Prmt2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prmt2
Entrez Gene ID	15468
Full Name	protein arginine N-methyltransferase 2
Synonyms	AI504737,Hrmt1l1
General protein information	Preferred Names protein arginine N-methyltransferase 2 Names protein arginine N-methyltransferase 2 heterogeneous nuclear ribonucleoprotein methyltransferase-like 1 histone-arginine N-methyltransferase PRMT2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 C1\|10 38.74 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001302965.1	NP_001289894.1	protein arginine N-methyltransferase 2 isoform 1
NM_001077638.2	NP_001071106.1	protein arginine N-methyltransferase 2 isoform 2
NM_133182.3	NP_573445.2	protein arginine N-methyltransferase 2 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRMT2	NP_001526.2
Xenopus tropicalis (tropical clawed frog)	prmt2	NP_001072706.2
Pan troglodytes (chimpanzee)	PRMT2	XP_514952.2
Canis lupus familiaris (dog)	PRMT2	XP_005639087.1
Danio rerio (zebrafish)	prmt2	NP_001073644.1
	PRMT2	XP_001097529.1
Mus musculus (house mouse)	Prmt2	NP_573445.2
Rattus norvegicus (Norway rat)	Prmt2	NP_001020315.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural studies of protein arginine methyltransferase 2 reveal its interactions with potential substrates and inhibitors.
Cura V, Marechal N, Troffer-Charlier N, Strub JM, van Haren MJ, Martin NI, Cianf?rani S, Bonnefond L, Cavarelli J
The FEBS journal284(1)77-96(2017 01)

LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2.
Hussein MA, Shrestha E, Ouimet M, Barrett TJ, Leone S, Moore KJ, H?rault Y, Fisher EA, Garabedian MJ
PloS one10(8)e0135218(2015)

Novel skin phenotypes revealed by a genome-wide mouse reverse genetic screen.
Liakath-Ali K, Vancollie VE, Heath E, Smedley DP, Estabel J, Sunter D, Ditommaso T, White JK, Ramirez-Solis R, Smyth I, Steel KP, Watt FM
Nature communications53540(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PRMT2 is a modular protein containing a catalytic Ado-Met-binding domain and unique Src homology 3 domain that binds proteins with proline-rich motifs.
Title: Structural studies of protein arginine methyltransferase 2 reveal its interactions with potential substrates and inhibitors.

PRMT2 represents a glucose-sensitive factor that plays a role in Liver X Receptor-mediated ATP-binding cassette transporter A1-dependent cholesterol efflux and lends insight to the presence of increased atherosclerosis in diabetic patients.
Title: LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2.

Prmt2 is a new member of the NF-kappaB pathway controlling lipopolysaccharide (LPS)-induced inflammatory lung and macrophage responses in a dosage-dependent manner.
Title: Prmt2 regulates the lipopolysaccharide-induced responses in lungs and macrophages.

this study suggesting that the Cstb-Prmt2 region is not playing a major role in locomotor and cognitive deficits found in mice model of Down syndrome.
Title: The telomeric part of the human chromosome 21 from Cstb to Prmt2 is not necessary for the locomotor and short-term memory deficits observed in the Tc1 mouse model of Down syndrome.

data elucidate a molecular pathway that directly links arginine methylation of STAT3 by PRMT2 to the regulation of leptin signaling
Title: Disruption of protein arginine N-methyltransferase 2 regulates leptin signaling and produces leanness in vivo through loss of STAT3 methylation.

