf5) and Eltd1 (Adgrl4).
Lu S, Liu S, Wietelmann A, Kojonazarov B, Atzberger A, Tang C, Schermuly RT, Gr?ne HJ, Offermanns S
PloS one12(8)e0183166(2017)

An inducible mouse model of podocin-mutation-related nephrotic syndrome.
Tabatabaeifar M, Wlodkowski T, Simic I, Denc H, Mollet G, Weber S, Moyers JJ, Br?hl B, Randles MJ, Lennon R, Antignac C, Schaefer F
PloS one12(10)e0186574(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

While R140Q podocin mRNA expression was elevated, protein abundance was reduced by more than 50% within one week following induction
Title: An inducible mouse model of podocin-mutation-related nephrotic syndrome.

Ubiquitin ligase Ubr4 is a key component of the podocin interactome purified from cultured podocytes and native glomeruli. Ubiquitylomic analysis of mouse glomeruli revealed that podocin is ubiquitylated at two lysine residues. Ubiquitylation of one podocin site, K301, do not only target podocin for proteasomal degradation, but may also affect stability and disassembly of the multimeric complex.
Title: The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes.

ATP evokes activation of TRPC6 channels in podocytes through P2Y receptors, podocin, and generation of reactive oxygen species.
Title: ATP acting through P2Y receptors causes activation of podocyte TRPC6 channels: role of podocin and reactive oxygen species.

the carboxyl terminus of podocin/MEC-2 has to be placed at the inner leaflet of the plasma membrane to mediate cholesterol binding and contribute to ion channel activity.
Title: A disease-causing mutation illuminates the protein membrane topology of the kidney-expressed prohibitin homology (PHB) domain protein podocin.

Podocin is necessary for assembly of the NOX2-TRPC6 complex in lipid rafts.
Title: NOX2 interacts with podocyte TRPC6 channels and contributes to their activation by diacylglycerol: essential role of podocin in formation of this complex.

The following Nphs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nphs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu28642	XM_006496683.3 Latest version!	Mus musculus nephrosis 2, podocin (Nphs2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu28643	XM_006496684.3 Latest version!	Mus musculus nephrosis 2, podocin (Nphs2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu72491	XM_011238765.2 Latest version!	Mus musculus nephrosis 2, podocin (Nphs2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu09785	NM_130456.4 Latest version!	Mus musculus nephrosis 2, podocin (Nphs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu09785	NM_130456.3	Mus musculus nephrosis 2, podocin (Nphs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nphs2 ( XM_006496683.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496746.1 Mus musculus nephrosis 2, podocin (Nphs2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28642
Clone ID Related Accession (Same CDS sequence)	XM_006496683.3
Accession Version	XM_006496683.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	podocin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496683.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCA GGGCGCGGAG CTCTTCCCGA GAGGCCCACG GGAGAAGTAG CAGGTCCTCC 
TCTAGGGATG ACAAGAAGGC AAAGGCCGGG AGGGGCAGCA GAGGCCGCGC ACGTCCGGAT 
GCTGGAGCAG AGCGGCAGAG CACCGGGCGG ACAGCGACCC GAGGGGAGCC CCGAGCTCCC 
GCTGCCACCG CCACCGTAGT GGACGTGGAC GAGGTTCGGG GCCCTGGTGA GGAGGGCACG 
GAAGTGGTGG CGCTGCTGGA GAGCGAGCGA CCAGAGGAAG GCATCAAGCC CTCTGGATTA 
GGGGCCTGCG AGTGGCTTCT TGTCCTCGCC TCCCTGATCT TCATCATCAT GACGTTCCCT 
TTTTCCATCT GGTTCTGCAT AAAGGTTGTG CAAGAGTATG AAAGAGTAAT TATATTCCGA 
CTGGGACATC TGCTTCCTGG AAGAGCCAAA GGTCCTGGCC TGTTCTTTTT TTTACCCTGT 
CTGGACACCT ATCACAAGGT TGATCTCCGT CTCCAGACCT TGGAAATACC TTTCCATGAG 
GTGGTAACCA AAGATATGTT TATAATGGAG ATAGACGCTG TCTGCTACTA CCGCATGGAA 
AATGCCTCTC TTCTTCTAAG CAGTCTAGCC CATGTGTCCA AAGCCATCCA GTTCCTGGTG 
CAAACCACCA TGAAGCGCCT CTTGGCACAT CGATCCCTCA CTGAAATTCT CCTGGAAAGG 
AAGAGCATTG CCCAAGATGT AAAGGTTGCC TTAGATGCAG TGACCTGTAT TTGGGGCATC 
AAAGTGGAGA GAACTGAAAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496746.1
CDS	241..1044
Translation	MDSRARSSSREAHGRSSRSSSRDDKKAKAGRGSRGRARPDAGAERQSTGRTATRGEPRAPAATATVVDVDEVRGPGEEGTEVVALLESERPEEGIKPSGLGACEWLLVLASLIFIIMTFPFSIWFCIKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLDTYHKVDLRLQTLEIPFHEVVTKDMFIMEIDAVCYYRMENASLLLSSLAHVSKAIQFLVQTTMKRLLAHRSLTEILLERKSIAQDVKVALDAVTCIWGIKVERTEM

