Hsd3b6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsd3b6
Entrez Gene ID	15497
Full Name	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6
Synonyms	D19210
General protein information	Preferred Names hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6 Names 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type VI 3-beta-HSD VI 3beta-HSD VI hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6 pseudogene hydroxysteroid dehydrogenase-6, delta<5>-3-beta
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_013821.3	NP_038849.2	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6

Pathways from BioSystems

Homologies

Homo sapiens (human)	HSD3B1	NP_000853.1
Pan troglodytes (chimpanzee)	HSD3B1	XP_001141854.1
Mus musculus (house mouse)	Hsd3b3	NP_001155214.1
	HSD3B1	XP_001113873.1
Mus musculus (house mouse)	Hsd3b2	NP_694873.2
Mus musculus (house mouse)	Hsd3b6	NP_038849.2

Gene Ontology
Bibliography

Related articles in PubMed

The eutheria-specific miR-290 cluster modulates placental growth and maternal-fetal transport.
Paikari A, D Belair C, Saw D, Blelloch R
Development (Cambridge, England)144(20)3731-3743(2017 10)

Neuregulin 1 Regulates Proliferation of Leydig Cells to Support Spermatogenesis and Sexual Behavior in Adult Mice.
Umehara T, Kawashima I, Kawai T, Hoshino Y, Morohashi KI, Shima Y, Zeng W, Richards JS, Shimada M
Endocrinology157(12)4899-4913(2016 Dec)

A potential role for zinc transporter 7 in testosterone synthesis in mouse Leydig tumor cells.
Chu Q, Chi ZH, Zhang X, Liang D, Wang X, Zhao Y, Zhang L, Zhang P
International journal of molecular medicine37(6)1619-26(2016 Jun)

Steroidogenic factor 1 differentially regulates fetal and adult leydig cell development in male mice.
Karpova T, Ravichandiran K, Insisienmay L, Rice D, Agbor V, Heckert LL
Biology of reproduction93(4)83(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results demonstrated that ZnT7 gene silencing downregulated the expression of StAR, P450scc and 3beta-HSD as well as progesterone concentrations in the human chorionic gonadotrophin-stimulated Leydig tumor cells.
Title: A potential role for zinc transporter 7 in testosterone synthesis in mouse Leydig tumor cells.

Hsd3b6 is localized in multiple specific tissues and cell types.
Title: Immunolocalization of murine type VI 3?-hydroxysteroid dehydrogenase in the adrenal gland, testis, skin, and placenta.

a novel 3beta-hydroxyl-steroid dehydrogenase (3beta-Hsd) gene under clock control is severely overexpressed specifically in aldosterone-producing cells in the adrenal cortex, leading to hyperaldosteronism and ultimately to salt-sensitive hypertension
Title: Hypertension due to loss of clock: novel insight from the molecular analysis of Cry1/Cry2-deleted mice.

with Cry gene inactivation results in dyregulation, and abnormally high plasma aldosterone concentration and salt-sensitive hypertension
Title: Salt-sensitive hypertension in circadian clock-deficient Cry-null mice involves dysregulated adrenal Hsd3b6.

The following Hsd3b6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsd3b6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10016	NM_013821.3 Latest version!	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6 (Hsd3b6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsd3b6 ( NM_013821.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038849.2 Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6 (Hsd3b6), mRNA.

Price & Availability↑

CloneID	OMu10016
Clone ID Related Accession (Same CDS sequence)	NM_013821.3
Accession Version	NM_013821.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK142267.1 and BC053501.1. On Dec 14, 2007 this sequence version replaced NM_013821.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053501.1, AK142267.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGGT GGAGCTGCCT GGTAACTGGA GCAGGAGGGT TTTTGGGCCA GAGGATTGTC 
CAGTTGTTGA TGCAGGAGAA AGATCTGGAG GAGATCAGGG TCCTGGACAA GTTCTTCAGA 
CCAGAAACCA GGGAGCAATT CTTCAACCTA GATACAAACA TCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TTGACACCCA GTACCTGAGG AAAGCCTGCC AGGGCATCTC TGTTGTTATC 
CACACTGCAG CTGTCATTGA TGTCACAGGT GTCATTCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CTTATTGGAG GCCTGTATCC AAGCCAGTGT TCCAGCCTTC 
ATCTTCTCCA GCTCAGTTGA TGTTGCAGGG CCCAACTCAT ACAAGGAGAT AATCCTGAAT 
GGCAACGAGG AAGAGCATCA TGAAAGCATA TGGTCTGATC CATACCCATA CAGCAAAAAG 
ATGGCTGAGA AGGCAGTGCT GGCAGCCAAT GGGAGCATGC TGAAAATTGG TGGCACTTTG 
CATACTTGTG CATTAAGGCC CATGTATATT TATGGGGAGA GAAGTCCATT CATTTCTAAC 
ACAATAATTA CGGCCCTCAA AAATAAGAAT ATTCTCGGTT GTACGGGCAA ATTCTCCACA 
GCCAACCCAG TATATGTGGG TAATGTAGCC TGGGCACACA TTCTGGCTGC CAGGGGCCTT 
CGAGACCCCA AAAAGTCACC AAATATCCAA GGAGAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAC ACCCTGAGCA AGGAGTGGGG CTTCTGCCCT 
GATTCCAGCT GGAGCCTTCC TGTGCCCCTA CTGTACTGGC TTGCATTCAT GCTGGAAACT 
GTGAGCTTCC TGCTGAGTCC AATCTACAGA TTTATACCTC CCTTTAACCG CCACTTGGTC 
ACACTGACAG GTAGCACGTT CACTTTCTCC TACAAGAAAG CTCAGCGAGA TCTGGGCTAT 
GAGCCACTTG TCAGCTGGGA GGAAGCCAAG CAGAAAACCT CAGAGTGGAT CGGGACACTA 
GTGGAGCAGC ACAGGGAGAC ACTGGACACA AAGTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038849.2
CDS	325..1446
Translation	MPGWSCLVTGAGGFLGQRIVQLLMQEKDLEEIRVLDKFFRPETREQFFNLDTNIKVTVLEGDILDTQYLRKACQGISVVIHTAAVIDVTGVIPRQTILDVNLKGTQNLLEACIQASVPAFIFSSSVDVAGPNSYKEIILNGNEEEHHESIWSDPYPYSKKMAEKAVLAANGSMLKIGGTLHTCALRPMYIYGERSPFISNTIITALKNKNILGCTGKFSTANPVYVGNVAWAHILAARGLRDPKKSPNIQGEFYYISDDTPHQSYDDLNYTLSKEWGFCPDSSWSLPVPLLYWLAFMLETVSFLLSPIYRFIPPFNRHLVTLTGSTFTFSYKKAQRDLGYEPLVSWEEAKQKTSEWIGTLVEQHRETLDTKSQ

