Kcnb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnb1
Entrez Gene ID	16500
Full Name	potassium voltage gated channel, Shab-related subfamily, member 1
Synonyms	Kcr1-1,Kv2.1,Shab
General protein information	Preferred Names potassium voltage gated channel, Shab-related subfamily, member 1 Names potassium voltage-gated channel subfamily B member 1 voltage-gated potassium channel subunit Kv2.1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H3\|2 87.22 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017315732.1	XP_017171221.1	potassium voltage-gated channel subfamily B member 1 isoform X1
NM_008420.4	NP_032446.2	potassium voltage-gated channel subfamily B member 1
XM_017315733.2	XP_017171222.1	potassium voltage-gated channel subfamily B member 1 isoform X1
XM_017315733.1	XP_017171222.1	potassium voltage-gated channel subfamily B member 1 isoform X1
XM_017315732.2	XP_017171221.1	potassium voltage-gated channel subfamily B member 1 isoform X1

Pathways from BioSystems

Homologies

	KCNB1	XP_001098678.1
Rattus norvegicus (Norway rat)	Kcnb1	NP_037318.1
Pan troglodytes (chimpanzee)	KCNB1	XP_525349.2
Danio rerio (zebrafish)	kcnb1	XP_682848.4
Bos taurus (cattle)	KCNB1	NP_001179198.1
Gallus gallus (chicken)	KCNB1	XP_425704.2
Canis lupus familiaris (dog)	KCNB1	XP_543042.2
Xenopus tropicalis (tropical clawed frog)	kcnb1	XP_002933211.2
Homo sapiens (human)	KCNB1	NP_004966.1
Mus musculus (house mouse)	Kcnb1	NP_032446.2

Gene Ontology
Bibliography

Related articles in PubMed

Reduced sensory synaptic excitation impairs motor neuron function via Kv2.1 in spinal muscular atrophy.
Fletcher EV, Simon CM, Pagiazitis JG, Chalif JI, Vukojicic A, Drobac E, Wang X, Mentis GZ
Nature neuroscience20(7)905-916(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spinal Musculature Atrophy motor neurons showed a lower surface expression of Kv2.1 potassium channels and reduced spiking ability.
Title: Reduced sensory synaptic excitation impairs motor neuron function via Kv2.1 in spinal muscular atrophy.

KCNB1 oxidation may favor integrin clustering, thereby facilitating the recruitment and activation of FAK and Src/Fyn kinases.
Title: Oxidation of KCNB1 potassium channels triggers apoptotic integrin signaling in the brain.

This study provides the first experimental evidence that oxidation of a K(+) channel constitutes a mechanism of neuronal and cognitive impairment in vertebrates. Specifically, the interaction of KCNB1 channels with reactive oxygen species plays a major role in the etiology of mouse model of traumatic brain injury (TBI), a condition associated with extensive oxidative stress. In addition, a Food and Drug Administration-app
Title: Oxidation of KCNB1 Potassium Channels Causes Neurotoxicity and Cognitive Impairment in a Mouse Model of Traumatic Brain Injury.

The AMIGO1-KCNB1 complex is involved in schizophrenia-related behavioral domains in mice.
Title: AMIGO-Kv2.1 Potassium Channel Complex Is Associated With Schizophrenia-Related Phenotypes.

study supports the concept that transcriptional suppression of KV2.1 by activation of the AKAP150-CaN/NFATc3 signaling axis contributes to enhanced arterial tone during diabetes
Title: Selective down-regulation of KV2.1 function contributes to enhanced arterial tone during diabetes.

The following Kcnb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13702	NM_008420.4 Latest version!	Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$503.30 $719.00
OMu13702	XM_017315733.2 Latest version!	Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu13702	XM_017315732.2 Latest version!	Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu13702	XM_017315733.1	Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$503.30 $719.00
OMu13702	XM_017315732.1	Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$503.30 $719.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnb1 ( NM_008420.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032446.2 Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), mRNA.

Price & Availability↑

CloneID	OMu13702
Clone ID Related Accession (Same CDS sequence)	XM_017315732.1 , NM_008420.4 , XM_017315733.2 , XM_017315733.1 , XM_017315732.2
Accession Version	NM_008420.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	potassium voltage-gated channel subfamily B member 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591854.18, BC061501.1 and AL591711.22. On or before Dec 30, 2011 this sequence version replaced NR_045278.1, NM_008420.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ646377.1, BC061501.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGCGG GCATGACGAA GCATGGCTCG CGCTCCACCA GCTCGCTGCC GCCCGAGCCC 
ATGGAGATCG TGCGCAGCAA GGCGTGCTCG CGCCGGGTGC GCCTCAACGT CGGGGGCCTG 
GCGCACGAGG TGCTGTGGCG CACTCTGGAC CGCCTGCCTC GCACGCGGCT GGGCAAGCTC 
CGGGACTGCA ACACGCACGA CTCTCTGCTC CAGGTGTGCG ACGACTACAG CCTCGAGGAC 
AACGAGTACT TCTTCGACCG CCACCCTGGC GCCTTCACCT CTATTCTCAA TTTCTACCGC 
ACCGGCCGGC TGCACATGAT GGAGGAGATG TGCGCGCTGA GCTTCAGCCA GGAGCTGGAC 
TACTGGGGCA TCGATGAGAT CTACCTGGAG TCCTGCTGCC AGGCCCGCTA CCACCAAAAG 
AAGGAGCAGA TGAACGAGGA GCTGAAGCGG GAGGCTGAGA CGCTGCGGGA GCGGGAGGGC 
GAGGAGTTCG ACAACACGTG CTGTGCTGAG AAGAGGAAGA AACTTTGGGA CCTGCTGGAG 
AAGCCCAACT CATCGGTGGC CGCCAAGATC CTGGCCATCA TCTCCATCAT GTTCATTGTC 
CTCTCCACCA TTGCCCTGTC ACTCAACACA CTGCCTGAGC TACAGAGCCT GGACGAATTC 
GGCCAGAGCA CGGACAACCC GCAGCTGGCA CACGTGGAGG CTGTGTGCAT CGCGTGGTTC 
ACCATGGAGT ACTTGCTGAG GTTCCTGTCC TCGCCCAAGA AATGGAAGTT CTTTAAGGGC 
CCCCTCAACG CCATTGACTT ACTGGCCATC CTGCCCTACT ACGTCACCAT CTTCCTCACA 
GAATCCAACA AGAGCGTGCT GCAGTTCCAG AATGTGCGCC GTGTGGTCCA GATCTTCCGC 
ATCATGCGCA TCCTGCGCAT CCTGAAGTTG GCCCGCCACT CCACCGGTCT GCAGTCCTTG 
GGCTTCACGC TGCGCAGGAG CTACAACGAG CTGGGCTTGC TCATCCTCTT CCTCGCCATG 
GGCATCATGA TCTTCTCCAG CCTGGTCTTC TTTGCCGAGA AGGATGAGGA TGACACCAAG 
TTCAAAAGCA TCCCCGCCTC TTTCTGGTGG GCTACCATCA CCATGACGAC CGTTGGTTAC 
GGAGACATCT ACCCTAAGAC TCTCCTGGGG AAAATCGTGG GGGGCCTCTG TTGCATTGCC 
GGTGTCCTGG TGATTGCCCT CCCCATTCCA ATTATCGTCA ATAACTTCTC CGAGTTCTAC 
AAGGAGCAGA AGCGCCAGGA GAAAGCCATC AAGCGGAGAG AGGCTCTGGA GAGAGCCAAG 
AGGAACGGCA GCATCGTGTC CATGAACATG AAGGATGCCT TCGCCCGGAG CATCGAGATG 
ATGGACATCG TGGTGGAGAA AAATGGAGAG GGCGTGGCTA AGAAGGACAA GGTGCAGGAT 
AACCACCTGT CTCCCAACAA GTGGAAATGG ACCAAGAGGG CGCTCTCCGA GACCAGCTCG 
AGCAAGTCCT TTGAAACCAA GGAGCAGGGA TCTCCTGAGA AGGCCAGGTC CTCGTCTAGT 
CCGCAGCACT TGAACGTCCA GCAGCTGCAA GACATGTACA GCAAGATGGC CAAGACCCAG 
TCTCAACCCA TCCTCAACAC CAAGGAGATG GCGCCGCAGA GCCAGCCGCA GGAAGAACTG 
GAGATGGGCA GCATGCCCAG CCCCGTGGCC CCTCTGCCCA CGCGCACAGA GGGCGTCATC 
GACATGCGCA GCATGTCTAG CATCGACAGC TTCATCAGCT GCGCCACGGA CTTCCCTGAA 
GCCACCAGAT TCTCCCACAG TCCTCTGGCA TCCCTCTCCG GCAAGTCTGG GGGCAGCACA 
GCCCCGGAGG TGGGCTGGCG GGGGGCTCTG GGTGCCAGCG GCGGGAGACT CATGGAGACC 
AACCCCATCC CCGAGGCCAG CCGCTCTGGT TTCTTCGTGG AGAGCCCCCG GAGTTCCATG 
AAGACCCACA ACCCCATGAA GCTGCGAGCG CTCAAGGTTA ACTTCCTGGA GGGCGATCCC 
ACCCCGCTGC TACCGGCTCT GGGCTTGTAT CACGATCCTC TTAGGAACAG AGGAGGCGCA 
GCGGCTGCAG TCGCCGGACT GGAGTGTGCC TCCCTCTTAG ACAAGCCCGT GCTGAGCCCG 
GAGTCCTCCA TCTACACCAC AGCAAGTGCC AGGACGCCCC CTCGCTCCCC AGAGAAACAC 
ACAGCAATAG CGTTCAACTT CGAGGCGGGG GTCCACCAGT ACATAGACAC CGACACTGAT 
GACGAGGGTC AGCTGCTCTA CAGCGTGGAC TCCAGTCCTC CCAAGAGTCT CCACGGGAGT 
ACCAGCCCCA AGTTCAGCCT TGGAGCTAGA ACAGAAAAGA ACCACTTCGA GAGCTCCCCG 
TTGCCCACCT CCCCTAAGTT CTTAAGGCCG AACTGTGTCT ACGCCTCAGA AGGGTTACCT 
GGGAAAGGTC CCGGGGCCCA AGAGAAATGC AAGCTGGAGA ACCACACCTC CCCCGATGTG 
CACATGCTGC CTGGGGGAGG AGCACACGGG AGCACTCGGG ATCAGAGTAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032446.2
CDS	196..2769
Translation	MPAGMTKHGSRSTSSLPPEPMEIVRSKACSRRVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDCNTHDSLLQVCDDYSLEDNEYFFDRHPGAFTSILNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREAETLREREGEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPELQSLDEFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNMKDAFARSIEMMDIVVEKNGEGVAKKDKVQDNHLSPNKWKWTKRALSETSSSKSFETKEQGSPEKARSSSSPQHLNVQQLQDMYSKMAKTQSQPILNTKEMAPQSQPQEELEMGSMPSPVAPLPTRTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLASLSGKSGGSTAPEVGWRGALGASGGRLMETNPIPEASRSGFFVESPRSSMKTHNPMKLRALKVNFLEGDPTPLLPALGLYHDPLRNRGGAAAAVAGLECASLLDKPVLSPESSIYTTASARTPPRSPEKHTAIAFNFEAGVHQYIDTDTDDEGQLLYSVDSSPPKSLHGSTSPKFSLGARTEKNHFESSPLPTSPKFLRPNCVYASEGLPGKGPGAQEKCKLENHTSPDVHMLPGGGAHGSTRDQSI

