Kif4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kif4
Entrez Gene ID	16571
Full Name	kinesin family member 4
Synonyms	AI323435,D330050K22Rik,Kns4
General protein information	Preferred Names kinesin family member 4 Names chromosome-associated kinesin KIF4 N-5 kinesin chromokinesin kinesin heavy chain member 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X C3\|X 43.72 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527855.3	XP_006527918.1	chromosome-associated kinesin KIF4 isoform X1
XM_006527855.4	XP_006527918.1	chromosome-associated kinesin KIF4 isoform X1
XM_011247528.2	XP_011245830.1	chromosome-associated kinesin KIF4 isoform X2
XM_017318397.1	XP_017173886.1	chromosome-associated kinesin KIF4 isoform X3
XM_017318397.2	XP_017173886.1	chromosome-associated kinesin KIF4 isoform X3
XM_011247528.3	XP_011245830.1	chromosome-associated kinesin KIF4 isoform X2
NM_008446.2	NP_032472.2	chromosome-associated kinesin KIF4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kif4a	XP_006257175.1
Danio rerio (zebrafish)	zgc:66125	NP_957117.1
Homo sapiens (human)	KIF4A	NP_036442.3
Arabidopsis thaliana (thale cress)	AT5G60930	NP_200901.2
Xenopus tropicalis (tropical clawed frog)	LOC100494653	XP_004916888.1
Drosophila melanogaster (fruit fly)	Klp3A	NP_525053.1
Canis lupus familiaris (dog)	KIF4A	XP_549061.2
Bos taurus (cattle)	KIF4A	NP_001193034.1
Gallus gallus (chicken)	KIF4A	NP_990306.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000575	XP_003437036.1
Mus musculus (house mouse)	Kif4	NP_032472.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012471	XP_307305.3
Caenorhabditis elegans (roundworm)	klp-19	NP_499742.1
Xenopus tropicalis (tropical clawed frog)	kif4a	NP_001006741.1

Gene Ontology
Bibliography

Related articles in PubMed

Contribution of epigenetic landscapes and transcription factors to X-chromosome reactivation in the inner cell mass.
Borensztein M, Okamoto I, Syx L, Guilbaud G, Picard C, Ancelin K, Galupa R, Diabangouaya P, Servant N, Barillot E, Surani A, Saitou M, Chen CJ, Anastassiadis K, Heard E
Nature communications8(1)1297(2017 Nov)

Kif4 regulates the expression of VEGFR1 through the PI3K/Akt signaling pathway in RAW264.7 monocytes/macrophages.
Xu Y, Luan Y, Liu S, Sun J, Wang K, Cai J, Jiang W, Yang P, Wei F, Qu X
International journal of molecular medicine39(5)1285-1290(2017 May)

Kif4 Is Essential for Mouse Oocyte Meiosis.
Camlin NJ, McLaughlin EA, Holt JE
PloS one12(1)e0170650(2017)

Kif4 interacts with EB1 and stabilizes microtubules downstream of Rho-mDia in migrating fibroblasts.
Morris EJ, Nader GP, Ramalingam N, Bartolini F, Gundersen GG
PloS one9(3)e91568(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the silencing of Kif4 regulated VRGFR1 mRNA transcription and sVEGFR1 production in monocytes via the PI3K/Akt signaling pathway, which may be one of the mechanisms responsible for these processes.
Title: Kif4 regulates the expression of VEGFR1 through the PI3K/Akt signaling pathway in RAW264.7 monocytes/macrophages.

Further investigation of the phosphoregulation of meiotic Kif4 revealed that Aurora Kinase and Cdk activity is critical for Kif4 kinetochore localization and interaction with Ndc80 and CENP-C. Finally, Kif4 protein but not gene expression was found to be upregulated with age, suggesting a role for this protein in the decline of oocyte quality with age
Title: Kif4 Is Essential for Mouse Oocyte Meiosis.

Kif4 is necessary for efficient migration of cells.
Title: Kif4 interacts with EB1 and stabilizes microtubules downstream of Rho-mDia in migrating fibroblasts.

Closure of the backdoor by ATP binding and hydrolysis stabilizes two mobile regions, switch I and switch II, to generate the phosphate tube from which hydrolyzed phosphate is released.
Title: Structural basis for the ATP-induced isomerization of kinesin.

KIF4 is involved in the anterograde trafficking of ribosomal constituents to axons
Title: KIF4 mediates anterograde translocation and positioning of ribosomal constituents to axons.

The following Kif4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kif4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68084	XM_011247528.3 Latest version!	Mus musculus kinesin family member 4 (Kif4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu83081	XM_017318397.2 Latest version!	Mus musculus kinesin family member 4 (Kif4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu01939	NM_008446.2 Latest version!	Mus musculus kinesin family member 4 (Kif4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OMu56167	XM_006527855.4 Latest version!	Mus musculus kinesin family member 4 (Kif4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OMu56167	XM_006527855.3	Mus musculus kinesin family member 4 (Kif4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu83081	XM_017318397.1	Mus musculus kinesin family member 4 (Kif4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OMu68084	XM_011247528.2	Mus musculus kinesin family member 4 (Kif4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kif4 ( XM_011247528.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245830.1 Mus musculus kinesin family member 4 (Kif4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu68084
Clone ID Related Accession (Same CDS sequence)	XM_011247528.2 , XM_011247528.3
Accession Version	XM_011247528.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromosome-associated kinesin KIF4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA ATCAGTCTCT GGTGGAAGAG AATGAAAAAT TAAGTCGTGG TCTGAGTGAG 
GCAGCTGGTC AAACAGCCCA GATGCTGGAG AGGATCATTC TGACAGAGCA AGCAAATGAG 
AAAATGAATG CCAAGCTAGA AGAACTCAGG CGGCATGCGG CCTGTAAAGT GGATCTTCAA 
AAGCTCGTGG AGACATTGGA AGACCAGGAG TTAAAAGAAA ACATAGAGAT AATTTGCAAC 
CTACAACAAG TGATTGCCCA GCTATCTGAT GAAGCTGCTG CTTGCATGAC TGCAACCATT 
GATACTGCAG GGGAAGCAGA CACTCAAGTG CAAAGCAGCC CAGATACCAG CAGGTCTTCT 
GATGTTTTCA GCACTCAGCA TGCCCTACGC CAAGCTCAGA TGTCCAAGGA GCTGATTGAG 
TTGAATAAAG CACTTGCACT GAAGGAAGCC CTAGCTAAGA AGATGACTCA GAATGACAAC 
CAACTCCAGC CCATTCAGTT CCAGTACCAG GATAATATTA AAAATCTAGA ATCAGAAGTC 
CTCAGTCTAC AGAGGGAAAA AGAAGAACTG GTTCTTGAAC TTCAAACAGC AAAGAAGGAT 
GCCAACCAAG CCAAGTTGAG TGAGCGCCGT CGTAAACGTC TTCAGGAACT GGAGGGTCAA 
ATAGCTGATT TGAAAAAGAA ACTGCAAGAA CAGTCCAAAC TTCTGAAGCT GAAGGAATCC 
ACAGAGCATA CTGTCTCCAA GCTGAACCAG GAGATTCGGA TGATGAAAAA CCAGCGAGTC 
CAGTTAATGC GTCAAATGAA AGAGGATGCT GAGAAGTTTA GGCAATGGAA GCAGCAAAAA 
GACAAAGAAG TAATCCAGCT AAAAGAACGG GATCGTAAAA GGCAATATGA GCTGCTCAAA 
CTTGAAAGAA ACTTTCAGAA GCAGTCTAAT GTGCTCAGAC GTAAAACTGA GGAGGCAGCA 
GCTGCCAACA AACGTCTTAA GGATGCTCTG CAAAAGCAAA AGGAGGTTGC AGAAAAACGC 
AAAGAGACTC AGAGCCGTGG GATGGAAAGC ACTGCAGCTA GAATGAAGAA TTGGCTTGGA 
AATGAAATTG AGGTTATGGT CAGTACTGAG GAAGCCAAGC GACATCTGAA TGGCCTTCTT 
GAAGAGAGAA AGATCCTGGC TCAGGATGTG GCACAACTCA AAGAGAAAAG AGAATCTGGA 
GAGAACCCAC CTCTTAAACT CCGAAGGCGC ACATTTTCCT ACGATGAAAT ACATGGTCAA 
GATTCAGGAG CAGAGGATTC TATTGCCAAG CAGATTGAAA GCCTAGAGAC CGAATTGGAG 
CTCAGGAGTG CGCAGATTGC GGACCTACAG CAAAAGCTGC TGGATGCTGA AAGTGAAGAC 
CGGCCGAAAC AGCGCTGGGA GAGTATCGCC ACAATTCTCG AAGCCAAATG TGCCATAAAG 
TATTTAGTGG GAGAGCTCGT CTCCTCCAAA ATACTGGTGA GCAAACTAGA AAGCAGCCTG 
AATCAGAGCA AGGCCAGCTG TATTGACGTG CAGAAGATGC TGTTTGAGGA GCAAAATCAT 
TTTGCTAAGA TAGAGACAGA GTTAAAAGAA GAGCTGGTCA AAGTGGAGCA ACAGCACCAA 
GAAAAGGTGT TGTACCTTCT CAGCCAGCTG CAGCAATCCC AGATGACAGA GAAGCAGCTG 
GAGGAGTCAG TTAGTGAAAA GGAACAGCAG CTGCTGAGCA CACTAAAATG TCAGGAGGAA 
GAGCTTAGGA AGATGCAAGA AGTGTGTGAG CAAAATCAGC AGCTTCTCCA AGAAAACAGT 
GCCATCAAGC AGAAACTGAC CCTACTCCAA GTAGCCAGCA AACAGAAACC CCATCTCACA 
AGAAATATCT TCCAGTCTCC AGACTCTTCC TTTGAATATA TTCCACCTAA GCCCAAACCT 
TGCCGCATTA AGGAAAAATG CCTAGAGCAA AGCTTCGCCG TCGAGGGGCT GCAGTATTAT 
TCAGAGCCTT CTGTGGCCGA GCAGGACAAT GAGGACAGTG ACGACCATGC TGATGAGGAG 
TGGATCCCAA CAAAACTAGT CAAGGTGTCC AAGAAGAGCA TCCAAGGGTG TTCCTGCAAA 
GGTTGGTGCG GGAACAAGCA ATGTGGATGC AGAAAGCAAA AGTCAGACTG TAATGTGTCC 
TGCAGCTGTG ACCCCACAAA GTGTCGCAAC CGCCATCAGA ACCAGGACAA TTCAGATGCT 
ATTGAGCTAA ACCAAGATTC TGAAAACTCT TTCAAACTGG AAGATCCCAC AGAGGTGACC 
TCAGGCTTAA GCTTCTTCCA CCCTATCTGT GCAACTCCCA GTAGCAAGAT CCTAAAAGAA 
ATGTGTGATG CAGATCAGGT GCAGCTGAAG CAGCCCGTGT TTGTCTCTTC TTCTGACCAC 
CCAGAACTGA AGTCTATAGC GTCAGAATCA CAAGAAAATA AGGCCATAGG GAAGAAGAAG 
AAGCGAGCTC TGGCCAGCAA TACCAGCTTC TTCTCTGGCT GCTCTCCTAT CCAAGAAGAG 
TCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245830.1
CDS	1695..4223
Translation	MEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANEKMNAKLEELRRHAACKVDLQKLVETLEDQELKENIEIICNLQQVIAQLSDEAAACMTATIDTAGEADTQVQSSPDTSRSSDVFSTQHALRQAQMSKELIELNKALALKEALAKKMTQNDNQLQPIQFQYQDNIKNLESEVLSLQREKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLQEQSKLLKLKESTEHTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQQKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQKEVAEKRKETQSRGMESTAARMKNWLGNEIEVMVSTEEAKRHLNGLLEERKILAQDVAQLKEKRESGENPPLKLRRRTFSYDEIHGQDSGAEDSIAKQIESLETELELRSAQIADLQQKLLDAESEDRPKQRWESIATILEAKCAIKYLVGELVSSKILVSKLESSLNQSKASCIDVQKMLFEEQNHFAKIETELKEELVKVEQQHQEKVLYLLSQLQQSQMTEKQLEESVSEKEQQLLSTLKCQEEELRKMQEVCEQNQQLLQENSAIKQKLTLLQVASKQKPHLTRNIFQSPDSSFEYIPPKPKPCRIKEKCLEQSFAVEGLQYYSEPSVAEQDNEDSDDHADEEWIPTKLVKVSKKSIQGCSCKGWCGNKQCGCRKQKSDCNVSCSCDPTKCRNRHQNQDNSDAIELNQDSENSFKLEDPTEVTSGLSFFHPICATPSSKILKEMCDADQVQLKQPVFVSSSDHPELKSIASESQENKAIGKKKKRALASNTSFFSGCSPIQEESH

Target ORF information:

RefSeq Version	XM_011247528.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 4 (Kif4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247528.2

ORF Insert Sequence:

