Trip12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trip12
Entrez Gene ID	14897
Full Name	thyroid hormone receptor interactor 12
Synonyms	1110036I07Rik,6720416K24Rik,AA410158,Gtl6,TRIP-12
General protein information	Preferred Names thyroid hormone receptor interactor 12 Names E3 ubiquitin-protein ligase TRIP12 HECT-type E3 ubiquitin transferase TRIP12 TR-interacting protein 12 gene trap locus 6 thyroid receptor interactor TRIP12 thyroid receptor-interacting protein 12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C5

mRNA and Protein(s)

mRNA	Protein	Name
XM_011238655.2	XP_011236957.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_017316494.1	XP_017171983.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011238661.2	XP_011236963.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_011238671.2	XP_011236973.1	E3 ubiquitin-protein ligase TRIP12 isoform X15
XM_011238672.2	XP_011236974.1	E3 ubiquitin-protein ligase TRIP12 isoform X15
XM_011238673.2	XP_011236975.1	E3 ubiquitin-protein ligase TRIP12 isoform X16
XM_030248895.1	XP_030104755.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_017316627.2	XP_017172116.1	E3 ubiquitin-protein ligase TRIP12 isoform X14
XM_017316673.2	XP_017172162.1	E3 ubiquitin-protein ligase TRIP12 isoform X19
XM_011238669.3	XP_011236971.1	E3 ubiquitin-protein ligase TRIP12 isoform X21
XM_030249195.1	XP_030105055.1	E3 ubiquitin-protein ligase TRIP12 isoform X22
XM_030249293.1	XP_030105153.1	E3 ubiquitin-protein ligase TRIP12 isoform X28
XM_030249310.1	XP_030105170.1	E3 ubiquitin-protein ligase TRIP12 isoform X29
XM_011238654.2	XP_011236956.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011238658.2	XP_011236960.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011238665.2	XP_011236967.1	E3 ubiquitin-protein ligase TRIP12 isoform X9
XM_011238666.2	XP_011236968.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_011238669.2	XP_011236971.1	E3 ubiquitin-protein ligase TRIP12 isoform X15
XM_006496429.2	XP_006496492.1	E3 ubiquitin-protein ligase TRIP12 isoform X19
NM_133975.4	NP_598736.4	E3 ubiquitin-protein ligase TRIP12
XM_011238654.3	XP_011236956.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011238655.3	XP_011236957.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011238657.3	XP_011236959.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011238659.2	XP_011236961.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011238660.3	XP_011236962.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011238663.3	XP_011236965.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_011238665.3	XP_011236967.1	E3 ubiquitin-protein ligase TRIP12 isoform X12
XM_011238667.3	XP_011236969.1	E3 ubiquitin-protein ligase TRIP12 isoform X15
XM_030248998.1	XP_030104858.1	E3 ubiquitin-protein ligase TRIP12 isoform X16
XM_011238670.3	XP_011236972.1	E3 ubiquitin-protein ligase TRIP12 isoform X21
XM_011238671.3	XP_011236973.1	E3 ubiquitin-protein ligase TRIP12 isoform X21
XM_011238673.3	XP_011236975.1	E3 ubiquitin-protein ligase TRIP12 isoform X22
XM_006496429.3	XP_006496492.1	E3 ubiquitin-protein ligase TRIP12 isoform X32
XM_006496416.3	XP_006496479.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_017316627.1	XP_017172116.1	E3 ubiquitin-protein ligase TRIP12 isoform X11
XM_017316673.1	XP_017172162.1	E3 ubiquitin-protein ligase TRIP12 isoform X14
XM_011238662.2	XP_011236964.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_011238662.3	XP_011236964.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_011238664.3	XP_011236966.1	E3 ubiquitin-protein ligase TRIP12 isoform X9
XM_011238666.3	XP_011236968.1	E3 ubiquitin-protein ligase TRIP12 isoform X13
XM_030249012.1	XP_030104872.1	E3 ubiquitin-protein ligase TRIP12 isoform X17
XM_030249060.1	XP_030104920.1	E3 ubiquitin-protein ligase TRIP12 isoform X20
XM_030249149.1	XP_030105009.1	E3 ubiquitin-protein ligase TRIP12 isoform X21
XM_006496427.3	XP_006496490.1	E3 ubiquitin-protein ligase TRIP12 isoform X23
XM_030249285.1	XP_030105145.1	E3 ubiquitin-protein ligase TRIP12 isoform X27
XM_011238657.2	XP_011236959.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011238660.2	XP_011236962.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_006496414.3	XP_006496477.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_011238663.2	XP_011236965.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_006496422.3	XP_006496485.1	E3 ubiquitin-protein ligase TRIP12 isoform X13