Target ORF information:

RefSeq Version	NM_008420.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008420.4

ORF Insert Sequence:

ATGCCGGCGG GCATGACGAA GCATGGCTCG CGCTCCACCA GCTCGCTGCC GCCCGAGCCC 
ATGGAGATCG TGCGCAGCAA GGCGTGCTCG CGCCGGGTGC GCCTCAACGT CGGGGGCCTG 
GCGCACGAGG TGCTGTGGCG CACTCTGGAC CGCCTGCCTC GCACGCGGCT GGGCAAGCTC 
CGGGACTGCA ACACGCACGA CTCTCTGCTC CAGGTGTGCG ACGACTACAG CCTCGAGGAC 
AACGAGTACT TCTTCGACCG CCACCCTGGC GCCTTCACCT CTATTCTCAA TTTCTACCGC 
ACCGGCCGGC TGCACATGAT GGAGGAGATG TGCGCGCTGA GCTTCAGCCA GGAGCTGGAC 
TACTGGGGCA TCGATGAGAT CTACCTGGAG TCCTGCTGCC AGGCCCGCTA CCACCAAAAG 
AAGGAGCAGA TGAACGAGGA GCTGAAGCGG GAGGCTGAGA CGCTGCGGGA GCGGGAGGGC 
GAGGAGTTCG ACAACACGTG CTGTGCTGAG AAGAGGAAGA AACTTTGGGA CCTGCTGGAG 
AAGCCCAACT CATCGGTGGC CGCCAAGATC CTGGCCATCA TCTCCATCAT GTTCATTGTC 
CTCTCCACCA TTGCCCTGTC ACTCAACACA CTGCCTGAGC TACAGAGCCT GGACGAATTC 
GGCCAGAGCA CGGACAACCC GCAGCTGGCA CACGTGGAGG CTGTGTGCAT CGCGTGGTTC 
ACCATGGAGT ACTTGCTGAG GTTCCTGTCC TCGCCCAAGA AATGGAAGTT CTTTAAGGGC 
CCCCTCAACG CCATTGACTT ACTGGCCATC CTGCCCTACT ACGTCACCAT CTTCCTCACA 
GAATCCAACA AGAGCGTGCT GCAGTTCCAG AATGTGCGCC GTGTGGTCCA GATCTTCCGC 
ATCATGCGCA TCCTGCGCAT CCTGAAGTTG GCCCGCCACT CCACCGGTCT GCAGTCCTTG 
GGCTTCACGC TGCGCAGGAG CTACAACGAG CTGGGCTTGC TCATCCTCTT CCTCGCCATG 
GGCATCATGA TCTTCTCCAG CCTGGTCTTC TTTGCCGAGA AGGATGAGGA TGACACCAAG 
TTCAAAAGCA TCCCCGCCTC TTTCTGGTGG GCTACCATCA CCATGACGAC CGTTGGTTAC 
GGAGACATCT ACCCTAAGAC TCTCCTGGGG AAAATCGTGG GGGGCCTCTG TTGCATTGCC 
GGTGTCCTGG TGATTGCCCT CCCCATTCCA ATTATCGTCA ATAACTTCTC CGAGTTCTAC 
AAGGAGCAGA AGCGCCAGGA GAAAGCCATC AAGCGGAGAG AGGCTCTGGA GAGAGCCAAG 
AGGAACGGCA GCATCGTGTC CATGAACATG AAGGATGCCT TCGCCCGGAG CATCGAGATG 
ATGGACATCG TGGTGGAGAA AAATGGAGAG GGCGTGGCTA AGAAGGACAA GGTGCAGGAT 
AACCACCTGT CTCCCAACAA GTGGAAATGG ACCAAGAGGG CGCTCTCCGA GACCAGCTCG 
AGCAAGTCCT TTGAAACCAA GGAGCAGGGA TCTCCTGAGA AGGCCAGGTC CTCGTCTAGT 
CCGCAGCACT TGAACGTCCA GCAGCTGCAA GACATGTACA GCAAGATGGC CAAGACCCAG 
TCTCAACCCA TCCTCAACAC CAAGGAGATG GCGCCGCAGA GCCAGCCGCA GGAAGAACTG 
GAGATGGGCA GCATGCCCAG CCCCGTGGCC CCTCTGCCCA CGCGCACAGA GGGCGTCATC 
GACATGCGCA GCATGTCTAG CATCGACAGC TTCATCAGCT GCGCCACGGA CTTCCCTGAA 
GCCACCAGAT TCTCCCACAG TCCTCTGGCA TCCCTCTCCG GCAAGTCTGG GGGCAGCACA 
GCCCCGGAGG TGGGCTGGCG GGGGGCTCTG GGTGCCAGCG GCGGGAGACT CATGGAGACC 
AACCCCATCC CCGAGGCCAG CCGCTCTGGT TTCTTCGTGG AGAGCCCCCG GAGTTCCATG 
AAGACCCACA ACCCCATGAA GCTGCGAGCG CTCAAGGTTA ACTTCCTGGA GGGCGATCCC 
ACCCCGCTGC TACCGGCTCT GGGCTTGTAT CACGATCCTC TTAGGAACAG AGGAGGCGCA 
GCGGCTGCAG TCGCCGGACT GGAGTGTGCC TCCCTCTTAG ACAAGCCCGT GCTGAGCCCG 
GAGTCCTCCA TCTACACCAC AGCAAGTGCC AGGACGCCCC CTCGCTCCCC AGAGAAACAC 
ACAGCAATAG CGTTCAACTT CGAGGCGGGG GTCCACCAGT ACATAGACAC CGACACTGAT 
GACGAGGGTC AGCTGCTCTA CAGCGTGGAC TCCAGTCCTC CCAAGAGTCT CCACGGGAGT 
ACCAGCCCCA AGTTCAGCCT TGGAGCTAGA ACAGAAAAGA ACCACTTCGA GAGCTCCCCG 
TTGCCCACCT CCCCTAAGTT CTTAAGGCCG AACTGTGTCT ACGCCTCAGA AGGGTTACCT 
GGGAAAGGTC CCGGGGCCCA AGAGAAATGC AAGCTGGAGA ACCACACCTC CCCCGATGTG 
CACATGCTGC CTGGGGGAGG AGCACACGGG AGCACTCGGG ATCAGAGTAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnb1 ( XM_017315732.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171221.1 Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13702
Clone ID Related Accession (Same CDS sequence)	XM_017315732.1 , NM_008420.4 , XM_017315733.2 , XM_017315733.1 , XM_017315732.2
Accession Version	XM_017315732.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium voltage-gated channel subfamily B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCGG GCATGACGAA GCATGGCTCG CGCTCCACCA GCTCGCTGCC GCCCGAGCCC 
ATGGAGATCG TGCGCAGCAA GGCGTGCTCG CGCCGGGTGC GCCTCAACGT CGGGGGCCTG 
GCGCACGAGG TGCTGTGGCG CACTCTGGAC CGCCTGCCTC GCACGCGGCT GGGCAAGCTC 
