Itga5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itga5
Entrez Gene ID	16402
Full Name	integrin alpha 5 (fibronectin receptor alpha)
Synonyms	Cd49e,Fnra,VLA5
General protein information	Preferred Names integrin alpha 5 (fibronectin receptor alpha) Names integrin alpha-5 CD49 antigen-like family member E fibronectin receptor alpha polypeptide fibronectin receptor subunit alpha integrin alpha-F
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 F3\|15 58.9 cM
Summary	The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain, which is proteolytically processed to generate light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signaling. Integrin alpha 5 and integrin alpha V chains are produced by distinct genes. Homozygous knockout mice for this gene exhibit embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001314041.1	NP_001300970.1	integrin alpha-5 isoform 2
NM_010577.4	NP_034707.5	integrin alpha-5 isoform 1 preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Itga5	NP_001101588.1
Pan troglodytes (chimpanzee)	ITGA5	XP_003313671.2
Homo sapiens (human)	ITGA5	NP_002196.2
	ITGA5	XP_001110116.2
Mus musculus (house mouse)	Itga5	NP_034707.3
Canis lupus familiaris (dog)	ITGA5	XP_005636796.1
Bos taurus (cattle)	ITGA5	NP_001159972.1
Xenopus tropicalis (tropical clawed frog)	itga5	XP_002935705.2
Danio rerio (zebrafish)	itga5	NP_001004288.2

Gene Ontology

The following Itga5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itga5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu83573	NM_001314041.1 Latest version!	Mus musculus integrin alpha 5 (fibronectin receptor alpha) (Itga5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu22491	NM_010577.4 Latest version!	Mus musculus integrin alpha 5 (fibronectin receptor alpha) (Itga5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itga5 ( NM_001314041.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300970.1 Mus musculus integrin alpha 5 (fibronectin receptor alpha) (Itga5), transcript variant 2, mRNA.

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CloneID	OMu83573
Clone ID Related Accession (Same CDS sequence)	NM_001314041.1
Accession Version	NM_001314041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Mus musculus(house mouse)
