Hadh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hadh
Entrez Gene ID	15107
Full Name	hydroxyacyl-Coenzyme A dehydrogenase
Synonyms	AA409008,AU019341,AW742602,HCDH,Hadhsc,Schad
General protein information	Preferred Names hydroxyacyl-Coenzyme A dehydrogenase Names hydroxyacyl-coenzyme A dehydrogenase, mitochondrial L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain hydroxylacyl-Coenzyme A dehydrogenase-dehydrogenase medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase short-chain 3-hydroxyacyl-CoA dehydrogenase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 G3

mRNA and Protein(s)

mRNA	Protein	Name
NM_008212.4	NP_032238.2	hydroxyacyl-coenzyme A dehydrogenase, mitochondrial precursor

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	B0272.3	NP_509584.1
Neurospora crassa	NCU09553	XP_958772.1
Mus musculus (house mouse)	Hadh	NP_032238.2
Arabidopsis thaliana (thale cress)	AT3G15290	NP_188147.1
Xenopus tropicalis (tropical clawed frog)	hadh	NP_001011073.1
	HADH	XP_001084834.2
Canis lupus familiaris (dog)	HADH	XP_003434079.1
Bos taurus (cattle)	HADH	NP_001039799.1
Caenorhabditis elegans (roundworm)	F54C8.1	NP_499075.1
Magnaporthe oryzae (rice blast fungus)	MGG_16630	XP_003711327.1
Oryza sativa (rice)	Os01g0796400	NP_001044515.1
Homo sapiens (human)	HADH	NP_001171634.2
Pan troglodytes (chimpanzee)	HADH	XP_517385.2
Gallus gallus (chicken)	HADH	NP_001264826.1
Danio rerio (zebrafish)	hadh	NP_001003515.1
Rattus norvegicus (Norway rat)	Hadh	NP_476534.1

Gene Ontology
Bibliography

Related articles in PubMed

The Hypoglycemic Phenotype Is Islet Cell-Autonomous in Short-Chain Hydroxyacyl-CoA Dehydrogenase-Deficient Mice.
Molven A, Hollister-Lock J, Hu J, Martinez R, Nj?lstad PR, Liew CW, Weir G, Kulkarni RN
Diabetes65(6)1672-8(2016 06)

Nutritional stress exacerbates hepatic steatosis induced by deletion of the histidine nucleotide-binding (Hint2) mitochondrial protein.
Martin J, Balmer ML, Rajendran S, Maurhofer O, Dufour JF, St-Pierre MV
American journal of physiology. Gastrointestinal and liver physiology310(7)G497-509(2016 Apr)

Short-chain 3-hydroxyacyl-coenzyme A dehydrogenase associates with a protein super-complex integrating multiple metabolic pathways.
Narayan SB, Master SR, Sireci AN, Bierl C, Stanley PE, Li C, Stanley CA, Bennett MJ
PloS one7(4)e35048(2012)

Role of medium- and short-chain L-3-hydroxyacyl-CoA dehydrogenase in the regulation of body weight and thermogenesis.
Schulz N, Himmelbauer H, Rath M, van Weeghel M, Houten S, Kulik W, Suhre K, Scherneck S, Vogel H, Kluge R, Wiedmer P, Joost HG, Sch?rmann A
Endocrinology152(12)4641-51(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A physical association between short-chain 3-hydroxyacyl-coenzyme A dehydrogenase and important components of other key metabolic pathways. Most of the interactions were with enzymes in mitochondrial pathways.
Title: Short-chain 3-hydroxyacyl-coenzyme A dehydrogenase associates with a protein super-complex integrating multiple metabolic pathways.

SCHAD is involved in thermogenesis, in the maintenance of body weight, and in the regulation of nutrient-stimulated insulin secretion
Title: Role of medium- and short-chain L-3-hydroxyacyl-CoA dehydrogenase in the regulation of body weight and thermogenesis.

SCHAD deficiency causes hyperinsulinism by activation of GDH via loss of inhibitory regulation of GDH by SCHAD.
Title: Mechanism of hyperinsulinism in short-chain 3-hydroxyacyl-CoA dehydrogenase deficiency involves activation of glutamate dehydrogenase.

SCHAD regulates insulin secretion through a KATP channel-independent mechanism.
Title: Functional genomics of the beta-cell: short-chain 3-hydroxyacyl-coenzyme A dehydrogenase regulates insulin secretion independent of K+ currents.

Results demonstrate that L-3-hydroxyacyl-CoA dehydrogenase type II (HADH II/ABAD) modulates 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine neurotoxicity and suggests that mimetics may provide protective benefit in the treatment of Parkinson disease.
Title: L-3-hydroxyacyl-CoA dehydrogenase II protects in a model of Parkinson's disease.

The following Hadh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hadh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16357	NM_008212.4 Latest version!	Mus musculus hydroxyacyl-Coenzyme A dehydrogenase (Hadh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hadh ( NM_008212.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032238.2 Mus musculus hydroxyacyl-Coenzyme A dehydrogenase (Hadh), mRNA.

