Hspa8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspa8
Entrez Gene ID	15481
Full Name	heat shock protein 8
Synonyms	2410008N15Rik,Hsc70,Hsc71,Hsc73,Hsp73,Hspa10
General protein information	Preferred Names heat shock protein 8 Names heat shock cognate 71 kDa protein heat shock 70 kDa protein 8 heat shock 70kD protein 8 heat shock cognate hsc73 heat shock protein cognate 70
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 A5.1\|9 21.55 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_031165.5	NP_112442.2	heat shock cognate 71 kDa protein isoform 1
NM_001364480.1	NP_001351409.1	heat shock cognate 71 kDa protein isoform 2
NM_031165.4	NP_112442.2	heat shock cognate 71 kDa protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	HSPA8	NP_006588.1
	HSPA8	XP_002799868.1
Canis lupus familiaris (dog)	HSPA8	XP_005619707.1
Bos taurus (cattle)	HSPA8	NP_776770.2
Rattus norvegicus (Norway rat)	Hspa8	NP_077327.1
Gallus gallus (chicken)	HSPA8	NP_990334.1
Caenorhabditis elegans (roundworm)	hsp-1	NP_503068.1
Mus musculus (house mouse)	Hspa8	NP_112442.2
Danio rerio (zebrafish)	hspa8	NP_001103873.1
Xenopus tropicalis (tropical clawed frog)	hspa8	XP_004916187.1
Pan troglodytes (chimpanzee)	HSPA8	XP_003313441.2
Drosophila melanogaster (fruit fly)	Hsc70-1	NP_524063.1
Arabidopsis thaliana (thale cress)	AT3G09440	NP_001189847.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004944	XP_315042.3

Gene Ontology
Bibliography

Related articles in PubMed

Post-transcriptional Inhibition of Hsc70-4/HSPA8 Expression Leads to Synaptic Vesicle Cycling Defects in Multiple Models of ALS.
Coyne AN, Lorenzini I, Chou CC, Torvund M, Rogers RS, Starr A, Zaepfel BL, Levy J, Johannesmeyer J, Schwartz JC, Nishimune H, Zinsmaier K, Rossoll W, Sattler R, Zarnescu DC
Cell reports21(1)110-125(2017 Oct)

Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin.
Ganguly A, Han X, Das U, Wang L, Loi J, Sun J, Gitler D, Caillol G, Leterrier C, Yates JR 3rd, Roy S
The Journal of cell biology216(7)2059-2074(2017 Jul)

Salmonella Co-opts Host Cell Chaperone-mediated Autophagy for Intracellular Growth.
Singh V, Finke-Isami J, Hopper-Chidlaw AC, Schwerk P, Thompson A, Tedin K
The Journal of biological chemistry292(5)1847-1864(2017 02)

Functional analysis of HSPA1A and HSPA8 in parturition.
Geng J, Li H, Huang C, Chai J, Zheng R, Li F, Jiang S
Biochemical and biophysical research communications483(1)371-379(2017 01)

Subcellular distribution of non-muscle myosin IIb is controlled by FILIP through Hsc70.
Yagi H, Takabayashi T, Xie MJ, Kuroda K, Sato M
PloS one12(2)e0172257(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Synapsin is part of a multiprotein complex enriched in chaperones/cochaperones including Hsc70. Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin.
Title: Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin.

intracellular Salmonella recruit the host proteins LAMP-2A and Hsc73, key components of the host protein turnover pathway known as chaperone-mediated autophagy involved in transport of cytosolic proteins to the lysosome for degradation.
Title: Salmonella Co-opts Host Cell Chaperone-mediated Autophagy for Intracellular Growth.

HSPA1A and HSPA8 have roles in parturition through stimulating immune inflammatory and estrogen response
Title: Functional analysis of HSPA1A and HSPA8 in parturition.

Hsc70 interacts with FILIP to mediate its effects on non-muscle myosin IIb and to regulate spine morphology
Title: Subcellular distribution of non-muscle myosin IIb is controlled by FILIP through Hsc70.

the association of MNSFbeta with HSPA8 may promote RANKL-induced osteoclastogenesis.
Title: Ubiquitin-like protein MNSF? noncovalently binds to molecular chaperone HSPA8 and regulates osteoclastogenesis.