Target ORF information:

RefSeq Version	XM_006496683.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephrosis 2, podocin (Nphs2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496683.3

ORF Insert Sequence:

ATGGACAGCA GGGCGCGGAG CTCTTCCCGA GAGGCCCACG GGAGAAGTAG CAGGTCCTCC 
TCTAGGGATG ACAAGAAGGC AAAGGCCGGG AGGGGCAGCA GAGGCCGCGC ACGTCCGGAT 
GCTGGAGCAG AGCGGCAGAG CACCGGGCGG ACAGCGACCC GAGGGGAGCC CCGAGCTCCC 
GCTGCCACCG CCACCGTAGT GGACGTGGAC GAGGTTCGGG GCCCTGGTGA GGAGGGCACG 
GAAGTGGTGG CGCTGCTGGA GAGCGAGCGA CCAGAGGAAG GCATCAAGCC CTCTGGATTA 
GGGGCCTGCG AGTGGCTTCT TGTCCTCGCC TCCCTGATCT TCATCATCAT GACGTTCCCT 
TTTTCCATCT GGTTCTGCAT AAAGGTTGTG CAAGAGTATG AAAGAGTAAT TATATTCCGA 
CTGGGACATC TGCTTCCTGG AAGAGCCAAA GGTCCTGGCC TGTTCTTTTT TTTACCCTGT 
CTGGACACCT ATCACAAGGT TGATCTCCGT CTCCAGACCT TGGAAATACC TTTCCATGAG 
GTGGTAACCA AAGATATGTT TATAATGGAG ATAGACGCTG TCTGCTACTA CCGCATGGAA 
AATGCCTCTC TTCTTCTAAG CAGTCTAGCC CATGTGTCCA AAGCCATCCA GTTCCTGGTG 
CAAACCACCA TGAAGCGCCT CTTGGCACAT CGATCCCTCA CTGAAATTCT CCTGGAAAGG 
AAGAGCATTG CCCAAGATGT AAAGGTTGCC TTAGATGCAG TGACCTGTAT TTGGGGCATC 
AAAGTGGAGA GAACTGAAAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nphs2 ( XM_006496684.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496747.1 Mus musculus nephrosis 2, podocin (Nphs2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu28643
Clone ID Related Accession (Same CDS sequence)	XM_006496684.3
Accession Version	XM_006496684.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	podocin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTTC CCTCCCGCCC TTTTGTAGGC CTGTTCTTTT TTTTACCCTG TCTGGACACC 
TATCACAAGG TTGATCTCCG TCTCCAGACC TTGGAAATAC CTTTCCATGA GGTGGTAACC 
AAAGATATGT TTATAATGGA GATAGACGCT GTCTGCTACT ACCGCATGGA AAATGCCTCT 
CTTCTTCTAA GCAGTCTAGC CCATGTGTCC AAAGCCATCC AGTTCCTGGT GCAAACCACC 
ATGAAGCGCC TCTTGGCACA TCGATCCCTC ACTGAAATTC TCCTGGAAAG GAAGAGCATT 
GCCCAAGATG TAAAGGTTGC CTTAGATGCA GTGACCTGTA TTTGGGGCAT CAAAGTGGAG 
AGAACTGAAA TTAAGGATGT GAGGCTGCCA GCTGGGCTTC AGCACTCTCT GGCGGTGGAA 
GCTGAGGCAC AAAGACAGGC CAAAGTGCGG GTGATTGCTG CAGAAGGGGA AAAGGCTGCT 
TCAGAGTCCC TGAGGATGGC GGCTGAGATT CTGTCGGGCA CCCCAGCCGC TGTCCAGCTT 
CGATACTTGC ACACTCTTCA GTCGCTGTCC ACAGAGAAGC CAGCCACTGT GGTTTTGCCT 
TTGCCATTTG ACATGCTAAG CCTTTTGTCC TCTCCCGGCA ACAGAGCACA AGGGAGCATC 
AACTACCCAA GTTCCTCCAA ACCAGTTGAA CCACTAAATC CCAAAAAGAA GGACTCTCCT 
ATGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496747.1
CDS	210..938
Translation	MVLPSRPFVGLFFFLPCLDTYHKVDLRLQTLEIPFHEVVTKDMFIMEIDAVCYYRMENASLLLSSLAHVSKAIQFLVQTTMKRLLAHRSLTEILLERKSIAQDVKVALDAVTCIWGIKVERTEIKDVRLPAGLQHSLAVEAEAQRQAKVRVIAAEGEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPATVVLPLPFDMLSLLSSPGNRAQGSINYPSSSKPVEPLNPKKKDSPML