XM_011238668.2	XP_011236970.1	E3 ubiquitin-protein ligase TRIP12 isoform X15
XM_006496427.2	XP_006496490.1	E3 ubiquitin-protein ligase TRIP12 isoform X17
XM_011238658.3	XP_011236960.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_011238661.3	XP_011236963.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_006496414.4	XP_006496477.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_030248922.1	XP_030104782.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_030248942.1	XP_030104802.1	E3 ubiquitin-protein ligase TRIP12 isoform X11
XM_006496422.4	XP_006496485.1	E3 ubiquitin-protein ligase TRIP12 isoform X18
XM_011238672.3	XP_011236974.1	E3 ubiquitin-protein ligase TRIP12 isoform X21
XM_030249168.1	XP_030105028.1	E3 ubiquitin-protein ligase TRIP12 isoform X22
XM_030249180.1	XP_030105040.1	E3 ubiquitin-protein ligase TRIP12 isoform X22
XM_011238656.2	XP_011236958.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_006496409.3	XP_006496472.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_011238664.2	XP_011236966.1	E3 ubiquitin-protein ligase TRIP12 isoform X8
XM_011238667.2	XP_011236969.1	E3 ubiquitin-protein ligase TRIP12 isoform X12
XM_011238670.2	XP_011236972.1	E3 ubiquitin-protein ligase TRIP12 isoform X15
XM_011238656.3	XP_011236958.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_030248874.1	XP_030104734.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_006496409.4	XP_006496472.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_006496416.4	XP_006496479.1	E3 ubiquitin-protein ligase TRIP12 isoform X8
XM_011238668.3	XP_011236970.1	E3 ubiquitin-protein ligase TRIP12 isoform X21
XM_030249155.1	XP_030105015.1	E3 ubiquitin-protein ligase TRIP12 isoform X21
XM_030249247.1	XP_030105107.1	E3 ubiquitin-protein ligase TRIP12 isoform X24
XM_030249259.1	XP_030105119.1	E3 ubiquitin-protein ligase TRIP12 isoform X25
XM_030249267.1	XP_030105127.1	E3 ubiquitin-protein ligase TRIP12 isoform X26
XM_030249325.1	XP_030105185.1	E3 ubiquitin-protein ligase TRIP12 isoform X30
XM_011238674.2	XP_011236976.1	E3 ubiquitin-protein ligase TRIP12 isoform X31

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001296	XP_321851.5
	TRIP12	XP_002799130.1
Mus musculus (house mouse)	Trip12	NP_598736.4
Xenopus tropicalis (tropical clawed frog)	trip12	NP_001136384.1
Homo sapiens (human)	TRIP12	NP_004229.1
Canis lupus familiaris (dog)	TRIP12	XP_005635860.1
Gallus gallus (chicken)	TRIP12	XP_004937121.1
Pan troglodytes (chimpanzee)	TRIP12	XP_001138950.1
Bos taurus (cattle)	TRIP12	NP_001178132.1
Arabidopsis thaliana (thale cress)	KAK	NP_849567.2
Rattus norvegicus (Norway rat)	Trip12	NP_001026829.1
Danio rerio (zebrafish)	trip12	XP_005159284.1
Oryza sativa (rice)	Os02g0101700	NP_001045591.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential effects on ARF stability by normal versus oncogenic levels of c-Myc expression.
Chen D, Kon N, Zhong J, Zhang P, Yu L, Gu W
Molecular cell51(1)46-56(2013 Jul)

TRADD contributes to tumour suppression by regulating ULF-dependent p19Arf ubiquitylation.
Chio II, Sasaki M, Ghazarian D, Moreno J, Done S, Ueda T, Inoue S, Chang YL, Chen NJ, Mak TW
Nature cell biology14(6)625-33(2012 May)

The E3 ubiquitin ligase activity of Trip12 is essential for mouse embryogenesis.
Kajiro M, Tsuchiya M, Kawabe Y, Furumai R, Iwasaki N, Hayashi Y, Katano M, Nakajima Y, Goto N, Watanabe T, Murayama A, Oishi H, Ema M, Takahashi S, Kishimoto H, Yanagisawa J
PloS one6(10)e25871(2011)

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Evsikov AV, Graber JH, Brockman JM, Hampl A, Holbrook AE, Singh P, Eppig JJ, Solter D, Knowles BB
Genes & development20(19)2713-27(2006 Oct)

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data indicate that TRADD shuttles dynamically from the cytoplasm into the nucleus to modulate the interaction between p19(Arf) and its E3 ubiquitin ligase ULF, thereby promoting p19(Arf) protein stability and tumour suppression
Title: TRADD contributes to tumour suppression by regulating ULF-dependent p19Arf ubiquitylation.

Trip12 is involved in global gene expression and plays an important role in mouse development.
Title: The E3 ubiquitin ligase activity of Trip12 is essential for mouse embryogenesis.