The following Prmt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prmt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13052	NM_001077638.2 Latest version!	Mus musculus protein arginine N-methyltransferase 2 (Prmt2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu13052	NM_133182.3 Latest version!	Mus musculus protein arginine N-methyltransferase 2 (Prmt2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu67967	NM_001302965.1 Latest version!	Mus musculus protein arginine N-methyltransferase 2 (Prmt2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prmt2 ( NM_001077638.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001071106.1 Mus musculus protein arginine N-methyltransferase 2 (Prmt2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13052
Clone ID Related Accession (Same CDS sequence)	NM_001077638.2 , NM_133182.3
Accession Version	NM_001077638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	protein arginine N-methyltransferase 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY288156.1, AK144412.1, AK159186.1 and AI504737.1. On Dec 11, 2014 this sequence version replaced NM_001077638.1. Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant 3. This results in isoform 2, which is shorter than isoform 1. Variants 1 and 2 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCAC CAGGAGAAGG TCCCTGCAGC GAGTCACAGG TCATCCCGGT TCTGGAGGAA 
GACCCCGTGG ACTATGGCTG TGAGATGCAG CTCCTGCAGG ATGGGGCACA GTTACAGCTG 
CAGCTGCAAC CTGAGGAGTT CGTGGCCATT GCAGACTATA CGGCCACCGA TGAGACACAG 
CTCAGCTTTT TGAGAGGAGA AAAAATTCTT ATCCTGAGAC AAACCACGGC GGACTGGTGG 
TGGGGCGAGC GCGCAGGCTG CTGTGGTTAC ATCCCTGCGA ACCACCTCGG CAAGCAACTG 
GAGGAATACG ACCCGGAGGA CACCTGGCAG GACGAGGAGT ACTTCGACAG CTATGGAACT 
CTGAAACTTC ACCTGGAAAT GTTGGCTGAC CAGCCTCGGA CCACCAAGTA CCACAGCGTC 
ATCCTGCAGA ACAAGGAGTC CCTGAAGGAC AAGGTCATCC TGGATGTGGG CTGCGGCACC 
GGCATCATCA GCCTCTTCTG CGCACACCAT GCCCGGCCCA AGGCGGTCTA CGCAGTGGAG 
GCCAGTGACA TGGCCCAGCA CACCAGCCAG CTGGTCCTGC AGAATGGCTT TGCTGACACA 
ATCACTGTGT TCCAGCAGAA GGTGGAGGAC GTGGTGCTGC CCGAGAAGGT GGACGTGCTG 
GTGTCTGAGT GGATGGGGAC CTGTCTGCTG TTTGAGTTCA TGATCGAGTC CATCCTGTAT 
GCCCGGGACA CATGGTTAAA GGGGGACGGC ATCATCTGGC CAACCACGGC CGCACTGCAT 
CTGGTGCCCT GCAGCGCCGA GAAAGACTAC CACAGCAAGG TGCTCTTCTG GGACAACGCG 
TACGAGTTCA ACCTCAGTGC ACTCAAGTCT TTAGCAATCA AGGAGTTCTT TTCAAGGCCA 
AAGTCGAATC ATATCTTGAA GCCAGAAGAC TGTCTCTCTG AGCCATGCAC AATCTTGCAG 
CTGGACATGA GAACTGTGCA GGTCCCTGAC CTGGAGACCA TGCGTGGCGA ACTGCGATTT 
GACATCCAGA AGGCTGGGAC CCTGCACGGC TTCACAGCTT GGTTCAGTGT GTACTTCCAG 
AGCCTGGAGG AGGGCCAGCC TCAGCAGGTG CTCAGCACAG GCCCGCTGCA CCCCACCACA 
CACTGGAAGC AGACCTTGTT CATGATGGAC GACCCAGTCC CGGTCCACAC GGGAGATGTG 
GTCACGGGTT CTGTTGTGTT ACAAAGAAAT CCGGTATGGA GAAGGCACAT GTCTGTCTCT 
CTGAGCTGGG TTGTCACGTC CGCACTCGAT CCCACGTCTC AAAGAGTTGG AGAGAAGGTC 
TTTCCGATCT GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071106.1
CDS	176..1513
Translation	MEAPGEGPCSESQVIPVLEEDPVDYGCEMQLLQDGAQLQLQLQPEEFVAIADYTATDETQLSFLRGEKILILRQTTADWWWGERAGCCGYIPANHLGKQLEEYDPEDTWQDEEYFDSYGTLKLHLEMLADQPRTTKYHSVILQNKESLKDKVILDVGCGTGIISLFCAHHARPKAVYAVEASDMAQHTSQLVLQNGFADTITVFQQKVEDVVLPEKVDVLVSEWMGTCLLFEFMIESILYARDTWLKGDGIIWPTTAALHLVPCSAEKDYHSKVLFWDNAYEFNLSALKSLAIKEFFSRPKSNHILKPEDCLSEPCTILQLDMRTVQVPDLETMRGELRFDIQKAGTLHGFTAWFSVYFQSLEEGQPQQVLSTGPLHPTTHWKQTLFMMDDPVPVHTGDVVTGSVVLQRNPVWRRHMSVSLSWVVTSALDPTSQRVGEKVFPIWR