Product	integrin alpha-5 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC131721.4, BC050943.1 and AK142241.1. Transcript Variant: This variant (2) differs in the 5' UTR, uses a downstream translation start site, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053431.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCCT ACTTCGGCTA TGCTGTGGCT GCCACTGATA CCAATGGAGA TGGGCTAGAT 
GACCTACTGG TAGGGGCACC CCTGCTCATG GAGCGGACAG CTGATGGGAG ACCTCAGGAG 
GTGGGCAGGG TCTACATCTA TCTGCAGCGC CCAGCGGGCA TAGATCCCAC ACCCACCCTC 
ACCCTCACTG GGCAAGATGA GTTCAGCCGA TTCGGCAGCT CCTTGACACC CTTGGGGGAC 
CTGGACCAAG ACGGCTACAA TGATGTCGCC ATTGGGGCTC CATTTGGTGG GGAGGCCCAG 
CAGGGAGTCG TATTTATATT CCCGGGAGGC CCAGGAGGAC TGAGCACTAA ACCTTCCCAG 
GTTTTGCAGC CTCTTTGGGC AGCTGGCCGT ACCCCAGACT TCTTTGGCTC TGCCCTTCGA 
GGAGGACGAG ATCTGGATGG CAATGGATAC CCTGATCTAA TCGTTGGATC CTTTGGTGTG 
GACAAGGCTC TGGTGTACAG AGGGCGGCCC ATCATATCTG CCAGCGCATC TCTCACCATC 
TTCCCCTCCA TGTTCAACCC AGAGGAGCGC AGTTGCAGCC TGGAAGGGAA CCCTGTGTCC 
TGCATCAACC TTAGCTTCTG CCTCAATGCC TCTGGAAAAC ATGTCCCCAA CTCTATAGGC 
TTCGAGGTGG AACTCCAACT GGACTGGCAG AAGCAAAAGG GAGGGGTCCG GCGGGCACTG 
TTCCTGACTT CCAAGCAGGC CACCCTTACC CAGACCCTGC TTATCCAGAA TGGGGCTCGG 
GAGGACTGCA GGGAGATGAA GATCTACCTC AGGAATGAAT CAGAATTCAG AGACAAACTC 
TCCCCAATTC ACATTGCCCT CAACTTCTCC TTGGACCCCA AAGCTCCCAT GGACAGCCAT 
GGCCTCCGGC CAGTTCTACA CTACCAAAGC AAAAGCAGGA TAGAGGACAA GGCCCAGATC 
TTGCTGGACT GTGGTGAAGA CAATATCTGT GTGCCTGACC TGCAGCTGGA TGTGTATGGG 
GAGAAGAAAC ATGTGTACCT GGGTGACAAG AACGCACTGA ACCTGACATT CCATGCCCAA 
AATCTGGGTG AGGGCGGTGC CTATGAAGCC GAGCTTCGGG TCACAGCCCC TCTAGAGGCC 
GAGTACTCAG GACTTGTCAG ACACCCAGGG AACTTCTCCA GCCTGAGCTG TGACTACTTT 
GCTGTGAACC AGAGCCGCCA GCTGGTGTGT GACCTGGGCA ACCCCATGAA GCTCTGGGGT 
GGCCTTCGGT TCACTGTTCC TCATCTTCAA GACACAAAGA AAACCATCCA GTTTGACTTT 
CAGATCCTCA GCAAGAACCT GAACAACTCA CAAAGCAACG TGGTCTCCTT CCCACTCTCG 
GTGGAGGCTC AAGCCCAGGT CTCCCTTAAT GGTGTCTCCA AGCCTGAAGC TGTGATTTTC 
CCAGTCAGCG ACTGGAATCC TCAAGACCAG CCTCAGAAGG AGGAAGACTT GGGCCCAGCT 
GTCCACCATG TCTACGAGCT CATCAACCAG GGGCCCAGCT CCATCAGCCA GGGTGTGCTG 
GAGCTCAGCT GTCCACAGGC TCTGGAAGGC CAACAGCTCC TCTATGTGAC CAAGGTGACA 
GGACTCAGCA ACTGCACCTC CAACTACACC CCCAACTCAC AGGGCCTGGA GTTGGATCCA 
