Gzmb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gzmb
Entrez Gene ID	14939
Full Name	granzyme B
Synonyms	AI553453,CCP-1/C11,CCP1,Ctla-1,Ctla1,GZB
General protein information	Preferred Names granzyme B Names granzyme B(G,H) cytotoxic cell protease 1 fragmentin-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 C3\|14 28.19 cM
Summary	This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Mice lacking a functional copy of this gene exhibit impaired immune cell-mediated cytolysis. [provided by RefSeq, Sep 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013542.2	NP_038570.1	granzyme B(G,H) precursor
NM_013542.3	NP_038570.1	granzyme B(G,H) preproprotein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Granzyme B Disrupts Central Metabolism and Protein Synthesis in Bacteria to Promote an Immune Cell Death Program.
Dotiwala F, Sen Santara S, Binker-Cosen AA, Li B, Chandrasekaran S, Lieberman J
Cell171(5)1125-1137.e11(2017 Nov)

The Transcription Factor Tcf1 Contributes to Normal NK Cell Development and Function by Limiting the Expression of Granzymes.
Jeevan-Raj B, Gehrig J, Charmoy M, Chennupati V, Grandcl?ment C, Angelino P, Delorenzi M, Held W
Cell reports20(3)613-626(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Differential proteomics were used to identify granzyme B substrates in three unrelated bacteria: Escherichia coli, Listeria monocytogenes, and Mycobacteria tuberculosis. Granzyme B cleaves a highly conserved set of proteins in all three bacteria, which function in vital biosynthetic and metabolic pathways that are critical for bacterial survival under diverse environmental conditions.
Title: Granzyme B Disrupts Central Metabolism and Protein Synthesis in Bacteria to Promote an Immune Cell Death Program.

serglycin plays a critical role in the maturation of dense-core cytotoxic granules in cytotoxic lymphocytes and the trafficking and storage of perforin and granzyme B, whereas granzyme A is unaffected
Title: Serglycin determines secretory granule repertoire and regulates natural killer cell and cytotoxic T lymphocyte cytotoxicity.

these results suggest a perforin-independent, extracellular role for GzmB in the pathogenesis of cardiac fibrosis
Title: Granzyme B Deficiency Protects against Angiotensin II-Induced Cardiac Fibrosis.

this study shows that gzmA and gzmB partly regulate local inflammation during early pneumonia but eventually play an insignificant role during pneumosepsis by the common human pathogen Klebsiella pneumoniae
Title: Granzymes A and B Regulate the Local Inflammatory Response during Klebsiella pneumoniae Pneumonia.

data indicate an involvement of granzyme B in the neuroinflammation mediated by Eomes(+) CD4(+) T cells.
Title: Eomesodermin-expressing T-helper cells are essential for chronic neuroinflammation.

The following Gzmb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gzmb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19345	NM_013542.3 Latest version!	Mus musculus granzyme B (Gzmb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OMu19345	NM_013542.2	Mus musculus granzyme B (Gzmb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gzmb ( NM_013542.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038570.1 Mus musculus granzyme B (Gzmb), mRNA.

Price & Availability↑

CloneID	OMu19345
Clone ID Related Accession (Same CDS sequence)	NM_013542.2 , NM_013542.3
Accession Version	NM_013542.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	granzyme B(G,H) precursor
Comment

