Hells cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hells
Entrez Gene ID	15201
Full Name	helicase, lymphoid specific
Synonyms	AI323785,E130115I21Rik,LSH,Lysh,PASG,YFK8
General protein information	Preferred Names helicase, lymphoid specific Names lymphocyte-specific helicase hells proliferation-associated SNF2-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526700.3	XP_006526763.1	lymphocyte-specific helicase isoform X2
XM_006526699.4	XP_006526762.1	lymphocyte-specific helicase isoform X1
NM_008234.3	NP_032260.2	lymphocyte-specific helicase
XM_006526699.3	XP_006526762.1	lymphocyte-specific helicase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HELLS	XP_001151210.2
	HELLS	XP_001095601.1
Canis lupus familiaris (dog)	HELLS	XP_859353.2
Saccharomyces cerevisiae (baker's yeast)	IRC5	NP_116696.2
Kluyveromyces lactis	KLLA0E04159g	XP_454131.1
Mus musculus (house mouse)	Hells	NP_032260.2
Eremothecium gossypii	AGOS_ADL098C	NP_983998.1
Homo sapiens (human)	HELLS	NP_001275996.1
Bos taurus (cattle)	HELLS	XP_002698437.1

Gene Ontology
Bibliography

Related articles in PubMed

Tethering of Lsh at the Oct4 locus promotes gene repression associated with epigenetic changes.
Ren J, Hathaway NA, Crabtree GR, Muegge K
Epigenetics0(2017 Jun)

Lsh/HELLS regulates self-renewal/proliferation of neural stem/progenitor cells.
Han Y, Ren J, Lee E, Xu X, Yu W, Muegge K
Scientific reports7(1)1136(2017 Apr)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The SNF2 family ATPase LSH promotes cell-autonomous de novo DNA methylation in somatic cells.
Termanis A, Torrea N, Culley J, Kerr A, Ramsahoye B, Stancheva I
Nucleic acids research44(16)7592-604(2016 Sep)

Genome-wide DNA methylation patterns in LSH mutant reveals de-repression of repeat elements and redundant epigenetic silencing pathways.
Yu W, McIntosh C, Lister R, Zhu I, Han Y, Ren J, Landsman D, Lee E, Briones V, Terashima M, Leighty R, Ecker JR, Muegge K
Genome research24(10)1613-23(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

fibroblasts derived from chromatin remodeling ATPase LSH (HELLS)-null mouse embryos, which lack DNA methylation from centromeric repeats, transposons and a number of gene promoters, are capable of reestablishing DNA methylation and silencing of misregulated genes upon re-expression of LSH.
Title: The SNF2 family ATPase LSH promotes cell-autonomous de novo DNA methylation in somatic cells.

Data suggest that HELLS controls cytosine methylation in a nuclear compartment that is in part defined by lamin B1 attachment regions.
Title: Genome-wide DNA methylation patterns in LSH mutant reveals de-repression of repeat elements and redundant epigenetic silencing pathways.

histone H3 lysine 4 methylation modification was assessed in murine embryonic fibroblasts (MEFs) derived from the DNA methylation mutant Lsh(-/-) mice.
Title: CG hypomethylation in Lsh-/- mouse embryonic fibroblasts is associated with de novo H3K4me1 formation and altered cellular plasticity.

These results indicate that in the absence of HELLS, proliferation of spermatogonia is reduced and germ cell differentiation arrested at the midpachytene stage, implicating an essential role for HELLS during male meiosis.
Title: Lymphoid-specific helicase (HELLS) is essential for meiotic progression in mouse spermatocytes.

LSH is essential for developmentally programmed de novo DNA methylation at the promoters of protein coding genes and recruitment of G9a/GLP complex to a subset of genomic loci.
Title: LSH and G9a/GLP complex are required for developmentally programmed DNA methylation.

The following Hells gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hells cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu55264	XM_006526700.3 Latest version!	Mus musculus helicase, lymphoid specific (Hells), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu15031	NM_008234.3 Latest version!	Mus musculus helicase, lymphoid specific (Hells), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu15031	XM_006526699.4 Latest version!	Mus musculus helicase, lymphoid specific (Hells), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu15031	XM_006526699.3	Mus musculus helicase, lymphoid specific (Hells), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hells ( XM_006526700.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526763.1 Mus musculus helicase, lymphoid specific (Hells), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu55264
Clone ID Related Accession (Same CDS sequence)	XM_006526700.3
Accession Version	XM_006526700.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lymphocyte-specific helicase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526700.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTGCC GGCTAATCAG GGAGTTAAAA CGGTTCAATG CTGATAACAA ACTTCTTTTG 
ACTGGTACTC CCTTGCAAAA CAATTTATCA GAACTTTGGT CATTGCTAAA CTTTTTGTTG 
CCAGATGTAT TTGATGACTT GAAAAGCTTT GAGTCTTGGT TTGATATCAC TAGTCTTTCT 
GAAACTGCTG AAGATATTAT AGCTAAAGAG AGAGAACAGA ATGTTTTACA TATGCTGCAC 
CAGATTTTAA CGCCTTTCCT ACTGAGAAGA CTGAAGTCTG ATGTTGCTCT TGAAGTCCCC 
CCTAAGCGAG AAGTAGTTGT TTATGCACCA CTTTGCAATA AACAAGAGAT CTTCTATACA 
GCTATTGTGA ACCGCACAAT TGCAAACATG TTTGGATCCT GTGAGAAGGA AACAGTTGAG 
CTAAGTCCTA CTGGAAGACC AAAACGACGA AGTAGAAAGT CAATAAATTA CAGCGAACTA 
GATCAGTTCC CTAGTGAATT AGAAAAACTA ATAAGTCAAA TACAGCCAGA AGTAAACAGA 
GAGAGGACTG TTGTGGAAGG TAATATCCCT ATAGAATCTG AAGTTAATCT GAAGCTGCGG 
AATATAATGA TGCTACTTCG AAAGTGCTGT AATCACCCAT ATATGATTGA ATATCCTATT 
GACCCTGTCA CACAAGAATT TAAGATTGAT GAAGAGTTGG TAACAAATTC TGGGAAGTTC 
TTAATTTTGG ATCGAATGCT GCCCGAACTT AAAAAAAGAG GTCACAAGGT TCTGGTGTTT 
TCACAAATGA CAAGCATGTT GGACATCTTG ATGGATTATT GCCATCTTAG AAACTTCATC 
TTTAGCAGAC TTGATGGGTC CATGTCTTAT TCGGAAAGAG AAAAAAATAT ATACAGTTTC 
AACACGGATC CAGATGTTTT TCTTTTCTTA GTGAGTACAC GAGCTGGTGG TTTAGGCATA 
AACTTGACTG CAGCAGATAC AGTGATCATT TATGACAGTG ATTGGAATCC TCAGTCTGAT 
CTCCAGGCTC AAGATAGATG TCATAGAATT GGTCAGACAA AGCCAGTTGT TGTGTATCGT 
CTTGTAACAG CAAATACTAT TGACCAGAAA ATTGTGGAAA GAGCAGCTGC TAAAAGAAAG 
TTGGAGAAGT TGATAATCCA TAAAAATCAT TTCAAAGGTG GTCAGTCTGG GTTAAGTCAA 
TCTAAGAATT TCTTAGATGC AAAGGAATTA ATGGAGTTAT TAAAATCTAG AGATTATGAA 
AGGGAAGTAA AAGGATCCAG AGAGAAGGTC ATTAGTGACG AAGATCTAGA ATTACTGTTG 
GATCGAAGTG ATCTCATTGA TCAAATGAAG GCTTCAAGAC CAATTAAAGG GAAGACAGGG 
ATATTCAAAA TACTAGAAAA TTCTGAAGAT TCCAGTGCTG AATGTTTATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526763.1
CDS	196..1629
Translation	MKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSLSETAEDIIAKEREQNVLHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLCNKQEIFYTAIVNRTIANMFGSCEKETVELSPTGRPKRRSRKSINYSELDQFPSELEKLISQIQPEVNRERTVVEGNIPIESEVNLKLRNIMMLLRKCCNHPYMIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLVFSQMTSMLDILMDYCHLRNFIFSRLDGSMSYSEREKNIYSFNTDPDVFLFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLSQSKNFLDAKELMELLKSRDYEREVKGSREKVISDEDLELLLDRSDLIDQMKASRPIKGKTGIFKILENSEDSSAECLF

