Ins2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ins2
Entrez Gene ID	16334
Full Name	insulin II
Synonyms	AA986540,Ins-2,InsII,Mody,Mody4
General protein information	Preferred Names insulin II Names insulin-2 proinsulin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 F5\|7 88.0 cM
Summary	This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001185084.2	NP_001172013.1	insulin-2 preproprotein
NM_008387.5	NP_032413.1	insulin-2 preproprotein
NM_001185083.2	NP_001172012.1	insulin-2 preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	INS	NP_001008996.1
Mus musculus (house mouse)	Ins2	NP_032413.1
Gallus gallus (chicken)	INS	NP_990553.1
Danio rerio (zebrafish)	ins	NP_571131.1
Xenopus tropicalis (tropical clawed frog)	ins	NP_001093706.1
Rattus norvegicus (Norway rat)	Ins2	NP_062003.1
Canis lupus familiaris (dog)	INS	NP_001123565.1
Homo sapiens (human)	INS	NP_000198.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel splice variant of mouse insulin2 mRNA: implications for insulin expression.
Panda AC, Kulkarni SD, Muralidharan B, Bakthavachalu B, Seshadri V
FEBS letters584(6)1169-73(2010 Mar)

Increased insulin translation from an insulin splice-variant overexpressed in diabetes, obesity, and insulin resistance.
Minn AH, Lan H, Rabaglia ME, Harlan DM, Peculis BA, Attie AD, Shalev A
Molecular endocrinology (Baltimore, Md.)19(3)794-803(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cTAGE5 deletion in pancreatic beta cells impairs proinsulin trafficking and insulin biogenesis in mice.

These results suggest that prolonged exposure to hyperglycemia in the Ins2(Akita+/-) mice leads to progressive testicular disruption mediated by testicular activin activity, rather than hormonal dysregulation.
Title: Hyperglycemia is associated with reduced testicular function and activin dysregulation in the Ins2

Data suggest that resveratrol acts on differentiating preadipocytes by inhibiting insulin signaling, mitochondrial biogenesis, and lipogenesis.
Title: Resveratrol inhibits lipogenesis of 3T3-L1 and SGBS cells by inhibition of insulin signaling and mitochondrial mass increase.

Wnt3a increased the expression of NeuroD1 and Ins2 in the hypothalamus.
Title: Wnt3a upregulates brain-derived insulin by increasing NeuroD1 via Wnt/?-catenin signaling in the hypothalamus.

report that EndMTs occur in the diabetic endothelium of Ins2Akita/wt mouse, and show that induction of sex determining region Y-box 2 (Sox2) is a mediator of excess BMP signaling that results in activation of EndMTs and increased vascular calcification
Title: Endothelial-Mesenchymal Transition in Vascular Calcification of Ins2Akita/+ Mice.

The following Ins2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ins2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22987	NM_001185083.2 Latest version!	Mus musculus insulin II (Ins2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu22987	NM_008387.5 Latest version!	Mus musculus insulin II (Ins2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu22987	NM_001185084.2 Latest version!	Mus musculus insulin II (Ins2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ins2 ( NM_001185084.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001172013.1 Mus musculus insulin II (Ins2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu22987
Clone ID Related Accession (Same CDS sequence)	NM_001185084.2 , NM_008387.5 , NM_001185083.2
Accession Version	NM_001185084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	insulin-2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1, AY899305.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced NM_001185084.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP775682.1, AY899305.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC 
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC 
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG 
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172013.1
CDS	194..526
Translation	MALWMRFLPLLALLFLWESHPTQAFVKQHLCGSHLVEALYLVCGERGFFYTPMSRREVEDPQVAQLELGGGPGAGDLQTLALEVAQQKRGIVDQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_001185084.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus insulin II (Ins2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185084.2

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC 
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC 
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG 
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ins2 ( NM_008387.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032413.1 Mus musculus insulin II (Ins2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22987
Clone ID Related Accession (Same CDS sequence)	NM_001185084.2 , NM_008387.5 , NM_001185083.2
Accession Version	NM_008387.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	insulin-2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1, AK007612.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced NM_008387.4. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP775562.1, AK007612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC 
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC 
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG 
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032413.1
CDS	92..424
Translation	MALWMRFLPLLALLFLWESHPTQAFVKQHLCGSHLVEALYLVCGERGFFYTPMSRREVEDPQVAQLELGGGPGAGDLQTLALEVAQQKRGIVDQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_008387.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus insulin II (Ins2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008387.5

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC 
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC 
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG 
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ins2 ( NM_001185083.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001172012.1 Mus musculus insulin II (Ins2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22987
Clone ID Related Accession (Same CDS sequence)	NM_001185084.2 , NM_008387.5 , NM_001185083.2
Accession Version	NM_001185083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	insulin-2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced NM_001185083.1. Transcript Variant: This variant (1) represents the shortest transcript. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP775727.1, BG310959.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC 
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC 
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG 
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172012.1
CDS	80..412
Translation	MALWMRFLPLLALLFLWESHPTQAFVKQHLCGSHLVEALYLVCGERGFFYTPMSRREVEDPQVAQLELGGGPGAGDLQTLALEVAQQKRGIVDQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_001185083.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus insulin II (Ins2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185083.2

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC 
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC 
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG 
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel splice variant of mouse insulin2 mRNA: implications for insulin expression.
FEBS letters584(6)1169-73(2010 Mar)
Panda AC,Kulkarni SD,Muralidharan B,Bakthavachalu B,Seshadri V

Increased insulin translation from an insulin splice-variant overexpressed in diabetes, obesity, and insulin resistance.
Molecular endocrinology (Baltimore, Md.)19(3)794-803(2005 Mar)
Minn AH,Lan H,Rabaglia ME,Harlan DM,Peculis BA,Attie AD,Shalev A

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Ins2 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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