CGGGACTGCA ACACGCACGA CTCTCTGCTC CAGGTGTGCG ACGACTACAG CCTCGAGGAC 
AACGAGTACT TCTTCGACCG CCACCCTGGC GCCTTCACCT CTATTCTCAA TTTCTACCGC 
ACCGGCCGGC TGCACATGAT GGAGGAGATG TGCGCGCTGA GCTTCAGCCA GGAGCTGGAC 
TACTGGGGCA TCGATGAGAT CTACCTGGAG TCCTGCTGCC AGGCCCGCTA CCACCAAAAG 
AAGGAGCAGA TGAACGAGGA GCTGAAGCGG GAGGCTGAGA CGCTGCGGGA GCGGGAGGGC 
GAGGAGTTCG ACAACACGTG CTGTGCTGAG AAGAGGAAGA AACTTTGGGA CCTGCTGGAG 
AAGCCCAACT CATCGGTGGC CGCCAAGATC CTGGCCATCA TCTCCATCAT GTTCATTGTC 
CTCTCCACCA TTGCCCTGTC ACTCAACACA CTGCCTGAGC TACAGAGCCT GGACGAATTC 
GGCCAGAGCA CGGACAACCC GCAGCTGGCA CACGTGGAGG CTGTGTGCAT CGCGTGGTTC 
ACCATGGAGT ACTTGCTGAG GTTCCTGTCC TCGCCCAAGA AATGGAAGTT CTTTAAGGGC 
CCCCTCAACG CCATTGACTT ACTGGCCATC CTGCCCTACT ACGTCACCAT CTTCCTCACA 
GAATCCAACA AGAGCGTGCT GCAGTTCCAG AATGTGCGCC GTGTGGTCCA GATCTTCCGC 
ATCATGCGCA TCCTGCGCAT CCTGAAGTTG GCCCGCCACT CCACCGGTCT GCAGTCCTTG 
GGCTTCACGC TGCGCAGGAG CTACAACGAG CTGGGCTTGC TCATCCTCTT CCTCGCCATG 
GGCATCATGA TCTTCTCCAG CCTGGTCTTC TTTGCCGAGA AGGATGAGGA TGACACCAAG 
TTCAAAAGCA TCCCCGCCTC TTTCTGGTGG GCTACCATCA CCATGACGAC CGTTGGTTAC 
GGAGACATCT ACCCTAAGAC TCTCCTGGGG AAAATCGTGG GGGGCCTCTG TTGCATTGCC 
GGTGTCCTGG TGATTGCCCT CCCCATTCCA ATTATCGTCA ATAACTTCTC CGAGTTCTAC 
AAGGAGCAGA AGCGCCAGGA GAAAGCCATC AAGCGGAGAG AGGCTCTGGA GAGAGCCAAG 
AGGAACGGCA GCATCGTGTC CATGAACATG AAGGATGCCT TCGCCCGGAG CATCGAGATG 
ATGGACATCG TGGTGGAGAA AAATGGAGAG GGCGTGGCTA AGAAGGACAA GGTGCAGGAT 
AACCACCTGT CTCCCAACAA GTGGAAATGG ACCAAGAGGG CGCTCTCCGA GACCAGCTCG 
AGCAAGTCCT TTGAAACCAA GGAGCAGGGA TCTCCTGAGA AGGCCAGGTC CTCGTCTAGT 
CCGCAGCACT TGAACGTCCA GCAGCTGCAA GACATGTACA GCAAGATGGC CAAGACCCAG 
TCTCAACCCA TCCTCAACAC CAAGGAGATG GCGCCGCAGA GCCAGCCGCA GGAAGAACTG 
GAGATGGGCA GCATGCCCAG CCCCGTGGCC CCTCTGCCCA CGCGCACAGA GGGCGTCATC 
GACATGCGCA GCATGTCTAG CATCGACAGC TTCATCAGCT GCGCCACGGA CTTCCCTGAA 
GCCACCAGAT TCTCCCACAG TCCTCTGGCA TCCCTCTCCG GCAAGTCTGG GGGCAGCACA 
GCCCCGGAGG TGGGCTGGCG GGGGGCTCTG GGTGCCAGCG GCGGGAGACT CATGGAGACC 
AACCCCATCC CCGAGGCCAG CCGCTCTGGT TTCTTCGTGG AGAGCCCCCG GAGTTCCATG 
AAGACCCACA ACCCCATGAA GCTGCGAGCG CTCAAGGTTA ACTTCCTGGA GGGCGATCCC 
ACCCCGCTGC TACCGGCTCT GGGCTTGTAT CACGATCCTC TTAGGAACAG AGGAGGCGCA 
GCGGCTGCAG TCGCCGGACT GGAGTGTGCC TCCCTCTTAG ACAAGCCCGT GCTGAGCCCG 
GAGTCCTCCA TCTACACCAC AGCAAGTGCC AGGACGCCCC CTCGCTCCCC AGAGAAACAC 
ACAGCAATAG CGTTCAACTT CGAGGCGGGG GTCCACCAGT ACATAGACAC CGACACTGAT 
GACGAGGGTC AGCTGCTCTA CAGCGTGGAC TCCAGTCCTC CCAAGAGTCT CCACGGGAGT 
ACCAGCCCCA AGTTCAGCCT TGGAGCTAGA ACAGAAAAGA ACCACTTCGA GAGCTCCCCG 
TTGCCCACCT CCCCTAAGTT CTTAAGGCCG AACTGTGTCT ACGCCTCAGA AGGGTTACCT 
GGGAAAGGTC CCGGGGCCCA AGAGAAATGC AAGCTGGAGA ACCACACCTC CCCCGATGTG 
CACATGCTGC CTGGGGGAGG AGCACACGGG AGCACTCGGG ATCAGAGTAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171221.1
CDS	334..2907
Translation	MPAGMTKHGSRSTSSLPPEPMEIVRSKACSRRVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDCNTHDSLLQVCDDYSLEDNEYFFDRHPGAFTSILNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREAETLREREGEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPELQSLDEFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNMKDAFARSIEMMDIVVEKNGEGVAKKDKVQDNHLSPNKWKWTKRALSETSSSKSFETKEQGSPEKARSSSSPQHLNVQQLQDMYSKMAKTQSQPILNTKEMAPQSQPQEELEMGSMPSPVAPLPTRTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLASLSGKSGGSTAPEVGWRGALGASGGRLMETNPIPEASRSGFFVESPRSSMKTHNPMKLRALKVNFLEGDPTPLLPALGLYHDPLRNRGGAAAAVAGLECASLLDKPVLSPESSIYTTASARTPPRSPEKHTAIAFNFEAGVHQYIDTDTDDEGQLLYSVDSSPPKSLHGSTSPKFSLGARTEKNHFESSPLPTSPKFLRPNCVYASEGLPGKGPGAQEKCKLENHTSPDVHMLPGGGAHGSTRDQSI

Target ORF information:

RefSeq Version	XM_017315732.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315732.1

ORF Insert Sequence:

