Lcat cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lcat
Entrez Gene ID	16816
Full Name	lecithin cholesterol acyltransferase
Synonyms	AI046659,D8Wsu61e
General protein information	Preferred Names lecithin cholesterol acyltransferase Names phosphatidylcholine-sterol acyltransferase lecithin-cholesterol acyltransferase Lcat phospholipid-cholesterol acyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 D3\|8 53.06 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008490.2	NP_032516.2	phosphatidylcholine-sterol acyltransferase precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LCAT	XP_414027.4
Homo sapiens (human)	LCAT	NP_000220.1
Pan troglodytes (chimpanzee)	LCAT	XP_001166500.1
Mus musculus (house mouse)	Lcat	NP_032516.2
Canis lupus familiaris (dog)	LCAT	XP_851673.1
Danio rerio (zebrafish)	LOC793137	XP_001332828.3
Xenopus tropicalis (tropical clawed frog)	lcat	NP_001005715.1
Bos taurus (cattle)	LCAT	NP_001039534.1
Rattus norvegicus (Norway rat)	Lcat	NP_058720.2

Gene Ontology
Bibliography

Related articles in PubMed

Lipoprotein X Causes Renal Disease in LCAT Deficiency.
Ossoli A, Neufeld EB, Thacker SG, Vaisman B, Pryor M, Freeman LA, Brantner CA, Baranova I, Francone NO, Demosky SJ Jr, Vitali C, Locatelli M, Abbate M, Zoja C, Franceschini G, Calabresi L, Remaley AT
PloS one11(2)e0150083(2016)

Lecithin:Cholesterol Acyltransferase (LCAT) Deficiency Promotes Differentiation of Satellite Cells to Brown Adipocytes in a Cholesterol-dependent Manner.
Nesan D, Tavallaee G, Koh D, Bashiri A, Abdin R, Ng DS
The Journal of biological chemistry290(51)30514-29(2015 Dec)

Lack of LCAT reduces the LPS-neutralizing capacity of HDL and enhances LPS-induced inflammation in mice.
Petropoulou PI, Berb?e JF, Theodoropoulos V, Hatziri A, Stamou P, Karavia EA, Spyridonidis A, Karagiannides I, Kypreos KE
Biochimica et biophysica acta1852(10 Pt A)2106-15(2015 Oct)

Assignment of the mouse vesicular monoamine transporter genes, Slc18a1 and Slc18a2, to chromosomes 8 and 19 by linkage analysis.
Roghani A, Welch C, Xia Y, Liu Y, Peter D, Finn JP, Edwards RH, Lusis AJ
Mammalian genome : official journal of the International Mammalian Genome Society7(5)393-4(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

increased cholesterol esterification by LCAT is atheroprotective
Title: Increased plasma cholesterol esterification by LCAT reduces diet-induced atherosclerosis in SR-BI knockout mice.

Gene transfer of WT LCAT in LCAT(-/-) mice increased 11.8-fold the plasma cholesterol, whereas the LCAT[T147I] and LCAT[P274S] mutants caused a 5.2- and 2.9-fold increase, respectively.
Title: The Effect of Natural LCAT Mutations on the Biogenesis of HDL.

Taken together, these results suggest that apoE-containing discoidal HDLs do not require LCAT-dependent maturation to mediate efficient Abeta clearance
Title: LCAT deficiency does not impair amyloid metabolism in APP/PS1 mice.

DYRK1A overexpression decreases plasma lecithin:cholesterol acyltransferase activity and apolipoprotein A-I levels.
Title: DYRK1A overexpression decreases plasma lecithin:cholesterol acyltransferase activity and apolipoprotein A-I levels.

adrenal glucocorticoid function in LCAT knockout (KO) mice
Title: LCAT deficiency in mice is associated with a diminished adrenal glucocorticoid function.

The following Lcat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lcat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20458	NM_008490.2 Latest version!	Mus musculus lecithin cholesterol acyltransferase (Lcat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lcat ( NM_008490.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032516.2 Mus musculus lecithin cholesterol acyltransferase (Lcat), mRNA.