The following Hspa8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspa8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15198	NM_031165.5 Latest version!	Mus musculus heat shock protein 8 (Hspa8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu83985	NM_001364480.1 Latest version!	Mus musculus heat shock protein 8 (Hspa8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu15198	NM_031165.4	Mus musculus heat shock protein 8 (Hspa8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspa8 ( NM_031165.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_112442.2 Mus musculus heat shock protein 8 (Hspa8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15198
Clone ID Related Accession (Same CDS sequence)	NM_031165.5 , NM_031165.4
Accession Version	NM_031165.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Mus musculus(house mouse)
Product	heat shock cognate 71 kDa protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK151775.1 and CD774246.1. On Jun 19, 2018 this sequence version replaced NM_031165.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.212272.1, BC085486.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTAAGG GACCTGCAGT TGGCATTGAT CTCGGCACCA CCTACTCCTG TGTGGGTGTC 
TTCCAGCATG GAAAGGTGGA AATTATTGCC AATGACCAGG GTAACCGCAC CACGCCAAGC 
TATGTTGCTT TCACGGACAC AGAGAGATTA ATTGGGGATG CGGCCAAGAA TCAGGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TCGGGCGTAG GTTTGATGAT 
GCTGTTGTTC AGTCTGATAT GAAGCACTGG CCCTTCATGG TGGTGAATGA TGCAGGCAGG 
CCCAAGGTCC AAGTGGAATA CAAAGGGGAG ACAAAAAGTT TCTACCCAGA GGAAGTGTCC 
TCCATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCGT ACCTCGGAAA GACCGTTACC 
AACGCTGTGG TCACAGTGCC CGCTTACTTC AATGACTCTC AGCGACAGGC AACAAAAGAT 
GCTGGAACTA TTGCTGGCCT CAATGTACTT CGAATCATCA ATGAACCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGATAA GAAGGTCGGA GCTGAAAGGA ATGTGCTCAT TTTTGACTTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATTTT TGAGGTCAAA 
TCAACAGCTG GAGACACCCA CTTAGGTGGA GAAGATTTTG ACAACCGAAT GGTCAATCAT 
TTCATTGCTG AGTTCAAGCG AAAGCACAAG AAAGACATCA GTGAGAACAA GAGAGCTGTC 
CGCCGTCTCC GCACGGCCTG CGAGCGGGCC AAGCGCACCC TCTCCTCCAG CACCCAGGCC 
AGTATTGAGA TTGATTCTCT CTATGAGGGA ATTGACTTCT ATACCTCCAT TACCCGGGCT 
CGATTTGAGG AGTTGAATGC TGACCTGTTC CGTGGCACAC TGGACCCTGT AGAGAAGGCC 
CTTCGAGATG CCAAGCTGGA CAAGTCACAG ATCCATGATA TTGTCTTGGT GGGTGGTTCT 
ACCAGAATCC CCAAGATTCA GAAACTTCTG CAAGACTTCT TCAATGGAAA AGAGCTGAAC 
AAGAGCATTA ACCCCGATGA AGCTGTTGCC TATGGTGCAG CTGTCCAGGC AGCCATTCTA 
TCTGGAGACA AGTCTGAGAA CGTTCAGGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG CGGAGTCATG ACTGTCCTCA TCAAGCGCAA TACCACCATC 
CCCACCAAGC AGACACAGAC TTTCACCACC TACTCTGACA ACCAGCCTGG TGTACTCATT 
CAGGTGTATG AAGGTGAAAG GGCCATGACC AAGGACAACA ACCTGCTTGG AAAGTTCGAG 
CTCACAGGCA TCCCTCCAGC ACCCCGTGGG GTCCCTCAGA TTGAGGTTAC TTTTGACATC 
GATGCCAATG GCATCCTCAA TGTTTCTGCT GTAGATAAGA GCACAGGAAA GGAGAACAAG 
ATCACCATCA CCAATGACAA GGGCCGCTTG AGTAAGGAAG ATATTGAGCG CATGGTCCAA 
GAAGCTGAGA AGTACAAGGC TGAGGATGAG AAGCAGAGAG ATAAGGTTTC CTCCAAGAAC 
TCACTGGAGT CCTATGCCTT CAACATGAAA GCAACTGTGG AAGATGAGAA ACTTCAAGGC 
AAGATCAATG ATGAGGACAA ACAGAAGATT CTTGACAAGT GCAATGAAAT CATCAGCTGG 
CTGGATAAGA ACCAGACTGC AGAGAAGGAA GAATTTGAGC ATCAGCAGAA AGAACTGGAG 
AAAGTCTGCA ACCCTATCAT TACCAAGCTG TACCAGAGTG CAGGTGGCAT GCCTGGGGGA 
ATGCCTGGTG GCTTCCCAGG TGGAGGAGCT CCCCCATCTG GTGGTGCTTC TTCAGGCCCC 
ACCATTGAAG AGGTGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112442.2
CDS	79..2019
Translation	MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIISWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

