Hbb-b2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hbb-b2
Entrez Gene ID	15130
Full Name	hemoglobin, beta adult minor chain
Synonyms	AI036344,Hbb2,Hbbt2,beta2
General protein information	Preferred Names hemoglobin, beta adult minor chain Names hemoglobin subunit beta-2 beta min beta minor globin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 E3\|7 54.85 cM
Summary	This gene encodes a beta polypeptide chain found in adult hemoglobin, which consists of a tetramer of two alpha chains and two beta chains, and which functions in the transport of oxygen to various peripheral tissues. This gene is one of a cluster of beta-hemoglobin genes that are distally regulated by a locus control region, and which are organized along the chromosome in the order of their developmental expression. In mouse, two major strain-specific haplotypes of the beta-globin gene cluster are found - a 'single' haplotype found in C57BL/-type strains, which includes two highly similar adult beta-globin genes, beta s and beta t, and a 'diffuse' haplotype found in strains such as BALB/c and 129Sv, which includes two somewhat diverse adult beta-globin genes, beta-major and beta-minor. This gene represents the beta-minor adult gene found in the 'diffuse' haplotype. Primary chromosome 7 of the mouse reference genome assembly, which is derived from C57BL/6 strain mice, represents the 'single' haplotype, while the 'diffuse' haplotype is represented in the reference genome collection by the BALB/c strain alternate contig, NT_095534.1. [provided by RefSeq, May 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_016956.3	NP_058652.1	hemoglobin subunit beta-2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An altered REDOX environment, assisted by over-expression of fetal hemoglobins, protects from inflammatory colitis and reduces inflammatory cytokine expression.
Gorczynski RM, Alexander C, Brandenburg K, Chen Z, Heini A, Neumann D, Mach JP, Rietschel ET, Tersikh A, Ulmer AJ, Yu K, Zahringer U, Khatri I
International immunopharmacology5069-76(2017 Sep)

UBE2O remodels the proteome during terminal erythroid differentiation.
Nguyen AT, Prado MA, Schmidt PJ, Sendamarai AK, Wilson-Grady JT, Min M, Campagna DR, Tian G, Shi Y, Dederer V, Kawan M, Kuehnle N, Paulo JA, Yao Y, Weiss MJ, Justice MJ, Gygi SP, Fleming MD, Finley D
Science (New York, N.Y.)357(6350)(2017 08)

Conservation of sequence and structure flanking the mouse and human beta-globin loci: the beta-globin genes are embedded within an array of odorant receptor genes.
Bulger M, van Doorninck JH, Saitoh N, Telling A, Farrell C, Bender MA, Felsenfeld G, Axel R, Groudine M, von Doorninck JH
Proceedings of the National Academy of Sciences of the United States of America96(9)5129-34(1999 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A mutation in the mouse Hbb-b2 gene precisely mimics the human hemoglobin Hotel Dieu variant, and results in increased affinity of Hb for oxygen, elevated erythropoietin levels and splenomegaly with excess erythropoiesis.
Title: Thrombocytopenia and erythrocytosis in mice with a mutation in the gene encoding the hemoglobin ? minor chain.

The following Hbb-b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hbb-b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16366	NM_016956.3 Latest version!	Mus musculus hemoglobin, beta adult minor chain (Hbb-b2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hbb-b2 ( NM_016956.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058652.1 Mus musculus hemoglobin, beta adult minor chain (Hbb-b2), mRNA.

Price & Availability↑

CloneID	OMu16366
Clone ID Related Accession (Same CDS sequence)	NM_016956.3
Accession Version	NM_016956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	hemoglobin subunit beta-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X14061.1. On or before Feb 15, 2015 this sequence version replaced XM_011238089.1, XM_006544228.1, NM_016956.2. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the alternate haplotype that this gene represents. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AI036344.1, AK011119.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCACC TGACTGATGC TGAGAAGTCT GCTGTCTCTT GCCTGTGGGC AAAGGTGAAC 
CCCGATGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TTGTCTACCC TTGGACCCAG 
CGGTACTTTG ATAGCTTTGG AGACCTATCC TCTGCCTCTG CTATCATGGG TAATCCCAAG 
GTGAAGGCCC ATGGCAAAAA GGTGATAACT GCCTTTAACG AGGGCCTGAA AAACCTGGAC 
AACCTCAAGG GCACCTTTGC CAGCCTCAGT GAGCTCCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAGGCTCCT AGGCAATGCG ATCGTGATTG TGCTGGGCCA CCACCTGGGC 
AAGGATTTCA CCCCTGCTGC ACAGGCTGCC TTCCAGAAGG TGGTGGCTGG AGTGGCCACT 
GCCCTGGCTC ACAAGTACCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058652.1
CDS	55..498
Translation	MVHLTDAEKSAVSCLWAKVNPDEVGGEALGRLLVVYPWTQRYFDSFGDLSSASAIMGNPKVKAHGKKVITAFNEGLKNLDNLKGTFASLSELHCDKLHVDPENFRLLGNAIVIVLGHHLGKDFTPAAQAAFQKVVAGVATALAHKYH

Target ORF information:

RefSeq Version	NM_016956.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hemoglobin, beta adult minor chain (Hbb-b2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016956.3

ORF Insert Sequence:

ATGGTGCACC TGACTGATGC TGAGAAGTCT GCTGTCTCTT GCCTGTGGGC AAAGGTGAAC 
CCCGATGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TTGTCTACCC TTGGACCCAG 
CGGTACTTTG ATAGCTTTGG AGACCTATCC TCTGCCTCTG CTATCATGGG TAATCCCAAG 
GTGAAGGCCC ATGGCAAAAA GGTGATAACT GCCTTTAACG AGGGCCTGAA AAACCTGGAC 
AACCTCAAGG GCACCTTTGC CAGCCTCAGT GAGCTCCACT GTGACAAGCT GCATGTGGAT 
CCTGAGAACT TCAGGCTCCT AGGCAATGCG ATCGTGATTG TGCTGGGCCA CCACCTGGGC 
AAGGATTTCA CCCCTGCTGC ACAGGCTGCC TTCCAGAAGG TGGTGGCTGG AGTGGCCACT 
GCCCTGGCTC ACAAGTACCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An altered REDOX environment, assisted by over-expression of fetal hemoglobins, protects from inflammatory colitis and reduces inflammatory cytokine expression.
International immunopharmacology5069-76(2017 Sep)
Gorczynski RM,Alexander C,Brandenburg K,Chen Z,Heini A,Neumann D,Mach JP,Rietschel ET,Tersikh A,Ulmer AJ,Yu K,Zahringer U,Khatri I

UBE2O remodels the proteome during terminal erythroid differentiation.
Science (New York, N.Y.)357(6350)(2017 08)
Nguyen AT,Prado MA,Schmidt PJ,Sendamarai AK,Wilson-Grady JT,Min M,Campagna DR,Tian G,Shi Y,Dederer V,Kawan M,Kuehnle N,Paulo JA,Yao Y,Weiss MJ,Justice MJ,Gygi SP,Fleming MD,Finley D

Conservation of sequence and structure flanking the mouse and human beta-globin loci: the beta-globin genes are embedded within an array of odorant receptor genes.
Proceedings of the National Academy of Sciences of the United States of America96(9)5129-34(1999 Apr)
Bulger M,van Doorninck JH,Saitoh N,Telling A,Farrell C,Bender MA,Felsenfeld G,Axel R,Groudine M,von Doorninck JH

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Hbb-b2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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