The following Trip12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trip12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu27722	XM_006496429.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X48, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu85022	XM_030249325.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X46, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu67894	XM_011238674.2 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X47, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu27709	XM_006496409.4 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu27710	XM_006496414.4 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu27712	XM_006496416.4 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu27718	XM_006496422.4 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu27720	XM_006496427.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67884	XM_011238654.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67884	XM_011238656.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67884	XM_011238658.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67884	XM_011238660.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67884	XM_011238655.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67884	XM_011238657.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67884	XM_030248874.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67885	XM_011238661.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67886	XM_011238662.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67887	XM_011238663.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67888	XM_011238664.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67889	XM_011238665.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67890	XM_011238666.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67891	XM_011238667.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67892	XM_030249149.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67892	XM_011238668.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67892	XM_011238671.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67892	XM_030249155.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67892	XM_011238672.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67892	XM_011238670.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67892	XM_011238669.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67893	XM_011238673.3 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67893	XM_030249195.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67893	XM_030249168.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67893	XM_030249180.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu76980	XM_017316627.2 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu76981	XM_017316673.2 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85010	XM_030248895.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85011	XM_030248922.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85012	XM_030248942.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85013	XM_030248998.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85014	XM_030249012.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85015	XM_030249060.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85016	XM_030249247.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85017	XM_030249259.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85018	XM_030249267.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85019	XM_030249285.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X43, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85020	XM_030249293.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X44, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85021	XM_030249310.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X45, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu15102	NM_133975.4 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1490.30 $2129.00
OMu67884	XM_017316494.1 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67884	XM_011238659.2 Latest version!	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu67892	XM_011238669.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67884	XM_011238654.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67884	XM_011238655.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67889	XM_011238665.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67892	XM_011238671.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67893	XM_011238673.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu27722	XM_006496429.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu76980	XM_017316627.1	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu76981	XM_017316673.1	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67886	XM_011238662.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67890	XM_011238666.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67884	XM_011238657.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67884	XM_011238660.