ATGAAGATCC TCCTGCTACT GCTGACCTTG TCTCTGGCCT CCAGGACAAA GGCAGGGGAG 
ATCATCGGGG GACATGAAGT CAAGCCCCAC TCTCGACCCT ACATGGCCTT ACTTTCGATC 
AAGGATCAGC AGCCTGAGGC GATATGTGGG GGCTTCCTTA TTCGAGAGGA CTTTGTGCTG 
ACTGCTGCTC ACTGTGAAGG AAGTATAATA AATGTCACTT TGGGGGCCCA CAACATCAAA 
GAACAGGAGA AGACCCAGCA AGTCATCCCT ATGGTAAAAT GCATTCCCCA CCCAGACTAT 
AATCCTAAGA CATTCTCCAA TGACATCATG CTGCTAAAGC TGAAGAGTAA GGCCAAGAGG 
ACTAGAGCTG TGAGGCCCCT CAACCTGCCC AGGCGCAATG TCAATGTGAA GCCAGGAGAT 
GTGTGCTATG TGGCTGGTTG GGGAAGGATG GCCCCAATGG GCAAATACTC AAACACGCTA 
CAAGAGGTTG AGCTGACAGT ACAGAAGGAT CGGGAGTGTG AGTCCTACTT TAAAAATCGT 
TACAACAAAA CCAATCAGAT ATGTGCGGGG GACCCAAAGA CCAAACGTGC TTCCTTTCGG 
GGGGATTCTG GAGGCCCGCT TGTGTGTAAA AAAGTGGCTG CAGGCATAGT TTCCTATGGA 
TATAAGGATG GTTCACCTCC ACGTGCTTTC ACCAAAGTCT CGAGTTTCTT ATCCTGGATA 
AAGAAAACAA TGAAAAGCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038570.1
Translation	MKILLLLLTLSLASRTKAGEIIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS

Target ORF information:

RefSeq Version	NM_013542.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus granzyme B (Gzmb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013542.2

ORF Insert Sequence:

ATGAAGATCC TCCTGCTACT GCTGACCTTG TCTCTGGCCT CCAGGACAAA GGCAGGGGAG 
ATCATCGGGG GACATGAAGT CAAGCCCCAC TCTCGACCCT ACATGGCCTT ACTTTCGATC 
AAGGATCAGC AGCCTGAGGC GATATGTGGG GGCTTCCTTA TTCGAGAGGA CTTTGTGCTG 
ACTGCTGCTC ACTGTGAAGG AAGTATAATA AATGTCACTT TGGGGGCCCA CAACATCAAA 
GAACAGGAGA AGACCCAGCA AGTCATCCCT ATGGTAAAAT GCATTCCCCA CCCAGACTAT 
AATCCTAAGA CATTCTCCAA TGACATCATG CTGCTAAAGC TGAAGAGTAA GGCCAAGAGG 
ACTAGAGCTG TGAGGCCCCT CAACCTGCCC AGGCGCAATG TCAATGTGAA GCCAGGAGAT 
GTGTGCTATG TGGCTGGTTG GGGAAGGATG GCCCCAATGG GCAAATACTC AAACACGCTA 
CAAGAGGTTG AGCTGACAGT ACAGAAGGAT CGGGAGTGTG AGTCCTACTT TAAAAATCGT 
TACAACAAAA CCAATCAGAT ATGTGCGGGG GACCCAAAGA CCAAACGTGC TTCCTTTCGG 
GGGGATTCTG GAGGCCCGCT TGTGTGTAAA AAAGTGGCTG CAGGCATAGT TTCCTATGGA 
TATAAGGATG GTTCACCTCC ACGTGCTTTC ACCAAAGTCT CGAGTTTCTT ATCCTGGATA 
AAGAAAACAA TGAAAAGCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gzmb ( NM_013542.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038570.1 Mus musculus granzyme B (Gzmb), mRNA.

