Large1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Large1
Entrez Gene ID	16795
Full Name	LARGE xylosyl- and glucuronyltransferase 1
Synonyms	BPFD#36,Gyltl1a,Large,Mbp-1,Mbp1,enr,fg,froggy,myd
General protein information	Preferred Names LARGE xylosyl- and glucuronyltransferase 1 Names LARGE xylosyl- and glucuronyltransferase 1 glycosyltransferase-like protein LARGE1 acetylglucosaminyltransferase-like 1A like-glycosyltransferase myodystrophy
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 B3.3\|8 35.08 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011248317.1	XP_011246619.1	glycosyltransferase-like protein LARGE1 isoform X1
NM_010687.2	NP_034817.1	LARGE xylosyl- and glucuronyltransferase 1
XM_011248318.2	XP_011246620.1	glycosyltransferase-like protein LARGE1 isoform X1
NM_010687.1	NP_034817.1	glycosyltransferase-like protein LARGE1
NM_001317391.1	NP_001304320.1	LARGE xylosyl- and glucuronyltransferase 1
XM_030243312.1	XP_030099172.1	LARGE xylosyl- and glucuronyltransferase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LARGE	NP_004728.1
Canis lupus familiaris (dog)	LARGE	XP_005625982.1
	LARGE	XP_001113031.1
Pan troglodytes (chimpanzee)	LARGE	XP_001154608.1
Gallus gallus (chicken)	LARGE	NP_001004383.1
Mus musculus (house mouse)	Large	NP_034817.1
Danio rerio (zebrafish)	large	NP_001004537.1
Bos taurus (cattle)	LARGE	NP_001192517.1
Xenopus tropicalis (tropical clawed frog)	LOC100498555	XP_002943094.2

Gene Ontology
Bibliography

Related articles in PubMed

Degree of Cajal-Retzius Cell Mislocalization Correlates with the Severity of Structural Brain Defects in Mouse Models of Dystroglycanopathy.
Booler HS, Williams JL, Hopkinson M, Brown SC
Brain pathology (Zurich, Switzerland)26(4)465-78(2016 Jul)

Transgenic Rescue of the LARGEmyd Mouse: A LARGE Therapeutic Window?
Hildyard JC, Lacey E, Booler H, Hopkinson M, Wells DJ, Brown SC
PloS one11(7)e0159853(2016)

Restoration of Functional Glycosylation of ?-Dystroglycan in FKRP Mutant Mice Is Associated with Muscle Regeneration.
Awano H, Blaeser A, Keramaris E, Xu L, Tucker J, Wu B, Lu P, Lu QL
The American journal of pathology185(7)2025-37(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data suggest LARGE overexpression may only be deleterious under a forced regenerative context, such as that resulting from a reduction in FKRP: in the absence of such a defect we show that systemic expression of LARGE can indeed act therapeutically, and that even dramatic LARGE overexpression is well-tolerated in heart and skeletal muscle
Title: Transgenic Rescue of the LARGEmyd Mouse: A LARGE Therapeutic Window?

MBP1 inhibits Ab fibril formation in vitro and demonstrate the ability of MBP1 to reduce Ab pathology and improve behavioral performance.
Title: N-terminal region of myelin basic protein reduces fibrillar amyloid-? deposition in Tg-5xFAD mice.

Endogenous glucuronyltransferase activity of LARGE or LARGE2 required for functional modification of alpha-dystroglycan in cells and tissues.
Title: Endogenous glucuronyltransferase activity of LARGE or LARGE2 required for functional modification of ?-dystroglycan in cells and tissues.

A glucuronic acid beta1,4-xylose disaccharide synthesized by B4GAT1 acts as an acceptor primer that can be elongated by LARGE with the ligand-binding heteropolysaccharide.
Title: The glucuronyltransferase B4GAT1 is required for initiation of LARGE-mediated ?-dystroglycan functional glycosylation.

The overexpression of LARGE aggravates muscular dystrophy by suppressing the muscle regeneration and this adverse effect is mediated via reduced expression of IGF-1.
Title: Overexpression of LARGE suppresses muscle regeneration via down-regulation of insulin-like growth factor 1 and aggravates muscular dystrophy in mice.

The following Large1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Large1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68120	XM_011248318.2 Latest version!	Mus musculus like-glycosyltransferase (Large), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu18580	NM_001317391.1 Latest version!	Mus musculus LARGE xylosyl- and glucuronyltransferase 1 (Large1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu18580	NM_010687.2 Latest version!	Mus musculus LARGE xylosyl- and glucuronyltransferase 1 (Large1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu18580	XM_011248317.1 Latest version!	Mus musculus like-glycosyltransferase (Large), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu89752	XM_030243312.1 Latest version!	Mus musculus LARGE xylosyl- and glucuronyltransferase 1 (Large1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu18580	NM_010687.1	Mus musculus like-glycosyltransferase (Large), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Large1 ( XM_011248318.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246620.1 Mus musculus like-glycosyltransferase (Large), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68120
Clone ID Related Accession (Same CDS sequence)	XM_011248318.2
Accession Version	XM_011248318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glycosyltransferase-like protein LARGE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGAA TCTGCAGAGG GAGAAGGAAA TTCCTGGCTG CCTCTTTGAC TCTACTCTGC 
ATACCAGCCA TCACCTGGAT TTACTTGTTT GCTGGGAGCT TTGAAGATGG GAAACCTGTG 
TCTCTGTCTC CCCTGGAGTC CCAAGCCCAC AGTCCCCGGT ACACAGCCTC CAGCCAACGG 
GAGCGGGAGA GCCTGGAAGT GCGTGTTCGA GAAGTTGAGG AGGAAAACCG TGCTCTGCGC 
AGGCAGCTCA GCCTGGCCCA GGGTCAGAGC CCTGCTCACC ACCGTGGGAA CCACTCCAAG 
ACCTACTCCA TGGAGGAGGG GACAGGGGAC AGTGAGAATC TGCGAGCTGG CATTGTGGCA 
GGTAACAGCT CTGAGTGTGG GCAGCAGCCA GCTGTGGAAA AGTGTGAGAC CATCCATGTG 
GCCATCGTCT GTGCCGGATA CAATGCCAGC AGGGACGTTG TCACCCTGGT GAAATCTGTC 
CTCTTTCATA GGCGGAACCC TCTGCACTTC CACCTCATTG CTGATTCTAT TGCTGAGCAG 
ATCCTGGCTA CACTCTTCCA GACCTGGATG GTACCTGCAG TGCGAGTAGA CTTCTATAAT 
GCTGATGAGC TCAAGTCTGA AGTGTCCTGG ATTCCCAACA AACATTACTC TGGAATTTAT 
GGCCTGATGA AGCTGGTTCT GACCAAAACT CTGCCTGCCA ACCTGGAGAG AGTCATTGTC 
CTAGACACCG ACATCACCTT TGCAACAGAC ATTGCAGAGC TGTGGGCCGT GTTCCATAAG 
TTCAAAGGGC AACAGGTCCT GGGCTTGGTA GAGAACCAGA GTGACTGGTA TCTTGGAAAT 
CTCTGGAAAA ACCACCGTCC ATGGCCAGCT CTTGGAAGGG GCTATAACAC AGGAGTCATA 
CTACTGCTTC TGGACAAGCT TCGGAAGATG AAATGGGAGC AGATGTGGAG ACTGACAGCA 
GAGAGGGAAC TCATGGGCAT GTTGTCTACA TCCTTGGCTG ACCAGGATAT TTTCAATGCT 
GTTATCAAAC AAAACCCCTT TCTGGTGTAT CAGCTTCCCT GCTTCTGGAA TGTGCAGCTG 
TCAGACCACA CCCGCTCTGA GCAGTGCTAC AGAGATGTGT CTGATTTAAA GGTCATCCAC 
TGGAACTCCC CCAAGAAGCT CCGTGTGAAG AACAAACATG TGGAATTCTT CCGCAACCTC 
TACCTGACCT TCTTGGAGTA TGATGGCAAC CTCCTGCGGC GGGAGCTGTT TGGCTGCCCC 
AGTGAGACTG ATGTCAACAA TGAAAATCTT CAGAAGCAGC TGTCTGAGCT GGATGAGGAT 
GACTTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACAGAACCCA CCTGTACTTC 
CTGCACTACG AGTTTGAGCC TTCTGCTGAC AACACAGACG TTACCCTTGT TGCTCAGCTC 
TCCATGGACA GACTACAGAT GCTGGAGGCC ATCTGTAAAC ACTGGGAGGG ACCCATCAGC 
CTGGCCCTCT ACCTGTCAGA TGCAGAAGCC CAGCAGTTCC TCCGCTATGC CCAGGGATCC 
GAGGTGCTCA TGAGCCGGCA GAACGTGGGC TACCACATCG TGTACAAGGA GGGTCAGTTC 
TATCCTGTCA ATCTTCTGCG AAACGTGGCT ATGAAGCACA TCAGCACACC CTACATGTTC 
TTATCTGACA TTGACTTCCT GCCTATGTAT GGGCTCTATG AGTACCTCAG GAAATCTGTC 
ATCCAGCTTG ACCTTGCCAA TACCAAGAAA GCAATGATTG TCCCAGCATT TGAAACGCTG 
CGCTACAGAC TCTCTTTCCC CAAGTCAAAA GCAGAGCTGC TGTCAATGTT GGACATGGGG 
ACCCTATTCA CATTCAGGTT TGGGAAAATT CAGCTTGCAT CCCTTCACTC AATGGCTTTT 
TTCTACATGT CCCAGGTATC ACGTATGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246620.1
CDS	1195..3147
Translation	MLGICRGRRKFLAASLTLLCIPAITWIYLFAGSFEDGKPVSLSPLESQAHSPRYTASSQRERESLEVRVREVEEENRALRRQLSLAQGQSPAHHRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPAVEKCETIHVAIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSWIPNKHYSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGLVENQSDWYLGNLWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAERELMGMLSTSLADQDIFNAVIKQNPFLVYQLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRRELFGCPSETDVNNENLQKQLSELDEDDLCYEFRRERFTVHRTHLYFLHYEFEPSADNTDVTLVAQLSMDRLQMLEAICKHWEGPISLALYLSDAEAQQFLRYAQGSEVLMSRQNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMFLSDIDFLPMYGLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFTFRFGKIQLASLHSMAFFYMSQVSRMD