Target ORF information:

RefSeq Version	XM_006526700.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus helicase, lymphoid specific (Hells), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526700.3

ORF Insert Sequence:

ATGAAGTGCC GGCTAATCAG GGAGTTAAAA CGGTTCAATG CTGATAACAA ACTTCTTTTG 
ACTGGTACTC CCTTGCAAAA CAATTTATCA GAACTTTGGT CATTGCTAAA CTTTTTGTTG 
CCAGATGTAT TTGATGACTT GAAAAGCTTT GAGTCTTGGT TTGATATCAC TAGTCTTTCT 
GAAACTGCTG AAGATATTAT AGCTAAAGAG AGAGAACAGA ATGTTTTACA TATGCTGCAC 
CAGATTTTAA CGCCTTTCCT ACTGAGAAGA CTGAAGTCTG ATGTTGCTCT TGAAGTCCCC 
CCTAAGCGAG AAGTAGTTGT TTATGCACCA CTTTGCAATA AACAAGAGAT CTTCTATACA 
GCTATTGTGA ACCGCACAAT TGCAAACATG TTTGGATCCT GTGAGAAGGA AACAGTTGAG 
CTAAGTCCTA CTGGAAGACC AAAACGACGA AGTAGAAAGT CAATAAATTA CAGCGAACTA 
GATCAGTTCC CTAGTGAATT AGAAAAACTA ATAAGTCAAA TACAGCCAGA AGTAAACAGA 
GAGAGGACTG TTGTGGAAGG TAATATCCCT ATAGAATCTG AAGTTAATCT GAAGCTGCGG 
AATATAATGA TGCTACTTCG AAAGTGCTGT AATCACCCAT ATATGATTGA ATATCCTATT 
GACCCTGTCA CACAAGAATT TAAGATTGAT GAAGAGTTGG TAACAAATTC TGGGAAGTTC 
TTAATTTTGG ATCGAATGCT GCCCGAACTT AAAAAAAGAG GTCACAAGGT TCTGGTGTTT 
TCACAAATGA CAAGCATGTT GGACATCTTG ATGGATTATT GCCATCTTAG AAACTTCATC 
TTTAGCAGAC TTGATGGGTC CATGTCTTAT TCGGAAAGAG AAAAAAATAT ATACAGTTTC 
AACACGGATC CAGATGTTTT TCTTTTCTTA GTGAGTACAC GAGCTGGTGG TTTAGGCATA 
AACTTGACTG CAGCAGATAC AGTGATCATT TATGACAGTG ATTGGAATCC TCAGTCTGAT 
CTCCAGGCTC AAGATAGATG TCATAGAATT GGTCAGACAA AGCCAGTTGT TGTGTATCGT 
CTTGTAACAG CAAATACTAT TGACCAGAAA ATTGTGGAAA GAGCAGCTGC TAAAAGAAAG 
TTGGAGAAGT TGATAATCCA TAAAAATCAT TTCAAAGGTG GTCAGTCTGG GTTAAGTCAA 
TCTAAGAATT TCTTAGATGC AAAGGAATTA ATGGAGTTAT TAAAATCTAG AGATTATGAA 
AGGGAAGTAA AAGGATCCAG AGAGAAGGTC ATTAGTGACG AAGATCTAGA ATTACTGTTG 
GATCGAAGTG ATCTCATTGA TCAAATGAAG GCTTCAAGAC CAATTAAAGG GAAGACAGGG 
ATATTCAAAA TACTAGAAAA TTCTGAAGAT TCCAGTGCTG AATGTTTATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hells ( NM_008234.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032260.2 Mus musculus helicase, lymphoid specific (Hells), mRNA.

