Hsd17b7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsd17b7
Entrez Gene ID	15490
Full Name	hydroxysteroid (17-beta) dehydrogenase 7
Synonyms	AI266814,ERG27
General protein information	Preferred Names hydroxysteroid (17-beta) dehydrogenase 7 Names 3-keto-steroid reductase 17-beta-HSD 7 17-beta-hydroxysteroid dehydrogenase 7 estradiol 17-beta-dehydrogenase 7
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011238758.2	XP_011237060.1	3-keto-steroid reductase isoform X1
XM_011238758.1	XP_011237060.1	3-keto-steroid reductase isoform X1
XM_006496667.2	XP_006496730.1	3-keto-steroid reductase isoform X3
XM_006496667.3	XP_006496730.1	3-keto-steroid reductase isoform X3
XM_017317108.1	XP_017172597.1	3-keto-steroid reductase isoform X2
XM_017317108.2	XP_017172597.1	3-keto-steroid reductase isoform X2
NM_010476.3	NP_034606.3	3-keto-steroid reductase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HSD17B7	XP_001174381.1
Bos taurus (cattle)	HSD17B7	NP_001076844.1
Gallus gallus (chicken)	HSD17B7	NP_001264435.1
Danio rerio (zebrafish)	hsd17b7	NP_001070796.1
Kluyveromyces lactis	KLLA0F19756g	XP_455967.1
Homo sapiens (human)	HSD17B7	NP_057455.1
Rattus norvegicus (Norway rat)	Hsd17b7	NP_058931.1
Danio rerio (zebrafish)	LOC100332819	XP_002662523.1
Xenopus tropicalis (tropical clawed frog)	hsd17b7	NP_001016209.1
	LOC720399	XP_002801925.1
Canis lupus familiaris (dog)	HSD17B7	XP_851698.1
Mus musculus (house mouse)	Hsd17b7	NP_034606.3
Eremothecium gossypii	AGOS_AGR068W	NP_986733.1
Schizosaccharomyces pombe (fission yeast)	erg27	NP_595440.1
Saccharomyces cerevisiae (baker's yeast)	ERG27	NP_013201.1

Gene Ontology
Bibliography

Related articles in PubMed

Altered cholesterol biosynthesis causes precocious neurogenesis in the developing mouse forebrain.
Driver AM, Kratz LE, Kelley RI, Stottmann RW
Neurobiology of disease9169-82(2016 07)

A compendium of expression patterns of cholesterol biosynthetic enzymes in the mouse embryo.
?i?ecio?lu M, Budak H, Geffers L, ?ankaya M, ?iftci M, Thaller C, Eichele G, K?frevio?lu ??, ?zdemir H
Journal of lipid research56(8)1551-9(2015 Aug)

Characterization of a mutation that results in independence of oxidosqualene cyclase (Erg7) activity from the downstream 3-ketoreductase (Erg27) in the yeast ergosterol biosynthetic pathway.
Layer JV, Barnes BM, Yamasaki Y, Barbuch R, Li L, Taramino S, Balliano G, Bard M
Biochimica et biophysica acta1831(2)361-9(2013 Feb)

Cholesterol metabolism is required for intracellular hedgehog signal transduction in vivo.
Stottmann RW, Turbe-Doan A, Tran P, Kratz LE, Moran JL, Kelley RI, Beier DR
PLoS genetics7(9)e1002224(2011 Sep)

Focusing forward genetics: a tripartite ENU screen for neurodevelopmental mutations in the mouse.
Stottmann RW, Moran JL, Turbe-Doan A, Driver E, Kelley M, Beier DR
Genetics188(3)615-24(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The ENU-induced rudolph mouse mutant has defects in both central nervous system and skeletal development, and carries a mutation of Hsd17b7. These developmental defects appear to be a consequence of abnormalities in Hedgehog signaling.
Title: Cholesterol metabolism is required for intracellular hedgehog signal transduction in vivo.

