Ltc4s cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ltc4s
Entrez Gene ID	17001
Full Name	leukotriene C4 synthase
General protein information	Preferred Names leukotriene C4 synthase Names leukotriene C4 synthase LTC4 synthase leukotriene-C(4) synthase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.3
Summary	The protein encoded by this gene is an enzyme that catalyzes the synthesis of leukotriene C4 by combining leukotriene A4 with reduced glutathione. The encoded protein is found in the outer nuclear membrane and in the peripheral endoplasmic reticulum. Leukotrienes have been implicated as mediators of anaphylaxis and inflammatory conditions such as bronchial asthma in humans. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008521.2	NP_032547.1	leukotriene C4 synthase isoform 1
NM_001313968.1	NP_001300897.1	leukotriene C4 synthase isoform 2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	mgst2	NP_001106565.1
Pan troglodytes (chimpanzee)	LTC4S	XP_001153488.2
	LTC4S	XP_001101985.1
Canis lupus familiaris (dog)	LTC4S	XP_849032.2
Bos taurus (cattle)	LTC4S	NP_001039563.2
Gallus gallus (chicken)	LOC770619	XP_001233958.3
Mus musculus (house mouse)	Ltc4s	NP_032547.1
Homo sapiens (human)	LTC4S	NP_665874.1
Rattus norvegicus (Norway rat)	Ltc4s	NP_446091.2

Gene Ontology
Bibliography

Related articles in PubMed

Type 2 Cysteinyl Leukotriene Receptors Drive IL-33-Dependent Type 2 Immunopathology and Aspirin Sensitivity.
Liu T, Barrett NA, Kanaoka Y, Yoshimoto E, Garofalo D, Cirka H, Feng C, Boyce JA
Journal of immunology (Baltimore, Md. : 1950)200(3)915-927(2018 Feb)

Leukotrienes provide an NFAT-dependent signal that synergizes with IL-33 to activate ILC2s.
von Moltke J, O'Leary CE, Barrett NA, Kanaoka Y, Austen KF, Locksley RM
The Journal of experimental medicine214(1)27-37(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutational and kinetic data, together with molecular simulations, suggest that phosphorylation of Ser(36) inhibits the catalytic function of LTC4S by interference with the catalytic machinery.
Title: Phosphorylation of Leukotriene C4 Synthase at Serine 36 Impairs Catalytic Activity.

LTC4 synthase was selectively localized in the vasopressinergic magnocellular neurons of the hypothalamic paraventricular, supraoptic and suprachiasmatic nuclei and in the retrochiasmatic area.
Title: Highly selective localization of leukotriene C4 synthase in hypothalamic and extrahypothalamic vasopressin systems of mouse brain.

Expression of leukotriene C4 synthase mRNA in the choroid plexus.
Title: Expression of leukotriene C4 synthase mRNA by the choroid plexus in mouse brain suggests novel neurohormone functions of cysteinyl leukotrienes.

These results suggest that LTC(4) synthase, thought to be specific for the conjugation of GSH to LTA(4), can also recognize 5-oxoETE as an electrophilic substrate.
Title: Formation of murine macrophage-derived 5-oxo-7-glutathionyl-8,11,14-eicosatrienoic acid (FOG7) is catalyzed by leukotriene C4 synthase.

The following Ltc4s gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ltc4s cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu68158	NM_001313968.1 Latest version!	Mus musculus leukotriene C4 synthase (Ltc4s), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu02135	NM_008521.2 Latest version!	Mus musculus leukotriene C4 synthase (Ltc4s), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ltc4s ( NM_001313968.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300897.1 Mus musculus leukotriene C4 synthase (Ltc4s), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu68158
Clone ID Related Accession (Same CDS sequence)	NM_001313968.1
Accession Version	NM_001313968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	leukotriene C4 synthase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE848671.1, BC028760.1 and BC038518.1. On Sep 17, 2015 this sequence version replaced XM_011248777.1. Transcript Variant: This variant (2) uses an alternate in-frame exon in the 3' end compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BE630335.1, BC028760.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGACG AAGTGGCTCT TCTGGCTACC GTCACCCTCG TGGGAGTTCT GTTGCAAGCC 
TACTTCTCCC TACAGGTGAT CTCTGCACGA AGGGCTTTCC ACGTGTCGCC GCCGCTCACC 
TCTGGCCCTC CCGAGTTCGA GCGCGTCTTC CGAGCCCAGG TAAACTGCAG CGAGTACTTT 
CCGCTGTTCC TCGCCACACT CTGGGTCGCC GGCATCTTCT TCCACGAAGG AGCCGCAGCC 
CTGTGCGGAC TGTTCTACCT GTTCGCGCGC CTCCGCTATT TCCAGGGATA CGCGCGCTCA 
GCGCAACTCA GATGGCTCCA GATGCTCCTG CCGATGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300897.1
CDS	98..439
Translation	MKDEVALLATVTLVGVLLQAYFSLQVISARRAFHVSPPLTSGPPEFERVFRAQVNCSEYFPLFLATLWVAGIFFHEGAAALCGLFYLFARLRYFQGYARSAQLRWLQMLLPMA