Price & Availability↑

CloneID	OMu15031
Clone ID Related Accession (Same CDS sequence)	XM_006526699.4 , NM_008234.3 , XM_006526699.3
Accession Version	NM_008234.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	lymphocyte-specific helicase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF155210.1. On Feb 16, 2006 this sequence version replaced NM_008234.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF155210.1, BC100394.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAAC AAACGGAGCC TGCGGTGATT ACTCCAGCCA TGCTGGAGGA GGAGGAACAG 
CTCGAGGCTG CGGGACTTGA GAAAGAGCGG AAGATGTTAG AAGAGGCTCA AAAGTCTTGG 
GACAGAGAGT CCACTGAAAT TCGGTACCGC AGACTTCAGC ATTTGCTTGA AAAAAGCAAT 
ATCTACTCAA AATTTTTGTT GACTAAAATG GAACAACAGC AATTGGAGGA ACAGAAGAAG 
AAAGAGAAGT TGGAGAAAAA AAAACGGTCA TTAAAACTTA CAGAGGGTAA AAGTTTAGTT 
GATGGGAATG GAGAGAAACC AGTCATGAAA AAGAAAAGAG GAAGAGAAGA TGAATCTTAT 
AATATTTCAG AGGTCATGTC AAAAGAGGAA ATTTTGTCCG TAGCTAAAAA ACATAAGGAT 
AATGAGGATG AAAGCTCTTC CACTACGAGT CTTTGTGTAG AAGATATTCA GAAAAATAAG 
GATTCAAATA GTATGATTAA AGATAGATTG TCTCAAACTG TTAGGCAGAA TTCTAAATTC 
TTTTTTGACC CAGTTCGGAA ATGTAACGGA CAGCCTGTAC CCTTTCAACA ACCAAAGCAT 
TTCACAGGAG GAGTAATGAG GTGGTACCAA GTAGAAGGCA TGGAATGGCT TAGGATGCTT 
TGGGAAAATG GAATTAATGG CATTTTAGCA GATGAAATGG GTTTGGGAAA GACTGTTCAG 
TGCATTGCTA CAATTGCATT GATGATTCAG AGGGGAGTAC CTGGACCTTT TCTTGTTTGT 
GGCCCTTTGT CTACACTTCC TAACTGGATG GCTGAATTTA AAAGATTTAC TCCAGAAATT 
CCTACTCTGC TGTATCATGG AACCCGGGAG GACCGTCGGA AATTAGTAAA GAATATCCAC 
AAAAGACAAG GGACACTGCA GATTCATCCT GTGGTGGTCA CATCATTCGA GATCGCTATG 
CGAGACCAGA ATGCTTTACA GCATTGCTAT TGGAAATACT TAATAGTAGA TGAAGGACAC 
AGGATTAAGA ATATGAAGTG CCGGCTAATC AGGGAGTTAA AACGGTTCAA TGCTGATAAC 
AAACTTCTTT TGACTGGTAC TCCCTTGCAA AACAATTTAT CAGAACTTTG GTCATTGCTA 
AACTTTTTGT TGCCAGATGT ATTTGATGAC TTGAAAAGCT TTGAGTCTTG GTTTGATATC 
ACTAGTCTTT CTGAAACTGC TGAAGATATT ATAGCTAAAG AGAGAGAACA GAATGTTTTA 
CATATGCTGC ACCAGATTTT AACGCCTTTC CTACTGAGAA GACTGAAGTC TGATGTTGCT 
CTTGAAGTCC CCCCTAAGCG AGAAGTAGTT GTTTATGCAC CACTTTGCAA TAAACAAGAG 
ATCTTCTATA CAGCTATTGT GAACCGCACA ATTGCAAACA TGTTTGGATC CTGTGAGAAG 
GAAACAGTTG AGCTAAGTCC TACTGGAAGA CCAAAACGAC GAAGTAGAAA GTCAATAAAT 
TACAGCGAAC TAGATCAGTT CCCTAGTGAA TTAGAAAAAC TAATAAGTCA AATACAGCCA 
GAAGTAAACA GAGAGAGGAC TGTTGTGGAA GGTAATATCC CTATAGAATC TGAAGTTAAT 
CTGAAGCTGC GGAATATAAT GATGCTACTT CGAAAGTGCT GTAATCACCC ATATATGATT 
GAATATCCTA TTGACCCTGT CACACAAGAA TTTAAGATTG ATGAAGAGTT GGTAACAAAT 
TCTGGGAAGT TCTTAATTTT GGATCGAATG CTGCCCGAAC TTAAAAAAAG AGGTCACAAG 
GTTCTGGTGT TTTCACAAAT GACAAGCATG TTGGACATCT TGATGGATTA TTGCCATCTT 
AGAAACTTCA TCTTTAGCAG ACTTGATGGG TCCATGTCTT ATTCGGAAAG AGAAAAAAAT 
ATATACAGTT TCAACACGGA TCCAGATGTT TTTCTTTTCT TAGTGAGTAC ACGAGCTGGT 
GGTTTAGGCA TAAACTTGAC TGCAGCAGAT ACAGTGATCA TTTATGACAG TGATTGGAAT 
CCTCAGTCTG ATCTCCAGGC TCAAGATAGA TGTCATAGAA TTGGTCAGAC AAAGCCAGTT 
GTTGTGTATC GTCTTGTAAC AGCAAATACT ATTGACCAGA AAATTGTGGA AAGAGCAGCT 
GCTAAAAGAA AGTTGGAGAA GTTGATAATC CATAAAAATC ATTTCAAAGG TGGTCAGTCT 
GGGTTAAGTC AATCTAAGAA TTTCTTAGAT GCAAAGGAAT TAATGGAGTT ATTAAAATCT 
AGAGATTATG AAAGGGAAGT AAAAGGATCC AGAGAGAAGG TCATTAGTGA CGAAGATCTA 
GAATTACTGT TGGATCGAAG TGATCTCATT GATCAAATGA AGGCTTCAAG ACCAATTAAA 
GGGAAGACAG GGATATTCAA AATACTAGAA AATTCTGAAG ATTCCAGTGC TGAATGTTTA 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032260.2
CDS	104..2569
Translation	MAEQTEPAVITPAMLEEEEQLEAAGLEKERKMLEEAQKSWDRESTEIRYRRLQHLLEKSNIYSKFLLTKMEQQQLEEQKKKEKLEKKKRSLKLTEGKSLVDGNGEKPVMKKKRGREDESYNISEVMSKEEILSVAKKHKDNEDESSSTTSLCVEDIQKNKDSNSMIKDRLSQTVRQNSKFFFDPVRKCNGQPVPFQQPKHFTGGVMRWYQVEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLVCGPLSTLPNWMAEFKRFTPEIPTLLYHGTREDRRKLVKNIHKRQGTLQIHPVVVTSFEIAMRDQNALQHCYWKYLIVDEGHRIKNMKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSLSETAEDIIAKEREQNVLHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLCNKQEIFYTAIVNRTIANMFGSCEKETVELSPTGRPKRRSRKSINYSELDQFPSELEKLISQIQPEVNRERTVVEGNIPIESEVNLKLRNIMMLLRKCCNHPYMIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLVFSQMTSMLDILMDYCHLRNFIFSRLDGSMSYSEREKNIYSFNTDPDVFLFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLSQSKNFLDAKELMELLKSRDYEREVKGSREKVISDEDLELLLDRSDLIDQMKASRPIKGKTGIFKILENSEDSSAECLF

Target ORF information:

RefSeq Version	NM_008234.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus helicase, lymphoid specific (Hells), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008234.3

ORF Insert Sequence:

ATGGCCGAAC AAACGGAGCC TGCGGTGATT ACTCCAGCCA TGCTGGAGGA GGAGGAACAG 
CTCGAGGCTG CGGGACTTGA GAAAGAGCGG AAGATGTTAG AAGAGGCTCA AAAGTCTTGG 
GACAGAGAGT CCACTGAAAT TCGGTACCGC AGACTTCAGC ATTTGCTTGA AAAAAGCAAT 
ATCTACTCAA AATTTTTGTT GACTAAAATG GAACAACAGC AATTGGAGGA ACAGAAGAAG 
AAAGAGAAGT TGGAGAAAAA AAAACGGTCA TTAAAACTTA CAGAGGGTAA AAGTTTAGTT 
GATGGGAATG GAGAGAAACC AGTCATGAAA AAGAAAAGAG GAAGAGAAGA TGAATCTTAT 
AATATTTCAG AGGTCATGTC AAAAGAGGAA ATTTTGTCCG TAGCTAAAAA ACATAAGGAT 
AATGAGGATG AAAGCTCTTC CACTACGAGT CTTTGTGTAG AAGATATTCA GAAAAATAAG 
GATTCAAATA GTATGATTAA AGATAGATTG TCTCAAACTG TTAGGCAGAA TTCTAAATTC 
TTTTTTGACC CAGTTCGGAA ATGTAACGGA CAGCCTGTAC CCTTTCAACA ACCAAAGCAT 
TTCACAGGAG GAGTAATGAG GTGGTACCAA GTAGAAGGCA TGGAATGGCT TAGGATGCTT 
TGGGAAAATG GAATTAATGG CATTTTAGCA GATGAAATGG GTTTGGGAAA GACTGTTCAG 
TGCATTGCTA CAATTGCATT GATGATTCAG AGGGGAGTAC CTGGACCTTT TCTTGTTTGT 
GGCCCTTTGT CTACACTTCC TAACTGGATG GCTGAATTTA AAAGATTTAC TCCAGAAATT 
CCTACTCTGC TGTATCATGG AACCCGGGAG GACCGTCGGA AATTAGTAAA GAATATCCAC 
AAAAGACAAG GGACACTGCA GATTCATCCT GTGGTGGTCA CATCATTCGA GATCGCTATG 
CGAGACCAGA ATGCTTTACA GCATTGCTAT TGGAAATACT TAATAGTAGA TGAAGGACAC 
AGGATTAAGA ATATGAAGTG CCGGCTAATC AGGGAGTTAA AACGGTTCAA TGCTGATAAC 
AAACTTCTTT TGACTGGTAC TCCCTTGCAA AACAATTTAT CAGAACTTTG GTCATTGCTA 
AACTTTTTGT TGCCAGATGT ATTTGATGAC TTGAAAAGCT TTGAGTCTTG GTTTGATATC 
ACTAGTCTTT CTGAAACTGC TGAAGATATT ATAGCTAAAG AGAGAGAACA GAATGTTTTA 
CATATGCTGC ACCAGATTTT AACGCCTTTC CTACTGAGAA GACTGAAGTC TGATGTTGCT 
CTTGAAGTCC CCCCTAAGCG AGAAGTAGTT GTTTATGCAC CACTTTGCAA TAAACAAGAG 
ATCTTCTATA CAGCTATTGT GAACCGCACA ATTGCAAACA TGTTTGGATC CTGTGAGAAG 
GAAACAGTTG AGCTAAGTCC TACTGGAAGA CCAAAACGAC GAAGTAGAAA GTCAATAAAT 
TACAGCGAAC TAGATCAGTT CCCTAGTGAA TTAGAAAAAC TAATAAGTCA AATACAGCCA 
GAAGTAAACA GAGAGAGGAC TGTTGTGGAA GGTAATATCC CTATAGAATC TGAAGTTAAT 
CTGAAGCTGC GGAATATAAT GATGCTACTT CGAAAGTGCT GTAATCACCC ATATATGATT 
GAATATCCTA TTGACCCTGT CACACAAGAA TTTAAGATTG ATGAAGAGTT GGTAACAAAT 
TCTGGGAAGT TCTTAATTTT GGATCGAATG CTGCCCGAAC TTAAAAAAAG AGGTCACAAG 
GTTCTGGTGT TTTCACAAAT GACAAGCATG TTGGACATCT TGATGGATTA TTGCCATCTT 
AGAAACTTCA TCTTTAGCAG ACTTGATGGG TCCATGTCTT ATTCGGAAAG AGAAAAAAAT 
ATATACAGTT TCAACACGGA TCCAGATGTT TTTCTTTTCT TAGTGAGTAC ACGAGCTGGT 
GGTTTAGGCA TAAACTTGAC TGCAGCAGAT ACAGTGATCA TTTATGACAG TGATTGGAAT 
CCTCAGTCTG ATCTCCAGGC TCAAGATAGA TGTCATAGAA TTGGTCAGAC AAAGCCAGTT 
GTTGTGTATC GTCTTGTAAC AGCAAATACT ATTGACCAGA AAATTGTGGA AAGAGCAGCT 
GCTAAAAGAA AGTTGGAGAA GTTGATAATC CATAAAAATC ATTTCAAAGG TGGTCAGTCT 
GGGTTAAGTC AATCTAAGAA TTTCTTAGAT GCAAAGGAAT TAATGGAGTT ATTAAAATCT 
AGAGATTATG AAAGGGAAGT AAAAGGATCC AGAGAGAAGG TCATTAGTGA CGAAGATCTA 
GAATTACTGT TGGATCGAAG TGATCTCATT GATCAAATGA AGGCTTCAAG ACCAATTAAA 
GGGAAGACAG GGATATTCAA AATACTAGAA AATTCTGAAG ATTCCAGTGC TGAATGTTTA 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hells ( XM_006526699.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526762.1 Mus musculus helicase, lymphoid specific (Hells), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15031
Clone ID Related Accession (Same CDS sequence)	XM_006526699.4 , NM_008234.3 , XM_006526699.3
Accession Version	XM_006526699.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lymphocyte-specific helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAAC AAACGGAGCC TGCGGTGATT ACTCCAGCCA TGCTGGAGGA GGAGGAACAG 
CTCGAGGCTG CGGGACTTGA GAAAGAGCGG AAGATGTTAG AAGAGGCTCA AAAGTCTTGG 
GACAGAGAGT CCACTGAAAT TCGGTACCGC AGACTTCAGC ATTTGCTTGA AAAAAGCAAT 