Hsd17b7 activity is essential for fetal de novo cholesterol synthesis and for neuroectodermal survival and cardiovascular differentiation in early mouse embryos
Title: Hydroxysteroid (17beta) dehydrogenase 7 activity is essential for fetal de novo cholesterol synthesis and for neuroectodermal survival and cardiovascular differentiation in early mouse embryos.

Data show that prolactin receptor-associated protein/17beta-hydroxysteroid dehydrogenase type 7 plays an important role in fetal survival and embryonic development in mice.
Title: Prolactin receptor-associated protein/17beta-hydroxysteroid dehydrogenase type 7 gene (Hsd17b7) plays a crucial role in embryonic development and fetal survival.

Results provide unequivocal evidence for a role of 17beta-hydroxysteroid dehydrogenase type-7 in cholesterol biosynthesis.
Title: Transcriptional regulation of human and murine 17beta-hydroxysteroid dehydrogenase type-7 confers its participation in cholesterol biosynthesis.

The identified proximal promoter regions of both human and murine HSD17B7 genes contain multiple transcription factor binding sites and show strong similarity to cholesterogenic genes.
Title: Analysis of the 5' flanking regions of human and murine HSD17B7: identification of a cholesterol dependent enhancer region.

The following Hsd17b7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsd17b7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06240	NM_010476.3 Latest version!	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu28630	XM_006496667.3 Latest version!	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu67969	XM_011238758.2 Latest version!	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu76983	XM_017317108.2 Latest version!	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu67969	XM_011238758.1	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu28630	XM_006496667.2	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu76983	XM_017317108.1	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsd17b7 ( NM_010476.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034606.3 Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), mRNA.

Price & Availability↑

CloneID	OMu06240
Clone ID Related Accession (Same CDS sequence)	NM_010476.3
Accession Version	NM_010476.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	3-keto-steroid reductase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY228199.1, AK050211.1 and BB767054.1. On Feb 11, 2006 this sequence version replaced NM_010476.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011464.1, AK050211.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCCTT CGCTTTTTTG TAAATGCGCT CACTGTGACA 
CCGTACAACG GAGCAGAGGC CCTGGTGTGG CTCTTCCACC AAAAACCGGA GTCTCTTAAT 
CCTCTGACCA AATACGCGAG CGCCACCTCG GGATTTGGGA CTAATTACGT CACGGGCCAA 
AAGATGGACA TAGATGAAGA CACTGCTGAA AAATTCTATG AGGTCTTACT GGAGCTGGAA 
AAGCGTGTCA GGACCACCGT TCAGAAATCG GATCACCCGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034606.3
CDS	74..1078
Translation	MRKVVLITGASSGIGLALCGRLLAEDDDLHLCLACRNLSKARAVRDTLLASHPSAEVSIVQMDVSSLQSVVRGAEEVKQKFQRLDYLYLNAGILPNPQFNLKAFFCGIFSRNVIHMFTTAEGILTQNDSVTADGLQEVFETNLFGHFILIRELEPLLCHADNPSQLIWTSSRNAKKANFSLEDIQHSKGPEPYSSSKYATDLLNVALNRNFNQKGLYSSVMCPGVVMTNMTYGILPPFIWTLLLPIMWLLRFFVNALTVTPYNGAEALVWLFHQKPESLNPLTKYASATSGFGTNYVTGQKMDIDEDTAEKFYEVLLELEKRVRTTVQKSDHPS

Target ORF information:

RefSeq Version	NM_010476.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010476.3

ORF Insert Sequence:

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCCTT CGCTTTTTTG TAAATGCGCT CACTGTGACA 
CCGTACAACG GAGCAGAGGC CCTGGTGTGG CTCTTCCACC AAAAACCGGA GTCTCTTAAT 
CCTCTGACCA AATACGCGAG CGCCACCTCG GGATTTGGGA CTAATTACGT CACGGGCCAA 
AAGATGGACA TAGATGAAGA CACTGCTGAA AAATTCTATG AGGTCTTACT GGAGCTGGAA 
AAGCGTGTCA GGACCACCGT TCAGAAATCG GATCACCCGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd17b7 ( XM_006496667.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496730.1 Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu28630
Clone ID Related Accession (Same CDS sequence)	XM_006496667.2 , XM_006496667.3
Accession Version	XM_006496667.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	3-keto-steroid reductase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006496667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCGTG TGGCTCTTCC ACCAAAAACC GGAGTCTCTT 
AATCCTCTGA CCAAATACGC GAGCGCCACC TCGGGATTTG GGACTAATTA CGTCACGGGC 
CAAAAGATGG ACATAGATGA AGACACTGCT GAAAAATTCT ATGAGGTCTT ACTGGAGCTG 
GAAAAGCGTG TCAGGACCAC CGTTCAGAAA TCGGATCACC CGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496730.1
CDS	105..1052
Translation	MRKVVLITGASSGIGLALCGRLLAEDDDLHLCLACRNLSKARAVRDTLLASHPSAEVSIVQMDVSSLQSVVRGAEEVKQKFQRLDYLYLNAGILPNPQFNLKAFFCGIFSRNVIHMFTTAEGILTQNDSVTADGLQEVFETNLFGHFILIRELEPLLCHADNPSQLIWTSSRNAKKANFSLEDIQHSKGPEPYSSSKYATDLLNVALNRNFNQKGLYSSVMCPGVVMTNMTYGILPPFIWTLLLPIMWLVWLFHQKPESLNPLTKYASATSGFGTNYVTGQKMDIDEDTAEKFYEVLLELEKRVRTTVQKSDHPS

Target ORF information:

RefSeq Version	XM_006496667.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496667.2

ORF Insert Sequence:

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCGTG TGGCTCTTCC ACCAAAAACC GGAGTCTCTT 
AATCCTCTGA CCAAATACGC GAGCGCCACC TCGGGATTTG GGACTAATTA CGTCACGGGC 
CAAAAGATGG ACATAGATGA AGACACTGCT GAAAAATTCT ATGAGGTCTT ACTGGAGCTG 
GAAAAGCGTG TCAGGACCAC CGTTCAGAAA TCGGATCACC CGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd17b7 ( XM_011238758.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237060.1 Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67969
Clone ID Related Accession (Same CDS sequence)	XM_011238758.2 , XM_011238758.1
Accession Version	XM_011238758.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	3-keto-steroid reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCCTT CGCTTTTTTG TAAATGCGCT CACTGTGACA 
CCGTACAACG GAGCAGAGGC CCTGGTGTGG CTCTTCCACC AAAAACCGGA GTCTCTTAAT 
CCTCTGACCA AATACGCGAG CGCCACCTCG GGATTTGGGA CTAATTACGT CACGGGCCAA 
AAGGTACATA ATCCGTTGAT GTTGTCAAGT GTGATGTTCA CAAACCCATC TGCTTGGCTT 
CTGCATCCGA GTCCTCATGC TGGCTTTATC ACGGTCCATT TCATTACTCT CAGCTTTCTC 
CTGTCTCAGA GTGCCGGGCA TCTTCTTTCT GCTAAGCCTC TCTCACCTTT GTTCTTGTTG 
ATGTCTGTCA CCCATCAGAA CTCAGTTTCT TCCCAGCCTT CTCTGTCTCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237060.1
CDS	107..1240
Translation	MRKVVLITGASSGIGLALCGRLLAEDDDLHLCLACRNLSKARAVRDTLLASHPSAEVSIVQMDVSSLQSVVRGAEEVKQKFQRLDYLYLNAGILPNPQFNLKAFFCGIFSRNVIHMFTTAEGILTQNDSVTADGLQEVFETNLFGHFILIRELEPLLCHADNPSQLIWTSSRNAKKANFSLEDIQHSKGPEPYSSSKYATDLLNVALNRNFNQKGLYSSVMCPGVVMTNMTYGILPPFIWTLLLPIMWLLRFFVNALTVTPYNGAEALVWLFHQKPESLNPLTKYASATSGFGTNYVTGQKVHNPLMLSSVMFTNPSAWLLHPSPHAGFITVHFITLSFLLSQSAGHLLSAKPLSPLFLLMSVTHQNSVSSQPSLSL

