Hic1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hic1
Entrez Gene ID	15248
Full Name	hypermethylated in cancer 1
Synonyms	AA408311,HIC-1
General protein information	Preferred Names hypermethylated in cancer 1 Names hypermethylated in cancer 1 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 45.76 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314294.1	XP_017169783.1	hypermethylated in cancer 1 protein isoform X1
XM_006532275.1	XP_006532338.1	hypermethylated in cancer 1 protein isoform X2
XM_017314294.2	XP_017169783.1	hypermethylated in cancer 1 protein isoform X1
NM_001098203.1	NP_001091673.1	hypermethylated in cancer 1 protein isoform 2
NM_010430.2	NP_034560.2	hypermethylated in cancer 1 protein isoform 1
XM_006532275.2	XP_006532338.1	hypermethylated in cancer 1 protein isoform X2

Pathways from BioSystems

Homologies

	LOC721284	XP_001117386.2
Mus musculus (house mouse)	Hic1	NP_034560.2
Gallus gallus (chicken)	HIC1	NP_990567.1
Xenopus tropicalis (tropical clawed frog)	hic1	XP_004911694.1
Canis lupus familiaris (dog)	HIC1	XP_548318.2
Danio rerio (zebrafish)	hic1	NP_001002314.1
Rattus norvegicus (Norway rat)	LOC100912068	XP_003750910.1
Homo sapiens (human)	HIC1	NP_001091672.1
Bos taurus (cattle)	HIC1	XP_003587448.2

Gene Ontology
Bibliography

Related articles in PubMed

The transcriptional repressor HIC1 regulates intestinal immune homeostasis.
Burrows K, Antignano F, Bramhall M, Chenery A, Scheer S, Korinek V, Underhill TM, Zaph C
Mucosal immunology10(6)1518-1528(2017 Nov)

HIC1 loss promotes prostate cancer metastasis by triggering epithelial-mesenchymal transition.
Hao M, Li Y, Wang J, Qin J, Wang Y, Ding Y, Jiang M, Sun X, Zu L, Chang K, Lin G, Du J, Korinek V, Ye DW, Wang J
The Journal of pathology242(4)409-420(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HIC1 loss promotes prostate cancer metastasis by triggering epithelial-mesenchymal transition.
Title: HIC1 loss promotes prostate cancer metastasis by triggering epithelial-mesenchymal transition.

identify HIC1 as the first transcription factor in mammals able to recruit PRC2 to some target promoters through its interaction with Polycomb-like proteins.
Title: Hypermethylated in cancer 1 (HIC1) recruits polycomb repressive complex 2 (PRC2) to a subset of its target genes through interaction with human polycomb-like (hPCL) proteins.

Data show that Hic1 expression is absent in polyps from DH mice, with concomitant increased expression of two transcriptional repression targets of Hic1, Sirt1 and Sox9.
Title: Loss of a single Hic1 allele accelerates polyp formation in Apc(?716) mice.

A potential tumor suppressor role for Hic1 in breast cancer through transcriptional repression of ephrin-A1.
Title: A potential tumor suppressor role for Hic1 in breast cancer through transcriptional repression of ephrin-A1.

For the Hic-1 gene, but not p16, the p53 gene might protect against aberrant methylation. The iNOS gene might not be involved in methylation of the Hic-1 gene, whereas the promoter region of p16 could be prone to methylation in MEFs lacking the iNOS gene.
Title: Lipopolysaccharide induces aberrant hypermethylation of Hic-1 in mouse embryonic fibroblasts lacking p53 gene.

The following Hic1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hic1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu45604	XM_006532275.2 Latest version!	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu77748	XM_017314294.2 Latest version!	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu13312	NM_001098203.1 Latest version!	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu13323	NM_010430.2 Latest version!	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OMu77748	XM_017314294.1	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu45604	XM_006532275.1	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hic1 ( XM_006532275.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532338.1 Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45604
Clone ID Related Accession (Same CDS sequence)	XM_006532275.1 , XM_006532275.2
Accession Version	XM_006532275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	hypermethylated in cancer 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGACA CGATGGAGGC GCCTGGCCAT TCGAGGCAGC TACTGCTGCA GCTCAACAAT 
CAGCGCACCA AGGGCTTCTT GTGCGACGTG ATCATCGTGG TGCAGAATGC CCTCTTCCGC 
GCGCACAAGA ACGTGCTGGC GGCCAGCAGC GCCTACCTCA AGTCCCTGGT GGTGCATGAC 
AACCTGCTAA ACCTGGACCA TGACATGGTG AGCCCGGCCG TGTTCCGCCT GGTGCTGGAC 
TTCATCTACA CCGGCCGCCT GACTGACAGT GTTGAGGCCG CAGCAGCAGC AGCGGTGGCC 
CCGGGCGCGG AGCCGAGCCT GGGCGCTGTG CTGGCTGCTG CCAGCTACCT GCAGATCCCT 
GACCTCGTGG CTCTGTGCAA GAAGCGCCTC AAACGCCACG GCAAGTACTG CCACCTGCGG 
GGAGGAGGCA GCGGCGGCGG CGGCTACGCT CCTTACGGGC GGCCCGGCCG GGGCTTGAGG 
GCTGCCACGC CCGTCATCCA GGCTTGCTAC TCGTCCCCGG CCGGGCCACC GCCGCCGCCT 
GCCGCCGAGC CGCCGTCGGG CCCCGATGCA GCCGTCAACA CCCACTGCGC TGAGCTATAT 
GCTTCAGGCC CGGGCCCAGC AGCCTCACTC TGCGCCCCGG AGCGTCGCTG CTCCCCGCTT 
TGCGGCCTGG ATCTGTCCAA GAAGAGCCCG CCAGGTTCCT CGGTCCCCGA GCGACCGCTT 
AGTGAGCGCG AACTGCCTCC ACGCCCGGAT AGCCCTCCCG GTGCGGGGCC CGCAGTCTAC 
AAGGAGCCAT CACTCGCCCT GCCCCCGCTG CCGCCTTTGC CCTTCCAAAA GCTGGAGGAG 
GCCGTACCGA CTCCAGACCC GTTTCGAGGA AGCGGTGGCA GTCCGGGACC CGAGCCCCCC 
GGCCGCCCCG ACGGCTCCAG CCTCCTCTAC CGCTGGATGA AGCACGAGCC AGGCCTGGGT 
AGTTATGGCG ATGAACTGGT CCGGGATCGA GGCTCCCCGG GTGAGCGCCT GGAGGAGCGC 
GGTGGGGATC CTGCCGCCTC ACCCGGGGGC CCCCCGCTGG GCCTGGTACC CCCGCCACGC 
TACCCCGGGA GCCTGGACGG GCCAGGCACA GGAGCAGACG GCGACGACTA TAAGAGCAGC 
AGCGAGGAGA CCGGTAGCAG CGAAGACCCC AGCCCACCCG GCGGCCACCT GGAGGGCTAC 
CCATGCCCGC ACTTGGCTTA TGGTGAGCCT GAGAGCTTTG GTGACAACCT GTACGTGTGC 
ATCCCATGTG GCAAAGGCTT CCCCAGCTCG GAACAGCTGA ATGCACACGT GGAGGCTCAC 
GTAGAGGAAG AAGAGGCGTT ATATGGCAGG GCAGAGGCTG CTGAGGTGGC TGCTGGGGCC 
GCCGGCCTTG GGCCCCCCTT TGGTGGCGGT GGGGACAAGG TCACTGGGGC GCCGGGCGGA 
CTAGGAGAGC TGCTGCGGCC ATACCGCTGC GCGTCCTGCG ACAAGAGCTA CAAGGACCCG 
GCCACGCTGA GGCAGCACGA GAAGACACAC TGGCTGACAA GGCCCTATCC GTGTACCATC 
TGCGGAAAGA AGTTCACGCA ACGCGGAACC ATGACACGCC ATATGCGCAG CCACTTGGGC 
CTGAAGCCCT TTGCGTGCGA CGCGTGCGGC ATGCGCTTCA CCCGCCAGTA TCGCCTCACG 
GAGCACATGC GCATCCACTC TGGAGAGAAG CCCTACGAGT GCCAGGTGTG CGGTGGCAAG 
TTTGCTCAAC AGCGCAACCT CATCAGCCAT ATGAAGATGC ACGCTGTAGG TGGCGCGGCC 
GGCGCGGCCG GGGCGCTGGC TGGCCTGGGG GGACTACCTG GCGTCCCTGG CCCTGACGGC 
AAGGGCAAGC TCGACTTCCC TGAGGGTGTC TTTGCTGTGG CCCGCCTCAC AGCTGAACAG 
CTGAGTCTGA AGCAGCAGGA CAAGGCAGCT GCTGCCGAGC TGCTGGCGCA GACCACGCAC 
TTCCTGCACG ACCCCAAGGT GGCGCTGGAG AGCCTCTACC CGCTGGCTAA ATTCACTGCT 
GAGCTAGGAC TCAGCCCAGA CAAGGCGGCA GAGGTGCTGA GCCAGGGTGC GCACTTGGCC 
GCAGGACCGG ACAGCCGAAC CATCGACCGT TTCTCTCCCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532338.1
CDS	45..2189
Translation	MLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLTDSVEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGSGGGGYAPYGRPGRGLRAATPVIQACYSSPAGPPPPPAAEPPSGPDAAVNTHCAELYASGPGPAASLCAPERRCSPLCGLDLSKKSPPGSSVPERPLSERELPPRPDSPPGAGPAVYKEPSLALPPLPPLPFQKLEEAVPTPDPFRGSGGSPGPEPPGRPDGSSLLYRWMKHEPGLGSYGDELVRDRGSPGERLEERGGDPAASPGGPPLGLVPPPRYPGSLDGPGTGADGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVTGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDSRTIDRFSPT

Target ORF information:

RefSeq Version	XM_006532275.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532275.2

ORF Insert Sequence:

ATGCTGGACA CGATGGAGGC GCCTGGCCAT TCGAGGCAGC TACTGCTGCA GCTCAACAAT 
CAGCGCACCA AGGGCTTCTT GTGCGACGTG ATCATCGTGG TGCAGAATGC CCTCTTCCGC 
GCGCACAAGA ACGTGCTGGC GGCCAGCAGC GCCTACCTCA AGTCCCTGGT GGTGCATGAC 
AACCTGCTAA ACCTGGACCA TGACATGGTG AGCCCGGCCG TGTTCCGCCT GGTGCTGGAC 
TTCATCTACA CCGGCCGCCT GACTGACAGT GTTGAGGCCG CAGCAGCAGC AGCGGTGGCC 
CCGGGCGCGG AGCCGAGCCT GGGCGCTGTG CTGGCTGCTG CCAGCTACCT GCAGATCCCT 
GACCTCGTGG CTCTGTGCAA GAAGCGCCTC AAACGCCACG GCAAGTACTG CCACCTGCGG 
GGAGGAGGCA GCGGCGGCGG CGGCTACGCT CCTTACGGGC GGCCCGGCCG GGGCTTGAGG 
GCTGCCACGC CCGTCATCCA GGCTTGCTAC TCGTCCCCGG CCGGGCCACC GCCGCCGCCT 
GCCGCCGAGC CGCCGTCGGG CCCCGATGCA GCCGTCAACA CCCACTGCGC TGAGCTATAT 
GCTTCAGGCC CGGGCCCAGC AGCCTCACTC TGCGCCCCGG AGCGTCGCTG CTCCCCGCTT 
TGCGGCCTGG ATCTGTCCAA GAAGAGCCCG CCAGGTTCCT CGGTCCCCGA GCGACCGCTT 
AGTGAGCGCG AACTGCCTCC ACGCCCGGAT AGCCCTCCCG GTGCGGGGCC CGCAGTCTAC 
AAGGAGCCAT CACTCGCCCT GCCCCCGCTG CCGCCTTTGC CCTTCCAAAA GCTGGAGGAG 
GCCGTACCGA CTCCAGACCC GTTTCGAGGA AGCGGTGGCA GTCCGGGACC CGAGCCCCCC 
GGCCGCCCCG ACGGCTCCAG CCTCCTCTAC CGCTGGATGA AGCACGAGCC AGGCCTGGGT 
AGTTATGGCG ATGAACTGGT CCGGGATCGA GGCTCCCCGG GTGAGCGCCT GGAGGAGCGC 
GGTGGGGATC CTGCCGCCTC ACCCGGGGGC CCCCCGCTGG GCCTGGTACC CCCGCCACGC 
TACCCCGGGA GCCTGGACGG GCCAGGCACA GGAGCAGACG GCGACGACTA TAAGAGCAGC 
AGCGAGGAGA CCGGTAGCAG CGAAGACCCC AGCCCACCCG GCGGCCACCT GGAGGGCTAC 
CCATGCCCGC ACTTGGCTTA TGGTGAGCCT GAGAGCTTTG GTGACAACCT GTACGTGTGC 
ATCCCATGTG GCAAAGGCTT CCCCAGCTCG GAACAGCTGA ATGCACACGT GGAGGCTCAC 
GTAGAGGAAG AAGAGGCGTT ATATGGCAGG GCAGAGGCTG CTGAGGTGGC TGCTGGGGCC 
GCCGGCCTTG GGCCCCCCTT TGGTGGCGGT GGGGACAAGG TCACTGGGGC GCCGGGCGGA 
CTAGGAGAGC TGCTGCGGCC ATACCGCTGC GCGTCCTGCG ACAAGAGCTA CAAGGACCCG 
GCCACGCTGA GGCAGCACGA GAAGACACAC TGGCTGACAA GGCCCTATCC GTGTACCATC 
TGCGGAAAGA AGTTCACGCA ACGCGGAACC ATGACACGCC ATATGCGCAG CCACTTGGGC 
CTGAAGCCCT TTGCGTGCGA CGCGTGCGGC ATGCGCTTCA CCCGCCAGTA TCGCCTCACG 
GAGCACATGC GCATCCACTC TGGAGAGAAG CCCTACGAGT GCCAGGTGTG CGGTGGCAAG 
TTTGCTCAAC AGCGCAACCT CATCAGCCAT ATGAAGATGC ACGCTGTAGG TGGCGCGGCC 
GGCGCGGCCG GGGCGCTGGC TGGCCTGGGG GGACTACCTG GCGTCCCTGG CCCTGACGGC 
AAGGGCAAGC TCGACTTCCC TGAGGGTGTC TTTGCTGTGG CCCGCCTCAC AGCTGAACAG 
CTGAGTCTGA AGCAGCAGGA CAAGGCAGCT GCTGCCGAGC TGCTGGCGCA GACCACGCAC 
TTCCTGCACG ACCCCAAGGT GGCGCTGGAG AGCCTCTACC CGCTGGCTAA ATTCACTGCT 
GAGCTAGGAC TCAGCCCAGA CAAGGCGGCA GAGGTGCTGA GCCAGGGTGC GCACTTGGCC 
GCAGGACCGG ACAGCCGAAC CATCGACCGT TTCTCTCCCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hic1 ( XM_017314294.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169783.1 Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77748
Clone ID Related Accession (Same CDS sequence)	XM_017314294.1 , XM_017314294.2
Accession Version	XM_017314294.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	hypermethylated in cancer 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAA GAATGCGCCA CCATCCCTGG CTTAAGAGTT CCTCATTTGA AGACAGAACC 
TTCCCCTCCC CCCCCCCGAA TTGGCGCGAT ATAACATATA AATATGTACT CGGGAGCACT 
CAGCACAAAA CCCCCAGGTT TATGGGACTC CCAGGTCCGA TATCTGAGTC TCCAGGTGGG 
CACATCTGGA AGCGCGCGAA CCTGCAAGAT CTGCTCAGCT CCGCATCTGC AAGCCTCTTG 
GCCCAGGTGT GCGCCAGAGG TCGCAGCCCA GCCGCACACA GTCCCCGCGT GGCCGCGAGG 
TGGCGCCATG GCCGCGGCAG CGTCTGCCGG TTCGGGCCGC TCCAGATAAG AGTGTGCGGA 
AAGCGCGGAG GGGCTGAGAC GCGACCAGGA CGCGGGGAGG ACGGACCGGC AAGACAGACC 
GACCGGGGGC CCGGCGGGCG GAGGGCAGCG CACTGCAGCC ACGTCCCCCC CTGGATCCGC 
CGGCAGCCGG GCCCGGGGCT TCCGACATGC CCCCCAGGAG AGTGTGCTGG GCAGACGATG 
CTGGACACGA TGGAGGCGCC TGGCCATTCG AGGCAGCTAC TGCTGCAGCT CAACAATCAG 
CGCACCAAGG GCTTCTTGTG CGACGTGATC ATCGTGGTGC AGAATGCCCT CTTCCGCGCG 
CACAAGAACG TGCTGGCGGC CAGCAGCGCC TACCTCAAGT CCCTGGTGGT GCATGACAAC 
CTGCTAAACC TGGACCATGA CATGGTGAGC CCGGCCGTGT TCCGCCTGGT GCTGGACTTC 
ATCTACACCG GCCGCCTGAC TGACAGTGTT GAGGCCGCAG CAGCAGCAGC GGTGGCCCCG 
GGCGCGGAGC CGAGCCTGGG CGCTGTGCTG GCTGCTGCCA GCTACCTGCA GATCCCTGAC 
CTCGTGGCTC TGTGCAAGAA GCGCCTCAAA CGCCACGGCA AGTACTGCCA CCTGCGGGGA 
GGAGGCAGCG GCGGCGGCGG CTACGCTCCT TACGGGCCAC CGCCGCCGCC TGCCGCCGAG 
CCGCCGTCGG GCCCCGATGC AGCCGTCAAC ACCCACTGCG CTGAGCTATA TGCTTCAGGC 
CCGGGCCCAG CAGCCTCACT CTGCGCCCCG GAGCGTCGCT GCTCCCCGCT TTGCGGCCTG 
GATCTGTCCA AGAAGAGCCC GCCAGGTTCC TCGGTCCCCG AGCGACCGCT TAGTGAGCGC 
GAACTGCCTC CACGCCCGGA TAGCCCTCCC GGTGCGGGGC CCGCAGTCTA CAAGGAGCCA 
TCACTCGCCC TGCCCCCGCT GCCGCCTTTG CCCTTCCAAA AGCTGGAGGA GGCCGTACCG 
ACTCCAGACC CGTTTCGAGG AAGCGGTGGC AGTCCGGGAC CCGAGCCCCC CGGCCGCCCC 
GACGGCTCCA GCCTCCTCTA CCGCTGGATG AAGCACGAGC CAGGCCTGGG TAGTTATGGC 
GATGAACTGG TCCGGGATCG AGGCTCCCCG GGTGAGCGCC TGGAGGAGCG CGGTGGGGAT 
CCTGCCGCCT CACCCGGGGG CCCCCCGCTG GGCCTGGTAC CCCCGCCACG CTACCCCGGG 
AGCCTGGACG GGCCAGGCAC AGGAGCAGAC GGCGACGACT ATAAGAGCAG CAGCGAGGAG 
ACCGGTAGCA GCGAAGACCC CAGCCCACCC GGCGGCCACC TGGAGGGCTA CCCATGCCCG 
CACTTGGCTT ATGGTGAGCC TGAGAGCTTT GGTGACAACC TGTACGTGTG CATCCCATGT 
GGCAAAGGCT TCCCCAGCTC GGAACAGCTG AATGCACACG TGGAGGCTCA CGTAGAGGAA 
GAAGAGGCGT TATATGGCAG GGCAGAGGCT GCTGAGGTGG CTGCTGGGGC CGCCGGCCTT 
GGGCCCCCCT TTGGTGGCGG TGGGGACAAG GTCACTGGGG CGCCGGGCGG ACTAGGAGAG 
CTGCTGCGGC CATACCGCTG CGCGTCCTGC GACAAGAGCT ACAAGGACCC GGCCACGCTG 
AGGCAGCACG AGAAGACACA CTGGCTGACA AGGCCCTATC CGTGTACCAT CTGCGGAAAG 
AAGTTCACGC AACGCGGAAC CATGACACGC CATATGCGCA GCCACTTGGG CCTGAAGCCC 
TTTGCGTGCG ACGCGTGCGG CATGCGCTTC ACCCGCCAGT ATCGCCTCAC GGAGCACATG 
CGCATCCACT CTGGAGAGAA GCCCTACGAG TGCCAGGTGT GCGGTGGCAA GTTTGCTCAA 
CAGCGCAACC TCATCAGCCA TATGAAGATG CACGCTGTAG GTGGCGCGGC CGGCGCGGCC 
GGGGCGCTGG CTGGCCTGGG GGGACTACCT GGCGTCCCTG GCCCTGACGG CAAGGGCAAG 
CTCGACTTCC CTGAGGGTGT CTTTGCTGTG GCCCGCCTCA CAGCTGAACA GCTGAGTCTG 
AAGCAGCAGG ACAAGGCAGC TGCTGCCGAG CTGCTGGCGC AGACCACGCA CTTCCTGCAC 
GACCCCAAGG TGGCGCTGGA GAGCCTCTAC CCGCTGGCTA AATTCACTGC TGAGCTAGGA 
CTCAGCCCAG ACAAGGCGGC AGAGGTGCTG AGCCAGGGTG CGCACTTGGC CGCAGGACCG 
GACAGCCGAA CCATCGACCG TTTCTCTCCC ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169783.1
CDS	732..3347
Translation	MITRMRHHPWLKSSSFEDRTFPSPPPNWRDITYKYVLGSTQHKTPRFMGLPGPISESPGGHIWKRANLQDLLSSASASLLAQVCARGRSPAAHSPRVAARWRHGRGSVCRFGPLQIRVCGKRGGAETRPGRGEDGPARQTDRGPGGRRAAHCSHVPPWIRRQPGPGLPTCPPGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLTDSVEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGSGGGGYAPYGPPPPPAAEPPSGPDAAVNTHCAELYASGPGPAASLCAPERRCSPLCGLDLSKKSPPGSSVPERPLSERELPPRPDSPPGAGPAVYKEPSLALPPLPPLPFQKLEEAVPTPDPFRGSGGSPGPEPPGRPDGSSLLYRWMKHEPGLGSYGDELVRDRGSPGERLEERGGDPAASPGGPPLGLVPPPRYPGSLDGPGTGADGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVTGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDSRTIDRFSPT

Target ORF information:

RefSeq Version	XM_017314294.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314294.2

ORF Insert Sequence:

ATGATTACAA GAATGCGCCA CCATCCCTGG CTTAAGAGTT CCTCATTTGA AGACAGAACC 
TTCCCCTCCC CCCCCCCGAA TTGGCGCGAT ATAACATATA AATATGTACT CGGGAGCACT 
CAGCACAAAA CCCCCAGGTT TATGGGACTC CCAGGTCCGA TATCTGAGTC TCCAGGTGGG 
CACATCTGGA AGCGCGCGAA CCTGCAAGAT CTGCTCAGCT CCGCATCTGC AAGCCTCTTG 
GCCCAGGTGT GCGCCAGAGG TCGCAGCCCA GCCGCACACA GTCCCCGCGT GGCCGCGAGG 
TGGCGCCATG GCCGCGGCAG CGTCTGCCGG TTCGGGCCGC TCCAGATAAG AGTGTGCGGA 
AAGCGCGGAG GGGCTGAGAC GCGACCAGGA CGCGGGGAGG ACGGACCGGC AAGACAGACC 
GACCGGGGGC CCGGCGGGCG GAGGGCAGCG CACTGCAGCC ACGTCCCCCC CTGGATCCGC 
CGGCAGCCGG GCCCGGGGCT TCCGACATGC CCCCCAGGAG AGTGTGCTGG GCAGACGATG 
CTGGACACGA TGGAGGCGCC TGGCCATTCG AGGCAGCTAC TGCTGCAGCT CAACAATCAG 
CGCACCAAGG GCTTCTTGTG CGACGTGATC ATCGTGGTGC AGAATGCCCT CTTCCGCGCG 
CACAAGAACG TGCTGGCGGC CAGCAGCGCC TACCTCAAGT CCCTGGTGGT GCATGACAAC 
CTGCTAAACC TGGACCATGA CATGGTGAGC CCGGCCGTGT TCCGCCTGGT GCTGGACTTC 
ATCTACACCG GCCGCCTGAC TGACAGTGTT GAGGCCGCAG CAGCAGCAGC GGTGGCCCCG 
GGCGCGGAGC CGAGCCTGGG CGCTGTGCTG GCTGCTGCCA GCTACCTGCA GATCCCTGAC 
CTCGTGGCTC TGTGCAAGAA GCGCCTCAAA CGCCACGGCA AGTACTGCCA CCTGCGGGGA 
GGAGGCAGCG GCGGCGGCGG CTACGCTCCT TACGGGCCAC CGCCGCCGCC TGCCGCCGAG 
CCGCCGTCGG GCCCCGATGC AGCCGTCAAC ACCCACTGCG CTGAGCTATA TGCTTCAGGC 
CCGGGCCCAG CAGCCTCACT CTGCGCCCCG GAGCGTCGCT GCTCCCCGCT TTGCGGCCTG 
GATCTGTCCA AGAAGAGCCC GCCAGGTTCC TCGGTCCCCG AGCGACCGCT TAGTGAGCGC 
GAACTGCCTC CACGCCCGGA TAGCCCTCCC GGTGCGGGGC CCGCAGTCTA CAAGGAGCCA 
TCACTCGCCC TGCCCCCGCT GCCGCCTTTG CCCTTCCAAA AGCTGGAGGA GGCCGTACCG 
ACTCCAGACC CGTTTCGAGG AAGCGGTGGC AGTCCGGGAC CCGAGCCCCC CGGCCGCCCC 
GACGGCTCCA GCCTCCTCTA CCGCTGGATG AAGCACGAGC CAGGCCTGGG TAGTTATGGC 
GATGAACTGG TCCGGGATCG AGGCTCCCCG GGTGAGCGCC TGGAGGAGCG CGGTGGGGAT 
CCTGCCGCCT CACCCGGGGG CCCCCCGCTG GGCCTGGTAC CCCCGCCACG CTACCCCGGG 
AGCCTGGACG GGCCAGGCAC AGGAGCAGAC GGCGACGACT ATAAGAGCAG CAGCGAGGAG 
ACCGGTAGCA GCGAAGACCC CAGCCCACCC GGCGGCCACC TGGAGGGCTA CCCATGCCCG 
CACTTGGCTT ATGGTGAGCC TGAGAGCTTT GGTGACAACC TGTACGTGTG CATCCCATGT 
GGCAAAGGCT TCCCCAGCTC GGAACAGCTG AATGCACACG TGGAGGCTCA CGTAGAGGAA 
GAAGAGGCGT TATATGGCAG GGCAGAGGCT GCTGAGGTGG CTGCTGGGGC CGCCGGCCTT 
GGGCCCCCCT TTGGTGGCGG TGGGGACAAG GTCACTGGGG CGCCGGGCGG ACTAGGAGAG 
CTGCTGCGGC CATACCGCTG CGCGTCCTGC GACAAGAGCT ACAAGGACCC GGCCACGCTG 
AGGCAGCACG AGAAGACACA CTGGCTGACA AGGCCCTATC CGTGTACCAT CTGCGGAAAG 
AAGTTCACGC AACGCGGAAC CATGACACGC CATATGCGCA GCCACTTGGG CCTGAAGCCC 
TTTGCGTGCG ACGCGTGCGG CATGCGCTTC ACCCGCCAGT ATCGCCTCAC GGAGCACATG 
CGCATCCACT CTGGAGAGAA GCCCTACGAG TGCCAGGTGT GCGGTGGCAA GTTTGCTCAA 
CAGCGCAACC TCATCAGCCA TATGAAGATG CACGCTGTAG GTGGCGCGGC CGGCGCGGCC 
GGGGCGCTGG CTGGCCTGGG GGGACTACCT GGCGTCCCTG GCCCTGACGG CAAGGGCAAG 
CTCGACTTCC CTGAGGGTGT CTTTGCTGTG GCCCGCCTCA CAGCTGAACA GCTGAGTCTG 
AAGCAGCAGG ACAAGGCAGC TGCTGCCGAG CTGCTGGCGC AGACCACGCA CTTCCTGCAC 
GACCCCAAGG TGGCGCTGGA GAGCCTCTAC CCGCTGGCTA AATTCACTGC TGAGCTAGGA 
CTCAGCCCAG ACAAGGCGGC AGAGGTGCTG AGCCAGGGTG CGCACTTGGC CGCAGGACCG 
GACAGCCGAA CCATCGACCG TTTCTCTCCC ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hic1 ( NM_001098203.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001091673.1 Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant 2, mRNA.