Target ORF information:

RefSeq Version	XM_006496684.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephrosis 2, podocin (Nphs2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496684.3

ORF Insert Sequence:

ATGGTCCTTC CCTCCCGCCC TTTTGTAGGC CTGTTCTTTT TTTTACCCTG TCTGGACACC 
TATCACAAGG TTGATCTCCG TCTCCAGACC TTGGAAATAC CTTTCCATGA GGTGGTAACC 
AAAGATATGT TTATAATGGA GATAGACGCT GTCTGCTACT ACCGCATGGA AAATGCCTCT 
CTTCTTCTAA GCAGTCTAGC CCATGTGTCC AAAGCCATCC AGTTCCTGGT GCAAACCACC 
ATGAAGCGCC TCTTGGCACA TCGATCCCTC ACTGAAATTC TCCTGGAAAG GAAGAGCATT 
GCCCAAGATG TAAAGGTTGC CTTAGATGCA GTGACCTGTA TTTGGGGCAT CAAAGTGGAG 
AGAACTGAAA TTAAGGATGT GAGGCTGCCA GCTGGGCTTC AGCACTCTCT GGCGGTGGAA 
GCTGAGGCAC AAAGACAGGC CAAAGTGCGG GTGATTGCTG CAGAAGGGGA AAAGGCTGCT 
TCAGAGTCCC TGAGGATGGC GGCTGAGATT CTGTCGGGCA CCCCAGCCGC TGTCCAGCTT 
CGATACTTGC ACACTCTTCA GTCGCTGTCC ACAGAGAAGC CAGCCACTGT GGTTTTGCCT 
TTGCCATTTG ACATGCTAAG CCTTTTGTCC TCTCCCGGCA ACAGAGCACA AGGGAGCATC 
AACTACCCAA GTTCCTCCAA ACCAGTTGAA CCACTAAATC CCAAAAAGAA GGACTCTCCT 
ATGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nphs2 ( XM_011238765.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237067.1 Mus musculus nephrosis 2, podocin (Nphs2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu72491
Clone ID Related Accession (Same CDS sequence)	XM_011238765.2
Accession Version	XM_011238765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	podocin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCA GGGCGCGGAG CTCTTCCCGA GAGGCCCACG GGAGAAGTAG CAGGTCCTCC 
TCTAGGGATG ACAAGAAGGC AAAGGCCGGG AGGGGCAGCA GAGGCCGCGC ACGTCCGGAT 
GCTGGAGCAG AGCGGCAGAG CACCGGGCGG ACAGCGACCC GAGGGGAGCC CCGAGCTCCC 
GCTGCCACCG CCACCGTAGT GGACGTGGAC GAGGTTCGGG GCCCTGGTGA GGAGGGCACG 
GAAGTGGTGG CGCTGCTGGA GAGCGAGCGA CCAGAGGAAG GCATCAAGCC CTCTGGATTA 
GGGGCCTGCG AGTGGCTTCT TGTCCTCGCC TCCCTGATCT TCATCATCAT GACGTTCCCT 