Target ORF information:

RefSeq Version	NM_001077638.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein arginine N-methyltransferase 2 (Prmt2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077638.2

ORF Insert Sequence:

ATGGAGGCAC CAGGAGAAGG TCCCTGCAGC GAGTCACAGG TCATCCCGGT TCTGGAGGAA 
GACCCCGTGG ACTATGGCTG TGAGATGCAG CTCCTGCAGG ATGGGGCACA GTTACAGCTG 
CAGCTGCAAC CTGAGGAGTT CGTGGCCATT GCAGACTATA CGGCCACCGA TGAGACACAG 
CTCAGCTTTT TGAGAGGAGA AAAAATTCTT ATCCTGAGAC AAACCACGGC GGACTGGTGG 
TGGGGCGAGC GCGCAGGCTG CTGTGGTTAC ATCCCTGCGA ACCACCTCGG CAAGCAACTG 
GAGGAATACG ACCCGGAGGA CACCTGGCAG GACGAGGAGT ACTTCGACAG CTATGGAACT 
CTGAAACTTC ACCTGGAAAT GTTGGCTGAC CAGCCTCGGA CCACCAAGTA CCACAGCGTC 
ATCCTGCAGA ACAAGGAGTC CCTGAAGGAC AAGGTCATCC TGGATGTGGG CTGCGGCACC 
GGCATCATCA GCCTCTTCTG CGCACACCAT GCCCGGCCCA AGGCGGTCTA CGCAGTGGAG 
GCCAGTGACA TGGCCCAGCA CACCAGCCAG CTGGTCCTGC AGAATGGCTT TGCTGACACA 
ATCACTGTGT TCCAGCAGAA GGTGGAGGAC GTGGTGCTGC CCGAGAAGGT GGACGTGCTG 
GTGTCTGAGT GGATGGGGAC CTGTCTGCTG TTTGAGTTCA TGATCGAGTC CATCCTGTAT 
GCCCGGGACA CATGGTTAAA GGGGGACGGC ATCATCTGGC CAACCACGGC CGCACTGCAT 
CTGGTGCCCT GCAGCGCCGA GAAAGACTAC CACAGCAAGG TGCTCTTCTG GGACAACGCG 
TACGAGTTCA ACCTCAGTGC ACTCAAGTCT TTAGCAATCA AGGAGTTCTT TTCAAGGCCA 
AAGTCGAATC ATATCTTGAA GCCAGAAGAC TGTCTCTCTG AGCCATGCAC AATCTTGCAG 
CTGGACATGA GAACTGTGCA GGTCCCTGAC CTGGAGACCA TGCGTGGCGA ACTGCGATTT 
GACATCCAGA AGGCTGGGAC CCTGCACGGC TTCACAGCTT GGTTCAGTGT GTACTTCCAG 
AGCCTGGAGG AGGGCCAGCC TCAGCAGGTG CTCAGCACAG GCCCGCTGCA CCCCACCACA 
CACTGGAAGC AGACCTTGTT CATGATGGAC GACCCAGTCC CGGTCCACAC GGGAGATGTG 
GTCACGGGTT CTGTTGTGTT ACAAAGAAAT CCGGTATGGA GAAGGCACAT GTCTGTCTCT 
CTGAGCTGGG TTGTCACGTC CGCACTCGAT CCCACGTCTC AAAGAGTTGG AGAGAAGGTC 
TTTCCGATCT GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prmt2 ( NM_133182.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_573445.2 Mus musculus protein arginine N-methyltransferase 2 (Prmt2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13052
Clone ID Related Accession (Same CDS sequence)	NM_001077638.2 , NM_133182.3
Accession Version	NM_133182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	protein arginine N-methyltransferase 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY288156.1, BY727303.1, AK159186.1 and AI504737.1. On Dec 11, 2014 this sequence version replaced NM_133182.2. Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 3' coding region, compared to variant 3. This results in isoform 2, which is shorter than isoform 1. Variants 1 and 2 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCAC CAGGAGAAGG TCCCTGCAGC GAGTCACAGG TCATCCCGGT TCTGGAGGAA 