Target ORF information:

RefSeq Version	XM_011248318.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus like-glycosyltransferase (Large), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248318.2

ORF Insert Sequence:

ATGCTGGGAA TCTGCAGAGG GAGAAGGAAA TTCCTGGCTG CCTCTTTGAC TCTACTCTGC 
ATACCAGCCA TCACCTGGAT TTACTTGTTT GCTGGGAGCT TTGAAGATGG GAAACCTGTG 
TCTCTGTCTC CCCTGGAGTC CCAAGCCCAC AGTCCCCGGT ACACAGCCTC CAGCCAACGG 
GAGCGGGAGA GCCTGGAAGT GCGTGTTCGA GAAGTTGAGG AGGAAAACCG TGCTCTGCGC 
AGGCAGCTCA GCCTGGCCCA GGGTCAGAGC CCTGCTCACC ACCGTGGGAA CCACTCCAAG 
ACCTACTCCA TGGAGGAGGG GACAGGGGAC AGTGAGAATC TGCGAGCTGG CATTGTGGCA 
GGTAACAGCT CTGAGTGTGG GCAGCAGCCA GCTGTGGAAA AGTGTGAGAC CATCCATGTG 
GCCATCGTCT GTGCCGGATA CAATGCCAGC AGGGACGTTG TCACCCTGGT GAAATCTGTC 
CTCTTTCATA GGCGGAACCC TCTGCACTTC CACCTCATTG CTGATTCTAT TGCTGAGCAG 
ATCCTGGCTA CACTCTTCCA GACCTGGATG GTACCTGCAG TGCGAGTAGA CTTCTATAAT 
GCTGATGAGC TCAAGTCTGA AGTGTCCTGG ATTCCCAACA AACATTACTC TGGAATTTAT 
GGCCTGATGA AGCTGGTTCT GACCAAAACT CTGCCTGCCA ACCTGGAGAG AGTCATTGTC 
CTAGACACCG ACATCACCTT TGCAACAGAC ATTGCAGAGC TGTGGGCCGT GTTCCATAAG 
TTCAAAGGGC AACAGGTCCT GGGCTTGGTA GAGAACCAGA GTGACTGGTA TCTTGGAAAT 
CTCTGGAAAA ACCACCGTCC ATGGCCAGCT CTTGGAAGGG GCTATAACAC AGGAGTCATA 
CTACTGCTTC TGGACAAGCT TCGGAAGATG AAATGGGAGC AGATGTGGAG ACTGACAGCA 
GAGAGGGAAC TCATGGGCAT GTTGTCTACA TCCTTGGCTG ACCAGGATAT TTTCAATGCT 
GTTATCAAAC AAAACCCCTT TCTGGTGTAT CAGCTTCCCT GCTTCTGGAA TGTGCAGCTG 
TCAGACCACA CCCGCTCTGA GCAGTGCTAC AGAGATGTGT CTGATTTAAA GGTCATCCAC 
TGGAACTCCC CCAAGAAGCT CCGTGTGAAG AACAAACATG TGGAATTCTT CCGCAACCTC 
TACCTGACCT TCTTGGAGTA TGATGGCAAC CTCCTGCGGC GGGAGCTGTT TGGCTGCCCC 
AGTGAGACTG ATGTCAACAA TGAAAATCTT CAGAAGCAGC TGTCTGAGCT GGATGAGGAT 
GACTTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACAGAACCCA CCTGTACTTC 
CTGCACTACG AGTTTGAGCC TTCTGCTGAC AACACAGACG TTACCCTTGT TGCTCAGCTC 
TCCATGGACA GACTACAGAT GCTGGAGGCC ATCTGTAAAC ACTGGGAGGG ACCCATCAGC 
CTGGCCCTCT ACCTGTCAGA TGCAGAAGCC CAGCAGTTCC TCCGCTATGC CCAGGGATCC 
GAGGTGCTCA TGAGCCGGCA GAACGTGGGC TACCACATCG TGTACAAGGA GGGTCAGTTC 
TATCCTGTCA ATCTTCTGCG AAACGTGGCT ATGAAGCACA TCAGCACACC CTACATGTTC 
TTATCTGACA TTGACTTCCT GCCTATGTAT GGGCTCTATG AGTACCTCAG GAAATCTGTC 
ATCCAGCTTG ACCTTGCCAA TACCAAGAAA GCAATGATTG TCCCAGCATT TGAAACGCTG 
CGCTACAGAC TCTCTTTCCC CAAGTCAAAA GCAGAGCTGC TGTCAATGTT GGACATGGGG 
ACCCTATTCA CATTCAGGTT TGGGAAAATT CAGCTTGCAT CCCTTCACTC AATGGCTTTT 
TTCTACATGT CCCAGGTATC ACGTATGGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Large1 ( NM_010687.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034817.1 Mus musculus LARGE xylosyl- and glucuronyltransferase 1 (Large1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18580
Clone ID Related Accession (Same CDS sequence)	XM_011248317.1 , NM_010687.2 , NM_010687.1 , NM_001317391.1
Accession Version	NM_010687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	LARGE xylosyl- and glucuronyltransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157365.5, BQ442282.1, CN699283.1, AK133940.1, AJ006278.1 and AW490661.1. On Dec 3, 2015 this sequence version replaced NM_010687.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK133940.1, BC100399.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGGAA TCTGCAGAGG GAGAAGGAAA TTCCTGGCTG CCTCTTTGAC TCTACTCTGC 
ATACCAGCCA TCACCTGGAT TTACTTGTTT GCTGGGAGCT TTGAAGATGG GAAACCTGTG 
TCTCTGTCTC CCCTGGAGTC CCAAGCCCAC AGTCCCCGGT ACACAGCCTC CAGCCAACGG 
GAGCGGGAGA GCCTGGAAGT GCGTGTTCGA GAAGTTGAGG AGGAAAACCG TGCTCTGCGC 
AGGCAGCTCA GCCTGGCCCA GGGTCAGAGC CCTGCTCACC ACCGTGGGAA CCACTCCAAG 
ACCTACTCCA TGGAGGAGGG GACAGGGGAC AGTGAGAATC TGCGAGCTGG CATTGTGGCA 
GGTAACAGCT CTGAGTGTGG GCAGCAGCCA GCTGTGGAAA AGTGTGAGAC CATCCATGTG 
GCCATCGTCT GTGCCGGATA CAATGCCAGC AGGGACGTTG TCACCCTGGT GAAATCTGTC 
CTCTTTCATA GGCGGAACCC TCTGCACTTC CACCTCATTG CTGATTCTAT TGCTGAGCAG 
ATCCTGGCTA CACTCTTCCA GACCTGGATG GTACCTGCAG TGCGAGTAGA CTTCTATAAT 
GCTGATGAGC TCAAGTCTGA AGTGTCCTGG ATTCCCAACA AACATTACTC TGGAATTTAT 
GGCCTGATGA AGCTGGTTCT GACCAAAACT CTGCCTGCCA ACCTGGAGAG AGTCATTGTC 
CTAGACACCG ACATCACCTT TGCAACAGAC ATTGCAGAGC TGTGGGCCGT GTTCCATAAG 
TTCAAAGGGC AACAGGTCCT GGGCTTGGTA GAGAACCAGA GTGACTGGTA TCTTGGAAAT 
CTCTGGAAAA ACCACCGTCC ATGGCCAGCT CTTGGAAGGG GCTATAACAC AGGAGTCATA 
CTACTGCTTC TGGACAAGCT TCGGAAGATG AAATGGGAGC AGATGTGGAG ACTGACAGCA 
GAGAGGGAAC TCATGGGCAT GTTGTCTACA TCCTTGGCTG ACCAGGATAT TTTCAATGCT 
GTTATCAAAC AAAACCCCTT TCTGGTGTAT CAGCTTCCCT GCTTCTGGAA TGTGCAGCTG 
TCAGACCACA CCCGCTCTGA GCAGTGCTAC AGAGATGTGT CTGATTTAAA GGTCATCCAC 
TGGAACTCCC CCAAGAAGCT CCGTGTGAAG AACAAACATG TGGAATTCTT CCGCAACCTC 
TACCTGACCT TCTTGGAGTA TGATGGCAAC CTCCTGCGGC GGGAGCTGTT TGGCTGCCCC 
AGTGAGACTG ATGTCAACAA TGAAAATCTT CAGAAGCAGC TGTCTGAGCT GGATGAGGAT 
GACTTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACAGAACCCA CCTGTACTTC 
CTGCACTACG AGTTTGAGCC TTCTGCTGAC AACACAGACG TTACCCTTGT TGCTCAGCTC 
TCCATGGACA GACTACAGAT GCTGGAGGCC ATCTGTAAAC ACTGGGAGGG ACCCATCAGC 
CTGGCCCTCT ACCTGTCAGA TGCAGAAGCC CAGCAGTTCC TCCGCTATGC CCAGGGATCC 
GAGGTGCTCA TGAGCCGGCA GAACGTGGGC TACCACATCG TGTACAAGGA GGGTCAGTTC 
TATCCTGTCA ATCTTCTGCG AAACGTGGCT ATGAAGCACA TCAGCACACC CTACATGTTC 
TTATCTGACA TTGACTTCCT GCCTATGTAT GGGCTCTATG AGTACCTCAG GAAATCTGTC 
ATCCAGCTTG ACCTTGCCAA TACCAAGAAA GCAATGATTG TCCCAGCATT TGAAACGCTG 
CGCTACAGAC TCTCTTTCCC CAAGTCAAAA GCAGAGCTGC TGTCAATGTT GGACATGGGG 
ACCCTATTCA CATTCAGGTA TCACGTATGG ACTAAAGGCC ATGCACCCAC AAACTTTGCC 
AAGTGGCGGA CTGCCACCAC ACCTTACCAG GTTGAGTGGG AGGCTGATTT TGAGCCATAT 
GTTGTTGTGA GACGAGACTG CCCTGAGTAT GATCGGAGAT TTGTGGGCTT TGGCTGGAAC 
AAAGTGGCTC ACATCATGGA ATTGGATGCA CAGGAGTATG AGTTCACCGT GTTGCCCAAT 
GCCTACATGA TCCACATGCC CCATGCCCCT AGTTTCGACA TCACTAAGTT CCGTTCCAAC 
AAGCAATACC GCATCTGTCT CAAAACCCTG AAGGAAGAGT TTCAACAAGA CATGTCCCGA 
CGATATGGCT TTGCTGCCTT GAAATATCTC ACAGCTGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034817.1
CDS	1158..3428
Translation	MLGICRGRRKFLAASLTLLCIPAITWIYLFAGSFEDGKPVSLSPLESQAHSPRYTASSQRERESLEVRVREVEEENRALRRQLSLAQGQSPAHHRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPAVEKCETIHVAIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSWIPNKHYSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGLVENQSDWYLGNLWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAERELMGMLSTSLADQDIFNAVIKQNPFLVYQLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRRELFGCPSETDVNNENLQKQLSELDEDDLCYEFRRERFTVHRTHLYFLHYEFEPSADNTDVTLVAQLSMDRLQMLEAICKHWEGPISLALYLSDAEAQQFLRYAQGSEVLMSRQNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMFLSDIDFLPMYGLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFTFRYHVWTKGHAPTNFAKWRTATTPYQVEWEADFEPYVVVRRDCPEYDRRFVGFGWNKVAHIMELDAQEYEFTVLPNAYMIHMPHAPSFDITKFRSNKQYRICLKTLKEEFQQDMSRRYGFAALKYLTAENNS

