Hmgcl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hmgcl
Entrez Gene ID	15356
Full Name	3-hydroxy-3-methylglutaryl-Coenzyme A lyase
Synonyms	AW476067,HL
General protein information	Preferred Names 3-hydroxy-3-methylglutaryl-Coenzyme A lyase Names hydroxymethylglutaryl-CoA lyase, mitochondrial 3-hydroxy-3-methylglutarate-CoA lyase HMG-CoA lyase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 D3\|4 68.14 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006538563.3	XP_006538626.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1
XM_017319984.1	XP_017175473.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2
XM_006538564.3	XP_006538627.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2
NM_008254.3	NP_032280.2	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 1 precursor
NM_008254.2	NP_032280.2	hydroxymethylglutaryl-CoA lyase, mitochondrial precursor
XM_006538564.4	XP_006538627.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1
NM_001357529.1	NP_001344458.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 2
NM_001357530.1	NP_001344459.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 3

Pathways from BioSystems

Homologies

	HMGCL	XP_001104722.2
Canis lupus familiaris (dog)	HMGCL	XP_003638949.1
Gallus gallus (chicken)	HMGCL	NP_001185643.1
Drosophila melanogaster (fruit fly)	CG10399	NP_609089.1
Pan troglodytes (chimpanzee)	HMGCL	XP_001166273.1
Homo sapiens (human)	HMGCL	NP_000182.2
Bos taurus (cattle)	HMGCL	NP_001068600.1
Mus musculus (house mouse)	Hmgcl	NP_032280.2
Rattus norvegicus (Norway rat)	Hmgcl	NP_077362.1
Arabidopsis thaliana (thale cress)	AT2G26800	NP_850087.1
Xenopus tropicalis (tropical clawed frog)	LOC100494904	XP_002934605.1
Danio rerio (zebrafish)	hmgcl	NP_957509.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008717	XP_314824.4
Caenorhabditis elegans (roundworm)	Y71G12B.10	NP_490889.1
Oryza sativa (rice)	Os01g0269000	NP_001042696.1

Gene Ontology
Bibliography

Related articles in PubMed

A Class of Reactive Acyl-CoA Species Reveals the Non-enzymatic Origins of Protein Acylation.
Wagner GR, Bhatt DP, O'Connell TM, Thompson JW, Dubois LG, Backos DS, Yang H, Mitchell GA, Ilkayeva OR, Stevens RD, Grimsrud PA, Hirschey MD
Cell metabolism25(4)823-837.e8(2017 Apr)

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
Wu Y, Wei FY, Kawarada L, Suzuki T, Araki K, Komohara Y, Fujimura A, Kaitsuka T, Takeya M, Oike Y, Suzuki T, Tomizawa K
PLoS genetics12(9)e1006355(2016 Sep)

Nutritional stress exacerbates hepatic steatosis induced by deletion of the histidine nucleotide-binding (Hint2) mitochondrial protein.
Martin J, Balmer ML, Rajendran S, Maurhofer O, Dufour JF, St-Pierre MV
American journal of physiology. Gastrointestinal and liver physiology310(7)G497-509(2016 Apr)

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Gineste C, De Winter JM, Kohl C, Witt CC, Giannesini B, Brohm K, Le Fur Y, Gretz N, Vilmen C, Pecchi E, Jubeau M, Cozzone PJ, Stienen GJ, Granzier H, Labeit S, Ottenheijm CA, Bendahan D, Gondin J
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)

A liver-specific defect of Acyl-CoA degradation produces hyperammonemia, hypoglycemia and a distinct hepatic Acyl-CoA pattern.
Gauthier N, Wu JW, Wang SP, Allard P, Mamer OA, Sweetman L, Moser AB, Kratz L, Alvarez F, Robitaille Y, L?pine F, Mitchell GA
PloS one8(7)e60581(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hmgcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hmgcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu33998	NM_001357529.1 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu33998	XM_006538563.3 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu33999	NM_001357530.1 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu33999	XM_017319984.1 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu15412	NM_008254.3 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu33999	XM_006538564.4 Latest version!	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu15412	NM_008254.2	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu33999	XM_006538564.3	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hmgcl ( NM_001357529.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344458.1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu33998
Clone ID Related Accession (Same CDS sequence)	XM_006538563.3 , NM_001357529.1
Accession Version	NM_001357529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL672076.10 and AK132035.1. On Nov 4, 2017 this sequence version replaced XM_006538563.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.199733.1, SRR1660817.265170.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCACTT TGCCAAAGCA GGTGAAGATC GTGGAAGTCG GTCCCCGAGA TGGGCTGCAG 
AATGAAAAGA GTATTGTACC CACCCCAGTG AAGATCAGGC TGATCGACAT GCTTTCCGAA 
GCAGGGCTCC CCGTGATCGA GGCCACCAGC TTTGTTTCTC CCAAGTGGGT GCCACAGATG 
GCTGACCACT CTGACGTCTT GAAGGGCATT CAGAAGTTTC CCGGCATCAA CTACCCAGTC 
CTGACTCCAA ACATGAAAGG CTTTGAGGAA GCGGTAGCTG CGGGTGCCAA GGAAGTGAGC 
GTCTTCGGTG CTGTGTCTGA GCTCTTCACC CGGAAGAACG CGAACTGCTC TATAGAGGAG 
AGTTTCCAGC GCTTTGCTGG AGTCATGCAG GCGGCCCAGG CCGCCAGCAT CTCTGTGAGA 
GGGTATGTCT CCTGTGCCCT CGGATGCCCC TACGAGGGGA AGGTCTCCCC GGCTAAAGTT 
GCTGAGGTTG CCAAGAAGTT GTACTCGATG GGCTGCTATG AGATCTCCCT TGGGGACACC 
ATCGGCGTAG GTACCCCAGG ACTCATGAAA GACATGCTGA CTGCTGTCAT GCATGAAGTG 
CCTGTGACAG CATTGGCTGT CCACTGCCAT GATACCTATG GTCAAGCTCT GGCCAACACC 
TTGGTGGCCC TGCAGATGGG TGTGAGTGTT GTGGACTCCT CTGTGGCAGG ACTTGGAGGC 
TGTCCCTATG CAAAAGGGGC ATCAGGGAAC TTGGCTACTG AGGACCTGGT CTACATGCTG 
AATGGCTTAG GGATTCACAC GGGTGTGAAC CTCCAGAAAC TTCTAGAAGC TGGGGACTTC 
ATCTGTCAAG CCCTTAACAG AAAAACTAGT TCCAAAGTGG CACAGGCCAC CTGCAAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344458.1
CDS	1458..2360
Translation	MGTLPKQVKIVEVGPRDGLQNEKSIVPTPVKIRLIDMLSEAGLPVIEATSFVSPKWVPQMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQRFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL

Target ORF information:

RefSeq Version	NM_001357529.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357529.1

ORF Insert Sequence:

ATGGGCACTT TGCCAAAGCA GGTGAAGATC GTGGAAGTCG GTCCCCGAGA TGGGCTGCAG 
AATGAAAAGA GTATTGTACC CACCCCAGTG AAGATCAGGC TGATCGACAT GCTTTCCGAA 
GCAGGGCTCC CCGTGATCGA GGCCACCAGC TTTGTTTCTC CCAAGTGGGT GCCACAGATG 
GCTGACCACT CTGACGTCTT GAAGGGCATT CAGAAGTTTC CCGGCATCAA CTACCCAGTC 
CTGACTCCAA ACATGAAAGG CTTTGAGGAA GCGGTAGCTG CGGGTGCCAA GGAAGTGAGC 
GTCTTCGGTG CTGTGTCTGA GCTCTTCACC CGGAAGAACG CGAACTGCTC TATAGAGGAG 
AGTTTCCAGC GCTTTGCTGG AGTCATGCAG GCGGCCCAGG CCGCCAGCAT CTCTGTGAGA 
GGGTATGTCT CCTGTGCCCT CGGATGCCCC TACGAGGGGA AGGTCTCCCC GGCTAAAGTT 
GCTGAGGTTG CCAAGAAGTT GTACTCGATG GGCTGCTATG AGATCTCCCT TGGGGACACC 
ATCGGCGTAG GTACCCCAGG ACTCATGAAA GACATGCTGA CTGCTGTCAT GCATGAAGTG 
CCTGTGACAG CATTGGCTGT CCACTGCCAT GATACCTATG GTCAAGCTCT GGCCAACACC 
TTGGTGGCCC TGCAGATGGG TGTGAGTGTT GTGGACTCCT CTGTGGCAGG ACTTGGAGGC 
TGTCCCTATG CAAAAGGGGC ATCAGGGAAC TTGGCTACTG AGGACCTGGT CTACATGCTG 
AATGGCTTAG GGATTCACAC GGGTGTGAAC CTCCAGAAAC TTCTAGAAGC TGGGGACTTC 
ATCTGTCAAG CCCTTAACAG AAAAACTAGT TCCAAAGTGG CACAGGCCAC CTGCAAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcl ( XM_006538563.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538626.1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33998
Clone ID Related Accession (Same CDS sequence)	XM_006538563.3 , NM_001357529.1
Accession Version	XM_006538563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACTT TGCCAAAGCA GGTGAAGATC GTGGAAGTCG GTCCCCGAGA TGGGCTGCAG 
AATGAAAAGA GTATTGTACC CACCCCAGTG AAGATCAGGC TGATCGACAT GCTTTCCGAA 
GCAGGGCTCC CCGTGATCGA GGCCACCAGC TTTGTTTCTC CCAAGTGGGT GCCACAGATG 
GCTGACCACT CTGACGTCTT GAAGGGCATT CAGAAGTTTC CCGGCATCAA CTACCCAGTC 
CTGACTCCAA ACATGAAAGG CTTTGAGGAA GCGGTAGCTG CGGGTGCCAA GGAAGTGAGC 
GTCTTCGGTG CTGTGTCTGA GCTCTTCACC CGGAAGAACG CGAACTGCTC TATAGAGGAG 
AGTTTCCAGC GCTTTGCTGG AGTCATGCAG GCGGCCCAGG CCGCCAGCAT CTCTGTGAGA 
GGGTATGTCT CCTGTGCCCT CGGATGCCCC TACGAGGGGA AGGTCTCCCC GGCTAAAGTT 
GCTGAGGTTG CCAAGAAGTT GTACTCGATG GGCTGCTATG AGATCTCCCT TGGGGACACC 
ATCGGCGTAG GTACCCCAGG ACTCATGAAA GACATGCTGA CTGCTGTCAT GCATGAAGTG 
CCTGTGACAG CATTGGCTGT CCACTGCCAT GATACCTATG GTCAAGCTCT GGCCAACACC 
TTGGTGGCCC TGCAGATGGG TGTGAGTGTT GTGGACTCCT CTGTGGCAGG ACTTGGAGGC 
TGTCCCTATG CAAAAGGGGC ATCAGGGAAC TTGGCTACTG AGGACCTGGT CTACATGCTG 
AATGGCTTAG GGATTCACAC GGGTGTGAAC CTCCAGAAAC TTCTAGAAGC TGGGGACTTC 
ATCTGTCAAG CCCTTAACAG AAAAACTAGT TCCAAAGTGG CACAGGCCAC CTGCAAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538626.1
CDS	1304..2206
Translation	MGTLPKQVKIVEVGPRDGLQNEKSIVPTPVKIRLIDMLSEAGLPVIEATSFVSPKWVPQMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQRFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL

Target ORF information:

RefSeq Version	XM_006538563.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538563.3

ORF Insert Sequence:

ATGGGCACTT TGCCAAAGCA GGTGAAGATC GTGGAAGTCG GTCCCCGAGA TGGGCTGCAG 
AATGAAAAGA GTATTGTACC CACCCCAGTG AAGATCAGGC TGATCGACAT GCTTTCCGAA 
GCAGGGCTCC CCGTGATCGA GGCCACCAGC TTTGTTTCTC CCAAGTGGGT GCCACAGATG 
GCTGACCACT CTGACGTCTT GAAGGGCATT CAGAAGTTTC CCGGCATCAA CTACCCAGTC 
CTGACTCCAA ACATGAAAGG CTTTGAGGAA GCGGTAGCTG CGGGTGCCAA GGAAGTGAGC 
GTCTTCGGTG CTGTGTCTGA GCTCTTCACC CGGAAGAACG CGAACTGCTC TATAGAGGAG 
AGTTTCCAGC GCTTTGCTGG AGTCATGCAG GCGGCCCAGG CCGCCAGCAT CTCTGTGAGA 
GGGTATGTCT CCTGTGCCCT CGGATGCCCC TACGAGGGGA AGGTCTCCCC GGCTAAAGTT 
GCTGAGGTTG CCAAGAAGTT GTACTCGATG GGCTGCTATG AGATCTCCCT TGGGGACACC 
ATCGGCGTAG GTACCCCAGG ACTCATGAAA GACATGCTGA CTGCTGTCAT GCATGAAGTG 
CCTGTGACAG CATTGGCTGT CCACTGCCAT GATACCTATG GTCAAGCTCT GGCCAACACC 
TTGGTGGCCC TGCAGATGGG TGTGAGTGTT GTGGACTCCT CTGTGGCAGG ACTTGGAGGC 
TGTCCCTATG CAAAAGGGGC ATCAGGGAAC TTGGCTACTG AGGACCTGGT CTACATGCTG 
AATGGCTTAG GGATTCACAC GGGTGTGAAC CTCCAGAAAC TTCTAGAAGC TGGGGACTTC 
ATCTGTCAAG CCCTTAACAG AAAAACTAGT TCCAAAGTGG CACAGGCCAC CTGCAAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcl ( NM_001357530.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344459.1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu33999
Clone ID Related Accession (Same CDS sequence)	XM_017319984.1 , XM_006538564.3 , XM_006538564.4 , NM_001357530.1
Accession Version	NM_001357530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL672076.10 and AK132035.1. On Nov 4, 2017 this sequence version replaced XM_017319984.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.35406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAGG AGTGGCGCCC AGGGCCCCAC CAGAGAAGGT TCTTGATGGC TGACCACTCT 
GACGTCTTGA AGGGCATTCA GAAGTTTCCC GGCATCAACT ACCCAGTCCT GACTCCAAAC 
ATGAAAGGCT TTGAGGAAGC GGTAGCTGCG GGTGCCAAGG AAGTGAGCGT CTTCGGTGCT 
GTGTCTGAGC TCTTCACCCG GAAGAACGCG AACTGCTCTA TAGAGGAGAG TTTCCAGCGC 
TTTGCTGGAG TCATGCAGGC GGCCCAGGCC GCCAGCATCT CTGTGAGAGG GTATGTCTCC 
TGTGCCCTCG GATGCCCCTA CGAGGGGAAG GTCTCCCCGG CTAAAGTTGC TGAGGTTGCC 
AAGAAGTTGT ACTCGATGGG CTGCTATGAG ATCTCCCTTG GGGACACCAT CGGCGTAGGT 
ACCCCAGGAC TCATGAAAGA CATGCTGACT GCTGTCATGC ATGAAGTGCC TGTGACAGCA 
TTGGCTGTCC ACTGCCATGA TACCTATGGT CAAGCTCTGG CCAACACCTT GGTGGCCCTG 
CAGATGGGTG TGAGTGTTGT GGACTCCTCT GTGGCAGGAC TTGGAGGCTG TCCCTATGCA 
AAAGGGGCAT CAGGGAACTT GGCTACTGAG GACCTGGTCT ACATGCTGAA TGGCTTAGGG 
ATTCACACGG GTGTGAACCT CCAGAAACTT CTAGAAGCTG GGGACTTCAT CTGTCAAGCC 
CTTAACAGAA AAACTAGTTC CAAAGTGGCA CAGGCCACCT GCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344459.1
CDS	1735..2505
Translation	MQKEWRPGPHQRRFLMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQRFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL

Target ORF information:

RefSeq Version	NM_001357530.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357530.1

ORF Insert Sequence:

ATGCAGAAGG AGTGGCGCCC AGGGCCCCAC CAGAGAAGGT TCTTGATGGC TGACCACTCT 
GACGTCTTGA AGGGCATTCA GAAGTTTCCC GGCATCAACT ACCCAGTCCT GACTCCAAAC 
ATGAAAGGCT TTGAGGAAGC GGTAGCTGCG GGTGCCAAGG AAGTGAGCGT CTTCGGTGCT 
GTGTCTGAGC TCTTCACCCG GAAGAACGCG AACTGCTCTA TAGAGGAGAG TTTCCAGCGC 
TTTGCTGGAG TCATGCAGGC GGCCCAGGCC GCCAGCATCT CTGTGAGAGG GTATGTCTCC 
TGTGCCCTCG GATGCCCCTA CGAGGGGAAG GTCTCCCCGG CTAAAGTTGC TGAGGTTGCC 
AAGAAGTTGT ACTCGATGGG CTGCTATGAG ATCTCCCTTG GGGACACCAT CGGCGTAGGT 
ACCCCAGGAC TCATGAAAGA CATGCTGACT GCTGTCATGC ATGAAGTGCC TGTGACAGCA 
TTGGCTGTCC ACTGCCATGA TACCTATGGT CAAGCTCTGG CCAACACCTT GGTGGCCCTG 
CAGATGGGTG TGAGTGTTGT GGACTCCTCT GTGGCAGGAC TTGGAGGCTG TCCCTATGCA 
AAAGGGGCAT CAGGGAACTT GGCTACTGAG GACCTGGTCT ACATGCTGAA TGGCTTAGGG 
ATTCACACGG GTGTGAACCT CCAGAAACTT CTAGAAGCTG GGGACTTCAT CTGTCAAGCC 
CTTAACAGAA AAACTAGTTC CAAAGTGGCA CAGGCCACCT GCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcl ( XM_017319984.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175473.1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu33999
Clone ID Related Accession (Same CDS sequence)	XM_017319984.1 , XM_006538564.3 , XM_006538564.4 , NM_001357530.1
Accession Version	XM_017319984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG AGTGGCGCCC AGGGCCCCAC CAGAGAAGGT TCTTGATGGC TGACCACTCT 
GACGTCTTGA AGGGCATTCA GAAGTTTCCC GGCATCAACT ACCCAGTCCT GACTCCAAAC 
ATGAAAGGCT TTGAGGAAGC GGTAGCTGCG GGTGCCAAGG AAGTGAGCGT CTTCGGTGCT 
GTGTCTGAGC TCTTCACCCG GAAGAACGCG AACTGCTCTA TAGAGGAGAG TTTCCAGCGC 
TTTGCTGGAG TCATGCAGGC GGCCCAGGCC GCCAGCATCT CTGTGAGAGG GTATGTCTCC 
TGTGCCCTCG GATGCCCCTA CGAGGGGAAG GTCTCCCCGG CTAAAGTTGC TGAGGTTGCC 
AAGAAGTTGT ACTCGATGGG CTGCTATGAG ATCTCCCTTG GGGACACCAT CGGCGTAGGT 
ACCCCAGGAC TCATGAAAGA CATGCTGACT GCTGTCATGC ATGAAGTGCC TGTGACAGCA 
TTGGCTGTCC ACTGCCATGA TACCTATGGT CAAGCTCTGG CCAACACCTT GGTGGCCCTG 
CAGATGGGTG TGAGTGTTGT GGACTCCTCT GTGGCAGGAC TTGGAGGCTG TCCCTATGCA 
AAAGGGGCAT CAGGGAACTT GGCTACTGAG GACCTGGTCT ACATGCTGAA TGGCTTAGGG 
ATTCACACGG GTGTGAACCT CCAGAAACTT CTAGAAGCTG GGGACTTCAT CTGTCAAGCC 
CTTAACAGAA AAACTAGTTC CAAAGTGGCA CAGGCCACCT GCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175473.1
CDS	2557..3327
Translation	MQKEWRPGPHQRRFLMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQRFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL

Target ORF information:

RefSeq Version	XM_017319984.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319984.1

ORF Insert Sequence:

ATGCAGAAGG AGTGGCGCCC AGGGCCCCAC CAGAGAAGGT TCTTGATGGC TGACCACTCT 
GACGTCTTGA AGGGCATTCA GAAGTTTCCC GGCATCAACT ACCCAGTCCT GACTCCAAAC 
ATGAAAGGCT TTGAGGAAGC GGTAGCTGCG GGTGCCAAGG AAGTGAGCGT CTTCGGTGCT 
GTGTCTGAGC TCTTCACCCG GAAGAACGCG AACTGCTCTA TAGAGGAGAG TTTCCAGCGC 
TTTGCTGGAG TCATGCAGGC GGCCCAGGCC GCCAGCATCT CTGTGAGAGG GTATGTCTCC 
TGTGCCCTCG GATGCCCCTA CGAGGGGAAG GTCTCCCCGG CTAAAGTTGC TGAGGTTGCC 
AAGAAGTTGT ACTCGATGGG CTGCTATGAG ATCTCCCTTG GGGACACCAT CGGCGTAGGT 
ACCCCAGGAC TCATGAAAGA CATGCTGACT GCTGTCATGC ATGAAGTGCC TGTGACAGCA 
TTGGCTGTCC ACTGCCATGA TACCTATGGT CAAGCTCTGG CCAACACCTT GGTGGCCCTG 
CAGATGGGTG TGAGTGTTGT GGACTCCTCT GTGGCAGGAC TTGGAGGCTG TCCCTATGCA 
AAAGGGGCAT CAGGGAACTT GGCTACTGAG GACCTGGTCT ACATGCTGAA TGGCTTAGGG 
ATTCACACGG GTGTGAACCT CCAGAAACTT CTAGAAGCTG GGGACTTCAT CTGTCAAGCC 
CTTAACAGAA AAACTAGTTC CAAAGTGGCA CAGGCCACCT GCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcl ( XM_006538564.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538627.1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X2, mRNA.