GACCCCGTGG ACTATGGCTG TGAGATGCAG CTCCTGCAGG ATGGGGCACA GTTACAGCTG 
CAGCTGCAAC CTGAGGAGTT CGTGGCCATT GCAGACTATA CGGCCACCGA TGAGACACAG 
CTCAGCTTTT TGAGAGGAGA AAAAATTCTT ATCCTGAGAC AAACCACGGC GGACTGGTGG 
TGGGGCGAGC GCGCAGGCTG CTGTGGTTAC ATCCCTGCGA ACCACCTCGG CAAGCAACTG 
GAGGAATACG ACCCGGAGGA CACCTGGCAG GACGAGGAGT ACTTCGACAG CTATGGAACT 
CTGAAACTTC ACCTGGAAAT GTTGGCTGAC CAGCCTCGGA CCACCAAGTA CCACAGCGTC 
ATCCTGCAGA ACAAGGAGTC CCTGAAGGAC AAGGTCATCC TGGATGTGGG CTGCGGCACC 
GGCATCATCA GCCTCTTCTG CGCACACCAT GCCCGGCCCA AGGCGGTCTA CGCAGTGGAG 
GCCAGTGACA TGGCCCAGCA CACCAGCCAG CTGGTCCTGC AGAATGGCTT TGCTGACACA 
ATCACTGTGT TCCAGCAGAA GGTGGAGGAC GTGGTGCTGC CCGAGAAGGT GGACGTGCTG 
GTGTCTGAGT GGATGGGGAC CTGTCTGCTG TTTGAGTTCA TGATCGAGTC CATCCTGTAT 
GCCCGGGACA CATGGTTAAA GGGGGACGGC ATCATCTGGC CAACCACGGC CGCACTGCAT 
CTGGTGCCCT GCAGCGCCGA GAAAGACTAC CACAGCAAGG TGCTCTTCTG GGACAACGCG 
TACGAGTTCA ACCTCAGTGC ACTCAAGTCT TTAGCAATCA AGGAGTTCTT TTCAAGGCCA 
AAGTCGAATC ATATCTTGAA GCCAGAAGAC TGTCTCTCTG AGCCATGCAC AATCTTGCAG 
CTGGACATGA GAACTGTGCA GGTCCCTGAC CTGGAGACCA TGCGTGGCGA ACTGCGATTT 
GACATCCAGA AGGCTGGGAC CCTGCACGGC TTCACAGCTT GGTTCAGTGT GTACTTCCAG 
AGCCTGGAGG AGGGCCAGCC TCAGCAGGTG CTCAGCACAG GCCCGCTGCA CCCCACCACA 
CACTGGAAGC AGACCTTGTT CATGATGGAC GACCCAGTCC CGGTCCACAC GGGAGATGTG 
GTCACGGGTT CTGTTGTGTT ACAAAGAAAT CCGGTATGGA GAAGGCACAT GTCTGTCTCT 
CTGAGCTGGG TTGTCACGTC CGCACTCGAT CCCACGTCTC AAAGAGTTGG AGAGAAGGTC 
TTTCCGATCT GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573445.2
CDS	173..1510
Translation	MEAPGEGPCSESQVIPVLEEDPVDYGCEMQLLQDGAQLQLQLQPEEFVAIADYTATDETQLSFLRGEKILILRQTTADWWWGERAGCCGYIPANHLGKQLEEYDPEDTWQDEEYFDSYGTLKLHLEMLADQPRTTKYHSVILQNKESLKDKVILDVGCGTGIISLFCAHHARPKAVYAVEASDMAQHTSQLVLQNGFADTITVFQQKVEDVVLPEKVDVLVSEWMGTCLLFEFMIESILYARDTWLKGDGIIWPTTAALHLVPCSAEKDYHSKVLFWDNAYEFNLSALKSLAIKEFFSRPKSNHILKPEDCLSEPCTILQLDMRTVQVPDLETMRGELRFDIQKAGTLHGFTAWFSVYFQSLEEGQPQQVLSTGPLHPTTHWKQTLFMMDDPVPVHTGDVVTGSVVLQRNPVWRRHMSVSLSWVVTSALDPTSQRVGEKVFPIWR

Target ORF information:

RefSeq Version	NM_133182.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein arginine N-methyltransferase 2 (Prmt2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133182.3