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67887	XM_011238663.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu27720	XM_006496427.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67884	XM_011238658.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67885	XM_011238661.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu27710	XM_006496414.3	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu27718	XM_006496422.3	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67892	XM_011238672.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67888	XM_011238664.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67891	XM_011238667.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67892	XM_011238670.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67884	XM_011238656.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu27709	XM_006496409.3	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu27712	XM_006496416.3	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67892	XM_011238668.2	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trip12 ( XM_006496429.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496492.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X48, mRNA.

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CloneID	OMu27722
Clone ID Related Accession (Same CDS sequence)	XM_006496429.2 , XM_006496429.3
Accession Version	XM_006496429.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AAGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA TCCCTTAGCC AATTCTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTAATG AAAGAAGATC CGGAACTGGC TAAGTCTTTC 
ATTAAGACCT TGTTTGGTGT TCTCTATGAA GTGTACAGCT CCTCAGCAGG CCCTGCGGTC 
AGACACAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCTGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCAATGCT ATCCAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCTCTTCAG ATGGCAGAAA TCCTAATGCA AAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGGAGAGAA GGTGTGATGC ATCAAGTCAA ACATTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCCCCC AAGGCATGTA CAAATGGGTC AGGATCCCTG 
GGATCTACAA CACCAGCCAG CAGTGGGACT GCAACGGCAG CAACCAACGC CTCTGCTGAT 
CTGGGGTCAC CTAGCTTGCA GCATAGCAGA GATGATTCTT TGGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTCAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA 
TATTCCCCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCCTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA TATCGAACCA GCACGAACTG CAGGAGTTAG TGGTCTTGCC 
AGGGCAGCCT CAAAAGACAC CATATCCAAT AATAGAGAAA AAATTAAAGG CTGGATTAAG 
GAGCAGGCAC ATAAATTTGT TGAGCGTTAT TTTAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCCTTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCT 
TCATTTGAAA TCCAACATAG TGGGTTTGTG AAACAACTGT TGCTGTATTT GACATCTAAG 
AATGAAAAGG ATGCTGTCGG CAGAGAGATC CGGTTAAAAC GTTTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT GGAAGAGTAG AACCAGTGGG CCATGCACCT 
TTGCTGGCAT TGGTTCACAA GATGAACAAC TGTCTCAGCC AAATGGAGCA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGGAACGGA GCTGGAGGAA GCTTTTCTCT CAACAGAGGA 
TCACAAGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GTCAGTTACA AAGACACCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAAGGC GGGCCTGTCA AGATTGATCC CCTGGCTCTG 
GTGCAAGCTA TTGAGAGATA CCTTGTGGTT AGAGGATATG GAAGAGTAAG AGAAGATGAT 
GAAGATAGTG ATGATGATGG GTCAGATGAA GAAATAGATG AGTCTTTGGC TGCTCAATTC 
TTAAATTCGG GAAATGTAAG GCACAGGCTA CAGTTTTATA TTGGAGAACA TTTACTACCA 
TATAACATGA CCGTGTATCA GGCAGTGCGG CAATTCAGTG TACAGGCTGA AGATGAAAGA 
GAATCTACTG ATGATGAGAG CAATCCTCTG GGAAGAGCTG GAATTTGGAC AAAGACTCAT 
ACAATATGGT ACAAACCTGT GAGGGAGGAT GAAGAAAGTA CTAAAGATTG TGTTGGTGGC 
AAAAGGGGGA GAGCCCAAAC AGCTCCAACA AAAACTTCCC CCAGAAATGC AAAGAAGCAT 
GATGAGTTAT GGCATGATGG AGTGTGCCCG TCAGTAGCAA ATCCTTTAGA AGTTTACCTC 
ATTCCCACAC CACCTGAAAA TATCACCTTT GAAGACCCAT CCTTAGATGT AATACTTCTT 
TTAAGAGTTT TACATGCCAT CAGTCGATAT TGGTATTACT TGTATGATAA TGCGATGTGC 
AAGGAGATTA TTCCAACTAG TGAATTTATT AACAGTAAAT TAACAGCAAA AGCAAATAGG 
CAGCTTCAAG ACCCTCTAGT AATCATGACA GGAAACATCC CAACATGGCT CACTGAGCTA 
GGAAAAACCT GCCCATTTTT CTTTCCTTTT GATACCCGAC AAATGCTTTT TTATGTAACT 
GCATTTGATC GGGACCGGGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCTGATTCTC AAGACAGCAG AGTTGCGCCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TACTGAAACA AGCTGAGTCT GTGATGCAGG ACCTTGGCAG CTCTCGGGCC 
ATGTTAGAAA TCCAATATGA AAATGAGGTT GGTACAGGTC TGGGGCCAAC ACTGGAGTTT 
TATGCACTTG TTTCTCAGGA ACTACAGAGA GCTGACTTGT GTCTCTGGAG GGGTGAAGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CGCTTCCCTT TGGTAGGACA GCTAAGCCAG CTCACATCGC AAAGGTTAAG 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCTAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCTC TTGGCCTGCC CTTTTATAAG TGGATGCTGC GGCAGGAAAC TTCATTGACA 
TCTCATGATT TATTTGACAT TGATCCTGTT GTAGCCAGAT CAGTATATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTGGAGCAG GATAAATCCC AGACCAAAGA GAGTCTACAA 
TATGCATTGG AAACTCTTAC GATGAATGGC TGTTCAGTGG AAGATCTGGG ATTGGATTTT 
ACGCTGCCTG GGTTTCCCAA CATTGAATTG AAGAAAGGAG GGAAGGATAT TCCAGTTACT 
ATCCACAATT TGGAAGAGTA CCTGAGATTG GTGATATTCT GGGCACTAAA TGAAGGTGTT 