Target ORF information:

RefSeq Version	XM_011238758.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238758.1

ORF Insert Sequence:

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCCTT CGCTTTTTTG TAAATGCGCT CACTGTGACA 
CCGTACAACG GAGCAGAGGC CCTGGTGTGG CTCTTCCACC AAAAACCGGA GTCTCTTAAT 
CCTCTGACCA AATACGCGAG CGCCACCTCG GGATTTGGGA CTAATTACGT CACGGGCCAA 
AAGGTACATA ATCCGTTGAT GTTGTCAAGT GTGATGTTCA CAAACCCATC TGCTTGGCTT 
CTGCATCCGA GTCCTCATGC TGGCTTTATC ACGGTCCATT TCATTACTCT CAGCTTTCTC 
CTGTCTCAGA GTGCCGGGCA TCTTCTTTCT GCTAAGCCTC TCTCACCTTT GTTCTTGTTG 
ATGTCTGTCA CCCATCAGAA CTCAGTTTCT TCCCAGCCTT CTCTGTCTCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd17b7 ( XM_006496667.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496730.1 Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X3, mRNA.

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CloneID	OMu28630
Clone ID Related Accession (Same CDS sequence)	XM_006496667.2 , XM_006496667.3
Accession Version	XM_006496667.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	3-keto-steroid reductase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCGTG TGGCTCTTCC ACCAAAAACC GGAGTCTCTT 
AATCCTCTGA CCAAATACGC GAGCGCCACC TCGGGATTTG GGACTAATTA CGTCACGGGC 
CAAAAGATGG ACATAGATGA AGACACTGCT GAAAAATTCT ATGAGGTCTT ACTGGAGCTG 
GAAAAGCGTG TCAGGACCAC CGTTCAGAAA TCGGATCACC CGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496730.1
CDS	109..1056
Translation	MRKVVLITGASSGIGLALCGRLLAEDDDLHLCLACRNLSKARAVRDTLLASHPSAEVSIVQMDVSSLQSVVRGAEEVKQKFQRLDYLYLNAGILPNPQFNLKAFFCGIFSRNVIHMFTTAEGILTQNDSVTADGLQEVFETNLFGHFILIRELEPLLCHADNPSQLIWTSSRNAKKANFSLEDIQHSKGPEPYSSSKYATDLLNVALNRNFNQKGLYSSVMCPGVVMTNMTYGILPPFIWTLLLPIMWLVWLFHQKPESLNPLTKYASATSGFGTNYVTGQKMDIDEDTAEKFYEVLLELEKRVRTTVQKSDHPS

Target ORF information:

RefSeq Version	XM_006496667.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496667.3

ORF Insert Sequence:

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCGTG TGGCTCTTCC ACCAAAAACC GGAGTCTCTT 
AATCCTCTGA CCAAATACGC GAGCGCCACC TCGGGATTTG GGACTAATTA CGTCACGGGC 
CAAAAGATGG ACATAGATGA AGACACTGCT GAAAAATTCT ATGAGGTCTT ACTGGAGCTG 
GAAAAGCGTG TCAGGACCAC CGTTCAGAAA TCGGATCACC CGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd17b7 ( XM_011238758.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237060.1 Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67969
Clone ID Related Accession (Same CDS sequence)	XM_011238758.2 , XM_011238758.1
Accession Version	XM_011238758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	3-keto-steroid reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCCTT CGCTTTTTTG TAAATGCGCT CACTGTGACA 
CCGTACAACG GAGCAGAGGC CCTGGTGTGG CTCTTCCACC AAAAACCGGA GTCTCTTAAT 
CCTCTGACCA AATACGCGAG CGCCACCTCG GGATTTGGGA CTAATTACGT CACGGGCCAA 
AAGGTACATA ATCCGTTGAT GTTGTCAAGT GTGATGTTCA CAAACCCATC TGCTTGGCTT 
CTGCATCCGA GTCCTCATGC TGGCTTTATC ACGGTCCATT TCATTACTCT CAGCTTTCTC 
CTGTCTCAGA GTGCCGGGCA TCTTCTTTCT GCTAAGCCTC TCTCACCTTT GTTCTTGTTG 
ATGTCTGTCA CCCATCAGAA CTCAGTTTCT TCCCAGCCTT CTCTGTCTCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237060.1
CDS	110..1243
Translation	MRKVVLITGASSGIGLALCGRLLAEDDDLHLCLACRNLSKARAVRDTLLASHPSAEVSIVQMDVSSLQSVVRGAEEVKQKFQRLDYLYLNAGILPNPQFNLKAFFCGIFSRNVIHMFTTAEGILTQNDSVTADGLQEVFETNLFGHFILIRELEPLLCHADNPSQLIWTSSRNAKKANFSLEDIQHSKGPEPYSSSKYATDLLNVALNRNFNQKGLYSSVMCPGVVMTNMTYGILPPFIWTLLLPIMWLLRFFVNALTVTPYNGAEALVWLFHQKPESLNPLTKYASATSGFGTNYVTGQKVHNPLMLSSVMFTNPSAWLLHPSPHAGFITVHFITLSFLLSQSAGHLLSAKPLSPLFLLMSVTHQNSVSSQPSLSL

Target ORF information:

RefSeq Version	XM_011238758.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238758.2

ORF Insert Sequence:

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCCTT CGCTTTTTTG TAAATGCGCT CACTGTGACA 
CCGTACAACG GAGCAGAGGC CCTGGTGTGG CTCTTCCACC AAAAACCGGA GTCTCTTAAT 
CCTCTGACCA AATACGCGAG CGCCACCTCG GGATTTGGGA CTAATTACGT CACGGGCCAA 
AAGGTACATA ATCCGTTGAT GTTGTCAAGT GTGATGTTCA CAAACCCATC TGCTTGGCTT 
CTGCATCCGA GTCCTCATGC TGGCTTTATC ACGGTCCATT TCATTACTCT CAGCTTTCTC 
CTGTCTCAGA GTGCCGGGCA TCTTCTTTCT GCTAAGCCTC TCTCACCTTT GTTCTTGTTG 
ATGTCTGTCA CCCATCAGAA CTCAGTTTCT TCCCAGCCTT CTCTGTCTCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd17b7 ( XM_017317108.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172597.1 Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu76983
Clone ID Related Accession (Same CDS sequence)	XM_017317108.1 , XM_017317108.2
Accession Version	XM_017317108.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	3-keto-steroid reductase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCGTG TGGCTCTTCC ACCAAAAACC GGAGTCTCTT 
AATCCTCTGA CCAAATACGC GAGCGCCACC TCGGGATTTG GGACTAATTA CGTCACGGGC 
CAAAAGGTAC ATAATCCGTT GATGTTGTCA AGTGTGATGT TCACAAACCC ATCTGCTTGG 
CTTCTGCATC CGAGTCCTCA TGCTGGCTTT ATCACGGTCC ATTTCATTAC TCTCAGCTTT 
CTCCTGTCTC AGAGTGCCGG GCATCTTCTT TCTGCTAAGC CTCTCTCACC TTTGTTCTTG 
TTGATGTCTG TCACCCATCA GAACTCAGTT TCTTCCCAGC CTTCTCTGTC TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172597.1
CDS	109..1185
Translation	MRKVVLITGASSGIGLALCGRLLAEDDDLHLCLACRNLSKARAVRDTLLASHPSAEVSIVQMDVSSLQSVVRGAEEVKQKFQRLDYLYLNAGILPNPQFNLKAFFCGIFSRNVIHMFTTAEGILTQNDSVTADGLQEVFETNLFGHFILIRELEPLLCHADNPSQLIWTSSRNAKKANFSLEDIQHSKGPEPYSSSKYATDLLNVALNRNFNQKGLYSSVMCPGVVMTNMTYGILPPFIWTLLLPIMWLVWLFHQKPESLNPLTKYASATSGFGTNYVTGQKVHNPLMLSSVMFTNPSAWLLHPSPHAGFITVHFITLSFLLSQSAGHLLSAKPLSPLFLLMSVTHQNSVSSQPSLSL