GAGACCTCTC CACACCACCT GCAGAAACGA GAGGCTCCAG GGAGGAGTTC TACTGCCTCA 
GGAACACAAG TTCTGAAATG CCCTGAAGCC AAGTGTTTCA GGCTGCGCTG TGAGTTTGGG 
CCACTGCACC GCCAAGAGAG CCGTAGTCTG CAGCTGCATT TCCGAGTCTG GGCCAAGACC 
TTCTTGCAGC GGGAATACCA GCCATTTAGC CTTCAGTGTG AGGCTGTATA TGAAGCTCTG 
AAGATGCCCT ACCAGATCCT GCCTCGGCAG CTTCCCCAAA AGAAACTTCA GGTGGCCACA 
GCCGTGCAGT GGACCAAGGC AGAAGGCAGC AATGGTGTCC CGTTGTGGAT CATCATCCTA 
GCCATTCTTT TTGGCCTCCT GCTCCTAGGT CTGCTCATCT ACGTCCTCTA CAAGCTCGGC 
TTCTTCAAAC GTTCCCTCCC CTACGGCACA GCCATGGAAA AAGCTCAGCT CAAGCCTCCA 
GCCACCTCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300970.1
CDS	1298..3475
Translation	MASYFGYAVAATDTNGDGLDDLLVGAPLLMERTADGRPQEVGRVYIYLQRPAGIDPTPTLTLTGQDEFSRFGSSLTPLGDLDQDGYNDVAIGAPFGGEAQQGVVFIFPGGPGGLSTKPSQVLQPLWAAGRTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKALVYRGRPIISASASLTIFPSMFNPEERSCSLEGNPVSCINLSFCLNASGKHVPNSIGFEVELQLDWQKQKGGVRRALFLTSKQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPKAPMDSHGLRPVLHYQSKSRIEDKAQILLDCGEDNICVPDLQLDVYGEKKHVYLGDKNALNLTFHAQNLGEGGAYEAELRVTAPLEAEYSGLVRHPGNFSSLSCDYFAVNQSRQLVCDLGNPMKLWGGLRFTVPHLQDTKKTIQFDFQILSKNLNNSQSNVVSFPLSVEAQAQVSLNGVSKPEAVIFPVSDWNPQDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTKVTGLSNCTSNYTPNSQGLELDPETSPHHLQKREAPGRSSTASGTQVLKCPEAKCFRLRCEFGPLHRQESRSLQLHFRVWAKTFLQREYQPFSLQCEAVYEALKMPYQILPRQLPQKKLQVATAVQWTKAEGSNGVPLWIIILAILFGLLLLGLLIYVLYKLGFFKRSLPYGTAMEKAQLKPPATSDA

Target ORF information:

RefSeq Version	NM_001314041.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin alpha 5 (fibronectin receptor alpha) (Itga5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001314041.1

ORF Insert Sequence:

ATGGCCTCCT ACTTCGGCTA TGCTGTGGCT GCCACTGATA CCAATGGAGA TGGGCTAGAT 
GACCTACTGG TAGGGGCACC CCTGCTCATG GAGCGGACAG CTGATGGGAG ACCTCAGGAG 
GTGGGCAGGG TCTACATCTA TCTGCAGCGC CCAGCGGGCA TAGATCCCAC ACCCACCCTC 
ACCCTCACTG GGCAAGATGA GTTCAGCCGA TTCGGCAGCT CCTTGACACC CTTGGGGGAC 
CTGGACCAAG ACGGCTACAA TGATGTCGCC ATTGGGGCTC CATTTGGTGG GGAGGCCCAG 
CAGGGAGTCG TATTTATATT CCCGGGAGGC CCAGGAGGAC TGAGCACTAA ACCTTCCCAG 
GTTTTGCAGC CTCTTTGGGC AGCTGGCCGT ACCCCAGACT TCTTTGGCTC TGCCCTTCGA 
GGAGGACGAG ATCTGGATGG CAATGGATAC CCTGATCTAA TCGTTGGATC CTTTGGTGTG 
GACAAGGCTC TGGTGTACAG AGGGCGGCCC ATCATATCTG CCAGCGCATC TCTCACCATC 
