Dnajb3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnajb3
Entrez Gene ID	15504
Full Name	DnaJ heat shock protein family (Hsp40) member B3
Synonyms	Hsj3,MSJ-1,Msj1
General protein information	Preferred Names DnaJ heat shock protein family (Hsp40) member B3 Names dnaJ homolog subfamily B member 3 DnaJ (Hsp40) homolog, subfamily B, member 3 HSJ-3 dnaJ protein homolog 3 heat shock protein J3 heat shock protein, DNAJ-like 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 D

mRNA and Protein(s)

mRNA	Protein	Name
NM_008299.3	NP_032325.2	dnaJ homolog subfamily B member 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC610356	XP_005635931.1
Rattus norvegicus (Norway rat)	Dnajb3	NP_001102866.1
Bos taurus (cattle)	DNAJB3	NP_001069384.1
Mus musculus (house mouse)	Dnajb3	NP_032325.2

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Chaperone protein HYPK interacts with the first 17 amino acid region of Huntingtin and modulates mutant HTT-mediated aggregation and cytotoxicity.
Choudhury KR, Bhattacharyya NP
Biochemical and biophysical research communications456(1)66-73(2015 Jan)

Structure of msj-1 gene in mice and humans: a possible role in the regulation of male reproduction.
Meccariello R, Berruti G, Chianese R, De Santis R, Di Cunto F, Scarpa D, Cobellis G, Zucchetti I, Pierantoni R, Altruda F, Fasano S
General and comparative endocrinology156(1)91-103(2008 Mar)

The complexity of antisense transcription revealed by the study of developing male germ cells.
Chan WY, Wu SM, Ruszczyk L, Law E, Lee TL, Baxendale V, Lap-Yin Pang A, Rennert OM
Genomics87(6)681-92(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

msj-1 gene might be conserved among vertebrates and might exert fundamental functions in reproduction.
Title: Structure of msj-1 gene in mice and humans: a possible role in the regulation of male reproduction.

Analysis of msj-1 genomic sequence reveals that msj-1 is an intronless gene.
Title: Structure of msj-1 gene: a comparative analysis.

a molecular chaperone, specifically expressed in testis, associates with the acrosome and centrosome in mouse germ cells
Title: mUBPy and MSJ-1, a deubiquitinating enzyme and a molecular chaperone specifically expressed in testis, associate with the acrosome and centrosome in mouse germ cells.

The following Dnajb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnajb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58420	NM_008299.3 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member B3 (Dnajb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnajb3 ( NM_008299.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032325.2 Mus musculus DnaJ heat shock protein family (Hsp40) member B3 (Dnajb3), mRNA.

Price & Availability↑

CloneID	OMu58420
Clone ID Related Accession (Same CDS sequence)	NM_008299.3
Accession Version	NM_008299.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily B member 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U95607.1 and AK005690.1. On Apr 11, 2009 this sequence version replaced NM_008299.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: U95607.1 [ECO:0000345] ##Evidence-Data-END##