ATGGAGAAGA ATCAGTCTCT GGTGGAAGAG AATGAAAAAT TAAGTCGTGG TCTGAGTGAG 
GCAGCTGGTC AAACAGCCCA GATGCTGGAG AGGATCATTC TGACAGAGCA AGCAAATGAG 
AAAATGAATG CCAAGCTAGA AGAACTCAGG CGGCATGCGG CCTGTAAAGT GGATCTTCAA 
AAGCTCGTGG AGACATTGGA AGACCAGGAG TTAAAAGAAA ACATAGAGAT AATTTGCAAC 
CTACAACAAG TGATTGCCCA GCTATCTGAT GAAGCTGCTG CTTGCATGAC TGCAACCATT 
GATACTGCAG GGGAAGCAGA CACTCAAGTG CAAAGCAGCC CAGATACCAG CAGGTCTTCT 
GATGTTTTCA GCACTCAGCA TGCCCTACGC CAAGCTCAGA TGTCCAAGGA GCTGATTGAG 
TTGAATAAAG CACTTGCACT GAAGGAAGCC CTAGCTAAGA AGATGACTCA GAATGACAAC 
CAACTCCAGC CCATTCAGTT CCAGTACCAG GATAATATTA AAAATCTAGA ATCAGAAGTC 
CTCAGTCTAC AGAGGGAAAA AGAAGAACTG GTTCTTGAAC TTCAAACAGC AAAGAAGGAT 
GCCAACCAAG CCAAGTTGAG TGAGCGCCGT CGTAAACGTC TTCAGGAACT GGAGGGTCAA 
ATAGCTGATT TGAAAAAGAA ACTGCAAGAA CAGTCCAAAC TTCTGAAGCT GAAGGAATCC 
ACAGAGCATA CTGTCTCCAA GCTGAACCAG GAGATTCGGA TGATGAAAAA CCAGCGAGTC 
CAGTTAATGC GTCAAATGAA AGAGGATGCT GAGAAGTTTA GGCAATGGAA GCAGCAAAAA 
GACAAAGAAG TAATCCAGCT AAAAGAACGG GATCGTAAAA GGCAATATGA GCTGCTCAAA 
CTTGAAAGAA ACTTTCAGAA GCAGTCTAAT GTGCTCAGAC GTAAAACTGA GGAGGCAGCA 
GCTGCCAACA AACGTCTTAA GGATGCTCTG CAAAAGCAAA AGGAGGTTGC AGAAAAACGC 
AAAGAGACTC AGAGCCGTGG GATGGAAAGC ACTGCAGCTA GAATGAAGAA TTGGCTTGGA 
AATGAAATTG AGGTTATGGT CAGTACTGAG GAAGCCAAGC GACATCTGAA TGGCCTTCTT 
GAAGAGAGAA AGATCCTGGC TCAGGATGTG GCACAACTCA AAGAGAAAAG AGAATCTGGA 
GAGAACCCAC CTCTTAAACT CCGAAGGCGC ACATTTTCCT ACGATGAAAT ACATGGTCAA 
GATTCAGGAG CAGAGGATTC TATTGCCAAG CAGATTGAAA GCCTAGAGAC CGAATTGGAG 
CTCAGGAGTG CGCAGATTGC GGACCTACAG CAAAAGCTGC TGGATGCTGA AAGTGAAGAC 
CGGCCGAAAC AGCGCTGGGA GAGTATCGCC ACAATTCTCG AAGCCAAATG TGCCATAAAG 
TATTTAGTGG GAGAGCTCGT CTCCTCCAAA ATACTGGTGA GCAAACTAGA AAGCAGCCTG 
AATCAGAGCA AGGCCAGCTG TATTGACGTG CAGAAGATGC TGTTTGAGGA GCAAAATCAT 
TTTGCTAAGA TAGAGACAGA GTTAAAAGAA GAGCTGGTCA AAGTGGAGCA ACAGCACCAA 
GAAAAGGTGT TGTACCTTCT CAGCCAGCTG CAGCAATCCC AGATGACAGA GAAGCAGCTG 
GAGGAGTCAG TTAGTGAAAA GGAACAGCAG CTGCTGAGCA CACTAAAATG TCAGGAGGAA 
GAGCTTAGGA AGATGCAAGA AGTGTGTGAG CAAAATCAGC AGCTTCTCCA AGAAAACAGT 
GCCATCAAGC AGAAACTGAC CCTACTCCAA GTAGCCAGCA AACAGAAACC CCATCTCACA 
AGAAATATCT TCCAGTCTCC AGACTCTTCC TTTGAATATA TTCCACCTAA GCCCAAACCT 
TGCCGCATTA AGGAAAAATG CCTAGAGCAA AGCTTCGCCG TCGAGGGGCT GCAGTATTAT 
TCAGAGCCTT CTGTGGCCGA GCAGGACAAT GAGGACAGTG ACGACCATGC TGATGAGGAG 
TGGATCCCAA CAAAACTAGT CAAGGTGTCC AAGAAGAGCA TCCAAGGGTG TTCCTGCAAA 
GGTTGGTGCG GGAACAAGCA ATGTGGATGC AGAAAGCAAA AGTCAGACTG TAATGTGTCC 
TGCAGCTGTG ACCCCACAAA GTGTCGCAAC CGCCATCAGA ACCAGGACAA TTCAGATGCT 
ATTGAGCTAA ACCAAGATTC TGAAAACTCT TTCAAACTGG AAGATCCCAC AGAGGTGACC 
TCAGGCTTAA GCTTCTTCCA CCCTATCTGT GCAACTCCCA GTAGCAAGAT CCTAAAAGAA 
ATGTGTGATG CAGATCAGGT GCAGCTGAAG CAGCCCGTGT TTGTCTCTTC TTCTGACCAC 
CCAGAACTGA AGTCTATAGC GTCAGAATCA CAAGAAAATA AGGCCATAGG GAAGAAGAAG 
AAGCGAGCTC TGGCCAGCAA TACCAGCTTC TTCTCTGGCT GCTCTCCTAT CCAAGAAGAG 
TCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif4 ( XM_017318397.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173886.1 Mus musculus kinesin family member 4 (Kif4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu83081
Clone ID Related Accession (Same CDS sequence)	XM_017318397.1 , XM_017318397.2
Accession Version	XM_017318397.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromosome-associated kinesin KIF4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CTGCTGCTTG CATGACTGCA ACCATTGATA CTGCAGGGGA AGCAGACACT 
CAAGTGCAAA GCAGCCCAGA TACCAGCAGG TCTTCTGATG TTTTCAGCAC TCAGCATGCC 
CTACGCCAAG CTCAGATGTC CAAGGAGCTG ATTGAGTTGA ATAAAGCACT TGCACTGAAG 
GAAGCCCTAG CTAAGAAGAT GACTCAGAAT GACAACCAAC TCCAGCCCAT TCAGTTCCAG 
TACCAGGATA ATATTAAAAA TCTAGAATCA GAAGTCCTCA GTCTACAGAG GGAAAAAGAA 
GAACTGGTTC TTGAACTTCA AACAGCAAAG AAGGATGCCA ACCAAGCCAA GTTGAGTGAG 
CGCCGTCGTA AACGTCTTCA GGAACTGGAG GGTCAAATAG CTGATTTGAA AAAGAAACTG 
CAAGAACAGT CCAAACTTCT GAAGCTGAAG GAATCCACAG AGCATACTGT CTCCAAGCTG 
AACCAGGAGA TTCGGATGAT GAAAAACCAG CGAGTCCAGT TAATGCGTCA AATGAAAGAG 
GATGCTGAGA AGTTTAGGCA ATGGAAGCAG CAAAAAGACA AAGAAGTAAT CCAGCTAAAA 
GAACGGGATC GTAAAAGGCA ATATGAGCTG CTCAAACTTG AAAGAAACTT TCAGAAGCAG 
TCTAATGTGC TCAGACGTAA AACTGAGGAG GCAGCAGCTG CCAACAAACG TCTTAAGGAT 
GCTCTGCAAA AGCAAAAGGA GGTTGCAGAA AAACGCAAAG AGACTCAGAG CCGTGGGATG 
GAAAGCACTG CAGCTAGAAT GAAGAATTGG CTTGGAAATG AAATTGAGGT TATGGTCAGT 
ACTGAGGAAG CCAAGCGACA TCTGAATGGC CTTCTTGAAG AGAGAAAGAT CCTGGCTCAG 
GATGTGGCAC AACTCAAAGA GAAAAGAGAA TCTGGAGAGA ACCCACCTCT TAAACTCCGA 
AGGCGCACAT TTTCCTACGA TGAAATACAT GGTCAAGATT CAGGAGCAGA GGATTCTATT 
GCCAAGCAGA TTGAAAGCCT AGAGACCGAA TTGGAGCTCA GGAGTGCGCA GATTGCGGAC 
CTACAGCAAA AGCTGCTGGA TGCTGAAAGT GAAGACCGGC CGAAACAGCG CTGGGAGAGT 
ATCGCCACAA TTCTCGAAGC CAAATGTGCC ATAAAGTATT TAGTGGGAGA GCTCGTCTCC 
TCCAAAATAC TGGTGAGCAA ACTAGAAAGC AGCCTGAATC AGAGCAAGGC CAGCTGTATT 
GACGTGCAGA AGATGCTGTT TGAGGAGCAA AATCATTTTG CTAAGATAGA GACAGAGTTA 
AAAGAAGAGC TGGTCAAAGT GGAGCAACAG CACCAAGAAA AGGTGTTGTA CCTTCTCAGC 
CAGCTGCAGC AATCCCAGAT GACAGAGAAG CAGCTGGAGG AGTCAGTTAG TGAAAAGGAA 
CAGCAGCTGC TGAGCACACT AAAATGTCAG GAGGAAGAGC TTAGGAAGAT GCAAGAAGTG 
TGTGAGCAAA ATCAGCAGCT TCTCCAAGAA AACAGTGCCA TCAAGCAGAA ACTGACCCTA 
CTCCAAGTAG CCAGCAAACA GAAACCCCAT CTCACAAGAA ATATCTTCCA GTCTCCAGAC 
TCTTCCTTTG AATATATTCC ACCTAAGCCC AAACCTTGCC GCATTAAGGA AAAATGCCTA 
GAGCAAAGCT TCGCCGTCGA GGGGCTGCAG TATTATTCAG AGCCTTCTGT GGCCGAGCAG 
GACAATGAGG ACAGTGACGA CCATGCTGAT GAGGAGTGGA TCCCAACAAA ACTAGTCAAG 
GTGTCCAAGA AGAGCATCCA AGGGTGTTCC TGCAAAGGTT GGTGCGGGAA CAAGCAATGT 
GGATGCAGAA AGCAAAAGTC AGACTGTAAT GTGTCCTGCA GCTGTGACCC CACAAAGTGT 
CGCAACCGCC ATCAGAACCA GGACAATTCA GATGCTATTG AGCTAAACCA AGATTCTGAA 
AACTCTTTCA AACTGGAAGA TCCCACAGAG GTGACCTCAG GCTTAAGCTT CTTCCACCCT 
ATCTGTGCAA CTCCCAGTAG CAAGATCCTA AAAGAAATGT GTGATGCAGA TCAGGTGCAG 
CTGAAGCAGC CCGTGTTTGT CTCTTCTTCT GACCACCCAG AACTGAAGTC TATAGCGTCA 
GAATCACAAG AAAATAAGGC CATAGGGAAG AAGAAGAAGC GAGCTCTGGC CAGCAATACC 
AGCTTCTTCT CTGGCTGCTC TCCTATCCAA GAAGAGTCCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173886.1
CDS	242..2506
Translation	MDEAAACMTATIDTAGEADTQVQSSPDTSRSSDVFSTQHALRQAQMSKELIELNKALALKEALAKKMTQNDNQLQPIQFQYQDNIKNLESEVLSLQREKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLQEQSKLLKLKESTEHTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQQKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQKEVAEKRKETQSRGMESTAARMKNWLGNEIEVMVSTEEAKRHLNGLLEERKILAQDVAQLKEKRESGENPPLKLRRRTFSYDEIHGQDSGAEDSIAKQIESLETELELRSAQIADLQQKLLDAESEDRPKQRWESIATILEAKCAIKYLVGELVSSKILVSKLESSLNQSKASCIDVQKMLFEEQNHFAKIETELKEELVKVEQQHQEKVLYLLSQLQQSQMTEKQLEESVSEKEQQLLSTLKCQEEELRKMQEVCEQNQQLLQENSAIKQKLTLLQVASKQKPHLTRNIFQSPDSSFEYIPPKPKPCRIKEKCLEQSFAVEGLQYYSEPSVAEQDNEDSDDHADEEWIPTKLVKVSKKSIQGCSCKGWCGNKQCGCRKQKSDCNVSCSCDPTKCRNRHQNQDNSDAIELNQDSENSFKLEDPTEVTSGLSFFHPICATPSSKILKEMCDADQVQLKQPVFVSSSDHPELKSIASESQENKAIGKKKKRALASNTSFFSGCSPIQEESH

Target ORF information:

RefSeq Version	XM_017318397.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 4 (Kif4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318397.1

ORF Insert Sequence:

ATGGATGAAG CTGCTGCTTG CATGACTGCA ACCATTGATA CTGCAGGGGA AGCAGACACT 
CAAGTGCAAA GCAGCCCAGA TACCAGCAGG TCTTCTGATG TTTTCAGCAC TCAGCATGCC 
CTACGCCAAG CTCAGATGTC CAAGGAGCTG ATTGAGTTGA ATAAAGCACT TGCACTGAAG 
GAAGCCCTAG CTAAGAAGAT GACTCAGAAT GACAACCAAC TCCAGCCCAT TCAGTTCCAG 
TACCAGGATA ATATTAAAAA TCTAGAATCA GAAGTCCTCA GTCTACAGAG GGAAAAAGAA 
GAACTGGTTC TTGAACTTCA AACAGCAAAG AAGGATGCCA ACCAAGCCAA GTTGAGTGAG 
CGCCGTCGTA AACGTCTTCA GGAACTGGAG GGTCAAATAG CTGATTTGAA AAAGAAACTG 
CAAGAACAGT CCAAACTTCT GAAGCTGAAG GAATCCACAG AGCATACTGT CTCCAAGCTG 
AACCAGGAGA TTCGGATGAT GAAAAACCAG CGAGTCCAGT TAATGCGTCA AATGAAAGAG 
GATGCTGAGA AGTTTAGGCA ATGGAAGCAG CAAAAAGACA AAGAAGTAAT CCAGCTAAAA 
GAACGGGATC GTAAAAGGCA ATATGAGCTG CTCAAACTTG AAAGAAACTT TCAGAAGCAG 
TCTAATGTGC TCAGACGTAA AACTGAGGAG GCAGCAGCTG CCAACAAACG TCTTAAGGAT 
GCTCTGCAAA AGCAAAAGGA GGTTGCAGAA AAACGCAAAG AGACTCAGAG CCGTGGGATG 
GAAAGCACTG CAGCTAGAAT GAAGAATTGG CTTGGAAATG AAATTGAGGT TATGGTCAGT 
ACTGAGGAAG CCAAGCGACA TCTGAATGGC CTTCTTGAAG AGAGAAAGAT CCTGGCTCAG 
GATGTGGCAC AACTCAAAGA GAAAAGAGAA TCTGGAGAGA ACCCACCTCT TAAACTCCGA 
AGGCGCACAT TTTCCTACGA TGAAATACAT GGTCAAGATT CAGGAGCAGA GGATTCTATT 
GCCAAGCAGA TTGAAAGCCT AGAGACCGAA TTGGAGCTCA GGAGTGCGCA GATTGCGGAC 
CTACAGCAAA AGCTGCTGGA TGCTGAAAGT GAAGACCGGC CGAAACAGCG CTGGGAGAGT 
ATCGCCACAA TTCTCGAAGC CAAATGTGCC ATAAAGTATT TAGTGGGAGA GCTCGTCTCC 
TCCAAAATAC TGGTGAGCAA ACTAGAAAGC AGCCTGAATC AGAGCAAGGC CAGCTGTATT 
GACGTGCAGA AGATGCTGTT TGAGGAGCAA AATCATTTTG CTAAGATAGA GACAGAGTTA 
AAAGAAGAGC TGGTCAAAGT GGAGCAACAG CACCAAGAAA AGGTGTTGTA CCTTCTCAGC 
CAGCTGCAGC AATCCCAGAT GACAGAGAAG CAGCTGGAGG AGTCAGTTAG TGAAAAGGAA 
CAGCAGCTGC TGAGCACACT AAAATGTCAG GAGGAAGAGC TTAGGAAGAT GCAAGAAGTG 
TGTGAGCAAA ATCAGCAGCT TCTCCAAGAA AACAGTGCCA TCAAGCAGAA ACTGACCCTA 
CTCCAAGTAG CCAGCAAACA GAAACCCCAT CTCACAAGAA ATATCTTCCA GTCTCCAGAC 
TCTTCCTTTG AATATATTCC ACCTAAGCCC AAACCTTGCC GCATTAAGGA AAAATGCCTA 
GAGCAAAGCT TCGCCGTCGA GGGGCTGCAG TATTATTCAG AGCCTTCTGT GGCCGAGCAG 
GACAATGAGG ACAGTGACGA CCATGCTGAT GAGGAGTGGA TCCCAACAAA ACTAGTCAAG 
GTGTCCAAGA AGAGCATCCA AGGGTGTTCC TGCAAAGGTT GGTGCGGGAA CAAGCAATGT 
GGATGCAGAA AGCAAAAGTC AGACTGTAAT GTGTCCTGCA GCTGTGACCC CACAAAGTGT 
CGCAACCGCC ATCAGAACCA GGACAATTCA GATGCTATTG AGCTAAACCA AGATTCTGAA 
AACTCTTTCA AACTGGAAGA TCCCACAGAG GTGACCTCAG GCTTAAGCTT CTTCCACCCT 
ATCTGTGCAA CTCCCAGTAG CAAGATCCTA AAAGAAATGT GTGATGCAGA TCAGGTGCAG 
CTGAAGCAGC CCGTGTTTGT CTCTTCTTCT GACCACCCAG AACTGAAGTC TATAGCGTCA 
GAATCACAAG AAAATAAGGC CATAGGGAAG AAGAAGAAGC GAGCTCTGGC CAGCAATACC 
AGCTTCTTCT CTGGCTGCTC TCCTATCCAA GAAGAGTCCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif4 ( XM_011247528.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245830.1 Mus musculus kinesin family member 4 (Kif4), transcript variant X2, mRNA.