TGTCGACAGT TTGACTCATT CAGAGATGGA TTTGAATCGG TCTTCCCGCT CTGCCATCTT 
CAGTACTTCT ACCCAGAAGA GCTGGACCAG CTCCTGTGTG GCAGTAAAGC AGACACTTGG 
GATGCAAAGA CATTGATGGA ATGCTGCAGG CCTGATCATG GCTATACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTGTTT 
CTCCAGTTTG TGACTGGTAG CCCACGATTG CCAGTTGGAG GATTTCGGAG TTTAAATCCA 
CCTCTGACAA TTGTGCGGAA GACATTTGAA TCAACAGAAA ACCCAGATGA CTTCTTACCA 
TCAGTAATGA CTTGTGTGAA CTATCTTAAA TTGCCGGACT ACTCGAGCAT TGACATAATG 
CGGGACAAAC TCTTGATAGC AGCAAGAGAA GGCCAACAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496492.1
CDS	363..6359
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496429.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X48, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496429.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AAGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA TCCCTTAGCC AATTCTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTAATG AAAGAAGATC CGGAACTGGC TAAGTCTTTC 
ATTAAGACCT TGTTTGGTGT TCTCTATGAA GTGTACAGCT CCTCAGCAGG CCCTGCGGTC 
AGACACAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCTGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCAATGCT ATCCAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCTCTTCAG ATGGCAGAAA TCCTAATGCA AAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGGAGAGAA GGTGTGATGC ATCAAGTCAA ACATTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCCCCC AAGGCATGTA CAAATGGGTC AGGATCCCTG 
GGATCTACAA CACCAGCCAG CAGTGGGACT GCAACGGCAG CAACCAACGC CTCTGCTGAT 
CTGGGGTCAC CTAGCTTGCA GCATAGCAGA GATGATTCTT TGGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTCAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA 
TATTCCCCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCCTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA TATCGAACCA GCACGAACTG CAGGAGTTAG TGGTCTTGCC 
AGGGCAGCCT CAAAAGACAC CATATCCAAT AATAGAGAAA AAATTAAAGG CTGGATTAAG 
GAGCAGGCAC ATAAATTTGT TGAGCGTTAT TTTAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCCTTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCT 
TCATTTGAAA TCCAACATAG TGGGTTTGTG AAACAACTGT TGCTGTATTT GACATCTAAG 
AATGAAAAGG ATGCTGTCGG CAGAGAGATC CGGTTAAAAC GTTTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT GGAAGAGTAG AACCAGTGGG CCATGCACCT 
TTGCTGGCAT TGGTTCACAA GATGAACAAC TGTCTCAGCC AAATGGAGCA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGGAACGGA GCTGGAGGAA GCTTTTCTCT CAACAGAGGA 
TCACAAGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GTCAGTTACA AAGACACCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAAGGC GGGCCTGTCA AGATTGATCC CCTGGCTCTG 
GTGCAAGCTA TTGAGAGATA CCTTGTGGTT AGAGGATATG GAAGAGTAAG AGAAGATGAT 
GAAGATAGTG ATGATGATGG GTCAGATGAA GAAATAGATG AGTCTTTGGC TGCTCAATTC 
TTAAATTCGG GAAATGTAAG GCACAGGCTA CAGTTTTATA TTGGAGAACA TTTACTACCA 
TATAACATGA CCGTGTATCA GGCAGTGCGG CAATTCAGTG TACAGGCTGA AGATGAAAGA 
GAATCTACTG ATGATGAGAG CAATCCTCTG GGAAGAGCTG GAATTTGGAC AAAGACTCAT 
ACAATATGGT ACAAACCTGT GAGGGAGGAT GAAGAAAGTA CTAAAGATTG TGTTGGTGGC 
AAAAGGGGGA GAGCCCAAAC AGCTCCAACA AAAACTTCCC CCAGAAATGC AAAGAAGCAT 
GATGAGTTAT GGCATGATGG AGTGTGCCCG TCAGTAGCAA ATCCTTTAGA AGTTTACCTC 
ATTCCCACAC CACCTGAAAA TATCACCTTT GAAGACCCAT CCTTAGATGT AATACTTCTT 
TTAAGAGTTT TACATGCCAT CAGTCGATAT TGGTATTACT TGTATGATAA TGCGATGTGC 
AAGGAGATTA TTCCAACTAG TGAATTTATT AACAGTAAAT TAACAGCAAA AGCAAATAGG 
CAGCTTCAAG ACCCTCTAGT AATCATGACA GGAAACATCC CAACATGGCT CACTGAGCTA 
GGAAAAACCT GCCCATTTTT CTTTCCTTTT GATACCCGAC AAATGCTTTT TTATGTAACT 
GCATTTGATC GGGACCGGGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCTGATTCTC AAGACAGCAG AGTTGCGCCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TACTGAAACA AGCTGAGTCT GTGATGCAGG ACCTTGGCAG CTCTCGGGCC 
ATGTTAGAAA TCCAATATGA AAATGAGGTT GGTACAGGTC TGGGGCCAAC ACTGGAGTTT 
TATGCACTTG TTTCTCAGGA ACTACAGAGA GCTGACTTGT GTCTCTGGAG GGGTGAAGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CGCTTCCCTT TGGTAGGACA GCTAAGCCAG CTCACATCGC AAAGGTTAAG 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCTAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCTC TTGGCCTGCC CTTTTATAAG TGGATGCTGC GGCAGGAAAC TTCATTGACA 
TCTCATGATT TATTTGACAT TGATCCTGTT GTAGCCAGAT CAGTATATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTGGAGCAG GATAAATCCC AGACCAAAGA GAGTCTACAA 
TATGCATTGG AAACTCTTAC GATGAATGGC TGTTCAGTGG AAGATCTGGG ATTGGATTTT 
ACGCTGCCTG GGTTTCCCAA CATTGAATTG AAGAAAGGAG GGAAGGATAT TCCAGTTACT 
ATCCACAATT TGGAAGAGTA CCTGAGATTG GTGATATTCT GGGCACTAAA TGAAGGTGTT 
TGTCGACAGT TTGACTCATT CAGAGATGGA TTTGAATCGG TCTTCCCGCT CTGCCATCTT 
CAGTACTTCT ACCCAGAAGA GCTGGACCAG CTCCTGTGTG GCAGTAAAGC AGACACTTGG 
GATGCAAAGA CATTGATGGA ATGCTGCAGG CCTGATCATG GCTATACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTGTTT 
CTCCAGTTTG TGACTGGTAG CCCACGATTG CCAGTTGGAG GATTTCGGAG TTTAAATCCA 
CCTCTGACAA TTGTGCGGAA GACATTTGAA TCAACAGAAA ACCCAGATGA CTTCTTACCA 
TCAGTAATGA CTTGTGTGAA CTATCTTAAA TTGCCGGACT ACTCGAGCAT TGACATAATG 
CGGGACAAAC TCTTGATAGC AGCAAGAGAA GGCCAACAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249325.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105185.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X46, mRNA.