ATGGTGGACT ACTACGAGGT GCTGGGCGTG CCGCGGCAGG CCTCAGCCGA GGCCATCCGC 
AAGGCGTACC GCAAGCTTGC GCTCAAGTGG CACCCGGACA AGAACCCCGA GCACAAGGAG 
GAGGCCGAGA GGCGGTTCAA GCAGGTGGCG CAGGCCTACG AGGTCTTATC GGACGTCCGC 
AAGCGCGAGG TGTACGACCG CTGCGGCGAA GTGGGCGAGG TGGGCGGAGG CGGCGCGGCG 
GGCAGCCCGT TCCACGATGC CTTCCAGTAC GTCTTCTCCT TCCGAGACCC CGCGGAGGTC 
TTCAGGGAGT TCTTCGGGGG CCACGACCCT TTCTCCTTCG ACTTCTTCGG GGGAGACCCC 
CTTGAGAACT TTTTTGGGGA TCGGAGGAGC ACCCGTGGAA GCAGAAGCCG AGGGGCTGTA 
CCCTTCTCTA CCTCCTTTAC CGAATTCCCA GGATTTGGGG GTGGCTTCGC TTCGTTAGAT 
ACTGGATTCA CATCCTTCGG TTCTCCAGGA AACTCGGGCC TTTCTTCTTT CTCCATGTCT 
TGCGGCGGTG GGGCGGCAGG CAACTACAAG TCGGTGTCAA CCTCCACCGA AATAATTAAT 
GGCAAAAAAA TCACCACCAA GAGAATCGTT GAGAATGGTC AAGAAAGGGT GGAAGTGGAA 
GAAGATGGAG AGTTAAAGTC CCTGATAATT AATGGCAAAG AGCAGCTGCT TCGCATCAAT 
ACTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032325.2
CDS	71..799
Translation	MVDYYEVLGVPRQASAEAIRKAYRKLALKWHPDKNPEHKEEAERRFKQVAQAYEVLSDVRKREVYDRCGEVGEVGGGGAAGSPFHDAFQYVFSFRDPAEVFREFFGGHDPFSFDFFGGDPLENFFGDRRSTRGSRSRGAVPFSTSFTEFPGFGGGFASLDTGFTSFGSPGNSGLSSFSMSCGGGAAGNYKSVSTSTEIINGKKITTKRIVENGQERVEVEEDGELKSLIINGKEQLLRINTQ

Target ORF information:

RefSeq Version	NM_008299.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member B3 (Dnajb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008299.3

ORF Insert Sequence:

ATGGTGGACT ACTACGAGGT GCTGGGCGTG CCGCGGCAGG CCTCAGCCGA GGCCATCCGC 
AAGGCGTACC GCAAGCTTGC GCTCAAGTGG CACCCGGACA AGAACCCCGA GCACAAGGAG 
GAGGCCGAGA GGCGGTTCAA GCAGGTGGCG CAGGCCTACG AGGTCTTATC GGACGTCCGC 
AAGCGCGAGG TGTACGACCG CTGCGGCGAA GTGGGCGAGG TGGGCGGAGG CGGCGCGGCG 
GGCAGCCCGT TCCACGATGC CTTCCAGTAC GTCTTCTCCT TCCGAGACCC CGCGGAGGTC 
TTCAGGGAGT TCTTCGGGGG CCACGACCCT TTCTCCTTCG ACTTCTTCGG GGGAGACCCC 
CTTGAGAACT TTTTTGGGGA TCGGAGGAGC ACCCGTGGAA GCAGAAGCCG AGGGGCTGTA 
CCCTTCTCTA CCTCCTTTAC CGAATTCCCA GGATTTGGGG GTGGCTTCGC TTCGTTAGAT 
ACTGGATTCA CATCCTTCGG TTCTCCAGGA AACTCGGGCC TTTCTTCTTT CTCCATGTCT 
TGCGGCGGTG GGGCGGCAGG CAACTACAAG TCGGTGTCAA CCTCCACCGA AATAATTAAT 
GGCAAAAAAA TCACCACCAA GAGAATCGTT GAGAATGGTC AAGAAAGGGT GGAAGTGGAA 
GAAGATGGAG AGTTAAAGTC CCTGATAATT AATGGCAAAG AGCAGCTGCT TCGCATCAAT 
ACTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Chaperone protein HYPK interacts with the first 17 amino acid region of Huntingtin and modulates mutant HTT-mediated aggregation and cytotoxicity.
Biochemical and biophysical research communications456(1)66-73(2015 Jan)
Choudhury KR,Bhattacharyya NP

Structure of msj-1 gene in mice and humans: a possible role in the regulation of male reproduction.
General and comparative endocrinology156(1)91-103(2008 Mar)
Meccariello R,Berruti G,Chianese R,De Santis R,Di Cunto F,Scarpa D,Cobellis G,Zucchetti I,Pierantoni R,Altruda F,Fasano S

The complexity of antisense transcription revealed by the study of developing male germ cells.
Genomics87(6)681-92(2006 Jun)
Chan WY,Wu SM,Ruszczyk L,Law E,Lee TL,Baxendale V,Lap-Yin Pang A,Rennert OM

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Dnajb3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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