TTCCCCTCCA TGTTCAACCC AGAGGAGCGC AGTTGCAGCC TGGAAGGGAA CCCTGTGTCC 
TGCATCAACC TTAGCTTCTG CCTCAATGCC TCTGGAAAAC ATGTCCCCAA CTCTATAGGC 
TTCGAGGTGG AACTCCAACT GGACTGGCAG AAGCAAAAGG GAGGGGTCCG GCGGGCACTG 
TTCCTGACTT CCAAGCAGGC CACCCTTACC CAGACCCTGC TTATCCAGAA TGGGGCTCGG 
GAGGACTGCA GGGAGATGAA GATCTACCTC AGGAATGAAT CAGAATTCAG AGACAAACTC 
TCCCCAATTC ACATTGCCCT CAACTTCTCC TTGGACCCCA AAGCTCCCAT GGACAGCCAT 
GGCCTCCGGC CAGTTCTACA CTACCAAAGC AAAAGCAGGA TAGAGGACAA GGCCCAGATC 
TTGCTGGACT GTGGTGAAGA CAATATCTGT GTGCCTGACC TGCAGCTGGA TGTGTATGGG 
GAGAAGAAAC ATGTGTACCT GGGTGACAAG AACGCACTGA ACCTGACATT CCATGCCCAA 
AATCTGGGTG AGGGCGGTGC CTATGAAGCC GAGCTTCGGG TCACAGCCCC TCTAGAGGCC 
GAGTACTCAG GACTTGTCAG ACACCCAGGG AACTTCTCCA GCCTGAGCTG TGACTACTTT 
GCTGTGAACC AGAGCCGCCA GCTGGTGTGT GACCTGGGCA ACCCCATGAA GCTCTGGGGT 
GGCCTTCGGT TCACTGTTCC TCATCTTCAA GACACAAAGA AAACCATCCA GTTTGACTTT 
CAGATCCTCA GCAAGAACCT GAACAACTCA CAAAGCAACG TGGTCTCCTT CCCACTCTCG 
GTGGAGGCTC AAGCCCAGGT CTCCCTTAAT GGTGTCTCCA AGCCTGAAGC TGTGATTTTC 
CCAGTCAGCG ACTGGAATCC TCAAGACCAG CCTCAGAAGG AGGAAGACTT GGGCCCAGCT 
GTCCACCATG TCTACGAGCT CATCAACCAG GGGCCCAGCT CCATCAGCCA GGGTGTGCTG 
GAGCTCAGCT GTCCACAGGC TCTGGAAGGC CAACAGCTCC TCTATGTGAC CAAGGTGACA 
GGACTCAGCA ACTGCACCTC CAACTACACC CCCAACTCAC AGGGCCTGGA GTTGGATCCA 
GAGACCTCTC CACACCACCT GCAGAAACGA GAGGCTCCAG GGAGGAGTTC TACTGCCTCA 
GGAACACAAG TTCTGAAATG CCCTGAAGCC AAGTGTTTCA GGCTGCGCTG TGAGTTTGGG 
CCACTGCACC GCCAAGAGAG CCGTAGTCTG CAGCTGCATT TCCGAGTCTG GGCCAAGACC 
TTCTTGCAGC GGGAATACCA GCCATTTAGC CTTCAGTGTG AGGCTGTATA TGAAGCTCTG 
AAGATGCCCT ACCAGATCCT GCCTCGGCAG CTTCCCCAAA AGAAACTTCA GGTGGCCACA 
GCCGTGCAGT GGACCAAGGC AGAAGGCAGC AATGGTGTCC CGTTGTGGAT CATCATCCTA 
GCCATTCTTT TTGGCCTCCT GCTCCTAGGT CTGCTCATCT ACGTCCTCTA CAAGCTCGGC 
TTCTTCAAAC GTTCCCTCCC CTACGGCACA GCCATGGAAA AAGCTCAGCT CAAGCCTCCA 
GCCACCTCAG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itga5 ( NM_010577.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034707.5 Mus musculus integrin alpha 5 (fibronectin receptor alpha) (Itga5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22491
Clone ID Related Accession (Same CDS sequence)	NM_010577.4