Target ORF information:

RefSeq Version	NM_031165.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 8 (Hspa8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031165.5

ORF Insert Sequence:

ATGTCTAAGG GACCTGCAGT TGGCATTGAT CTCGGCACCA CCTACTCCTG TGTGGGTGTC 
TTCCAGCATG GAAAGGTGGA AATTATTGCC AATGACCAGG GTAACCGCAC CACGCCAAGC 
TATGTTGCTT TCACGGACAC AGAGAGATTA ATTGGGGATG CGGCCAAGAA TCAGGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TCGGGCGTAG GTTTGATGAT 
GCTGTTGTTC AGTCTGATAT GAAGCACTGG CCCTTCATGG TGGTGAATGA TGCAGGCAGG 
CCCAAGGTCC AAGTGGAATA CAAAGGGGAG ACAAAAAGTT TCTACCCAGA GGAAGTGTCC 
TCCATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCGT ACCTCGGAAA GACCGTTACC 
AACGCTGTGG TCACAGTGCC CGCTTACTTC AATGACTCTC AGCGACAGGC AACAAAAGAT 
GCTGGAACTA TTGCTGGCCT CAATGTACTT CGAATCATCA ATGAACCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGATAA GAAGGTCGGA GCTGAAAGGA ATGTGCTCAT TTTTGACTTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATTTT TGAGGTCAAA 
TCAACAGCTG GAGACACCCA CTTAGGTGGA GAAGATTTTG ACAACCGAAT GGTCAATCAT 
TTCATTGCTG AGTTCAAGCG AAAGCACAAG AAAGACATCA GTGAGAACAA GAGAGCTGTC 
CGCCGTCTCC GCACGGCCTG CGAGCGGGCC AAGCGCACCC TCTCCTCCAG CACCCAGGCC 
AGTATTGAGA TTGATTCTCT CTATGAGGGA ATTGACTTCT ATACCTCCAT TACCCGGGCT 
CGATTTGAGG AGTTGAATGC TGACCTGTTC CGTGGCACAC TGGACCCTGT AGAGAAGGCC 
CTTCGAGATG CCAAGCTGGA CAAGTCACAG ATCCATGATA TTGTCTTGGT GGGTGGTTCT 
ACCAGAATCC CCAAGATTCA GAAACTTCTG CAAGACTTCT TCAATGGAAA AGAGCTGAAC 
AAGAGCATTA ACCCCGATGA AGCTGTTGCC TATGGTGCAG CTGTCCAGGC AGCCATTCTA 
TCTGGAGACA AGTCTGAGAA CGTTCAGGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG CGGAGTCATG ACTGTCCTCA TCAAGCGCAA TACCACCATC 
CCCACCAAGC AGACACAGAC TTTCACCACC TACTCTGACA ACCAGCCTGG TGTACTCATT 
CAGGTGTATG AAGGTGAAAG GGCCATGACC AAGGACAACA ACCTGCTTGG AAAGTTCGAG 
CTCACAGGCA TCCCTCCAGC ACCCCGTGGG GTCCCTCAGA TTGAGGTTAC TTTTGACATC 
GATGCCAATG GCATCCTCAA TGTTTCTGCT GTAGATAAGA GCACAGGAAA GGAGAACAAG 
ATCACCATCA CCAATGACAA GGGCCGCTTG AGTAAGGAAG ATATTGAGCG CATGGTCCAA 
GAAGCTGAGA AGTACAAGGC TGAGGATGAG AAGCAGAGAG ATAAGGTTTC CTCCAAGAAC 
TCACTGGAGT CCTATGCCTT CAACATGAAA GCAACTGTGG AAGATGAGAA ACTTCAAGGC 
AAGATCAATG ATGAGGACAA ACAGAAGATT CTTGACAAGT GCAATGAAAT CATCAGCTGG 
CTGGATAAGA ACCAGACTGC AGAGAAGGAA GAATTTGAGC ATCAGCAGAA AGAACTGGAG 
AAAGTCTGCA ACCCTATCAT TACCAAGCTG TACCAGAGTG CAGGTGGCAT GCCTGGGGGA 
ATGCCTGGTG GCTTCCCAGG TGGAGGAGCT CCCCCATCTG GTGGTGCTTC TTCAGGCCCC 
ACCATTGAAG AGGTGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa8 ( NM_031165.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_112442.2 Mus musculus heat shock protein 8 (Hspa8), mRNA.

