Hpn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hpn
Entrez Gene ID	15451
Full Name	hepsin
Synonyms	Hlb320
General protein information	Preferred Names hepsin Names serine protease hepsin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B1
Summary	This gene encodes a type II transmembrane serine protease that may function in diverse processes, including regulation of cell growth. Deficiency in this gene results in hearing loss. The protein is cleaved into a catalytic serine protease chain and a non-catalytic scavenger receptor cysteine-rich chain, which associate via a single disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110252.2	NP_001103722.1	serine protease hepsin isoform 1 precursor
NM_008281.4	NP_032307.2	serine protease hepsin isoform 2 precursor
NM_001276269.1	NP_001263198.1	serine protease hepsin isoform 3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HPN	XP_001157575.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004552	XP_313850.5
Canis lupus familiaris (dog)	HPN	XP_541697.2
Bos taurus (cattle)	HPN	NP_001073710.1
Danio rerio (zebrafish)	si:dkey-33i11.3	NP_001091657.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008649	XP_314746.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004568	XP_313873.5
Homo sapiens (human)	HPN	NP_892028.1
Rattus norvegicus (Norway rat)	Hpn	NP_058808.1
Danio rerio (zebrafish)	LOC100536154	XP_003200307.1
	HPN	XP_001093699.2
Mus musculus (house mouse)	Hpn	NP_032307.2

Gene Ontology
Bibliography

Related articles in PubMed

The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin.
Brunati M, Perucca S, Han L, Cattaneo A, Consolato F, Andolfo A, Schaeffer C, Olinger E, Peng J, Santambrogio S, Perrier R, Li S, Bokhove M, Bachi A, Hummler E, Devuyst O, Wu Q, Jovine L, Rampoldi L
eLife4e08887(2015 Dec)

Serine protease hepsin regulates hepatocyte size and hemodynamic retention of tumor cells by hepatocyte growth factor signaling in mice.
Hsu YC, Huang HP, Yu IS, Su KY, Lin SR, Lin WC, Wu HL, Shi GY, Tao MH, Kao CH, Wu YM, Martin PE, Lin SY, Yang PC, Lin SW
Hepatology (Baltimore, Md.)56(5)1913-23(2012 Nov)

Pla2g12b and Hpn are genes identified by mouse ENU mutagenesis that affect HDL cholesterol.
Aljakna A, Choi S, Savage H, Hageman Blair R, Gu T, Svenson KL, Churchill GA, Hibbs M, Korstanje R
PloS one7(8)e43139(2012)

Thyroxine treatments do not correct inner ear defects in tmprss1 mutant mice.
Hanifa S, Scott HS, Crewther P, Guipponi M, Tan J
Neuroreport21(13)897-901(2010 Sep)

The structure of the extracellular region of human hepsin reveals a serine protease domain and a novel scavenger receptor cysteine-rich (SRCR) domain.
Somoza JR, Ho JD, Luong C, Ghate M, Sprengeler PA, Mortara K, Shrader WD, Sperandio D, Chan H, McGrath ME, Katz BA
Structure (London, England : 1993)11(9)1123-31(2003 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hepsin cooperates with MYC in the progression of adenocarcinoma in a prostate cancer mouse model.
Title: Hepsin cooperates with MYC in the progression of adenocarcinoma in a prostate cancer mouse model.

Ln-332 may be one mechanism by which hepsin promotes prostate tumor progression and metastasis, possibly by up-regulating prostate cancer cell motility.
Title: Laminin-332 is a substrate for hepsin, a protease associated with prostate cancer progression.

The ability of ectopic hepsin to induce tumor growth in mice is abrogated by the mutation of 3 critical residues in the catalytic domain, thus implicating the enzymatic activity of hepsin in promoting tumor progression.
Title: Hepsin colocalizes with desmosomes and induces progression of ovarian cancer in a mouse model.

Hearing impairment present in hepsin/Tmprss1-null mice is characterized by a combination of various structural, cellular, and molecular abnormalities that are likely to affect different cochlear processes.
Title: Mice deficient for the type II transmembrane serine protease, TMPRSS1/hepsin, exhibit profound hearing loss.

the ability of hepsin to efficiently activate pro-uPA suggests that it may initiate plasmin-mediated proteolytic pathways at the tumor/stroma interface that lead to basement membrane disruption and tumor progression
Title: Pro-urokinase-type plasminogen activator is a substrate for hepsin.