ATCTACTCAA AATTTTTGTT GACTAAAATG GAACAACAGC AATTGGAGGA ACAGAAGAAG 
AAAGAGAAGT TGGAGAAAAA AAAACGGTCA TTAAAACTTA CAGAGGGTAA AAGTTTAGTT 
GATGGGAATG GAGAGAAACC AGTCATGAAA AAGAAAAGAG GAAGAGAAGA TGAATCTTAT 
AATATTTCAG AGGTCATGTC AAAAGAGGAA ATTTTGTCCG TAGCTAAAAA ACATAAGGAT 
AATGAGGATG AAAGCTCTTC CACTACGAGT CTTTGTGTAG AAGATATTCA GAAAAATAAG 
GATTCAAATA GTATGATTAA AGATAGATTG TCTCAAACTG TTAGGCAGAA TTCTAAATTC 
TTTTTTGACC CAGTTCGGAA ATGTAACGGA CAGCCTGTAC CCTTTCAACA ACCAAAGCAT 
TTCACAGGAG GAGTAATGAG GTGGTACCAA GTAGAAGGCA TGGAATGGCT TAGGATGCTT 
TGGGAAAATG GAATTAATGG CATTTTAGCA GATGAAATGG GTTTGGGAAA GACTGTTCAG 
TGCATTGCTA CAATTGCATT GATGATTCAG AGGGGAGTAC CTGGACCTTT TCTTGTTTGT 
GGCCCTTTGT CTACACTTCC TAACTGGATG GCTGAATTTA AAAGATTTAC TCCAGAAATT 
CCTACTCTGC TGTATCATGG AACCCGGGAG GACCGTCGGA AATTAGTAAA GAATATCCAC 
AAAAGACAAG GGACACTGCA GATTCATCCT GTGGTGGTCA CATCATTCGA GATCGCTATG 
CGAGACCAGA ATGCTTTACA GCATTGCTAT TGGAAATACT TAATAGTAGA TGAAGGACAC 
AGGATTAAGA ATATGAAGTG CCGGCTAATC AGGGAGTTAA AACGGTTCAA TGCTGATAAC 
AAACTTCTTT TGACTGGTAC TCCCTTGCAA AACAATTTAT CAGAACTTTG GTCATTGCTA 
AACTTTTTGT TGCCAGATGT ATTTGATGAC TTGAAAAGCT TTGAGTCTTG GTTTGATATC 
ACTAGTCTTT CTGAAACTGC TGAAGATATT ATAGCTAAAG AGAGAGAACA GAATGTTTTA 
CATATGCTGC ACCAGATTTT AACGCCTTTC CTACTGAGAA GACTGAAGTC TGATGTTGCT 
CTTGAAGTCC CCCCTAAGCG AGAAGTAGTT GTTTATGCAC CACTTTGCAA TAAACAAGAG 
ATCTTCTATA CAGCTATTGT GAACCGCACA ATTGCAAACA TGTTTGGATC CTGTGAGAAG 
GAAACAGTTG AGCTAAGTCC TACTGGAAGA CCAAAACGAC GAAGTAGAAA GTCAATAAAT 
TACAGCGAAC TAGATCAGTT CCCTAGTGAA TTAGAAAAAC TAATAAGTCA AATACAGCCA 
GAAGTAAACA GAGAGAGGAC TGTTGTGGAA GGTAATATCC CTATAGAATC TGAAGTTAAT 
CTGAAGCTGC GGAATATAAT GATGCTACTT CGAAAGTGCT GTAATCACCC ATATATGATT 
GAATATCCTA TTGACCCTGT CACACAAGAA TTTAAGATTG ATGAAGAGTT GGTAACAAAT 
TCTGGGAAGT TCTTAATTTT GGATCGAATG CTGCCCGAAC TTAAAAAAAG AGGTCACAAG 
GTTCTGGTGT TTTCACAAAT GACAAGCATG TTGGACATCT TGATGGATTA TTGCCATCTT 
AGAAACTTCA TCTTTAGCAG ACTTGATGGG TCCATGTCTT ATTCGGAAAG AGAAAAAAAT 
ATATACAGTT TCAACACGGA TCCAGATGTT TTTCTTTTCT TAGTGAGTAC ACGAGCTGGT 
GGTTTAGGCA TAAACTTGAC TGCAGCAGAT ACAGTGATCA TTTATGACAG TGATTGGAAT 
CCTCAGTCTG ATCTCCAGGC TCAAGATAGA TGTCATAGAA TTGGTCAGAC AAAGCCAGTT 
GTTGTGTATC GTCTTGTAAC AGCAAATACT ATTGACCAGA AAATTGTGGA AAGAGCAGCT 
GCTAAAAGAA AGTTGGAGAA GTTGATAATC CATAAAAATC ATTTCAAAGG TGGTCAGTCT 
GGGTTAAGTC AATCTAAGAA TTTCTTAGAT GCAAAGGAAT TAATGGAGTT ATTAAAATCT 
AGAGATTATG AAAGGGAAGT AAAAGGATCC AGAGAGAAGG TCATTAGTGA CGAAGATCTA 
GAATTACTGT TGGATCGAAG TGATCTCATT GATCAAATGA AGGCTTCAAG ACCAATTAAA 
GGGAAGACAG GGATATTCAA AATACTAGAA AATTCTGAAG ATTCCAGTGC TGAATGTTTA 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526762.1
CDS	147..2612
Translation	MAEQTEPAVITPAMLEEEEQLEAAGLEKERKMLEEAQKSWDRESTEIRYRRLQHLLEKSNIYSKFLLTKMEQQQLEEQKKKEKLEKKKRSLKLTEGKSLVDGNGEKPVMKKKRGREDESYNISEVMSKEEILSVAKKHKDNEDESSSTTSLCVEDIQKNKDSNSMIKDRLSQTVRQNSKFFFDPVRKCNGQPVPFQQPKHFTGGVMRWYQVEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLVCGPLSTLPNWMAEFKRFTPEIPTLLYHGTREDRRKLVKNIHKRQGTLQIHPVVVTSFEIAMRDQNALQHCYWKYLIVDEGHRIKNMKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSLSETAEDIIAKEREQNVLHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLCNKQEIFYTAIVNRTIANMFGSCEKETVELSPTGRPKRRSRKSINYSELDQFPSELEKLISQIQPEVNRERTVVEGNIPIESEVNLKLRNIMMLLRKCCNHPYMIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLVFSQMTSMLDILMDYCHLRNFIFSRLDGSMSYSEREKNIYSFNTDPDVFLFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLSQSKNFLDAKELMELLKSRDYEREVKGSREKVISDEDLELLLDRSDLIDQMKASRPIKGKTGIFKILENSEDSSAECLF