Price & Availability↑

CloneID	OMu15198
Clone ID Related Accession (Same CDS sequence)	NM_031165.5 , NM_031165.4
Accession Version	NM_031165.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	heat shock cognate 71 kDa protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK151775.1. On or before Jul 21, 2011 this sequence version replaced NR_015570.1, NM_031165.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK151775.1, AK153032.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCTAAGG GACCTGCAGT TGGCATTGAT CTCGGCACCA CCTACTCCTG TGTGGGTGTC 
TTCCAGCATG GAAAGGTGGA AATTATTGCC AATGACCAGG GTAACCGCAC CACGCCAAGC 
TATGTTGCTT TCACGGACAC AGAGAGATTA ATTGGGGATG CGGCCAAGAA TCAGGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TCGGGCGTAG GTTTGATGAT 
GCTGTTGTTC AGTCTGATAT GAAGCACTGG CCCTTCATGG TGGTGAATGA TGCAGGCAGG 
CCCAAGGTCC AAGTGGAATA CAAAGGGGAG ACAAAAAGTT TCTACCCAGA GGAAGTGTCC 
TCCATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCGT ACCTCGGAAA GACCGTTACC 
AACGCTGTGG TCACAGTGCC CGCTTACTTC AATGACTCTC AGCGACAGGC AACAAAAGAT 
GCTGGAACTA TTGCTGGCCT CAATGTACTT CGAATCATCA ATGAACCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGATAA GAAGGTCGGA GCTGAAAGGA ATGTGCTCAT TTTTGACTTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATTTT TGAGGTCAAA 
TCAACAGCTG GAGACACCCA CTTAGGTGGA GAAGATTTTG ACAACCGAAT GGTCAATCAT 
TTCATTGCTG AGTTCAAGCG AAAGCACAAG AAAGACATCA GTGAGAACAA GAGAGCTGTC 
CGCCGTCTCC GCACGGCCTG CGAGCGGGCC AAGCGCACCC TCTCCTCCAG CACCCAGGCC 
AGTATTGAGA TTGATTCTCT CTATGAGGGA ATTGACTTCT ATACCTCCAT TACCCGGGCT 
CGATTTGAGG AGTTGAATGC TGACCTGTTC CGTGGCACAC TGGACCCTGT AGAGAAGGCC 
CTTCGAGATG CCAAGCTGGA CAAGTCACAG ATCCATGATA TTGTCTTGGT GGGTGGTTCT 
ACCAGAATCC CCAAGATTCA GAAACTTCTG CAAGACTTCT TCAATGGAAA AGAGCTGAAC 
AAGAGCATTA ACCCCGATGA AGCTGTTGCC TATGGTGCAG CTGTCCAGGC AGCCATTCTA 
TCTGGAGACA AGTCTGAGAA CGTTCAGGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG CGGAGTCATG ACTGTCCTCA TCAAGCGCAA TACCACCATC 
CCCACCAAGC AGACACAGAC TTTCACCACC TACTCTGACA ACCAGCCTGG TGTACTCATT 
CAGGTGTATG AAGGTGAAAG GGCCATGACC AAGGACAACA ACCTGCTTGG AAAGTTCGAG 
CTCACAGGCA TCCCTCCAGC ACCCCGTGGG GTCCCTCAGA TTGAGGTTAC TTTTGACATC 
GATGCCAATG GCATCCTCAA TGTTTCTGCT GTAGATAAGA GCACAGGAAA GGAGAACAAG 
ATCACCATCA CCAATGACAA GGGCCGCTTG AGTAAGGAAG ATATTGAGCG CATGGTCCAA 
GAAGCTGAGA AGTACAAGGC TGAGGATGAG AAGCAGAGAG ATAAGGTTTC CTCCAAGAAC 
TCACTGGAGT CCTATGCCTT CAACATGAAA GCAACTGTGG AAGATGAGAA ACTTCAAGGC 
AAGATCAATG ATGAGGACAA ACAGAAGATT CTTGACAAGT GCAATGAAAT CATCAGCTGG 
CTGGATAAGA ACCAGACTGC AGAGAAGGAA GAATTTGAGC ATCAGCAGAA AGAACTGGAG 
AAAGTCTGCA ACCCTATCAT TACCAAGCTG TACCAGAGTG CAGGTGGCAT GCCTGGGGGA 
ATGCCTGGTG GCTTCCCAGG TGGAGGAGCT CCCCCATCTG GTGGTGCTTC TTCAGGCCCC 
ACCATTGAAG AGGTGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112442.2
CDS	82..2022
Translation	MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIISWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