The following Hpn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hpn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01663	NM_008281.4 Latest version!	Mus musculus hepsin (Hpn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu04574	NM_001110252.2 Latest version!	Mus musculus hepsin (Hpn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$251.30 $359.00
OMu17036	NM_001276269.1 Latest version!	Mus musculus hepsin (Hpn), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hpn ( NM_008281.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032307.2 Mus musculus hepsin (Hpn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01663
Clone ID Related Accession (Same CDS sequence)	NM_008281.4
Accession Version	NM_008281.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	serine protease hepsin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158993.2, BC138809.1 and CJ237993.1. On Jan 18, 2013 this sequence version replaced NM_008281.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF030065.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGAAGG AGGGTGGCCG GACTGCAGCA TGCTGCTCCA GACCCAAGGT GGCAGCTCTC 
ATTGTGGGTA CCCTGCTGTT CCTGACAGGC ATTGGGGCCG CGTCCTGGGC CATTGTGACC 
ATCCTACTGC AGAGTGACCA GGAGCCACTG TACCAAGTGC AGCTCAGTCC AGGGGACTCA 
CGGCTTGCGG TGTTTGACAA GACGGAGGGA ACGTGGAGGC TACTGTGCTC CTCACGCTCC 
AATGCCAGGG TGGCAGGGCT CGGCTGTGAG GAGATGGGCT TTCTCAGGGC TCTGGCGCAC 
TCGGAGCTGG ATGTGCGCAC TGCGGGCGCC AACGGCACAT CGGGCTTCTT TTGCGTGGAC 
GAGGGCGGAC TGCCTCTGGC TCAGAGGTTG CTGGATGTCA TCTCTGTATG TGACTGTCCT 
AGAGGCCGAT TCCTGACTGC CACCTGCCAA GACTGTGGCC GCAGGAAGCT GCCGGTGGAC 
CGCATTGTGG GGGGCCAGGA CAGCAGTCTG GGAAGGTGGC CGTGGCAGGT CAGCCTGCGT 
TATGATGGGA CCCACCTCTG TGGGGGGTCC CTGCTGTCTG GGGACTGGGT GCTGACTGCT 
GCACATTGCT TTCCAGAGCG GAACCGGGTC CTGTCTCGGT GGCGAGTATT TGCTGGTGCT 
GTAGCCCGGA CCTCACCCCA TGCTGTGCAA CTGGGGGTTC AGGCTGTGAT CTATCATGGG 
GGCTACCTTC CCTTTCGAGA CCCTACTATC GACGAAAACA GCAATGACAT TGCCTTGGTC 
CACCTCTCTA GCTCCCTGCC TCTCACAGAA TACATCCAGC CAGTGTGTCT CCCTGCTGCG 
GGACAGGCCC TGGTGGATGG CAAGGTCTGT ACTGTGACCG GCTGGGGTAA CACACAGTTC 
TATGGCCAAC AGGCTATGGT GCTCCAAGAG GCCCGGGTTC CCATCATAAG CAACGAAGTT 
TGCAACAGCC CCGACTTCTA CGGGAATCAG ATCAAGCCCA AGATGTTCTG TGCTGGCTAT 
CCTGAGGGTG GCATTGATGC GTGCCAGGGC GACAGTGGAG GCCCCTTTGT GTGTGAAGAC 
AGCATCTCTG GGACATCAAG GTGGCGGCTA TGTGGCATTG TAAGCTGGGG TACGGGCTGT 
GCTTTGGCCC GGAAGCCAGG AGTGTACACC AAAGTCACTG ACTTCCGGGA GTGGATCTTC 
AAGGCCATAA AGACTCACTC CGAAGCCAGT GGCATGGTGA CTCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032307.2
CDS	246..1496
Translation	MAKEGGRTAACCSRPKVAALIVGTLLFLTGIGAASWAIVTILLQSDQEPLYQVQLSPGDSRLAVFDKTEGTWRLLCSSRSNARVAGLGCEEMGFLRALAHSELDVRTAGANGTSGFFCVDEGGLPLAQRLLDVISVCDCPRGRFLTATCQDCGRRKLPVDRIVGGQDSSLGRWPWQVSLRYDGTHLCGGSLLSGDWVLTAAHCFPERNRVLSRWRVFAGAVARTSPHAVQLGVQAVIYHGGYLPFRDPTIDENSNDIALVHLSSSLPLTEYIQPVCLPAAGQALVDGKVCTVTGWGNTQFYGQQAMVLQEARVPIISNEVCNSPDFYGNQIKPKMFCAGYPEGGIDACQGDSGGPFVCEDSISGTSRWRLCGIVSWGTGCALARKPGVYTKVTDFREWIFKAIKTHSEASGMVTQP