Target ORF information:

RefSeq Version	XM_017317108.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317108.1

ORF Insert Sequence:

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCGTG TGGCTCTTCC ACCAAAAACC GGAGTCTCTT 
AATCCTCTGA CCAAATACGC GAGCGCCACC TCGGGATTTG GGACTAATTA CGTCACGGGC 
CAAAAGGTAC ATAATCCGTT GATGTTGTCA AGTGTGATGT TCACAAACCC ATCTGCTTGG 
CTTCTGCATC CGAGTCCTCA TGCTGGCTTT ATCACGGTCC ATTTCATTAC TCTCAGCTTT 
CTCCTGTCTC AGAGTGCCGG GCATCTTCTT TCTGCTAAGC CTCTCTCACC TTTGTTCTTG 
TTGATGTCTG TCACCCATCA GAACTCAGTT TCTTCCCAGC CTTCTCTGTC TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd17b7 ( XM_017317108.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172597.1 Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu76983
Clone ID Related Accession (Same CDS sequence)	XM_017317108.1 , XM_017317108.2
Accession Version	XM_017317108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	3-keto-steroid reductase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCGTG TGGCTCTTCC ACCAAAAACC GGAGTCTCTT 
AATCCTCTGA CCAAATACGC GAGCGCCACC TCGGGATTTG GGACTAATTA CGTCACGGGC 
CAAAAGGTAC ATAATCCGTT GATGTTGTCA AGTGTGATGT TCACAAACCC ATCTGCTTGG 
CTTCTGCATC CGAGTCCTCA TGCTGGCTTT ATCACGGTCC ATTTCATTAC TCTCAGCTTT 
CTCCTGTCTC AGAGTGCCGG GCATCTTCTT TCTGCTAAGC CTCTCTCACC TTTGTTCTTG 
TTGATGTCTG TCACCCATCA GAACTCAGTT TCTTCCCAGC CTTCTCTGTC TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172597.1
CDS	111..1187
Translation	MRKVVLITGASSGIGLALCGRLLAEDDDLHLCLACRNLSKARAVRDTLLASHPSAEVSIVQMDVSSLQSVVRGAEEVKQKFQRLDYLYLNAGILPNPQFNLKAFFCGIFSRNVIHMFTTAEGILTQNDSVTADGLQEVFETNLFGHFILIRELEPLLCHADNPSQLIWTSSRNAKKANFSLEDIQHSKGPEPYSSSKYATDLLNVALNRNFNQKGLYSSVMCPGVVMTNMTYGILPPFIWTLLLPIMWLVWLFHQKPESLNPLTKYASATSGFGTNYVTGQKVHNPLMLSSVMFTNPSAWLLHPSPHAGFITVHFITLSFLLSQSAGHLLSAKPLSPLFLLMSVTHQNSVSSQPSLSL

Target ORF information:

RefSeq Version	XM_017317108.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxysteroid (17-beta) dehydrogenase 7 (Hsd17b7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317108.2

ORF Insert Sequence:

ATGCGGAAGG TGGTTTTGAT CACCGGGGCG AGCAGTGGCA TTGGGCTAGC CCTTTGCGGT 
CGACTGCTGG CAGAAGACGA TGACCTCCAC CTGTGTTTGG CGTGTAGGAA CCTGAGCAAA 
GCAAGAGCTG TTCGAGATAC CCTGCTGGCC TCTCACCCCT CCGCCGAAGT CAGCATCGTG 
CAGATGGATG TCAGCAGCCT GCAGTCGGTG GTCCGGGGTG CAGAGGAAGT CAAGCAAAAG 
TTTCAAAGAT TAGACTACTT ATATCTGAAT GCTGGAATCC TGCCTAATCC ACAATTCAAC 
CTCAAGGCAT TTTTCTGCGG CATCTTTTCA AGAAATGTGA TTCATATGTT CACCACAGCG 
GAAGGAATTT TGACCCAGAA TGACTCGGTC ACTGCCGACG GGTTGCAGGA GGTGTTTGAA 
ACCAATCTCT TTGGCCACTT TATTCTGATT CGGGAACTGG AACCACTTCT CTGCCATGCG 
GACAACCCCT CTCAGCTCAT CTGGACGTCC TCTCGCAATG CAAAGAAGGC TAACTTCAGC 
CTGGAGGACA TCCAGCACTC CAAAGGCCCG GAACCCTACA GCTCTTCCAA ATATGCTACC 
GACCTCCTGA ATGTGGCTTT GAACAGGAAT TTCAACCAGA AGGGTCTGTA TTCCAGTGTG 
ATGTGCCCAG GCGTCGTGAT GACCAATATG ACGTATGGAA TTTTGCCTCC CTTTATCTGG 
ACGTTGCTCC TACCCATAAT GTGGCTCGTG TGGCTCTTCC ACCAAAAACC GGAGTCTCTT 
AATCCTCTGA CCAAATACGC GAGCGCCACC TCGGGATTTG GGACTAATTA CGTCACGGGC 
CAAAAGGTAC ATAATCCGTT GATGTTGTCA AGTGTGATGT TCACAAACCC ATCTGCTTGG 
CTTCTGCATC CGAGTCCTCA TGCTGGCTTT ATCACGGTCC ATTTCATTAC TCTCAGCTTT 
CTCCTGTCTC AGAGTGCCGG GCATCTTCTT TCTGCTAAGC CTCTCTCACC TTTGTTCTTG 
TTGATGTCTG TCACCCATCA GAACTCAGTT TCTTCCCAGC CTTCTCTGTC TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered cholesterol biosynthesis causes precocious neurogenesis in the developing mouse forebrain.
Neurobiology of disease9169-82(2016 07)
Driver AM,Kratz LE,Kelley RI,Stottmann RW

A compendium of expression patterns of cholesterol biosynthetic enzymes in the mouse embryo.
Journal of lipid research56(8)1551-9(2015 Aug)
?i?ecio?lu M,Budak H,Geffers L,?ankaya M,?iftci M,Thaller C,Eichele G,K?frevio?lu ??,?zdemir H

Characterization of a mutation that results in independence of oxidosqualene cyclase (Erg7) activity from the downstream 3-ketoreductase (Erg27) in the yeast ergosterol biosynthetic pathway.
Biochimica et biophysica acta1831(2)361-9(2013 Feb)
Layer JV,Barnes BM,Yamasaki Y,Barbuch R,Li L,Taramino S,Balliano G,Bard M

Cholesterol metabolism is required for intracellular hedgehog signal transduction in vivo.
PLoS genetics7(9)e1002224(2011 Sep)
Stottmann RW,Turbe-Doan A,Tran P,Kratz LE,Moran JL,Kelley RI,Beier DR

Focusing forward genetics: a tripartite ENU screen for neurodevelopmental mutations in the mouse.
Genetics188(3)615-24(2011 Jul)
Stottmann RW,Moran JL,Turbe-Doan A,Driver E,Kelley M,Beier DR

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Hsd17b7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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