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CloneID	OMu68084
Clone ID Related Accession (Same CDS sequence)	XM_011247528.2 , XM_011247528.3
Accession Version	XM_011247528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromosome-associated kinesin KIF4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA ATCAGTCTCT GGTGGAAGAG AATGAAAAAT TAAGTCGTGG TCTGAGTGAG 
GCAGCTGGTC AAACAGCCCA GATGCTGGAG AGGATCATTC TGACAGAGCA AGCAAATGAG 
AAAATGAATG CCAAGCTAGA AGAACTCAGG CGGCATGCGG CCTGTAAAGT GGATCTTCAA 
AAGCTCGTGG AGACATTGGA AGACCAGGAG TTAAAAGAAA ACATAGAGAT AATTTGCAAC 
CTACAACAAG TGATTGCCCA GCTATCTGAT GAAGCTGCTG CTTGCATGAC TGCAACCATT 
GATACTGCAG GGGAAGCAGA CACTCAAGTG CAAAGCAGCC CAGATACCAG CAGGTCTTCT 
GATGTTTTCA GCACTCAGCA TGCCCTACGC CAAGCTCAGA TGTCCAAGGA GCTGATTGAG 
TTGAATAAAG CACTTGCACT GAAGGAAGCC CTAGCTAAGA AGATGACTCA GAATGACAAC 
CAACTCCAGC CCATTCAGTT CCAGTACCAG GATAATATTA AAAATCTAGA ATCAGAAGTC 
CTCAGTCTAC AGAGGGAAAA AGAAGAACTG GTTCTTGAAC TTCAAACAGC AAAGAAGGAT 
GCCAACCAAG CCAAGTTGAG TGAGCGCCGT CGTAAACGTC TTCAGGAACT GGAGGGTCAA 
ATAGCTGATT TGAAAAAGAA ACTGCAAGAA CAGTCCAAAC TTCTGAAGCT GAAGGAATCC 
ACAGAGCATA CTGTCTCCAA GCTGAACCAG GAGATTCGGA TGATGAAAAA CCAGCGAGTC 
CAGTTAATGC GTCAAATGAA AGAGGATGCT GAGAAGTTTA GGCAATGGAA GCAGCAAAAA 
GACAAAGAAG TAATCCAGCT AAAAGAACGG GATCGTAAAA GGCAATATGA GCTGCTCAAA 
CTTGAAAGAA ACTTTCAGAA GCAGTCTAAT GTGCTCAGAC GTAAAACTGA GGAGGCAGCA 
GCTGCCAACA AACGTCTTAA GGATGCTCTG CAAAAGCAAA AGGAGGTTGC AGAAAAACGC 
AAAGAGACTC AGAGCCGTGG GATGGAAAGC ACTGCAGCTA GAATGAAGAA TTGGCTTGGA 
AATGAAATTG AGGTTATGGT CAGTACTGAG GAAGCCAAGC GACATCTGAA TGGCCTTCTT 
GAAGAGAGAA AGATCCTGGC TCAGGATGTG GCACAACTCA AAGAGAAAAG AGAATCTGGA 
GAGAACCCAC CTCTTAAACT CCGAAGGCGC ACATTTTCCT ACGATGAAAT ACATGGTCAA 
GATTCAGGAG CAGAGGATTC TATTGCCAAG CAGATTGAAA GCCTAGAGAC CGAATTGGAG 
CTCAGGAGTG CGCAGATTGC GGACCTACAG CAAAAGCTGC TGGATGCTGA AAGTGAAGAC 
CGGCCGAAAC AGCGCTGGGA GAGTATCGCC ACAATTCTCG AAGCCAAATG TGCCATAAAG 
TATTTAGTGG GAGAGCTCGT CTCCTCCAAA ATACTGGTGA GCAAACTAGA AAGCAGCCTG 
AATCAGAGCA AGGCCAGCTG TATTGACGTG CAGAAGATGC TGTTTGAGGA GCAAAATCAT 
TTTGCTAAGA TAGAGACAGA GTTAAAAGAA GAGCTGGTCA AAGTGGAGCA ACAGCACCAA 
GAAAAGGTGT TGTACCTTCT CAGCCAGCTG CAGCAATCCC AGATGACAGA GAAGCAGCTG 
GAGGAGTCAG TTAGTGAAAA GGAACAGCAG CTGCTGAGCA CACTAAAATG TCAGGAGGAA 
GAGCTTAGGA AGATGCAAGA AGTGTGTGAG CAAAATCAGC AGCTTCTCCA AGAAAACAGT 
GCCATCAAGC AGAAACTGAC CCTACTCCAA GTAGCCAGCA AACAGAAACC CCATCTCACA 
AGAAATATCT TCCAGTCTCC AGACTCTTCC TTTGAATATA TTCCACCTAA GCCCAAACCT 
TGCCGCATTA AGGAAAAATG CCTAGAGCAA AGCTTCGCCG TCGAGGGGCT GCAGTATTAT 
TCAGAGCCTT CTGTGGCCGA GCAGGACAAT GAGGACAGTG ACGACCATGC TGATGAGGAG 
TGGATCCCAA CAAAACTAGT CAAGGTGTCC AAGAAGAGCA TCCAAGGGTG TTCCTGCAAA 
GGTTGGTGCG GGAACAAGCA ATGTGGATGC AGAAAGCAAA AGTCAGACTG TAATGTGTCC 
TGCAGCTGTG ACCCCACAAA GTGTCGCAAC CGCCATCAGA ACCAGGACAA TTCAGATGCT 
ATTGAGCTAA ACCAAGATTC TGAAAACTCT TTCAAACTGG AAGATCCCAC AGAGGTGACC 
TCAGGCTTAA GCTTCTTCCA CCCTATCTGT GCAACTCCCA GTAGCAAGAT CCTAAAAGAA 
ATGTGTGATG CAGATCAGGT GCAGCTGAAG CAGCCCGTGT TTGTCTCTTC TTCTGACCAC 
CCAGAACTGA AGTCTATAGC GTCAGAATCA CAAGAAAATA AGGCCATAGG GAAGAAGAAG 
AAGCGAGCTC TGGCCAGCAA TACCAGCTTC TTCTCTGGCT GCTCTCCTAT CCAAGAAGAG 
TCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245830.1
CDS	1696..4224
Translation	MEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANEKMNAKLEELRRHAACKVDLQKLVETLEDQELKENIEIICNLQQVIAQLSDEAAACMTATIDTAGEADTQVQSSPDTSRSSDVFSTQHALRQAQMSKELIELNKALALKEALAKKMTQNDNQLQPIQFQYQDNIKNLESEVLSLQREKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLQEQSKLLKLKESTEHTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQQKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQKEVAEKRKETQSRGMESTAARMKNWLGNEIEVMVSTEEAKRHLNGLLEERKILAQDVAQLKEKRESGENPPLKLRRRTFSYDEIHGQDSGAEDSIAKQIESLETELELRSAQIADLQQKLLDAESEDRPKQRWESIATILEAKCAIKYLVGELVSSKILVSKLESSLNQSKASCIDVQKMLFEEQNHFAKIETELKEELVKVEQQHQEKVLYLLSQLQQSQMTEKQLEESVSEKEQQLLSTLKCQEEELRKMQEVCEQNQQLLQENSAIKQKLTLLQVASKQKPHLTRNIFQSPDSSFEYIPPKPKPCRIKEKCLEQSFAVEGLQYYSEPSVAEQDNEDSDDHADEEWIPTKLVKVSKKSIQGCSCKGWCGNKQCGCRKQKSDCNVSCSCDPTKCRNRHQNQDNSDAIELNQDSENSFKLEDPTEVTSGLSFFHPICATPSSKILKEMCDADQVQLKQPVFVSSSDHPELKSIASESQENKAIGKKKKRALASNTSFFSGCSPIQEESH

Target ORF information:

RefSeq Version	XM_011247528.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 4 (Kif4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247528.3

ORF Insert Sequence:

ATGGAGAAGA ATCAGTCTCT GGTGGAAGAG AATGAAAAAT TAAGTCGTGG TCTGAGTGAG 
GCAGCTGGTC AAACAGCCCA GATGCTGGAG AGGATCATTC TGACAGAGCA AGCAAATGAG 
AAAATGAATG CCAAGCTAGA AGAACTCAGG CGGCATGCGG CCTGTAAAGT GGATCTTCAA 
AAGCTCGTGG AGACATTGGA AGACCAGGAG TTAAAAGAAA ACATAGAGAT AATTTGCAAC 
CTACAACAAG TGATTGCCCA GCTATCTGAT GAAGCTGCTG CTTGCATGAC TGCAACCATT 
GATACTGCAG GGGAAGCAGA CACTCAAGTG CAAAGCAGCC CAGATACCAG CAGGTCTTCT 
GATGTTTTCA GCACTCAGCA TGCCCTACGC CAAGCTCAGA TGTCCAAGGA GCTGATTGAG 
TTGAATAAAG CACTTGCACT GAAGGAAGCC CTAGCTAAGA AGATGACTCA GAATGACAAC 
CAACTCCAGC CCATTCAGTT CCAGTACCAG GATAATATTA AAAATCTAGA ATCAGAAGTC 
CTCAGTCTAC AGAGGGAAAA AGAAGAACTG GTTCTTGAAC TTCAAACAGC AAAGAAGGAT 
GCCAACCAAG CCAAGTTGAG TGAGCGCCGT CGTAAACGTC TTCAGGAACT GGAGGGTCAA 
ATAGCTGATT TGAAAAAGAA ACTGCAAGAA CAGTCCAAAC TTCTGAAGCT GAAGGAATCC 
ACAGAGCATA CTGTCTCCAA GCTGAACCAG GAGATTCGGA TGATGAAAAA CCAGCGAGTC 
CAGTTAATGC GTCAAATGAA AGAGGATGCT GAGAAGTTTA GGCAATGGAA GCAGCAAAAA 
GACAAAGAAG TAATCCAGCT AAAAGAACGG GATCGTAAAA GGCAATATGA GCTGCTCAAA 
CTTGAAAGAA ACTTTCAGAA GCAGTCTAAT GTGCTCAGAC GTAAAACTGA GGAGGCAGCA 
GCTGCCAACA AACGTCTTAA GGATGCTCTG CAAAAGCAAA AGGAGGTTGC AGAAAAACGC 
AAAGAGACTC AGAGCCGTGG GATGGAAAGC ACTGCAGCTA GAATGAAGAA TTGGCTTGGA 
AATGAAATTG AGGTTATGGT CAGTACTGAG GAAGCCAAGC GACATCTGAA TGGCCTTCTT 
GAAGAGAGAA AGATCCTGGC TCAGGATGTG GCACAACTCA AAGAGAAAAG AGAATCTGGA 
GAGAACCCAC CTCTTAAACT CCGAAGGCGC ACATTTTCCT ACGATGAAAT ACATGGTCAA 
GATTCAGGAG CAGAGGATTC TATTGCCAAG CAGATTGAAA GCCTAGAGAC CGAATTGGAG 
CTCAGGAGTG CGCAGATTGC GGACCTACAG CAAAAGCTGC TGGATGCTGA AAGTGAAGAC 
CGGCCGAAAC AGCGCTGGGA GAGTATCGCC ACAATTCTCG AAGCCAAATG TGCCATAAAG 
TATTTAGTGG GAGAGCTCGT CTCCTCCAAA ATACTGGTGA GCAAACTAGA AAGCAGCCTG 
AATCAGAGCA AGGCCAGCTG TATTGACGTG CAGAAGATGC TGTTTGAGGA GCAAAATCAT 
TTTGCTAAGA TAGAGACAGA GTTAAAAGAA GAGCTGGTCA AAGTGGAGCA ACAGCACCAA 
GAAAAGGTGT TGTACCTTCT CAGCCAGCTG CAGCAATCCC AGATGACAGA GAAGCAGCTG 
GAGGAGTCAG TTAGTGAAAA GGAACAGCAG CTGCTGAGCA CACTAAAATG TCAGGAGGAA 
GAGCTTAGGA AGATGCAAGA AGTGTGTGAG CAAAATCAGC AGCTTCTCCA AGAAAACAGT 
GCCATCAAGC AGAAACTGAC CCTACTCCAA GTAGCCAGCA AACAGAAACC CCATCTCACA 
AGAAATATCT TCCAGTCTCC AGACTCTTCC TTTGAATATA TTCCACCTAA GCCCAAACCT 
TGCCGCATTA AGGAAAAATG CCTAGAGCAA AGCTTCGCCG TCGAGGGGCT GCAGTATTAT 
TCAGAGCCTT CTGTGGCCGA GCAGGACAAT GAGGACAGTG ACGACCATGC TGATGAGGAG 
TGGATCCCAA CAAAACTAGT CAAGGTGTCC AAGAAGAGCA TCCAAGGGTG TTCCTGCAAA 
GGTTGGTGCG GGAACAAGCA ATGTGGATGC AGAAAGCAAA AGTCAGACTG TAATGTGTCC 
TGCAGCTGTG ACCCCACAAA GTGTCGCAAC CGCCATCAGA ACCAGGACAA TTCAGATGCT 
ATTGAGCTAA ACCAAGATTC TGAAAACTCT TTCAAACTGG AAGATCCCAC AGAGGTGACC 
TCAGGCTTAA GCTTCTTCCA CCCTATCTGT GCAACTCCCA GTAGCAAGAT CCTAAAAGAA 
ATGTGTGATG CAGATCAGGT GCAGCTGAAG CAGCCCGTGT TTGTCTCTTC TTCTGACCAC 
CCAGAACTGA AGTCTATAGC GTCAGAATCA CAAGAAAATA AGGCCATAGG GAAGAAGAAG 
AAGCGAGCTC TGGCCAGCAA TACCAGCTTC TTCTCTGGCT GCTCTCCTAT CCAAGAAGAG 
TCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif4 ( XM_017318397.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173886.1 Mus musculus kinesin family member 4 (Kif4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu83081
Clone ID Related Accession (Same CDS sequence)	XM_017318397.1 , XM_017318397.2
Accession Version	XM_017318397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromosome-associated kinesin KIF4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG CTGCTGCTTG CATGACTGCA ACCATTGATA CTGCAGGGGA AGCAGACACT 
CAAGTGCAAA GCAGCCCAGA TACCAGCAGG TCTTCTGATG TTTTCAGCAC TCAGCATGCC 
CTACGCCAAG CTCAGATGTC CAAGGAGCTG ATTGAGTTGA ATAAAGCACT TGCACTGAAG 
GAAGCCCTAG CTAAGAAGAT GACTCAGAAT GACAACCAAC TCCAGCCCAT TCAGTTCCAG 
TACCAGGATA ATATTAAAAA TCTAGAATCA GAAGTCCTCA GTCTACAGAG GGAAAAAGAA 
GAACTGGTTC TTGAACTTCA AACAGCAAAG AAGGATGCCA ACCAAGCCAA GTTGAGTGAG 
CGCCGTCGTA AACGTCTTCA GGAACTGGAG GGTCAAATAG CTGATTTGAA AAAGAAACTG 
CAAGAACAGT CCAAACTTCT GAAGCTGAAG GAATCCACAG AGCATACTGT CTCCAAGCTG 
AACCAGGAGA TTCGGATGAT GAAAAACCAG CGAGTCCAGT TAATGCGTCA AATGAAAGAG 
GATGCTGAGA AGTTTAGGCA ATGGAAGCAG CAAAAAGACA AAGAAGTAAT CCAGCTAAAA 
GAACGGGATC GTAAAAGGCA ATATGAGCTG CTCAAACTTG AAAGAAACTT TCAGAAGCAG 
TCTAATGTGC TCAGACGTAA AACTGAGGAG GCAGCAGCTG CCAACAAACG TCTTAAGGAT 
GCTCTGCAAA AGCAAAAGGA GGTTGCAGAA AAACGCAAAG AGACTCAGAG CCGTGGGATG 
GAAAGCACTG CAGCTAGAAT GAAGAATTGG CTTGGAAATG AAATTGAGGT TATGGTCAGT 
ACTGAGGAAG CCAAGCGACA TCTGAATGGC CTTCTTGAAG AGAGAAAGAT CCTGGCTCAG 
GATGTGGCAC AACTCAAAGA GAAAAGAGAA TCTGGAGAGA ACCCACCTCT TAAACTCCGA 
AGGCGCACAT TTTCCTACGA TGAAATACAT GGTCAAGATT CAGGAGCAGA GGATTCTATT 
GCCAAGCAGA TTGAAAGCCT AGAGACCGAA TTGGAGCTCA GGAGTGCGCA GATTGCGGAC 
CTACAGCAAA AGCTGCTGGA TGCTGAAAGT GAAGACCGGC CGAAACAGCG CTGGGAGAGT 
ATCGCCACAA TTCTCGAAGC CAAATGTGCC ATAAAGTATT TAGTGGGAGA GCTCGTCTCC 
TCCAAAATAC TGGTGAGCAA ACTAGAAAGC AGCCTGAATC AGAGCAAGGC CAGCTGTATT 
GACGTGCAGA AGATGCTGTT TGAGGAGCAA AATCATTTTG CTAAGATAGA GACAGAGTTA 
AAAGAAGAGC TGGTCAAAGT GGAGCAACAG CACCAAGAAA AGGTGTTGTA CCTTCTCAGC 
CAGCTGCAGC AATCCCAGAT GACAGAGAAG CAGCTGGAGG AGTCAGTTAG TGAAAAGGAA 
CAGCAGCTGC TGAGCACACT AAAATGTCAG GAGGAAGAGC TTAGGAAGAT GCAAGAAGTG 
TGTGAGCAAA ATCAGCAGCT TCTCCAAGAA AACAGTGCCA TCAAGCAGAA ACTGACCCTA 
CTCCAAGTAG CCAGCAAACA GAAACCCCAT CTCACAAGAA ATATCTTCCA GTCTCCAGAC 
TCTTCCTTTG AATATATTCC ACCTAAGCCC AAACCTTGCC GCATTAAGGA AAAATGCCTA 
GAGCAAAGCT TCGCCGTCGA GGGGCTGCAG TATTATTCAG AGCCTTCTGT GGCCGAGCAG 
GACAATGAGG ACAGTGACGA CCATGCTGAT GAGGAGTGGA TCCCAACAAA ACTAGTCAAG 
GTGTCCAAGA AGAGCATCCA AGGGTGTTCC TGCAAAGGTT GGTGCGGGAA CAAGCAATGT 
GGATGCAGAA AGCAAAAGTC AGACTGTAAT GTGTCCTGCA GCTGTGACCC CACAAAGTGT 
CGCAACCGCC ATCAGAACCA GGACAATTCA GATGCTATTG AGCTAAACCA AGATTCTGAA 
AACTCTTTCA AACTGGAAGA TCCCACAGAG GTGACCTCAG GCTTAAGCTT CTTCCACCCT 
ATCTGTGCAA CTCCCAGTAG CAAGATCCTA AAAGAAATGT GTGATGCAGA TCAGGTGCAG 
CTGAAGCAGC CCGTGTTTGT CTCTTCTTCT GACCACCCAG AACTGAAGTC TATAGCGTCA 
GAATCACAAG AAAATAAGGC CATAGGGAAG AAGAAGAAGC GAGCTCTGGC CAGCAATACC 
AGCTTCTTCT CTGGCTGCTC TCCTATCCAA GAAGAGTCCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173886.1
CDS	3414..5678
Translation	MDEAAACMTATIDTAGEADTQVQSSPDTSRSSDVFSTQHALRQAQMSKELIELNKALALKEALAKKMTQNDNQLQPIQFQYQDNIKNLESEVLSLQREKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLQEQSKLLKLKESTEHTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQQKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQKEVAEKRKETQSRGMESTAARMKNWLGNEIEVMVSTEEAKRHLNGLLEERKILAQDVAQLKEKRESGENPPLKLRRRTFSYDEIHGQDSGAEDSIAKQIESLETELELRSAQIADLQQKLLDAESEDRPKQRWESIATILEAKCAIKYLVGELVSSKILVSKLESSLNQSKASCIDVQKMLFEEQNHFAKIETELKEELVKVEQQHQEKVLYLLSQLQQSQMTEKQLEESVSEKEQQLLSTLKCQEEELRKMQEVCEQNQQLLQENSAIKQKLTLLQVASKQKPHLTRNIFQSPDSSFEYIPPKPKPCRIKEKCLEQSFAVEGLQYYSEPSVAEQDNEDSDDHADEEWIPTKLVKVSKKSIQGCSCKGWCGNKQCGCRKQKSDCNVSCSCDPTKCRNRHQNQDNSDAIELNQDSENSFKLEDPTEVTSGLSFFHPICATPSSKILKEMCDADQVQLKQPVFVSSSDHPELKSIASESQENKAIGKKKKRALASNTSFFSGCSPIQEESH

Target ORF information:

RefSeq Version	XM_017318397.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 4 (Kif4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318397.2

ORF Insert Sequence:

ATGGATGAAG CTGCTGCTTG CATGACTGCA ACCATTGATA CTGCAGGGGA AGCAGACACT 
CAAGTGCAAA GCAGCCCAGA TACCAGCAGG TCTTCTGATG TTTTCAGCAC TCAGCATGCC 
CTACGCCAAG CTCAGATGTC CAAGGAGCTG ATTGAGTTGA ATAAAGCACT TGCACTGAAG 
GAAGCCCTAG CTAAGAAGAT GACTCAGAAT GACAACCAAC TCCAGCCCAT TCAGTTCCAG 
TACCAGGATA ATATTAAAAA TCTAGAATCA GAAGTCCTCA GTCTACAGAG GGAAAAAGAA 
GAACTGGTTC TTGAACTTCA AACAGCAAAG AAGGATGCCA ACCAAGCCAA GTTGAGTGAG 
CGCCGTCGTA AACGTCTTCA GGAACTGGAG GGTCAAATAG CTGATTTGAA AAAGAAACTG 
CAAGAACAGT CCAAACTTCT GAAGCTGAAG GAATCCACAG AGCATACTGT CTCCAAGCTG 
AACCAGGAGA TTCGGATGAT GAAAAACCAG CGAGTCCAGT TAATGCGTCA AATGAAAGAG 
GATGCTGAGA AGTTTAGGCA ATGGAAGCAG CAAAAAGACA AAGAAGTAAT CCAGCTAAAA 
GAACGGGATC GTAAAAGGCA ATATGAGCTG CTCAAACTTG AAAGAAACTT TCAGAAGCAG 
TCTAATGTGC TCAGACGTAA AACTGAGGAG GCAGCAGCTG CCAACAAACG TCTTAAGGAT 
GCTCTGCAAA AGCAAAAGGA GGTTGCAGAA AAACGCAAAG AGACTCAGAG CCGTGGGATG 
GAAAGCACTG CAGCTAGAAT GAAGAATTGG CTTGGAAATG AAATTGAGGT TATGGTCAGT 
ACTGAGGAAG CCAAGCGACA TCTGAATGGC CTTCTTGAAG AGAGAAAGAT CCTGGCTCAG 
GATGTGGCAC AACTCAAAGA GAAAAGAGAA TCTGGAGAGA ACCCACCTCT TAAACTCCGA 
AGGCGCACAT TTTCCTACGA TGAAATACAT GGTCAAGATT CAGGAGCAGA GGATTCTATT 
GCCAAGCAGA TTGAAAGCCT AGAGACCGAA TTGGAGCTCA GGAGTGCGCA GATTGCGGAC 
CTACAGCAAA AGCTGCTGGA TGCTGAAAGT GAAGACCGGC CGAAACAGCG CTGGGAGAGT 
ATCGCCACAA TTCTCGAAGC CAAATGTGCC ATAAAGTATT TAGTGGGAGA GCTCGTCTCC 
TCCAAAATAC TGGTGAGCAA ACTAGAAAGC AGCCTGAATC AGAGCAAGGC CAGCTGTATT 
GACGTGCAGA AGATGCTGTT TGAGGAGCAA AATCATTTTG CTAAGATAGA GACAGAGTTA 
AAAGAAGAGC TGGTCAAAGT GGAGCAACAG CACCAAGAAA AGGTGTTGTA CCTTCTCAGC 
CAGCTGCAGC AATCCCAGAT GACAGAGAAG CAGCTGGAGG AGTCAGTTAG TGAAAAGGAA 
CAGCAGCTGC TGAGCACACT AAAATGTCAG GAGGAAGAGC TTAGGAAGAT GCAAGAAGTG 
TGTGAGCAAA ATCAGCAGCT TCTCCAAGAA AACAGTGCCA TCAAGCAGAA ACTGACCCTA 
CTCCAAGTAG CCAGCAAACA GAAACCCCAT CTCACAAGAA ATATCTTCCA GTCTCCAGAC 
TCTTCCTTTG AATATATTCC ACCTAAGCCC AAACCTTGCC GCATTAAGGA AAAATGCCTA 
GAGCAAAGCT TCGCCGTCGA GGGGCTGCAG TATTATTCAG AGCCTTCTGT GGCCGAGCAG 
GACAATGAGG ACAGTGACGA CCATGCTGAT GAGGAGTGGA TCCCAACAAA ACTAGTCAAG 
GTGTCCAAGA AGAGCATCCA AGGGTGTTCC TGCAAAGGTT GGTGCGGGAA CAAGCAATGT 
GGATGCAGAA AGCAAAAGTC AGACTGTAAT GTGTCCTGCA GCTGTGACCC CACAAAGTGT 
CGCAACCGCC ATCAGAACCA GGACAATTCA GATGCTATTG AGCTAAACCA AGATTCTGAA 
AACTCTTTCA AACTGGAAGA TCCCACAGAG GTGACCTCAG GCTTAAGCTT CTTCCACCCT 
ATCTGTGCAA CTCCCAGTAG CAAGATCCTA AAAGAAATGT GTGATGCAGA TCAGGTGCAG 
CTGAAGCAGC CCGTGTTTGT CTCTTCTTCT GACCACCCAG AACTGAAGTC TATAGCGTCA 
GAATCACAAG AAAATAAGGC CATAGGGAAG AAGAAGAAGC GAGCTCTGGC CAGCAATACC 
AGCTTCTTCT CTGGCTGCTC TCCTATCCAA GAAGAGTCCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif4 ( NM_008446.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032472.2 Mus musculus kinesin family member 4 (Kif4), mRNA.