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CloneID	OMu13312
Clone ID Related Accession (Same CDS sequence)	NM_001098203.1
Accession Version	NM_001098203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	hypermethylated in cancer 1 protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ126522.1, AL603905.19 and BU757195.1. Transcript Variant: This variant (2) has an alternate 5' terminal exon including the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CJ126522.1, BF531899.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTTTC CTGAAGCGGA CATTTTACTT AAATCGGGAG AGTGTGCTGG GCAGACGATG 
CTGGACACGA TGGAGGCGCC TGGCCATTCG AGGCAGCTAC TGCTGCAGCT CAACAATCAG 
CGCACCAAGG GCTTCTTGTG CGACGTGATC ATCGTGGTGC AGAATGCCCT CTTCCGCGCG 
CACAAGAACG TGCTGGCGGC CAGCAGCGCC TACCTCAAGT CCCTGGTGGT GCATGACAAC 
CTGCTAAACC TGGACCATGA CATGGTGAGC CCGGCCGTGT TCCGCCTGGT GCTGGACTTC 
ATCTACACCG GCCGCCTGAC TGACAGTGTT GAGGCCGCAG CAGCAGCAGC GGTGGCCCCG 
GGCGCGGAGC CGAGCCTGGG CGCTGTGCTG GCTGCTGCCA GCTACCTGCA GATCCCTGAC 
CTCGTGGCTC TGTGCAAGAA GCGCCTCAAA CGCCACGGCA AGTACTGCCA CCTGCGGGGA 
GGAGGCAGCG GCGGCGGCGG CTACGCTCCT TACGGGCGGC CCGGCCGGGG CTTGAGGGCT 
GCCACGCCCG TCATCCAGGC TTGCTACTCG TCCCCGGCCG GGCCACCGCC GCCGCCTGCC 
GCCGAGCCGC CGTCGGGCCC CGATGCAGCC GTCAACACCC ACTGCGCTGA GCTATATGCT 
TCAGGCCCGG GCCCAGCAGC CTCACTCTGC GCCCCGGAGC GTCGCTGCTC CCCGCTTTGC 
GGCCTGGATC TGTCCAAGAA GAGCCCGCCA GGTTCCTCGG TCCCCGAGCG ACCGCTTAGT 
GAGCGCGAAC TGCCTCCACG CCCGGATAGC CCTCCCGGTG CGGGGCCCGC AGTCTACAAG 
GAGCCATCAC TCGCCCTGCC CCCGCTGCCG CCTTTGCCCT TCCAAAAGCT GGAGGAGGCC 
GTACCGACTC CAGACCCGTT TCGAGGAAGC GGTGGCAGTC CGGGACCCGA GCCCCCCGGC 
CGCCCCGACG GCTCCAGCCT CCTCTACCGC TGGATGAAGC ACGAGCCAGG CCTGGGTAGT 
TATGGCGATG AACTGGTCCG GGATCGAGGC TCCCCGGGTG AGCGCCTGGA GGAGCGCGGT 
GGGGATCCTG CCGCCTCACC CGGGGGCCCC CCGCTGGGCC TGGTACCCCC GCCACGCTAC 
CCCGGGAGCC TGGACGGGCC AGGCACAGGA GCAGACGGCG ACGACTATAA GAGCAGCAGC 
GAGGAGACCG GTAGCAGCGA AGACCCCAGC CCACCCGGCG GCCACCTGGA GGGCTACCCA 
TGCCCGCACT TGGCTTATGG TGAGCCTGAG AGCTTTGGTG ACAACCTGTA CGTGTGCATC 
CCATGTGGCA AAGGCTTCCC CAGCTCGGAA CAGCTGAATG CACACGTGGA GGCTCACGTA 
GAGGAAGAAG AGGCGTTATA TGGCAGGGCA GAGGCTGCTG AGGTGGCTGC TGGGGCCGCC 
GGCCTTGGGC CCCCCTTTGG TGGCGGTGGG GACAAGGTCA CTGGGGCGCC GGGCGGACTA 
GGAGAGCTGC TGCGGCCATA CCGCTGCGCG TCCTGCGACA AGAGCTACAA GGACCCGGCC 
ACGCTGAGGC AGCACGAGAA GACACACTGG CTGACAAGGC CCTATCCGTG TACCATCTGC 
GGAAAGAAGT TCACGCAACG CGGAACCATG ACACGCCATA TGCGCAGCCA CTTGGGCCTG 
AAGCCCTTTG CGTGCGACGC GTGCGGCATG CGCTTCACCC GCCAGTATCG CCTCACGGAG 
CACATGCGCA TCCACTCTGG AGAGAAGCCC TACGAGTGCC AGGTGTGCGG TGGCAAGTTT 
GCTCAACAGC GCAACCTCAT CAGCCATATG AAGATGCACG CTGTAGGTGG CGCGGCCGGC 
GCGGCCGGGG CGCTGGCTGG CCTGGGGGGA CTACCTGGCG TCCCTGGCCC TGACGGCAAG 
GGCAAGCTCG ACTTCCCTGA GGGTGTCTTT GCTGTGGCCC GCCTCACAGC TGAACAGCTG 
AGTCTGAAGC AGCAGGACAA GGCAGCTGCT GCCGAGCTGC TGGCGCAGAC CACGCACTTC 
CTGCACGACC CCAAGGTGGC GCTGGAGAGC CTCTACCCGC TGGCTAAATT CACTGCTGAG 
CTAGGACTCA GCCCAGACAA GGCGGCAGAG GTGCTGAGCC AGGGTGCGCA CTTGGCCGCA 
GGACCGGACA GCCGAACCAT CGACCGTTTC TCTCCCACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091673.1
CDS	223..2424
Translation	MTFPEADILLKSGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLTDSVEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGSGGGGYAPYGRPGRGLRAATPVIQACYSSPAGPPPPPAAEPPSGPDAAVNTHCAELYASGPGPAASLCAPERRCSPLCGLDLSKKSPPGSSVPERPLSERELPPRPDSPPGAGPAVYKEPSLALPPLPPLPFQKLEEAVPTPDPFRGSGGSPGPEPPGRPDGSSLLYRWMKHEPGLGSYGDELVRDRGSPGERLEERGGDPAASPGGPPLGLVPPPRYPGSLDGPGTGADGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVTGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDSRTIDRFSPT

Target ORF information:

RefSeq Version	NM_001098203.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098203.1

ORF Insert Sequence:

ATGACTTTTC CTGAAGCGGA CATTTTACTT AAATCGGGAG AGTGTGCTGG GCAGACGATG 
CTGGACACGA TGGAGGCGCC TGGCCATTCG AGGCAGCTAC TGCTGCAGCT CAACAATCAG 
CGCACCAAGG GCTTCTTGTG CGACGTGATC ATCGTGGTGC AGAATGCCCT CTTCCGCGCG 
CACAAGAACG TGCTGGCGGC CAGCAGCGCC TACCTCAAGT CCCTGGTGGT GCATGACAAC 
CTGCTAAACC TGGACCATGA CATGGTGAGC CCGGCCGTGT TCCGCCTGGT GCTGGACTTC 
ATCTACACCG GCCGCCTGAC TGACAGTGTT GAGGCCGCAG CAGCAGCAGC GGTGGCCCCG 
GGCGCGGAGC CGAGCCTGGG CGCTGTGCTG GCTGCTGCCA GCTACCTGCA GATCCCTGAC 
CTCGTGGCTC TGTGCAAGAA GCGCCTCAAA CGCCACGGCA AGTACTGCCA CCTGCGGGGA 
GGAGGCAGCG GCGGCGGCGG CTACGCTCCT TACGGGCGGC CCGGCCGGGG CTTGAGGGCT 
GCCACGCCCG TCATCCAGGC TTGCTACTCG TCCCCGGCCG GGCCACCGCC GCCGCCTGCC 
GCCGAGCCGC CGTCGGGCCC CGATGCAGCC GTCAACACCC ACTGCGCTGA GCTATATGCT 
TCAGGCCCGG GCCCAGCAGC CTCACTCTGC GCCCCGGAGC GTCGCTGCTC CCCGCTTTGC 
GGCCTGGATC TGTCCAAGAA GAGCCCGCCA GGTTCCTCGG TCCCCGAGCG ACCGCTTAGT 
GAGCGCGAAC TGCCTCCACG CCCGGATAGC CCTCCCGGTG CGGGGCCCGC AGTCTACAAG 
GAGCCATCAC TCGCCCTGCC CCCGCTGCCG CCTTTGCCCT TCCAAAAGCT GGAGGAGGCC 
GTACCGACTC CAGACCCGTT TCGAGGAAGC GGTGGCAGTC CGGGACCCGA GCCCCCCGGC 
CGCCCCGACG GCTCCAGCCT CCTCTACCGC TGGATGAAGC ACGAGCCAGG CCTGGGTAGT 
TATGGCGATG AACTGGTCCG GGATCGAGGC TCCCCGGGTG AGCGCCTGGA GGAGCGCGGT 
GGGGATCCTG CCGCCTCACC CGGGGGCCCC CCGCTGGGCC TGGTACCCCC GCCACGCTAC 
CCCGGGAGCC TGGACGGGCC AGGCACAGGA GCAGACGGCG ACGACTATAA GAGCAGCAGC 
GAGGAGACCG GTAGCAGCGA AGACCCCAGC CCACCCGGCG GCCACCTGGA GGGCTACCCA 
TGCCCGCACT TGGCTTATGG TGAGCCTGAG AGCTTTGGTG ACAACCTGTA CGTGTGCATC 
CCATGTGGCA AAGGCTTCCC CAGCTCGGAA CAGCTGAATG CACACGTGGA GGCTCACGTA 
GAGGAAGAAG AGGCGTTATA TGGCAGGGCA GAGGCTGCTG AGGTGGCTGC TGGGGCCGCC 
GGCCTTGGGC CCCCCTTTGG TGGCGGTGGG GACAAGGTCA CTGGGGCGCC GGGCGGACTA 
GGAGAGCTGC TGCGGCCATA CCGCTGCGCG TCCTGCGACA AGAGCTACAA GGACCCGGCC 
ACGCTGAGGC AGCACGAGAA GACACACTGG CTGACAAGGC CCTATCCGTG TACCATCTGC 
GGAAAGAAGT TCACGCAACG CGGAACCATG ACACGCCATA TGCGCAGCCA CTTGGGCCTG 
AAGCCCTTTG CGTGCGACGC GTGCGGCATG CGCTTCACCC GCCAGTATCG CCTCACGGAG 
CACATGCGCA TCCACTCTGG AGAGAAGCCC TACGAGTGCC AGGTGTGCGG TGGCAAGTTT 
GCTCAACAGC GCAACCTCAT CAGCCATATG AAGATGCACG CTGTAGGTGG CGCGGCCGGC 
GCGGCCGGGG CGCTGGCTGG CCTGGGGGGA CTACCTGGCG TCCCTGGCCC TGACGGCAAG 
GGCAAGCTCG ACTTCCCTGA GGGTGTCTTT GCTGTGGCCC GCCTCACAGC TGAACAGCTG 
AGTCTGAAGC AGCAGGACAA GGCAGCTGCT GCCGAGCTGC TGGCGCAGAC CACGCACTTC 
CTGCACGACC CCAAGGTGGC GCTGGAGAGC CTCTACCCGC TGGCTAAATT CACTGCTGAG 
CTAGGACTCA GCCCAGACAA GGCGGCAGAG GTGCTGAGCC AGGGTGCGCA CTTGGCCGCA 
GGACCGGACA GCCGAACCAT CGACCGTTTC TCTCCCACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hic1 ( NM_010430.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034560.2 Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant 1, mRNA.