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CloneID	OMu33999
Clone ID Related Accession (Same CDS sequence)	XM_017319984.1 , XM_006538564.3 , XM_006538564.4 , NM_001357530.1
Accession Version	XM_006538564.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG AGTGGCGCCC AGGGCCCCAC CAGAGAAGGT TCTTGATGGC TGACCACTCT 
GACGTCTTGA AGGGCATTCA GAAGTTTCCC GGCATCAACT ACCCAGTCCT GACTCCAAAC 
ATGAAAGGCT TTGAGGAAGC GGTAGCTGCG GGTGCCAAGG AAGTGAGCGT CTTCGGTGCT 
GTGTCTGAGC TCTTCACCCG GAAGAACGCG AACTGCTCTA TAGAGGAGAG TTTCCAGCGC 
TTTGCTGGAG TCATGCAGGC GGCCCAGGCC GCCAGCATCT CTGTGAGAGG GTATGTCTCC 
TGTGCCCTCG GATGCCCCTA CGAGGGGAAG GTCTCCCCGG CTAAAGTTGC TGAGGTTGCC 
AAGAAGTTGT ACTCGATGGG CTGCTATGAG ATCTCCCTTG GGGACACCAT CGGCGTAGGT 
ACCCCAGGAC TCATGAAAGA CATGCTGACT GCTGTCATGC ATGAAGTGCC TGTGACAGCA 
TTGGCTGTCC ACTGCCATGA TACCTATGGT CAAGCTCTGG CCAACACCTT GGTGGCCCTG 
CAGATGGGTG TGAGTGTTGT GGACTCCTCT GTGGCAGGAC TTGGAGGCTG TCCCTATGCA 
AAAGGGGCAT CAGGGAACTT GGCTACTGAG GACCTGGTCT ACATGCTGAA TGGCTTAGGG 
ATTCACACGG GTGTGAACCT CCAGAAACTT CTAGAAGCTG GGGACTTCAT CTGTCAAGCC 
CTTAACAGAA AAACTAGTTC CAAAGTGGCA CAGGCCACCT GCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538627.1
CDS	385..1155
Translation	MQKEWRPGPHQRRFLMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQRFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL

Target ORF information:

RefSeq Version	XM_006538564.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538564.3

ORF Insert Sequence:

ATGCAGAAGG AGTGGCGCCC AGGGCCCCAC CAGAGAAGGT TCTTGATGGC TGACCACTCT 
GACGTCTTGA AGGGCATTCA GAAGTTTCCC GGCATCAACT ACCCAGTCCT GACTCCAAAC 
ATGAAAGGCT TTGAGGAAGC GGTAGCTGCG GGTGCCAAGG AAGTGAGCGT CTTCGGTGCT 
GTGTCTGAGC TCTTCACCCG GAAGAACGCG AACTGCTCTA TAGAGGAGAG TTTCCAGCGC 
TTTGCTGGAG TCATGCAGGC GGCCCAGGCC GCCAGCATCT CTGTGAGAGG GTATGTCTCC 
TGTGCCCTCG GATGCCCCTA CGAGGGGAAG GTCTCCCCGG CTAAAGTTGC TGAGGTTGCC 
AAGAAGTTGT ACTCGATGGG CTGCTATGAG ATCTCCCTTG GGGACACCAT CGGCGTAGGT 
ACCCCAGGAC TCATGAAAGA CATGCTGACT GCTGTCATGC ATGAAGTGCC TGTGACAGCA 
TTGGCTGTCC ACTGCCATGA TACCTATGGT CAAGCTCTGG CCAACACCTT GGTGGCCCTG 
CAGATGGGTG TGAGTGTTGT GGACTCCTCT GTGGCAGGAC TTGGAGGCTG TCCCTATGCA 
AAAGGGGCAT CAGGGAACTT GGCTACTGAG GACCTGGTCT ACATGCTGAA TGGCTTAGGG 
ATTCACACGG GTGTGAACCT CCAGAAACTT CTAGAAGCTG GGGACTTCAT CTGTCAAGCC 
CTTAACAGAA AAACTAGTTC CAAAGTGGCA CAGGCCACCT GCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcl ( NM_008254.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032280.2 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant 1, mRNA.