Target ORF information:

RefSeq Version	NM_010687.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus LARGE xylosyl- and glucuronyltransferase 1 (Large1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010687.2

ORF Insert Sequence:

ATGCTGGGAA TCTGCAGAGG GAGAAGGAAA TTCCTGGCTG CCTCTTTGAC TCTACTCTGC 
ATACCAGCCA TCACCTGGAT TTACTTGTTT GCTGGGAGCT TTGAAGATGG GAAACCTGTG 
TCTCTGTCTC CCCTGGAGTC CCAAGCCCAC AGTCCCCGGT ACACAGCCTC CAGCCAACGG 
GAGCGGGAGA GCCTGGAAGT GCGTGTTCGA GAAGTTGAGG AGGAAAACCG TGCTCTGCGC 
AGGCAGCTCA GCCTGGCCCA GGGTCAGAGC CCTGCTCACC ACCGTGGGAA CCACTCCAAG 
ACCTACTCCA TGGAGGAGGG GACAGGGGAC AGTGAGAATC TGCGAGCTGG CATTGTGGCA 
GGTAACAGCT CTGAGTGTGG GCAGCAGCCA GCTGTGGAAA AGTGTGAGAC CATCCATGTG 
GCCATCGTCT GTGCCGGATA CAATGCCAGC AGGGACGTTG TCACCCTGGT GAAATCTGTC 
CTCTTTCATA GGCGGAACCC TCTGCACTTC CACCTCATTG CTGATTCTAT TGCTGAGCAG 
ATCCTGGCTA CACTCTTCCA GACCTGGATG GTACCTGCAG TGCGAGTAGA CTTCTATAAT 
GCTGATGAGC TCAAGTCTGA AGTGTCCTGG ATTCCCAACA AACATTACTC TGGAATTTAT 
GGCCTGATGA AGCTGGTTCT GACCAAAACT CTGCCTGCCA ACCTGGAGAG AGTCATTGTC 
CTAGACACCG ACATCACCTT TGCAACAGAC ATTGCAGAGC TGTGGGCCGT GTTCCATAAG 
TTCAAAGGGC AACAGGTCCT GGGCTTGGTA GAGAACCAGA GTGACTGGTA TCTTGGAAAT 
CTCTGGAAAA ACCACCGTCC ATGGCCAGCT CTTGGAAGGG GCTATAACAC AGGAGTCATA 
CTACTGCTTC TGGACAAGCT TCGGAAGATG AAATGGGAGC AGATGTGGAG ACTGACAGCA 
GAGAGGGAAC TCATGGGCAT GTTGTCTACA TCCTTGGCTG ACCAGGATAT TTTCAATGCT 
GTTATCAAAC AAAACCCCTT TCTGGTGTAT CAGCTTCCCT GCTTCTGGAA TGTGCAGCTG 
TCAGACCACA CCCGCTCTGA GCAGTGCTAC AGAGATGTGT CTGATTTAAA GGTCATCCAC 
TGGAACTCCC CCAAGAAGCT CCGTGTGAAG AACAAACATG TGGAATTCTT CCGCAACCTC 
TACCTGACCT TCTTGGAGTA TGATGGCAAC CTCCTGCGGC GGGAGCTGTT TGGCTGCCCC 
AGTGAGACTG ATGTCAACAA TGAAAATCTT CAGAAGCAGC TGTCTGAGCT GGATGAGGAT 
GACTTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACAGAACCCA CCTGTACTTC 
CTGCACTACG AGTTTGAGCC TTCTGCTGAC AACACAGACG TTACCCTTGT TGCTCAGCTC 
TCCATGGACA GACTACAGAT GCTGGAGGCC ATCTGTAAAC ACTGGGAGGG ACCCATCAGC 
CTGGCCCTCT ACCTGTCAGA TGCAGAAGCC CAGCAGTTCC TCCGCTATGC CCAGGGATCC 
GAGGTGCTCA TGAGCCGGCA GAACGTGGGC TACCACATCG TGTACAAGGA GGGTCAGTTC 
TATCCTGTCA ATCTTCTGCG AAACGTGGCT ATGAAGCACA TCAGCACACC CTACATGTTC 
TTATCTGACA TTGACTTCCT GCCTATGTAT GGGCTCTATG AGTACCTCAG GAAATCTGTC 
ATCCAGCTTG ACCTTGCCAA TACCAAGAAA GCAATGATTG TCCCAGCATT TGAAACGCTG 
CGCTACAGAC TCTCTTTCCC CAAGTCAAAA GCAGAGCTGC TGTCAATGTT GGACATGGGG 
ACCCTATTCA CATTCAGGTA TCACGTATGG ACTAAAGGCC ATGCACCCAC AAACTTTGCC 
AAGTGGCGGA CTGCCACCAC ACCTTACCAG GTTGAGTGGG AGGCTGATTT TGAGCCATAT 
GTTGTTGTGA GACGAGACTG CCCTGAGTAT GATCGGAGAT TTGTGGGCTT TGGCTGGAAC 
AAAGTGGCTC ACATCATGGA ATTGGATGCA CAGGAGTATG AGTTCACCGT GTTGCCCAAT 
GCCTACATGA TCCACATGCC CCATGCCCCT AGTTTCGACA TCACTAAGTT CCGTTCCAAC 
AAGCAATACC GCATCTGTCT CAAAACCCTG AAGGAAGAGT TTCAACAAGA CATGTCCCGA 
CGATATGGCT TTGCTGCCTT GAAATATCTC ACAGCTGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Large1 ( NM_010687.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034817.1 Mus musculus like-glycosyltransferase (Large), mRNA.

