Hlx cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hlx
Entrez Gene ID	15284
Full Name	H2.0-like homeobox
Synonyms	Hlx1
General protein information	Preferred Names H2.0-like homeobox Names H2.0-like homeobox protein H2.0-like homeo box 1 homeobox protein HLX1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H5\|1 88.97 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008250.2	NP_032276.1	H2.0-like homeobox protein

Pathways from BioSystems

Homologies

	HLX	XP_001101930.1
Canis lupus familiaris (dog)	HLX	XP_005640993.1
Mus musculus (house mouse)	Hlx	NP_032276.1
Homo sapiens (human)	HLX	NP_068777.1
Pan troglodytes (chimpanzee)	HLX	XP_525068.2
Rattus norvegicus (Norway rat)	Hlx	NP_001071142.1
Danio rerio (zebrafish)	hlx1	NP_997866.1
Bos taurus (cattle)	HLX	NP_001094567.1
Xenopus tropicalis (tropical clawed frog)	hlx	XP_002936060.1

Gene Ontology
Bibliography

Related articles in PubMed

HLX is a candidate gene for a pattern of anomalies associated with congenital diaphragmatic hernia, short bowel, and asplenia.
Farrell SA, Sodhi S, Marshall CR, Guerin A, Slavotinek A, Paton T, Chong K, Sirkin WL, Scherer SW, B?rub?-Simard FA, Pilon N
American journal of medical genetics. Part A173(11)3070-3074(2017 Nov)

Transcription factor Hlx controls a systematic switch from white to brown fat through Prdm16-mediated co-activation.
Huang L, Pan D, Chen Q, Zhu LJ, Ou J, Wabitsch M, Wang YX
Nature communications8(1)68(2017 07)

MBD2 regulates TH17 differentiation and experimental autoimmune encephalomyelitis by controlling the homeostasis of T-bet/Hlx axis.
Zhong J, Yu Q, Yang P, Rao X, He L, Fang J, Tu Y, Zhang Z, Lai Q, Zhang S, Kuczma M, Kraj P, Xu JF, Gong F, Zhou J, Wen L, Eizirik DL, Du J, Wang W, Wang CY
Journal of autoimmunity5395-104(2014 Sep)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

The H2.0-like homeobox transcription factor modulates yolk sac vascular remodeling in mouse embryos.
Prahst C, Kasaai B, Moraes F, Jahnsen ED, Larrivee B, Villegas D, Pardanaud L, Pibouin-Fragner L, Zhang F, Zaun HC, Eichmann A, Jones EA
Arteriosclerosis, thrombosis, and vascular biology34(7)1468-76(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Prdm16 interacts with the transcription factor Hlx, which is stabilized in response to beta3-adrenergic signaling, to increase thermogenic gene expression and mitochondrial biogenesis in subcutaneous WAT.
Title: Transcription factor Hlx controls a systematic switch from white to brown fat through Prdm16-mediated co-activation.

methyl-CpG-binding domain protein 2 (MBD2), an epigenetic regulator, controls autoimmunity and EAE through T-bet/Hlx.
Title: MBD2 regulates TH17 differentiation and experimental autoimmune encephalomyelitis by controlling the homeostasis of T-bet/Hlx axis.

Hlx regulates sprouting in vitro, but its role in sprouting is nonessential in vivo. HLX is regulated by shear stress and a subtle defect in vascular remodeling is present in knockout embryos.
Title: The H2.0-like homeobox transcription factor modulates yolk sac vascular remodeling in mouse embryos.

Results shown that over-expressed Hlx in DC2.4 up-regulated the transcription and expression of IFN-gamma, increased the expression of maturation makers including CD40, CD80, CD86, MHC-I and MHC-II
Title: Over-expression of Hlx homeobox gene in DC2.4 dendritic cell enhances its maturation and antigen presentation.

Transient over-expression of Hlx in DC2.4 promotes DC2.4 maturation and up-regulates IL-12, IL-10 and TGF-beta expression. H1x modified DC2.4 cells seem to function as regulatory dendritic cells
Title: [Functional study of transcription factor Hlx modified dendritic cell line DC2.4].

The following Hlx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hlx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01599	NM_008250.2 Latest version!	Mus musculus H2.0-like homeobox (Hlx), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hlx ( NM_008250.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032276.1 Mus musculus H2.0-like homeobox (Hlx), mRNA.