Target ORF information:

RefSeq Version	NM_031165.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 8 (Hspa8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031165.4

ORF Insert Sequence:

ATGTCTAAGG GACCTGCAGT TGGCATTGAT CTCGGCACCA CCTACTCCTG TGTGGGTGTC 
TTCCAGCATG GAAAGGTGGA AATTATTGCC AATGACCAGG GTAACCGCAC CACGCCAAGC 
TATGTTGCTT TCACGGACAC AGAGAGATTA ATTGGGGATG CGGCCAAGAA TCAGGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TCGGGCGTAG GTTTGATGAT 
GCTGTTGTTC AGTCTGATAT GAAGCACTGG CCCTTCATGG TGGTGAATGA TGCAGGCAGG 
CCCAAGGTCC AAGTGGAATA CAAAGGGGAG ACAAAAAGTT TCTACCCAGA GGAAGTGTCC 
TCCATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCGT ACCTCGGAAA GACCGTTACC 
AACGCTGTGG TCACAGTGCC CGCTTACTTC AATGACTCTC AGCGACAGGC AACAAAAGAT 
GCTGGAACTA TTGCTGGCCT CAATGTACTT CGAATCATCA ATGAACCAAC TGCTGCTGCT 
ATTGCTTACG GCTTAGATAA GAAGGTCGGA GCTGAAAGGA ATGTGCTCAT TTTTGACTTG 
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATTTT TGAGGTCAAA 
TCAACAGCTG GAGACACCCA CTTAGGTGGA GAAGATTTTG ACAACCGAAT GGTCAATCAT 
TTCATTGCTG AGTTCAAGCG AAAGCACAAG AAAGACATCA GTGAGAACAA GAGAGCTGTC 
CGCCGTCTCC GCACGGCCTG CGAGCGGGCC AAGCGCACCC TCTCCTCCAG CACCCAGGCC 
AGTATTGAGA TTGATTCTCT CTATGAGGGA ATTGACTTCT ATACCTCCAT TACCCGGGCT 
CGATTTGAGG AGTTGAATGC TGACCTGTTC CGTGGCACAC TGGACCCTGT AGAGAAGGCC 
CTTCGAGATG CCAAGCTGGA CAAGTCACAG ATCCATGATA TTGTCTTGGT GGGTGGTTCT 
ACCAGAATCC CCAAGATTCA GAAACTTCTG CAAGACTTCT TCAATGGAAA AGAGCTGAAC 
AAGAGCATTA ACCCCGATGA AGCTGTTGCC TATGGTGCAG CTGTCCAGGC AGCCATTCTA 
TCTGGAGACA AGTCTGAGAA CGTTCAGGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC 
CTTGGTATTG AAACTGCTGG CGGAGTCATG ACTGTCCTCA TCAAGCGCAA TACCACCATC 
CCCACCAAGC AGACACAGAC TTTCACCACC TACTCTGACA ACCAGCCTGG TGTACTCATT 
CAGGTGTATG AAGGTGAAAG GGCCATGACC AAGGACAACA ACCTGCTTGG AAAGTTCGAG 
CTCACAGGCA TCCCTCCAGC ACCCCGTGGG GTCCCTCAGA TTGAGGTTAC TTTTGACATC 
GATGCCAATG GCATCCTCAA TGTTTCTGCT GTAGATAAGA GCACAGGAAA GGAGAACAAG 
ATCACCATCA CCAATGACAA GGGCCGCTTG AGTAAGGAAG ATATTGAGCG CATGGTCCAA 
GAAGCTGAGA AGTACAAGGC TGAGGATGAG AAGCAGAGAG ATAAGGTTTC CTCCAAGAAC 
TCACTGGAGT CCTATGCCTT CAACATGAAA GCAACTGTGG AAGATGAGAA ACTTCAAGGC 
AAGATCAATG ATGAGGACAA ACAGAAGATT CTTGACAAGT GCAATGAAAT CATCAGCTGG 
CTGGATAAGA ACCAGACTGC AGAGAAGGAA GAATTTGAGC ATCAGCAGAA AGAACTGGAG 
AAAGTCTGCA ACCCTATCAT TACCAAGCTG TACCAGAGTG CAGGTGGCAT GCCTGGGGGA 
ATGCCTGGTG GCTTCCCAGG TGGAGGAGCT CCCCCATCTG GTGGTGCTTC TTCAGGCCCC 
ACCATTGAAG AGGTGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa8 ( NM_001364480.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001351409.1 Mus musculus heat shock protein 8 (Hspa8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83985