ATGCCGGCGG GCATGACGAA GCATGGCTCG CGCTCCACCA GCTCGCTGCC GCCCGAGCCC 
ATGGAGATCG TGCGCAGCAA GGCGTGCTCG CGCCGGGTGC GCCTCAACGT CGGGGGCCTG 
GCGCACGAGG TGCTGTGGCG CACTCTGGAC CGCCTGCCTC GCACGCGGCT GGGCAAGCTC 
CGGGACTGCA ACACGCACGA CTCTCTGCTC CAGGTGTGCG ACGACTACAG CCTCGAGGAC 
AACGAGTACT TCTTCGACCG CCACCCTGGC GCCTTCACCT CTATTCTCAA TTTCTACCGC 
ACCGGCCGGC TGCACATGAT GGAGGAGATG TGCGCGCTGA GCTTCAGCCA GGAGCTGGAC 
TACTGGGGCA TCGATGAGAT CTACCTGGAG TCCTGCTGCC AGGCCCGCTA CCACCAAAAG 
AAGGAGCAGA TGAACGAGGA GCTGAAGCGG GAGGCTGAGA CGCTGCGGGA GCGGGAGGGC 
GAGGAGTTCG ACAACACGTG CTGTGCTGAG AAGAGGAAGA AACTTTGGGA CCTGCTGGAG 
AAGCCCAACT CATCGGTGGC CGCCAAGATC CTGGCCATCA TCTCCATCAT GTTCATTGTC 
CTCTCCACCA TTGCCCTGTC ACTCAACACA CTGCCTGAGC TACAGAGCCT GGACGAATTC 
GGCCAGAGCA CGGACAACCC GCAGCTGGCA CACGTGGAGG CTGTGTGCAT CGCGTGGTTC 
ACCATGGAGT ACTTGCTGAG GTTCCTGTCC TCGCCCAAGA AATGGAAGTT CTTTAAGGGC 
CCCCTCAACG CCATTGACTT ACTGGCCATC CTGCCCTACT ACGTCACCAT CTTCCTCACA 
GAATCCAACA AGAGCGTGCT GCAGTTCCAG AATGTGCGCC GTGTGGTCCA GATCTTCCGC 
ATCATGCGCA TCCTGCGCAT CCTGAAGTTG GCCCGCCACT CCACCGGTCT GCAGTCCTTG 
GGCTTCACGC TGCGCAGGAG CTACAACGAG CTGGGCTTGC TCATCCTCTT CCTCGCCATG 
GGCATCATGA TCTTCTCCAG CCTGGTCTTC TTTGCCGAGA AGGATGAGGA TGACACCAAG 
TTCAAAAGCA TCCCCGCCTC TTTCTGGTGG GCTACCATCA CCATGACGAC CGTTGGTTAC 
GGAGACATCT ACCCTAAGAC TCTCCTGGGG AAAATCGTGG GGGGCCTCTG TTGCATTGCC 
GGTGTCCTGG TGATTGCCCT CCCCATTCCA ATTATCGTCA ATAACTTCTC CGAGTTCTAC 
AAGGAGCAGA AGCGCCAGGA GAAAGCCATC AAGCGGAGAG AGGCTCTGGA GAGAGCCAAG 
AGGAACGGCA GCATCGTGTC CATGAACATG AAGGATGCCT TCGCCCGGAG CATCGAGATG 
ATGGACATCG TGGTGGAGAA AAATGGAGAG GGCGTGGCTA AGAAGGACAA GGTGCAGGAT 
AACCACCTGT CTCCCAACAA GTGGAAATGG ACCAAGAGGG CGCTCTCCGA GACCAGCTCG 
AGCAAGTCCT TTGAAACCAA GGAGCAGGGA TCTCCTGAGA AGGCCAGGTC CTCGTCTAGT 
CCGCAGCACT TGAACGTCCA GCAGCTGCAA GACATGTACA GCAAGATGGC CAAGACCCAG 
TCTCAACCCA TCCTCAACAC CAAGGAGATG GCGCCGCAGA GCCAGCCGCA GGAAGAACTG 
GAGATGGGCA GCATGCCCAG CCCCGTGGCC CCTCTGCCCA CGCGCACAGA GGGCGTCATC 
GACATGCGCA GCATGTCTAG CATCGACAGC TTCATCAGCT GCGCCACGGA CTTCCCTGAA 
GCCACCAGAT TCTCCCACAG TCCTCTGGCA TCCCTCTCCG GCAAGTCTGG GGGCAGCACA 
GCCCCGGAGG TGGGCTGGCG GGGGGCTCTG GGTGCCAGCG GCGGGAGACT CATGGAGACC 
AACCCCATCC CCGAGGCCAG CCGCTCTGGT TTCTTCGTGG AGAGCCCCCG GAGTTCCATG 
AAGACCCACA ACCCCATGAA GCTGCGAGCG CTCAAGGTTA ACTTCCTGGA GGGCGATCCC 
ACCCCGCTGC TACCGGCTCT GGGCTTGTAT CACGATCCTC TTAGGAACAG AGGAGGCGCA 
GCGGCTGCAG TCGCCGGACT GGAGTGTGCC TCCCTCTTAG ACAAGCCCGT GCTGAGCCCG 
GAGTCCTCCA TCTACACCAC AGCAAGTGCC AGGACGCCCC CTCGCTCCCC AGAGAAACAC 
ACAGCAATAG CGTTCAACTT CGAGGCGGGG GTCCACCAGT ACATAGACAC CGACACTGAT 
GACGAGGGTC AGCTGCTCTA CAGCGTGGAC TCCAGTCCTC CCAAGAGTCT CCACGGGAGT 
ACCAGCCCCA AGTTCAGCCT TGGAGCTAGA ACAGAAAAGA ACCACTTCGA GAGCTCCCCG 
TTGCCCACCT CCCCTAAGTT CTTAAGGCCG AACTGTGTCT ACGCCTCAGA AGGGTTACCT 
GGGAAAGGTC CCGGGGCCCA AGAGAAATGC AAGCTGGAGA ACCACACCTC CCCCGATGTG 
CACATGCTGC CTGGGGGAGG AGCACACGGG AGCACTCGGG ATCAGAGTAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnb1 ( XM_017315733.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171222.1 Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X2, mRNA.

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CloneID	OMu13702
Clone ID Related Accession (Same CDS sequence)	XM_017315732.1 , NM_008420.4 , XM_017315733.2 , XM_017315733.1 , XM_017315732.2
Accession Version	XM_017315733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium voltage-gated channel subfamily B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCGG GCATGACGAA GCATGGCTCG CGCTCCACCA GCTCGCTGCC GCCCGAGCCC 
ATGGAGATCG TGCGCAGCAA GGCGTGCTCG CGCCGGGTGC GCCTCAACGT CGGGGGCCTG 
GCGCACGAGG TGCTGTGGCG CACTCTGGAC CGCCTGCCTC GCACGCGGCT GGGCAAGCTC 
CGGGACTGCA ACACGCACGA CTCTCTGCTC CAGGTGTGCG ACGACTACAG CCTCGAGGAC 
AACGAGTACT TCTTCGACCG CCACCCTGGC GCCTTCACCT CTATTCTCAA TTTCTACCGC 
ACCGGCCGGC TGCACATGAT GGAGGAGATG TGCGCGCTGA GCTTCAGCCA GGAGCTGGAC 
TACTGGGGCA TCGATGAGAT CTACCTGGAG TCCTGCTGCC AGGCCCGCTA CCACCAAAAG 
AAGGAGCAGA TGAACGAGGA GCTGAAGCGG GAGGCTGAGA CGCTGCGGGA GCGGGAGGGC 
GAGGAGTTCG ACAACACGTG CTGTGCTGAG AAGAGGAAGA AACTTTGGGA CCTGCTGGAG 
AAGCCCAACT CATCGGTGGC CGCCAAGATC CTGGCCATCA TCTCCATCAT GTTCATTGTC 
CTCTCCACCA TTGCCCTGTC ACTCAACACA CTGCCTGAGC TACAGAGCCT GGACGAATTC 
GGCCAGAGCA CGGACAACCC GCAGCTGGCA CACGTGGAGG CTGTGTGCAT CGCGTGGTTC 
ACCATGGAGT ACTTGCTGAG GTTCCTGTCC TCGCCCAAGA AATGGAAGTT CTTTAAGGGC 
CCCCTCAACG CCATTGACTT ACTGGCCATC CTGCCCTACT ACGTCACCAT CTTCCTCACA 
GAATCCAACA AGAGCGTGCT GCAGTTCCAG AATGTGCGCC GTGTGGTCCA GATCTTCCGC 
ATCATGCGCA TCCTGCGCAT CCTGAAGTTG GCCCGCCACT CCACCGGTCT GCAGTCCTTG 
GGCTTCACGC TGCGCAGGAG CTACAACGAG CTGGGCTTGC TCATCCTCTT CCTCGCCATG 
GGCATCATGA TCTTCTCCAG CCTGGTCTTC TTTGCCGAGA AGGATGAGGA TGACACCAAG 
TTCAAAAGCA TCCCCGCCTC TTTCTGGTGG GCTACCATCA CCATGACGAC CGTTGGTTAC 
GGAGACATCT ACCCTAAGAC TCTCCTGGGG AAAATCGTGG GGGGCCTCTG TTGCATTGCC 
GGTGTCCTGG TGATTGCCCT CCCCATTCCA ATTATCGTCA ATAACTTCTC CGAGTTCTAC 
AAGGAGCAGA AGCGCCAGGA GAAAGCCATC AAGCGGAGAG AGGCTCTGGA GAGAGCCAAG 
AGGAACGGCA GCATCGTGTC CATGAACATG AAGGATGCCT TCGCCCGGAG CATCGAGATG 
ATGGACATCG TGGTGGAGAA AAATGGAGAG GGCGTGGCTA AGAAGGACAA GGTGCAGGAT 
AACCACCTGT CTCCCAACAA GTGGAAATGG ACCAAGAGGG CGCTCTCCGA GACCAGCTCG 
AGCAAGTCCT TTGAAACCAA GGAGCAGGGA TCTCCTGAGA AGGCCAGGTC CTCGTCTAGT 
CCGCAGCACT TGAACGTCCA GCAGCTGCAA GACATGTACA GCAAGATGGC CAAGACCCAG 
TCTCAACCCA TCCTCAACAC CAAGGAGATG GCGCCGCAGA GCCAGCCGCA GGAAGAACTG 
GAGATGGGCA GCATGCCCAG CCCCGTGGCC CCTCTGCCCA CGCGCACAGA GGGCGTCATC 
GACATGCGCA GCATGTCTAG CATCGACAGC TTCATCAGCT GCGCCACGGA CTTCCCTGAA 
GCCACCAGAT TCTCCCACAG TCCTCTGGCA TCCCTCTCCG GCAAGTCTGG GGGCAGCACA 
GCCCCGGAGG TGGGCTGGCG GGGGGCTCTG GGTGCCAGCG GCGGGAGACT CATGGAGACC 
AACCCCATCC CCGAGGCCAG CCGCTCTGGT TTCTTCGTGG AGAGCCCCCG GAGTTCCATG 
AAGACCCACA ACCCCATGAA GCTGCGAGCG CTCAAGGTTA ACTTCCTGGA GGGCGATCCC 
ACCCCGCTGC TACCGGCTCT GGGCTTGTAT CACGATCCTC TTAGGAACAG AGGAGGCGCA 
GCGGCTGCAG TCGCCGGACT GGAGTGTGCC TCCCTCTTAG ACAAGCCCGT GCTGAGCCCG 
GAGTCCTCCA TCTACACCAC AGCAAGTGCC AGGACGCCCC CTCGCTCCCC AGAGAAACAC 
ACAGCAATAG CGTTCAACTT CGAGGCGGGG GTCCACCAGT ACATAGACAC CGACACTGAT 
GACGAGGGTC AGCTGCTCTA CAGCGTGGAC TCCAGTCCTC CCAAGAGTCT CCACGGGAGT 
ACCAGCCCCA AGTTCAGCCT TGGAGCTAGA ACAGAAAAGA ACCACTTCGA GAGCTCCCCG 
TTGCCCACCT CCCCTAAGTT CTTAAGGCCG AACTGTGTCT ACGCCTCAGA AGGGTTACCT 
GGGAAAGGTC CCGGGGCCCA AGAGAAATGC AAGCTGGAGA ACCACACCTC CCCCGATGTG 
CACATGCTGC CTGGGGGAGG AGCACACGGG AGCACTCGGG ATCAGAGTAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171222.1
CDS	368..2941
Translation	MPAGMTKHGSRSTSSLPPEPMEIVRSKACSRRVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDCNTHDSLLQVCDDYSLEDNEYFFDRHPGAFTSILNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREAETLREREGEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPELQSLDEFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNMKDAFARSIEMMDIVVEKNGEGVAKKDKVQDNHLSPNKWKWTKRALSETSSSKSFETKEQGSPEKARSSSSPQHLNVQQLQDMYSKMAKTQSQPILNTKEMAPQSQPQEELEMGSMPSPVAPLPTRTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLASLSGKSGGSTAPEVGWRGALGASGGRLMETNPIPEASRSGFFVESPRSSMKTHNPMKLRALKVNFLEGDPTPLLPALGLYHDPLRNRGGAAAAVAGLECASLLDKPVLSPESSIYTTASARTPPRSPEKHTAIAFNFEAGVHQYIDTDTDDEGQLLYSVDSSPPKSLHGSTSPKFSLGARTEKNHFESSPLPTSPKFLRPNCVYASEGLPGKGPGAQEKCKLENHTSPDVHMLPGGGAHGSTRDQSI