Accession Version	NM_010577.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	integrin alpha-5 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC131721.4, BC050943.1 and AK142241.1. On Sep 26, 2015 this sequence version replaced NM_010577.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050943.1, AK142241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGCT GGACGCCACG GTCGCCTCGA TCTCCTCTCC ACGCGGTGCT GCTGCGCTGG 
GGGCCCCGAC GCCTACCGCC GCTGCTGCCT CTGCTGCTGC TACTGTGGCC GCCACCACTC 
CAGGTTGGGG GCTTCAACCT AGACGCGGAG GCCCCGGCGG TGCTCTCCGG GCCCCCCGGC 
TCCCTTTTTG GCTTCTCCGT GGAGTTTTAC CGGCCGGGAA GGGACGGAGT CAGTGTGCTG 
GTTGGGGCAC CCAAGGCTAA CACTAGCCAG CCAGGTGTAC TGCAAGGTGG TGCTGTCTAT 
GTGTGTCCCT GGGGCACCAG TCCTATCCAG TGCACCACCA TTCAATTTGA CAGCAAAGGC 
TCCCGGATTC TGGAGTCCTC ACTGTACAGT GCCAAGGGAG AGGAGCCTGT GGAGTACAAG 
TCCTTGCAGT GGTTCGGAGC AACAGTTCGG GCCCATGGCT CCTCCATCTT GGCATGTGCT 
CCACTGTATA GCTGGCGCAC AGAAAAGGAC CCACAGAATG ACCCAGTGGG CACCTGCTAC 
CTCTCCACAG AAAACTTCAC CCGGATTCTG GAGTACGCAC CTTGCCGCTC AGATTTTGGC 
AGTGCAGCAG GGCAGGGCTA CTGCCAAGGG GGCTTCAGTG CTGAGTTCAC CAAGACTGGC 
CGTGTGGTCC TGGGTGGACC TGGAAGCTAC TTCTGGCAAG GCCAGATCCT GTCCGCCACT 
CAAGAGCAGA TCTCGGAGTC CTATTACCCA GAGTATCTCA TCAACCCTGT TCAGGGGCAG 
CTGCAGACCC GCCAGGCCAG CTCCGTCTAT GATGACAGCT ACTTGGGATA CTCTGTGGCT 
GTGGGTGAAT TCAGTGGTGA TGACACAGAA GACTTTGTTG CTGGCGTGCC CAAGGGGAAC 
CTCACCTATG GCTATGTCAC CGTCCTTAAT GGCTCAGACA TCCACTCCCT CTACAACGTC 
TCAGGAGAAC AGATGGCCTC CTACTTCGGC TATGCTGTGG CTGCCACTGA TACCAATGGA 
GATGGGCTAG ATGACCTACT GGTAGGGGCA CCCCTGCTCA TGGAGCGGAC AGCTGATGGG 
AGACCTCAGG AGGTGGGCAG GGTCTACATC TATCTGCAGC GCCCAGCGGG CATAGATCCC 
ACACCCACCC TCACCCTCAC TGGGCAAGAT GAGTTCAGCC GATTCGGCAG CTCCTTGACA 
CCCTTGGGGG ACCTGGACCA AGACGGCTAC AATGATGTCG CCATTGGGGC TCCATTTGGT 
GGGGAGGCCC AGCAGGGAGT CGTATTTATA TTCCCGGGAG GCCCAGGAGG ACTGAGCACT 
AAACCTTCCC AGGTTTTGCA GCCTCTTTGG GCAGCTGGCC GTACCCCAGA CTTCTTTGGC 
TCTGCCCTTC GAGGAGGACG AGATCTGGAT GGCAATGGAT ACCCTGATCT AATCGTTGGA 
TCCTTTGGTG TGGACAAGGC TCTGGTGTAC AGAGGGCGGC CCATCATATC TGCCAGCGCA 
TCTCTCACCA TCTTCCCCTC CATGTTCAAC CCAGAGGAGC GCAGTTGCAG CCTGGAAGGG 
AACCCTGTGT CCTGCATCAA CCTTAGCTTC TGCCTCAATG CCTCTGGAAA ACATGTCCCC 
AACTCTATAG GCTTCGAGGT GGAACTCCAA CTGGACTGGC AGAAGCAAAA GGGAGGGGTC 
CGGCGGGCAC TGTTCCTGAC TTCCAAGCAG GCCACCCTTA CCCAGACCCT GCTTATCCAG 