Clone ID Related Accession (Same CDS sequence)	NM_001364480.1
Accession Version	NM_001364480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	heat shock cognate 71 kDa protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158355.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094900.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTAAGG GACCTGCAGT TGGCATTGAT CTCGGCACCA CCTACTCCTG TGTGGGTGTC 
TTCCAGCATG GAAAGGTGGA AATTATTGCC AATGACCAGG GTAACCGCAC CACGCCAAGC 
TATGTTGCTT TCACGGACAC AGAGAGATTA ATTGGGGATG CGGCCAAGAA TCAGGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TCGGGCGTAG GTTTGATGAT 
GCTGTTGTTC AGTCTGATAT GAAGCACTGG CCCTTCATGG TGGTGAATGA TGCAGGCAGG 
CCCAAGGTCC AAGTGGAATA CAAAGGGGAG ACAAAAAGTT TCTACCCAGA GGAAGTGTCC 
TCCATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCGT ACCTCGGAAA GGCAACAAAA 
GATGCTGGAA CTATTGCTGG CCTCAATGTA CTTCGAATCA TCAATGAACC AACTGCTGCT 
GCTATTGCTT ACGGCTTAGA TAAGAAGGTC GGAGCTGAAA GGAATGTGCT CATTTTTGAC 
TTGGGAGGTG GCACTTTTGA TGTGTCAATC CTCACTATTG AGGATGGAAT TTTTGAGGTC 
AAATCAACAG CTGGAGACAC CCACTTAGGT GGAGAAGATT TTGACAACCG AATGGTCAAT 
CATTTCATTG CTGAGTTCAA GCGAAAGCAC AAGAAAGACA TCAGTGAGAA CAAGAGAGCT 
GTCCGCCGTC TCCGCACGGC CTGCGAGCGG GCCAAGCGCA CCCTCTCCTC CAGCACCCAG 
GCCAGTATTG AGATTGATTC TCTCTATGAG GGAATTGACT TCTATACCTC CATTACCCGG 
GCTCGATTTG AGGAGTTGAA TGCTGACCTG TTCCGTGGCA CACTGGACCC TGTAGAGAAG 
GCCCTTCGAG ATGCCAAGCT GGACAAGTCA CAGATCCATG ATATTGTCTT GGTGGGTGGT 
TCTACCAGAA TCCCCAAGAT TCAGAAACTT CTGCAAGACT TCTTCAATGG AAAAGAGCTG 
AACAAGAGCA TTAACCCCGA TGAAGCTGTT GCCTATGGTG CAGCTGTCCA GGCAGCCATT 
CTATCTGGAG ACAAGTCTGA GAACGTTCAG GATTTGCTGC TCTTGGATGT CACTCCTCTT 
TCCCTTGGTA TTGAAACTGC TGGCGGAGTC ATGACTGTCC TCATCAAGCG CAATACCACC 
ATCCCCACCA AGCAGACACA GACTTTCACC ACCTACTCTG ACAACCAGCC TGGTGTACTC 
ATTCAGGTGT ATGAAGGTGA AAGGGCCATG ACCAAGGACA ACAACCTGCT TGGAAAGTTC 
GAGCTCACAG GCATCCCTCC AGCACCCCGT GGGGTCCCTC AGATTGAGGT TACTTTTGAC 
ATCGATGCCA ATGGCATCCT CAATGTTTCT GCTGTAGATA AGAGCACAGG AAAGGAGAAC 
AAGATCACCA TCACCAATGA CAAGGGCCGC TTGAGTAAGG AAGATATTGA GCGCATGGTC 
CAAGAAGCTG AGAAGTACAA GGCTGAGGAT GAGAAGCAGA GAGATAAGGT TTCCTCCAAG 
AACTCACTGG AGTCCTATGC CTTCAACATG AAAGCAACTG TGGAAGATGA GAAACTTCAA 
GGCAAGATCA ATGATGAGGA CAAACAGAAG ATTCTTGACA AGTGCAATGA AATCATCAGC 
TGGCTGGATA AGAACCAGAC TGCAGAGAAG GAAGAATTTG AGCATCAGCA GAAAGAACTG 
GAGAAAGTCT GCAACCCTAT CATTACCAAG CTGTACCAGA GTGCAGGTGG CATGCCTGGG 
GGAATGCCTG GTGGCTTCCC AGGTGGAGGA GCTCCCCCAT CTGGTGGTGC TTCTTCAGGC 
CCCACCATTG AAGAGGTGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351409.1
CDS	79..1962
Translation	MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIISWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