Target ORF information:

RefSeq Version	NM_001313968.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus leukotriene C4 synthase (Ltc4s), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313968.1

ORF Insert Sequence:

ATGAAGGACG AAGTGGCTCT TCTGGCTACC GTCACCCTCG TGGGAGTTCT GTTGCAAGCC 
TACTTCTCCC TACAGGTGAT CTCTGCACGA AGGGCTTTCC ACGTGTCGCC GCCGCTCACC 
TCTGGCCCTC CCGAGTTCGA GCGCGTCTTC CGAGCCCAGG TAAACTGCAG CGAGTACTTT 
CCGCTGTTCC TCGCCACACT CTGGGTCGCC GGCATCTTCT TCCACGAAGG AGCCGCAGCC 
CTGTGCGGAC TGTTCTACCT GTTCGCGCGC CTCCGCTATT TCCAGGGATA CGCGCGCTCA 
GCGCAACTCA GATGGCTCCA GATGCTCCTG CCGATGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ltc4s ( NM_008521.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032547.1 Mus musculus leukotriene C4 synthase (Ltc4s), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02135
Clone ID Related Accession (Same CDS sequence)	NM_008521.2
Accession Version	NM_008521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	leukotriene C4 synthase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE848671.1, U27195.1 and BC038518.1. On Sep 17, 2015 this sequence version replaced NM_008521.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AI893906.1, AI385612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGACG AAGTGGCTCT TCTGGCTACC GTCACCCTCG TGGGAGTTCT GTTGCAAGCC 
TACTTCTCCC TACAGGTGAT CTCTGCACGA AGGGCTTTCC ACGTGTCGCC GCCGCTCACC 
TCTGGCCCTC CCGAGTTCGA GCGCGTCTTC CGAGCCCAGG TAAACTGCAG CGAGTACTTT 
CCGCTGTTCC TCGCCACACT CTGGGTCGCC GGCATCTTCT TCCACGAAGG AGCCGCAGCC 
CTGTGCGGAC TGTTCTACCT GTTCGCGCGC CTCCGCTATT TCCAGGGATA CGCGCGCTCA 
GCGCAACTCA GGCTGACTCC CCTATACGCG AGCGCGCGCG CACTCTGGCT GCTGGTGGCG 
ATGGCTGCAC TGGGCTTGCT AGTCCACTTC CTCCCCGGCA CGCTACGGAC TGCGCTCTTC 
AGATGGCTCC AGATGCTCCT GCCGATGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032547.1
CDS	98..550
Translation	MKDEVALLATVTLVGVLLQAYFSLQVISARRAFHVSPPLTSGPPEFERVFRAQVNCSEYFPLFLATLWVAGIFFHEGAAALCGLFYLFARLRYFQGYARSAQLRLTPLYASARALWLLVAMAALGLLVHFLPGTLRTALFRWLQMLLPMA

Target ORF information:

RefSeq Version	NM_008521.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus leukotriene C4 synthase (Ltc4s), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008521.2

ORF Insert Sequence:

ATGAAGGACG AAGTGGCTCT TCTGGCTACC GTCACCCTCG TGGGAGTTCT GTTGCAAGCC 
TACTTCTCCC TACAGGTGAT CTCTGCACGA AGGGCTTTCC ACGTGTCGCC GCCGCTCACC 
TCTGGCCCTC CCGAGTTCGA GCGCGTCTTC CGAGCCCAGG TAAACTGCAG CGAGTACTTT 
CCGCTGTTCC TCGCCACACT CTGGGTCGCC GGCATCTTCT TCCACGAAGG AGCCGCAGCC 
CTGTGCGGAC TGTTCTACCT GTTCGCGCGC CTCCGCTATT TCCAGGGATA CGCGCGCTCA 
GCGCAACTCA GGCTGACTCC CCTATACGCG AGCGCGCGCG CACTCTGGCT GCTGGTGGCG 
ATGGCTGCAC TGGGCTTGCT AGTCCACTTC CTCCCCGGCA CGCTACGGAC TGCGCTCTTC 
AGATGGCTCC AGATGCTCCT GCCGATGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Type 2 Cysteinyl Leukotriene Receptors Drive IL-33-Dependent Type 2 Immunopathology and Aspirin Sensitivity.
Journal of immunology (Baltimore, Md. : 1950)200(3)915-927(2018 Feb)
Liu T,Barrett NA,Kanaoka Y,Yoshimoto E,Garofalo D,Cirka H,Feng C,Boyce JA

Leukotrienes provide an NFAT-dependent signal that synergizes with IL-33 to activate ILC2s.
The Journal of experimental medicine214(1)27-37(2017 Jan)
von Moltke J,O'Leary CE,Barrett NA,Kanaoka Y,Austen KF,Locksley RM

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Ltc4s cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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