Itm2b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itm2b
Entrez Gene ID	16432
Full Name	integral membrane protein 2B
Synonyms	AI256040,Bri,Bri2,Bricd2b,D14Sel6,E25BMM,imBRI2
General protein information	Preferred Names integral membrane protein 2B Names integral membrane protein 2B Integral membrane protein 2B (E25B protein) immature BRI2 transmembrane protein BRI
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 D3\|14 38.88 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008410.3	NP_032436.1	integral membrane protein 2B precursor
NM_008410.2	NP_032436.1	integral membrane protein 2B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Itm2b	NP_032436.1
Danio rerio (zebrafish)	itm2ba	NP_955940.1
	ITM2B	XP_002800791.1
Bos taurus (cattle)	ITM2B	NP_001030265.1
Rattus norvegicus (Norway rat)	Itm2b	NP_001006964.1
Canis lupus familiaris (dog)	ITM2B	XP_005633935.1
Gallus gallus (chicken)	ITM2B	NP_990416.1
Homo sapiens (human)	ITM2B	NP_068839.1
Pan troglodytes (chimpanzee)	ITM2B	NP_001092017.1
Xenopus tropicalis (tropical clawed frog)	itm2b	NP_989241.1

Gene Ontology
Bibliography

Related articles in PubMed

Haploinsufficiency of the Insulin Receptor in the Presence of a Splice-Site Mutation in Ppp2r2a Results in a Novel Digenic Mouse Model of Type 2 Diabetes.
Goldsworthy M, Bai Y, Li CM, Ge H, Lamas E, Hilton H, Esapa CT, Baker D, Baron W, Juan T, V?niant MM, Lloyd DJ, Cox RD
Diabetes65(5)1434-46(2016 05)

Reduced Fertility and Altered Epididymal and Sperm Integrity in Mice Lacking ADAM7.
Choi H, Han C, Jin S, Kwon JT, Kim J, Jeong J, Kim J, Ham S, Jeon S, Yoo YJ, Cho C
Biology of reproduction93(3)70(2015 Sep)

Loss of LKB1 leads to impaired epithelial integrity and cell extrusion in the early mouse embryo.
Krawchuk D, Anani S, Honma-Yamanaka N, Polito S, Shafik M, Yamanaka Y
Journal of cell science128(5)1011-22(2015 Mar)

The familial dementia gene revisited: a missense mutation revealed by whole-exome sequencing identifies ITM2B as a candidate gene underlying a novel autosomal dominant retinal dystrophy in a large family.
Audo I, Bujakowska K, Orhan E, El Shamieh S, Sennlaub F, Guillonneau X, Antonio A, Michiels C, Lancelot ME, Letexier M, Saraiva JP, Nguyen H, Luu TD, L?veillard T, Poch O, Dollfus H, Paques M, Goureau O, Mohand-Sa?d S, Bhattacharya SS, Sahel JA, Zeitz C
Human molecular genetics23(2)491-501(2014 Jan)

An intracellular threonine of amyloid-? precursor protein mediates synaptic plasticity deficits and memory loss.
Lombino F, Biundo F, Tamayev R, Arancio O, D'Adamio L
PloS one8(2)e57120(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that double transgenic (Tg-FDD-Tau) mice showed a significant decrease in synaptophysin levels.
Title: Increased tau phosphorylation and tau truncation, and decreased synaptophysin levels in mutant BRI2/tau transgenic mice.

BRI2 protein regulates beta-amyloid degradation by increasing levels of secreted insulin-degrading enzyme (IDE).
Title: BRI2 protein regulates ?-amyloid degradation by increasing levels of secreted insulin-degrading enzyme (IDE).

Suggest that Adam7 functions in fertilization through the formation of a complex with heat shock protein 5, calnexin and Itm2b during capacitation in sperm.
Title: Identification of heat shock protein 5, calnexin and integral membrane protein 2B as Adam7-interacting membrane proteins in mouse sperm.

Itm2b mRNA was differentially expressed in mouse male reproductive tissues, during sexual maturation and up-regulated by testosterone.
Title: Expression and regulation of type II integral membrane protein family members in mouse male reproductive tissues.