Target ORF information:

RefSeq Version	NM_008281.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hepsin (Hpn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008281.4

ORF Insert Sequence:

ATGGCGAAGG AGGGTGGCCG GACTGCAGCA TGCTGCTCCA GACCCAAGGT GGCAGCTCTC 
ATTGTGGGTA CCCTGCTGTT CCTGACAGGC ATTGGGGCCG CGTCCTGGGC CATTGTGACC 
ATCCTACTGC AGAGTGACCA GGAGCCACTG TACCAAGTGC AGCTCAGTCC AGGGGACTCA 
CGGCTTGCGG TGTTTGACAA GACGGAGGGA ACGTGGAGGC TACTGTGCTC CTCACGCTCC 
AATGCCAGGG TGGCAGGGCT CGGCTGTGAG GAGATGGGCT TTCTCAGGGC TCTGGCGCAC 
TCGGAGCTGG ATGTGCGCAC TGCGGGCGCC AACGGCACAT CGGGCTTCTT TTGCGTGGAC 
GAGGGCGGAC TGCCTCTGGC TCAGAGGTTG CTGGATGTCA TCTCTGTATG TGACTGTCCT 
AGAGGCCGAT TCCTGACTGC CACCTGCCAA GACTGTGGCC GCAGGAAGCT GCCGGTGGAC 
CGCATTGTGG GGGGCCAGGA CAGCAGTCTG GGAAGGTGGC CGTGGCAGGT CAGCCTGCGT 
TATGATGGGA CCCACCTCTG TGGGGGGTCC CTGCTGTCTG GGGACTGGGT GCTGACTGCT 
GCACATTGCT TTCCAGAGCG GAACCGGGTC CTGTCTCGGT GGCGAGTATT TGCTGGTGCT 
GTAGCCCGGA CCTCACCCCA TGCTGTGCAA CTGGGGGTTC AGGCTGTGAT CTATCATGGG 
GGCTACCTTC CCTTTCGAGA CCCTACTATC GACGAAAACA GCAATGACAT TGCCTTGGTC 
CACCTCTCTA GCTCCCTGCC TCTCACAGAA TACATCCAGC CAGTGTGTCT CCCTGCTGCG 
GGACAGGCCC TGGTGGATGG CAAGGTCTGT ACTGTGACCG GCTGGGGTAA CACACAGTTC 
TATGGCCAAC AGGCTATGGT GCTCCAAGAG GCCCGGGTTC CCATCATAAG CAACGAAGTT 
TGCAACAGCC CCGACTTCTA CGGGAATCAG ATCAAGCCCA AGATGTTCTG TGCTGGCTAT 
CCTGAGGGTG GCATTGATGC GTGCCAGGGC GACAGTGGAG GCCCCTTTGT GTGTGAAGAC 
AGCATCTCTG GGACATCAAG GTGGCGGCTA TGTGGCATTG TAAGCTGGGG TACGGGCTGT 
GCTTTGGCCC GGAAGCCAGG AGTGTACACC AAAGTCACTG ACTTCCGGGA GTGGATCTTC 
AAGGCCATAA AGACTCACTC CGAAGCCAGT GGCATGGTGA CTCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hpn ( NM_001110252.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001103722.1 Mus musculus hepsin (Hpn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu04574
Clone ID Related Accession (Same CDS sequence)	NM_001110252.2
Accession Version	NM_001110252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	serine protease hepsin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158993.2, BC138809.1 and CJ237993.1. On Jan 18, 2013 this sequence version replaced NM_001110252.1. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK002694.1, BC138809.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGAAGG AGGATGAGGA ACCTGGGGCT CACAGAGGAG GTTCCACTTG TTCAAGACCC 