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CloneID	OMu18580
Clone ID Related Accession (Same CDS sequence)	XM_011248317.1 , NM_010687.2 , NM_010687.1 , NM_001317391.1
Accession Version	NM_010687.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	glycosyltransferase-like protein LARGE1
Comment

ATGCTGGGAA TCTGCAGAGG GAGAAGGAAA TTCCTGGCTG CCTCTTTGAC TCTACTCTGC 
ATACCAGCCA TCACCTGGAT TTACTTGTTT GCTGGGAGCT TTGAAGATGG GAAACCTGTG 
TCTCTGTCTC CCCTGGAGTC CCAAGCCCAC AGTCCCCGGT ACACAGCCTC CAGCCAACGG 
GAGCGGGAGA GCCTGGAAGT GCGTGTTCGA GAAGTTGAGG AGGAAAACCG TGCTCTGCGC 
AGGCAGCTCA GCCTGGCCCA GGGTCAGAGC CCTGCTCACC ACCGTGGGAA CCACTCCAAG 
ACCTACTCCA TGGAGGAGGG GACAGGGGAC AGTGAGAATC TGCGAGCTGG CATTGTGGCA 
GGTAACAGCT CTGAGTGTGG GCAGCAGCCA GCTGTGGAAA AGTGTGAGAC CATCCATGTG 
GCCATCGTCT GTGCCGGATA CAATGCCAGC AGGGACGTTG TCACCCTGGT GAAATCTGTC 
CTCTTTCATA GGCGGAACCC TCTGCACTTC CACCTCATTG CTGATTCTAT TGCTGAGCAG 
ATCCTGGCTA CACTCTTCCA GACCTGGATG GTACCTGCAG TGCGAGTAGA CTTCTATAAT 
GCTGATGAGC TCAAGTCTGA AGTGTCCTGG ATTCCCAACA AACATTACTC TGGAATTTAT 
GGCCTGATGA AGCTGGTTCT GACCAAAACT CTGCCTGCCA ACCTGGAGAG AGTCATTGTC 
CTAGACACCG ACATCACCTT TGCAACAGAC ATTGCAGAGC TGTGGGCCGT GTTCCATAAG 
TTCAAAGGGC AACAGGTCCT GGGCTTGGTA GAGAACCAGA GTGACTGGTA TCTTGGAAAT 
CTCTGGAAAA ACCACCGTCC ATGGCCAGCT CTTGGAAGGG GCTATAACAC AGGAGTCATA 
CTACTGCTTC TGGACAAGCT TCGGAAGATG AAATGGGAGC AGATGTGGAG ACTGACAGCA 
GAGAGGGAAC TCATGGGCAT GTTGTCTACA TCCTTGGCTG ACCAGGATAT TTTCAATGCT 
GTTATCAAAC AAAACCCCTT TCTGGTGTAT CAGCTTCCCT GCTTCTGGAA TGTGCAGCTG 
TCAGACCACA CCCGCTCTGA GCAGTGCTAC AGAGATGTGT CTGATTTAAA GGTCATCCAC 
TGGAACTCCC CCAAGAAGCT CCGTGTGAAG AACAAACATG TGGAATTCTT CCGCAACCTC 
TACCTGACCT TCTTGGAGTA TGATGGCAAC CTCCTGCGGC GGGAGCTGTT TGGCTGCCCC 
AGTGAGACTG ATGTCAACAA TGAAAATCTT CAGAAGCAGC TGTCTGAGCT GGATGAGGAT 
GACTTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACAGAACCCA CCTGTACTTC 
CTGCACTACG AGTTTGAGCC TTCTGCTGAC AACACAGACG TTACCCTTGT TGCTCAGCTC 
TCCATGGACA GACTACAGAT GCTGGAGGCC ATCTGTAAAC ACTGGGAGGG ACCCATCAGC 
CTGGCCCTCT ACCTGTCAGA TGCAGAAGCC CAGCAGTTCC TCCGCTATGC CCAGGGATCC 
GAGGTGCTCA TGAGCCGGCA GAACGTGGGC TACCACATCG TGTACAAGGA GGGTCAGTTC 
TATCCTGTCA ATCTTCTGCG AAACGTGGCT ATGAAGCACA TCAGCACACC CTACATGTTC 
TTATCTGACA TTGACTTCCT GCCTATGTAT GGGCTCTATG AGTACCTCAG GAAATCTGTC 
ATCCAGCTTG ACCTTGCCAA TACCAAGAAA GCAATGATTG TCCCAGCATT TGAAACGCTG 
CGCTACAGAC TCTCTTTCCC CAAGTCAAAA GCAGAGCTGC TGTCAATGTT GGACATGGGG 
ACCCTATTCA CATTCAGGTA TCACGTATGG ACTAAAGGCC ATGCACCCAC AAACTTTGCC 
AAGTGGCGGA CTGCCACCAC ACCTTACCAG GTTGAGTGGG AGGCTGATTT TGAGCCATAT 
GTTGTTGTGA GACGAGACTG CCCTGAGTAT GATCGGAGAT TTGTGGGCTT TGGCTGGAAC 
AAAGTGGCTC ACATCATGGA ATTGGATGCA CAGGAGTATG AGTTCACCGT GTTGCCCAAT 
GCCTACATGA TCCACATGCC CCATGCCCCT AGTTTCGACA TCACTAAGTT CCGTTCCAAC 
AAGCAATACC GCATCTGTCT CAAAACCCTG AAGGAAGAGT TTCAACAAGA CATGTCCCGA 
CGATATGGCT TTGCTGCCTT GAAATATCTC ACAGCTGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_034817.1

Translation

MLGICRGRRKFLAASLTLLCIPAITWIYLFAGSFEDGKPVSLSPLESQAHSPRYTASSQRERESLEVRVREVEEENRALRRQLSLAQGQSPAHHRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPAVEKCETIHVAIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSWIPNKHYSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGLVENQSDWYLGNLWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAERELMGMLSTSLADQDIFNAVIKQNPFLVYQLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRRELFGCPSETDVNNENLQKQLSELDEDDLCYEFRRERFTVHRTHLYFLHYEFEPSADNTDVTLVAQLSMDRLQMLEAICKHWEGPISLALYLSDAEAQQFLRYAQGSEVLMSRQNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMFLSDIDFLPMYGLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFTFRYHVWTKGHAPTNFAKWRTATTPYQVEWEADFEPYVVVRRDCPEYDRRFVGFGWNKVAHIMELDAQEYEFTVLPNAYMIHMPHAPSFDITKFRSNKQYRICLKTLKEEFQQDMSRRYGFAALKYLTAENNS

Target ORF information:

RefSeq Version	NM_010687.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus like-glycosyltransferase (Large), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010687.1

ORF Insert Sequence:

ATGCTGGGAA TCTGCAGAGG GAGAAGGAAA TTCCTGGCTG CCTCTTTGAC TCTACTCTGC 
ATACCAGCCA TCACCTGGAT TTACTTGTTT GCTGGGAGCT TTGAAGATGG GAAACCTGTG 
TCTCTGTCTC CCCTGGAGTC CCAAGCCCAC AGTCCCCGGT ACACAGCCTC CAGCCAACGG 
GAGCGGGAGA GCCTGGAAGT GCGTGTTCGA GAAGTTGAGG AGGAAAACCG TGCTCTGCGC 
AGGCAGCTCA GCCTGGCCCA GGGTCAGAGC CCTGCTCACC ACCGTGGGAA CCACTCCAAG 
ACCTACTCCA TGGAGGAGGG GACAGGGGAC AGTGAGAATC TGCGAGCTGG CATTGTGGCA 
GGTAACAGCT CTGAGTGTGG GCAGCAGCCA GCTGTGGAAA AGTGTGAGAC CATCCATGTG 
GCCATCGTCT GTGCCGGATA CAATGCCAGC AGGGACGTTG TCACCCTGGT GAAATCTGTC 
CTCTTTCATA GGCGGAACCC TCTGCACTTC CACCTCATTG CTGATTCTAT TGCTGAGCAG 
ATCCTGGCTA CACTCTTCCA GACCTGGATG GTACCTGCAG TGCGAGTAGA CTTCTATAAT 
GCTGATGAGC TCAAGTCTGA AGTGTCCTGG ATTCCCAACA AACATTACTC TGGAATTTAT 
GGCCTGATGA AGCTGGTTCT GACCAAAACT CTGCCTGCCA ACCTGGAGAG AGTCATTGTC 
CTAGACACCG ACATCACCTT TGCAACAGAC ATTGCAGAGC TGTGGGCCGT GTTCCATAAG 
TTCAAAGGGC AACAGGTCCT GGGCTTGGTA GAGAACCAGA GTGACTGGTA TCTTGGAAAT 
CTCTGGAAAA ACCACCGTCC ATGGCCAGCT CTTGGAAGGG GCTATAACAC AGGAGTCATA 
CTACTGCTTC TGGACAAGCT TCGGAAGATG AAATGGGAGC AGATGTGGAG ACTGACAGCA 
GAGAGGGAAC TCATGGGCAT GTTGTCTACA TCCTTGGCTG ACCAGGATAT TTTCAATGCT 
GTTATCAAAC AAAACCCCTT TCTGGTGTAT CAGCTTCCCT GCTTCTGGAA TGTGCAGCTG 
TCAGACCACA CCCGCTCTGA GCAGTGCTAC AGAGATGTGT CTGATTTAAA GGTCATCCAC 
TGGAACTCCC CCAAGAAGCT CCGTGTGAAG AACAAACATG TGGAATTCTT CCGCAACCTC 
TACCTGACCT TCTTGGAGTA TGATGGCAAC CTCCTGCGGC GGGAGCTGTT TGGCTGCCCC 
AGTGAGACTG ATGTCAACAA TGAAAATCTT CAGAAGCAGC TGTCTGAGCT GGATGAGGAT 
GACTTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACAGAACCCA CCTGTACTTC 
CTGCACTACG AGTTTGAGCC TTCTGCTGAC AACACAGACG TTACCCTTGT TGCTCAGCTC 
TCCATGGACA GACTACAGAT GCTGGAGGCC ATCTGTAAAC ACTGGGAGGG ACCCATCAGC 
CTGGCCCTCT ACCTGTCAGA TGCAGAAGCC CAGCAGTTCC TCCGCTATGC CCAGGGATCC 
GAGGTGCTCA TGAGCCGGCA GAACGTGGGC TACCACATCG TGTACAAGGA GGGTCAGTTC 
TATCCTGTCA ATCTTCTGCG AAACGTGGCT ATGAAGCACA TCAGCACACC CTACATGTTC 
TTATCTGACA TTGACTTCCT GCCTATGTAT GGGCTCTATG AGTACCTCAG GAAATCTGTC 
ATCCAGCTTG ACCTTGCCAA TACCAAGAAA GCAATGATTG TCCCAGCATT TGAAACGCTG 
CGCTACAGAC TCTCTTTCCC CAAGTCAAAA GCAGAGCTGC TGTCAATGTT GGACATGGGG 
ACCCTATTCA CATTCAGGTA TCACGTATGG ACTAAAGGCC ATGCACCCAC AAACTTTGCC 
AAGTGGCGGA CTGCCACCAC ACCTTACCAG GTTGAGTGGG AGGCTGATTT TGAGCCATAT 
GTTGTTGTGA GACGAGACTG CCCTGAGTAT GATCGGAGAT TTGTGGGCTT TGGCTGGAAC 
AAAGTGGCTC ACATCATGGA ATTGGATGCA CAGGAGTATG AGTTCACCGT GTTGCCCAAT 
GCCTACATGA TCCACATGCC CCATGCCCCT AGTTTCGACA TCACTAAGTT CCGTTCCAAC 
AAGCAATACC GCATCTGTCT CAAAACCCTG AAGGAAGAGT TTCAACAAGA CATGTCCCGA 
CGATATGGCT TTGCTGCCTT GAAATATCTC ACAGCTGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Large1 ( NM_001317391.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001304320.1 Mus musculus LARGE xylosyl- and glucuronyltransferase 1 (Large1), transcript variant 1, mRNA.