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CloneID	OMu85022
Clone ID Related Accession (Same CDS sequence)	XM_030249325.1
Accession Version	XM_030249325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AAGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA TCCCTTAGCC AATTCTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTAATG AAAGAAGATC CGGAACTGGC TAAGTCTTTC 
ATTAAGACCT TGTTTGGTGT TCTCTATGAA GTGTACAGCT CCTCAGCAGG CCCTGCGGTC 
AGACACAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCTGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCAATGCT ATCCAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCTCTTCAG ATGGCAGAAA TCCTAATGCA AAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGGAGAGAA GGTGTGATGC ATCAAGTCAA ACATTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCCCCC AAGGCATGTA CAAATGGGTC AGGATCCCTG 
GGATCTACAA CACCAGCCAG CAGTGGGACT GCAACGGCAG CAACCAACGC CTCTGCTGAT 
CTGGGGTCAC CTAGCTTGCA GCATAGCAGA GATGATTCTT TGGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTCAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA 
TATTCCCCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCCTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA TATCGAACCA GCACGAACTG CAGGAGTTAG TGGTCTTGCC 
AGGGCAGCCT CAAAAGACAC CATATCCAAT AATAGAGAAA AAATTAAAGG CTGGATTAAG 
GAGCAGGCAC ATAAATTTGT TGAGCGTTAT TTTAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCCTTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCT 
TCATTTGAAA TCCAACATAG TGGGTTTGTG AAACAACTGT TGCTGTATTT GACATCTAAG 
AATGAAAAGG ATGCTGTCGG CAGAGAGATC CGGTTAAAAC GTTTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT GGAAGAGTAG AACCAGTGGG CCATGCACCT 
TTGCTGGCAT TGGTTCACAA GATGAACAAC TGTCTCAGCC AAATGGAGCA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGGAACGGA GCTGGAGGAA GAGGATCACA AGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGTCAG TTACAAAGAC ACCCAGACTG TGCAAATGTG 
AAGCAGTGGA AAGGCGGGCC TGTCAAGATT GATCCCCTGG CTCTGGTGCA AGCTATTGAG 
AGATACCTTG TGGTTAGAGG ATATGGAAGA GTAAGAGAAG ATGATGAAGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAGTCT TTGGCTGCTC AATTCTTAAA TTCGGGAAAT 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTAC TACCATATAA CATGACCGTG 
TATCAGGCAG TGCGGCAATT CAGTGTACAG GCTGAAGATG AAAGAGAATC TACTGATGAT 
GAGAGCAATC CTCTGGGAAG AGCTGGAATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGGG AGGATGAAGA AAGTACTAAA GATTGTGTTG GTGGCAAAAG GGGGAGAGCC 
CAAACAGCTC CAACAAAAAC TTCCCCCAGA AATGCAAAGA AGCATGATGA GTTATGGCAT 
GATGGAGTGT GCCCGTCAGT AGCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATCA CCTTTGAAGA CCCATCCTTA GATGTAATAC TTCTTTTAAG AGTTTTACAT 
GCCATCAGTC GATATTGGTA TTACTTGTAT GATAATGCGA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAATTAACA GCAAAAGCAA ATAGGCAGCT TCAAGACCCT 
CTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTCACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGACAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGGGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAC 
AGCAGAGTTG CGCCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTACTG 
AAACAAGCTG AGTCTGTGAT GCAGGACCTT GGCAGCTCTC GGGCCATGTT AGAAATCCAA 
TATGAAAATG AGGTTGGTAC AGGTCTGGGG CCAACACTGG AGTTTTATGC ACTTGTTTCT 
CAGGAACTAC AGAGAGCTGA CTTGTGTCTC TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAC ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCTAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCTCTTGGC 
CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG GAAACTTCAT TGACATCTCA TGATTTATTT 
GACATTGATC CTGTTGTAGC CAGATCAGTA TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTGG AGCAGGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTGGAAACT 
CTTACGATGA ATGGCTGTTC AGTGGAAGAT CTGGGATTGG ATTTTACGCT GCCTGGGTTT 
CCCAACATTG AATTGAAGAA AGGAGGGAAG GATATTCCAG TTACTATCCA CAATTTGGAA 
GAGTACCTGA GATTGGTGAT ATTCTGGGCA CTAAATGAAG GTGTTTGTCG ACAGTTTGAC 
TCATTCAGAG ATGGATTTGA ATCGGTCTTC CCGCTCTGCC ATCTTCAGTA CTTCTACCCA 
GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACATTG 
ATGGAATGCT GCAGGCCTGA TCATGGCTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACT 
GGTAGCCCAC GATTGCCAGT TGGAGGATTT CGGAGTTTAA ATCCACCTCT GACAATTGTG 
CGGAAGACAT TTGAATCAAC AGAAAACCCA GATGACTTCT TACCATCAGT AATGACTTGT 
GTGAACTATC TTAAATTGCC GGACTACTCG AGCATTGACA TAATGCGGGA CAAACTCTTG 
ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105185.1
CDS	363..6344
