Lmo2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lmo2
Entrez Gene ID	16909
Full Name	LIM domain only 2
Synonyms	Rbtn-2,Rbtn2,Rhom-2,Ttg2
General protein information	Preferred Names LIM domain only 2 Names rhombotin-2 LIM domain only protein 2 LIM only 2 LMO-2 T-cell translocation protein 2 cysteine-rich protein TTG-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 E2\|2 54.46 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006498820.2	XP_006498883.1	rhombotin-2 isoform X1
XM_030247876.1	XP_030103736.1	rhombotin-2 isoform X1
XM_006498820.4	XP_006498883.1	rhombotin-2 isoform X1
XM_006498820.3	XP_006498883.1	rhombotin-2 isoform X1
NM_001142335.1	NP_001135807.1	rhombotin-2 isoform 2
NM_008505.4	NP_032531.2	rhombotin-2 isoform 1
NM_001142337.1	NP_001135809.1	rhombotin-2 isoform 3
NM_001142336.1	NP_001135808.1	rhombotin-2 isoform 3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LMO2	NP_989602.1
Mus musculus (house mouse)	Lmo2	NP_001135807.1
Danio rerio (zebrafish)	lmo2	NP_571186.1
	LMO2	XP_001115748.1
Canis lupus familiaris (dog)	LOC609006	XP_005631771.1
Rattus norvegicus (Norway rat)	Lmo2	NP_001032435.2
Xenopus tropicalis (tropical clawed frog)	lmo2	NP_001011238.1
Homo sapiens (human)	LMO2	NP_005565.2
Pan troglodytes (chimpanzee)	LMO2	XP_001145605.2

Gene Ontology
Bibliography

Related articles in PubMed

BRACHYURY directs histone acetylation to target loci during mesoderm development.
Beisaw A, Tsaytler P, Koch F, Schmitz SU, Melissari MT, Senft AD, Wittler L, Pennimpede T, Macura K, Herrmann BG, Grote P
EMBO reports19(1)118-134(2018 Jan)

LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage.
Stanulovic VS, Cauchy P, Assi SA, Hoogenkamp M
Nucleic acids research45(17)9874-9888(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genome-wide analysis indicated that LMO2 is required at the hemangioblast stage to position the TAL1/LMO2/LDB1 complex to regulatory elements that are important for the establishment of the hematopoietic developmental program.
Title: LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage.

DNM2 mutations cooperate with Lmo2 T-cell oncogenes by enhancing IL-7 signalling.
Title: Loss-of-function mutations of Dynamin 2 promote T-ALL by enhancing IL-7 signalling.

Hhex regulates Kit to promote radioresistance of self-renewing thymocytes in Lmo2-transgenic mice.
Title: Hhex regulates Kit to promote radioresistance of self-renewing thymocytes in Lmo2-transgenic mice.

HHEX is a direct transcriptional target of LMO2 consistent with its concordant gene expression.
Title: LIM domain only-2 (LMO2) induces T-cell leukemia by two distinct pathways.

Lyl1 is critical for all oncogenic functions of Lmo2, including upregulation of a stem cell-like gene signature, aberrant self-renewal of thymocytes, and subsequent generation of T-cell leukemia.
Title: Requirement for Lyl1 in a model of Lmo2-driven early T-cell precursor ALL.