BH3-only protein ITM2Bs is able to induce apoptotic cell death in p53+/+, as well as in p53-/- cell lines.
Title: Induction of p53-independent apoptosis by the BH3-only protein ITM2Bs.

The following Itm2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itm2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01880	NM_008410.3 Latest version!	Mus musculus integral membrane protein 2B (Itm2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	Quote Price
View Old Accession Versions >>
OMu01880	NM_008410.2	Mus musculus integral membrane protein 2B (Itm2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	Quote Price
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itm2b ( NM_008410.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032436.1 Mus musculus integral membrane protein 2B (Itm2b), mRNA.

Price & Availability↑

CloneID	OMu01880
Clone ID Related Accession (Same CDS sequence)	NM_008410.3 , NM_008410.2
Accession Version	NM_008410.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	integral membrane protein 2B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154718.2. On Apr 15, 2018 this sequence version replaced NM_008410.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK151714.1, AK150378.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGG TGACGTTCAA CTCGGCGCTG GCCCAGAAGG AGGCCAAGAA GGACGAGCCC 
AAGAGCAGCG AGGAGGCGCT CATCGTCCCT CCGGATGCCG TGGCGGTGGA TTGCAAGGAC 
CCGGGTGACG TGGTTCCGGT TGGACAGAGG AGAGCGTGGT GTTGGTGCAT GTGTTTCGGA 
CTGGCCTTCA TGCTTGCTGG CGTCATCCTC GGAGGGGCGT ACCTGTACAA GTATTTTGCT 
CTTCAGCCAG ATGATGTGTA CTACTGTGGA CTAAAGTACA TCAAAGATGA CGTCATCCTG 
AACGAGCCTT CTGCGGATGC CCCAGCTGCT CGCTACCAGA CAATTGAAGA GAACATTAAG 
ATCTTTGAGG AAGACGCAGT GGAATTCATC AGTGTGCCTG TACCAGAGTT TGCGGACAGC 
GATCCTGCCA ACATTGTGCA CGACTTCAAC AAGAAACTCA CTGCTTATTT GGACCTTAAC 
CTGGACAAGT GCTACGTGAT TCCTCTGAAC ACTTCCATCG TTATGCCGCC CAAAAACCTG 
CTGGAGCTCC TTATTAACAT TAAGGCCGGG ACCTACCTGC CTCAGTCCTA CCTTATCCAT 
GAGCACATGG TGATCACCGA CCGCATCGAG AACGTGGACA ACCTGGGCTT CTTCATCTAC 
CGACTGTGTC ACGACAAGGA GACCTACAAA CTGCAGCGCC GGGAAACAAT TAGAGGTATT 
CAGAAGCGGG AAGCCAGTAA CTGTTTCACC ATTCGGCATT TTGAGAACAA ATTTGCTGTG 
GAGACTTTAA TTTGTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032436.1
CDS	164..964
Translation	MVKVTFNSALAQKEAKKDEPKSSEEALIVPPDAVAVDCKDPGDVVPVGQRRAWCWCMCFGLAFMLAGVILGGAYLYKYFALQPDDVYYCGLKYIKDDVILNEPSADAPAARYQTIEENIKIFEEDAVEFISVPVPEFADSDPANIVHDFNKKLTAYLDLNLDKCYVIPLNTSIVMPPKNLLELLINIKAGTYLPQSYLIHEHMVITDRIENVDNLGFFIYRLCHDKETYKLQRRETIRGIQKREASNCFTIRHFENKFAVETLICS

Target ORF information:

RefSeq Version	NM_008410.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus integral membrane protein 2B (Itm2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008410.3

ORF Insert Sequence:

ATGGTGAAGG TGACGTTCAA CTCGGCGCTG GCCCAGAAGG AGGCCAAGAA GGACGAGCCC 
AAGAGCAGCG AGGAGGCGCT CATCGTCCCT CCGGATGCCG TGGCGGTGGA TTGCAAGGAC 
CCGGGTGACG TGGTTCCGGT TGGACAGAGG AGAGCGTGGT GTTGGTGCAT GTGTTTCGGA 
CTGGCCTTCA TGCTTGCTGG CGTCATCCTC GGAGGGGCGT ACCTGTACAA GTATTTTGCT 
CTTCAGCCAG ATGATGTGTA CTACTGTGGA CTAAAGTACA TCAAAGATGA CGTCATCCTG 
AACGAGCCTT CTGCGGATGC CCCAGCTGCT CGCTACCAGA CAATTGAAGA GAACATTAAG 
ATCTTTGAGG AAGACGCAGT GGAATTCATC AGTGTGCCTG TACCAGAGTT TGCGGACAGC 
GATCCTGCCA ACATTGTGCA CGACTTCAAC AAGAAACTCA CTGCTTATTT GGACCTTAAC 
CTGGACAAGT GCTACGTGAT TCCTCTGAAC ACTTCCATCG TTATGCCGCC CAAAAACCTG 
CTGGAGCTCC TTATTAACAT TAAGGCCGGG ACCTACCTGC CTCAGTCCTA CCTTATCCAT 
GAGCACATGG TGATCACCGA CCGCATCGAG AACGTGGACA ACCTGGGCTT CTTCATCTAC 
CGACTGTGTC ACGACAAGGA GACCTACAAA CTGCAGCGCC GGGAAACAAT TAGAGGTATT 
CAGAAGCGGG AAGCCAGTAA CTGTTTCACC ATTCGGCATT TTGAGAACAA ATTTGCTGTG 
GAGACTTTAA TTTGTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itm2b ( NM_008410.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032436.1 Mus musculus integral membrane protein 2B (Itm2b), mRNA.

Price & Availability↑

CloneID	OMu01880
Clone ID Related Accession (Same CDS sequence)	NM_008410.3 , NM_008410.2
Accession Version	NM_008410.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Mus musculus(house mouse)
Product	integral membrane protein 2B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC154718.2. On or before Mar 15, 2011 this sequence version replaced XR_106869.1, XR_105626.1, NM_008410.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U76253.1, AK151714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAAGG TGACGTTCAA CTCGGCGCTG GCCCAGAAGG AGGCCAAGAA GGACGAGCCC 
AAGAGCAGCG AGGAGGCGCT CATCGTCCCT CCGGATGCCG TGGCGGTGGA TTGCAAGGAC 
CCGGGTGACG TGGTTCCGGT TGGACAGAGG AGAGCGTGGT GTTGGTGCAT GTGTTTCGGA 
CTGGCCTTCA TGCTTGCTGG CGTCATCCTC GGAGGGGCGT ACCTGTACAA GTATTTTGCT 
CTTCAGCCAG ATGATGTGTA CTACTGTGGA CTAAAGTACA TCAAAGATGA CGTCATCCTG 
AACGAGCCTT CTGCGGATGC CCCAGCTGCT CGCTACCAGA CAATTGAAGA GAACATTAAG 
ATCTTTGAGG AAGACGCAGT GGAATTCATC AGTGTGCCTG TACCAGAGTT TGCGGACAGC 
GATCCTGCCA ACATTGTGCA CGACTTCAAC AAGAAACTCA CTGCTTATTT GGACCTTAAC 
CTGGACAAGT GCTACGTGAT TCCTCTGAAC ACTTCCATCG TTATGCCGCC CAAAAACCTG 
CTGGAGCTCC TTATTAACAT TAAGGCCGGG ACCTACCTGC CTCAGTCCTA CCTTATCCAT 
GAGCACATGG TGATCACCGA CCGCATCGAG AACGTGGACA ACCTGGGCTT CTTCATCTAC 
CGACTGTGTC ACGACAAGGA GACCTACAAA CTGCAGCGCC GGGAAACAAT TAGAGGTATT 
CAGAAGCGGG AAGCCAGTAA CTGTTTCACC ATTCGGCATT TTGAGAACAA ATTTGCTGTG 
GAGACTTTAA TTTGTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032436.1
CDS	146..946
Translation	MVKVTFNSALAQKEAKKDEPKSSEEALIVPPDAVAVDCKDPGDVVPVGQRRAWCWCMCFGLAFMLAGVILGGAYLYKYFALQPDDVYYCGLKYIKDDVILNEPSADAPAARYQTIEENIKIFEEDAVEFISVPVPEFADSDPANIVHDFNKKLTAYLDLNLDKCYVIPLNTSIVMPPKNLLELLINIKAGTYLPQSYLIHEHMVITDRIENVDNLGFFIYRLCHDKETYKLQRRETIRGIQKREASNCFTIRHFENKFAVETLICS