Price & Availability↑

CloneID	OMu20458
Clone ID Related Accession (Same CDS sequence)	NM_008490.2
Accession Version	NM_008490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	phosphatidylcholine-sterol acyltransferase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AI574112.1, AK149476.1 and AI046659.1. On Jul 2, 2009 this sequence version replaced NM_008490.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028861.1, AK149476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCTGC CTGGCTCCCC ATGGCAGCGG GTGCTGTTGC TGTTGGGGCT ACTGCTCCCT 
CCTGCCACCC CCTTCTGGCT CCTCAATGTG CTCTTCCCCC CGCACACCAC GCCCAAGGCT 
GAACTCAGTA ACCACACACG GCCTGTCATC CTCGTGCCTG GCTGCTTGGG GAATCGGCTA 
GAAGCCAAGC TGGATAAACC AGATGTGGTG AACTGGATGT GCTACCGTAA GACAGAGGAC 
TTCTTCACCA TCTGGCTGGA TTTCAACTTG TTTCTCCCCC TCGGGGTGGA CTGCTGGATT 
GATAATACCA GGATTGTCTA CAACCACAGC TCTGGGCGCG TGTCCAATGC CCCTGGTGTC 
CAGATCCGTG TCCCTGGCTT TGGCAAGACC GAATCTGTTG AGTACGTGGA TGACAACAAG 
CTAGCAGGCT ACCTGCACAC ACTGGTGCAG AATCTGGTTA ACAACGGATA TGTGCGGGAT 
GAGACAGTGC GGGCCGCACC CTATGACTGG CGTCTGGCAC CCCACCAGCA GGATGAATAC 
TACAAGAAGC TGGCTGGCCT GGTAGAGGAG ATGTATGCCG CTTATGGGAA GCCTGTCTTC 
CTCATTGGGC ATAGCCTTGG CTGTCTGCAT GTGCTCCACT TCTTACTGCG GCAGCCTCAG 
TCCTGGAAGG ACCACTTCAT TGATGGTTTT ATCTCTCTCG GGGCTCCGTG GGGTGGTTCC 
ATCAAGGCCA TGCGGATCCT GGCCTCAGGT GACAACCAGG GCATCCCCAT CCTGTCCAAC 
ATAAAGCTGA AAGAGGAGCA GCGCATAACC ACGACTTCCC CCTGGATGCT CCCAGCCCCT 
CACGTGTGGC CTGAAGACCA TGTGTTCATT TCCACACCAA ACTTCAACTA CACCGTCCAA 
GACTTTGAGC GTTTTTTCAC AGATCTGCAT TTTGAAGAAG GCTGGCACAT GTTTCTTCAG 
TCTCGTGACC TACTGGAGCG CCTCCCCGCA CCTGGTGTAG AAGTATATTG TCTCTACGGT 
GTGGGCAGAC CCACACCCCA CACCTACATC TATGACCACA ACTTCCCCTA CAAAGACCCC 
GTGGCTGCAC TCTATGAAGA TGGGGACGAC ACCGTAGCCA CCCGCAGCAC TGAGCTCTGT 
GGCCAGTGGC AGGGCCGCCA GTCGCAGCCC GTACATTTGC TGCCCATGAA CGAGACAGAT 
CACCTCAACA TGGTCTTCAG CAATAAGACA CTGGAGCATA TCAATGCCAT CCTACTGGGT 
GCCTACCGCA CTCCTAAGTC GCCAGCTGCC AGCCCAAGTC CCCCACCCCC TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032516.2
CDS	25..1341
Translation	MGLPGSPWQRVLLLLGLLLPPATPFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNRLEAKLDKPDVVNWMCYRKTEDFFTIWLDFNLFLPLGVDCWIDNTRIVYNHSSGRVSNAPGVQIRVPGFGKTESVEYVDDNKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLAPHQQDEYYKKLAGLVEEMYAAYGKPVFLIGHSLGCLHVLHFLLRQPQSWKDHFIDGFISLGAPWGGSIKAMRILASGDNQGIPILSNIKLKEEQRITTTSPWMLPAPHVWPEDHVFISTPNFNYTVQDFERFFTDLHFEEGWHMFLQSRDLLERLPAPGVEVYCLYGVGRPTPHTYIYDHNFPYKDPVAALYEDGDDTVATRSTELCGQWQGRQSQPVHLLPMNETDHLNMVFSNKTLEHINAILLGAYRTPKSPAASPSPPPPE

Target ORF information:

RefSeq Version	NM_008490.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lecithin cholesterol acyltransferase (Lcat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008490.2

ORF Insert Sequence:

ATGGGGCTGC CTGGCTCCCC ATGGCAGCGG GTGCTGTTGC TGTTGGGGCT ACTGCTCCCT 
CCTGCCACCC CCTTCTGGCT CCTCAATGTG CTCTTCCCCC CGCACACCAC GCCCAAGGCT 
GAACTCAGTA ACCACACACG GCCTGTCATC CTCGTGCCTG GCTGCTTGGG GAATCGGCTA 
GAAGCCAAGC TGGATAAACC AGATGTGGTG AACTGGATGT GCTACCGTAA GACAGAGGAC 
TTCTTCACCA TCTGGCTGGA TTTCAACTTG TTTCTCCCCC TCGGGGTGGA CTGCTGGATT 
GATAATACCA GGATTGTCTA CAACCACAGC TCTGGGCGCG TGTCCAATGC CCCTGGTGTC 
CAGATCCGTG TCCCTGGCTT TGGCAAGACC GAATCTGTTG AGTACGTGGA TGACAACAAG 
CTAGCAGGCT ACCTGCACAC ACTGGTGCAG AATCTGGTTA ACAACGGATA TGTGCGGGAT 
GAGACAGTGC GGGCCGCACC CTATGACTGG CGTCTGGCAC CCCACCAGCA GGATGAATAC 
TACAAGAAGC TGGCTGGCCT GGTAGAGGAG ATGTATGCCG CTTATGGGAA GCCTGTCTTC 
CTCATTGGGC ATAGCCTTGG CTGTCTGCAT GTGCTCCACT TCTTACTGCG GCAGCCTCAG 
TCCTGGAAGG ACCACTTCAT TGATGGTTTT ATCTCTCTCG GGGCTCCGTG GGGTGGTTCC 
ATCAAGGCCA TGCGGATCCT GGCCTCAGGT GACAACCAGG GCATCCCCAT CCTGTCCAAC 
ATAAAGCTGA AAGAGGAGCA GCGCATAACC ACGACTTCCC CCTGGATGCT CCCAGCCCCT 
CACGTGTGGC CTGAAGACCA TGTGTTCATT TCCACACCAA ACTTCAACTA CACCGTCCAA 
GACTTTGAGC GTTTTTTCAC AGATCTGCAT TTTGAAGAAG GCTGGCACAT GTTTCTTCAG 
TCTCGTGACC TACTGGAGCG CCTCCCCGCA CCTGGTGTAG AAGTATATTG TCTCTACGGT 
GTGGGCAGAC CCACACCCCA CACCTACATC TATGACCACA ACTTCCCCTA CAAAGACCCC 
GTGGCTGCAC TCTATGAAGA TGGGGACGAC ACCGTAGCCA CCCGCAGCAC TGAGCTCTGT 
GGCCAGTGGC AGGGCCGCCA GTCGCAGCCC GTACATTTGC TGCCCATGAA CGAGACAGAT 
CACCTCAACA TGGTCTTCAG CAATAAGACA CTGGAGCATA TCAATGCCAT CCTACTGGGT 
GCCTACCGCA CTCCTAAGTC GCCAGCTGCC AGCCCAAGTC CCCCACCCCC TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lipoprotein X Causes Renal Disease in LCAT Deficiency.
PloS one11(2)e0150083(2016)
Ossoli A,Neufeld EB,Thacker SG,Vaisman B,Pryor M,Freeman LA,Brantner CA,Baranova I,Francone NO,Demosky SJ Jr,Vitali C,Locatelli M,Abbate M,Zoja C,Franceschini G,Calabresi L,Remaley AT

Lecithin:Cholesterol Acyltransferase (LCAT) Deficiency Promotes Differentiation of Satellite Cells to Brown Adipocytes in a Cholesterol-dependent Manner.
The Journal of biological chemistry290(51)30514-29(2015 Dec)
Nesan D,Tavallaee G,Koh D,Bashiri A,Abdin R,Ng DS

Lack of LCAT reduces the LPS-neutralizing capacity of HDL and enhances LPS-induced inflammation in mice.
Biochimica et biophysica acta1852(10 Pt A)2106-15(2015 Oct)
Petropoulou PI,Berb?e JF,Theodoropoulos V,Hatziri A,Stamou P,Karavia EA,Spyridonidis A,Karagiannides I,Kypreos KE

Assignment of the mouse vesicular monoamine transporter genes, Slc18a1 and Slc18a2, to chromosomes 8 and 19 by linkage analysis.
Mammalian genome : official journal of the International Mammalian Genome Society7(5)393-4(1996 May)
Roghani A,Welch C,Xia Y,Liu Y,Peter D,Finn JP,Edwards RH,Lusis AJ

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Lcat cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報