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249325.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X46, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249325.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AAGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA TCCCTTAGCC AATTCTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTAATG AAAGAAGATC CGGAACTGGC TAAGTCTTTC 
ATTAAGACCT TGTTTGGTGT TCTCTATGAA GTGTACAGCT CCTCAGCAGG CCCTGCGGTC 
AGACACAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCTGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCAATGCT ATCCAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCTCTTCAG ATGGCAGAAA TCCTAATGCA AAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGGAGAGAA GGTGTGATGC ATCAAGTCAA ACATTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCCCCC AAGGCATGTA CAAATGGGTC AGGATCCCTG 
GGATCTACAA CACCAGCCAG CAGTGGGACT GCAACGGCAG CAACCAACGC CTCTGCTGAT 
CTGGGGTCAC CTAGCTTGCA GCATAGCAGA GATGATTCTT TGGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTCAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA 
TATTCCCCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCCTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA TATCGAACCA GCACGAACTG CAGGAGTTAG TGGTCTTGCC 
AGGGCAGCCT CAAAAGACAC CATATCCAAT AATAGAGAAA AAATTAAAGG CTGGATTAAG 
GAGCAGGCAC ATAAATTTGT TGAGCGTTAT TTTAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCCTTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCT 
TCATTTGAAA TCCAACATAG TGGGTTTGTG AAACAACTGT TGCTGTATTT GACATCTAAG 
AATGAAAAGG ATGCTGTCGG CAGAGAGATC CGGTTAAAAC GTTTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT GGAAGAGTAG AACCAGTGGG CCATGCACCT 
TTGCTGGCAT TGGTTCACAA GATGAACAAC TGTCTCAGCC AAATGGAGCA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGGAACGGA GCTGGAGGAA GAGGATCACA AGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGTCAG TTACAAAGAC ACCCAGACTG TGCAAATGTG 
AAGCAGTGGA AAGGCGGGCC TGTCAAGATT GATCCCCTGG CTCTGGTGCA AGCTATTGAG 
AGATACCTTG TGGTTAGAGG ATATGGAAGA GTAAGAGAAG ATGATGAAGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAGTCT TTGGCTGCTC AATTCTTAAA TTCGGGAAAT 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTAC TACCATATAA CATGACCGTG 
TATCAGGCAG TGCGGCAATT CAGTGTACAG GCTGAAGATG AAAGAGAATC TACTGATGAT 
GAGAGCAATC CTCTGGGAAG AGCTGGAATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGGG AGGATGAAGA AAGTACTAAA GATTGTGTTG GTGGCAAAAG GGGGAGAGCC 
CAAACAGCTC CAACAAAAAC TTCCCCCAGA AATGCAAAGA AGCATGATGA GTTATGGCAT 
GATGGAGTGT GCCCGTCAGT AGCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATCA CCTTTGAAGA CCCATCCTTA GATGTAATAC TTCTTTTAAG AGTTTTACAT 
GCCATCAGTC GATATTGGTA TTACTTGTAT GATAATGCGA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAATTAACA GCAAAAGCAA ATAGGCAGCT TCAAGACCCT 
CTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTCACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGACAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGGGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAC 
AGCAGAGTTG CGCCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTACTG 
AAACAAGCTG AGTCTGTGAT GCAGGACCTT GGCAGCTCTC GGGCCATGTT AGAAATCCAA 
TATGAAAATG AGGTTGGTAC AGGTCTGGGG CCAACACTGG AGTTTTATGC ACTTGTTTCT 
CAGGAACTAC AGAGAGCTGA CTTGTGTCTC TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAC ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCTAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCTCTTGGC 
CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG GAAACTTCAT TGACATCTCA TGATTTATTT 
GACATTGATC CTGTTGTAGC CAGATCAGTA TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTGG AGCAGGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTGGAAACT 
CTTACGATGA ATGGCTGTTC AGTGGAAGAT CTGGGATTGG ATTTTACGCT GCCTGGGTTT 
CCCAACATTG AATTGAAGAA AGGAGGGAAG GATATTCCAG TTACTATCCA CAATTTGGAA 
GAGTACCTGA GATTGGTGAT ATTCTGGGCA CTAAATGAAG GTGTTTGTCG ACAGTTTGAC 
TCATTCAGAG ATGGATTTGA ATCGGTCTTC CCGCTCTGCC ATCTTCAGTA CTTCTACCCA 
GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACATTG 
ATGGAATGCT GCAGGCCTGA TCATGGCTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACT 
GGTAGCCCAC GATTGCCAGT TGGAGGATTT CGGAGTTTAA ATCCACCTCT GACAATTGTG 
CGGAAGACAT TTGAATCAAC AGAAAACCCA GATGACTTCT TACCATCAGT AATGACTTGT 
GTGAACTATC TTAAATTGCC GGACTACTCG AGCATTGACA TAATGCGGGA CAAACTCTTG 
ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496429.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496492.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X30, mRNA.

