Hint1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hint1
Entrez Gene ID	15254
Full Name	histidine triad nucleotide binding protein 1
Synonyms	AA673479,Hint,Ipk1,PKCI-1,PRKCNH1
General protein information	Preferred Names histidine triad nucleotide binding protein 1 Names histidine triad nucleotide-binding protein 1 PKC inhibitor/ interacting protein adenosine 5'-monophosphoramidase protein kinase C inhibitor 1 protein kinase C-interacting protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_008248.3	NP_032274.1	histidine triad nucleotide-binding protein 1
NM_008248.2	NP_032274.1	histidine triad nucleotide-binding protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HINT1	XP_003310848.1
Drosophila melanogaster (fruit fly)	CG2862	NP_608711.3
Xenopus tropicalis (tropical clawed frog)	hint1	NP_001016165.1
Bos taurus (cattle)	HINT1	NP_787006.1
Danio rerio (zebrafish)	hint1	NP_001005593.1
	HINT1	XP_001099859.1
Mus musculus (house mouse)	Hint1	NP_032274.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006353	XP_316373.4
Caenorhabditis elegans (roundworm)	hint-1	NP_492056.1
Arabidopsis thaliana (thale cress)	HIT3	NP_567038.1
Oryza sativa (rice)	Os03g0322500	NP_001049967.1
Canis lupus familiaris (dog)	HINT1	XP_531895.2
Gallus gallus (chicken)	HINT1	NP_990020.1
Homo sapiens (human)	HINT1	NP_005331.1
Rattus norvegicus (Norway rat)	Hint1	NP_001103077.1

Gene Ontology
Bibliography

Related articles in PubMed

Social isolation induces schizophrenia-like behavior potentially associated with HINT1, NMDA receptor 1, and dopamine receptor 2.
Li BJ, Liu P, Chu Z, Shang Y, Huan MX, Dang YH, Gao CG
Neuroreport28(8)462-469(2017 May)

Increased PKC activity and altered GSK3?/NMDAR function drive behavior cycling in HINT1-deficient mice: bipolarity or opposing forces.
Garz?n-Ni?o J, Rodr?guez-Mu?oz M, Cort?s-Montero E, S?nchez-Bl?zquez P
Scientific reports743468(2017 Feb)

Hint1 gene deficiency enhances the supraspinal nociceptive sensitivity in mice.
Liu F, Ma J, Liu P, Chu Z, Lei G, Jia XD, Wang JB, Dang YH
Brain and behavior6(8)e00496(2016 08)

The ON:OFF switch, ?1R-HINT1 protein, controls GPCR-NMDA receptor cross-regulation: implications in neurological disorders.
Rodr?guez-Mu?oz M, Cort?s-Montero E, Pozo-Rodrig?lvarez A, S?nchez-Bl?zquez P, Garz?n-Ni?o J
Oncotarget6(34)35458-77(2015 Nov)

Systems genetic analysis of hippocampal neuroanatomy and spatial learning in mice.
Delprato A, Bonheur B, Alg?o MP, Rosay P, Lu L, Williams RW, Crusio WE
Genes, brain, and behavior14(8)591-606(2015 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The absence of Hint1 gene enhances supraspinal nociceptive sensitivity in mice, including thermal, mechanical and inflammatory hyperalgesia.
Title: Hint1 gene deficiency enhances the supraspinal nociceptive sensitivity in mice.

It was observed that HINT1 protein binds to GPCRs and NMDAR NR1 subunits in a calcium-independent manner, whereas sigma1R binding to these proteins increases in the presence of calcium.
Title: The ON:OFF switch, ?1R-HINT1 protein, controls GPCR-NMDA receptor cross-regulation: implications in neurological disorders.

Results suggest that Hint1 deficiency leads to an increased protein level of PKC gamma in the brain and a compromised activation response of PKC gamma upon stimulation
Title: Hint1 knockout results in a compromised activation of protein kinase C gamma in the brain.

HINT1 is involved in the behavioral abnormalities induced by social isolation and exerts distinct roles in different encephalic regions.
Title: HINT1 is involved in the behavioral abnormalities induced by social isolation rearing.

Activated mu-opioid receptors stimulate the production of nitric oxide (NO), and the redox zinc switch RGSZ2 converts this signal into free zinc ions that are required to recruit the redox sensor PKCgamma to HINT1 proteins.
Title: The ?1 receptor engages the redox-regulated HINT1 protein to bring opioid analgesia under NMDA receptor negative control.

The following Hint1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hint1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17777	NM_008248.3 Latest version!	Mus musculus histidine triad nucleotide binding protein 1 (Hint1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu17777	NM_008248.2	Mus musculus histidine triad nucleotide binding protein 1 (Hint1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hint1 ( NM_008248.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032274.1 Mus musculus histidine triad nucleotide binding protein 1 (Hint1), mRNA.

Price & Availability↑

CloneID	OMu17777
Clone ID Related Accession (Same CDS sequence)	NM_008248.3 , NM_008248.2
Accession Version	NM_008248.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	histidine triad nucleotide-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY261801.1, BC070415.1 and BE995722.1. On Feb 16, 2018 this sequence version replaced NM_008248.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BE995722.1, BC070415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGACG AGATTGCCAA GGCTCAAGTG GCCCAGCCCG GCGGCGACAC GATCTTCGGC 
AAGATCATCC GCAAAGAAAT CCCCGCCAAG ATCATCTTCG AGGACGACCG GTGTCTTGCT 
TTTCATGACA TTTCCCCTCA AGCACCAACA CACTTTCTGG TGATACCCAA GAAGCATATA 
TCCCAGATTT CTGTAGCAGA TGATGATGAT GAAAGTCTTC TAGGACATTT AATGATTGTT 
GGCAAGAAAT GTGCTGCAGA TCTGGGCCTG AAGCGCGGGT ACCGGATGGT GGTGAATGAA 
GGTGCAGACG GGGGACAGTC TGTCTATCAC ATTCACCTCC ATGTCCTTGG GGGTCGGCAG 
ATGAACTGGC CTCCTGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032274.1
CDS	108..488
Translation	MADEIAKAQVAQPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVADDDDESLLGHLMIVGKKCAADLGLKRGYRMVVNEGADGGQSVYHIHLHVLGGRQMNWPPG