Price & Availability↑

CloneID	OMu01599
Clone ID Related Accession (Same CDS sequence)	NM_008250.2
Accession Version	NM_008250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	H2.0-like homeobox protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC064068.1, AK036435.1, BB664380.1 and BC050146.1. On Apr 29, 2009 this sequence version replaced NM_008250.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064068.1, AK036435.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCGCAG CCGGCCTGGC TCCCTTCTAT GCTTCCAACT TTAGTCTCTG GTCAGCCGCC 
TACTGCTCCT CGGCGGGCCC GGGCGGCTGC TCCTTCGCCC TGGACCCAGC CGCTGTCAAG 
AAACCCTCTT TCTGCATCGC GGACATCCTG CACGCCGGCG TCGGGGAGCC GGGGCCCGCT 
GCAGAGGGAC TGGTGGGGGC CTCGGCAGCC CTCACCGCAC ACTTGGGCTC CGTGCACCCG 
CACGCCTCCT TCCAAGCTGC CGCCAGATCC CCGCTCCGTC CCACCCCGGT GGTGGCGCCC 
TCAGAAGTCC CGGCTGGCTT CCCTCAGCGG CTGTCTCCGC TCTCTGCCGC CTTCCACCAG 
CATCTCCCGC AGCAATCACC AACGCAGCAA CAGCAACCGC AGCAGCAGCC CCCGCCTCCA 
CCCCGGGCGG TCTCCCTGCA GCCACCGACT TCGGGGACGC GGGTGGTCCC CCACCATAGC 
GGCTCCGCCC CTGCCCCCTC CAGCAAAGAC CTCAAATTTG GTATCGATCG AATTTTGTCT 
GCGGAATTTG ACCCAAAAGT TAAGGAAGGC AACACTCTGA GAGATCTCAC ATCGCTGCTA 
ACCGGTGGGC GGCCTGCAGG GGTGCATCTA GCTGGCCTGC AGCCTTCAGC GGGACAGTTC 
TTCGCATCTC TAGATCCCAT TAGCGAGGCG TCTGCCATCC TCAGCCCCTT AAGCTCCAAC 
CCAAGAAATT CTGTTCAGCA TCAATTCCAA GACACATTTC CAGGTCCCTA TGCTGTGCTC 
ACTAAGGACA CCATGCCACA GACATACAAG CGGAAGCGCT CGTGGTCCCG TGCTGTCTTT 
TCCAACCTGC AAAGAAAAGG CCTGGAGAAG AGGTTTGAGA TCCAGAAGTA CGTGACCAAG 
CCAGACCGAA AGCAGCTGGC GGCCATGCTG GGCCTCACGG ATGCACAGGT GAAGGTGTGG 
TTCCAGAACC GGAGGATGAA GTGGCGGCAC TCTAAGGAGG CGCAGGCGCA GAAGGACAAG 
GACAAGGAAG CGGGCGAGAA GCCTTCCGGC GGAGTCCCCG CCGAGGGTGA ACGGGAGGAG 
AGGAGCCCCA GCCGTTCTGA GGGCGAAGCC GAGAGCGAGA GCAGTGACTC GGAGTCCCTG 
GACATGGCCC CCAGCGACAC GGAACGGACT GAGGGAACGG AGAGGTCCCT GCATCAAACC 
ACGGTCATCA AGGCCTCAGC CGCGGGTGCC CTCATCACTG CCAGCAGCAG CACGAGTGGT 
AGCAGCTTCA GTTTCAGCAG CACCAGCAGC CTGGGTAGCG GCAACACCCA CGTGGGGAGC 
GCCAGCAGCC TTGGCGGCAA CTGCTCGGAG CTGCCTTCGG CTCACCAGCC CTCAGTCACC 
AGCAGTCCTC AAAGCCCGGA GATCGCCCAG GCCCCGCTTG CAGGTTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032276.1
CDS	353..1783
Translation	MFAAGLAPFYASNFSLWSAAYCSSAGPGGCSFALDPAAVKKPSFCIADILHAGVGEPGPAAEGLVGASAALTAHLGSVHPHASFQAAARSPLRPTPVVAPSEVPAGFPQRLSPLSAAFHQHLPQQSPTQQQQPQQQPPPPPRAVSLQPPTSGTRVVPHHSGSAPAPSSKDLKFGIDRILSAEFDPKVKEGNTLRDLTSLLTGGRPAGVHLAGLQPSAGQFFASLDPISEASAILSPLSSNPRNSVQHQFQDTFPGPYAVLTKDTMPQTYKRKRSWSRAVFSNLQRKGLEKRFEIQKYVTKPDRKQLAAMLGLTDAQVKVWFQNRRMKWRHSKEAQAQKDKDKEAGEKPSGGVPAEGEREERSPSRSEGEAESESSDSESLDMAPSDTERTEGTERSLHQTTVIKASAAGALITASSSTSGSSFSFSSTSSLGSGNTHVGSASSLGGNCSELPSAHQPSVTSSPQSPEIAQAPLAGL

Target ORF information:

RefSeq Version	NM_008250.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus H2.0-like homeobox (Hlx), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008250.2

ORF Insert Sequence:

ATGTTCGCAG CCGGCCTGGC TCCCTTCTAT GCTTCCAACT TTAGTCTCTG GTCAGCCGCC 
TACTGCTCCT CGGCGGGCCC GGGCGGCTGC TCCTTCGCCC TGGACCCAGC CGCTGTCAAG 
AAACCCTCTT TCTGCATCGC GGACATCCTG CACGCCGGCG TCGGGGAGCC GGGGCCCGCT 
GCAGAGGGAC TGGTGGGGGC CTCGGCAGCC CTCACCGCAC ACTTGGGCTC CGTGCACCCG 
CACGCCTCCT TCCAAGCTGC CGCCAGATCC CCGCTCCGTC CCACCCCGGT GGTGGCGCCC 
TCAGAAGTCC CGGCTGGCTT CCCTCAGCGG CTGTCTCCGC TCTCTGCCGC CTTCCACCAG 
CATCTCCCGC AGCAATCACC AACGCAGCAA CAGCAACCGC AGCAGCAGCC CCCGCCTCCA 
CCCCGGGCGG TCTCCCTGCA GCCACCGACT TCGGGGACGC GGGTGGTCCC CCACCATAGC 
GGCTCCGCCC CTGCCCCCTC CAGCAAAGAC CTCAAATTTG GTATCGATCG AATTTTGTCT 
GCGGAATTTG ACCCAAAAGT TAAGGAAGGC AACACTCTGA GAGATCTCAC ATCGCTGCTA 
ACCGGTGGGC GGCCTGCAGG GGTGCATCTA GCTGGCCTGC AGCCTTCAGC GGGACAGTTC 
TTCGCATCTC TAGATCCCAT TAGCGAGGCG TCTGCCATCC TCAGCCCCTT AAGCTCCAAC 
CCAAGAAATT CTGTTCAGCA TCAATTCCAA GACACATTTC CAGGTCCCTA TGCTGTGCTC 
ACTAAGGACA CCATGCCACA GACATACAAG CGGAAGCGCT CGTGGTCCCG TGCTGTCTTT 
TCCAACCTGC AAAGAAAAGG CCTGGAGAAG AGGTTTGAGA TCCAGAAGTA CGTGACCAAG 
CCAGACCGAA AGCAGCTGGC GGCCATGCTG GGCCTCACGG ATGCACAGGT GAAGGTGTGG 
TTCCAGAACC GGAGGATGAA GTGGCGGCAC TCTAAGGAGG CGCAGGCGCA GAAGGACAAG 
GACAAGGAAG CGGGCGAGAA GCCTTCCGGC GGAGTCCCCG CCGAGGGTGA ACGGGAGGAG 
AGGAGCCCCA GCCGTTCTGA GGGCGAAGCC GAGAGCGAGA GCAGTGACTC GGAGTCCCTG 
GACATGGCCC CCAGCGACAC GGAACGGACT GAGGGAACGG AGAGGTCCCT GCATCAAACC 
ACGGTCATCA AGGCCTCAGC CGCGGGTGCC CTCATCACTG CCAGCAGCAG CACGAGTGGT 
AGCAGCTTCA GTTTCAGCAG CACCAGCAGC CTGGGTAGCG GCAACACCCA CGTGGGGAGC 
GCCAGCAGCC TTGGCGGCAA CTGCTCGGAG CTGCCTTCGG CTCACCAGCC CTCAGTCACC 
AGCAGTCCTC AAAGCCCGGA GATCGCCCAG GCCCCGCTTG CAGGTTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HLX is a candidate gene for a pattern of anomalies associated with congenital diaphragmatic hernia, short bowel, and asplenia.
American journal of medical genetics. Part A173(11)3070-3074(2017 Nov)
Farrell SA,Sodhi S,Marshall CR,Guerin A,Slavotinek A,Paton T,Chong K,Sirkin WL,Scherer SW,B?rub?-Simard FA,Pilon N

Transcription factor Hlx controls a systematic switch from white to brown fat through Prdm16-mediated co-activation.
Nature communications8(1)68(2017 07)
Huang L,Pan D,Chen Q,Zhu LJ,Ou J,Wabitsch M,Wang YX

MBD2 regulates TH17 differentiation and experimental autoimmune encephalomyelitis by controlling the homeostasis of T-bet/Hlx axis.
Journal of autoimmunity5395-104(2014 Sep)
Zhong J,Yu Q,Yang P,Rao X,He L,Fang J,Tu Y,Zhang Z,Lai Q,Zhang S,Kuczma M,Kraj P,Xu JF,Gong F,Zhou J,Wen L,Eizirik DL,Du J,Wang W,Wang CY

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

The H2.0-like homeobox transcription factor modulates yolk sac vascular remodeling in mouse embryos.
Arteriosclerosis, thrombosis, and vascular biology34(7)1468-76(2014 Jul)
Prahst C,Kasaai B,Moraes F,Jahnsen ED,Larrivee B,Villegas D,Pardanaud L,Pibouin-Fragner L,Zhang F,Zaun HC,Eichmann A,Jones EA

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Hlx cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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