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CloneID	OMu01939
Clone ID Related Accession (Same CDS sequence)	NM_008446.2
Accession Version	NM_008446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	chromosome-associated kinesin KIF4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF742002.1, BC050946.1 and BB091691.1. On Dec 11, 2007 this sequence version replaced NM_008446.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050946.1, AK171938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGAAG AGGTGAAGGG GATTCCCGTA AGAGTGGCAC TGCGTTGTCG CCCCCTCGTC 
TCGAAAGAGA TTAAGGAGGG CTGCCAGACG TGCCTTTCCT TTGTGCCCGG AGAGCCTCAG 
GTGGTGGTTG GTAATGATAA ATCATTTACC TACGATTTTG TGTTTGACCC CTCTACTGAA 
CAGGAAGAGG TCTTTAATAC AGCAGTAGCT CCACTCATAA AAGGCGTGTT TAAAGGATAC 
AATGCAACTG TCCTGGCCTA TGGGCAGACT GGATCTGGAA AAACGTATTC AATGGGAGGT 
GCATACACTG CAGAACAAGA ACATGACTCA GCCATTGGGG TTATTCCCAG GGTAATACAA 
CTGCTCTTCA AAGAAATTAA TAAAAAGAGT GACTTCGAAT TTACTCTGAA AGTGTCTTAC 
TTGGAGATCT ATAATGAAGA AATTTTGGAC CTTCTATGTT CATCTCGTGA GAAAGCTACT 
CAAATAAATA TCCGGGAGGA CCCTAAGGAA GGCATAAAGA TTGTGGGACT GACAGAGAAG 
ACTGTGCTGG TGGCCTCGGA CACCGTTTCC TGTCTAGAGC AGGGTAACAA CTCCAGGACT 
GTGGCCTCCA CAGCTATGAA CTCCCAGTCA TCTCGATCTC ATGCCATCTT TACAATCTCC 
ATTGAGCAAA GGAAGAAGAA TGACAAAAAT AGCAGCTTTC GTTCCAAGCT GCATCTTGTA 
GACCTCGCTG GATCAGAAAG ACAGAAGAAA ACCAAGGCTG AGGGAGATCG ACTGAGAGAG 
GGTATTAATA TTAATCGAGG CCTTCTGTGC TTGGGGAATG TAATCAGTGC TCTTGGAGAT 
GATAAAAAGG GTAACTTTGT GCCCTACAGA GATTCCAAGC TGACTCGACT GCTTCAAGAT 
TCTCTAGGAG GCAATAGCCA CACTCTTATG ATTGCCTGTG TGAGTCCTGC TGATTCCAAT 
CTAGAAGAAA CATTAAATAC CCTTCGCTAT GCTGACAGAG CAAGAAAAAT CAAGAACAAA 
CCTATTATTA ATATTGATCC CCAGGCAGCT GAACTAAATC ATCTTAAACA ACAGGTCCAA 
CAGCTGCAGA TCTTGCTGCT ACAAGCCCAT GGAGGCACCT TGCCTGGAGA TATAAATGTG 
GAACCATCAG AAAATCTTCA GTCCCTGATG GAGAAGAATC AGTCTCTGGT GGAAGAGAAT 
GAAAAATTAA GTCGTGGTCT GAGTGAGGCA GCTGGTCAAA CAGCCCAGAT GCTGGAGAGG 
ATCATTCTGA CAGAGCAAGC AAATGAGAAA ATGAATGCCA AGCTAGAAGA ACTCAGGCGG 
CATGCGGCCT GTAAAGTGGA TCTTCAAAAG CTCGTGGAGA CATTGGAAGA CCAGGAGTTA 
AAAGAAAACA TAGAGATAAT TTGCAACCTA CAACAAGTGA TTGCCCAGCT ATCTGATGAA 
GCTGCTGCTT GCATGACTGC AACCATTGAT ACTGCAGGGG AAGCAGACAC TCAAGTGCAA 
AGCAGCCCAG ATACCAGCAG GTCTTCTGAT GTTTTCAGCA CTCAGCATGC CCTACGCCAA 
GCTCAGATGT CCAAGGAGCT GATTGAGTTG AATAAAGCAC TTGCACTGAA GGAAGCCCTA 
GCTAAGAAGA TGACTCAGAA TGACAACCAA CTCCAGCCCA TTCAGTTCCA GTACCAGGAT 
AATATTAAAA ATCTAGAATC AGAAGTCCTC AGTCTACAGA GGGAAAAAGA AGAACTGGTT 
CTTGAACTTC AAACAGCAAA GAAGGATGCC AACCAAGCCA AGTTGAGTGA GCGCCGTCGT 
AAACGTCTTC AGGAACTGGA GGGTCAAATA GCTGATTTGA AAAAGAAACT GCAAGAACAG 
TCCAAACTTC TGAAGCTGAA GGAATCCACA GAGCATACTG TCTCCAAGCT GAACCAGGAG 
ATTCGGATGA TGAAAAACCA GCGAGTCCAG TTAATGCGTC AAATGAAAGA GGATGCTGAG 
AAGTTTAGGC AATGGAAGCA GCAAAAAGAC AAAGAAGTAA TCCAGCTAAA AGAACGGGAT 
CGTAAAAGGC AATATGAGCT GCTCAAACTT GAAAGAAACT TTCAGAAGCA GTCTAATGTG 
CTCAGACGTA AAACTGAGGA GGCAGCAGCT GCCAACAAAC GTCTTAAGGA TGCTCTGCAA 
AAGCAAAAGG AGGTTGCAGA AAAACGCAAA GAGACTCAGA GCCGTGGGAT GGAAAGCACT 
GCAGCTAGAA TGAAGAATTG GCTTGGAAAT GAAATTGAGG TTATGGTCAG TACTGAGGAA 
GCCAAGCGAC ATCTGAATGG CCTTCTTGAA GAGAGAAAGA TCCTGGCTCA GGATGTGGCA 
CAACTCAAAG AGAAAAGAGA ATCTGGAGAG AACCCACCTC TTAAACTCCG AAGGCGCACA 
TTTTCCTACG ATGAAATACA TGGTCAAGAT TCAGGAGCAG AGGATTCTAT TGCCAAGCAG 
ATTGAAAGCC TAGAGACCGA ATTGGAGCTC AGGAGTGCGC AGATTGCGGA CCTACAGCAA 
AAGCTGCTGG ATGCTGAAAG TGAAGACCGG CCGAAACAGC GCTGGGAGAG TATCGCCACA 
ATTCTCGAAG CCAAATGTGC CATAAAGTAT TTAGTGGGAG AGCTCGTCTC CTCCAAAATA 
CTGGTGAGCA AACTAGAAAG CAGCCTGAAT CAGAGCAAGG CCAGCTGTAT TGACGTGCAG 
AAGATGCTGT TTGAGGAGCA AAATCATTTT GCTAAGATAG AGACAGAGTT AAAAGAAGAG 
CTGGTCAAAG TGGAGCAACA GCACCAAGAA AAGGTGTTGT ACCTTCTCAG CCAGCTGCAG 
CAATCCCAGA TGACAGAGAA GCAGCTGGAG GAGTCAGTTA GTGAAAAGGA ACAGCAGCTG 
CTGAGCACAC TAAAATGTCA GGAGGAAGAG CTTAGGAAGA TGCAAGAAGT GTGTGAGCAA 
AATCAGCAGC TTCTCCAAGA AAACAGTGCC ATCAAGCAGA AACTGACCCT ACTCCAAGTA 
GCCAGCAAAC AGAAACCCCA TCTCACAAGA AATATCTTCC AGTCTCCAGA CTCTTCCTTT 
GAATATATTC CACCTAAGCC CAAACCTTGC CGCATTAAGG AAAAATGCCT AGAGCAAAGC 
TTCGCCGTCG AGGGGCTGCA GTATTATTCA GAGCCTTCTG TGGCCGAGCA GGACAATGAG 
GACAGTGACG ACCATGCTGA TGAGGAGTGG ATCCCAACAA AACTAGTCAA GGTGTCCAAG 
AAGAGCATCC AAGGGTGTTC CTGCAAAGGT TGGTGCGGGA ACAAGCAATG TGGATGCAGA 
AAGCAAAAGT CAGACTGTAA TGTGTCCTGC AGCTGTGACC CCACAAAGTG TCGCAACCGC 
CATCAGAACC AGGACAATTC AGATGCTATT GAGCTAAACC AAGATTCTGA AAACTCTTTC 
AAACTGGAAG ATCCCACAGA GGTGACCTCA GGCTTAAGCT TCTTCCACCC TATCTGTGCA 
ACTCCCAGTA GCAAGATCCT AAAAGAAATG TGTGATGCAG ATCAGGTGCA GCTGAAGCAG 
CCCGTGTTTG TCTCTTCTTC TGACCACCCA GAACTGAAGT CTATAGCGTC AGAATCACAA 
GAAAATAAGG CCATAGGGAA GAAGAAGAAG CGAGCTCTGG CCAGCAATAC CAGCTTCTTC 
TCTGGCTGCT CTCCTATCCA AGAAGAGTCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032472.2
CDS	405..4100
Translation	MKEEVKGIPVRVALRCRPLVSKEIKEGCQTCLSFVPGEPQVVVGNDKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQEHDSAIGVIPRVIQLLFKEINKKSDFEFTLKVSYLEIYNEEILDLLCSSREKATQINIREDPKEGIKIVGLTEKTVLVASDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISIEQRKKNDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLREGININRGLLCLGNVISALGDDKKGNFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIINIDPQAAELNHLKQQVQQLQILLLQAHGGTLPGDINVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANEKMNAKLEELRRHAACKVDLQKLVETLEDQELKENIEIICNLQQVIAQLSDEAAACMTATIDTAGEADTQVQSSPDTSRSSDVFSTQHALRQAQMSKELIELNKALALKEALAKKMTQNDNQLQPIQFQYQDNIKNLESEVLSLQREKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLQEQSKLLKLKESTEHTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQQKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQKEVAEKRKETQSRGMESTAARMKNWLGNEIEVMVSTEEAKRHLNGLLEERKILAQDVAQLKEKRESGENPPLKLRRRTFSYDEIHGQDSGAEDSIAKQIESLETELELRSAQIADLQQKLLDAESEDRPKQRWESIATILEAKCAIKYLVGELVSSKILVSKLESSLNQSKASCIDVQKMLFEEQNHFAKIETELKEELVKVEQQHQEKVLYLLSQLQQSQMTEKQLEESVSEKEQQLLSTLKCQEEELRKMQEVCEQNQQLLQENSAIKQKLTLLQVASKQKPHLTRNIFQSPDSSFEYIPPKPKPCRIKEKCLEQSFAVEGLQYYSEPSVAEQDNEDSDDHADEEWIPTKLVKVSKKSIQGCSCKGWCGNKQCGCRKQKSDCNVSCSCDPTKCRNRHQNQDNSDAIELNQDSENSFKLEDPTEVTSGLSFFHPICATPSSKILKEMCDADQVQLKQPVFVSSSDHPELKSIASESQENKAIGKKKKRALASNTSFFSGCSPIQEESH

Target ORF information:

RefSeq Version	NM_008446.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 4 (Kif4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008446.2

ORF Insert Sequence:

ATGAAAGAAG AGGTGAAGGG GATTCCCGTA AGAGTGGCAC TGCGTTGTCG CCCCCTCGTC 
TCGAAAGAGA TTAAGGAGGG CTGCCAGACG TGCCTTTCCT TTGTGCCCGG AGAGCCTCAG 
GTGGTGGTTG GTAATGATAA ATCATTTACC TACGATTTTG TGTTTGACCC CTCTACTGAA 
CAGGAAGAGG TCTTTAATAC AGCAGTAGCT CCACTCATAA AAGGCGTGTT TAAAGGATAC 
AATGCAACTG TCCTGGCCTA TGGGCAGACT GGATCTGGAA AAACGTATTC AATGGGAGGT 
GCATACACTG CAGAACAAGA ACATGACTCA GCCATTGGGG TTATTCCCAG GGTAATACAA 
CTGCTCTTCA AAGAAATTAA TAAAAAGAGT GACTTCGAAT TTACTCTGAA AGTGTCTTAC 
TTGGAGATCT ATAATGAAGA AATTTTGGAC CTTCTATGTT CATCTCGTGA GAAAGCTACT 
CAAATAAATA TCCGGGAGGA CCCTAAGGAA GGCATAAAGA TTGTGGGACT GACAGAGAAG 
ACTGTGCTGG TGGCCTCGGA CACCGTTTCC TGTCTAGAGC AGGGTAACAA CTCCAGGACT 
GTGGCCTCCA CAGCTATGAA CTCCCAGTCA TCTCGATCTC ATGCCATCTT TACAATCTCC 
ATTGAGCAAA GGAAGAAGAA TGACAAAAAT AGCAGCTTTC GTTCCAAGCT GCATCTTGTA 
GACCTCGCTG GATCAGAAAG ACAGAAGAAA ACCAAGGCTG AGGGAGATCG ACTGAGAGAG 
GGTATTAATA TTAATCGAGG CCTTCTGTGC TTGGGGAATG TAATCAGTGC TCTTGGAGAT 
GATAAAAAGG GTAACTTTGT GCCCTACAGA GATTCCAAGC TGACTCGACT GCTTCAAGAT 
TCTCTAGGAG GCAATAGCCA CACTCTTATG ATTGCCTGTG TGAGTCCTGC TGATTCCAAT 
CTAGAAGAAA CATTAAATAC CCTTCGCTAT GCTGACAGAG CAAGAAAAAT CAAGAACAAA 
CCTATTATTA ATATTGATCC CCAGGCAGCT GAACTAAATC ATCTTAAACA ACAGGTCCAA 
CAGCTGCAGA TCTTGCTGCT ACAAGCCCAT GGAGGCACCT TGCCTGGAGA TATAAATGTG 
GAACCATCAG AAAATCTTCA GTCCCTGATG GAGAAGAATC AGTCTCTGGT GGAAGAGAAT 
GAAAAATTAA GTCGTGGTCT GAGTGAGGCA GCTGGTCAAA CAGCCCAGAT GCTGGAGAGG 
ATCATTCTGA CAGAGCAAGC AAATGAGAAA ATGAATGCCA AGCTAGAAGA ACTCAGGCGG 
CATGCGGCCT GTAAAGTGGA TCTTCAAAAG CTCGTGGAGA CATTGGAAGA CCAGGAGTTA 
AAAGAAAACA TAGAGATAAT TTGCAACCTA CAACAAGTGA TTGCCCAGCT ATCTGATGAA 
GCTGCTGCTT GCATGACTGC AACCATTGAT ACTGCAGGGG AAGCAGACAC TCAAGTGCAA 
AGCAGCCCAG ATACCAGCAG GTCTTCTGAT GTTTTCAGCA CTCAGCATGC CCTACGCCAA 
GCTCAGATGT CCAAGGAGCT GATTGAGTTG AATAAAGCAC TTGCACTGAA GGAAGCCCTA 
GCTAAGAAGA TGACTCAGAA TGACAACCAA CTCCAGCCCA TTCAGTTCCA GTACCAGGAT 
AATATTAAAA ATCTAGAATC AGAAGTCCTC AGTCTACAGA GGGAAAAAGA AGAACTGGTT 
CTTGAACTTC AAACAGCAAA GAAGGATGCC AACCAAGCCA AGTTGAGTGA GCGCCGTCGT 
AAACGTCTTC AGGAACTGGA GGGTCAAATA GCTGATTTGA AAAAGAAACT GCAAGAACAG 
TCCAAACTTC TGAAGCTGAA GGAATCCACA GAGCATACTG TCTCCAAGCT GAACCAGGAG 
ATTCGGATGA TGAAAAACCA GCGAGTCCAG TTAATGCGTC AAATGAAAGA GGATGCTGAG 
AAGTTTAGGC AATGGAAGCA GCAAAAAGAC AAAGAAGTAA TCCAGCTAAA AGAACGGGAT 
CGTAAAAGGC AATATGAGCT GCTCAAACTT GAAAGAAACT TTCAGAAGCA GTCTAATGTG 
CTCAGACGTA AAACTGAGGA GGCAGCAGCT GCCAACAAAC GTCTTAAGGA TGCTCTGCAA 
AAGCAAAAGG AGGTTGCAGA AAAACGCAAA GAGACTCAGA GCCGTGGGAT GGAAAGCACT 
GCAGCTAGAA TGAAGAATTG GCTTGGAAAT GAAATTGAGG TTATGGTCAG TACTGAGGAA 
GCCAAGCGAC ATCTGAATGG CCTTCTTGAA GAGAGAAAGA TCCTGGCTCA GGATGTGGCA 
CAACTCAAAG AGAAAAGAGA ATCTGGAGAG AACCCACCTC TTAAACTCCG AAGGCGCACA 
TTTTCCTACG ATGAAATACA TGGTCAAGAT TCAGGAGCAG AGGATTCTAT TGCCAAGCAG 
ATTGAAAGCC TAGAGACCGA ATTGGAGCTC AGGAGTGCGC AGATTGCGGA CCTACAGCAA 
AAGCTGCTGG ATGCTGAAAG TGAAGACCGG CCGAAACAGC GCTGGGAGAG TATCGCCACA 
ATTCTCGAAG CCAAATGTGC CATAAAGTAT TTAGTGGGAG AGCTCGTCTC CTCCAAAATA 
CTGGTGAGCA AACTAGAAAG CAGCCTGAAT CAGAGCAAGG CCAGCTGTAT TGACGTGCAG 
AAGATGCTGT TTGAGGAGCA AAATCATTTT GCTAAGATAG AGACAGAGTT AAAAGAAGAG 
CTGGTCAAAG TGGAGCAACA GCACCAAGAA AAGGTGTTGT ACCTTCTCAG CCAGCTGCAG 
CAATCCCAGA TGACAGAGAA GCAGCTGGAG GAGTCAGTTA GTGAAAAGGA ACAGCAGCTG 
CTGAGCACAC TAAAATGTCA GGAGGAAGAG CTTAGGAAGA TGCAAGAAGT GTGTGAGCAA 
AATCAGCAGC TTCTCCAAGA AAACAGTGCC ATCAAGCAGA AACTGACCCT ACTCCAAGTA 
GCCAGCAAAC AGAAACCCCA TCTCACAAGA AATATCTTCC AGTCTCCAGA CTCTTCCTTT 
GAATATATTC CACCTAAGCC CAAACCTTGC CGCATTAAGG AAAAATGCCT AGAGCAAAGC 
TTCGCCGTCG AGGGGCTGCA GTATTATTCA GAGCCTTCTG TGGCCGAGCA GGACAATGAG 
GACAGTGACG ACCATGCTGA TGAGGAGTGG ATCCCAACAA AACTAGTCAA GGTGTCCAAG 
AAGAGCATCC AAGGGTGTTC CTGCAAAGGT TGGTGCGGGA ACAAGCAATG TGGATGCAGA 
AAGCAAAAGT CAGACTGTAA TGTGTCCTGC AGCTGTGACC CCACAAAGTG TCGCAACCGC 
CATCAGAACC AGGACAATTC AGATGCTATT GAGCTAAACC AAGATTCTGA AAACTCTTTC 
AAACTGGAAG ATCCCACAGA GGTGACCTCA GGCTTAAGCT TCTTCCACCC TATCTGTGCA 
ACTCCCAGTA GCAAGATCCT AAAAGAAATG TGTGATGCAG ATCAGGTGCA GCTGAAGCAG 
CCCGTGTTTG TCTCTTCTTC TGACCACCCA GAACTGAAGT CTATAGCGTC AGAATCACAA 
GAAAATAAGG CCATAGGGAA GAAGAAGAAG CGAGCTCTGG CCAGCAATAC CAGCTTCTTC 
TCTGGCTGCT CTCCTATCCA AGAAGAGTCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif4 ( XM_006527855.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527918.1 Mus musculus kinesin family member 4 (Kif4), transcript variant X1, mRNA.