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CloneID	OMu13323
Clone ID Related Accession (Same CDS sequence)	NM_010430.2
Accession Version	NM_010430.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	hypermethylated in cancer 1 protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL603905.19, CJ067923.1 and BU757195.1. On May 23, 2007 this sequence version replaced NM_010430.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ585343.1, AF036334.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTACAA GAATGCGCCA CCATCCCTGG CTTAAGAGTT CCTCATTTGA AGACAGAACC 
TTCCCCTCCC CCCCCCCGAA TTGGCGCGAT ATAACATATA AATATGTACT CGGGAGCACT 
CAGCACAAAA CCCCCAGGTT TATGGGACTC CCAGGTCCGA TATCTGAGTC TCCAGGTGGG 
CACATCTGGA AGCGCGCGAA CCTGCAAGAT CTGCTCAGCT CCGCATCTGC AAGCCTCTTG 
GCCCAGGTGT GCGCCAGAGG TCGCAGCCCA GCCGCACACA GTCCCCGCGT GGCCGCGAGG 
TGGCGCCATG GCCGCGGCAG CGTCTGCCGG TTCGGGCCGC TCCAGATAAG AGTGTGCGGA 
AAGCGCGGAG GGGCTGAGAC GCGACCAGGA CGCGGGGAGG ACGGACCGGC AAGACAGACC 
GACCGGGGGC CCGGCGGGCG GAGGGCAGCG CACTGCAGCC ACGTCCCCCC CTGGATCCGC 
CGGCAGCCGG GCCCGGGGCT TCCGACATGC CCCCCAGGAG AGTGTGCTGG GCAGACGATG 
CTGGACACGA TGGAGGCGCC TGGCCATTCG AGGCAGCTAC TGCTGCAGCT CAACAATCAG 
CGCACCAAGG GCTTCTTGTG CGACGTGATC ATCGTGGTGC AGAATGCCCT CTTCCGCGCG 
CACAAGAACG TGCTGGCGGC CAGCAGCGCC TACCTCAAGT CCCTGGTGGT GCATGACAAC 
CTGCTAAACC TGGACCATGA CATGGTGAGC CCGGCCGTGT TCCGCCTGGT GCTGGACTTC 
ATCTACACCG GCCGCCTGAC TGACAGTGTT GAGGCCGCAG CAGCAGCAGC GGTGGCCCCG 
GGCGCGGAGC CGAGCCTGGG CGCTGTGCTG GCTGCTGCCA GCTACCTGCA GATCCCTGAC 
CTCGTGGCTC TGTGCAAGAA GCGCCTCAAA CGCCACGGCA AGTACTGCCA CCTGCGGGGA 
GGAGGCAGCG GCGGCGGCGG CTACGCTCCT TACGGGCGGC CCGGCCGGGG CTTGAGGGCT 
GCCACGCCCG TCATCCAGGC TTGCTACTCG TCCCCGGCCG GGCCACCGCC GCCGCCTGCC 
GCCGAGCCGC CGTCGGGCCC CGATGCAGCC GTCAACACCC ACTGCGCTGA GCTATATGCT 
TCAGGCCCGG GCCCAGCAGC CTCACTCTGC GCCCCGGAGC GTCGCTGCTC CCCGCTTTGC 
GGCCTGGATC TGTCCAAGAA GAGCCCGCCA GGTTCCTCGG TCCCCGAGCG ACCGCTTAGT 
GAGCGCGAAC TGCCTCCACG CCCGGATAGC CCTCCCGGTG CGGGGCCCGC AGTCTACAAG 
GAGCCATCAC TCGCCCTGCC CCCGCTGCCG CCTTTGCCCT TCCAAAAGCT GGAGGAGGCC 
GTACCGACTC CAGACCCGTT TCGAGGAAGC GGTGGCAGTC CGGGACCCGA GCCCCCCGGC 
CGCCCCGACG GCTCCAGCCT CCTCTACCGC TGGATGAAGC ACGAGCCAGG CCTGGGTAGT 
TATGGCGATG AACTGGTCCG GGATCGAGGC TCCCCGGGTG AGCGCCTGGA GGAGCGCGGT 
GGGGATCCTG CCGCCTCACC CGGGGGCCCC CCGCTGGGCC TGGTACCCCC GCCACGCTAC 
CCCGGGAGCC TGGACGGGCC AGGCACAGGA GCAGACGGCG ACGACTATAA GAGCAGCAGC 
GAGGAGACCG GTAGCAGCGA AGACCCCAGC CCACCCGGCG GCCACCTGGA GGGCTACCCA 
TGCCCGCACT TGGCTTATGG TGAGCCTGAG AGCTTTGGTG ACAACCTGTA CGTGTGCATC 
CCATGTGGCA AAGGCTTCCC CAGCTCGGAA CAGCTGAATG CACACGTGGA GGCTCACGTA 
GAGGAAGAAG AGGCGTTATA TGGCAGGGCA GAGGCTGCTG AGGTGGCTGC TGGGGCCGCC 
GGCCTTGGGC CCCCCTTTGG TGGCGGTGGG GACAAGGTCA CTGGGGCGCC GGGCGGACTA 
GGAGAGCTGC TGCGGCCATA CCGCTGCGCG TCCTGCGACA AGAGCTACAA GGACCCGGCC 
ACGCTGAGGC AGCACGAGAA GACACACTGG CTGACAAGGC CCTATCCGTG TACCATCTGC 
GGAAAGAAGT TCACGCAACG CGGAACCATG ACACGCCATA TGCGCAGCCA CTTGGGCCTG 
AAGCCCTTTG CGTGCGACGC GTGCGGCATG CGCTTCACCC GCCAGTATCG CCTCACGGAG 
CACATGCGCA TCCACTCTGG AGAGAAGCCC TACGAGTGCC AGGTGTGCGG TGGCAAGTTT 
GCTCAACAGC GCAACCTCAT CAGCCATATG AAGATGCACG CTGTAGGTGG CGCGGCCGGC 
GCGGCCGGGG CGCTGGCTGG CCTGGGGGGA CTACCTGGCG TCCCTGGCCC TGACGGCAAG 
GGCAAGCTCG ACTTCCCTGA GGGTGTCTTT GCTGTGGCCC GCCTCACAGC TGAACAGCTG 
AGTCTGAAGC AGCAGGACAA GGCAGCTGCT GCCGAGCTGC TGGCGCAGAC CACGCACTTC 
CTGCACGACC CCAAGGTGGC GCTGGAGAGC CTCTACCCGC TGGCTAAATT CACTGCTGAG 
CTAGGACTCA GCCCAGACAA GGCGGCAGAG GTGCTGAGCC AGGGTGCGCA CTTGGCCGCA 
GGACCGGACA GCCGAACCAT CGACCGTTTC TCTCCCACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034560.2
CDS	737..3418
Translation	MITRMRHHPWLKSSSFEDRTFPSPPPNWRDITYKYVLGSTQHKTPRFMGLPGPISESPGGHIWKRANLQDLLSSASASLLAQVCARGRSPAAHSPRVAARWRHGRGSVCRFGPLQIRVCGKRGGAETRPGRGEDGPARQTDRGPGGRRAAHCSHVPPWIRRQPGPGLPTCPPGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLTDSVEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGSGGGGYAPYGRPGRGLRAATPVIQACYSSPAGPPPPPAAEPPSGPDAAVNTHCAELYASGPGPAASLCAPERRCSPLCGLDLSKKSPPGSSVPERPLSERELPPRPDSPPGAGPAVYKEPSLALPPLPPLPFQKLEEAVPTPDPFRGSGGSPGPEPPGRPDGSSLLYRWMKHEPGLGSYGDELVRDRGSPGERLEERGGDPAASPGGPPLGLVPPPRYPGSLDGPGTGADGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVTGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDSRTIDRFSPT

Target ORF information:

RefSeq Version	NM_010430.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010430.2

ORF Insert Sequence:

ATGATTACAA GAATGCGCCA CCATCCCTGG CTTAAGAGTT CCTCATTTGA AGACAGAACC 
TTCCCCTCCC CCCCCCCGAA TTGGCGCGAT ATAACATATA AATATGTACT CGGGAGCACT 
CAGCACAAAA CCCCCAGGTT TATGGGACTC CCAGGTCCGA TATCTGAGTC TCCAGGTGGG 
CACATCTGGA AGCGCGCGAA CCTGCAAGAT CTGCTCAGCT CCGCATCTGC AAGCCTCTTG 
GCCCAGGTGT GCGCCAGAGG TCGCAGCCCA GCCGCACACA GTCCCCGCGT GGCCGCGAGG 
TGGCGCCATG GCCGCGGCAG CGTCTGCCGG TTCGGGCCGC TCCAGATAAG AGTGTGCGGA 
AAGCGCGGAG GGGCTGAGAC GCGACCAGGA CGCGGGGAGG ACGGACCGGC AAGACAGACC 
GACCGGGGGC CCGGCGGGCG GAGGGCAGCG CACTGCAGCC ACGTCCCCCC CTGGATCCGC 
CGGCAGCCGG GCCCGGGGCT TCCGACATGC CCCCCAGGAG AGTGTGCTGG GCAGACGATG 
CTGGACACGA TGGAGGCGCC TGGCCATTCG AGGCAGCTAC TGCTGCAGCT CAACAATCAG 
CGCACCAAGG GCTTCTTGTG CGACGTGATC ATCGTGGTGC AGAATGCCCT CTTCCGCGCG 
CACAAGAACG TGCTGGCGGC CAGCAGCGCC TACCTCAAGT CCCTGGTGGT GCATGACAAC 
CTGCTAAACC TGGACCATGA CATGGTGAGC CCGGCCGTGT TCCGCCTGGT GCTGGACTTC 
ATCTACACCG GCCGCCTGAC TGACAGTGTT GAGGCCGCAG CAGCAGCAGC GGTGGCCCCG 
GGCGCGGAGC CGAGCCTGGG CGCTGTGCTG GCTGCTGCCA GCTACCTGCA GATCCCTGAC 
CTCGTGGCTC TGTGCAAGAA GCGCCTCAAA CGCCACGGCA AGTACTGCCA CCTGCGGGGA 
GGAGGCAGCG GCGGCGGCGG CTACGCTCCT TACGGGCGGC CCGGCCGGGG CTTGAGGGCT 
GCCACGCCCG TCATCCAGGC TTGCTACTCG TCCCCGGCCG GGCCACCGCC GCCGCCTGCC 
GCCGAGCCGC CGTCGGGCCC CGATGCAGCC GTCAACACCC ACTGCGCTGA GCTATATGCT 
TCAGGCCCGG GCCCAGCAGC CTCACTCTGC GCCCCGGAGC GTCGCTGCTC CCCGCTTTGC 
GGCCTGGATC TGTCCAAGAA GAGCCCGCCA GGTTCCTCGG TCCCCGAGCG ACCGCTTAGT 
GAGCGCGAAC TGCCTCCACG CCCGGATAGC CCTCCCGGTG CGGGGCCCGC AGTCTACAAG 
GAGCCATCAC TCGCCCTGCC CCCGCTGCCG CCTTTGCCCT TCCAAAAGCT GGAGGAGGCC 
GTACCGACTC CAGACCCGTT TCGAGGAAGC GGTGGCAGTC CGGGACCCGA GCCCCCCGGC 
CGCCCCGACG GCTCCAGCCT CCTCTACCGC TGGATGAAGC ACGAGCCAGG CCTGGGTAGT 
TATGGCGATG AACTGGTCCG GGATCGAGGC TCCCCGGGTG AGCGCCTGGA GGAGCGCGGT 
GGGGATCCTG CCGCCTCACC CGGGGGCCCC CCGCTGGGCC TGGTACCCCC GCCACGCTAC 
CCCGGGAGCC TGGACGGGCC AGGCACAGGA GCAGACGGCG ACGACTATAA GAGCAGCAGC 
GAGGAGACCG GTAGCAGCGA AGACCCCAGC CCACCCGGCG GCCACCTGGA GGGCTACCCA 
TGCCCGCACT TGGCTTATGG TGAGCCTGAG AGCTTTGGTG ACAACCTGTA CGTGTGCATC 
CCATGTGGCA AAGGCTTCCC CAGCTCGGAA CAGCTGAATG CACACGTGGA GGCTCACGTA 
GAGGAAGAAG AGGCGTTATA TGGCAGGGCA GAGGCTGCTG AGGTGGCTGC TGGGGCCGCC 
GGCCTTGGGC CCCCCTTTGG TGGCGGTGGG GACAAGGTCA CTGGGGCGCC GGGCGGACTA 
GGAGAGCTGC TGCGGCCATA CCGCTGCGCG TCCTGCGACA AGAGCTACAA GGACCCGGCC 
ACGCTGAGGC AGCACGAGAA GACACACTGG CTGACAAGGC CCTATCCGTG TACCATCTGC 
GGAAAGAAGT TCACGCAACG CGGAACCATG ACACGCCATA TGCGCAGCCA CTTGGGCCTG 
AAGCCCTTTG CGTGCGACGC GTGCGGCATG CGCTTCACCC GCCAGTATCG CCTCACGGAG 
CACATGCGCA TCCACTCTGG AGAGAAGCCC TACGAGTGCC AGGTGTGCGG TGGCAAGTTT 
GCTCAACAGC GCAACCTCAT CAGCCATATG AAGATGCACG CTGTAGGTGG CGCGGCCGGC 
GCGGCCGGGG CGCTGGCTGG CCTGGGGGGA CTACCTGGCG TCCCTGGCCC TGACGGCAAG 
GGCAAGCTCG ACTTCCCTGA GGGTGTCTTT GCTGTGGCCC GCCTCACAGC TGAACAGCTG 
AGTCTGAAGC AGCAGGACAA GGCAGCTGCT GCCGAGCTGC TGGCGCAGAC CACGCACTTC 
CTGCACGACC CCAAGGTGGC GCTGGAGAGC CTCTACCCGC TGGCTAAATT CACTGCTGAG 
CTAGGACTCA GCCCAGACAA GGCGGCAGAG GTGCTGAGCC AGGGTGCGCA CTTGGCCGCA 
GGACCGGACA GCCGAACCAT CGACCGTTTC TCTCCCACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hic1 ( XM_006532275.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532338.1 Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X2, mRNA.

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CloneID	OMu45604
Clone ID Related Accession (Same CDS sequence)	XM_006532275.1 , XM_006532275.2
Accession Version	XM_006532275.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	hypermethylated in cancer 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGACA CGATGGAGGC GCCTGGCCAT TCGAGGCAGC TACTGCTGCA GCTCAACAAT 
CAGCGCACCA AGGGCTTCTT GTGCGACGTG ATCATCGTGG TGCAGAATGC CCTCTTCCGC 
GCGCACAAGA ACGTGCTGGC GGCCAGCAGC GCCTACCTCA AGTCCCTGGT GGTGCATGAC 
AACCTGCTAA ACCTGGACCA TGACATGGTG AGCCCGGCCG TGTTCCGCCT GGTGCTGGAC 
TTCATCTACA CCGGCCGCCT GACTGACAGT GTTGAGGCCG CAGCAGCAGC AGCGGTGGCC 
CCGGGCGCGG AGCCGAGCCT GGGCGCTGTG CTGGCTGCTG CCAGCTACCT GCAGATCCCT 
GACCTCGTGG CTCTGTGCAA GAAGCGCCTC AAACGCCACG GCAAGTACTG CCACCTGCGG 
GGAGGAGGCA GCGGCGGCGG CGGCTACGCT CCTTACGGGC GGCCCGGCCG GGGCTTGAGG 
GCTGCCACGC CCGTCATCCA GGCTTGCTAC TCGTCCCCGG CCGGGCCACC GCCGCCGCCT 
GCCGCCGAGC CGCCGTCGGG CCCCGATGCA GCCGTCAACA CCCACTGCGC TGAGCTATAT 
GCTTCAGGCC CGGGCCCAGC AGCCTCACTC TGCGCCCCGG AGCGTCGCTG CTCCCCGCTT 
TGCGGCCTGG ATCTGTCCAA GAAGAGCCCG CCAGGTTCCT CGGTCCCCGA GCGACCGCTT 
AGTGAGCGCG AACTGCCTCC ACGCCCGGAT AGCCCTCCCG GTGCGGGGCC CGCAGTCTAC 
AAGGAGCCAT CACTCGCCCT GCCCCCGCTG CCGCCTTTGC CCTTCCAAAA GCTGGAGGAG 
GCCGTACCGA CTCCAGACCC GTTTCGAGGA AGCGGTGGCA GTCCGGGACC CGAGCCCCCC 
GGCCGCCCCG ACGGCTCCAG CCTCCTCTAC CGCTGGATGA AGCACGAGCC AGGCCTGGGT 
AGTTATGGCG ATGAACTGGT CCGGGATCGA GGCTCCCCGG GTGAGCGCCT GGAGGAGCGC 
GGTGGGGATC CTGCCGCCTC ACCCGGGGGC CCCCCGCTGG GCCTGGTACC CCCGCCACGC 
TACCCCGGGA GCCTGGACGG GCCAGGCACA GGAGCAGACG GCGACGACTA TAAGAGCAGC 
AGCGAGGAGA CCGGTAGCAG CGAAGACCCC AGCCCACCCG GCGGCCACCT GGAGGGCTAC 
CCATGCCCGC ACTTGGCTTA TGGTGAGCCT GAGAGCTTTG GTGACAACCT GTACGTGTGC 
ATCCCATGTG GCAAAGGCTT CCCCAGCTCG GAACAGCTGA ATGCACACGT GGAGGCTCAC 
GTAGAGGAAG AAGAGGCGTT ATATGGCAGG GCAGAGGCTG CTGAGGTGGC TGCTGGGGCC 
GCCGGCCTTG GGCCCCCCTT TGGTGGCGGT GGGGACAAGG TCACTGGGGC GCCGGGCGGA 
CTAGGAGAGC TGCTGCGGCC ATACCGCTGC GCGTCCTGCG ACAAGAGCTA CAAGGACCCG 
GCCACGCTGA GGCAGCACGA GAAGACACAC TGGCTGACAA GGCCCTATCC GTGTACCATC 
TGCGGAAAGA AGTTCACGCA ACGCGGAACC ATGACACGCC ATATGCGCAG CCACTTGGGC 
CTGAAGCCCT TTGCGTGCGA CGCGTGCGGC ATGCGCTTCA CCCGCCAGTA TCGCCTCACG 
GAGCACATGC GCATCCACTC TGGAGAGAAG CCCTACGAGT GCCAGGTGTG CGGTGGCAAG 
TTTGCTCAAC AGCGCAACCT CATCAGCCAT ATGAAGATGC ACGCTGTAGG TGGCGCGGCC 
GGCGCGGCCG GGGCGCTGGC TGGCCTGGGG GGACTACCTG GCGTCCCTGG CCCTGACGGC 
AAGGGCAAGC TCGACTTCCC TGAGGGTGTC TTTGCTGTGG CCCGCCTCAC AGCTGAACAG 
CTGAGTCTGA AGCAGCAGGA CAAGGCAGCT GCTGCCGAGC TGCTGGCGCA GACCACGCAC 
TTCCTGCACG ACCCCAAGGT GGCGCTGGAG AGCCTCTACC CGCTGGCTAA ATTCACTGCT 
GAGCTAGGAC TCAGCCCAGA CAAGGCGGCA GAGGTGCTGA GCCAGGGTGC GCACTTGGCC 
GCAGGACCGG ACAGCCGAAC CATCGACCGT TTCTCTCCCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532338.1
CDS	45..2189
Translation	MLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLTDSVEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGSGGGGYAPYGRPGRGLRAATPVIQACYSSPAGPPPPPAAEPPSGPDAAVNTHCAELYASGPGPAASLCAPERRCSPLCGLDLSKKSPPGSSVPERPLSERELPPRPDSPPGAGPAVYKEPSLALPPLPPLPFQKLEEAVPTPDPFRGSGGSPGPEPPGRPDGSSLLYRWMKHEPGLGSYGDELVRDRGSPGERLEERGGDPAASPGGPPLGLVPPPRYPGSLDGPGTGADGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVTGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDSRTIDRFSPT

Target ORF information:

RefSeq Version	XM_006532275.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532275.1

ORF Insert Sequence:

ATGCTGGACA CGATGGAGGC GCCTGGCCAT TCGAGGCAGC TACTGCTGCA GCTCAACAAT 
CAGCGCACCA AGGGCTTCTT GTGCGACGTG ATCATCGTGG TGCAGAATGC CCTCTTCCGC 
GCGCACAAGA ACGTGCTGGC GGCCAGCAGC GCCTACCTCA AGTCCCTGGT GGTGCATGAC 
AACCTGCTAA ACCTGGACCA TGACATGGTG AGCCCGGCCG TGTTCCGCCT GGTGCTGGAC 
TTCATCTACA CCGGCCGCCT GACTGACAGT GTTGAGGCCG CAGCAGCAGC AGCGGTGGCC 
CCGGGCGCGG AGCCGAGCCT GGGCGCTGTG CTGGCTGCTG CCAGCTACCT GCAGATCCCT 
GACCTCGTGG CTCTGTGCAA GAAGCGCCTC AAACGCCACG GCAAGTACTG CCACCTGCGG 
GGAGGAGGCA GCGGCGGCGG CGGCTACGCT CCTTACGGGC GGCCCGGCCG GGGCTTGAGG 
GCTGCCACGC CCGTCATCCA GGCTTGCTAC TCGTCCCCGG CCGGGCCACC GCCGCCGCCT 
GCCGCCGAGC CGCCGTCGGG CCCCGATGCA GCCGTCAACA CCCACTGCGC TGAGCTATAT 
GCTTCAGGCC CGGGCCCAGC AGCCTCACTC TGCGCCCCGG AGCGTCGCTG CTCCCCGCTT 
TGCGGCCTGG ATCTGTCCAA GAAGAGCCCG CCAGGTTCCT CGGTCCCCGA GCGACCGCTT 
AGTGAGCGCG AACTGCCTCC ACGCCCGGAT AGCCCTCCCG GTGCGGGGCC CGCAGTCTAC 
AAGGAGCCAT CACTCGCCCT GCCCCCGCTG CCGCCTTTGC CCTTCCAAAA GCTGGAGGAG 
GCCGTACCGA CTCCAGACCC GTTTCGAGGA AGCGGTGGCA GTCCGGGACC CGAGCCCCCC 
GGCCGCCCCG ACGGCTCCAG CCTCCTCTAC CGCTGGATGA AGCACGAGCC AGGCCTGGGT 
AGTTATGGCG ATGAACTGGT CCGGGATCGA GGCTCCCCGG GTGAGCGCCT GGAGGAGCGC 
GGTGGGGATC CTGCCGCCTC ACCCGGGGGC CCCCCGCTGG GCCTGGTACC CCCGCCACGC 
TACCCCGGGA GCCTGGACGG GCCAGGCACA GGAGCAGACG GCGACGACTA TAAGAGCAGC 
AGCGAGGAGA CCGGTAGCAG CGAAGACCCC AGCCCACCCG GCGGCCACCT GGAGGGCTAC 
CCATGCCCGC ACTTGGCTTA TGGTGAGCCT GAGAGCTTTG GTGACAACCT GTACGTGTGC 
ATCCCATGTG GCAAAGGCTT CCCCAGCTCG GAACAGCTGA ATGCACACGT GGAGGCTCAC 
GTAGAGGAAG AAGAGGCGTT ATATGGCAGG GCAGAGGCTG CTGAGGTGGC TGCTGGGGCC 
GCCGGCCTTG GGCCCCCCTT TGGTGGCGGT GGGGACAAGG TCACTGGGGC GCCGGGCGGA 
CTAGGAGAGC TGCTGCGGCC ATACCGCTGC GCGTCCTGCG ACAAGAGCTA CAAGGACCCG 
GCCACGCTGA GGCAGCACGA GAAGACACAC TGGCTGACAA GGCCCTATCC GTGTACCATC 
TGCGGAAAGA AGTTCACGCA ACGCGGAACC ATGACACGCC ATATGCGCAG CCACTTGGGC 
CTGAAGCCCT TTGCGTGCGA CGCGTGCGGC ATGCGCTTCA CCCGCCAGTA TCGCCTCACG 
GAGCACATGC GCATCCACTC TGGAGAGAAG CCCTACGAGT GCCAGGTGTG CGGTGGCAAG 
TTTGCTCAAC AGCGCAACCT CATCAGCCAT ATGAAGATGC ACGCTGTAGG TGGCGCGGCC 
GGCGCGGCCG GGGCGCTGGC TGGCCTGGGG GGACTACCTG GCGTCCCTGG CCCTGACGGC 
AAGGGCAAGC TCGACTTCCC TGAGGGTGTC TTTGCTGTGG CCCGCCTCAC AGCTGAACAG 
CTGAGTCTGA AGCAGCAGGA CAAGGCAGCT GCTGCCGAGC TGCTGGCGCA GACCACGCAC 
TTCCTGCACG ACCCCAAGGT GGCGCTGGAG AGCCTCTACC CGCTGGCTAA ATTCACTGCT 
GAGCTAGGAC TCAGCCCAGA CAAGGCGGCA GAGGTGCTGA GCCAGGGTGC GCACTTGGCC 
GCAGGACCGG ACAGCCGAAC CATCGACCGT TTCTCTCCCA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hic1 ( XM_017314294.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169783.1 Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X1, mRNA.

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CloneID	OMu77748
Clone ID Related Accession (Same CDS sequence)	XM_017314294.1 , XM_017314294.2
Accession Version	XM_017314294.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	hypermethylated in cancer 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAA GAATGCGCCA CCATCCCTGG CTTAAGAGTT CCTCATTTGA AGACAGAACC 
TTCCCCTCCC CCCCCCCGAA TTGGCGCGAT ATAACATATA AATATGTACT CGGGAGCACT 
CAGCACAAAA CCCCCAGGTT TATGGGACTC CCAGGTCCGA TATCTGAGTC TCCAGGTGGG 
CACATCTGGA AGCGCGCGAA CCTGCAAGAT CTGCTCAGCT CCGCATCTGC AAGCCTCTTG 
GCCCAGGTGT GCGCCAGAGG TCGCAGCCCA GCCGCACACA GTCCCCGCGT GGCCGCGAGG 
TGGCGCCATG GCCGCGGCAG CGTCTGCCGG TTCGGGCCGC TCCAGATAAG AGTGTGCGGA 
AAGCGCGGAG GGGCTGAGAC GCGACCAGGA CGCGGGGAGG ACGGACCGGC AAGACAGACC 
GACCGGGGGC CCGGCGGGCG GAGGGCAGCG CACTGCAGCC ACGTCCCCCC CTGGATCCGC 
CGGCAGCCGG GCCCGGGGCT TCCGACATGC CCCCCAGGAG AGTGTGCTGG GCAGACGATG 
CTGGACACGA TGGAGGCGCC TGGCCATTCG AGGCAGCTAC TGCTGCAGCT CAACAATCAG 
CGCACCAAGG GCTTCTTGTG CGACGTGATC ATCGTGGTGC AGAATGCCCT CTTCCGCGCG 
CACAAGAACG TGCTGGCGGC CAGCAGCGCC TACCTCAAGT CCCTGGTGGT GCATGACAAC 
CTGCTAAACC TGGACCATGA CATGGTGAGC CCGGCCGTGT TCCGCCTGGT GCTGGACTTC 
ATCTACACCG GCCGCCTGAC TGACAGTGTT GAGGCCGCAG CAGCAGCAGC GGTGGCCCCG 
GGCGCGGAGC CGAGCCTGGG CGCTGTGCTG GCTGCTGCCA GCTACCTGCA GATCCCTGAC 
CTCGTGGCTC TGTGCAAGAA GCGCCTCAAA CGCCACGGCA AGTACTGCCA CCTGCGGGGA 
GGAGGCAGCG GCGGCGGCGG CTACGCTCCT TACGGGCCAC CGCCGCCGCC TGCCGCCGAG 
CCGCCGTCGG GCCCCGATGC AGCCGTCAAC ACCCACTGCG CTGAGCTATA TGCTTCAGGC 
CCGGGCCCAG CAGCCTCACT CTGCGCCCCG GAGCGTCGCT GCTCCCCGCT TTGCGGCCTG 
GATCTGTCCA AGAAGAGCCC GCCAGGTTCC TCGGTCCCCG AGCGACCGCT TAGTGAGCGC 
GAACTGCCTC CACGCCCGGA TAGCCCTCCC GGTGCGGGGC CCGCAGTCTA CAAGGAGCCA 
TCACTCGCCC TGCCCCCGCT GCCGCCTTTG CCCTTCCAAA AGCTGGAGGA GGCCGTACCG 
ACTCCAGACC CGTTTCGAGG AAGCGGTGGC AGTCCGGGAC CCGAGCCCCC CGGCCGCCCC 
GACGGCTCCA GCCTCCTCTA CCGCTGGATG AAGCACGAGC CAGGCCTGGG TAGTTATGGC 
GATGAACTGG TCCGGGATCG AGGCTCCCCG GGTGAGCGCC TGGAGGAGCG CGGTGGGGAT 
CCTGCCGCCT CACCCGGGGG CCCCCCGCTG GGCCTGGTAC CCCCGCCACG CTACCCCGGG 
AGCCTGGACG GGCCAGGCAC AGGAGCAGAC GGCGACGACT ATAAGAGCAG CAGCGAGGAG 
ACCGGTAGCA GCGAAGACCC CAGCCCACCC GGCGGCCACC TGGAGGGCTA CCCATGCCCG 
CACTTGGCTT ATGGTGAGCC TGAGAGCTTT GGTGACAACC TGTACGTGTG CATCCCATGT 
GGCAAAGGCT TCCCCAGCTC GGAACAGCTG AATGCACACG TGGAGGCTCA CGTAGAGGAA 
GAAGAGGCGT TATATGGCAG GGCAGAGGCT GCTGAGGTGG CTGCTGGGGC CGCCGGCCTT 
GGGCCCCCCT TTGGTGGCGG TGGGGACAAG GTCACTGGGG CGCCGGGCGG ACTAGGAGAG 
CTGCTGCGGC CATACCGCTG CGCGTCCTGC GACAAGAGCT ACAAGGACCC GGCCACGCTG 
AGGCAGCACG AGAAGACACA CTGGCTGACA AGGCCCTATC CGTGTACCAT CTGCGGAAAG 
AAGTTCACGC AACGCGGAAC CATGACACGC CATATGCGCA GCCACTTGGG CCTGAAGCCC 
TTTGCGTGCG ACGCGTGCGG CATGCGCTTC ACCCGCCAGT ATCGCCTCAC GGAGCACATG 
CGCATCCACT CTGGAGAGAA GCCCTACGAG TGCCAGGTGT GCGGTGGCAA GTTTGCTCAA 
CAGCGCAACC TCATCAGCCA TATGAAGATG CACGCTGTAG GTGGCGCGGC CGGCGCGGCC 
GGGGCGCTGG CTGGCCTGGG GGGACTACCT GGCGTCCCTG GCCCTGACGG CAAGGGCAAG 
CTCGACTTCC CTGAGGGTGT CTTTGCTGTG GCCCGCCTCA CAGCTGAACA GCTGAGTCTG 
AAGCAGCAGG ACAAGGCAGC TGCTGCCGAG CTGCTGGCGC AGACCACGCA CTTCCTGCAC 
GACCCCAAGG TGGCGCTGGA GAGCCTCTAC CCGCTGGCTA AATTCACTGC TGAGCTAGGA 
CTCAGCCCAG ACAAGGCGGC AGAGGTGCTG AGCCAGGGTG CGCACTTGGC CGCAGGACCG 
GACAGCCGAA CCATCGACCG TTTCTCTCCC ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169783.1
CDS	732..3347
Translation	MITRMRHHPWLKSSSFEDRTFPSPPPNWRDITYKYVLGSTQHKTPRFMGLPGPISESPGGHIWKRANLQDLLSSASASLLAQVCARGRSPAAHSPRVAARWRHGRGSVCRFGPLQIRVCGKRGGAETRPGRGEDGPARQTDRGPGGRRAAHCSHVPPWIRRQPGPGLPTCPPGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLTDSVEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGSGGGGYAPYGPPPPPAAEPPSGPDAAVNTHCAELYASGPGPAASLCAPERRCSPLCGLDLSKKSPPGSSVPERPLSERELPPRPDSPPGAGPAVYKEPSLALPPLPPLPFQKLEEAVPTPDPFRGSGGSPGPEPPGRPDGSSLLYRWMKHEPGLGSYGDELVRDRGSPGERLEERGGDPAASPGGPPLGLVPPPRYPGSLDGPGTGADGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVTGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDSRTIDRFSPT

Target ORF information:

RefSeq Version	XM_017314294.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypermethylated in cancer 1 (Hic1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314294.1

ORF Insert Sequence:

ATGATTACAA GAATGCGCCA CCATCCCTGG CTTAAGAGTT CCTCATTTGA AGACAGAACC 
TTCCCCTCCC CCCCCCCGAA TTGGCGCGAT ATAACATATA AATATGTACT CGGGAGCACT 
CAGCACAAAA CCCCCAGGTT TATGGGACTC CCAGGTCCGA TATCTGAGTC TCCAGGTGGG 
CACATCTGGA AGCGCGCGAA CCTGCAAGAT CTGCTCAGCT CCGCATCTGC AAGCCTCTTG 
GCCCAGGTGT GCGCCAGAGG TCGCAGCCCA GCCGCACACA GTCCCCGCGT GGCCGCGAGG 
TGGCGCCATG GCCGCGGCAG CGTCTGCCGG TTCGGGCCGC TCCAGATAAG AGTGTGCGGA 
AAGCGCGGAG GGGCTGAGAC GCGACCAGGA CGCGGGGAGG ACGGACCGGC AAGACAGACC 
GACCGGGGGC CCGGCGGGCG GAGGGCAGCG CACTGCAGCC ACGTCCCCCC CTGGATCCGC 
CGGCAGCCGG GCCCGGGGCT TCCGACATGC CCCCCAGGAG AGTGTGCTGG GCAGACGATG 
CTGGACACGA TGGAGGCGCC TGGCCATTCG AGGCAGCTAC TGCTGCAGCT CAACAATCAG 
CGCACCAAGG GCTTCTTGTG CGACGTGATC ATCGTGGTGC AGAATGCCCT CTTCCGCGCG 
CACAAGAACG TGCTGGCGGC CAGCAGCGCC TACCTCAAGT CCCTGGTGGT GCATGACAAC 
CTGCTAAACC TGGACCATGA CATGGTGAGC CCGGCCGTGT TCCGCCTGGT GCTGGACTTC 
ATCTACACCG GCCGCCTGAC TGACAGTGTT GAGGCCGCAG CAGCAGCAGC GGTGGCCCCG 
GGCGCGGAGC CGAGCCTGGG CGCTGTGCTG GCTGCTGCCA GCTACCTGCA GATCCCTGAC 
CTCGTGGCTC TGTGCAAGAA GCGCCTCAAA CGCCACGGCA AGTACTGCCA CCTGCGGGGA 
GGAGGCAGCG GCGGCGGCGG CTACGCTCCT TACGGGCCAC CGCCGCCGCC TGCCGCCGAG 
CCGCCGTCGG GCCCCGATGC AGCCGTCAAC ACCCACTGCG CTGAGCTATA TGCTTCAGGC 
CCGGGCCCAG CAGCCTCACT CTGCGCCCCG GAGCGTCGCT GCTCCCCGCT TTGCGGCCTG 
GATCTGTCCA AGAAGAGCCC GCCAGGTTCC TCGGTCCCCG AGCGACCGCT TAGTGAGCGC 
GAACTGCCTC CACGCCCGGA TAGCCCTCCC GGTGCGGGGC CCGCAGTCTA CAAGGAGCCA 
TCACTCGCCC TGCCCCCGCT GCCGCCTTTG CCCTTCCAAA AGCTGGAGGA GGCCGTACCG 
ACTCCAGACC CGTTTCGAGG AAGCGGTGGC AGTCCGGGAC CCGAGCCCCC CGGCCGCCCC 
GACGGCTCCA GCCTCCTCTA CCGCTGGATG AAGCACGAGC CAGGCCTGGG TAGTTATGGC 
GATGAACTGG TCCGGGATCG AGGCTCCCCG GGTGAGCGCC TGGAGGAGCG CGGTGGGGAT 
CCTGCCGCCT CACCCGGGGG CCCCCCGCTG GGCCTGGTAC CCCCGCCACG CTACCCCGGG 
AGCCTGGACG GGCCAGGCAC AGGAGCAGAC GGCGACGACT ATAAGAGCAG CAGCGAGGAG 
ACCGGTAGCA GCGAAGACCC CAGCCCACCC GGCGGCCACC TGGAGGGCTA CCCATGCCCG 
CACTTGGCTT ATGGTGAGCC TGAGAGCTTT GGTGACAACC TGTACGTGTG CATCCCATGT 
GGCAAAGGCT TCCCCAGCTC GGAACAGCTG AATGCACACG TGGAGGCTCA CGTAGAGGAA 
GAAGAGGCGT TATATGGCAG GGCAGAGGCT GCTGAGGTGG CTGCTGGGGC CGCCGGCCTT 
GGGCCCCCCT TTGGTGGCGG TGGGGACAAG GTCACTGGGG CGCCGGGCGG ACTAGGAGAG 
CTGCTGCGGC CATACCGCTG CGCGTCCTGC GACAAGAGCT ACAAGGACCC GGCCACGCTG 
AGGCAGCACG AGAAGACACA CTGGCTGACA AGGCCCTATC CGTGTACCAT CTGCGGAAAG 
AAGTTCACGC AACGCGGAAC CATGACACGC CATATGCGCA GCCACTTGGG CCTGAAGCCC 
TTTGCGTGCG ACGCGTGCGG CATGCGCTTC ACCCGCCAGT ATCGCCTCAC GGAGCACATG 
CGCATCCACT CTGGAGAGAA GCCCTACGAG TGCCAGGTGT GCGGTGGCAA GTTTGCTCAA 
CAGCGCAACC TCATCAGCCA TATGAAGATG CACGCTGTAG GTGGCGCGGC CGGCGCGGCC 
GGGGCGCTGG CTGGCCTGGG GGGACTACCT GGCGTCCCTG GCCCTGACGG CAAGGGCAAG 
CTCGACTTCC CTGAGGGTGT CTTTGCTGTG GCCCGCCTCA CAGCTGAACA GCTGAGTCTG 
AAGCAGCAGG ACAAGGCAGC TGCTGCCGAG CTGCTGGCGC AGACCACGCA CTTCCTGCAC 
GACCCCAAGG TGGCGCTGGA GAGCCTCTAC CCGCTGGCTA AATTCACTGC TGAGCTAGGA 
CTCAGCCCAG ACAAGGCGGC AGAGGTGCTG AGCCAGGGTG CGCACTTGGC CGCAGGACCG 
GACAGCCGAA CCATCGACCG TTTCTCTCCC ACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The transcriptional repressor HIC1 regulates intestinal immune homeostasis.
Mucosal immunology10(6)1518-1528(2017 Nov)
Burrows K,Antignano F,Bramhall M,Chenery A,Scheer S,Korinek V,Underhill TM,Zaph C

HIC1 loss promotes prostate cancer metastasis by triggering epithelial-mesenchymal transition.
The Journal of pathology242(4)409-420(2017 Aug)
Hao M,Li Y,Wang J,Qin J,Wang Y,Ding Y,Jiang M,Sun X,Zu L,Chang K,Lin G,Du J,Korinek V,Ye DW,Wang J

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Hic1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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