Target ORF information:

RefSeq Version	XM_006526699.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus helicase, lymphoid specific (Hells), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526699.3

ORF Insert Sequence:

ATGGCCGAAC AAACGGAGCC TGCGGTGATT ACTCCAGCCA TGCTGGAGGA GGAGGAACAG 
CTCGAGGCTG CGGGACTTGA GAAAGAGCGG AAGATGTTAG AAGAGGCTCA AAAGTCTTGG 
GACAGAGAGT CCACTGAAAT TCGGTACCGC AGACTTCAGC ATTTGCTTGA AAAAAGCAAT 
ATCTACTCAA AATTTTTGTT GACTAAAATG GAACAACAGC AATTGGAGGA ACAGAAGAAG 
AAAGAGAAGT TGGAGAAAAA AAAACGGTCA TTAAAACTTA CAGAGGGTAA AAGTTTAGTT 
GATGGGAATG GAGAGAAACC AGTCATGAAA AAGAAAAGAG GAAGAGAAGA TGAATCTTAT 
AATATTTCAG AGGTCATGTC AAAAGAGGAA ATTTTGTCCG TAGCTAAAAA ACATAAGGAT 
AATGAGGATG AAAGCTCTTC CACTACGAGT CTTTGTGTAG AAGATATTCA GAAAAATAAG 
GATTCAAATA GTATGATTAA AGATAGATTG TCTCAAACTG TTAGGCAGAA TTCTAAATTC 
TTTTTTGACC CAGTTCGGAA ATGTAACGGA CAGCCTGTAC CCTTTCAACA ACCAAAGCAT 
TTCACAGGAG GAGTAATGAG GTGGTACCAA GTAGAAGGCA TGGAATGGCT TAGGATGCTT 
TGGGAAAATG GAATTAATGG CATTTTAGCA GATGAAATGG GTTTGGGAAA GACTGTTCAG 
TGCATTGCTA CAATTGCATT GATGATTCAG AGGGGAGTAC CTGGACCTTT TCTTGTTTGT 
GGCCCTTTGT CTACACTTCC TAACTGGATG GCTGAATTTA AAAGATTTAC TCCAGAAATT 
CCTACTCTGC TGTATCATGG AACCCGGGAG GACCGTCGGA AATTAGTAAA GAATATCCAC 
AAAAGACAAG GGACACTGCA GATTCATCCT GTGGTGGTCA CATCATTCGA GATCGCTATG 
CGAGACCAGA ATGCTTTACA GCATTGCTAT TGGAAATACT TAATAGTAGA TGAAGGACAC 
AGGATTAAGA ATATGAAGTG CCGGCTAATC AGGGAGTTAA AACGGTTCAA TGCTGATAAC 
AAACTTCTTT TGACTGGTAC TCCCTTGCAA AACAATTTAT CAGAACTTTG GTCATTGCTA 
AACTTTTTGT TGCCAGATGT ATTTGATGAC TTGAAAAGCT TTGAGTCTTG GTTTGATATC 
ACTAGTCTTT CTGAAACTGC TGAAGATATT ATAGCTAAAG AGAGAGAACA GAATGTTTTA 
CATATGCTGC ACCAGATTTT AACGCCTTTC CTACTGAGAA GACTGAAGTC TGATGTTGCT 
CTTGAAGTCC CCCCTAAGCG AGAAGTAGTT GTTTATGCAC CACTTTGCAA TAAACAAGAG 
ATCTTCTATA CAGCTATTGT GAACCGCACA ATTGCAAACA TGTTTGGATC CTGTGAGAAG 
GAAACAGTTG AGCTAAGTCC TACTGGAAGA CCAAAACGAC GAAGTAGAAA GTCAATAAAT 
TACAGCGAAC TAGATCAGTT CCCTAGTGAA TTAGAAAAAC TAATAAGTCA AATACAGCCA 
GAAGTAAACA GAGAGAGGAC TGTTGTGGAA GGTAATATCC CTATAGAATC TGAAGTTAAT 
CTGAAGCTGC GGAATATAAT GATGCTACTT CGAAAGTGCT GTAATCACCC ATATATGATT 
GAATATCCTA TTGACCCTGT CACACAAGAA TTTAAGATTG ATGAAGAGTT GGTAACAAAT 
TCTGGGAAGT TCTTAATTTT GGATCGAATG CTGCCCGAAC TTAAAAAAAG AGGTCACAAG 
GTTCTGGTGT TTTCACAAAT GACAAGCATG TTGGACATCT TGATGGATTA TTGCCATCTT 
AGAAACTTCA TCTTTAGCAG ACTTGATGGG TCCATGTCTT ATTCGGAAAG AGAAAAAAAT 
ATATACAGTT TCAACACGGA TCCAGATGTT TTTCTTTTCT TAGTGAGTAC ACGAGCTGGT 
GGTTTAGGCA TAAACTTGAC TGCAGCAGAT ACAGTGATCA TTTATGACAG TGATTGGAAT 
CCTCAGTCTG ATCTCCAGGC TCAAGATAGA TGTCATAGAA TTGGTCAGAC AAAGCCAGTT 
GTTGTGTATC GTCTTGTAAC AGCAAATACT ATTGACCAGA AAATTGTGGA AAGAGCAGCT 
GCTAAAAGAA AGTTGGAGAA GTTGATAATC CATAAAAATC ATTTCAAAGG TGGTCAGTCT 
GGGTTAAGTC AATCTAAGAA TTTCTTAGAT GCAAAGGAAT TAATGGAGTT ATTAAAATCT 
AGAGATTATG AAAGGGAAGT AAAAGGATCC AGAGAGAAGG TCATTAGTGA CGAAGATCTA 
GAATTACTGT TGGATCGAAG TGATCTCATT GATCAAATGA AGGCTTCAAG ACCAATTAAA 
GGGAAGACAG GGATATTCAA AATACTAGAA AATTCTGAAG ATTCCAGTGC TGAATGTTTA 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hells ( XM_006526699.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526762.1 Mus musculus helicase, lymphoid specific (Hells), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15031
Clone ID Related Accession (Same CDS sequence)	XM_006526699.4 , NM_008234.3 , XM_006526699.3
Accession Version	XM_006526699.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lymphocyte-specific helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526699.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAAC AAACGGAGCC TGCGGTGATT ACTCCAGCCA TGCTGGAGGA GGAGGAACAG 