AATGGGGCTC GGGAGGACTG CAGGGAGATG AAGATCTACC TCAGGAATGA ATCAGAATTC 
AGAGACAAAC TCTCCCCAAT TCACATTGCC CTCAACTTCT CCTTGGACCC CAAAGCTCCC 
ATGGACAGCC ATGGCCTCCG GCCAGTTCTA CACTACCAAA GCAAAAGCAG GATAGAGGAC 
AAGGCCCAGA TCTTGCTGGA CTGTGGTGAA GACAATATCT GTGTGCCTGA CCTGCAGCTG 
GATGTGTATG GGGAGAAGAA ACATGTGTAC CTGGGTGACA AGAACGCACT GAACCTGACA 
TTCCATGCCC AAAATCTGGG TGAGGGCGGT GCCTATGAAG CCGAGCTTCG GGTCACAGCC 
CCTCTAGAGG CCGAGTACTC AGGACTTGTC AGACACCCAG GGAACTTCTC CAGCCTGAGC 
TGTGACTACT TTGCTGTGAA CCAGAGCCGC CAGCTGGTGT GTGACCTGGG CAACCCCATG 
AAGGCAGGCA CCAGTCTCTG GGGTGGCCTT CGGTTCACTG TTCCTCATCT TCAAGACACA 
AAGAAAACCA TCCAGTTTGA CTTTCAGATC CTCAGCAAGA ACCTGAACAA CTCACAAAGC 
AACGTGGTCT CCTTCCCACT CTCGGTGGAG GCTCAAGCCC AGGTCTCCCT TAATGGTGTC 
TCCAAGCCTG AAGCTGTGAT TTTCCCAGTC AGCGACTGGA ATCCTCAAGA CCAGCCTCAG 
AAGGAGGAAG ACTTGGGCCC AGCTGTCCAC CATGTCTACG AGCTCATCAA CCAGGGGCCC 
AGCTCCATCA GCCAGGGTGT GCTGGAGCTC AGCTGTCCAC AGGCTCTGGA AGGCCAACAG 
CTCCTCTATG TGACCAAGGT GACAGGACTC AGCAACTGCA CCTCCAACTA CACCCCCAAC 
TCACAGGGCC TGGAGTTGGA TCCAGAGACC TCTCCACACC ACCTGCAGAA ACGAGAGGCT 
CCAGGGAGGA GTTCTACTGC CTCAGGAACA CAAGTTCTGA AATGCCCTGA AGCCAAGTGT 
TTCAGGCTGC GCTGTGAGTT TGGGCCACTG CACCGCCAAG AGAGCCGTAG TCTGCAGCTG 
CATTTCCGAG TCTGGGCCAA GACCTTCTTG CAGCGGGAAT ACCAGCCATT TAGCCTTCAG 
TGTGAGGCTG TATATGAAGC TCTGAAGATG CCCTACCAGA TCCTGCCTCG GCAGCTTCCC 
CAAAAGAAAC TTCAGGTGGC CACAGCCGTG CAGTGGACCA AGGCAGAAGG CAGCAATGGT 
GTCCCGTTGT GGATCATCAT CCTAGCCATT CTTTTTGGCC TCCTGCTCCT AGGTCTGCTC 
ATCTACGTCC TCTACAAGCT CGGCTTCTTC AAACGTTCCC TCCCCTACGG CACAGCCATG 
GAAAAAGCTC AGCTCAAGCC TCCAGCCACC TCAGATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034707.5
CDS	266..3427
Translation	MGSWTPRSPRSPLHAVLLRWGPRRLPPLLPLLLLLWPPPLQVGGFNLDAEAPAVLSGPPGSLFGFSVEFYRPGRDGVSVLVGAPKANTSQPGVLQGGAVYVCPWGTSPIQCTTIQFDSKGSRILESSLYSAKGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKDPQNDPVGTCYLSTENFTRILEYAPCRSDFGSAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQISESYYPEYLINPVQGQLQTRQASSVYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTVLNGSDIHSLYNVSGEQMASYFGYAVAATDTNGDGLDDLLVGAPLLMERTADGRPQEVGRVYIYLQRPAGIDPTPTLTLTGQDEFSRFGSSLTPLGDLDQDGYNDVAIGAPFGGEAQQGVVFIFPGGPGGLSTKPSQVLQPLWAAGRTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKALVYRGRPIISASASLTIFPSMFNPEERSCSLEGNPVSCINLSFCLNASGKHVPNSIGFEVELQLDWQKQKGGVRRALFLTSKQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPKAPMDSHGLRPVLHYQSKSRIEDKAQILLDCGEDNICVPDLQLDVYGEKKHVYLGDKNALNLTFHAQNLGEGGAYEAELRVTAPLEAEYSGLVRHPGNFSSLSCDYFAVNQSRQLVCDLGNPMKAGTSLWGGLRFTVPHLQDTKKTIQFDFQILSKNLNNSQSNVVSFPLSVEAQAQVSLNGVSKPEAVIFPVSDWNPQDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTKVTGLSNCTSNYTPNSQGLELDPETSPHHLQKREAPGRSSTASGTQVLKCPEAKCFRLRCEFGPLHRQESRSLQLHFRVWAKTFLQREYQPFSLQCEAVYEALKMPYQILPRQLPQKKLQVATAVQWTKAEGSNGVPLWIIILAILFGLLLLGLLIYVLYKLGFFKRSLPYGTAMEKAQLKPPATSDA

Target ORF information:

RefSeq Version	NM_010577.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin alpha 5 (fibronectin receptor alpha) (Itga5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010577.4

ORF Insert Sequence:

ATGGGGAGCT GGACGCCACG GTCGCCTCGA TCTCCTCTCC ACGCGGTGCT GCTGCGCTGG 
GGGCCCCGAC GCCTACCGCC GCTGCTGCCT CTGCTGCTGC TACTGTGGCC GCCACCACTC 
CAGGTTGGGG GCTTCAACCT AGACGCGGAG GCCCCGGCGG TGCTCTCCGG GCCCCCCGGC 
TCCCTTTTTG GCTTCTCCGT GGAGTTTTAC CGGCCGGGAA GGGACGGAGT CAGTGTGCTG 
GTTGGGGCAC CCAAGGCTAA CACTAGCCAG CCAGGTGTAC TGCAAGGTGG TGCTGTCTAT 
GTGTGTCCCT GGGGCACCAG TCCTATCCAG TGCACCACCA TTCAATTTGA CAGCAAAGGC 
TCCCGGATTC TGGAGTCCTC ACTGTACAGT GCCAAGGGAG AGGAGCCTGT GGAGTACAAG 
TCCTTGCAGT GGTTCGGAGC AACAGTTCGG GCCCATGGCT CCTCCATCTT GGCATGTGCT 
CCACTGTATA GCTGGCGCAC AGAAAAGGAC CCACAGAATG ACCCAGTGGG CACCTGCTAC 
CTCTCCACAG AAAACTTCAC CCGGATTCTG GAGTACGCAC CTTGCCGCTC AGATTTTGGC 
AGTGCAGCAG GGCAGGGCTA CTGCCAAGGG GGCTTCAGTG CTGAGTTCAC CAAGACTGGC 
CGTGTGGTCC TGGGTGGACC TGGAAGCTAC TTCTGGCAAG GCCAGATCCT GTCCGCCACT 
CAAGAGCAGA TCTCGGAGTC CTATTACCCA GAGTATCTCA TCAACCCTGT TCAGGGGCAG 
CTGCAGACCC GCCAGGCCAG CTCCGTCTAT GATGACAGCT ACTTGGGATA CTCTGTGGCT 
GTGGGTGAAT TCAGTGGTGA TGACACAGAA GACTTTGTTG CTGGCGTGCC CAAGGGGAAC 
CTCACCTATG GCTATGTCAC CGTCCTTAAT GGCTCAGACA TCCACTCCCT CTACAACGTC 
TCAGGAGAAC AGATGGCCTC CTACTTCGGC TATGCTGTGG CTGCCACTGA TACCAATGGA 
GATGGGCTAG ATGACCTACT GGTAGGGGCA CCCCTGCTCA TGGAGCGGAC AGCTGATGGG 
AGACCTCAGG AGGTGGGCAG GGTCTACATC TATCTGCAGC GCCCAGCGGG CATAGATCCC 
ACACCCACCC TCACCCTCAC TGGGCAAGAT GAGTTCAGCC GATTCGGCAG CTCCTTGACA 
CCCTTGGGGG ACCTGGACCA AGACGGCTAC AATGATGTCG CCATTGGGGC TCCATTTGGT 
GGGGAGGCCC AGCAGGGAGT CGTATTTATA TTCCCGGGAG GCCCAGGAGG ACTGAGCACT 