Target ORF information:

RefSeq Version	NM_008248.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus histidine triad nucleotide binding protein 1 (Hint1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008248.3

ORF Insert Sequence:

ATGGCTGACG AGATTGCCAA GGCTCAAGTG GCCCAGCCCG GCGGCGACAC GATCTTCGGC 
AAGATCATCC GCAAAGAAAT CCCCGCCAAG ATCATCTTCG AGGACGACCG GTGTCTTGCT 
TTTCATGACA TTTCCCCTCA AGCACCAACA CACTTTCTGG TGATACCCAA GAAGCATATA 
TCCCAGATTT CTGTAGCAGA TGATGATGAT GAAAGTCTTC TAGGACATTT AATGATTGTT 
GGCAAGAAAT GTGCTGCAGA TCTGGGCCTG AAGCGCGGGT ACCGGATGGT GGTGAATGAA 
GGTGCAGACG GGGGACAGTC TGTCTATCAC ATTCACCTCC ATGTCCTTGG GGGTCGGCAG 
ATGAACTGGC CTCCTGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hint1 ( NM_008248.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032274.1 Mus musculus histidine triad nucleotide binding protein 1 (Hint1), mRNA.

Price & Availability↑

CloneID	OMu17777
Clone ID Related Accession (Same CDS sequence)	NM_008248.3 , NM_008248.2
Accession Version	NM_008248.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	histidine triad nucleotide-binding protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL954817.9. On Mar 21, 2007 this sequence version replaced NM_008248.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK002965.2, BC070415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGACG AGATTGCCAA GGCTCAAGTG GCCCAGCCCG GCGGCGACAC GATCTTCGGC 
AAGATCATCC GCAAAGAAAT CCCCGCCAAG ATCATCTTCG AGGACGACCG GTGTCTTGCT 
TTTCATGACA TTTCCCCTCA AGCACCAACA CACTTTCTGG TGATACCCAA GAAGCATATA 
TCCCAGATTT CTGTAGCAGA TGATGATGAT GAAAGTCTTC TAGGACATTT AATGATTGTT 
GGCAAGAAAT GTGCTGCAGA TCTGGGCCTG AAGCGCGGGT ACCGGATGGT GGTGAATGAA 
GGTGCAGACG GGGGACAGTC TGTCTATCAC ATTCACCTCC ATGTCCTTGG GGGTCGGCAG 
ATGAACTGGC CTCCTGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032274.1
CDS	53..433
Translation	MADEIAKAQVAQPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVADDDDESLLGHLMIVGKKCAADLGLKRGYRMVVNEGADGGQSVYHIHLHVLGGRQMNWPPG

Target ORF information:

RefSeq Version	NM_008248.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus histidine triad nucleotide binding protein 1 (Hint1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008248.2

ORF Insert Sequence:

ATGGCTGACG AGATTGCCAA GGCTCAAGTG GCCCAGCCCG GCGGCGACAC GATCTTCGGC 
AAGATCATCC GCAAAGAAAT CCCCGCCAAG ATCATCTTCG AGGACGACCG GTGTCTTGCT 
TTTCATGACA TTTCCCCTCA AGCACCAACA CACTTTCTGG TGATACCCAA GAAGCATATA 
TCCCAGATTT CTGTAGCAGA TGATGATGAT GAAAGTCTTC TAGGACATTT AATGATTGTT 
GGCAAGAAAT GTGCTGCAGA TCTGGGCCTG AAGCGCGGGT ACCGGATGGT GGTGAATGAA 
GGTGCAGACG GGGGACAGTC TGTCTATCAC ATTCACCTCC ATGTCCTTGG GGGTCGGCAG 
ATGAACTGGC CTCCTGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Social isolation induces schizophrenia-like behavior potentially associated with HINT1, NMDA receptor 1, and dopamine receptor 2.
Neuroreport28(8)462-469(2017 May)
Li BJ,Liu P,Chu Z,Shang Y,Huan MX,Dang YH,Gao CG

Increased PKC activity and altered GSK3?/NMDAR function drive behavior cycling in HINT1-deficient mice: bipolarity or opposing forces.
Scientific reports743468(2017 Feb)
Garz?n-Ni?o J,Rodr?guez-Mu?oz M,Cort?s-Montero E,S?nchez-Bl?zquez P

Hint1 gene deficiency enhances the supraspinal nociceptive sensitivity in mice.
Brain and behavior6(8)e00496(2016 08)
Liu F,Ma J,Liu P,Chu Z,Lei G,Jia XD,Wang JB,Dang YH

The ON:OFF switch, ?1R-HINT1 protein, controls GPCR-NMDA receptor cross-regulation: implications in neurological disorders.
Oncotarget6(34)35458-77(2015 Nov)
Rodr?guez-Mu?oz M,Cort?s-Montero E,Pozo-Rodrig?lvarez A,S?nchez-Bl?zquez P,Garz?n-Ni?o J

Systems genetic analysis of hippocampal neuroanatomy and spatial learning in mice.
Genes, brain, and behavior14(8)591-606(2015 11)
Delprato A,Bonheur B,Alg?o MP,Rosay P,Lu L,Williams RW,Crusio WE

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Hint1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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