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CloneID	OMu15412
Clone ID Related Accession (Same CDS sequence)	NM_008254.3 , NM_008254.2
Accession Version	NM_008254.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL672076.10, BB852743.1 and AK132035.1. On Nov 4, 2017 this sequence version replaced NM_008254.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132035.1, AK145251.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCAG TGAGGAAGGC TTTCCCGCGG AGGCTGGTGG GCTTGACGTC CCTCCGGGCT 
GTGAGCACCT CCTCCATGGG CACTTTGCCA AAGCAGGTGA AGATCGTGGA AGTCGGTCCC 
CGAGATGGGC TGCAGAATGA AAAGAGTATT GTACCCACCC CAGTGAAGAT CAGGCTGATC 
GACATGCTTT CCGAAGCAGG GCTCCCCGTG ATCGAGGCCA CCAGCTTTGT TTCTCCCAAG 
TGGGTGCCAC AGATGGCTGA CCACTCTGAC GTCTTGAAGG GCATTCAGAA GTTTCCCGGC 
ATCAACTACC CAGTCCTGAC TCCAAACATG AAAGGCTTTG AGGAAGCGGT AGCTGCGGGT 
GCCAAGGAAG TGAGCGTCTT CGGTGCTGTG TCTGAGCTCT TCACCCGGAA GAACGCGAAC 
TGCTCTATAG AGGAGAGTTT CCAGCGCTTT GCTGGAGTCA TGCAGGCGGC CCAGGCCGCC 
AGCATCTCTG TGAGAGGGTA TGTCTCCTGT GCCCTCGGAT GCCCCTACGA GGGGAAGGTC 
TCCCCGGCTA AAGTTGCTGA GGTTGCCAAG AAGTTGTACT CGATGGGCTG CTATGAGATC 
TCCCTTGGGG ACACCATCGG CGTAGGTACC CCAGGACTCA TGAAAGACAT GCTGACTGCT 
GTCATGCATG AAGTGCCTGT GACAGCATTG GCTGTCCACT GCCATGATAC CTATGGTCAA 
GCTCTGGCCA ACACCTTGGT GGCCCTGCAG ATGGGTGTGA GTGTTGTGGA CTCCTCTGTG 
GCAGGACTTG GAGGCTGTCC CTATGCAAAA GGGGCATCAG GGAACTTGGC TACTGAGGAC 
CTGGTCTACA TGCTGAATGG CTTAGGGATT CACACGGGTG TGAACCTCCA GAAACTTCTA 
GAAGCTGGGG ACTTCATCTG TCAAGCCCTT AACAGAAAAA CTAGTTCCAA AGTGGCACAG 
GCCACCTGCA AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032280.2
CDS	34..1011
Translation	MASVRKAFPRRLVGLTSLRAVSTSSMGTLPKQVKIVEVGPRDGLQNEKSIVPTPVKIRLIDMLSEAGLPVIEATSFVSPKWVPQMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQRFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL

Target ORF information:

RefSeq Version	NM_008254.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008254.3

ORF Insert Sequence:

ATGGCGTCAG TGAGGAAGGC TTTCCCGCGG AGGCTGGTGG GCTTGACGTC CCTCCGGGCT 
GTGAGCACCT CCTCCATGGG CACTTTGCCA AAGCAGGTGA AGATCGTGGA AGTCGGTCCC 
CGAGATGGGC TGCAGAATGA AAAGAGTATT GTACCCACCC CAGTGAAGAT CAGGCTGATC 
GACATGCTTT CCGAAGCAGG GCTCCCCGTG ATCGAGGCCA CCAGCTTTGT TTCTCCCAAG 
TGGGTGCCAC AGATGGCTGA CCACTCTGAC GTCTTGAAGG GCATTCAGAA GTTTCCCGGC 
ATCAACTACC CAGTCCTGAC TCCAAACATG AAAGGCTTTG AGGAAGCGGT AGCTGCGGGT 
GCCAAGGAAG TGAGCGTCTT CGGTGCTGTG TCTGAGCTCT TCACCCGGAA GAACGCGAAC 
TGCTCTATAG AGGAGAGTTT CCAGCGCTTT GCTGGAGTCA TGCAGGCGGC CCAGGCCGCC 
AGCATCTCTG TGAGAGGGTA TGTCTCCTGT GCCCTCGGAT GCCCCTACGA GGGGAAGGTC 
TCCCCGGCTA AAGTTGCTGA GGTTGCCAAG AAGTTGTACT CGATGGGCTG CTATGAGATC 
TCCCTTGGGG ACACCATCGG CGTAGGTACC CCAGGACTCA TGAAAGACAT GCTGACTGCT 
GTCATGCATG AAGTGCCTGT GACAGCATTG GCTGTCCACT GCCATGATAC CTATGGTCAA 
GCTCTGGCCA ACACCTTGGT GGCCCTGCAG ATGGGTGTGA GTGTTGTGGA CTCCTCTGTG 
GCAGGACTTG GAGGCTGTCC CTATGCAAAA GGGGCATCAG GGAACTTGGC TACTGAGGAC 
CTGGTCTACA TGCTGAATGG CTTAGGGATT CACACGGGTG TGAACCTCCA GAAACTTCTA 
GAAGCTGGGG ACTTCATCTG TCAAGCCCTT AACAGAAAAA CTAGTTCCAA AGTGGCACAG 
GCCACCTGCA AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcl ( NM_008254.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032280.2 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), mRNA.