Target ORF information:

RefSeq Version	NM_008410.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus integral membrane protein 2B (Itm2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008410.2

ORF Insert Sequence:

ATGGTGAAGG TGACGTTCAA CTCGGCGCTG GCCCAGAAGG AGGCCAAGAA GGACGAGCCC 
AAGAGCAGCG AGGAGGCGCT CATCGTCCCT CCGGATGCCG TGGCGGTGGA TTGCAAGGAC 
CCGGGTGACG TGGTTCCGGT TGGACAGAGG AGAGCGTGGT GTTGGTGCAT GTGTTTCGGA 
CTGGCCTTCA TGCTTGCTGG CGTCATCCTC GGAGGGGCGT ACCTGTACAA GTATTTTGCT 
CTTCAGCCAG ATGATGTGTA CTACTGTGGA CTAAAGTACA TCAAAGATGA CGTCATCCTG 
AACGAGCCTT CTGCGGATGC CCCAGCTGCT CGCTACCAGA CAATTGAAGA GAACATTAAG 
ATCTTTGAGG AAGACGCAGT GGAATTCATC AGTGTGCCTG TACCAGAGTT TGCGGACAGC 
GATCCTGCCA ACATTGTGCA CGACTTCAAC AAGAAACTCA CTGCTTATTT GGACCTTAAC 
CTGGACAAGT GCTACGTGAT TCCTCTGAAC ACTTCCATCG TTATGCCGCC CAAAAACCTG 
CTGGAGCTCC TTATTAACAT TAAGGCCGGG ACCTACCTGC CTCAGTCCTA CCTTATCCAT 
GAGCACATGG TGATCACCGA CCGCATCGAG AACGTGGACA ACCTGGGCTT CTTCATCTAC 
CGACTGTGTC ACGACAAGGA GACCTACAAA CTGCAGCGCC GGGAAACAAT TAGAGGTATT 
CAGAAGCGGG AAGCCAGTAA CTGTTTCACC ATTCGGCATT TTGAGAACAA ATTTGCTGTG 
GAGACTTTAA TTTGTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Haploinsufficiency of the Insulin Receptor in the Presence of a Splice-Site Mutation in Ppp2r2a Results in a Novel Digenic Mouse Model of Type 2 Diabetes.
Diabetes65(5)1434-46(2016 05)
Goldsworthy M,Bai Y,Li CM,Ge H,Lamas E,Hilton H,Esapa CT,Baker D,Baron W,Juan T,V?niant MM,Lloyd DJ,Cox RD

Reduced Fertility and Altered Epididymal and Sperm Integrity in Mice Lacking ADAM7.
Biology of reproduction93(3)70(2015 Sep)
Choi H,Han C,Jin S,Kwon JT,Kim J,Jeong J,Kim J,Ham S,Jeon S,Yoo YJ,Cho C

Loss of LKB1 leads to impaired epithelial integrity and cell extrusion in the early mouse embryo.
Journal of cell science128(5)1011-22(2015 Mar)
Krawchuk D,Anani S,Honma-Yamanaka N,Polito S,Shafik M,Yamanaka Y

The familial dementia gene revisited: a missense mutation revealed by whole-exome sequencing identifies ITM2B as a candidate gene underlying a novel autosomal dominant retinal dystrophy in a large family.
Human molecular genetics23(2)491-501(2014 Jan)
Audo I,Bujakowska K,Orhan E,El Shamieh S,Sennlaub F,Guillonneau X,Antonio A,Michiels C,Lancelot ME,Letexier M,Saraiva JP,Nguyen H,Luu TD,L?veillard T,Poch O,Dollfus H,Paques M,Goureau O,Mohand-Sa?d S,Bhattacharya SS,Sahel JA,Zeitz C

An intracellular threonine of amyloid-? precursor protein mediates synaptic plasticity deficits and memory loss.
PloS one8(2)e57120(2013)
Lombino F,Biundo F,Tamayev R,Arancio O,D'Adamio L