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CloneID	OMu18580
Clone ID Related Accession (Same CDS sequence)	XM_011248317.1 , NM_010687.2 , NM_010687.1 , NM_001317391.1
Accession Version	NM_001317391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	LARGE xylosyl- and glucuronyltransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157365.5, AC158562.8, AC115798.14, AC171678.8 and AC171917.14. On Dec 3, 2015 this sequence version replaced XM_011248317.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122328.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGGAA TCTGCAGAGG GAGAAGGAAA TTCCTGGCTG CCTCTTTGAC TCTACTCTGC 
ATACCAGCCA TCACCTGGAT TTACTTGTTT GCTGGGAGCT TTGAAGATGG GAAACCTGTG 
TCTCTGTCTC CCCTGGAGTC CCAAGCCCAC AGTCCCCGGT ACACAGCCTC CAGCCAACGG 
GAGCGGGAGA GCCTGGAAGT GCGTGTTCGA GAAGTTGAGG AGGAAAACCG TGCTCTGCGC 
AGGCAGCTCA GCCTGGCCCA GGGTCAGAGC CCTGCTCACC ACCGTGGGAA CCACTCCAAG 
ACCTACTCCA TGGAGGAGGG GACAGGGGAC AGTGAGAATC TGCGAGCTGG CATTGTGGCA 
GGTAACAGCT CTGAGTGTGG GCAGCAGCCA GCTGTGGAAA AGTGTGAGAC CATCCATGTG 
GCCATCGTCT GTGCCGGATA CAATGCCAGC AGGGACGTTG TCACCCTGGT GAAATCTGTC 
CTCTTTCATA GGCGGAACCC TCTGCACTTC CACCTCATTG CTGATTCTAT TGCTGAGCAG 
ATCCTGGCTA CACTCTTCCA GACCTGGATG GTACCTGCAG TGCGAGTAGA CTTCTATAAT 
GCTGATGAGC TCAAGTCTGA AGTGTCCTGG ATTCCCAACA AACATTACTC TGGAATTTAT 
GGCCTGATGA AGCTGGTTCT GACCAAAACT CTGCCTGCCA ACCTGGAGAG AGTCATTGTC 
CTAGACACCG ACATCACCTT TGCAACAGAC ATTGCAGAGC TGTGGGCCGT GTTCCATAAG 
TTCAAAGGGC AACAGGTCCT GGGCTTGGTA GAGAACCAGA GTGACTGGTA TCTTGGAAAT 
CTCTGGAAAA ACCACCGTCC ATGGCCAGCT CTTGGAAGGG GCTATAACAC AGGAGTCATA 
CTACTGCTTC TGGACAAGCT TCGGAAGATG AAATGGGAGC AGATGTGGAG ACTGACAGCA 
GAGAGGGAAC TCATGGGCAT GTTGTCTACA TCCTTGGCTG ACCAGGATAT TTTCAATGCT 
GTTATCAAAC AAAACCCCTT TCTGGTGTAT CAGCTTCCCT GCTTCTGGAA TGTGCAGCTG 
TCAGACCACA CCCGCTCTGA GCAGTGCTAC AGAGATGTGT CTGATTTAAA GGTCATCCAC 
TGGAACTCCC CCAAGAAGCT CCGTGTGAAG AACAAACATG TGGAATTCTT CCGCAACCTC 
TACCTGACCT TCTTGGAGTA TGATGGCAAC CTCCTGCGGC GGGAGCTGTT TGGCTGCCCC 
AGTGAGACTG ATGTCAACAA TGAAAATCTT CAGAAGCAGC TGTCTGAGCT GGATGAGGAT 
GACTTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACAGAACCCA CCTGTACTTC 
CTGCACTACG AGTTTGAGCC TTCTGCTGAC AACACAGACG TTACCCTTGT TGCTCAGCTC 
TCCATGGACA GACTACAGAT GCTGGAGGCC ATCTGTAAAC ACTGGGAGGG ACCCATCAGC 
CTGGCCCTCT ACCTGTCAGA TGCAGAAGCC CAGCAGTTCC TCCGCTATGC CCAGGGATCC 
GAGGTGCTCA TGAGCCGGCA GAACGTGGGC TACCACATCG TGTACAAGGA GGGTCAGTTC 
TATCCTGTCA ATCTTCTGCG AAACGTGGCT ATGAAGCACA TCAGCACACC CTACATGTTC 
TTATCTGACA TTGACTTCCT GCCTATGTAT GGGCTCTATG AGTACCTCAG GAAATCTGTC 
ATCCAGCTTG ACCTTGCCAA TACCAAGAAA GCAATGATTG TCCCAGCATT TGAAACGCTG 
CGCTACAGAC TCTCTTTCCC CAAGTCAAAA GCAGAGCTGC TGTCAATGTT GGACATGGGG 
ACCCTATTCA CATTCAGGTA TCACGTATGG ACTAAAGGCC ATGCACCCAC AAACTTTGCC 
AAGTGGCGGA CTGCCACCAC ACCTTACCAG GTTGAGTGGG AGGCTGATTT TGAGCCATAT 
GTTGTTGTGA GACGAGACTG CCCTGAGTAT GATCGGAGAT TTGTGGGCTT TGGCTGGAAC 
AAAGTGGCTC ACATCATGGA ATTGGATGCA CAGGAGTATG AGTTCACCGT GTTGCCCAAT 
GCCTACATGA TCCACATGCC CCATGCCCCT AGTTTCGACA TCACTAAGTT CCGTTCCAAC 
AAGCAATACC GCATCTGTCT CAAAACCCTG AAGGAAGAGT TTCAACAAGA CATGTCCCGA 
CGATATGGCT TTGCTGCCTT GAAATATCTC ACAGCTGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304320.1
CDS	1251..3521
Translation	MLGICRGRRKFLAASLTLLCIPAITWIYLFAGSFEDGKPVSLSPLESQAHSPRYTASSQRERESLEVRVREVEEENRALRRQLSLAQGQSPAHHRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPAVEKCETIHVAIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSWIPNKHYSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGLVENQSDWYLGNLWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAERELMGMLSTSLADQDIFNAVIKQNPFLVYQLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRRELFGCPSETDVNNENLQKQLSELDEDDLCYEFRRERFTVHRTHLYFLHYEFEPSADNTDVTLVAQLSMDRLQMLEAICKHWEGPISLALYLSDAEAQQFLRYAQGSEVLMSRQNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMFLSDIDFLPMYGLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFTFRYHVWTKGHAPTNFAKWRTATTPYQVEWEADFEPYVVVRRDCPEYDRRFVGFGWNKVAHIMELDAQEYEFTVLPNAYMIHMPHAPSFDITKFRSNKQYRICLKTLKEEFQQDMSRRYGFAALKYLTAENNS

Target ORF information:

RefSeq Version	NM_001317391.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LARGE xylosyl- and glucuronyltransferase 1 (Large1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317391.1

ORF Insert Sequence:

ATGCTGGGAA TCTGCAGAGG GAGAAGGAAA TTCCTGGCTG CCTCTTTGAC TCTACTCTGC 
ATACCAGCCA TCACCTGGAT TTACTTGTTT GCTGGGAGCT TTGAAGATGG GAAACCTGTG 
TCTCTGTCTC CCCTGGAGTC CCAAGCCCAC AGTCCCCGGT ACACAGCCTC CAGCCAACGG 
GAGCGGGAGA GCCTGGAAGT GCGTGTTCGA GAAGTTGAGG AGGAAAACCG TGCTCTGCGC 
AGGCAGCTCA GCCTGGCCCA GGGTCAGAGC CCTGCTCACC ACCGTGGGAA CCACTCCAAG 
ACCTACTCCA TGGAGGAGGG GACAGGGGAC AGTGAGAATC TGCGAGCTGG CATTGTGGCA 
GGTAACAGCT CTGAGTGTGG GCAGCAGCCA GCTGTGGAAA AGTGTGAGAC CATCCATGTG 
GCCATCGTCT GTGCCGGATA CAATGCCAGC AGGGACGTTG TCACCCTGGT GAAATCTGTC 
CTCTTTCATA GGCGGAACCC TCTGCACTTC CACCTCATTG CTGATTCTAT TGCTGAGCAG 
ATCCTGGCTA CACTCTTCCA GACCTGGATG GTACCTGCAG TGCGAGTAGA CTTCTATAAT 
GCTGATGAGC TCAAGTCTGA AGTGTCCTGG ATTCCCAACA AACATTACTC TGGAATTTAT 
GGCCTGATGA AGCTGGTTCT GACCAAAACT CTGCCTGCCA ACCTGGAGAG AGTCATTGTC 
CTAGACACCG ACATCACCTT TGCAACAGAC ATTGCAGAGC TGTGGGCCGT GTTCCATAAG 
TTCAAAGGGC AACAGGTCCT GGGCTTGGTA GAGAACCAGA GTGACTGGTA TCTTGGAAAT 
CTCTGGAAAA ACCACCGTCC ATGGCCAGCT CTTGGAAGGG GCTATAACAC AGGAGTCATA 
CTACTGCTTC TGGACAAGCT TCGGAAGATG AAATGGGAGC AGATGTGGAG ACTGACAGCA 
GAGAGGGAAC TCATGGGCAT GTTGTCTACA TCCTTGGCTG ACCAGGATAT TTTCAATGCT 
GTTATCAAAC AAAACCCCTT TCTGGTGTAT CAGCTTCCCT GCTTCTGGAA TGTGCAGCTG 
TCAGACCACA CCCGCTCTGA GCAGTGCTAC AGAGATGTGT CTGATTTAAA GGTCATCCAC 
TGGAACTCCC CCAAGAAGCT CCGTGTGAAG AACAAACATG TGGAATTCTT CCGCAACCTC 
TACCTGACCT TCTTGGAGTA TGATGGCAAC CTCCTGCGGC GGGAGCTGTT TGGCTGCCCC 
AGTGAGACTG ATGTCAACAA TGAAAATCTT CAGAAGCAGC TGTCTGAGCT GGATGAGGAT 
GACTTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACAGAACCCA CCTGTACTTC 
CTGCACTACG AGTTTGAGCC TTCTGCTGAC AACACAGACG TTACCCTTGT TGCTCAGCTC 
TCCATGGACA GACTACAGAT GCTGGAGGCC ATCTGTAAAC ACTGGGAGGG ACCCATCAGC 
CTGGCCCTCT ACCTGTCAGA TGCAGAAGCC CAGCAGTTCC TCCGCTATGC CCAGGGATCC 
GAGGTGCTCA TGAGCCGGCA GAACGTGGGC TACCACATCG TGTACAAGGA GGGTCAGTTC 
TATCCTGTCA ATCTTCTGCG AAACGTGGCT ATGAAGCACA TCAGCACACC CTACATGTTC 
TTATCTGACA TTGACTTCCT GCCTATGTAT GGGCTCTATG AGTACCTCAG GAAATCTGTC 
ATCCAGCTTG ACCTTGCCAA TACCAAGAAA GCAATGATTG TCCCAGCATT TGAAACGCTG 
CGCTACAGAC TCTCTTTCCC CAAGTCAAAA GCAGAGCTGC TGTCAATGTT GGACATGGGG 
ACCCTATTCA CATTCAGGTA TCACGTATGG ACTAAAGGCC ATGCACCCAC AAACTTTGCC 
AAGTGGCGGA CTGCCACCAC ACCTTACCAG GTTGAGTGGG AGGCTGATTT TGAGCCATAT 
GTTGTTGTGA GACGAGACTG CCCTGAGTAT GATCGGAGAT TTGTGGGCTT TGGCTGGAAC 
AAAGTGGCTC ACATCATGGA ATTGGATGCA CAGGAGTATG AGTTCACCGT GTTGCCCAAT 
GCCTACATGA TCCACATGCC CCATGCCCCT AGTTTCGACA TCACTAAGTT CCGTTCCAAC 
AAGCAATACC GCATCTGTCT CAAAACCCTG AAGGAAGAGT TTCAACAAGA CATGTCCCGA 
CGATATGGCT TTGCTGCCTT GAAATATCTC ACAGCTGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Large1 ( XM_011248317.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246619.1 Mus musculus like-glycosyltransferase (Large), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18580
Clone ID Related Accession (Same CDS sequence)	XM_011248317.1 , NM_010687.2 , NM_010687.1 , NM_001317391.1
Accession Version	XM_011248317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	glycosyltransferase-like protein LARGE1 isoform X1
Comment

ATGCTGGGAA TCTGCAGAGG GAGAAGGAAA TTCCTGGCTG CCTCTTTGAC TCTACTCTGC 
ATACCAGCCA TCACCTGGAT TTACTTGTTT GCTGGGAGCT TTGAAGATGG GAAACCTGTG 
TCTCTGTCTC CCCTGGAGTC CCAAGCCCAC AGTCCCCGGT ACACAGCCTC CAGCCAACGG 
GAGCGGGAGA GCCTGGAAGT GCGTGTTCGA GAAGTTGAGG AGGAAAACCG TGCTCTGCGC 
AGGCAGCTCA GCCTGGCCCA GGGTCAGAGC CCTGCTCACC ACCGTGGGAA CCACTCCAAG 
ACCTACTCCA TGGAGGAGGG GACAGGGGAC AGTGAGAATC TGCGAGCTGG CATTGTGGCA 
GGTAACAGCT CTGAGTGTGG GCAGCAGCCA GCTGTGGAAA AGTGTGAGAC CATCCATGTG 
GCCATCGTCT GTGCCGGATA CAATGCCAGC AGGGACGTTG TCACCCTGGT GAAATCTGTC 
CTCTTTCATA GGCGGAACCC TCTGCACTTC CACCTCATTG CTGATTCTAT TGCTGAGCAG 
ATCCTGGCTA CACTCTTCCA GACCTGGATG GTACCTGCAG TGCGAGTAGA CTTCTATAAT 
GCTGATGAGC TCAAGTCTGA AGTGTCCTGG ATTCCCAACA AACATTACTC TGGAATTTAT 
GGCCTGATGA AGCTGGTTCT GACCAAAACT CTGCCTGCCA ACCTGGAGAG AGTCATTGTC 
CTAGACACCG ACATCACCTT TGCAACAGAC ATTGCAGAGC TGTGGGCCGT GTTCCATAAG 
TTCAAAGGGC AACAGGTCCT GGGCTTGGTA GAGAACCAGA GTGACTGGTA TCTTGGAAAT 
CTCTGGAAAA ACCACCGTCC ATGGCCAGCT CTTGGAAGGG GCTATAACAC AGGAGTCATA 
CTACTGCTTC TGGACAAGCT TCGGAAGATG AAATGGGAGC AGATGTGGAG ACTGACAGCA 
GAGAGGGAAC TCATGGGCAT GTTGTCTACA TCCTTGGCTG ACCAGGATAT TTTCAATGCT 
GTTATCAAAC AAAACCCCTT TCTGGTGTAT CAGCTTCCCT GCTTCTGGAA TGTGCAGCTG 
TCAGACCACA CCCGCTCTGA GCAGTGCTAC AGAGATGTGT CTGATTTAAA GGTCATCCAC 
TGGAACTCCC CCAAGAAGCT CCGTGTGAAG AACAAACATG TGGAATTCTT CCGCAACCTC 
TACCTGACCT TCTTGGAGTA TGATGGCAAC CTCCTGCGGC GGGAGCTGTT TGGCTGCCCC 
AGTGAGACTG ATGTCAACAA TGAAAATCTT CAGAAGCAGC TGTCTGAGCT GGATGAGGAT 
GACTTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACAGAACCCA CCTGTACTTC 
CTGCACTACG AGTTTGAGCC TTCTGCTGAC AACACAGACG TTACCCTTGT TGCTCAGCTC 
TCCATGGACA GACTACAGAT GCTGGAGGCC ATCTGTAAAC ACTGGGAGGG ACCCATCAGC 
CTGGCCCTCT ACCTGTCAGA TGCAGAAGCC CAGCAGTTCC TCCGCTATGC CCAGGGATCC 
GAGGTGCTCA TGAGCCGGCA GAACGTGGGC TACCACATCG TGTACAAGGA GGGTCAGTTC 
TATCCTGTCA ATCTTCTGCG AAACGTGGCT ATGAAGCACA TCAGCACACC CTACATGTTC 
TTATCTGACA TTGACTTCCT GCCTATGTAT GGGCTCTATG AGTACCTCAG GAAATCTGTC 
ATCCAGCTTG ACCTTGCCAA TACCAAGAAA GCAATGATTG TCCCAGCATT TGAAACGCTG 
CGCTACAGAC TCTCTTTCCC CAAGTCAAAA GCAGAGCTGC TGTCAATGTT GGACATGGGG 
ACCCTATTCA CATTCAGGTA TCACGTATGG ACTAAAGGCC ATGCACCCAC AAACTTTGCC 
AAGTGGCGGA CTGCCACCAC ACCTTACCAG GTTGAGTGGG AGGCTGATTT TGAGCCATAT 
GTTGTTGTGA GACGAGACTG CCCTGAGTAT GATCGGAGAT TTGTGGGCTT TGGCTGGAAC 
AAAGTGGCTC ACATCATGGA ATTGGATGCA CAGGAGTATG AGTTCACCGT GTTGCCCAAT 
GCCTACATGA TCCACATGCC CCATGCCCCT AGTTTCGACA TCACTAAGTT CCGTTCCAAC 
AAGCAATACC GCATCTGTCT CAAAACCCTG AAGGAAGAGT TTCAACAAGA CATGTCCCGA 
CGATATGGCT TTGCTGCCTT GAAATATCTC ACAGCTGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011246619.1