Target ORF information:

RefSeq Version	XM_017315733.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315733.2

ORF Insert Sequence:

ATGCCGGCGG GCATGACGAA GCATGGCTCG CGCTCCACCA GCTCGCTGCC GCCCGAGCCC 
ATGGAGATCG TGCGCAGCAA GGCGTGCTCG CGCCGGGTGC GCCTCAACGT CGGGGGCCTG 
GCGCACGAGG TGCTGTGGCG CACTCTGGAC CGCCTGCCTC GCACGCGGCT GGGCAAGCTC 
CGGGACTGCA ACACGCACGA CTCTCTGCTC CAGGTGTGCG ACGACTACAG CCTCGAGGAC 
AACGAGTACT TCTTCGACCG CCACCCTGGC GCCTTCACCT CTATTCTCAA TTTCTACCGC 
ACCGGCCGGC TGCACATGAT GGAGGAGATG TGCGCGCTGA GCTTCAGCCA GGAGCTGGAC 
TACTGGGGCA TCGATGAGAT CTACCTGGAG TCCTGCTGCC AGGCCCGCTA CCACCAAAAG 
AAGGAGCAGA TGAACGAGGA GCTGAAGCGG GAGGCTGAGA CGCTGCGGGA GCGGGAGGGC 
GAGGAGTTCG ACAACACGTG CTGTGCTGAG AAGAGGAAGA AACTTTGGGA CCTGCTGGAG 
AAGCCCAACT CATCGGTGGC CGCCAAGATC CTGGCCATCA TCTCCATCAT GTTCATTGTC 
CTCTCCACCA TTGCCCTGTC ACTCAACACA CTGCCTGAGC TACAGAGCCT GGACGAATTC 
GGCCAGAGCA CGGACAACCC GCAGCTGGCA CACGTGGAGG CTGTGTGCAT CGCGTGGTTC 
ACCATGGAGT ACTTGCTGAG GTTCCTGTCC TCGCCCAAGA AATGGAAGTT CTTTAAGGGC 
CCCCTCAACG CCATTGACTT ACTGGCCATC CTGCCCTACT ACGTCACCAT CTTCCTCACA 
GAATCCAACA AGAGCGTGCT GCAGTTCCAG AATGTGCGCC GTGTGGTCCA GATCTTCCGC 
ATCATGCGCA TCCTGCGCAT CCTGAAGTTG GCCCGCCACT CCACCGGTCT GCAGTCCTTG 
GGCTTCACGC TGCGCAGGAG CTACAACGAG CTGGGCTTGC TCATCCTCTT CCTCGCCATG 
GGCATCATGA TCTTCTCCAG CCTGGTCTTC TTTGCCGAGA AGGATGAGGA TGACACCAAG 
TTCAAAAGCA TCCCCGCCTC TTTCTGGTGG GCTACCATCA CCATGACGAC CGTTGGTTAC 
GGAGACATCT ACCCTAAGAC TCTCCTGGGG AAAATCGTGG GGGGCCTCTG TTGCATTGCC 
GGTGTCCTGG TGATTGCCCT CCCCATTCCA ATTATCGTCA ATAACTTCTC CGAGTTCTAC 
AAGGAGCAGA AGCGCCAGGA GAAAGCCATC AAGCGGAGAG AGGCTCTGGA GAGAGCCAAG 
AGGAACGGCA GCATCGTGTC CATGAACATG AAGGATGCCT TCGCCCGGAG CATCGAGATG 
ATGGACATCG TGGTGGAGAA AAATGGAGAG GGCGTGGCTA AGAAGGACAA GGTGCAGGAT 
AACCACCTGT CTCCCAACAA GTGGAAATGG ACCAAGAGGG CGCTCTCCGA GACCAGCTCG 
AGCAAGTCCT TTGAAACCAA GGAGCAGGGA TCTCCTGAGA AGGCCAGGTC CTCGTCTAGT 
CCGCAGCACT TGAACGTCCA GCAGCTGCAA GACATGTACA GCAAGATGGC CAAGACCCAG 
TCTCAACCCA TCCTCAACAC CAAGGAGATG GCGCCGCAGA GCCAGCCGCA GGAAGAACTG 
GAGATGGGCA GCATGCCCAG CCCCGTGGCC CCTCTGCCCA CGCGCACAGA GGGCGTCATC 
GACATGCGCA GCATGTCTAG CATCGACAGC TTCATCAGCT GCGCCACGGA CTTCCCTGAA 
GCCACCAGAT TCTCCCACAG TCCTCTGGCA TCCCTCTCCG GCAAGTCTGG GGGCAGCACA 
GCCCCGGAGG TGGGCTGGCG GGGGGCTCTG GGTGCCAGCG GCGGGAGACT CATGGAGACC 
AACCCCATCC CCGAGGCCAG CCGCTCTGGT TTCTTCGTGG AGAGCCCCCG GAGTTCCATG 
AAGACCCACA ACCCCATGAA GCTGCGAGCG CTCAAGGTTA ACTTCCTGGA GGGCGATCCC 
ACCCCGCTGC TACCGGCTCT GGGCTTGTAT CACGATCCTC TTAGGAACAG AGGAGGCGCA 
GCGGCTGCAG TCGCCGGACT GGAGTGTGCC TCCCTCTTAG ACAAGCCCGT GCTGAGCCCG 
GAGTCCTCCA TCTACACCAC AGCAAGTGCC AGGACGCCCC CTCGCTCCCC AGAGAAACAC 
ACAGCAATAG CGTTCAACTT CGAGGCGGGG GTCCACCAGT ACATAGACAC CGACACTGAT 
GACGAGGGTC AGCTGCTCTA CAGCGTGGAC TCCAGTCCTC CCAAGAGTCT CCACGGGAGT 
ACCAGCCCCA AGTTCAGCCT TGGAGCTAGA ACAGAAAAGA ACCACTTCGA GAGCTCCCCG 
TTGCCCACCT CCCCTAAGTT CTTAAGGCCG AACTGTGTCT ACGCCTCAGA AGGGTTACCT 
GGGAAAGGTC CCGGGGCCCA AGAGAAATGC AAGCTGGAGA ACCACACCTC CCCCGATGTG 
CACATGCTGC CTGGGGGAGG AGCACACGGG AGCACTCGGG ATCAGAGTAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnb1 ( XM_017315733.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171222.1 Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu13702
Clone ID Related Accession (Same CDS sequence)	XM_017315732.1 , NM_008420.4 , XM_017315733.2 , XM_017315733.1 , XM_017315732.2
Accession Version	XM_017315733.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium voltage-gated channel subfamily B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCGG GCATGACGAA GCATGGCTCG CGCTCCACCA GCTCGCTGCC GCCCGAGCCC 
ATGGAGATCG TGCGCAGCAA GGCGTGCTCG CGCCGGGTGC GCCTCAACGT CGGGGGCCTG 