Target ORF information:

RefSeq Version	NM_001364480.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 8 (Hspa8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364480.1

ORF Insert Sequence:

ATGTCTAAGG GACCTGCAGT TGGCATTGAT CTCGGCACCA CCTACTCCTG TGTGGGTGTC 
TTCCAGCATG GAAAGGTGGA AATTATTGCC AATGACCAGG GTAACCGCAC CACGCCAAGC 
TATGTTGCTT TCACGGACAC AGAGAGATTA ATTGGGGATG CGGCCAAGAA TCAGGTTGCA 
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TCGGGCGTAG GTTTGATGAT 
GCTGTTGTTC AGTCTGATAT GAAGCACTGG CCCTTCATGG TGGTGAATGA TGCAGGCAGG 
CCCAAGGTCC AAGTGGAATA CAAAGGGGAG ACAAAAAGTT TCTACCCAGA GGAAGTGTCC 
TCCATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCGT ACCTCGGAAA GGCAACAAAA 
GATGCTGGAA CTATTGCTGG CCTCAATGTA CTTCGAATCA TCAATGAACC AACTGCTGCT 
GCTATTGCTT ACGGCTTAGA TAAGAAGGTC GGAGCTGAAA GGAATGTGCT CATTTTTGAC 
TTGGGAGGTG GCACTTTTGA TGTGTCAATC CTCACTATTG AGGATGGAAT TTTTGAGGTC 
AAATCAACAG CTGGAGACAC CCACTTAGGT GGAGAAGATT TTGACAACCG AATGGTCAAT 
CATTTCATTG CTGAGTTCAA GCGAAAGCAC AAGAAAGACA TCAGTGAGAA CAAGAGAGCT 
GTCCGCCGTC TCCGCACGGC CTGCGAGCGG GCCAAGCGCA CCCTCTCCTC CAGCACCCAG 
GCCAGTATTG AGATTGATTC TCTCTATGAG GGAATTGACT TCTATACCTC CATTACCCGG 
GCTCGATTTG AGGAGTTGAA TGCTGACCTG TTCCGTGGCA CACTGGACCC TGTAGAGAAG 
GCCCTTCGAG ATGCCAAGCT GGACAAGTCA CAGATCCATG ATATTGTCTT GGTGGGTGGT 
TCTACCAGAA TCCCCAAGAT TCAGAAACTT CTGCAAGACT TCTTCAATGG AAAAGAGCTG 
AACAAGAGCA TTAACCCCGA TGAAGCTGTT GCCTATGGTG CAGCTGTCCA GGCAGCCATT 
CTATCTGGAG ACAAGTCTGA GAACGTTCAG GATTTGCTGC TCTTGGATGT CACTCCTCTT 
TCCCTTGGTA TTGAAACTGC TGGCGGAGTC ATGACTGTCC TCATCAAGCG CAATACCACC 
ATCCCCACCA AGCAGACACA GACTTTCACC ACCTACTCTG ACAACCAGCC TGGTGTACTC 
ATTCAGGTGT ATGAAGGTGA AAGGGCCATG ACCAAGGACA ACAACCTGCT TGGAAAGTTC 
GAGCTCACAG GCATCCCTCC AGCACCCCGT GGGGTCCCTC AGATTGAGGT TACTTTTGAC 
ATCGATGCCA ATGGCATCCT CAATGTTTCT GCTGTAGATA AGAGCACAGG AAAGGAGAAC 