Translation

MLGICRGRRKFLAASLTLLCIPAITWIYLFAGSFEDGKPVSLSPLESQAHSPRYTASSQRERESLEVRVREVEEENRALRRQLSLAQGQSPAHHRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPAVEKCETIHVAIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSWIPNKHYSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGLVENQSDWYLGNLWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAERELMGMLSTSLADQDIFNAVIKQNPFLVYQLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRRELFGCPSETDVNNENLQKQLSELDEDDLCYEFRRERFTVHRTHLYFLHYEFEPSADNTDVTLVAQLSMDRLQMLEAICKHWEGPISLALYLSDAEAQQFLRYAQGSEVLMSRQNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMFLSDIDFLPMYGLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFTFRYHVWTKGHAPTNFAKWRTATTPYQVEWEADFEPYVVVRRDCPEYDRRFVGFGWNKVAHIMELDAQEYEFTVLPNAYMIHMPHAPSFDITKFRSNKQYRICLKTLKEEFQQDMSRRYGFAALKYLTAENNS

Target ORF information:

RefSeq Version	XM_011248317.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus like-glycosyltransferase (Large), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248317.1

ORF Insert Sequence:

ATGCTGGGAA TCTGCAGAGG GAGAAGGAAA TTCCTGGCTG CCTCTTTGAC TCTACTCTGC 
ATACCAGCCA TCACCTGGAT TTACTTGTTT GCTGGGAGCT TTGAAGATGG GAAACCTGTG 
TCTCTGTCTC CCCTGGAGTC CCAAGCCCAC AGTCCCCGGT ACACAGCCTC CAGCCAACGG 
GAGCGGGAGA GCCTGGAAGT GCGTGTTCGA GAAGTTGAGG AGGAAAACCG TGCTCTGCGC 
AGGCAGCTCA GCCTGGCCCA GGGTCAGAGC CCTGCTCACC ACCGTGGGAA CCACTCCAAG 
ACCTACTCCA TGGAGGAGGG GACAGGGGAC AGTGAGAATC TGCGAGCTGG CATTGTGGCA 
GGTAACAGCT CTGAGTGTGG GCAGCAGCCA GCTGTGGAAA AGTGTGAGAC CATCCATGTG 
GCCATCGTCT GTGCCGGATA CAATGCCAGC AGGGACGTTG TCACCCTGGT GAAATCTGTC 
CTCTTTCATA GGCGGAACCC TCTGCACTTC CACCTCATTG CTGATTCTAT TGCTGAGCAG 
ATCCTGGCTA CACTCTTCCA GACCTGGATG GTACCTGCAG TGCGAGTAGA CTTCTATAAT 
GCTGATGAGC TCAAGTCTGA AGTGTCCTGG ATTCCCAACA AACATTACTC TGGAATTTAT 
GGCCTGATGA AGCTGGTTCT GACCAAAACT CTGCCTGCCA ACCTGGAGAG AGTCATTGTC 
CTAGACACCG ACATCACCTT TGCAACAGAC ATTGCAGAGC TGTGGGCCGT GTTCCATAAG 
TTCAAAGGGC AACAGGTCCT GGGCTTGGTA GAGAACCAGA GTGACTGGTA TCTTGGAAAT 
CTCTGGAAAA ACCACCGTCC ATGGCCAGCT CTTGGAAGGG GCTATAACAC AGGAGTCATA 
CTACTGCTTC TGGACAAGCT TCGGAAGATG AAATGGGAGC AGATGTGGAG ACTGACAGCA 
GAGAGGGAAC TCATGGGCAT GTTGTCTACA TCCTTGGCTG ACCAGGATAT TTTCAATGCT 
GTTATCAAAC AAAACCCCTT TCTGGTGTAT CAGCTTCCCT GCTTCTGGAA TGTGCAGCTG 
TCAGACCACA CCCGCTCTGA GCAGTGCTAC AGAGATGTGT CTGATTTAAA GGTCATCCAC 
TGGAACTCCC CCAAGAAGCT CCGTGTGAAG AACAAACATG TGGAATTCTT CCGCAACCTC 
TACCTGACCT TCTTGGAGTA TGATGGCAAC CTCCTGCGGC GGGAGCTGTT TGGCTGCCCC 
AGTGAGACTG ATGTCAACAA TGAAAATCTT CAGAAGCAGC TGTCTGAGCT GGATGAGGAT 
GACTTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACAGAACCCA CCTGTACTTC 
CTGCACTACG AGTTTGAGCC TTCTGCTGAC AACACAGACG TTACCCTTGT TGCTCAGCTC 
TCCATGGACA GACTACAGAT GCTGGAGGCC ATCTGTAAAC ACTGGGAGGG ACCCATCAGC 
CTGGCCCTCT ACCTGTCAGA TGCAGAAGCC CAGCAGTTCC TCCGCTATGC CCAGGGATCC 
GAGGTGCTCA TGAGCCGGCA GAACGTGGGC TACCACATCG TGTACAAGGA GGGTCAGTTC 
TATCCTGTCA ATCTTCTGCG AAACGTGGCT ATGAAGCACA TCAGCACACC CTACATGTTC 
TTATCTGACA TTGACTTCCT GCCTATGTAT GGGCTCTATG AGTACCTCAG GAAATCTGTC 
ATCCAGCTTG ACCTTGCCAA TACCAAGAAA GCAATGATTG TCCCAGCATT TGAAACGCTG 
CGCTACAGAC TCTCTTTCCC CAAGTCAAAA GCAGAGCTGC TGTCAATGTT GGACATGGGG 
ACCCTATTCA CATTCAGGTA TCACGTATGG ACTAAAGGCC ATGCACCCAC AAACTTTGCC 
AAGTGGCGGA CTGCCACCAC ACCTTACCAG GTTGAGTGGG AGGCTGATTT TGAGCCATAT 
GTTGTTGTGA GACGAGACTG CCCTGAGTAT GATCGGAGAT TTGTGGGCTT TGGCTGGAAC 
AAAGTGGCTC ACATCATGGA ATTGGATGCA CAGGAGTATG AGTTCACCGT GTTGCCCAAT 
GCCTACATGA TCCACATGCC CCATGCCCCT AGTTTCGACA TCACTAAGTT CCGTTCCAAC 
AAGCAATACC GCATCTGTCT CAAAACCCTG AAGGAAGAGT TTCAACAAGA CATGTCCCGA 
CGATATGGCT TTGCTGCCTT GAAATATCTC ACAGCTGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Large1 ( XM_030243312.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099172.1 Mus musculus LARGE xylosyl- and glucuronyltransferase 1 (Large1), transcript variant X1, mRNA.