GCGCACGAGG TGCTGTGGCG CACTCTGGAC CGCCTGCCTC GCACGCGGCT GGGCAAGCTC 
CGGGACTGCA ACACGCACGA CTCTCTGCTC CAGGTGTGCG ACGACTACAG CCTCGAGGAC 
AACGAGTACT TCTTCGACCG CCACCCTGGC GCCTTCACCT CTATTCTCAA TTTCTACCGC 
ACCGGCCGGC TGCACATGAT GGAGGAGATG TGCGCGCTGA GCTTCAGCCA GGAGCTGGAC 
TACTGGGGCA TCGATGAGAT CTACCTGGAG TCCTGCTGCC AGGCCCGCTA CCACCAAAAG 
AAGGAGCAGA TGAACGAGGA GCTGAAGCGG GAGGCTGAGA CGCTGCGGGA GCGGGAGGGC 
GAGGAGTTCG ACAACACGTG CTGTGCTGAG AAGAGGAAGA AACTTTGGGA CCTGCTGGAG 
AAGCCCAACT CATCGGTGGC CGCCAAGATC CTGGCCATCA TCTCCATCAT GTTCATTGTC 
CTCTCCACCA TTGCCCTGTC ACTCAACACA CTGCCTGAGC TACAGAGCCT GGACGAATTC 
GGCCAGAGCA CGGACAACCC GCAGCTGGCA CACGTGGAGG CTGTGTGCAT CGCGTGGTTC 
ACCATGGAGT ACTTGCTGAG GTTCCTGTCC TCGCCCAAGA AATGGAAGTT CTTTAAGGGC 
CCCCTCAACG CCATTGACTT ACTGGCCATC CTGCCCTACT ACGTCACCAT CTTCCTCACA 
GAATCCAACA AGAGCGTGCT GCAGTTCCAG AATGTGCGCC GTGTGGTCCA GATCTTCCGC 
ATCATGCGCA TCCTGCGCAT CCTGAAGTTG GCCCGCCACT CCACCGGTCT GCAGTCCTTG 
GGCTTCACGC TGCGCAGGAG CTACAACGAG CTGGGCTTGC TCATCCTCTT CCTCGCCATG 
GGCATCATGA TCTTCTCCAG CCTGGTCTTC TTTGCCGAGA AGGATGAGGA TGACACCAAG 
TTCAAAAGCA TCCCCGCCTC TTTCTGGTGG GCTACCATCA CCATGACGAC CGTTGGTTAC 
GGAGACATCT ACCCTAAGAC TCTCCTGGGG AAAATCGTGG GGGGCCTCTG TTGCATTGCC 
GGTGTCCTGG TGATTGCCCT CCCCATTCCA ATTATCGTCA ATAACTTCTC CGAGTTCTAC 
AAGGAGCAGA AGCGCCAGGA GAAAGCCATC AAGCGGAGAG AGGCTCTGGA GAGAGCCAAG 
AGGAACGGCA GCATCGTGTC CATGAACATG AAGGATGCCT TCGCCCGGAG CATCGAGATG 
ATGGACATCG TGGTGGAGAA AAATGGAGAG GGCGTGGCTA AGAAGGACAA GGTGCAGGAT 
AACCACCTGT CTCCCAACAA GTGGAAATGG ACCAAGAGGG CGCTCTCCGA GACCAGCTCG 
AGCAAGTCCT TTGAAACCAA GGAGCAGGGA TCTCCTGAGA AGGCCAGGTC CTCGTCTAGT 
CCGCAGCACT TGAACGTCCA GCAGCTGCAA GACATGTACA GCAAGATGGC CAAGACCCAG 
TCTCAACCCA TCCTCAACAC CAAGGAGATG GCGCCGCAGA GCCAGCCGCA GGAAGAACTG 
GAGATGGGCA GCATGCCCAG CCCCGTGGCC CCTCTGCCCA CGCGCACAGA GGGCGTCATC 
GACATGCGCA GCATGTCTAG CATCGACAGC TTCATCAGCT GCGCCACGGA CTTCCCTGAA 
GCCACCAGAT TCTCCCACAG TCCTCTGGCA TCCCTCTCCG GCAAGTCTGG GGGCAGCACA 
GCCCCGGAGG TGGGCTGGCG GGGGGCTCTG GGTGCCAGCG GCGGGAGACT CATGGAGACC 
AACCCCATCC CCGAGGCCAG CCGCTCTGGT TTCTTCGTGG AGAGCCCCCG GAGTTCCATG 
AAGACCCACA ACCCCATGAA GCTGCGAGCG CTCAAGGTTA ACTTCCTGGA GGGCGATCCC 
ACCCCGCTGC TACCGGCTCT GGGCTTGTAT CACGATCCTC TTAGGAACAG AGGAGGCGCA 
GCGGCTGCAG TCGCCGGACT GGAGTGTGCC TCCCTCTTAG ACAAGCCCGT GCTGAGCCCG 
GAGTCCTCCA TCTACACCAC AGCAAGTGCC AGGACGCCCC CTCGCTCCCC AGAGAAACAC 
ACAGCAATAG CGTTCAACTT CGAGGCGGGG GTCCACCAGT ACATAGACAC CGACACTGAT 
GACGAGGGTC AGCTGCTCTA CAGCGTGGAC TCCAGTCCTC CCAAGAGTCT CCACGGGAGT 
ACCAGCCCCA AGTTCAGCCT TGGAGCTAGA ACAGAAAAGA ACCACTTCGA GAGCTCCCCG 
TTGCCCACCT CCCCTAAGTT CTTAAGGCCG AACTGTGTCT ACGCCTCAGA AGGGTTACCT 
GGGAAAGGTC CCGGGGCCCA AGAGAAATGC AAGCTGGAGA ACCACACCTC CCCCGATGTG 
CACATGCTGC CTGGGGGAGG AGCACACGGG AGCACTCGGG ATCAGAGTAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171222.1
CDS	245..2818
Translation	MPAGMTKHGSRSTSSLPPEPMEIVRSKACSRRVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDCNTHDSLLQVCDDYSLEDNEYFFDRHPGAFTSILNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREAETLREREGEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPELQSLDEFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNMKDAFARSIEMMDIVVEKNGEGVAKKDKVQDNHLSPNKWKWTKRALSETSSSKSFETKEQGSPEKARSSSSPQHLNVQQLQDMYSKMAKTQSQPILNTKEMAPQSQPQEELEMGSMPSPVAPLPTRTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLASLSGKSGGSTAPEVGWRGALGASGGRLMETNPIPEASRSGFFVESPRSSMKTHNPMKLRALKVNFLEGDPTPLLPALGLYHDPLRNRGGAAAAVAGLECASLLDKPVLSPESSIYTTASARTPPRSPEKHTAIAFNFEAGVHQYIDTDTDDEGQLLYSVDSSPPKSLHGSTSPKFSLGARTEKNHFESSPLPTSPKFLRPNCVYASEGLPGKGPGAQEKCKLENHTSPDVHMLPGGGAHGSTRDQSI

Target ORF information:

RefSeq Version	XM_017315733.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315733.1

ORF Insert Sequence:

ATGCCGGCGG GCATGACGAA GCATGGCTCG CGCTCCACCA GCTCGCTGCC GCCCGAGCCC 
ATGGAGATCG TGCGCAGCAA GGCGTGCTCG CGCCGGGTGC GCCTCAACGT CGGGGGCCTG 
GCGCACGAGG TGCTGTGGCG CACTCTGGAC CGCCTGCCTC GCACGCGGCT GGGCAAGCTC 
CGGGACTGCA ACACGCACGA CTCTCTGCTC CAGGTGTGCG ACGACTACAG CCTCGAGGAC 
AACGAGTACT TCTTCGACCG CCACCCTGGC GCCTTCACCT CTATTCTCAA TTTCTACCGC 
ACCGGCCGGC TGCACATGAT GGAGGAGATG TGCGCGCTGA GCTTCAGCCA GGAGCTGGAC 
TACTGGGGCA TCGATGAGAT CTACCTGGAG TCCTGCTGCC AGGCCCGCTA CCACCAAAAG 
AAGGAGCAGA TGAACGAGGA GCTGAAGCGG GAGGCTGAGA CGCTGCGGGA GCGGGAGGGC 
GAGGAGTTCG ACAACACGTG CTGTGCTGAG AAGAGGAAGA AACTTTGGGA CCTGCTGGAG 
AAGCCCAACT CATCGGTGGC CGCCAAGATC CTGGCCATCA TCTCCATCAT GTTCATTGTC 
CTCTCCACCA TTGCCCTGTC ACTCAACACA CTGCCTGAGC TACAGAGCCT GGACGAATTC 
GGCCAGAGCA CGGACAACCC GCAGCTGGCA CACGTGGAGG CTGTGTGCAT CGCGTGGTTC 
ACCATGGAGT ACTTGCTGAG GTTCCTGTCC TCGCCCAAGA AATGGAAGTT CTTTAAGGGC 
CCCCTCAACG CCATTGACTT ACTGGCCATC CTGCCCTACT ACGTCACCAT CTTCCTCACA 
GAATCCAACA AGAGCGTGCT GCAGTTCCAG AATGTGCGCC GTGTGGTCCA GATCTTCCGC 
ATCATGCGCA TCCTGCGCAT CCTGAAGTTG GCCCGCCACT CCACCGGTCT GCAGTCCTTG 
GGCTTCACGC TGCGCAGGAG CTACAACGAG CTGGGCTTGC TCATCCTCTT CCTCGCCATG 
GGCATCATGA TCTTCTCCAG CCTGGTCTTC TTTGCCGAGA AGGATGAGGA TGACACCAAG 
TTCAAAAGCA TCCCCGCCTC TTTCTGGTGG GCTACCATCA CCATGACGAC CGTTGGTTAC 
GGAGACATCT ACCCTAAGAC TCTCCTGGGG AAAATCGTGG GGGGCCTCTG TTGCATTGCC 
GGTGTCCTGG TGATTGCCCT CCCCATTCCA ATTATCGTCA ATAACTTCTC CGAGTTCTAC 
AAGGAGCAGA AGCGCCAGGA GAAAGCCATC AAGCGGAGAG AGGCTCTGGA GAGAGCCAAG 
AGGAACGGCA GCATCGTGTC CATGAACATG AAGGATGCCT TCGCCCGGAG CATCGAGATG 
ATGGACATCG TGGTGGAGAA AAATGGAGAG GGCGTGGCTA AGAAGGACAA GGTGCAGGAT 
AACCACCTGT CTCCCAACAA GTGGAAATGG ACCAAGAGGG CGCTCTCCGA GACCAGCTCG 
AGCAAGTCCT TTGAAACCAA GGAGCAGGGA TCTCCTGAGA AGGCCAGGTC CTCGTCTAGT 
CCGCAGCACT TGAACGTCCA GCAGCTGCAA GACATGTACA GCAAGATGGC CAAGACCCAG 
TCTCAACCCA TCCTCAACAC CAAGGAGATG GCGCCGCAGA GCCAGCCGCA GGAAGAACTG 
GAGATGGGCA GCATGCCCAG CCCCGTGGCC CCTCTGCCCA CGCGCACAGA GGGCGTCATC 
GACATGCGCA GCATGTCTAG CATCGACAGC TTCATCAGCT GCGCCACGGA CTTCCCTGAA 
GCCACCAGAT TCTCCCACAG TCCTCTGGCA TCCCTCTCCG GCAAGTCTGG GGGCAGCACA 
GCCCCGGAGG TGGGCTGGCG GGGGGCTCTG GGTGCCAGCG GCGGGAGACT CATGGAGACC 
AACCCCATCC CCGAGGCCAG CCGCTCTGGT TTCTTCGTGG AGAGCCCCCG GAGTTCCATG 
AAGACCCACA ACCCCATGAA GCTGCGAGCG CTCAAGGTTA ACTTCCTGGA GGGCGATCCC 
ACCCCGCTGC TACCGGCTCT GGGCTTGTAT CACGATCCTC TTAGGAACAG AGGAGGCGCA 
GCGGCTGCAG TCGCCGGACT GGAGTGTGCC TCCCTCTTAG ACAAGCCCGT GCTGAGCCCG 
GAGTCCTCCA TCTACACCAC AGCAAGTGCC AGGACGCCCC CTCGCTCCCC AGAGAAACAC 
ACAGCAATAG CGTTCAACTT CGAGGCGGGG GTCCACCAGT ACATAGACAC CGACACTGAT 
GACGAGGGTC AGCTGCTCTA CAGCGTGGAC TCCAGTCCTC CCAAGAGTCT CCACGGGAGT 
ACCAGCCCCA AGTTCAGCCT TGGAGCTAGA ACAGAAAAGA ACCACTTCGA GAGCTCCCCG 
TTGCCCACCT CCCCTAAGTT CTTAAGGCCG AACTGTGTCT ACGCCTCAGA AGGGTTACCT 
GGGAAAGGTC CCGGGGCCCA AGAGAAATGC AAGCTGGAGA ACCACACCTC CCCCGATGTG 
CACATGCTGC CTGGGGGAGG AGCACACGGG AGCACTCGGG ATCAGAGTAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnb1 ( XM_017315732.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171221.1 Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X1, mRNA.

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CloneID	OMu13702
Clone ID Related Accession (Same CDS sequence)	XM_017315732.1 , NM_008420.4 , XM_017315733.2 , XM_017315733.1 , XM_017315732.2
Accession Version	XM_017315732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium voltage-gated channel subfamily B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCGG GCATGACGAA GCATGGCTCG CGCTCCACCA GCTCGCTGCC GCCCGAGCCC 
ATGGAGATCG TGCGCAGCAA GGCGTGCTCG CGCCGGGTGC GCCTCAACGT CGGGGGCCTG 
GCGCACGAGG TGCTGTGGCG CACTCTGGAC CGCCTGCCTC GCACGCGGCT GGGCAAGCTC 
CGGGACTGCA ACACGCACGA CTCTCTGCTC CAGGTGTGCG ACGACTACAG CCTCGAGGAC 
AACGAGTACT TCTTCGACCG CCACCCTGGC GCCTTCACCT CTATTCTCAA TTTCTACCGC 
ACCGGCCGGC TGCACATGAT GGAGGAGATG TGCGCGCTGA GCTTCAGCCA GGAGCTGGAC 
TACTGGGGCA TCGATGAGAT CTACCTGGAG TCCTGCTGCC AGGCCCGCTA CCACCAAAAG 
AAGGAGCAGA TGAACGAGGA GCTGAAGCGG GAGGCTGAGA CGCTGCGGGA GCGGGAGGGC 
GAGGAGTTCG ACAACACGTG CTGTGCTGAG AAGAGGAAGA AACTTTGGGA CCTGCTGGAG 
AAGCCCAACT CATCGGTGGC CGCCAAGATC CTGGCCATCA TCTCCATCAT GTTCATTGTC 
CTCTCCACCA TTGCCCTGTC ACTCAACACA CTGCCTGAGC TACAGAGCCT GGACGAATTC 
GGCCAGAGCA CGGACAACCC GCAGCTGGCA CACGTGGAGG CTGTGTGCAT CGCGTGGTTC 
ACCATGGAGT ACTTGCTGAG GTTCCTGTCC TCGCCCAAGA AATGGAAGTT CTTTAAGGGC 
CCCCTCAACG CCATTGACTT ACTGGCCATC CTGCCCTACT ACGTCACCAT CTTCCTCACA 
GAATCCAACA AGAGCGTGCT GCAGTTCCAG AATGTGCGCC GTGTGGTCCA GATCTTCCGC 
ATCATGCGCA TCCTGCGCAT CCTGAAGTTG GCCCGCCACT CCACCGGTCT GCAGTCCTTG 
GGCTTCACGC TGCGCAGGAG CTACAACGAG CTGGGCTTGC TCATCCTCTT CCTCGCCATG 
GGCATCATGA TCTTCTCCAG CCTGGTCTTC TTTGCCGAGA AGGATGAGGA TGACACCAAG 
TTCAAAAGCA TCCCCGCCTC TTTCTGGTGG GCTACCATCA CCATGACGAC CGTTGGTTAC 
GGAGACATCT ACCCTAAGAC TCTCCTGGGG AAAATCGTGG GGGGCCTCTG TTGCATTGCC 
GGTGTCCTGG TGATTGCCCT CCCCATTCCA ATTATCGTCA ATAACTTCTC CGAGTTCTAC 
AAGGAGCAGA AGCGCCAGGA GAAAGCCATC AAGCGGAGAG AGGCTCTGGA GAGAGCCAAG 
AGGAACGGCA GCATCGTGTC CATGAACATG AAGGATGCCT TCGCCCGGAG CATCGAGATG 
ATGGACATCG TGGTGGAGAA AAATGGAGAG GGCGTGGCTA AGAAGGACAA GGTGCAGGAT 
AACCACCTGT CTCCCAACAA GTGGAAATGG ACCAAGAGGG CGCTCTCCGA GACCAGCTCG 
AGCAAGTCCT TTGAAACCAA GGAGCAGGGA TCTCCTGAGA AGGCCAGGTC CTCGTCTAGT 
CCGCAGCACT TGAACGTCCA GCAGCTGCAA GACATGTACA GCAAGATGGC CAAGACCCAG 
TCTCAACCCA TCCTCAACAC CAAGGAGATG GCGCCGCAGA GCCAGCCGCA GGAAGAACTG 
GAGATGGGCA GCATGCCCAG CCCCGTGGCC CCTCTGCCCA CGCGCACAGA GGGCGTCATC 
GACATGCGCA GCATGTCTAG CATCGACAGC TTCATCAGCT GCGCCACGGA CTTCCCTGAA 
GCCACCAGAT TCTCCCACAG TCCTCTGGCA TCCCTCTCCG GCAAGTCTGG GGGCAGCACA 
GCCCCGGAGG TGGGCTGGCG GGGGGCTCTG GGTGCCAGCG GCGGGAGACT CATGGAGACC 
AACCCCATCC CCGAGGCCAG CCGCTCTGGT TTCTTCGTGG AGAGCCCCCG GAGTTCCATG 
AAGACCCACA ACCCCATGAA GCTGCGAGCG CTCAAGGTTA ACTTCCTGGA GGGCGATCCC 
ACCCCGCTGC TACCGGCTCT GGGCTTGTAT CACGATCCTC TTAGGAACAG AGGAGGCGCA 
GCGGCTGCAG TCGCCGGACT GGAGTGTGCC TCCCTCTTAG ACAAGCCCGT GCTGAGCCCG 
GAGTCCTCCA TCTACACCAC AGCAAGTGCC AGGACGCCCC CTCGCTCCCC AGAGAAACAC 
ACAGCAATAG CGTTCAACTT CGAGGCGGGG GTCCACCAGT ACATAGACAC CGACACTGAT 
GACGAGGGTC AGCTGCTCTA CAGCGTGGAC TCCAGTCCTC CCAAGAGTCT CCACGGGAGT 
ACCAGCCCCA AGTTCAGCCT TGGAGCTAGA ACAGAAAAGA ACCACTTCGA GAGCTCCCCG 
TTGCCCACCT CCCCTAAGTT CTTAAGGCCG AACTGTGTCT ACGCCTCAGA AGGGTTACCT 
GGGAAAGGTC CCGGGGCCCA AGAGAAATGC AAGCTGGAGA ACCACACCTC CCCCGATGTG 
CACATGCTGC CTGGGGGAGG AGCACACGGG AGCACTCGGG ATCAGAGTAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171221.1
CDS	418..2991
Translation	MPAGMTKHGSRSTSSLPPEPMEIVRSKACSRRVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDCNTHDSLLQVCDDYSLEDNEYFFDRHPGAFTSILNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREAETLREREGEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPELQSLDEFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNMKDAFARSIEMMDIVVEKNGEGVAKKDKVQDNHLSPNKWKWTKRALSETSSSKSFETKEQGSPEKARSSSSPQHLNVQQLQDMYSKMAKTQSQPILNTKEMAPQSQPQEELEMGSMPSPVAPLPTRTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLASLSGKSGGSTAPEVGWRGALGASGGRLMETNPIPEASRSGFFVESPRSSMKTHNPMKLRALKVNFLEGDPTPLLPALGLYHDPLRNRGGAAAAVAGLECASLLDKPVLSPESSIYTTASARTPPRSPEKHTAIAFNFEAGVHQYIDTDTDDEGQLLYSVDSSPPKSLHGSTSPKFSLGARTEKNHFESSPLPTSPKFLRPNCVYASEGLPGKGPGAQEKCKLENHTSPDVHMLPGGGAHGSTRDQSI