Price & Availability↑

CloneID	OMu16357
Clone ID Related Accession (Same CDS sequence)	NM_008212.4
Accession Version	NM_008212.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	hydroxyacyl-coenzyme A dehydrogenase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC123634.10, AK132260.1, AK148486.1 and AI845880.1. On Aug 14, 2013 this sequence version replaced NM_008212.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132260.1, AK148486.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTTCG TGACCAGGCA ATTCTTGCGC TCCATGTCCT CCTCTTCCTC TGCGTCGGCG 
GCCGCCAAGA AGATCCTGAT CAAGCATGTG ACCGTCATCG GCGGCGGGCT GATGGGCGCC 
GGCATCGCGC AGGTAGCTGC AGCAACTGGC CATACAGTAG TATTGGTGGA CCAAACGGAA 
GACATCCTGG CAAAATCCAA GAAGGGAATT GAGGAGAGTC TAAAGAGGAT GGCAAAGAAG 
AAGTTCACAG AAAACCCTAA GGCTGGAGAT GAGTTTGTGG AGAAGACCCT GAGCTGCCTT 
TCAACCAGCA CCGATGCGGC ATCCGTGGTG CACAGCACAG ACCTGGTGGT GGAGGCCATT 
GTGGAGAACC TGAAGCTGAA GAACGAGCTG TTCCAGAGGC TGGACAAGTT CGCTGCAGAA 
CACACCATCT TTGCCAGCAA CACGTCTTCT TTGCAGATCA CAAACATAGC CAATGCCACC 
ACCAGACAAG ACCGATTTGC TGGCCTCCAC TTTTTCAACC CCGTGCCTAT GATGAAGCTT 
GTGGAGGTCA TCAAAACACC GATGACCAGC CAGAAGACAT TTGAATCTCT GGTGGACTTT 
TGTAAAACCT TAGGAAAGCA TCCTGTCTCT TGCAAGGACA CTCCTGGATT CATCGTGAAC 
CGACTCTTGG TGCCATACCT CATTGAGGCC GTCAGGCTAC ACGAGCGAGG CGATGCGTCT 
AAGGAAGACA TCGATACAGC GATGAAGCTG GGAGCTGGGT ATCCCATGGG TCCGTTTGAG 
CTTCTTGACT ATGTTGGACT GGATACTACA AAGTTCATCT TGGACGGGTG GCATGAAATG 
GAGCCAGAGA ACCCCTTATT TCAGCCCAGT CCTTCCATGA ATAATCTGGT GGCCCAGAAG 
AAGCTGGGCA AGAAGACCGG AGAAGGATTT TACAAATACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032238.2
CDS	95..1039
Translation	MAFVTRQFLRSMSSSSSASAAAKKILIKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGIEESLKRMAKKKFTENPKAGDEFVEKTLSCLSTSTDAASVVHSTDLVVEAIVENLKLKNELFQRLDKFAAEHTIFASNTSSLQITNIANATTRQDRFAGLHFFNPVPMMKLVEVIKTPMTSQKTFESLVDFCKTLGKHPVSCKDTPGFIVNRLLVPYLIEAVRLHERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFILDGWHEMEPENPLFQPSPSMNNLVAQKKLGKKTGEGFYKYK

Target ORF information:

RefSeq Version	NM_008212.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxyacyl-Coenzyme A dehydrogenase (Hadh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008212.4

ORF Insert Sequence:

ATGGCGTTCG TGACCAGGCA ATTCTTGCGC TCCATGTCCT CCTCTTCCTC TGCGTCGGCG 
GCCGCCAAGA AGATCCTGAT CAAGCATGTG ACCGTCATCG GCGGCGGGCT GATGGGCGCC 
GGCATCGCGC AGGTAGCTGC AGCAACTGGC CATACAGTAG TATTGGTGGA CCAAACGGAA 
GACATCCTGG CAAAATCCAA GAAGGGAATT GAGGAGAGTC TAAAGAGGAT GGCAAAGAAG 
AAGTTCACAG AAAACCCTAA GGCTGGAGAT GAGTTTGTGG AGAAGACCCT GAGCTGCCTT 
TCAACCAGCA CCGATGCGGC ATCCGTGGTG CACAGCACAG ACCTGGTGGT GGAGGCCATT 
GTGGAGAACC TGAAGCTGAA GAACGAGCTG TTCCAGAGGC TGGACAAGTT CGCTGCAGAA 
CACACCATCT TTGCCAGCAA CACGTCTTCT TTGCAGATCA CAAACATAGC CAATGCCACC 
ACCAGACAAG ACCGATTTGC TGGCCTCCAC TTTTTCAACC CCGTGCCTAT GATGAAGCTT 
GTGGAGGTCA TCAAAACACC GATGACCAGC CAGAAGACAT TTGAATCTCT GGTGGACTTT 
TGTAAAACCT TAGGAAAGCA TCCTGTCTCT TGCAAGGACA CTCCTGGATT CATCGTGAAC 
CGACTCTTGG TGCCATACCT CATTGAGGCC GTCAGGCTAC ACGAGCGAGG CGATGCGTCT 
AAGGAAGACA TCGATACAGC GATGAAGCTG GGAGCTGGGT ATCCCATGGG TCCGTTTGAG 
CTTCTTGACT ATGTTGGACT GGATACTACA AAGTTCATCT TGGACGGGTG GCATGAAATG 
GAGCCAGAGA ACCCCTTATT TCAGCCCAGT CCTTCCATGA ATAATCTGGT GGCCCAGAAG 
AAGCTGGGCA AGAAGACCGG AGAAGGATTT TACAAATACA AGTGA