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CloneID	OMu15412
Clone ID Related Accession (Same CDS sequence)	NM_008254.3 , NM_008254.2
Accession Version	NM_008254.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial precursor
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB852743.1, AK132035.1 and BX631310.1. On Apr 1, 2008 this sequence version replaced NM_008254.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145251.1, AK132035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCAG TGAGGAAGGC TTTCCCGCGG AGGCTGGTGG GCTTGACGTC CCTCCGGGCT 
GTGAGCACCT CCTCCATGGG CACTTTGCCA AAGCAGGTGA AGATCGTGGA AGTCGGTCCC 
CGAGATGGGC TGCAGAATGA AAAGAGTATT GTACCCACCC CAGTGAAGAT CAGGCTGATC 
GACATGCTTT CCGAAGCAGG GCTCCCCGTG ATCGAGGCCA CCAGCTTTGT TTCTCCCAAG 
TGGGTGCCAC AGATGGCTGA CCACTCTGAC GTCTTGAAGG GCATTCAGAA GTTTCCCGGC 
ATCAACTACC CAGTCCTGAC TCCAAACATG AAAGGCTTTG AGGAAGCGGT AGCTGCGGGT 
GCCAAGGAAG TGAGCGTCTT CGGTGCTGTG TCTGAGCTCT TCACCCGGAA GAACGCGAAC 
TGCTCTATAG AGGAGAGTTT CCAGCGCTTT GCTGGAGTCA TGCAGGCGGC CCAGGCCGCC 
AGCATCTCTG TGAGAGGGTA TGTCTCCTGT GCCCTCGGAT GCCCCTACGA GGGGAAGGTC 
TCCCCGGCTA AAGTTGCTGA GGTTGCCAAG AAGTTGTACT CGATGGGCTG CTATGAGATC 
TCCCTTGGGG ACACCATCGG CGTAGGTACC CCAGGACTCA TGAAAGACAT GCTGACTGCT 
GTCATGCATG AAGTGCCTGT GACAGCATTG GCTGTCCACT GCCATGATAC CTATGGTCAA 
GCTCTGGCCA ACACCTTGGT GGCCCTGCAG ATGGGTGTGA GTGTTGTGGA CTCCTCTGTG 
GCAGGACTTG GAGGCTGTCC CTATGCAAAA GGGGCATCAG GGAACTTGGC TACTGAGGAC 
CTGGTCTACA TGCTGAATGG CTTAGGGATT CACACGGGTG TGAACCTCCA GAAACTTCTA 
GAAGCTGGGG ACTTCATCTG TCAAGCCCTT AACAGAAAAA CTAGTTCCAA AGTGGCACAG 
GCCACCTGCA AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032280.2
CDS	29..1006
Translation	MASVRKAFPRRLVGLTSLRAVSTSSMGTLPKQVKIVEVGPRDGLQNEKSIVPTPVKIRLIDMLSEAGLPVIEATSFVSPKWVPQMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQRFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL

Target ORF information:

RefSeq Version	NM_008254.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008254.2

ORF Insert Sequence:

ATGGCGTCAG TGAGGAAGGC TTTCCCGCGG AGGCTGGTGG GCTTGACGTC CCTCCGGGCT 
GTGAGCACCT CCTCCATGGG CACTTTGCCA AAGCAGGTGA AGATCGTGGA AGTCGGTCCC 
CGAGATGGGC TGCAGAATGA AAAGAGTATT GTACCCACCC CAGTGAAGAT CAGGCTGATC 
GACATGCTTT CCGAAGCAGG GCTCCCCGTG ATCGAGGCCA CCAGCTTTGT TTCTCCCAAG 
TGGGTGCCAC AGATGGCTGA CCACTCTGAC GTCTTGAAGG GCATTCAGAA GTTTCCCGGC 
ATCAACTACC CAGTCCTGAC TCCAAACATG AAAGGCTTTG AGGAAGCGGT AGCTGCGGGT 
GCCAAGGAAG TGAGCGTCTT CGGTGCTGTG TCTGAGCTCT TCACCCGGAA GAACGCGAAC 
TGCTCTATAG AGGAGAGTTT CCAGCGCTTT GCTGGAGTCA TGCAGGCGGC CCAGGCCGCC 
AGCATCTCTG TGAGAGGGTA TGTCTCCTGT GCCCTCGGAT GCCCCTACGA GGGGAAGGTC 
TCCCCGGCTA AAGTTGCTGA GGTTGCCAAG AAGTTGTACT CGATGGGCTG CTATGAGATC 
TCCCTTGGGG ACACCATCGG CGTAGGTACC CCAGGACTCA TGAAAGACAT GCTGACTGCT 
GTCATGCATG AAGTGCCTGT GACAGCATTG GCTGTCCACT GCCATGATAC CTATGGTCAA 
GCTCTGGCCA ACACCTTGGT GGCCCTGCAG ATGGGTGTGA GTGTTGTGGA CTCCTCTGTG 
GCAGGACTTG GAGGCTGTCC CTATGCAAAA GGGGCATCAG GGAACTTGGC TACTGAGGAC 
CTGGTCTACA TGCTGAATGG CTTAGGGATT CACACGGGTG TGAACCTCCA GAAACTTCTA 
GAAGCTGGGG ACTTCATCTG TCAAGCCCTT AACAGAAAAA CTAGTTCCAA AGTGGCACAG 
GCCACCTGCA AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcl ( XM_006538564.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538627.1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33999
Clone ID Related Accession (Same CDS sequence)	XM_017319984.1 , XM_006538564.3 , XM_006538564.4 , NM_001357530.1
Accession Version	XM_006538564.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538564.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG AGTGGCGCCC AGGGCCCCAC CAGAGAAGGT TCTTGATGGC TGACCACTCT 
GACGTCTTGA AGGGCATTCA GAAGTTTCCC GGCATCAACT ACCCAGTCCT GACTCCAAAC 
ATGAAAGGCT TTGAGGAAGC GGTAGCTGCG GGTGCCAAGG AAGTGAGCGT CTTCGGTGCT 
GTGTCTGAGC TCTTCACCCG GAAGAACGCG AACTGCTCTA TAGAGGAGAG TTTCCAGCGC 
TTTGCTGGAG TCATGCAGGC GGCCCAGGCC GCCAGCATCT CTGTGAGAGG GTATGTCTCC 
TGTGCCCTCG GATGCCCCTA CGAGGGGAAG GTCTCCCCGG CTAAAGTTGC TGAGGTTGCC 
AAGAAGTTGT ACTCGATGGG CTGCTATGAG ATCTCCCTTG GGGACACCAT CGGCGTAGGT 
ACCCCAGGAC TCATGAAAGA CATGCTGACT GCTGTCATGC ATGAAGTGCC TGTGACAGCA 
TTGGCTGTCC ACTGCCATGA TACCTATGGT CAAGCTCTGG CCAACACCTT GGTGGCCCTG 
CAGATGGGTG TGAGTGTTGT GGACTCCTCT GTGGCAGGAC TTGGAGGCTG TCCCTATGCA 
AAAGGGGCAT CAGGGAACTT GGCTACTGAG GACCTGGTCT ACATGCTGAA TGGCTTAGGG 
ATTCACACGG GTGTGAACCT CCAGAAACTT CTAGAAGCTG GGGACTTCAT CTGTCAAGCC 
CTTAACAGAA AAACTAGTTC CAAAGTGGCA CAGGCCACCT GCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538627.1
CDS	386..1156
Translation	MQKEWRPGPHQRRFLMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQRFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL

Target ORF information:

RefSeq Version	XM_006538564.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538564.4

ORF Insert Sequence:

ATGCAGAAGG AGTGGCGCCC AGGGCCCCAC CAGAGAAGGT TCTTGATGGC TGACCACTCT 
GACGTCTTGA AGGGCATTCA GAAGTTTCCC GGCATCAACT ACCCAGTCCT GACTCCAAAC 
ATGAAAGGCT TTGAGGAAGC GGTAGCTGCG GGTGCCAAGG AAGTGAGCGT CTTCGGTGCT 
GTGTCTGAGC TCTTCACCCG GAAGAACGCG AACTGCTCTA TAGAGGAGAG TTTCCAGCGC 
TTTGCTGGAG TCATGCAGGC GGCCCAGGCC GCCAGCATCT CTGTGAGAGG GTATGTCTCC 
TGTGCCCTCG GATGCCCCTA CGAGGGGAAG GTCTCCCCGG CTAAAGTTGC TGAGGTTGCC 
AAGAAGTTGT ACTCGATGGG CTGCTATGAG ATCTCCCTTG GGGACACCAT CGGCGTAGGT 
ACCCCAGGAC TCATGAAAGA CATGCTGACT GCTGTCATGC ATGAAGTGCC TGTGACAGCA 
TTGGCTGTCC ACTGCCATGA TACCTATGGT CAAGCTCTGG CCAACACCTT GGTGGCCCTG 
CAGATGGGTG TGAGTGTTGT GGACTCCTCT GTGGCAGGAC TTGGAGGCTG TCCCTATGCA 
AAAGGGGCAT CAGGGAACTT GGCTACTGAG GACCTGGTCT ACATGCTGAA TGGCTTAGGG 
ATTCACACGG GTGTGAACCT CCAGAAACTT CTAGAAGCTG GGGACTTCAT CTGTCAAGCC 
CTTAACAGAA AAACTAGTTC CAAAGTGGCA CAGGCCACCT GCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Class of Reactive Acyl-CoA Species Reveals the Non-enzymatic Origins of Protein Acylation.
Cell metabolism25(4)823-837.e8(2017 Apr)
Wagner GR,Bhatt DP,O'Connell TM,Thompson JW,Dubois LG,Backos DS,Yang H,Mitchell GA,Ilkayeva OR,Stevens RD,Grimsrud PA,Hirschey MD

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
PLoS genetics12(9)e1006355(2016 Sep)
Wu Y,Wei FY,Kawarada L,Suzuki T,Araki K,Komohara Y,Fujimura A,Kaitsuka T,Takeya M,Oike Y,Suzuki T,Tomizawa K

Nutritional stress exacerbates hepatic steatosis induced by deletion of the histidine nucleotide-binding (Hint2) mitochondrial protein.
American journal of physiology. Gastrointestinal and liver physiology310(7)G497-509(2016 Apr)
Martin J,Balmer ML,Rajendran S,Maurhofer O,Dufour JF,St-Pierre MV

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)
Gineste C,De Winter JM,Kohl C,Witt CC,Giannesini B,Brohm K,Le Fur Y,Gretz N,Vilmen C,Pecchi E,Jubeau M,Cozzone PJ,Stienen GJ,Granzier H,Labeit S,Ottenheijm CA,Bendahan D,Gondin J

A liver-specific defect of Acyl-CoA degradation produces hyperammonemia, hypoglycemia and a distinct hepatic Acyl-CoA pattern.
PloS one8(7)e60581(2013)
Gauthier N,Wu JW,Wang SP,Allard P,Mamer OA,Sweetman L,Moser AB,Kratz L,Alvarez F,Robitaille Y,L?pine F,Mitchell GA

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Hmgcl cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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