ORF Insert Sequence:

ATGGAGGCAC CAGGAGAAGG TCCCTGCAGC GAGTCACAGG TCATCCCGGT TCTGGAGGAA 
GACCCCGTGG ACTATGGCTG TGAGATGCAG CTCCTGCAGG ATGGGGCACA GTTACAGCTG 
CAGCTGCAAC CTGAGGAGTT CGTGGCCATT GCAGACTATA CGGCCACCGA TGAGACACAG 
CTCAGCTTTT TGAGAGGAGA AAAAATTCTT ATCCTGAGAC AAACCACGGC GGACTGGTGG 
TGGGGCGAGC GCGCAGGCTG CTGTGGTTAC ATCCCTGCGA ACCACCTCGG CAAGCAACTG 
GAGGAATACG ACCCGGAGGA CACCTGGCAG GACGAGGAGT ACTTCGACAG CTATGGAACT 
CTGAAACTTC ACCTGGAAAT GTTGGCTGAC CAGCCTCGGA CCACCAAGTA CCACAGCGTC 
ATCCTGCAGA ACAAGGAGTC CCTGAAGGAC AAGGTCATCC TGGATGTGGG CTGCGGCACC 
GGCATCATCA GCCTCTTCTG CGCACACCAT GCCCGGCCCA AGGCGGTCTA CGCAGTGGAG 
GCCAGTGACA TGGCCCAGCA CACCAGCCAG CTGGTCCTGC AGAATGGCTT TGCTGACACA 
ATCACTGTGT TCCAGCAGAA GGTGGAGGAC GTGGTGCTGC CCGAGAAGGT GGACGTGCTG 
GTGTCTGAGT GGATGGGGAC CTGTCTGCTG TTTGAGTTCA TGATCGAGTC CATCCTGTAT 
GCCCGGGACA CATGGTTAAA GGGGGACGGC ATCATCTGGC CAACCACGGC CGCACTGCAT 
CTGGTGCCCT GCAGCGCCGA GAAAGACTAC CACAGCAAGG TGCTCTTCTG GGACAACGCG 
TACGAGTTCA ACCTCAGTGC ACTCAAGTCT TTAGCAATCA AGGAGTTCTT TTCAAGGCCA 
AAGTCGAATC ATATCTTGAA GCCAGAAGAC TGTCTCTCTG AGCCATGCAC AATCTTGCAG 
CTGGACATGA GAACTGTGCA GGTCCCTGAC CTGGAGACCA TGCGTGGCGA ACTGCGATTT 
GACATCCAGA AGGCTGGGAC CCTGCACGGC TTCACAGCTT GGTTCAGTGT GTACTTCCAG 
AGCCTGGAGG AGGGCCAGCC TCAGCAGGTG CTCAGCACAG GCCCGCTGCA CCCCACCACA 
CACTGGAAGC AGACCTTGTT CATGATGGAC GACCCAGTCC CGGTCCACAC GGGAGATGTG 
GTCACGGGTT CTGTTGTGTT ACAAAGAAAT CCGGTATGGA GAAGGCACAT GTCTGTCTCT 
CTGAGCTGGG TTGTCACGTC CGCACTCGAT CCCACGTCTC AAAGAGTTGG AGAGAAGGTC 
TTTCCGATCT GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prmt2 ( NM_001302965.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289894.1 Mus musculus protein arginine N-methyltransferase 2 (Prmt2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu67967
Clone ID Related Accession (Same CDS sequence)	NM_001302965.1
Accession Version	NM_001302965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	protein arginine N-methyltransferase 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY288156.1, AK146966.1 and AI504737.1. Transcript Variant: This variant (3) represent the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCAC CAGGAGAAGG TCCCTGCAGC GAGTCACAGG TCATCCCGGT TCTGGAGGAA 