TTTTCCATCT GGTTCTGCAT AAAGGTTGTG CAAGAGTATG AAAGAGTAAT TATATTCCGA 
CTGGGACATC TGCTTCCTGG AAGAGCCAAA GGTCCTGAAC CATCCCCAGT CGAATATGAA 
TTACAGGACA ATACTTTAGT TACAAGGAAG GTGCCCAGTG GTCTCTGGGT AACACAGGCT 
AAGCCAGCAA TGCAGCCTGG CTCTGAAAAG CTCATCTCCA GCAGCCTTCA CACTTGCTGT 
TCACATGGTC CTTCCCTCCC GCCCTTTTGT AGGCCTGTTC TTTTTTTTAC CCTGTCTGGA 
CACCTATCAC AAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237067.1
CDS	254..931
Translation	MDSRARSSSREAHGRSSRSSSRDDKKAKAGRGSRGRARPDAGAERQSTGRTATRGEPRAPAATATVVDVDEVRGPGEEGTEVVALLESERPEEGIKPSGLGACEWLLVLASLIFIIMTFPFSIWFCIKVVQEYERVIIFRLGHLLPGRAKGPEPSPVEYELQDNTLVTRKVPSGLWVTQAKPAMQPGSEKLISSSLHTCCSHGPSLPPFCRPVLFFTLSGHLSQG

Target ORF information:

RefSeq Version	XM_011238765.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephrosis 2, podocin (Nphs2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238765.2

ORF Insert Sequence:

ATGGACAGCA GGGCGCGGAG CTCTTCCCGA GAGGCCCACG GGAGAAGTAG CAGGTCCTCC 
TCTAGGGATG ACAAGAAGGC AAAGGCCGGG AGGGGCAGCA GAGGCCGCGC ACGTCCGGAT 
GCTGGAGCAG AGCGGCAGAG CACCGGGCGG ACAGCGACCC GAGGGGAGCC CCGAGCTCCC 
GCTGCCACCG CCACCGTAGT GGACGTGGAC GAGGTTCGGG GCCCTGGTGA GGAGGGCACG 
GAAGTGGTGG CGCTGCTGGA GAGCGAGCGA CCAGAGGAAG GCATCAAGCC CTCTGGATTA 
GGGGCCTGCG AGTGGCTTCT TGTCCTCGCC TCCCTGATCT TCATCATCAT GACGTTCCCT 
TTTTCCATCT GGTTCTGCAT AAAGGTTGTG CAAGAGTATG AAAGAGTAAT TATATTCCGA 
CTGGGACATC TGCTTCCTGG AAGAGCCAAA GGTCCTGAAC CATCCCCAGT CGAATATGAA 
TTACAGGACA ATACTTTAGT TACAAGGAAG GTGCCCAGTG GTCTCTGGGT AACACAGGCT 
AAGCCAGCAA TGCAGCCTGG CTCTGAAAAG CTCATCTCCA GCAGCCTTCA CACTTGCTGT 
TCACATGGTC CTTCCCTCCC GCCCTTTTGT AGGCCTGTTC TTTTTTTTAC CCTGTCTGGA 
CACCTATCAC AAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nphs2 ( NM_130456.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_569723.1 Mus musculus nephrosis 2, podocin (Nphs2), mRNA.