The following Lmo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lmo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19724	NM_008505.4 Latest version!	Mus musculus LIM domain only 2 (Lmo2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu19324	NM_001142335.1 Latest version!	Mus musculus LIM domain only 2 (Lmo2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu19288	NM_001142337.1 Latest version!	Mus musculus LIM domain only 2 (Lmo2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu19288	NM_001142336.1 Latest version!	Mus musculus LIM domain only 2 (Lmo2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu19288	XM_030247876.1 Latest version!	Mus musculus LIM domain only 2 (Lmo2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu19288	XM_006498820.4 Latest version!	Mus musculus LIM domain only 2 (Lmo2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu19288	XM_006498820.2	Mus musculus LIM domain only 2 (Lmo2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu19288	XM_006498820.3	Mus musculus LIM domain only 2 (Lmo2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lmo2 ( NM_008505.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032531.2 Mus musculus LIM domain only 2 (Lmo2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19724
Clone ID Related Accession (Same CDS sequence)	NM_008505.4
Accession Version	NM_008505.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	rhombotin-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB864414.1, W76814.1, AK013416.1, BX523983.1 and AV361906.1. On Nov 27, 2008 this sequence version replaced NM_008505.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057880.1, BG293022.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGATTCGCT CTCTCTCTTT GGCGTTTGAA GGGAGCGCGG TGACTGTCCT TGAGCGTGGA 
GGGGCGAGCT CGCCAGCGGA GCGACCGAGC AAGAGGAGGC GCAGGAGCGG CGGCGCCCGA 
GCACCCGAGG GGGTCCGAGT CCAGGCAGCT AGCCAGCCAC GCGCCACAAA GGGAGCGCCC 
CCGCCGCCCG GCACGCCGCC CCCCTCCCCA ATGTCCTCGG CCATCGAAAG GAAGAGCCTG 
GACCCGTCTG AGGAACCCGT GGATGAGGTG CTGCAGATAC CCCCATCCCT GCTGACATGT 
GGTGGCTGCC AGCAGAACAT AGGGGACCGC TACTTCCTGA AAGCCATCGA CCAGTACTGG 
CATGAGGATT GCCTCAGCTG TGACCTCTGT GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC 
CTCTACTACA AGCTGGGACG GAAATTGTGC AGGAGAGACT ATCTCAGGCT TTTTGGTCAG 
GATGGTCTCT GTGCATCCTG TGACAAGCGG ATCCGTGCCT ATGAGATGAC GATGCGGGTG 
AAAGACAAAG TGTATCACCT GGAGTGTTTC AAATGCGCCG CCTGTCAGAA GCATTTCTGT 
GTAGGTGACA GATACCTTCT CATCAACTCC GACATAGTGT GTGAACAAGA CATCTACGAG 
TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032531.2
CDS	176..862
Translation	MIRSLSLAFEGSAVTVLERGGASSPAERPSKRRRRSGGARAPEGVRVQAASQPRATKGAPPPPGTPPPSPMSSAIERKSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCDKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGII

Target ORF information:

RefSeq Version	NM_008505.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus LIM domain only 2 (Lmo2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008505.4

ORF Insert Sequence:

ATGATTCGCT CTCTCTCTTT GGCGTTTGAA GGGAGCGCGG TGACTGTCCT TGAGCGTGGA 
GGGGCGAGCT CGCCAGCGGA GCGACCGAGC AAGAGGAGGC GCAGGAGCGG CGGCGCCCGA 
GCACCCGAGG GGGTCCGAGT CCAGGCAGCT AGCCAGCCAC GCGCCACAAA GGGAGCGCCC 
CCGCCGCCCG GCACGCCGCC CCCCTCCCCA ATGTCCTCGG CCATCGAAAG GAAGAGCCTG 
GACCCGTCTG AGGAACCCGT GGATGAGGTG CTGCAGATAC CCCCATCCCT GCTGACATGT 
GGTGGCTGCC AGCAGAACAT AGGGGACCGC TACTTCCTGA AAGCCATCGA CCAGTACTGG 
CATGAGGATT GCCTCAGCTG TGACCTCTGT GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC 
CTCTACTACA AGCTGGGACG GAAATTGTGC AGGAGAGACT ATCTCAGGCT TTTTGGTCAG 
GATGGTCTCT GTGCATCCTG TGACAAGCGG ATCCGTGCCT ATGAGATGAC GATGCGGGTG 
AAAGACAAAG TGTATCACCT GGAGTGTTTC AAATGCGCCG CCTGTCAGAA GCATTTCTGT 
GTAGGTGACA GATACCTTCT CATCAACTCC GACATAGTGT GTGAACAAGA CATCTACGAG 
TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmo2 ( NM_001142335.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001135807.1 Mus musculus LIM domain only 2 (Lmo2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19324
Clone ID Related Accession (Same CDS sequence)	NM_001142335.1
Accession Version	NM_001142335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	rhombotin-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB864414.1, CX228914.1, AK013416.1, BX523983.1 and AV361906.1. On Feb 28, 2009 this sequence version replaced XM_001479884.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. These differences allow translation initiation from an alternate start codon and an isoform (2) with a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK013416.1, CX225252.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGGGA GCGCGGTGAC TGTCCTTGAG CGTGGAGGGG CGAGCTCGCC AGCGGAGCGA 
CCGAGCAAGA GGAGGCGCAG GAGCGGCGGC GCCCGAGCAC CCGAGGGGGT CCGAGTCCAG 
GCAGCTAGCC AGCCACGCGC CACAAAGGGA GCGCCCCCGC CGCCCGGCAC GCCGCCCCCC 
TCCCCAATGT CCTCGGCCAT CGAAAGGAAG AGCCTGGACC CGTCTGAGGA ACCCGTGGAT 
GAGGTGCTGC AGATACCCCC ATCCCTGCTG ACATGTGGTG GCTGCCAGCA GAACATAGGG 
GACCGCTACT TCCTGAAAGC CATCGACCAG TACTGGCATG AGGATTGCCT CAGCTGTGAC 
CTCTGTGGGT GTCGGCTGGG AGAGGTGGGG AGGCGCCTCT ACTACAAGCT GGGACGGAAA 
TTGTGCAGGA GAGACTATCT CAGGCTTTTT GGTCAGGATG GTCTCTGTGC ATCCTGTGAC 
AAGCGGATCC GTGCCTATGA GATGACGATG CGGGTGAAAG ACAAAGTGTA TCACCTGGAG 
TGTTTCAAAT GCGCCGCCTG TCAGAAGCAT TTCTGTGTAG GTGACAGATA CCTTCTCATC 
AACTCCGACA TAGTGTGTGA ACAAGACATC TACGAGTGGA CCAAGATCAA TGGGATCATC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135807.1
CDS	73..735
Translation	MEGSAVTVLERGGASSPAERPSKRRRRSGGARAPEGVRVQAASQPRATKGAPPPPGTPPPSPMSSAIERKSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCDKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGII

Target ORF information:

RefSeq Version	NM_001142335.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LIM domain only 2 (Lmo2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142335.1

ORF Insert Sequence:

ATGGAAGGGA GCGCGGTGAC TGTCCTTGAG CGTGGAGGGG CGAGCTCGCC AGCGGAGCGA 
CCGAGCAAGA GGAGGCGCAG GAGCGGCGGC GCCCGAGCAC CCGAGGGGGT CCGAGTCCAG 
GCAGCTAGCC AGCCACGCGC CACAAAGGGA GCGCCCCCGC CGCCCGGCAC GCCGCCCCCC 
TCCCCAATGT CCTCGGCCAT CGAAAGGAAG AGCCTGGACC CGTCTGAGGA ACCCGTGGAT 
GAGGTGCTGC AGATACCCCC ATCCCTGCTG ACATGTGGTG GCTGCCAGCA GAACATAGGG 
GACCGCTACT TCCTGAAAGC CATCGACCAG TACTGGCATG AGGATTGCCT CAGCTGTGAC 
CTCTGTGGGT GTCGGCTGGG AGAGGTGGGG AGGCGCCTCT ACTACAAGCT GGGACGGAAA 
TTGTGCAGGA GAGACTATCT CAGGCTTTTT GGTCAGGATG GTCTCTGTGC ATCCTGTGAC 
AAGCGGATCC GTGCCTATGA GATGACGATG CGGGTGAAAG ACAAAGTGTA TCACCTGGAG 
TGTTTCAAAT GCGCCGCCTG TCAGAAGCAT TTCTGTGTAG GTGACAGATA CCTTCTCATC 
AACTCCGACA TAGTGTGTGA ACAAGACATC TACGAGTGGA CCAAGATCAA TGGGATCATC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmo2 ( NM_001142337.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001135809.1 Mus musculus LIM domain only 2 (Lmo2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu19288
Clone ID Related Accession (Same CDS sequence)	XM_006498820.2 , XM_030247876.1 , XM_006498820.4 , XM_006498820.3 , NM_001142337.1 , NM_001142336.1
Accession Version	NM_001142337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	rhombotin-2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from W76814.1, BB864414.1, AK013416.1, BX523983.1 and AV361906.1. Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an in-frame downstream ATG and an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3 and 4 encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DT903868.1, W64847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135809.1
CDS	269..745
Translation	MSSAIERKSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCDKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGII

Target ORF information:

RefSeq Version	NM_001142337.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LIM domain only 2 (Lmo2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142337.1

ORF Insert Sequence:

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmo2 ( NM_001142336.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001135808.1 Mus musculus LIM domain only 2 (Lmo2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu19288
Clone ID Related Accession (Same CDS sequence)	XM_006498820.2 , XM_030247876.1 , XM_006498820.4 , XM_006498820.3 , NM_001142337.1 , NM_001142336.1
Accession Version	NM_001142336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	rhombotin-2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX234489.1, CX226855.1, AK013416.1, BX523983.1 and AV361906.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an in-frame downstream ATG and an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3 and 4 encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CX228099.1, FO719916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135808.1
CDS	345..821
Translation	MSSAIERKSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCDKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGII

Target ORF information:

RefSeq Version	NM_001142336.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LIM domain only 2 (Lmo2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142336.1

ORF Insert Sequence:

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmo2 ( XM_006498820.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498883.1 Mus musculus LIM domain only 2 (Lmo2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19288
Clone ID Related Accession (Same CDS sequence)	XM_006498820.2 , XM_030247876.1 , XM_006498820.4 , XM_006498820.3 , NM_001142337.1 , NM_001142336.1
Accession Version	XM_006498820.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	rhombotin-2 isoform X1
Comment

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498883.1
Translation	MSSAIERKSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCDKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGII

Target ORF information:

RefSeq Version	XM_006498820.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus LIM domain only 2 (Lmo2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498820.2

ORF Insert Sequence:

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmo2 ( XM_030247876.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103736.1 Mus musculus LIM domain only 2 (Lmo2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19288
Clone ID Related Accession (Same CDS sequence)	XM_006498820.2 , XM_030247876.1 , XM_006498820.4 , XM_006498820.3 , NM_001142337.1 , NM_001142336.1
Accession Version	XM_030247876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rhombotin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103736.1
CDS	426..902
Translation	MSSAIERKSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCDKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGII

Target ORF information:

RefSeq Version	XM_030247876.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LIM domain only 2 (Lmo2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247876.1

ORF Insert Sequence:

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmo2 ( XM_006498820.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498883.1 Mus musculus LIM domain only 2 (Lmo2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu19288
Clone ID Related Accession (Same CDS sequence)	XM_006498820.2 , XM_030247876.1 , XM_006498820.4 , XM_006498820.3 , NM_001142337.1 , NM_001142336.1
Accession Version	XM_006498820.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rhombotin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498820.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498883.1
CDS	698..1174
Translation	MSSAIERKSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCDKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGII

Target ORF information:

RefSeq Version	XM_006498820.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus LIM domain only 2 (Lmo2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498820.4

ORF Insert Sequence:

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmo2 ( XM_006498820.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498883.1 Mus musculus LIM domain only 2 (Lmo2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19288
Clone ID Related Accession (Same CDS sequence)	XM_006498820.2 , XM_030247876.1 , XM_006498820.4 , XM_006498820.3 , NM_001142337.1 , NM_001142336.1
Accession Version	XM_006498820.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rhombotin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498820.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498883.1
CDS	308..784
Translation	MSSAIERKSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCDKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGII

Target ORF information:

RefSeq Version	XM_006498820.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus LIM domain only 2 (Lmo2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498820.3

ORF Insert Sequence:

ATGTCCTCGG CCATCGAAAG GAAGAGCCTG GACCCGTCTG AGGAACCCGT GGATGAGGTG 
CTGCAGATAC CCCCATCCCT GCTGACATGT GGTGGCTGCC AGCAGAACAT AGGGGACCGC 
TACTTCCTGA AAGCCATCGA CCAGTACTGG CATGAGGATT GCCTCAGCTG TGACCTCTGT 
GGGTGTCGGC TGGGAGAGGT GGGGAGGCGC CTCTACTACA AGCTGGGACG GAAATTGTGC 
AGGAGAGACT ATCTCAGGCT TTTTGGTCAG GATGGTCTCT GTGCATCCTG TGACAAGCGG 
ATCCGTGCCT ATGAGATGAC GATGCGGGTG AAAGACAAAG TGTATCACCT GGAGTGTTTC 
AAATGCGCCG CCTGTCAGAA GCATTTCTGT GTAGGTGACA GATACCTTCT CATCAACTCC 
GACATAGTGT GTGAACAAGA CATCTACGAG TGGACCAAGA TCAATGGGAT CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BRACHYURY directs histone acetylation to target loci during mesoderm development.
EMBO reports19(1)118-134(2018 Jan)
Beisaw A,Tsaytler P,Koch F,Schmitz SU,Melissari MT,Senft AD,Wittler L,Pennimpede T,Macura K,Herrmann BG,Grote P

LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage.
Nucleic acids research45(17)9874-9888(2017 Sep)
Stanulovic VS,Cauchy P,Assi SA,Hoogenkamp M

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Lmo2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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