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CloneID	OMu56167
Clone ID Related Accession (Same CDS sequence)	XM_006527855.3 , XM_006527855.4
Accession Version	XM_006527855.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromosome-associated kinesin KIF4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527855.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAG AGGTGAAGGG GATTCCCGTA AGAGTGGCAC TGCGTTGTCG CCCCCTCGTC 
TCGAAAGAGA TTAAGGAGGG CTGCCAGACG TGCCTTTCCT TTGTGCCCGG AGAGCCTCAG 
GTGGTGGTTG GTAATGATAA ATCATTTACC TACGATTTTG TGTTTGACCC CTCTACTGAA 
CAGGAAGAGG TCTTTAATAC AGCAGTAGCT CCACTCATAA AAGGCGTGTT TAAAGGATAC 
AATGCAACTG TCCTGGCCTA TGGGCAGACT GGATCTGGAA AAACGTATTC AATGGGAGGT 
GCATACACTG CAGAACAAGA ACATGACTCA GCCATTGGGG TTATTCCCAG GGTAATACAA 
CTGCTCTTCA AAGAAATTAA TAAAAAGAGT GACTTCGAAT TTACTCTGAA AGTGTCTTAC 
TTGGAGATCT ATAATGAAGA AATTTTGGAC CTTCTATGTT CATCTCGTGA GAAAGCTACT 
CAAATAAATA TCCGGGAGGA CCCTAAGGAA GGCATAAAGA TTGTGGGACT GACAGAGAAG 
ACTGTGCTGG TGGCCTCGGA CACCGTTTCC TGTCTAGAGC AGGGTAACAA CTCCAGGACT 
GTGGCCTCCA CAGCTATGAA CTCCCAGTCA TCTCGATCTC ATGCCATCTT TACAATCTCC 
ATTGAGCAAA GGAAGAAGAA TGACAAAAAT AGCAGCTTTC GTTCCAAGCT GCATCTTGTA 
GACCTCGCTG GATCAGAAAG ACAGAAGAAA ACCAAGGCTG AGGGAGATCG ACTGAGAGAG 
GGTATTAATA TTAATCGAGG CCTTCTGTGC TTGGGGAATG TAATCAGTGC TCTTGGAGAT 
GATAAAAAGG GTAACTTTGT GCCCTACAGA GATTCCAAGC TGACTCGACT GCTTCAAGAT 
TCTCTAGGAG GCAATAGCCA CACTCTTATG ATTGCCTGTG TGAGTCCTGC TGATTCCAAT 
CTAGAAGAAA CATTAAATAC CCTTCGCTAT GCTGACAGAG CAAGAAAAAT CAAGAACAAA 
CCTATTATTA ATATTGATCC CCAGGCAGCT GAACTAAATC ATCTTAAACA ACAGGTCCAA 
CAGCTGCAGA TCTTGCTGCT ACAAGCCCAT GGAGGCACCT TGCCTGGAGA TATAAATGTG 
GAACCATCAG AAAATCTTCA GTCCCTGATG GAGAAGAATC AGTCTCTGGT GGAAGAGAAT 
GAAAAATTAA GTCGTGGTCT GAGTGAGGCA GCTGGTCAAA CAGCCCAGAT GCTGGAGAGG 
ATCATTCTGA CAGAGCAAGC AAATGAGAAA ATGAATGCCA AGCTAGAAGA ACTCAGGCGG 
CATGCGGCCT GTAAAGTGGA TCTTCAAAAG CTCGTGGAGA CATTGGAAGA CCAGGAGTTA 
AAAGAAAACA TAGAGATAAT TTGCAACCTA CAACAAGTGA TTGCCCAGCT ATCTGATGAA 
GCTGCTGCTT GCATGACTGC AACCATTGAT ACTGCAGGGG AAGCAGACAC TCAAGTGCAA 
AGCAGCCCAG ATACCAGCAG GTCTTCTGAT GTTTTCAGCA CTCAGCATGC CCTACGCCAA 
GCTCAGATGT CCAAGGAGCT GATTGAGTTG AATAAAGCAC TTGCACTGAA GGAAGCCCTA 
GCTAAGAAGA TGACTCAGAA TGACAACCAA CTCCAGCCCA TTCAGTTCCA GTACCAGGAT 
AATATTAAAA ATCTAGAATC AGAAGTCCTC AGTCTACAGA GGGAAAAAGA AGAACTGGTT 
CTTGAACTTC AAACAGCAAA GAAGGATGCC AACCAAGCCA AGTTGAGTGA GCGCCGTCGT 
AAACGTCTTC AGGAACTGGA GGGTCAAATA GCTGATTTGA AAAAGAAACT GCAAGAACAG 
TCCAAACTTC TGAAGCTGAA GGAATCCACA GAGCATACTG TCTCCAAGCT GAACCAGGAG 
ATTCGGATGA TGAAAAACCA GCGAGTCCAG TTAATGCGTC AAATGAAAGA GGATGCTGAG 
AAGTTTAGGC AATGGAAGCA GCAAAAAGAC AAAGAAGTAA TCCAGCTAAA AGAACGGGAT 
CGTAAAAGGC AATATGAGCT GCTCAAACTT GAAAGAAACT TTCAGAAGCA GTCTAATGTG 
CTCAGACGTA AAACTGAGGA GGCAGCAGCT GCCAACAAAC GTCTTAAGGA TGCTCTGCAA 
AAGCAAAAGG AGGTTGCAGA AAAACGCAAA GAGACTCAGA GCCGTGGGAT GGAAAGCACT 
GCAGCTAGAA TGAAGAATTG GCTTGGAAAT GAAATTGAGG TTATGGTCAG TACTGAGGAA 
GCCAAGCGAC ATCTGAATGG CCTTCTTGAA GAGAGAAAGA TCCTGGCTCA GGATGTGGCA 
CAACTCAAAG AGAAAAGAGA ATCTGGAGAG AACCCACCTC TTAAACTCCG AAGGCGCACA 
TTTTCCTACG ATGAAATACA TGGTCAAGAT TCAGGAGCAG AGGATTCTAT TGCCAAGCAG 
ATTGAAAGCC TAGAGACCGA ATTGGAGCTC AGGAGTGCGC AGATTGCGGA CCTACAGCAA 
AAGCTGCTGG ATGCTGAAAG TGAAGACCGG CCGAAACAGC GCTGGGAGAG TATCGCCACA 
ATTCTCGAAG CCAAATGTGC CATAAAGTAT TTAGTGGGAG AGCTCGTCTC CTCCAAAATA 
CTGGTGAGCA AACTAGAAAG CAGCCTGAAT CAGAGCAAGG CCAGCTGTAT TGACGTGCAG 
AAGATGCTGT TTGAGGAGCA AAATCATTTT GCTAAGATAG AGACAGAGTT AAAAGAAGAG 
CTGGTCAAAG TGGAGCAACA GCACCAAGAA AAGGTGTTGT ACCTTCTCAG CCAGCTGCAG 
CAATCCCAGA TGACAGAGAA GCAGCTGGAG GAGTCAGTTA GTGAAAAGGA ACAGCAGCTG 
CTGAGCACAC TAAAATGTCA GGAGGAAGAG CTTAGGAAGA TGCAAGAAGT GTGTGAGCAA 
AATCAGCAGC TTCTCCAAGA AAACAGTGCC ATCAAGCAGC CCAAACCTTG CCGCATTAAG 
GAAAAATGCC TAGAGCAAAG CTTCGCCGTC GAGGGGCTGC AGTATTATTC AGAGCCTTCT 
GTGGCCGAGC AGGACAATGA GGACAGTGAC GACCATGCTG ATGAGGAGTG GATCCCAACA 
AAACTAGTCA AGGTGTCCAA GAAGAGCATC CAAGGGTGTT CCTGCAAAGG TTGGTGCGGG 
AACAAGCAAT GTGGATGCAG AAAGCAAAAG TCAGACTGTA ATGTGTCCTG CAGCTGTGAC 
CCCACAAAGT GTCGCAACCG CCATCAGAAC CAGGACAATT CAGATGCTAT TGAGCTAAAC 
CAAGATTCTG AAAACTCTTT CAAACTGGAA GATCCCACAG AGGTGACCTC AGGCTTAAGC 
TTCTTCCACC CTATCTGTGC AACTCCCAGT AGCAAGATCC TAAAAGAAAT GTGTGATGCA 
GATCAGGTGC AGCTGAAGCA GCCCGTGTTT GTCTCTTCTT CTGACCACCC AGAACTGAAG 
TCTATAGCGT CAGAATCACA AGAAAATAAG GCCATAGGGA AGAAGAAGAA GCGAGCTCTG 
GCCAGCAATA CCAGCTTCTT CTCTGGCTGC TCTCCTATCC AAGAAGAGTC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527918.1
CDS	361..3957
Translation	MKEEVKGIPVRVALRCRPLVSKEIKEGCQTCLSFVPGEPQVVVGNDKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQEHDSAIGVIPRVIQLLFKEINKKSDFEFTLKVSYLEIYNEEILDLLCSSREKATQINIREDPKEGIKIVGLTEKTVLVASDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISIEQRKKNDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLREGININRGLLCLGNVISALGDDKKGNFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIINIDPQAAELNHLKQQVQQLQILLLQAHGGTLPGDINVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANEKMNAKLEELRRHAACKVDLQKLVETLEDQELKENIEIICNLQQVIAQLSDEAAACMTATIDTAGEADTQVQSSPDTSRSSDVFSTQHALRQAQMSKELIELNKALALKEALAKKMTQNDNQLQPIQFQYQDNIKNLESEVLSLQREKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLQEQSKLLKLKESTEHTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQQKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQKEVAEKRKETQSRGMESTAARMKNWLGNEIEVMVSTEEAKRHLNGLLEERKILAQDVAQLKEKRESGENPPLKLRRRTFSYDEIHGQDSGAEDSIAKQIESLETELELRSAQIADLQQKLLDAESEDRPKQRWESIATILEAKCAIKYLVGELVSSKILVSKLESSLNQSKASCIDVQKMLFEEQNHFAKIETELKEELVKVEQQHQEKVLYLLSQLQQSQMTEKQLEESVSEKEQQLLSTLKCQEEELRKMQEVCEQNQQLLQENSAIKQPKPCRIKEKCLEQSFAVEGLQYYSEPSVAEQDNEDSDDHADEEWIPTKLVKVSKKSIQGCSCKGWCGNKQCGCRKQKSDCNVSCSCDPTKCRNRHQNQDNSDAIELNQDSENSFKLEDPTEVTSGLSFFHPICATPSSKILKEMCDADQVQLKQPVFVSSSDHPELKSIASESQENKAIGKKKKRALASNTSFFSGCSPIQEESH