Price & Availability↑

CloneID	OMu09785
Clone ID Related Accession (Same CDS sequence)	NM_130456.3 , NM_130456.4
Accession Version	NM_130456.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	podocin
Comment

ATGGACAGCA GGGCGCGGAG CTCTTCCCGA GAGGCCCACG GGAGAAGTAG CAGGTCCTCC 
TCTAGGGATG ACAAGAAGGC AAAGGCCGGG AGGGGCAGCA GAGGCCGCGC ACGTCCGGAT 
GCTGGAGCAG AGCGGCAGAG CACCGGGCGG ACAGCGACCC GAGGGGAGCC CCGAGCTCCC 
GCTGCCACCG CCACCGTAGT GGACGTGGAC GAGGTTCGGG GCCCTGGTGA GGAGGGCACG 
GAAGTGGTGG CGCTGCTGGA GAGCGAGCGA CCAGAGGAAG GCATCAAGCC CTCTGGATTA 
GGGGCCTGCG AGTGGCTTCT TGTCCTCGCC TCCCTGATCT TCATCATCAT GACGTTCCCT 
TTTTCCATCT GGTTCTGCAT AAAGGTTGTG CAAGAGTATG AAAGAGTAAT TATATTCCGA 
CTGGGACATC TGCTTCCTGG AAGAGCCAAA GGTCCTGGCC TGTTCTTTTT TTTACCCTGT 
CTGGACACCT ATCACAAGGT TGATCTCCGT CTCCAGACCT TGGAAATACC TTTCCATGAG 
GTGGTAACCA AAGATATGTT TATAATGGAG ATAGACGCTG TCTGCTACTA CCGCATGGAA 
AATGCCTCTC TTCTTCTAAG CAGTCTAGCC CATGTGTCCA AAGCCATCCA GTTCCTGGTG 
CAAACCACCA TGAAGCGCCT CTTGGCACAT CGATCCCTCA CTGAAATTCT CCTGGAAAGG 
AAGAGCATTG CCCAAGATGT AAAGGTTGCC TTAGATGCAG TGACCTGTAT TTGGGGCATC 
AAAGTGGAGA GAACTGAAAT TAAGGATGTG AGGCTGCCAG CTGGGCTTCA GCACTCTCTG 
GCGGTGGAAG CTGAGGCACA AAGACAGGCC AAAGTGCGGG TGATTGCTGC AGAAGGGGAA 
AAGGCTGCTT CAGAGTCCCT GAGGATGGCG GCTGAGATTC TGTCGGGCAC CCCAGCCGCT 
GTCCAGCTTC GATACTTGCA CACTCTTCAG TCGCTGTCCA CAGAGAAGCC AGCCACTGTG 
GTTTTGCCTT TGCCATTTGA CATGCTAAGC CTTTTGTCCT CTCCCGGCAA CAGAGCACAA 
GGGAGCATCA ACTACCCAAG TTCCTCCAAA CCAGTTGAAC CACTAAATCC CAAAAAGAAG 
GACTCTCCTA TGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569723.1
Translation	MDSRARSSSREAHGRSSRSSSRDDKKAKAGRGSRGRARPDAGAERQSTGRTATRGEPRAPAATATVVDVDEVRGPGEEGTEVVALLESERPEEGIKPSGLGACEWLLVLASLIFIIMTFPFSIWFCIKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLDTYHKVDLRLQTLEIPFHEVVTKDMFIMEIDAVCYYRMENASLLLSSLAHVSKAIQFLVQTTMKRLLAHRSLTEILLERKSIAQDVKVALDAVTCIWGIKVERTEIKDVRLPAGLQHSLAVEAEAQRQAKVRVIAAEGEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPATVVLPLPFDMLSLLSSPGNRAQGSINYPSSSKPVEPLNPKKKDSPML