CTCGAGGCTG CGGGACTTGA GAAAGAGCGG AAGATGTTAG AAGAGGCTCA AAAGTCTTGG 
GACAGAGAGT CCACTGAAAT TCGGTACCGC AGACTTCAGC ATTTGCTTGA AAAAAGCAAT 
ATCTACTCAA AATTTTTGTT GACTAAAATG GAACAACAGC AATTGGAGGA ACAGAAGAAG 
AAAGAGAAGT TGGAGAAAAA AAAACGGTCA TTAAAACTTA CAGAGGGTAA AAGTTTAGTT 
GATGGGAATG GAGAGAAACC AGTCATGAAA AAGAAAAGAG GAAGAGAAGA TGAATCTTAT 
AATATTTCAG AGGTCATGTC AAAAGAGGAA ATTTTGTCCG TAGCTAAAAA ACATAAGGAT 
AATGAGGATG AAAGCTCTTC CACTACGAGT CTTTGTGTAG AAGATATTCA GAAAAATAAG 
GATTCAAATA GTATGATTAA AGATAGATTG TCTCAAACTG TTAGGCAGAA TTCTAAATTC 
TTTTTTGACC CAGTTCGGAA ATGTAACGGA CAGCCTGTAC CCTTTCAACA ACCAAAGCAT 
TTCACAGGAG GAGTAATGAG GTGGTACCAA GTAGAAGGCA TGGAATGGCT TAGGATGCTT 
TGGGAAAATG GAATTAATGG CATTTTAGCA GATGAAATGG GTTTGGGAAA GACTGTTCAG 
TGCATTGCTA CAATTGCATT GATGATTCAG AGGGGAGTAC CTGGACCTTT TCTTGTTTGT 
GGCCCTTTGT CTACACTTCC TAACTGGATG GCTGAATTTA AAAGATTTAC TCCAGAAATT 
CCTACTCTGC TGTATCATGG AACCCGGGAG GACCGTCGGA AATTAGTAAA GAATATCCAC 
AAAAGACAAG GGACACTGCA GATTCATCCT GTGGTGGTCA CATCATTCGA GATCGCTATG 
CGAGACCAGA ATGCTTTACA GCATTGCTAT TGGAAATACT TAATAGTAGA TGAAGGACAC 
AGGATTAAGA ATATGAAGTG CCGGCTAATC AGGGAGTTAA AACGGTTCAA TGCTGATAAC 
AAACTTCTTT TGACTGGTAC TCCCTTGCAA AACAATTTAT CAGAACTTTG GTCATTGCTA 
AACTTTTTGT TGCCAGATGT ATTTGATGAC TTGAAAAGCT TTGAGTCTTG GTTTGATATC 
ACTAGTCTTT CTGAAACTGC TGAAGATATT ATAGCTAAAG AGAGAGAACA GAATGTTTTA 
CATATGCTGC ACCAGATTTT AACGCCTTTC CTACTGAGAA GACTGAAGTC TGATGTTGCT 
CTTGAAGTCC CCCCTAAGCG AGAAGTAGTT GTTTATGCAC CACTTTGCAA TAAACAAGAG 
ATCTTCTATA CAGCTATTGT GAACCGCACA ATTGCAAACA TGTTTGGATC CTGTGAGAAG 
GAAACAGTTG AGCTAAGTCC TACTGGAAGA CCAAAACGAC GAAGTAGAAA GTCAATAAAT 
TACAGCGAAC TAGATCAGTT CCCTAGTGAA TTAGAAAAAC TAATAAGTCA AATACAGCCA 
GAAGTAAACA GAGAGAGGAC TGTTGTGGAA GGTAATATCC CTATAGAATC TGAAGTTAAT 
CTGAAGCTGC GGAATATAAT GATGCTACTT CGAAAGTGCT GTAATCACCC ATATATGATT 
GAATATCCTA TTGACCCTGT CACACAAGAA TTTAAGATTG ATGAAGAGTT GGTAACAAAT 
TCTGGGAAGT TCTTAATTTT GGATCGAATG CTGCCCGAAC TTAAAAAAAG AGGTCACAAG 
GTTCTGGTGT TTTCACAAAT GACAAGCATG TTGGACATCT TGATGGATTA TTGCCATCTT 
AGAAACTTCA TCTTTAGCAG ACTTGATGGG TCCATGTCTT ATTCGGAAAG AGAAAAAAAT 
ATATACAGTT TCAACACGGA TCCAGATGTT TTTCTTTTCT TAGTGAGTAC ACGAGCTGGT 
GGTTTAGGCA TAAACTTGAC TGCAGCAGAT ACAGTGATCA TTTATGACAG TGATTGGAAT 
CCTCAGTCTG ATCTCCAGGC TCAAGATAGA TGTCATAGAA TTGGTCAGAC AAAGCCAGTT 
GTTGTGTATC GTCTTGTAAC AGCAAATACT ATTGACCAGA AAATTGTGGA AAGAGCAGCT 
GCTAAAAGAA AGTTGGAGAA GTTGATAATC CATAAAAATC ATTTCAAAGG TGGTCAGTCT 
GGGTTAAGTC AATCTAAGAA TTTCTTAGAT GCAAAGGAAT TAATGGAGTT ATTAAAATCT 
AGAGATTATG AAAGGGAAGT AAAAGGATCC AGAGAGAAGG TCATTAGTGA CGAAGATCTA 
GAATTACTGT TGGATCGAAG TGATCTCATT GATCAAATGA AGGCTTCAAG ACCAATTAAA 
GGGAAGACAG GGATATTCAA AATACTAGAA AATTCTGAAG ATTCCAGTGC TGAATGTTTA 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526762.1
CDS	975..3440
Translation	MAEQTEPAVITPAMLEEEEQLEAAGLEKERKMLEEAQKSWDRESTEIRYRRLQHLLEKSNIYSKFLLTKMEQQQLEEQKKKEKLEKKKRSLKLTEGKSLVDGNGEKPVMKKKRGREDESYNISEVMSKEEILSVAKKHKDNEDESSSTTSLCVEDIQKNKDSNSMIKDRLSQTVRQNSKFFFDPVRKCNGQPVPFQQPKHFTGGVMRWYQVEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLVCGPLSTLPNWMAEFKRFTPEIPTLLYHGTREDRRKLVKNIHKRQGTLQIHPVVVTSFEIAMRDQNALQHCYWKYLIVDEGHRIKNMKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSLSETAEDIIAKEREQNVLHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLCNKQEIFYTAIVNRTIANMFGSCEKETVELSPTGRPKRRSRKSINYSELDQFPSELEKLISQIQPEVNRERTVVEGNIPIESEVNLKLRNIMMLLRKCCNHPYMIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLVFSQMTSMLDILMDYCHLRNFIFSRLDGSMSYSEREKNIYSFNTDPDVFLFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLSQSKNFLDAKELMELLKSRDYEREVKGSREKVISDEDLELLLDRSDLIDQMKASRPIKGKTGIFKILENSEDSSAECLF