GACCCCGTGG ACTATGGCTG TGAGATGCAG CTCCTGCAGG ATGGGGCACA GTTACAGCTG 
CAGCTGCAAC CTGAGGAGTT CGTGGCCATT GCAGACTATA CGGCCACCGA TGAGACACAG 
CTCAGCTTTT TGAGAGGAGA AAAAATTCTT ATCCTGAGAC AAACCACGGC GGACTGGTGG 
TGGGGCGAGC GCGCAGGCTG CTGTGGTTAC ATCCCTGCGA ACCACCTCGG CAAGCAACTG 
GAGGAATACG ACCCGGAGGA CACCTGGCAG GACGAGGAGT ACTTCGACAG CTATGGAACT 
CTGAAACTTC ACCTGGAAAT GTTGGCTGAC CAGCCTCGGA CCACCAAGTA CCACAGCGTC 
ATCCTGCAGA ACAAGGAGTC CCTGAAGGAC AAGGTCATCC TGGATGTGGG CTGCGGCACC 
GGCATCATCA GCCTCTTCTG CGCACACCAT GCCCGGCCCA AGGCGGTCTA CGCAGTGGAG 
GCCAGTGACA TGGCCCAGCA CACCAGCCAG CTGGTCCTGC AGAATGGCTT TGCTGACACA 
ATCACTGTGT TCCAGCAGAA GGTGGAGGAC GTGGTGCTGC CCGAGAAGGT GGACGTGCTG 
GTGTCTGAGT GGATGGGGAC CTGTCTGCTG TTTGAGTTCA TGATCGAGTC CATCCTGTAT 
GCCCGGGACA CATGGTTAAA GGGGGACGGC ATCATCTGGC CAACCACGGC CGCACTGCAT 
CTGGTGCCCT GCAGCGCCGA GAAAGACTAC CACAGCAAGG TGCTCTTCTG GGACAACGCG 
TACGAGTTCA ACCTCAGTGC ACTCAAGTCT TTAGCAATCA AGGAGTTCTT TTCAAGGCCA 
AAGTCGAATC ATATCTTGAA GCCAGAAGAC TGTCTCTCTG AGCCATGCAC AATCTTGCAG 
CTGGACATGA GAACTGTGCA GGTCCCTGAC CTGGAGACCA TGCGTGGCGA ACTGCGATTT 
GACATCCAGA AGGCTGGGAC CCTGCACGGC TTCACAGCTT GGTTCAGTGT GTACTTCCAG 
AGCCTGGAGG AGGGCCAGCC TCAGCAGGTG CTCAGCACAG GCCCGCTGCA CCCGTTCCTG 
GGCAGGACAG GCTGTCAGTG CAGAGGACCT CGTTGGAGCT GCCAGATGAG GCCTGTAGGT 
GACCGCATGC TCCTGTCTTG CAGCACCACA CACTGGAAGC AGACCTTGTT CATGATGGAC 
GACCCAGTCC CGGTCCACAC GGGAGATGTG GTCACGGGTT CTGTTGTGTT ACAAAGAAAT 
CCGGTATGGA GAAGGCACAT GTCTGTCTCT CTGAGCTGGG TTGTCACGTC CGCACTCGAT 
CCCACGTCTC AAAGAGTTGG AGAGAAGGTC TTTCCGATCT GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289894.1
CDS	173..1600
Translation	MEAPGEGPCSESQVIPVLEEDPVDYGCEMQLLQDGAQLQLQLQPEEFVAIADYTATDETQLSFLRGEKILILRQTTADWWWGERAGCCGYIPANHLGKQLEEYDPEDTWQDEEYFDSYGTLKLHLEMLADQPRTTKYHSVILQNKESLKDKVILDVGCGTGIISLFCAHHARPKAVYAVEASDMAQHTSQLVLQNGFADTITVFQQKVEDVVLPEKVDVLVSEWMGTCLLFEFMIESILYARDTWLKGDGIIWPTTAALHLVPCSAEKDYHSKVLFWDNAYEFNLSALKSLAIKEFFSRPKSNHILKPEDCLSEPCTILQLDMRTVQVPDLETMRGELRFDIQKAGTLHGFTAWFSVYFQSLEEGQPQQVLSTGPLHPFLGRTGCQCRGPRWSCQMRPVGDRMLLSCSTTHWKQTLFMMDDPVPVHTGDVVTGSVVLQRNPVWRRHMSVSLSWVVTSALDPTSQRVGEKVFPIWR