AAGATCACCA TCACCAATGA CAAGGGCCGC TTGAGTAAGG AAGATATTGA GCGCATGGTC 
CAAGAAGCTG AGAAGTACAA GGCTGAGGAT GAGAAGCAGA GAGATAAGGT TTCCTCCAAG 
AACTCACTGG AGTCCTATGC CTTCAACATG AAAGCAACTG TGGAAGATGA GAAACTTCAA 
GGCAAGATCA ATGATGAGGA CAAACAGAAG ATTCTTGACA AGTGCAATGA AATCATCAGC 
TGGCTGGATA AGAACCAGAC TGCAGAGAAG GAAGAATTTG AGCATCAGCA GAAAGAACTG 
GAGAAAGTCT GCAACCCTAT CATTACCAAG CTGTACCAGA GTGCAGGTGG CATGCCTGGG 
GGAATGCCTG GTGGCTTCCC AGGTGGAGGA GCTCCCCCAT CTGGTGGTGC TTCTTCAGGC 
CCCACCATTG AAGAGGTGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Post-transcriptional Inhibition of Hsc70-4/HSPA8 Expression Leads to Synaptic Vesicle Cycling Defects in Multiple Models of ALS.
Cell reports21(1)110-125(2017 Oct)
Coyne AN,Lorenzini I,Chou CC,Torvund M,Rogers RS,Starr A,Zaepfel BL,Levy J,Johannesmeyer J,Schwartz JC,Nishimune H,Zinsmaier K,Rossoll W,Sattler R,Zarnescu DC

Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin.
The Journal of cell biology216(7)2059-2074(2017 Jul)
Ganguly A,Han X,Das U,Wang L,Loi J,Sun J,Gitler D,Caillol G,Leterrier C,Yates JR 3rd,Roy S

Salmonella Co-opts Host Cell Chaperone-mediated Autophagy for Intracellular Growth.
The Journal of biological chemistry292(5)1847-1864(2017 02)
Singh V,Finke-Isami J,Hopper-Chidlaw AC,Schwerk P,Thompson A,Tedin K

Functional analysis of HSPA1A and HSPA8 in parturition.
Biochemical and biophysical research communications483(1)371-379(2017 01)
Geng J,Li H,Huang C,Chai J,Zheng R,Li F,Jiang S

Subcellular distribution of non-muscle myosin IIb is controlled by FILIP through Hsc70.
PloS one12(2)e0172257(2017)
Yagi H,Takabayashi T,Xie MJ,Kuroda K,Sato M

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Hspa8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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