Target ORF information:

RefSeq Version	XM_017315732.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium voltage gated channel, Shab-related subfamily, member 1 (Kcnb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315732.2

ORF Insert Sequence:

ATGCCGGCGG GCATGACGAA GCATGGCTCG CGCTCCACCA GCTCGCTGCC GCCCGAGCCC 
ATGGAGATCG TGCGCAGCAA GGCGTGCTCG CGCCGGGTGC GCCTCAACGT CGGGGGCCTG 
GCGCACGAGG TGCTGTGGCG CACTCTGGAC CGCCTGCCTC GCACGCGGCT GGGCAAGCTC 
CGGGACTGCA ACACGCACGA CTCTCTGCTC CAGGTGTGCG ACGACTACAG CCTCGAGGAC 
AACGAGTACT TCTTCGACCG CCACCCTGGC GCCTTCACCT CTATTCTCAA TTTCTACCGC 
ACCGGCCGGC TGCACATGAT GGAGGAGATG TGCGCGCTGA GCTTCAGCCA GGAGCTGGAC 
TACTGGGGCA TCGATGAGAT CTACCTGGAG TCCTGCTGCC AGGCCCGCTA CCACCAAAAG 
AAGGAGCAGA TGAACGAGGA GCTGAAGCGG GAGGCTGAGA CGCTGCGGGA GCGGGAGGGC 
GAGGAGTTCG ACAACACGTG CTGTGCTGAG AAGAGGAAGA AACTTTGGGA CCTGCTGGAG 
AAGCCCAACT CATCGGTGGC CGCCAAGATC CTGGCCATCA TCTCCATCAT GTTCATTGTC 
CTCTCCACCA TTGCCCTGTC ACTCAACACA CTGCCTGAGC TACAGAGCCT GGACGAATTC 
GGCCAGAGCA CGGACAACCC GCAGCTGGCA CACGTGGAGG CTGTGTGCAT CGCGTGGTTC 
ACCATGGAGT ACTTGCTGAG GTTCCTGTCC TCGCCCAAGA AATGGAAGTT CTTTAAGGGC 
CCCCTCAACG CCATTGACTT ACTGGCCATC CTGCCCTACT ACGTCACCAT CTTCCTCACA 
GAATCCAACA AGAGCGTGCT GCAGTTCCAG AATGTGCGCC GTGTGGTCCA GATCTTCCGC 
ATCATGCGCA TCCTGCGCAT CCTGAAGTTG GCCCGCCACT CCACCGGTCT GCAGTCCTTG 
GGCTTCACGC TGCGCAGGAG CTACAACGAG CTGGGCTTGC TCATCCTCTT CCTCGCCATG 
GGCATCATGA TCTTCTCCAG CCTGGTCTTC TTTGCCGAGA AGGATGAGGA TGACACCAAG 
TTCAAAAGCA TCCCCGCCTC TTTCTGGTGG GCTACCATCA CCATGACGAC CGTTGGTTAC 
GGAGACATCT ACCCTAAGAC TCTCCTGGGG AAAATCGTGG GGGGCCTCTG TTGCATTGCC 
GGTGTCCTGG TGATTGCCCT CCCCATTCCA ATTATCGTCA ATAACTTCTC CGAGTTCTAC 
AAGGAGCAGA AGCGCCAGGA GAAAGCCATC AAGCGGAGAG AGGCTCTGGA GAGAGCCAAG 
AGGAACGGCA GCATCGTGTC CATGAACATG AAGGATGCCT TCGCCCGGAG CATCGAGATG 
ATGGACATCG TGGTGGAGAA AAATGGAGAG GGCGTGGCTA AGAAGGACAA GGTGCAGGAT 
AACCACCTGT CTCCCAACAA GTGGAAATGG ACCAAGAGGG CGCTCTCCGA GACCAGCTCG 
AGCAAGTCCT TTGAAACCAA GGAGCAGGGA TCTCCTGAGA AGGCCAGGTC CTCGTCTAGT 
CCGCAGCACT TGAACGTCCA GCAGCTGCAA GACATGTACA GCAAGATGGC CAAGACCCAG 
TCTCAACCCA TCCTCAACAC CAAGGAGATG GCGCCGCAGA GCCAGCCGCA GGAAGAACTG 
GAGATGGGCA GCATGCCCAG CCCCGTGGCC CCTCTGCCCA CGCGCACAGA GGGCGTCATC 
GACATGCGCA GCATGTCTAG CATCGACAGC TTCATCAGCT GCGCCACGGA CTTCCCTGAA 
GCCACCAGAT TCTCCCACAG TCCTCTGGCA TCCCTCTCCG GCAAGTCTGG GGGCAGCACA 
GCCCCGGAGG TGGGCTGGCG GGGGGCTCTG GGTGCCAGCG GCGGGAGACT CATGGAGACC 
AACCCCATCC CCGAGGCCAG CCGCTCTGGT TTCTTCGTGG AGAGCCCCCG GAGTTCCATG 
AAGACCCACA ACCCCATGAA GCTGCGAGCG CTCAAGGTTA ACTTCCTGGA GGGCGATCCC 
ACCCCGCTGC TACCGGCTCT GGGCTTGTAT CACGATCCTC TTAGGAACAG AGGAGGCGCA 
GCGGCTGCAG TCGCCGGACT GGAGTGTGCC TCCCTCTTAG ACAAGCCCGT GCTGAGCCCG 
GAGTCCTCCA TCTACACCAC AGCAAGTGCC AGGACGCCCC CTCGCTCCCC AGAGAAACAC 
ACAGCAATAG CGTTCAACTT CGAGGCGGGG GTCCACCAGT ACATAGACAC CGACACTGAT 
GACGAGGGTC AGCTGCTCTA CAGCGTGGAC TCCAGTCCTC CCAAGAGTCT CCACGGGAGT 
ACCAGCCCCA AGTTCAGCCT TGGAGCTAGA ACAGAAAAGA ACCACTTCGA GAGCTCCCCG 
TTGCCCACCT CCCCTAAGTT CTTAAGGCCG AACTGTGTCT ACGCCTCAGA AGGGTTACCT 
GGGAAAGGTC CCGGGGCCCA AGAGAAATGC AAGCTGGAGA ACCACACCTC CCCCGATGTG 
CACATGCTGC CTGGGGGAGG AGCACACGGG AGCACTCGGG ATCAGAGTAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reduced sensory synaptic excitation impairs motor neuron function via Kv2.1 in spinal muscular atrophy.
Nature neuroscience20(7)905-916(2017 Jul)
Fletcher EV,Simon CM,Pagiazitis JG,Chalif JI,Vukojicic A,Drobac E,Wang X,Mentis GZ

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Kcnb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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