CAACCTGGAA AGGGTGGCCG GACTGCAGCA TGCTGCTCCA GACCCAAGGT GGCAGCTCTC 
ATTGTGGGTA CCCTGCTGTT CCTGACAGGC ATTGGGGCCG CGTCCTGGGC CATTGTGACC 
ATCCTACTGC AGAGTGACCA GGAGCCACTG TACCAAGTGC AGCTCAGTCC AGGGGACTCA 
CGGCTTGCGG TGTTTGACAA GACGGAGGGA ACGTGGAGGC TACTGTGCTC CTCACGCTCC 
AATGCCAGGG TGGCAGGGCT CGGCTGTGAG GAGATGGGCT TTCTCAGGGC TCTGGCGCAC 
TCGGAGCTGG ATGTGCGCAC TGCGGGCGCC AACGGCACAT CGGGCTTCTT TTGCGTGGAC 
GAGGGCGGAC TGCCTCTGGC TCAGAGGTTG CTGGATGTCA TCTCTGTATG TGACTGTCCT 
AGAGGCCGAT TCCTGACTGC CACCTGCCAA GACTGTGGCC GCAGGAAGCT GCCGGTGGAC 
CGCATTGTGG GGGGCCAGGA CAGCAGTCTG GGAAGGTGGC CGTGGCAGGT CAGCCTGCGT 
TATGATGGGA CCCACCTCTG TGGGGGGTCC CTGCTGTCTG GGGACTGGGT GCTGACTGCT 
GCACATTGCT TTCCAGAGCG GAACCGGGTC CTGTCTCGGT GGCGAGTATT TGCTGGTGCT 
GTAGCCCGGA CCTCACCCCA TGCTGTGCAA CTGGGGGTTC AGGCTGTGAT CTATCATGGG 
GGCTACCTTC CCTTTCGAGA CCCTACTATC GACGAAAACA GCAATGACAT TGCCTTGGTC 
CACCTCTCTA GCTCCCTGCC TCTCACAGAA TACATCCAGC CAGTGTGTCT CCCTGCTGCG 
GGACAGGCCC TGGTGGATGG CAAGGTCTGT ACTGTGACCG GCTGGGGTAA CACACAGTTC 
TATGGCCAAC AGGCTATGGT GCTCCAAGAG GCCCGGGTTC CCATCATAAG CAACGAAGTT 
TGCAACAGCC CCGACTTCTA CGGGAATCAG ATCAAGCCCA AGATGTTCTG TGCTGGCTAT 
CCTGAGGGTG GCATTGATGC GTGCCAGGGC GACAGTGGAG GCCCCTTTGT GTGTGAAGAC 
AGCATCTCTG GGACATCAAG GTGGCGGCTA TGTGGCATTG TAAGCTGGGG TACGGGCTGT 
GCTTTGGCCC GGAAGCCAGG AGTGTACACC AAAGTCACTG ACTTCCGGGA GTGGATCTTC 
AAGGCCATAA AGACTCACTC CGAAGCCAGT GGCATGGTGA CTCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103722.1
CDS	246..1556
Translation	MAKEDEEPGAHRGGSTCSRPQPGKGGRTAACCSRPKVAALIVGTLLFLTGIGAASWAIVTILLQSDQEPLYQVQLSPGDSRLAVFDKTEGTWRLLCSSRSNARVAGLGCEEMGFLRALAHSELDVRTAGANGTSGFFCVDEGGLPLAQRLLDVISVCDCPRGRFLTATCQDCGRRKLPVDRIVGGQDSSLGRWPWQVSLRYDGTHLCGGSLLSGDWVLTAAHCFPERNRVLSRWRVFAGAVARTSPHAVQLGVQAVIYHGGYLPFRDPTIDENSNDIALVHLSSSLPLTEYIQPVCLPAAGQALVDGKVCTVTGWGNTQFYGQQAMVLQEARVPIISNEVCNSPDFYGNQIKPKMFCAGYPEGGIDACQGDSGGPFVCEDSISGTSRWRLCGIVSWGTGCALARKPGVYTKVTDFREWIFKAIKTHSEASGMVTQP

Target ORF information:

RefSeq Version	NM_001110252.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hepsin (Hpn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110252.2

ORF Insert Sequence:

ATGGCGAAGG AGGATGAGGA ACCTGGGGCT CACAGAGGAG GTTCCACTTG TTCAAGACCC 
CAACCTGGAA AGGGTGGCCG GACTGCAGCA TGCTGCTCCA GACCCAAGGT GGCAGCTCTC 
ATTGTGGGTA CCCTGCTGTT CCTGACAGGC ATTGGGGCCG CGTCCTGGGC CATTGTGACC 
ATCCTACTGC AGAGTGACCA GGAGCCACTG TACCAAGTGC AGCTCAGTCC AGGGGACTCA 
CGGCTTGCGG TGTTTGACAA GACGGAGGGA ACGTGGAGGC TACTGTGCTC CTCACGCTCC 
AATGCCAGGG TGGCAGGGCT CGGCTGTGAG GAGATGGGCT TTCTCAGGGC TCTGGCGCAC 
TCGGAGCTGG ATGTGCGCAC TGCGGGCGCC AACGGCACAT CGGGCTTCTT TTGCGTGGAC 
GAGGGCGGAC TGCCTCTGGC TCAGAGGTTG CTGGATGTCA TCTCTGTATG TGACTGTCCT 
AGAGGCCGAT TCCTGACTGC CACCTGCCAA GACTGTGGCC GCAGGAAGCT GCCGGTGGAC 
CGCATTGTGG GGGGCCAGGA CAGCAGTCTG GGAAGGTGGC CGTGGCAGGT CAGCCTGCGT 
TATGATGGGA CCCACCTCTG TGGGGGGTCC CTGCTGTCTG GGGACTGGGT GCTGACTGCT 
GCACATTGCT TTCCAGAGCG GAACCGGGTC CTGTCTCGGT GGCGAGTATT TGCTGGTGCT 
GTAGCCCGGA CCTCACCCCA TGCTGTGCAA CTGGGGGTTC AGGCTGTGAT CTATCATGGG 
GGCTACCTTC CCTTTCGAGA CCCTACTATC GACGAAAACA GCAATGACAT TGCCTTGGTC 
CACCTCTCTA GCTCCCTGCC TCTCACAGAA TACATCCAGC CAGTGTGTCT CCCTGCTGCG 
GGACAGGCCC TGGTGGATGG CAAGGTCTGT ACTGTGACCG GCTGGGGTAA CACACAGTTC 
TATGGCCAAC AGGCTATGGT GCTCCAAGAG GCCCGGGTTC CCATCATAAG CAACGAAGTT 
TGCAACAGCC CCGACTTCTA CGGGAATCAG ATCAAGCCCA AGATGTTCTG TGCTGGCTAT 
CCTGAGGGTG GCATTGATGC GTGCCAGGGC GACAGTGGAG GCCCCTTTGT GTGTGAAGAC 
AGCATCTCTG GGACATCAAG GTGGCGGCTA TGTGGCATTG TAAGCTGGGG TACGGGCTGT 
GCTTTGGCCC GGAAGCCAGG AGTGTACACC AAAGTCACTG ACTTCCGGGA GTGGATCTTC 
AAGGCCATAA AGACTCACTC CGAAGCCAGT GGCATGGTGA CTCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hpn ( NM_001276269.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001263198.1 Mus musculus hepsin (Hpn), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu17036
Clone ID Related Accession (Same CDS sequence)	NM_001276269.1
Accession Version	NM_001276269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	serine protease hepsin isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158993.2, BC138809.1 and CJ237993.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is longer and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK156553.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGTCG GAGCTAGAGA CGACGCTGGG CACATGGCAG ATGAGGAACC TGGGGCTCAC 