Target ORF information:

RefSeq Version	NM_130456.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus nephrosis 2, podocin (Nphs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130456.3

ORF Insert Sequence:

ATGGACAGCA GGGCGCGGAG CTCTTCCCGA GAGGCCCACG GGAGAAGTAG CAGGTCCTCC 
TCTAGGGATG ACAAGAAGGC AAAGGCCGGG AGGGGCAGCA GAGGCCGCGC ACGTCCGGAT 
GCTGGAGCAG AGCGGCAGAG CACCGGGCGG ACAGCGACCC GAGGGGAGCC CCGAGCTCCC 
GCTGCCACCG CCACCGTAGT GGACGTGGAC GAGGTTCGGG GCCCTGGTGA GGAGGGCACG 
GAAGTGGTGG CGCTGCTGGA GAGCGAGCGA CCAGAGGAAG GCATCAAGCC CTCTGGATTA 
GGGGCCTGCG AGTGGCTTCT TGTCCTCGCC TCCCTGATCT TCATCATCAT GACGTTCCCT 
TTTTCCATCT GGTTCTGCAT AAAGGTTGTG CAAGAGTATG AAAGAGTAAT TATATTCCGA 
CTGGGACATC TGCTTCCTGG AAGAGCCAAA GGTCCTGGCC TGTTCTTTTT TTTACCCTGT 
CTGGACACCT ATCACAAGGT TGATCTCCGT CTCCAGACCT TGGAAATACC TTTCCATGAG 
GTGGTAACCA AAGATATGTT TATAATGGAG ATAGACGCTG TCTGCTACTA CCGCATGGAA 
AATGCCTCTC TTCTTCTAAG CAGTCTAGCC CATGTGTCCA AAGCCATCCA GTTCCTGGTG 
CAAACCACCA TGAAGCGCCT CTTGGCACAT CGATCCCTCA CTGAAATTCT CCTGGAAAGG 
AAGAGCATTG CCCAAGATGT AAAGGTTGCC TTAGATGCAG TGACCTGTAT TTGGGGCATC 
AAAGTGGAGA GAACTGAAAT TAAGGATGTG AGGCTGCCAG CTGGGCTTCA GCACTCTCTG 
GCGGTGGAAG CTGAGGCACA AAGACAGGCC AAAGTGCGGG TGATTGCTGC AGAAGGGGAA 
AAGGCTGCTT CAGAGTCCCT GAGGATGGCG GCTGAGATTC TGTCGGGCAC CCCAGCCGCT 
GTCCAGCTTC GATACTTGCA CACTCTTCAG TCGCTGTCCA CAGAGAAGCC AGCCACTGTG 
GTTTTGCCTT TGCCATTTGA CATGCTAAGC CTTTTGTCCT CTCCCGGCAA CAGAGCACAA 
GGGAGCATCA ACTACCCAAG TTCCTCCAAA CCAGTTGAAC CACTAAATCC CAAAAAGAAG 
GACTCTCCTA TGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nphs2 ( NM_130456.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_569723.1 Mus musculus nephrosis 2, podocin (Nphs2), mRNA.