AAACCTTCCC AGGTTTTGCA GCCTCTTTGG GCAGCTGGCC GTACCCCAGA CTTCTTTGGC 
TCTGCCCTTC GAGGAGGACG AGATCTGGAT GGCAATGGAT ACCCTGATCT AATCGTTGGA 
TCCTTTGGTG TGGACAAGGC TCTGGTGTAC AGAGGGCGGC CCATCATATC TGCCAGCGCA 
TCTCTCACCA TCTTCCCCTC CATGTTCAAC CCAGAGGAGC GCAGTTGCAG CCTGGAAGGG 
AACCCTGTGT CCTGCATCAA CCTTAGCTTC TGCCTCAATG CCTCTGGAAA ACATGTCCCC 
AACTCTATAG GCTTCGAGGT GGAACTCCAA CTGGACTGGC AGAAGCAAAA GGGAGGGGTC 
CGGCGGGCAC TGTTCCTGAC TTCCAAGCAG GCCACCCTTA CCCAGACCCT GCTTATCCAG 
AATGGGGCTC GGGAGGACTG CAGGGAGATG AAGATCTACC TCAGGAATGA ATCAGAATTC 
AGAGACAAAC TCTCCCCAAT TCACATTGCC CTCAACTTCT CCTTGGACCC CAAAGCTCCC 
ATGGACAGCC ATGGCCTCCG GCCAGTTCTA CACTACCAAA GCAAAAGCAG GATAGAGGAC 
AAGGCCCAGA TCTTGCTGGA CTGTGGTGAA GACAATATCT GTGTGCCTGA CCTGCAGCTG 
GATGTGTATG GGGAGAAGAA ACATGTGTAC CTGGGTGACA AGAACGCACT GAACCTGACA 
TTCCATGCCC AAAATCTGGG TGAGGGCGGT GCCTATGAAG CCGAGCTTCG GGTCACAGCC 
CCTCTAGAGG CCGAGTACTC AGGACTTGTC AGACACCCAG GGAACTTCTC CAGCCTGAGC 
TGTGACTACT TTGCTGTGAA CCAGAGCCGC CAGCTGGTGT GTGACCTGGG CAACCCCATG 
AAGGCAGGCA CCAGTCTCTG GGGTGGCCTT CGGTTCACTG TTCCTCATCT TCAAGACACA 
AAGAAAACCA TCCAGTTTGA CTTTCAGATC CTCAGCAAGA ACCTGAACAA CTCACAAAGC 
AACGTGGTCT CCTTCCCACT CTCGGTGGAG GCTCAAGCCC AGGTCTCCCT TAATGGTGTC 
TCCAAGCCTG AAGCTGTGAT TTTCCCAGTC AGCGACTGGA ATCCTCAAGA CCAGCCTCAG 
AAGGAGGAAG ACTTGGGCCC AGCTGTCCAC CATGTCTACG AGCTCATCAA CCAGGGGCCC 
AGCTCCATCA GCCAGGGTGT GCTGGAGCTC AGCTGTCCAC AGGCTCTGGA AGGCCAACAG 
CTCCTCTATG TGACCAAGGT GACAGGACTC AGCAACTGCA CCTCCAACTA CACCCCCAAC 
TCACAGGGCC TGGAGTTGGA TCCAGAGACC TCTCCACACC ACCTGCAGAA ACGAGAGGCT 
CCAGGGAGGA GTTCTACTGC CTCAGGAACA CAAGTTCTGA AATGCCCTGA AGCCAAGTGT 
TTCAGGCTGC GCTGTGAGTT TGGGCCACTG CACCGCCAAG AGAGCCGTAG TCTGCAGCTG 
CATTTCCGAG TCTGGGCCAA GACCTTCTTG CAGCGGGAAT ACCAGCCATT TAGCCTTCAG 
TGTGAGGCTG TATATGAAGC TCTGAAGATG CCCTACCAGA TCCTGCCTCG GCAGCTTCCC 
CAAAAGAAAC TTCAGGTGGC CACAGCCGTG CAGTGGACCA AGGCAGAAGG CAGCAATGGT 
GTCCCGTTGT GGATCATCAT CCTAGCCATT CTTTTTGGCC TCCTGCTCCT AGGTCTGCTC 
ATCTACGTCC TCTACAAGCT CGGCTTCTTC AAACGTTCCC TCCCCTACGG CACAGCCATG 
GAAAAAGCTC AGCTCAAGCC TCCAGCCACC TCAGATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Itga5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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