Target ORF information:

RefSeq Version	NM_013821.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6 (Hsd3b6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013821.3

ORF Insert Sequence:

ATGCCTGGGT GGAGCTGCCT GGTAACTGGA GCAGGAGGGT TTTTGGGCCA GAGGATTGTC 
CAGTTGTTGA TGCAGGAGAA AGATCTGGAG GAGATCAGGG TCCTGGACAA GTTCTTCAGA 
CCAGAAACCA GGGAGCAATT CTTCAACCTA GATACAAACA TCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TTGACACCCA GTACCTGAGG AAAGCCTGCC AGGGCATCTC TGTTGTTATC 
CACACTGCAG CTGTCATTGA TGTCACAGGT GTCATTCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CTTATTGGAG GCCTGTATCC AAGCCAGTGT TCCAGCCTTC 
ATCTTCTCCA GCTCAGTTGA TGTTGCAGGG CCCAACTCAT ACAAGGAGAT AATCCTGAAT 
GGCAACGAGG AAGAGCATCA TGAAAGCATA TGGTCTGATC CATACCCATA CAGCAAAAAG 
ATGGCTGAGA AGGCAGTGCT GGCAGCCAAT GGGAGCATGC TGAAAATTGG TGGCACTTTG 
CATACTTGTG CATTAAGGCC CATGTATATT TATGGGGAGA GAAGTCCATT CATTTCTAAC 
ACAATAATTA CGGCCCTCAA AAATAAGAAT ATTCTCGGTT GTACGGGCAA ATTCTCCACA 
GCCAACCCAG TATATGTGGG TAATGTAGCC TGGGCACACA TTCTGGCTGC CAGGGGCCTT 
CGAGACCCCA AAAAGTCACC AAATATCCAA GGAGAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAC ACCCTGAGCA AGGAGTGGGG CTTCTGCCCT 
GATTCCAGCT GGAGCCTTCC TGTGCCCCTA CTGTACTGGC TTGCATTCAT GCTGGAAACT 
GTGAGCTTCC TGCTGAGTCC AATCTACAGA TTTATACCTC CCTTTAACCG CCACTTGGTC 
ACACTGACAG GTAGCACGTT CACTTTCTCC TACAAGAAAG CTCAGCGAGA TCTGGGCTAT 
GAGCCACTTG TCAGCTGGGA GGAAGCCAAG CAGAAAACCT CAGAGTGGAT CGGGACACTA 
GTGGAGCAGC ACAGGGAGAC ACTGGACACA AAGTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The eutheria-specific miR-290 cluster modulates placental growth and maternal-fetal transport.
Development (Cambridge, England)144(20)3731-3743(2017 10)
Paikari A,D Belair C,Saw D,Blelloch R

Neuregulin 1 Regulates Proliferation of Leydig Cells to Support Spermatogenesis and Sexual Behavior in Adult Mice.
Endocrinology157(12)4899-4913(2016 Dec)
Umehara T,Kawashima I,Kawai T,Hoshino Y,Morohashi KI,Shima Y,Zeng W,Richards JS,Shimada M

A potential role for zinc transporter 7 in testosterone synthesis in mouse Leydig tumor cells.
International journal of molecular medicine37(6)1619-26(2016 Jun)
Chu Q,Chi ZH,Zhang X,Liang D,Wang X,Zhao Y,Zhang L,Zhang P

Steroidogenic factor 1 differentially regulates fetal and adult leydig cell development in male mice.
Biology of reproduction93(4)83(2015 Oct)
Karpova T,Ravichandiran K,Insisienmay L,Rice D,Agbor V,Heckert LL

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Hsd3b6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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オンラインフォーム

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