Target ORF information:

RefSeq Version	XM_006527855.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 4 (Kif4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527855.4

ORF Insert Sequence:

ATGAAAGAAG AGGTGAAGGG GATTCCCGTA AGAGTGGCAC TGCGTTGTCG CCCCCTCGTC 
TCGAAAGAGA TTAAGGAGGG CTGCCAGACG TGCCTTTCCT TTGTGCCCGG AGAGCCTCAG 
GTGGTGGTTG GTAATGATAA ATCATTTACC TACGATTTTG TGTTTGACCC CTCTACTGAA 
CAGGAAGAGG TCTTTAATAC AGCAGTAGCT CCACTCATAA AAGGCGTGTT TAAAGGATAC 
AATGCAACTG TCCTGGCCTA TGGGCAGACT GGATCTGGAA AAACGTATTC AATGGGAGGT 
GCATACACTG CAGAACAAGA ACATGACTCA GCCATTGGGG TTATTCCCAG GGTAATACAA 
CTGCTCTTCA AAGAAATTAA TAAAAAGAGT GACTTCGAAT TTACTCTGAA AGTGTCTTAC 
TTGGAGATCT ATAATGAAGA AATTTTGGAC CTTCTATGTT CATCTCGTGA GAAAGCTACT 
CAAATAAATA TCCGGGAGGA CCCTAAGGAA GGCATAAAGA TTGTGGGACT GACAGAGAAG 
ACTGTGCTGG TGGCCTCGGA CACCGTTTCC TGTCTAGAGC AGGGTAACAA CTCCAGGACT 
GTGGCCTCCA CAGCTATGAA CTCCCAGTCA TCTCGATCTC ATGCCATCTT TACAATCTCC 
ATTGAGCAAA GGAAGAAGAA TGACAAAAAT AGCAGCTTTC GTTCCAAGCT GCATCTTGTA 
GACCTCGCTG GATCAGAAAG ACAGAAGAAA ACCAAGGCTG AGGGAGATCG ACTGAGAGAG 
GGTATTAATA TTAATCGAGG CCTTCTGTGC TTGGGGAATG TAATCAGTGC TCTTGGAGAT 
GATAAAAAGG GTAACTTTGT GCCCTACAGA GATTCCAAGC TGACTCGACT GCTTCAAGAT 
TCTCTAGGAG GCAATAGCCA CACTCTTATG ATTGCCTGTG TGAGTCCTGC TGATTCCAAT 
CTAGAAGAAA CATTAAATAC CCTTCGCTAT GCTGACAGAG CAAGAAAAAT CAAGAACAAA 
CCTATTATTA ATATTGATCC CCAGGCAGCT GAACTAAATC ATCTTAAACA ACAGGTCCAA 
CAGCTGCAGA TCTTGCTGCT ACAAGCCCAT GGAGGCACCT TGCCTGGAGA TATAAATGTG 
GAACCATCAG AAAATCTTCA GTCCCTGATG GAGAAGAATC AGTCTCTGGT GGAAGAGAAT 
GAAAAATTAA GTCGTGGTCT GAGTGAGGCA GCTGGTCAAA CAGCCCAGAT GCTGGAGAGG 
ATCATTCTGA CAGAGCAAGC AAATGAGAAA ATGAATGCCA AGCTAGAAGA ACTCAGGCGG 
CATGCGGCCT GTAAAGTGGA TCTTCAAAAG CTCGTGGAGA CATTGGAAGA CCAGGAGTTA 
AAAGAAAACA TAGAGATAAT TTGCAACCTA CAACAAGTGA TTGCCCAGCT ATCTGATGAA 
GCTGCTGCTT GCATGACTGC AACCATTGAT ACTGCAGGGG AAGCAGACAC TCAAGTGCAA 
AGCAGCCCAG ATACCAGCAG GTCTTCTGAT GTTTTCAGCA CTCAGCATGC CCTACGCCAA 
GCTCAGATGT CCAAGGAGCT GATTGAGTTG AATAAAGCAC TTGCACTGAA GGAAGCCCTA 
GCTAAGAAGA TGACTCAGAA TGACAACCAA CTCCAGCCCA TTCAGTTCCA GTACCAGGAT 
AATATTAAAA ATCTAGAATC AGAAGTCCTC AGTCTACAGA GGGAAAAAGA AGAACTGGTT 
CTTGAACTTC AAACAGCAAA GAAGGATGCC AACCAAGCCA AGTTGAGTGA GCGCCGTCGT 
AAACGTCTTC AGGAACTGGA GGGTCAAATA GCTGATTTGA AAAAGAAACT GCAAGAACAG 
TCCAAACTTC TGAAGCTGAA GGAATCCACA GAGCATACTG TCTCCAAGCT GAACCAGGAG 
ATTCGGATGA TGAAAAACCA GCGAGTCCAG TTAATGCGTC AAATGAAAGA GGATGCTGAG 
AAGTTTAGGC AATGGAAGCA GCAAAAAGAC AAAGAAGTAA TCCAGCTAAA AGAACGGGAT 
CGTAAAAGGC AATATGAGCT GCTCAAACTT GAAAGAAACT TTCAGAAGCA GTCTAATGTG 
CTCAGACGTA AAACTGAGGA GGCAGCAGCT GCCAACAAAC GTCTTAAGGA TGCTCTGCAA 
AAGCAAAAGG AGGTTGCAGA AAAACGCAAA GAGACTCAGA GCCGTGGGAT GGAAAGCACT 
GCAGCTAGAA TGAAGAATTG GCTTGGAAAT GAAATTGAGG TTATGGTCAG TACTGAGGAA 
GCCAAGCGAC ATCTGAATGG CCTTCTTGAA GAGAGAAAGA TCCTGGCTCA GGATGTGGCA 
CAACTCAAAG AGAAAAGAGA ATCTGGAGAG AACCCACCTC TTAAACTCCG AAGGCGCACA 
TTTTCCTACG ATGAAATACA TGGTCAAGAT TCAGGAGCAG AGGATTCTAT TGCCAAGCAG 
ATTGAAAGCC TAGAGACCGA ATTGGAGCTC AGGAGTGCGC AGATTGCGGA CCTACAGCAA 
AAGCTGCTGG ATGCTGAAAG TGAAGACCGG CCGAAACAGC GCTGGGAGAG TATCGCCACA 
ATTCTCGAAG CCAAATGTGC CATAAAGTAT TTAGTGGGAG AGCTCGTCTC CTCCAAAATA 
CTGGTGAGCA AACTAGAAAG CAGCCTGAAT CAGAGCAAGG CCAGCTGTAT TGACGTGCAG 
AAGATGCTGT TTGAGGAGCA AAATCATTTT GCTAAGATAG AGACAGAGTT AAAAGAAGAG 
CTGGTCAAAG TGGAGCAACA GCACCAAGAA AAGGTGTTGT ACCTTCTCAG CCAGCTGCAG 
CAATCCCAGA TGACAGAGAA GCAGCTGGAG GAGTCAGTTA GTGAAAAGGA ACAGCAGCTG 
CTGAGCACAC TAAAATGTCA GGAGGAAGAG CTTAGGAAGA TGCAAGAAGT GTGTGAGCAA 
AATCAGCAGC TTCTCCAAGA AAACAGTGCC ATCAAGCAGC CCAAACCTTG CCGCATTAAG 
GAAAAATGCC TAGAGCAAAG CTTCGCCGTC GAGGGGCTGC AGTATTATTC AGAGCCTTCT 
GTGGCCGAGC AGGACAATGA GGACAGTGAC GACCATGCTG ATGAGGAGTG GATCCCAACA 
AAACTAGTCA AGGTGTCCAA GAAGAGCATC CAAGGGTGTT CCTGCAAAGG TTGGTGCGGG 
AACAAGCAAT GTGGATGCAG AAAGCAAAAG TCAGACTGTA ATGTGTCCTG CAGCTGTGAC 
CCCACAAAGT GTCGCAACCG CCATCAGAAC CAGGACAATT CAGATGCTAT TGAGCTAAAC 
CAAGATTCTG AAAACTCTTT CAAACTGGAA GATCCCACAG AGGTGACCTC AGGCTTAAGC 
TTCTTCCACC CTATCTGTGC AACTCCCAGT AGCAAGATCC TAAAAGAAAT GTGTGATGCA 
GATCAGGTGC AGCTGAAGCA GCCCGTGTTT GTCTCTTCTT CTGACCACCC AGAACTGAAG 
TCTATAGCGT CAGAATCACA AGAAAATAAG GCCATAGGGA AGAAGAAGAA GCGAGCTCTG 
GCCAGCAATA CCAGCTTCTT CTCTGGCTGC TCTCCTATCC AAGAAGAGTC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif4 ( XM_006527855.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527918.1 Mus musculus kinesin family member 4 (Kif4), transcript variant X1, mRNA.

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CloneID	OMu56167
Clone ID Related Accession (Same CDS sequence)	XM_006527855.3 , XM_006527855.4
Accession Version	XM_006527855.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromosome-associated kinesin KIF4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527855.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAG AGGTGAAGGG GATTCCCGTA AGAGTGGCAC TGCGTTGTCG CCCCCTCGTC 
TCGAAAGAGA TTAAGGAGGG CTGCCAGACG TGCCTTTCCT TTGTGCCCGG AGAGCCTCAG 
GTGGTGGTTG GTAATGATAA ATCATTTACC TACGATTTTG TGTTTGACCC CTCTACTGAA 
CAGGAAGAGG TCTTTAATAC AGCAGTAGCT CCACTCATAA AAGGCGTGTT TAAAGGATAC 
AATGCAACTG TCCTGGCCTA TGGGCAGACT GGATCTGGAA AAACGTATTC AATGGGAGGT 
GCATACACTG CAGAACAAGA ACATGACTCA GCCATTGGGG TTATTCCCAG GGTAATACAA 
CTGCTCTTCA AAGAAATTAA TAAAAAGAGT GACTTCGAAT TTACTCTGAA AGTGTCTTAC 
TTGGAGATCT ATAATGAAGA AATTTTGGAC CTTCTATGTT CATCTCGTGA GAAAGCTACT 
CAAATAAATA TCCGGGAGGA CCCTAAGGAA GGCATAAAGA TTGTGGGACT GACAGAGAAG 
ACTGTGCTGG TGGCCTCGGA CACCGTTTCC TGTCTAGAGC AGGGTAACAA CTCCAGGACT 
GTGGCCTCCA CAGCTATGAA CTCCCAGTCA TCTCGATCTC ATGCCATCTT TACAATCTCC 
ATTGAGCAAA GGAAGAAGAA TGACAAAAAT AGCAGCTTTC GTTCCAAGCT GCATCTTGTA 
GACCTCGCTG GATCAGAAAG ACAGAAGAAA ACCAAGGCTG AGGGAGATCG ACTGAGAGAG 
GGTATTAATA TTAATCGAGG CCTTCTGTGC TTGGGGAATG TAATCAGTGC TCTTGGAGAT 
GATAAAAAGG GTAACTTTGT GCCCTACAGA GATTCCAAGC TGACTCGACT GCTTCAAGAT 
TCTCTAGGAG GCAATAGCCA CACTCTTATG ATTGCCTGTG TGAGTCCTGC TGATTCCAAT 
CTAGAAGAAA CATTAAATAC CCTTCGCTAT GCTGACAGAG CAAGAAAAAT CAAGAACAAA 
CCTATTATTA ATATTGATCC CCAGGCAGCT GAACTAAATC ATCTTAAACA ACAGGTCCAA 
CAGCTGCAGA TCTTGCTGCT ACAAGCCCAT GGAGGCACCT TGCCTGGAGA TATAAATGTG 
GAACCATCAG AAAATCTTCA GTCCCTGATG GAGAAGAATC AGTCTCTGGT GGAAGAGAAT 
GAAAAATTAA GTCGTGGTCT GAGTGAGGCA GCTGGTCAAA CAGCCCAGAT GCTGGAGAGG 
ATCATTCTGA CAGAGCAAGC AAATGAGAAA ATGAATGCCA AGCTAGAAGA ACTCAGGCGG 
CATGCGGCCT GTAAAGTGGA TCTTCAAAAG CTCGTGGAGA CATTGGAAGA CCAGGAGTTA 
AAAGAAAACA TAGAGATAAT TTGCAACCTA CAACAAGTGA TTGCCCAGCT ATCTGATGAA 
GCTGCTGCTT GCATGACTGC AACCATTGAT ACTGCAGGGG AAGCAGACAC TCAAGTGCAA 
AGCAGCCCAG ATACCAGCAG GTCTTCTGAT GTTTTCAGCA CTCAGCATGC CCTACGCCAA 
GCTCAGATGT CCAAGGAGCT GATTGAGTTG AATAAAGCAC TTGCACTGAA GGAAGCCCTA 
GCTAAGAAGA TGACTCAGAA TGACAACCAA CTCCAGCCCA TTCAGTTCCA GTACCAGGAT 
AATATTAAAA ATCTAGAATC AGAAGTCCTC AGTCTACAGA GGGAAAAAGA AGAACTGGTT 
CTTGAACTTC AAACAGCAAA GAAGGATGCC AACCAAGCCA AGTTGAGTGA GCGCCGTCGT 
AAACGTCTTC AGGAACTGGA GGGTCAAATA GCTGATTTGA AAAAGAAACT GCAAGAACAG 
TCCAAACTTC TGAAGCTGAA GGAATCCACA GAGCATACTG TCTCCAAGCT GAACCAGGAG 
ATTCGGATGA TGAAAAACCA GCGAGTCCAG TTAATGCGTC AAATGAAAGA GGATGCTGAG 
AAGTTTAGGC AATGGAAGCA GCAAAAAGAC AAAGAAGTAA TCCAGCTAAA AGAACGGGAT 
CGTAAAAGGC AATATGAGCT GCTCAAACTT GAAAGAAACT TTCAGAAGCA GTCTAATGTG 
CTCAGACGTA AAACTGAGGA GGCAGCAGCT GCCAACAAAC GTCTTAAGGA TGCTCTGCAA 
AAGCAAAAGG AGGTTGCAGA AAAACGCAAA GAGACTCAGA GCCGTGGGAT GGAAAGCACT 
GCAGCTAGAA TGAAGAATTG GCTTGGAAAT GAAATTGAGG TTATGGTCAG TACTGAGGAA 
GCCAAGCGAC ATCTGAATGG CCTTCTTGAA GAGAGAAAGA TCCTGGCTCA GGATGTGGCA 
CAACTCAAAG AGAAAAGAGA ATCTGGAGAG AACCCACCTC TTAAACTCCG AAGGCGCACA 
TTTTCCTACG ATGAAATACA TGGTCAAGAT TCAGGAGCAG AGGATTCTAT TGCCAAGCAG 
ATTGAAAGCC TAGAGACCGA ATTGGAGCTC AGGAGTGCGC AGATTGCGGA CCTACAGCAA 
AAGCTGCTGG ATGCTGAAAG TGAAGACCGG CCGAAACAGC GCTGGGAGAG TATCGCCACA 
ATTCTCGAAG CCAAATGTGC CATAAAGTAT TTAGTGGGAG AGCTCGTCTC CTCCAAAATA 
CTGGTGAGCA AACTAGAAAG CAGCCTGAAT CAGAGCAAGG CCAGCTGTAT TGACGTGCAG 
AAGATGCTGT TTGAGGAGCA AAATCATTTT GCTAAGATAG AGACAGAGTT AAAAGAAGAG 
CTGGTCAAAG TGGAGCAACA GCACCAAGAA AAGGTGTTGT ACCTTCTCAG CCAGCTGCAG 
CAATCCCAGA TGACAGAGAA GCAGCTGGAG GAGTCAGTTA GTGAAAAGGA ACAGCAGCTG 
CTGAGCACAC TAAAATGTCA GGAGGAAGAG CTTAGGAAGA TGCAAGAAGT GTGTGAGCAA 
AATCAGCAGC TTCTCCAAGA AAACAGTGCC ATCAAGCAGC CCAAACCTTG CCGCATTAAG 
GAAAAATGCC TAGAGCAAAG CTTCGCCGTC GAGGGGCTGC AGTATTATTC AGAGCCTTCT 
GTGGCCGAGC AGGACAATGA GGACAGTGAC GACCATGCTG ATGAGGAGTG GATCCCAACA 
AAACTAGTCA AGGTGTCCAA GAAGAGCATC CAAGGGTGTT CCTGCAAAGG TTGGTGCGGG 
AACAAGCAAT GTGGATGCAG AAAGCAAAAG TCAGACTGTA ATGTGTCCTG CAGCTGTGAC 
CCCACAAAGT GTCGCAACCG CCATCAGAAC CAGGACAATT CAGATGCTAT TGAGCTAAAC 
CAAGATTCTG AAAACTCTTT CAAACTGGAA GATCCCACAG AGGTGACCTC AGGCTTAAGC 
TTCTTCCACC CTATCTGTGC AACTCCCAGT AGCAAGATCC TAAAAGAAAT GTGTGATGCA 
GATCAGGTGC AGCTGAAGCA GCCCGTGTTT GTCTCTTCTT CTGACCACCC AGAACTGAAG 
TCTATAGCGT CAGAATCACA AGAAAATAAG GCCATAGGGA AGAAGAAGAA GCGAGCTCTG 
GCCAGCAATA CCAGCTTCTT CTCTGGCTGC TCTCCTATCC AAGAAGAGTC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527918.1
CDS	352..3948
Translation	MKEEVKGIPVRVALRCRPLVSKEIKEGCQTCLSFVPGEPQVVVGNDKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQEHDSAIGVIPRVIQLLFKEINKKSDFEFTLKVSYLEIYNEEILDLLCSSREKATQINIREDPKEGIKIVGLTEKTVLVASDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISIEQRKKNDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLREGININRGLLCLGNVISALGDDKKGNFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIINIDPQAAELNHLKQQVQQLQILLLQAHGGTLPGDINVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANEKMNAKLEELRRHAACKVDLQKLVETLEDQELKENIEIICNLQQVIAQLSDEAAACMTATIDTAGEADTQVQSSPDTSRSSDVFSTQHALRQAQMSKELIELNKALALKEALAKKMTQNDNQLQPIQFQYQDNIKNLESEVLSLQREKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLQEQSKLLKLKESTEHTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQQKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQKEVAEKRKETQSRGMESTAARMKNWLGNEIEVMVSTEEAKRHLNGLLEERKILAQDVAQLKEKRESGENPPLKLRRRTFSYDEIHGQDSGAEDSIAKQIESLETELELRSAQIADLQQKLLDAESEDRPKQRWESIATILEAKCAIKYLVGELVSSKILVSKLESSLNQSKASCIDVQKMLFEEQNHFAKIETELKEELVKVEQQHQEKVLYLLSQLQQSQMTEKQLEESVSEKEQQLLSTLKCQEEELRKMQEVCEQNQQLLQENSAIKQPKPCRIKEKCLEQSFAVEGLQYYSEPSVAEQDNEDSDDHADEEWIPTKLVKVSKKSIQGCSCKGWCGNKQCGCRKQKSDCNVSCSCDPTKCRNRHQNQDNSDAIELNQDSENSFKLEDPTEVTSGLSFFHPICATPSSKILKEMCDADQVQLKQPVFVSSSDHPELKSIASESQENKAIGKKKKRALASNTSFFSGCSPIQEESH