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CloneID	OMu89752
Clone ID Related Accession (Same CDS sequence)	XM_030243312.1
Accession Version	XM_030243312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	LARGE xylosyl- and glucuronyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GGACAGGGGA CAGTGAGAAT CTGCGAGCTG GCATTGTGGC AGGTAACAGC 
TCTGAGTGTG GGCAGCAGCC AGCTGTGGAA AAGTGTGAGA CCATCCATGT GGCCATCGTC 
TGTGCCGGAT ACAATGCCAG CAGGGACGTT GTCACCCTGG TGAAATCTGT CCTCTTTCAT 
AGGCGGAACC CTCTGCACTT CCACCTCATT GCTGATTCTA TTGCTGAGCA GATCCTGGCT 
ACACTCTTCC AGACCTGGAT GGTACCTGCA GTGCGAGTAG ACTTCTATAA TGCTGATGAG 
CTCAAGTCTG AAGTGTCCTG GATTCCCAAC AAACATTACT CTGGAATTTA TGGCCTGATG 
AAGCTGGTTC TGACCAAAAC TCTGCCTGCC AACCTGGAGA GAGTCATTGT CCTAGACACC 
GACATCACCT TTGCAACAGA CATTGCAGAG CTGTGGGCCG TGTTCCATAA GTTCAAAGGG 
CAACAGGTCC TGGGCTTGGT AGAGAACCAG AGTGACTGGT ATCTTGGAAA TCTCTGGAAA 
AACCACCGTC CATGGCCAGC TCTTGGAAGG GGCTATAACA CAGGAGTCAT ACTACTGCTT 
CTGGACAAGC TTCGGAAGAT GAAATGGGAG CAGATGTGGA GACTGACAGC AGAGAGGGAA 
CTCATGGGCA TGTTGTCTAC ATCCTTGGCT GACCAGGATA TTTTCAATGC TGTTATCAAA 
CAAAACCCCT TTCTGGTGTA TCAGCTTCCC TGCTTCTGGA ATGTGCAGCT GTCAGACCAC 
ACCCGCTCTG AGCAGTGCTA CAGAGATGTG TCTGATTTAA AGGTCATCCA CTGGAACTCC 
CCCAAGAAGC TCCGTGTGAA GAACAAACAT GTGGAATTCT TCCGCAACCT CTACCTGACC 
TTCTTGGAGT ATGATGGCAA CCTCCTGCGG CGGGAGCTGT TTGGCTGCCC CAGTGAGACT 
GATGTCAACA ATGAAAATCT TCAGAAGCAG CTGTCTGAGC TGGATGAGGA TGACTTGTGC 
TATGAGTTCC GGCGAGAGCG CTTCACTGTC CACAGAACCC ACCTGTACTT CCTGCACTAC 
GAGTTTGAGC CTTCTGCTGA CAACACAGAC GTTACCCTTG TTGCTCAGCT CTCCATGGAC 
AGACTACAGA TGCTGGAGGC CATCTGTAAA CACTGGGAGG GACCCATCAG CCTGGCCCTC 
TACCTGTCAG ATGCAGAAGC CCAGCAGTTC CTCCGCTATG CCCAGGGATC CGAGGTGCTC 
ATGAGCCGGC AGAACGTGGG CTACCACATC GTGTACAAGG AGGGTCAGTT CTATCCTGTC 
AATCTTCTGC GAAACGTGGC TATGAAGCAC ATCAGCACAC CCTACATGTT CTTATCTGAC 
ATTGACTTCC TGCCTATGTA TGGGCTCTAT GAGTACCTCA GGAAATCTGT CATCCAGCTT 
GACCTTGCCA ATACCAAGAA AGCAATGATT GTCCCAGCAT TTGAAACGCT GCGCTACAGA 
CTCTCTTTCC CCAAGTCAAA AGCAGAGCTG CTGTCAATGT TGGACATGGG GACCCTATTC 
ACATTCAGGT ATCACGTATG GACTAAAGGC CATGCACCCA CAAACTTTGC CAAGTGGCGG 
ACTGCCACCA CACCTTACCA GGTTGAGTGG GAGGCTGATT TTGAGCCATA TGTTGTTGTG 
AGACGAGACT GCCCTGAGTA TGATCGGAGA TTTGTGGGCT TTGGCTGGAA CAAAGTGGCT 
CACATCATGG AATTGGATGC ACAGGAGTAT GAGTTCACCG TGTTGCCCAA TGCCTACATG 
ATCCACATGC CCCATGCCCC TAGTTTCGAC ATCACTAAGT TCCGTTCCAA CAAGCAATAC 
CGCATCTGTC TCAAAACCCT GAAGGAAGAG TTTCAACAAG ACATGTCCCG ACGATATGGC 
TTTGCTGCCT TGAAATATCT CACAGCTGAG AACAACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099172.1
CDS	260..2221
Translation	MEEGTGDSENLRAGIVAGNSSECGQQPAVEKCETIHVAIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSWIPNKHYSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGLVENQSDWYLGNLWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAERELMGMLSTSLADQDIFNAVIKQNPFLVYQLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRRELFGCPSETDVNNENLQKQLSELDEDDLCYEFRRERFTVHRTHLYFLHYEFEPSADNTDVTLVAQLSMDRLQMLEAICKHWEGPISLALYLSDAEAQQFLRYAQGSEVLMSRQNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMFLSDIDFLPMYGLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFTFRYHVWTKGHAPTNFAKWRTATTPYQVEWEADFEPYVVVRRDCPEYDRRFVGFGWNKVAHIMELDAQEYEFTVLPNAYMIHMPHAPSFDITKFRSNKQYRICLKTLKEEFQQDMSRRYGFAALKYLTAENNS

Target ORF information:

RefSeq Version	XM_030243312.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LARGE xylosyl- and glucuronyltransferase 1 (Large1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243312.1

ORF Insert Sequence:

ATGGAGGAGG GGACAGGGGA CAGTGAGAAT CTGCGAGCTG GCATTGTGGC AGGTAACAGC 
TCTGAGTGTG GGCAGCAGCC AGCTGTGGAA AAGTGTGAGA CCATCCATGT GGCCATCGTC 
TGTGCCGGAT ACAATGCCAG CAGGGACGTT GTCACCCTGG TGAAATCTGT CCTCTTTCAT 
AGGCGGAACC CTCTGCACTT CCACCTCATT GCTGATTCTA TTGCTGAGCA GATCCTGGCT 
ACACTCTTCC AGACCTGGAT GGTACCTGCA GTGCGAGTAG ACTTCTATAA TGCTGATGAG 
CTCAAGTCTG AAGTGTCCTG GATTCCCAAC AAACATTACT CTGGAATTTA TGGCCTGATG 
AAGCTGGTTC TGACCAAAAC TCTGCCTGCC AACCTGGAGA GAGTCATTGT CCTAGACACC 
GACATCACCT TTGCAACAGA CATTGCAGAG CTGTGGGCCG TGTTCCATAA GTTCAAAGGG 
CAACAGGTCC TGGGCTTGGT AGAGAACCAG AGTGACTGGT ATCTTGGAAA TCTCTGGAAA 
AACCACCGTC CATGGCCAGC TCTTGGAAGG GGCTATAACA CAGGAGTCAT ACTACTGCTT 
CTGGACAAGC TTCGGAAGAT GAAATGGGAG CAGATGTGGA GACTGACAGC AGAGAGGGAA 
CTCATGGGCA TGTTGTCTAC ATCCTTGGCT GACCAGGATA TTTTCAATGC TGTTATCAAA 
CAAAACCCCT TTCTGGTGTA TCAGCTTCCC TGCTTCTGGA ATGTGCAGCT GTCAGACCAC 
ACCCGCTCTG AGCAGTGCTA CAGAGATGTG TCTGATTTAA AGGTCATCCA CTGGAACTCC 
CCCAAGAAGC TCCGTGTGAA GAACAAACAT GTGGAATTCT TCCGCAACCT CTACCTGACC 
TTCTTGGAGT ATGATGGCAA CCTCCTGCGG CGGGAGCTGT TTGGCTGCCC CAGTGAGACT 
GATGTCAACA ATGAAAATCT TCAGAAGCAG CTGTCTGAGC TGGATGAGGA TGACTTGTGC 
TATGAGTTCC GGCGAGAGCG CTTCACTGTC CACAGAACCC ACCTGTACTT CCTGCACTAC 
GAGTTTGAGC CTTCTGCTGA CAACACAGAC GTTACCCTTG TTGCTCAGCT CTCCATGGAC 
AGACTACAGA TGCTGGAGGC CATCTGTAAA CACTGGGAGG GACCCATCAG CCTGGCCCTC 
TACCTGTCAG ATGCAGAAGC CCAGCAGTTC CTCCGCTATG CCCAGGGATC CGAGGTGCTC 
ATGAGCCGGC AGAACGTGGG CTACCACATC GTGTACAAGG AGGGTCAGTT CTATCCTGTC 
AATCTTCTGC GAAACGTGGC TATGAAGCAC ATCAGCACAC CCTACATGTT CTTATCTGAC 
ATTGACTTCC TGCCTATGTA TGGGCTCTAT GAGTACCTCA GGAAATCTGT CATCCAGCTT 
GACCTTGCCA ATACCAAGAA AGCAATGATT GTCCCAGCAT TTGAAACGCT GCGCTACAGA 
CTCTCTTTCC CCAAGTCAAA AGCAGAGCTG CTGTCAATGT TGGACATGGG GACCCTATTC 
ACATTCAGGT ATCACGTATG GACTAAAGGC CATGCACCCA CAAACTTTGC CAAGTGGCGG 
ACTGCCACCA CACCTTACCA GGTTGAGTGG GAGGCTGATT TTGAGCCATA TGTTGTTGTG 
AGACGAGACT GCCCTGAGTA TGATCGGAGA TTTGTGGGCT TTGGCTGGAA CAAAGTGGCT 
CACATCATGG AATTGGATGC ACAGGAGTAT GAGTTCACCG TGTTGCCCAA TGCCTACATG 
ATCCACATGC CCCATGCCCC TAGTTTCGAC ATCACTAAGT TCCGTTCCAA CAAGCAATAC 
CGCATCTGTC TCAAAACCCT GAAGGAAGAG TTTCAACAAG ACATGTCCCG ACGATATGGC 
TTTGCTGCCT TGAAATATCT CACAGCTGAG AACAACAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Degree of Cajal-Retzius Cell Mislocalization Correlates with the Severity of Structural Brain Defects in Mouse Models of Dystroglycanopathy.
Brain pathology (Zurich, Switzerland)26(4)465-78(2016 Jul)
Booler HS,Williams JL,Hopkinson M,Brown SC

Transgenic Rescue of the LARGEmyd Mouse: A LARGE Therapeutic Window?
PloS one11(7)e0159853(2016)
Hildyard JC,Lacey E,Booler H,Hopkinson M,Wells DJ,Brown SC

Restoration of Functional Glycosylation of ?-Dystroglycan in FKRP Mutant Mice Is Associated with Muscle Regeneration.
The American journal of pathology185(7)2025-37(2015 Jul)
Awano H,Blaeser A,Keramaris E,Xu L,Tucker J,Wu B,Lu P,Lu QL

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Large1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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