AGAGGAGGTT CCACTTGTTC AAGACCCCAA CCTGGAAAGG GTGGCCGGAC TGCAGCATGC 
TGCTCCAGAC CCAAGGTGGC AGCTCTCATT GTGGGTACCC TGCTGTTCCT GACAGGCATT 
GGGGCCGCGT CCTGGGCCAT TGTGACCATC CTACTGCAGA GTGACCAGGA GCCACTGTAC 
CAAGTGCAGC TCAGTCCAGG GGACTCACGG CTTGCGGTGT TTGACAAGAC GGAGGGAACG 
TGGAGGCTAC TGTGCTCCTC ACGCTCCAAT GCCAGGGTGG CAGGGCTCGG CTGTGAGGAG 
ATGGGCTTTC TCAGGGCTCT GGCGCACTCG GAGCTGGATG TGCGCACTGC GGGCGCCAAC 
GGCACATCGG GCTTCTTTTG CGTGGACGAG GGCGGACTGC CTCTGGCTCA GAGGTTGCTG 
GATGTCATCT CTGTATGTGA CTGTCCTAGA GGCCGATTCC TGACTGCCAC CTGCCAAGAC 
TGTGGCCGCA GGAAGCTGCC GGTGGACCGC ATTGTGGGGG GCCAGGACAG CAGTCTGGGA 
AGGTGGCCGT GGCAGGTCAG CCTGCGTTAT GATGGGACCC ACCTCTGTGG GGGGTCCCTG 
CTGTCTGGGG ACTGGGTGCT GACTGCTGCA CATTGCTTTC CAGAGCGGAA CCGGGTCCTG 
TCTCGGTGGC GAGTATTTGC TGGTGCTGTA GCCCGGACCT CACCCCATGC TGTGCAACTG 
GGGGTTCAGG CTGTGATCTA TCATGGGGGC TACCTTCCCT TTCGAGACCC TACTATCGAC 
GAAAACAGCA ATGACATTGC CTTGGTCCAC CTCTCTAGCT CCCTGCCTCT CACAGAATAC 
ATCCAGCCAG TGTGTCTCCC TGCTGCGGGA CAGGCCCTGG TGGATGGCAA GGTCTGTACT 
GTGACCGGCT GGGGTAACAC ACAGTTCTAT GGCCAACAGG CTATGGTGCT CCAAGAGGCC 
CGGGTTCCCA TCATAAGCAA CGAAGTTTGC AACAGCCCCG ACTTCTACGG GAATCAGATC 
AAGCCCAAGA TGTTCTGTGC TGGCTATCCT GAGGGTGGCA TTGATGCGTG CCAGGGCGAC 
AGTGGAGGCC CCTTTGTGTG TGAAGACAGC ATCTCTGGGA CATCAAGGTG GCGGCTATGT 
GGCATTGTAA GCTGGGGTAC GGGCTGTGCT TTGGCCCGGA AGCCAGGAGT GTACACCAAA 
GTCACTGACT TCCGGGAGTG GATCTTCAAG GCCATAAAGA CTCACTCCGA AGCCAGTGGC 
ATGGTGACTC AGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263198.1
CDS	96..1433
Translation	MWVGARDDAGHMADEEPGAHRGGSTCSRPQPGKGGRTAACCSRPKVAALIVGTLLFLTGIGAASWAIVTILLQSDQEPLYQVQLSPGDSRLAVFDKTEGTWRLLCSSRSNARVAGLGCEEMGFLRALAHSELDVRTAGANGTSGFFCVDEGGLPLAQRLLDVISVCDCPRGRFLTATCQDCGRRKLPVDRIVGGQDSSLGRWPWQVSLRYDGTHLCGGSLLSGDWVLTAAHCFPERNRVLSRWRVFAGAVARTSPHAVQLGVQAVIYHGGYLPFRDPTIDENSNDIALVHLSSSLPLTEYIQPVCLPAAGQALVDGKVCTVTGWGNTQFYGQQAMVLQEARVPIISNEVCNSPDFYGNQIKPKMFCAGYPEGGIDACQGDSGGPFVCEDSISGTSRWRLCGIVSWGTGCALARKPGVYTKVTDFREWIFKAIKTHSEASGMVTQP