Price & Availability↑

CloneID	OMu19345
Clone ID Related Accession (Same CDS sequence)	NM_013542.2 , NM_013542.3
Accession Version	NM_013542.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Mus musculus(house mouse)
Product	granzyme B(G,H) preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC091783.9, BY227723.1, AK157856.1 and BU758224.1. On Sep 20, 2016 this sequence version replaced NM_013542.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002085.1, X04072.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGATCC TCCTGCTACT GCTGACCTTG TCTCTGGCCT CCAGGACAAA GGCAGGGGAG 
ATCATCGGGG GACATGAAGT CAAGCCCCAC TCTCGACCCT ACATGGCCTT ACTTTCGATC 
AAGGATCAGC AGCCTGAGGC GATATGTGGG GGCTTCCTTA TTCGAGAGGA CTTTGTGCTG 
ACTGCTGCTC ACTGTGAAGG AAGTATAATA AATGTCACTT TGGGGGCCCA CAACATCAAA 
GAACAGGAGA AGACCCAGCA AGTCATCCCT ATGGTAAAAT GCATTCCCCA CCCAGACTAT 
AATCCTAAGA CATTCTCCAA TGACATCATG CTGCTAAAGC TGAAGAGTAA GGCCAAGAGG 
ACTAGAGCTG TGAGGCCCCT CAACCTGCCC AGGCGCAATG TCAATGTGAA GCCAGGAGAT 
GTGTGCTATG TGGCTGGTTG GGGAAGGATG GCCCCAATGG GCAAATACTC AAACACGCTA 
CAAGAGGTTG AGCTGACAGT ACAGAAGGAT CGGGAGTGTG AGTCCTACTT TAAAAATCGT 
TACAACAAAA CCAATCAGAT ATGTGCGGGG GACCCAAAGA CCAAACGTGC TTCCTTTCGG 
GGGGATTCTG GAGGCCCGCT TGTGTGTAAA AAAGTGGCTG CAGGCATAGT TTCCTATGGA 
TATAAGGATG GTTCACCTCC ACGTGCTTTC ACCAAAGTCT CGAGTTTCTT ATCCTGGATA 
AAGAAAACAA TGAAAAGCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038570.1
CDS	186..929
Translation	MKILLLLLTLSLASRTKAGEIIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS

Target ORF information:

RefSeq Version	NM_013542.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus granzyme B (Gzmb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013542.3

ORF Insert Sequence:

ATGAAGATCC TCCTGCTACT GCTGACCTTG TCTCTGGCCT CCAGGACAAA GGCAGGGGAG 
ATCATCGGGG GACATGAAGT CAAGCCCCAC TCTCGACCCT ACATGGCCTT ACTTTCGATC 
AAGGATCAGC AGCCTGAGGC GATATGTGGG GGCTTCCTTA TTCGAGAGGA CTTTGTGCTG 
ACTGCTGCTC ACTGTGAAGG AAGTATAATA AATGTCACTT TGGGGGCCCA CAACATCAAA 
GAACAGGAGA AGACCCAGCA AGTCATCCCT ATGGTAAAAT GCATTCCCCA CCCAGACTAT 
AATCCTAAGA CATTCTCCAA TGACATCATG CTGCTAAAGC TGAAGAGTAA GGCCAAGAGG 
ACTAGAGCTG TGAGGCCCCT CAACCTGCCC AGGCGCAATG TCAATGTGAA GCCAGGAGAT 
GTGTGCTATG TGGCTGGTTG GGGAAGGATG GCCCCAATGG GCAAATACTC AAACACGCTA 
CAAGAGGTTG AGCTGACAGT ACAGAAGGAT CGGGAGTGTG AGTCCTACTT TAAAAATCGT 
TACAACAAAA CCAATCAGAT ATGTGCGGGG GACCCAAAGA CCAAACGTGC TTCCTTTCGG 
GGGGATTCTG GAGGCCCGCT TGTGTGTAAA AAAGTGGCTG CAGGCATAGT TTCCTATGGA 
TATAAGGATG GTTCACCTCC ACGTGCTTTC ACCAAAGTCT CGAGTTTCTT ATCCTGGATA 
AAGAAAACAA TGAAAAGCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Granzyme B Disrupts Central Metabolism and Protein Synthesis in Bacteria to Promote an Immune Cell Death Program.
Cell171(5)1125-1137.e11(2017 Nov)
Dotiwala F,Sen Santara S,Binker-Cosen AA,Li B,Chandrasekaran S,Lieberman J

The Transcription Factor Tcf1 Contributes to Normal NK Cell Development and Function by Limiting the Expression of Granzymes.
Cell reports20(3)613-626(2017 Jul)
Jeevan-Raj B,Gehrig J,Charmoy M,Chennupati V,Grandcl?ment C,Angelino P,Delorenzi M,Held W

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Gzmb cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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