Target ORF information:

RefSeq Version	XM_006526699.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus helicase, lymphoid specific (Hells), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526699.4

ORF Insert Sequence:

ATGGCCGAAC AAACGGAGCC TGCGGTGATT ACTCCAGCCA TGCTGGAGGA GGAGGAACAG 
CTCGAGGCTG CGGGACTTGA GAAAGAGCGG AAGATGTTAG AAGAGGCTCA AAAGTCTTGG 
GACAGAGAGT CCACTGAAAT TCGGTACCGC AGACTTCAGC ATTTGCTTGA AAAAAGCAAT 
ATCTACTCAA AATTTTTGTT GACTAAAATG GAACAACAGC AATTGGAGGA ACAGAAGAAG 
AAAGAGAAGT TGGAGAAAAA AAAACGGTCA TTAAAACTTA CAGAGGGTAA AAGTTTAGTT 
GATGGGAATG GAGAGAAACC AGTCATGAAA AAGAAAAGAG GAAGAGAAGA TGAATCTTAT 
AATATTTCAG AGGTCATGTC AAAAGAGGAA ATTTTGTCCG TAGCTAAAAA ACATAAGGAT 
AATGAGGATG AAAGCTCTTC CACTACGAGT CTTTGTGTAG AAGATATTCA GAAAAATAAG 
GATTCAAATA GTATGATTAA AGATAGATTG TCTCAAACTG TTAGGCAGAA TTCTAAATTC 
TTTTTTGACC CAGTTCGGAA ATGTAACGGA CAGCCTGTAC CCTTTCAACA ACCAAAGCAT 
TTCACAGGAG GAGTAATGAG GTGGTACCAA GTAGAAGGCA TGGAATGGCT TAGGATGCTT 
TGGGAAAATG GAATTAATGG CATTTTAGCA GATGAAATGG GTTTGGGAAA GACTGTTCAG 
TGCATTGCTA CAATTGCATT GATGATTCAG AGGGGAGTAC CTGGACCTTT TCTTGTTTGT 
GGCCCTTTGT CTACACTTCC TAACTGGATG GCTGAATTTA AAAGATTTAC TCCAGAAATT 
CCTACTCTGC TGTATCATGG AACCCGGGAG GACCGTCGGA AATTAGTAAA GAATATCCAC 
AAAAGACAAG GGACACTGCA GATTCATCCT GTGGTGGTCA CATCATTCGA GATCGCTATG 
CGAGACCAGA ATGCTTTACA GCATTGCTAT TGGAAATACT TAATAGTAGA TGAAGGACAC 
AGGATTAAGA ATATGAAGTG CCGGCTAATC AGGGAGTTAA AACGGTTCAA TGCTGATAAC 
AAACTTCTTT TGACTGGTAC TCCCTTGCAA AACAATTTAT CAGAACTTTG GTCATTGCTA 
AACTTTTTGT TGCCAGATGT ATTTGATGAC TTGAAAAGCT TTGAGTCTTG GTTTGATATC 
ACTAGTCTTT CTGAAACTGC TGAAGATATT ATAGCTAAAG AGAGAGAACA GAATGTTTTA 
CATATGCTGC ACCAGATTTT AACGCCTTTC CTACTGAGAA GACTGAAGTC TGATGTTGCT 
CTTGAAGTCC CCCCTAAGCG AGAAGTAGTT GTTTATGCAC CACTTTGCAA TAAACAAGAG 
ATCTTCTATA CAGCTATTGT GAACCGCACA ATTGCAAACA TGTTTGGATC CTGTGAGAAG 
GAAACAGTTG AGCTAAGTCC TACTGGAAGA CCAAAACGAC GAAGTAGAAA GTCAATAAAT 
TACAGCGAAC TAGATCAGTT CCCTAGTGAA TTAGAAAAAC TAATAAGTCA AATACAGCCA 
GAAGTAAACA GAGAGAGGAC TGTTGTGGAA GGTAATATCC CTATAGAATC TGAAGTTAAT 
CTGAAGCTGC GGAATATAAT GATGCTACTT CGAAAGTGCT GTAATCACCC ATATATGATT 
GAATATCCTA TTGACCCTGT CACACAAGAA TTTAAGATTG ATGAAGAGTT GGTAACAAAT 
TCTGGGAAGT TCTTAATTTT GGATCGAATG CTGCCCGAAC TTAAAAAAAG AGGTCACAAG 
GTTCTGGTGT TTTCACAAAT GACAAGCATG TTGGACATCT TGATGGATTA TTGCCATCTT 
AGAAACTTCA TCTTTAGCAG ACTTGATGGG TCCATGTCTT ATTCGGAAAG AGAAAAAAAT 
ATATACAGTT TCAACACGGA TCCAGATGTT TTTCTTTTCT TAGTGAGTAC ACGAGCTGGT 
GGTTTAGGCA TAAACTTGAC TGCAGCAGAT ACAGTGATCA TTTATGACAG TGATTGGAAT 
CCTCAGTCTG ATCTCCAGGC TCAAGATAGA TGTCATAGAA TTGGTCAGAC AAAGCCAGTT 
GTTGTGTATC GTCTTGTAAC AGCAAATACT ATTGACCAGA AAATTGTGGA AAGAGCAGCT 
GCTAAAAGAA AGTTGGAGAA GTTGATAATC CATAAAAATC ATTTCAAAGG TGGTCAGTCT 
GGGTTAAGTC AATCTAAGAA TTTCTTAGAT GCAAAGGAAT TAATGGAGTT ATTAAAATCT 
AGAGATTATG AAAGGGAAGT AAAAGGATCC AGAGAGAAGG TCATTAGTGA CGAAGATCTA 
GAATTACTGT TGGATCGAAG TGATCTCATT GATCAAATGA AGGCTTCAAG ACCAATTAAA 
GGGAAGACAG GGATATTCAA AATACTAGAA AATTCTGAAG ATTCCAGTGC TGAATGTTTA 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tethering of Lsh at the Oct4 locus promotes gene repression associated with epigenetic changes.
Epigenetics0(2017 Jun)
Ren J,Hathaway NA,Crabtree GR,Muegge K

Lsh/HELLS regulates self-renewal/proliferation of neural stem/progenitor cells.
Scientific reports7(1)1136(2017 Apr)
Han Y,Ren J,Lee E,Xu X,Yu W,Muegge K

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The SNF2 family ATPase LSH promotes cell-autonomous de novo DNA methylation in somatic cells.
Nucleic acids research44(16)7592-604(2016 Sep)
Termanis A,Torrea N,Culley J,Kerr A,Ramsahoye B,Stancheva I

Genome-wide DNA methylation patterns in LSH mutant reveals de-repression of repeat elements and redundant epigenetic silencing pathways.
Genome research24(10)1613-23(2014 Oct)
Yu W,McIntosh C,Lister R,Zhu I,Han Y,Ren J,Landsman D,Lee E,Briones V,Terashima M,Leighty R,Ecker JR,Muegge K

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Hells cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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