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CloneID	OMu09785
Clone ID Related Accession (Same CDS sequence)	NM_130456.3 , NM_130456.4
Accession Version	NM_130456.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	podocin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161414.3 and FJ715770.1. On Sep 1, 2015 this sequence version replaced NM_130456.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ715770.1, AY050309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACAGCA GGGCGCGGAG CTCTTCCCGA GAGGCCCACG GGAGAAGTAG CAGGTCCTCC 
TCTAGGGATG ACAAGAAGGC AAAGGCCGGG AGGGGCAGCA GAGGCCGCGC ACGTCCGGAT 
GCTGGAGCAG AGCGGCAGAG CACCGGGCGG ACAGCGACCC GAGGGGAGCC CCGAGCTCCC 
GCTGCCACCG CCACCGTAGT GGACGTGGAC GAGGTTCGGG GCCCTGGTGA GGAGGGCACG 
GAAGTGGTGG CGCTGCTGGA GAGCGAGCGA CCAGAGGAAG GCATCAAGCC CTCTGGATTA 
GGGGCCTGCG AGTGGCTTCT TGTCCTCGCC TCCCTGATCT TCATCATCAT GACGTTCCCT 
TTTTCCATCT GGTTCTGCAT AAAGGTTGTG CAAGAGTATG AAAGAGTAAT TATATTCCGA 
CTGGGACATC TGCTTCCTGG AAGAGCCAAA GGTCCTGGCC TGTTCTTTTT TTTACCCTGT 
CTGGACACCT ATCACAAGGT TGATCTCCGT CTCCAGACCT TGGAAATACC TTTCCATGAG 
GTGGTAACCA AAGATATGTT TATAATGGAG ATAGACGCTG TCTGCTACTA CCGCATGGAA 
AATGCCTCTC TTCTTCTAAG CAGTCTAGCC CATGTGTCCA AAGCCATCCA GTTCCTGGTG 
CAAACCACCA TGAAGCGCCT CTTGGCACAT CGATCCCTCA CTGAAATTCT CCTGGAAAGG 
AAGAGCATTG CCCAAGATGT AAAGGTTGCC TTAGATGCAG TGACCTGTAT TTGGGGCATC 
AAAGTGGAGA GAACTGAAAT TAAGGATGTG AGGCTGCCAG CTGGGCTTCA GCACTCTCTG 
GCGGTGGAAG CTGAGGCACA AAGACAGGCC AAAGTGCGGG TGATTGCTGC AGAAGGGGAA 
AAGGCTGCTT CAGAGTCCCT GAGGATGGCG GCTGAGATTC TGTCGGGCAC CCCAGCCGCT 
GTCCAGCTTC GATACTTGCA CACTCTTCAG TCGCTGTCCA CAGAGAAGCC AGCCACTGTG 
GTTTTGCCTT TGCCATTTGA CATGCTAAGC CTTTTGTCCT CTCCCGGCAA CAGAGCACAA 
GGGAGCATCA ACTACCCAAG TTCCTCCAAA CCAGTTGAAC CACTAAATCC CAAAAAGAAG 
GACTCTCCTA TGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569723.1
CDS	70..1227
Translation	MDSRARSSSREAHGRSSRSSSRDDKKAKAGRGSRGRARPDAGAERQSTGRTATRGEPRAPAATATVVDVDEVRGPGEEGTEVVALLESERPEEGIKPSGLGACEWLLVLASLIFIIMTFPFSIWFCIKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLDTYHKVDLRLQTLEIPFHEVVTKDMFIMEIDAVCYYRMENASLLLSSLAHVSKAIQFLVQTTMKRLLAHRSLTEILLERKSIAQDVKVALDAVTCIWGIKVERTEIKDVRLPAGLQHSLAVEAEAQRQAKVRVIAAEGEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPATVVLPLPFDMLSLLSSPGNRAQGSINYPSSSKPVEPLNPKKKDSPML

Target ORF information:

RefSeq Version	NM_130456.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephrosis 2, podocin (Nphs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130456.4

ORF Insert Sequence:

ATGGACAGCA GGGCGCGGAG CTCTTCCCGA GAGGCCCACG GGAGAAGTAG CAGGTCCTCC 
TCTAGGGATG ACAAGAAGGC AAAGGCCGGG AGGGGCAGCA GAGGCCGCGC ACGTCCGGAT 
GCTGGAGCAG AGCGGCAGAG CACCGGGCGG ACAGCGACCC GAGGGGAGCC CCGAGCTCCC 
GCTGCCACCG CCACCGTAGT GGACGTGGAC GAGGTTCGGG GCCCTGGTGA GGAGGGCACG 
GAAGTGGTGG CGCTGCTGGA GAGCGAGCGA CCAGAGGAAG GCATCAAGCC CTCTGGATTA 
GGGGCCTGCG AGTGGCTTCT TGTCCTCGCC TCCCTGATCT TCATCATCAT GACGTTCCCT 
TTTTCCATCT GGTTCTGCAT AAAGGTTGTG CAAGAGTATG AAAGAGTAAT TATATTCCGA 
CTGGGACATC TGCTTCCTGG AAGAGCCAAA GGTCCTGGCC TGTTCTTTTT TTTACCCTGT 
CTGGACACCT ATCACAAGGT TGATCTCCGT CTCCAGACCT TGGAAATACC TTTCCATGAG 
GTGGTAACCA AAGATATGTT TATAATGGAG ATAGACGCTG TCTGCTACTA CCGCATGGAA 
AATGCCTCTC TTCTTCTAAG CAGTCTAGCC CATGTGTCCA AAGCCATCCA GTTCCTGGTG 
CAAACCACCA TGAAGCGCCT CTTGGCACAT CGATCCCTCA CTGAAATTCT CCTGGAAAGG 
AAGAGCATTG CCCAAGATGT AAAGGTTGCC TTAGATGCAG TGACCTGTAT TTGGGGCATC 
AAAGTGGAGA GAACTGAAAT TAAGGATGTG AGGCTGCCAG CTGGGCTTCA GCACTCTCTG 
GCGGTGGAAG CTGAGGCACA AAGACAGGCC AAAGTGCGGG TGATTGCTGC AGAAGGGGAA 
AAGGCTGCTT CAGAGTCCCT GAGGATGGCG GCTGAGATTC TGTCGGGCAC CCCAGCCGCT 
GTCCAGCTTC GATACTTGCA CACTCTTCAG TCGCTGTCCA CAGAGAAGCC AGCCACTGTG 
GTTTTGCCTT TGCCATTTGA CATGCTAAGC CTTTTGTCCT CTCCCGGCAA CAGAGCACAA 
GGGAGCATCA ACTACCCAAG TTCCTCCAAA CCAGTTGAAC CACTAAATCC CAAAAAGAAG 
GACTCTCCTA TGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Notch is required for the formation of all nephron segments and primes nephron progenitors for differentiation.
Development (Cambridge, England)144(24)4530-4539(2017 Dec)
Chung E,Deacon P,Park JS

The FERM protein EPB41L5 regulates actomyosin contractility and focal adhesion formation to maintain the kidney filtration barrier.
Proceedings of the National Academy of Sciences of the United States of America114(23)E4621-E4630(2017 Jun)
Schell C,Rogg M,Suhm M,Helmst?dter M,Sellung D,Yasuda-Yamahara M,Kretz O,K?ttner V,Suleiman H,Kollipara L,Zahedi RP,Sickmann A,Eimer S,Shaw AS,Kramer-Zucker A,Hirano-Kobayashi M,Abe T,Aizawa S,Grahammer F,Hartleben B,Dengjel J,Huber TB

Developmental vascular remodeling defects and postnatal kidney failure in mice lacking Gpr116 (Adgrf5) and Eltd1 (Adgrl4).
PloS one12(8)e0183166(2017)
Lu S,Liu S,Wietelmann A,Kojonazarov B,Atzberger A,Tang C,Schermuly RT,Gr?ne HJ,Offermanns S

An inducible mouse model of podocin-mutation-related nephrotic syndrome.
PloS one12(10)e0186574(2017)
Tabatabaeifar M,Wlodkowski T,Simic I,Denc H,Mollet G,Weber S,Moyers JJ,Br?hl B,Randles MJ,Lennon R,Antignac C,Schaefer F

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Nphs2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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