Target ORF information:

RefSeq Version	NM_001302965.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein arginine N-methyltransferase 2 (Prmt2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302965.1

ORF Insert Sequence:

ATGGAGGCAC CAGGAGAAGG TCCCTGCAGC GAGTCACAGG TCATCCCGGT TCTGGAGGAA 
GACCCCGTGG ACTATGGCTG TGAGATGCAG CTCCTGCAGG ATGGGGCACA GTTACAGCTG 
CAGCTGCAAC CTGAGGAGTT CGTGGCCATT GCAGACTATA CGGCCACCGA TGAGACACAG 
CTCAGCTTTT TGAGAGGAGA AAAAATTCTT ATCCTGAGAC AAACCACGGC GGACTGGTGG 
TGGGGCGAGC GCGCAGGCTG CTGTGGTTAC ATCCCTGCGA ACCACCTCGG CAAGCAACTG 
GAGGAATACG ACCCGGAGGA CACCTGGCAG GACGAGGAGT ACTTCGACAG CTATGGAACT 
CTGAAACTTC ACCTGGAAAT GTTGGCTGAC CAGCCTCGGA CCACCAAGTA CCACAGCGTC 
ATCCTGCAGA ACAAGGAGTC CCTGAAGGAC AAGGTCATCC TGGATGTGGG CTGCGGCACC 
GGCATCATCA GCCTCTTCTG CGCACACCAT GCCCGGCCCA AGGCGGTCTA CGCAGTGGAG 
GCCAGTGACA TGGCCCAGCA CACCAGCCAG CTGGTCCTGC AGAATGGCTT TGCTGACACA 
ATCACTGTGT TCCAGCAGAA GGTGGAGGAC GTGGTGCTGC CCGAGAAGGT GGACGTGCTG 
GTGTCTGAGT GGATGGGGAC CTGTCTGCTG TTTGAGTTCA TGATCGAGTC CATCCTGTAT 
GCCCGGGACA CATGGTTAAA GGGGGACGGC ATCATCTGGC CAACCACGGC CGCACTGCAT 
CTGGTGCCCT GCAGCGCCGA GAAAGACTAC CACAGCAAGG TGCTCTTCTG GGACAACGCG 
TACGAGTTCA ACCTCAGTGC ACTCAAGTCT TTAGCAATCA AGGAGTTCTT TTCAAGGCCA 
AAGTCGAATC ATATCTTGAA GCCAGAAGAC TGTCTCTCTG AGCCATGCAC AATCTTGCAG 
CTGGACATGA GAACTGTGCA GGTCCCTGAC CTGGAGACCA TGCGTGGCGA ACTGCGATTT 
GACATCCAGA AGGCTGGGAC CCTGCACGGC TTCACAGCTT GGTTCAGTGT GTACTTCCAG 
AGCCTGGAGG AGGGCCAGCC TCAGCAGGTG CTCAGCACAG GCCCGCTGCA CCCGTTCCTG 
GGCAGGACAG GCTGTCAGTG CAGAGGACCT CGTTGGAGCT GCCAGATGAG GCCTGTAGGT 
GACCGCATGC TCCTGTCTTG CAGCACCACA CACTGGAAGC AGACCTTGTT CATGATGGAC 
GACCCAGTCC CGGTCCACAC GGGAGATGTG GTCACGGGTT CTGTTGTGTT ACAAAGAAAT 
CCGGTATGGA GAAGGCACAT GTCTGTCTCT CTGAGCTGGG TTGTCACGTC CGCACTCGAT 
CCCACGTCTC AAAGAGTTGG AGAGAAGGTC TTTCCGATCT GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural studies of protein arginine methyltransferase 2 reveal its interactions with potential substrates and inhibitors.
The FEBS journal284(1)77-96(2017 01)
Cura V,Marechal N,Troffer-Charlier N,Strub JM,van Haren MJ,Martin NI,Cianf?rani S,Bonnefond L,Cavarelli J

LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2.
PloS one10(8)e0135218(2015)
Hussein MA,Shrestha E,Ouimet M,Barrett TJ,Leone S,Moore KJ,H?rault Y,Fisher EA,Garabedian MJ

Novel skin phenotypes revealed by a genome-wide mouse reverse genetic screen.
Nature communications53540(2014 Apr)
Liakath-Ali K,Vancollie VE,Heath E,Smedley DP,Estabel J,Sunter D,Ditommaso T,White JK,Ramirez-Solis R,Smyth I,Steel KP,Watt FM

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Prmt2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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