Target ORF information:

RefSeq Version	XM_006527855.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 4 (Kif4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527855.3

ORF Insert Sequence:

ATGAAAGAAG AGGTGAAGGG GATTCCCGTA AGAGTGGCAC TGCGTTGTCG CCCCCTCGTC 
TCGAAAGAGA TTAAGGAGGG CTGCCAGACG TGCCTTTCCT TTGTGCCCGG AGAGCCTCAG 
GTGGTGGTTG GTAATGATAA ATCATTTACC TACGATTTTG TGTTTGACCC CTCTACTGAA 
CAGGAAGAGG TCTTTAATAC AGCAGTAGCT CCACTCATAA AAGGCGTGTT TAAAGGATAC 
AATGCAACTG TCCTGGCCTA TGGGCAGACT GGATCTGGAA AAACGTATTC AATGGGAGGT 
GCATACACTG CAGAACAAGA ACATGACTCA GCCATTGGGG TTATTCCCAG GGTAATACAA 
CTGCTCTTCA AAGAAATTAA TAAAAAGAGT GACTTCGAAT TTACTCTGAA AGTGTCTTAC 
TTGGAGATCT ATAATGAAGA AATTTTGGAC CTTCTATGTT CATCTCGTGA GAAAGCTACT 
CAAATAAATA TCCGGGAGGA CCCTAAGGAA GGCATAAAGA TTGTGGGACT GACAGAGAAG 
ACTGTGCTGG TGGCCTCGGA CACCGTTTCC TGTCTAGAGC AGGGTAACAA CTCCAGGACT 
GTGGCCTCCA CAGCTATGAA CTCCCAGTCA TCTCGATCTC ATGCCATCTT TACAATCTCC 
ATTGAGCAAA GGAAGAAGAA TGACAAAAAT AGCAGCTTTC GTTCCAAGCT GCATCTTGTA 
GACCTCGCTG GATCAGAAAG ACAGAAGAAA ACCAAGGCTG AGGGAGATCG ACTGAGAGAG 
GGTATTAATA TTAATCGAGG CCTTCTGTGC TTGGGGAATG TAATCAGTGC TCTTGGAGAT 
GATAAAAAGG GTAACTTTGT GCCCTACAGA GATTCCAAGC TGACTCGACT GCTTCAAGAT 
TCTCTAGGAG GCAATAGCCA CACTCTTATG ATTGCCTGTG TGAGTCCTGC TGATTCCAAT 
CTAGAAGAAA CATTAAATAC CCTTCGCTAT GCTGACAGAG CAAGAAAAAT CAAGAACAAA 
CCTATTATTA ATATTGATCC CCAGGCAGCT GAACTAAATC ATCTTAAACA ACAGGTCCAA 
CAGCTGCAGA TCTTGCTGCT ACAAGCCCAT GGAGGCACCT TGCCTGGAGA TATAAATGTG 
GAACCATCAG AAAATCTTCA GTCCCTGATG GAGAAGAATC AGTCTCTGGT GGAAGAGAAT 
GAAAAATTAA GTCGTGGTCT GAGTGAGGCA GCTGGTCAAA CAGCCCAGAT GCTGGAGAGG 
ATCATTCTGA CAGAGCAAGC AAATGAGAAA ATGAATGCCA AGCTAGAAGA ACTCAGGCGG 
CATGCGGCCT GTAAAGTGGA TCTTCAAAAG CTCGTGGAGA CATTGGAAGA CCAGGAGTTA 
AAAGAAAACA TAGAGATAAT TTGCAACCTA CAACAAGTGA TTGCCCAGCT ATCTGATGAA 
GCTGCTGCTT GCATGACTGC AACCATTGAT ACTGCAGGGG AAGCAGACAC TCAAGTGCAA 
AGCAGCCCAG ATACCAGCAG GTCTTCTGAT GTTTTCAGCA CTCAGCATGC CCTACGCCAA 
GCTCAGATGT CCAAGGAGCT GATTGAGTTG AATAAAGCAC TTGCACTGAA GGAAGCCCTA 
GCTAAGAAGA TGACTCAGAA TGACAACCAA CTCCAGCCCA TTCAGTTCCA GTACCAGGAT 
AATATTAAAA ATCTAGAATC AGAAGTCCTC AGTCTACAGA GGGAAAAAGA AGAACTGGTT 
CTTGAACTTC AAACAGCAAA GAAGGATGCC AACCAAGCCA AGTTGAGTGA GCGCCGTCGT 
AAACGTCTTC AGGAACTGGA GGGTCAAATA GCTGATTTGA AAAAGAAACT GCAAGAACAG 
TCCAAACTTC TGAAGCTGAA GGAATCCACA GAGCATACTG TCTCCAAGCT GAACCAGGAG 
ATTCGGATGA TGAAAAACCA GCGAGTCCAG TTAATGCGTC AAATGAAAGA GGATGCTGAG 
AAGTTTAGGC AATGGAAGCA GCAAAAAGAC AAAGAAGTAA TCCAGCTAAA AGAACGGGAT 
CGTAAAAGGC AATATGAGCT GCTCAAACTT GAAAGAAACT TTCAGAAGCA GTCTAATGTG 
CTCAGACGTA AAACTGAGGA GGCAGCAGCT GCCAACAAAC GTCTTAAGGA TGCTCTGCAA 
AAGCAAAAGG AGGTTGCAGA AAAACGCAAA GAGACTCAGA GCCGTGGGAT GGAAAGCACT 
GCAGCTAGAA TGAAGAATTG GCTTGGAAAT GAAATTGAGG TTATGGTCAG TACTGAGGAA 
GCCAAGCGAC ATCTGAATGG CCTTCTTGAA GAGAGAAAGA TCCTGGCTCA GGATGTGGCA 
CAACTCAAAG AGAAAAGAGA ATCTGGAGAG AACCCACCTC TTAAACTCCG AAGGCGCACA 
TTTTCCTACG ATGAAATACA TGGTCAAGAT TCAGGAGCAG AGGATTCTAT TGCCAAGCAG 
ATTGAAAGCC TAGAGACCGA ATTGGAGCTC AGGAGTGCGC AGATTGCGGA CCTACAGCAA 
AAGCTGCTGG ATGCTGAAAG TGAAGACCGG CCGAAACAGC GCTGGGAGAG TATCGCCACA 
ATTCTCGAAG CCAAATGTGC CATAAAGTAT TTAGTGGGAG AGCTCGTCTC CTCCAAAATA 
CTGGTGAGCA AACTAGAAAG CAGCCTGAAT CAGAGCAAGG CCAGCTGTAT TGACGTGCAG 
AAGATGCTGT TTGAGGAGCA AAATCATTTT GCTAAGATAG AGACAGAGTT AAAAGAAGAG 
CTGGTCAAAG TGGAGCAACA GCACCAAGAA AAGGTGTTGT ACCTTCTCAG CCAGCTGCAG 
CAATCCCAGA TGACAGAGAA GCAGCTGGAG GAGTCAGTTA GTGAAAAGGA ACAGCAGCTG 
CTGAGCACAC TAAAATGTCA GGAGGAAGAG CTTAGGAAGA TGCAAGAAGT GTGTGAGCAA 
AATCAGCAGC TTCTCCAAGA AAACAGTGCC ATCAAGCAGC CCAAACCTTG CCGCATTAAG 
GAAAAATGCC TAGAGCAAAG CTTCGCCGTC GAGGGGCTGC AGTATTATTC AGAGCCTTCT 
GTGGCCGAGC AGGACAATGA GGACAGTGAC GACCATGCTG ATGAGGAGTG GATCCCAACA 
AAACTAGTCA AGGTGTCCAA GAAGAGCATC CAAGGGTGTT CCTGCAAAGG TTGGTGCGGG 
AACAAGCAAT GTGGATGCAG AAAGCAAAAG TCAGACTGTA ATGTGTCCTG CAGCTGTGAC 
CCCACAAAGT GTCGCAACCG CCATCAGAAC CAGGACAATT CAGATGCTAT TGAGCTAAAC 
CAAGATTCTG AAAACTCTTT CAAACTGGAA GATCCCACAG AGGTGACCTC AGGCTTAAGC 
TTCTTCCACC CTATCTGTGC AACTCCCAGT AGCAAGATCC TAAAAGAAAT GTGTGATGCA 
GATCAGGTGC AGCTGAAGCA GCCCGTGTTT GTCTCTTCTT CTGACCACCC AGAACTGAAG 
TCTATAGCGT CAGAATCACA AGAAAATAAG GCCATAGGGA AGAAGAAGAA GCGAGCTCTG 
GCCAGCAATA CCAGCTTCTT CTCTGGCTGC TCTCCTATCC AAGAAGAGTC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Contribution of epigenetic landscapes and transcription factors to X-chromosome reactivation in the inner cell mass.
Nature communications8(1)1297(2017 Nov)
Borensztein M,Okamoto I,Syx L,Guilbaud G,Picard C,Ancelin K,Galupa R,Diabangouaya P,Servant N,Barillot E,Surani A,Saitou M,Chen CJ,Anastassiadis K,Heard E

Kif4 regulates the expression of VEGFR1 through the PI3K/Akt signaling pathway in RAW264.7 monocytes/macrophages.
International journal of molecular medicine39(5)1285-1290(2017 May)
Xu Y,Luan Y,Liu S,Sun J,Wang K,Cai J,Jiang W,Yang P,Wei F,Qu X

Kif4 Is Essential for Mouse Oocyte Meiosis.
PloS one12(1)e0170650(2017)
Camlin NJ,McLaughlin EA,Holt JE

Kif4 interacts with EB1 and stabilizes microtubules downstream of Rho-mDia in migrating fibroblasts.
PloS one9(3)e91568(2014)
Morris EJ,Nader GP,Ramalingam N,Bartolini F,Gundersen GG

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Kif4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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