Target ORF information:

RefSeq Version	NM_001276269.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hepsin (Hpn), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276269.1

ORF Insert Sequence:

ATGTGGGTCG GAGCTAGAGA CGACGCTGGG CACATGGCAG ATGAGGAACC TGGGGCTCAC 
AGAGGAGGTT CCACTTGTTC AAGACCCCAA CCTGGAAAGG GTGGCCGGAC TGCAGCATGC 
TGCTCCAGAC CCAAGGTGGC AGCTCTCATT GTGGGTACCC TGCTGTTCCT GACAGGCATT 
GGGGCCGCGT CCTGGGCCAT TGTGACCATC CTACTGCAGA GTGACCAGGA GCCACTGTAC 
CAAGTGCAGC TCAGTCCAGG GGACTCACGG CTTGCGGTGT TTGACAAGAC GGAGGGAACG 
TGGAGGCTAC TGTGCTCCTC ACGCTCCAAT GCCAGGGTGG CAGGGCTCGG CTGTGAGGAG 
ATGGGCTTTC TCAGGGCTCT GGCGCACTCG GAGCTGGATG TGCGCACTGC GGGCGCCAAC 
GGCACATCGG GCTTCTTTTG CGTGGACGAG GGCGGACTGC CTCTGGCTCA GAGGTTGCTG 
GATGTCATCT CTGTATGTGA CTGTCCTAGA GGCCGATTCC TGACTGCCAC CTGCCAAGAC 
TGTGGCCGCA GGAAGCTGCC GGTGGACCGC ATTGTGGGGG GCCAGGACAG CAGTCTGGGA 
AGGTGGCCGT GGCAGGTCAG CCTGCGTTAT GATGGGACCC ACCTCTGTGG GGGGTCCCTG 
CTGTCTGGGG ACTGGGTGCT GACTGCTGCA CATTGCTTTC CAGAGCGGAA CCGGGTCCTG 
TCTCGGTGGC GAGTATTTGC TGGTGCTGTA GCCCGGACCT CACCCCATGC TGTGCAACTG 
GGGGTTCAGG CTGTGATCTA TCATGGGGGC TACCTTCCCT TTCGAGACCC TACTATCGAC 
GAAAACAGCA ATGACATTGC CTTGGTCCAC CTCTCTAGCT CCCTGCCTCT CACAGAATAC 
ATCCAGCCAG TGTGTCTCCC TGCTGCGGGA CAGGCCCTGG TGGATGGCAA GGTCTGTACT 
GTGACCGGCT GGGGTAACAC ACAGTTCTAT GGCCAACAGG CTATGGTGCT CCAAGAGGCC 
CGGGTTCCCA TCATAAGCAA CGAAGTTTGC AACAGCCCCG ACTTCTACGG GAATCAGATC 
AAGCCCAAGA TGTTCTGTGC TGGCTATCCT GAGGGTGGCA TTGATGCGTG CCAGGGCGAC 
AGTGGAGGCC CCTTTGTGTG TGAAGACAGC ATCTCTGGGA CATCAAGGTG GCGGCTATGT 
GGCATTGTAA GCTGGGGTAC GGGCTGTGCT TTGGCCCGGA AGCCAGGAGT GTACACCAAA 
GTCACTGACT TCCGGGAGTG GATCTTCAAG GCCATAAAGA CTCACTCCGA AGCCAGTGGC 
ATGGTGACTC AGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin.
eLife4e08887(2015 Dec)
Brunati M,Perucca S,Han L,Cattaneo A,Consolato F,Andolfo A,Schaeffer C,Olinger E,Peng J,Santambrogio S,Perrier R,Li S,Bokhove M,Bachi A,Hummler E,Devuyst O,Wu Q,Jovine L,Rampoldi L

Serine protease hepsin regulates hepatocyte size and hemodynamic retention of tumor cells by hepatocyte growth factor signaling in mice.
Hepatology (Baltimore, Md.)56(5)1913-23(2012 Nov)
Hsu YC,Huang HP,Yu IS,Su KY,Lin SR,Lin WC,Wu HL,Shi GY,Tao MH,Kao CH,Wu YM,Martin PE,Lin SY,Yang PC,Lin SW

Pla2g12b and Hpn are genes identified by mouse ENU mutagenesis that affect HDL cholesterol.
PloS one7(8)e43139(2012)
Aljakna A,Choi S,Savage H,Hageman Blair R,Gu T,Svenson KL,Churchill GA,Hibbs M,Korstanje R

Thyroxine treatments do not correct inner ear defects in tmprss1 mutant mice.
Neuroreport21(13)897-901(2010 Sep)
Hanifa S,Scott HS,Crewther P,Guipponi M,Tan J

The structure of the extracellular region of human hepsin reveals a serine protease domain and a novel scavenger receptor cysteine-rich (SRCR) domain.
Structure (London, England : 1993)11(9)1123-31(2003 Sep)
Somoza JR,Ho JD,Luong C,Ghate M,Sprengeler PA,Mortara K,Shrader WD,Sperandio D,Chan H,McGrath ME,Katz BA

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Hpn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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