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CloneID	OMu27722
Clone ID Related Accession (Same CDS sequence)	XM_006496429.2 , XM_006496429.3
Accession Version	XM_006496429.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AAGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA TCCCTTAGCC AATTCTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTAATG AAAGAAGATC CGGAACTGGC TAAGTCTTTC 
ATTAAGACCT TGTTTGGTGT TCTCTATGAA GTGTACAGCT CCTCAGCAGG CCCTGCGGTC 
AGACACAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCTGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCAATGCT ATCCAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCTCTTCAG ATGGCAGAAA TCCTAATGCA AAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGGAGAGAA GGTGTGATGC ATCAAGTCAA ACATTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCCCCC AAGGCATGTA CAAATGGGTC AGGATCCCTG 
GGATCTACAA CACCAGCCAG CAGTGGGACT GCAACGGCAG CAACCAACGC CTCTGCTGAT 
CTGGGGTCAC CTAGCTTGCA GCATAGCAGA GATGATTCTT TGGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTCAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA 
TATTCCCCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCCTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA TATCGAACCA GCACGAACTG CAGGAGTTAG TGGTCTTGCC 
AGGGCAGCCT CAAAAGACAC CATATCCAAT AATAGAGAAA AAATTAAAGG CTGGATTAAG 
GAGCAGGCAC ATAAATTTGT TGAGCGTTAT TTTAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCCTTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCT 
TCATTTGAAA TCCAACATAG TGGGTTTGTG AAACAACTGT TGCTGTATTT GACATCTAAG 
AATGAAAAGG ATGCTGTCGG CAGAGAGATC CGGTTAAAAC GTTTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT GGAAGAGTAG AACCAGTGGG CCATGCACCT 
TTGCTGGCAT TGGTTCACAA GATGAACAAC TGTCTCAGCC AAATGGAGCA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGGAACGGA GCTGGAGGAA GCTTTTCTCT CAACAGAGGA 
TCACAAGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GTCAGTTACA AAGACACCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAAGGC GGGCCTGTCA AGATTGATCC CCTGGCTCTG 
GTGCAAGCTA TTGAGAGATA CCTTGTGGTT AGAGGATATG GAAGAGTAAG AGAAGATGAT 
GAAGATAGTG ATGATGATGG GTCAGATGAA GAAATAGATG AGTCTTTGGC TGCTCAATTC 
TTAAATTCGG GAAATGTAAG GCACAGGCTA CAGTTTTATA TTGGAGAACA TTTACTACCA 
TATAACATGA CCGTGTATCA GGCAGTGCGG CAATTCAGTG TACAGGCTGA AGATGAAAGA 
GAATCTACTG ATGATGAGAG CAATCCTCTG GGAAGAGCTG GAATTTGGAC AAAGACTCAT 
ACAATATGGT ACAAACCTGT GAGGGAGGAT GAAGAAAGTA CTAAAGATTG TGTTGGTGGC 
AAAAGGGGGA GAGCCCAAAC AGCTCCAACA AAAACTTCCC CCAGAAATGC AAAGAAGCAT 
GATGAGTTAT GGCATGATGG AGTGTGCCCG TCAGTAGCAA ATCCTTTAGA AGTTTACCTC 
ATTCCCACAC CACCTGAAAA TATCACCTTT GAAGACCCAT CCTTAGATGT AATACTTCTT 
TTAAGAGTTT TACATGCCAT CAGTCGATAT TGGTATTACT TGTATGATAA TGCGATGTGC 
AAGGAGATTA TTCCAACTAG TGAATTTATT AACAGTAAAT TAACAGCAAA AGCAAATAGG 
CAGCTTCAAG ACCCTCTAGT AATCATGACA GGAAACATCC CAACATGGCT CACTGAGCTA 
GGAAAAACCT GCCCATTTTT CTTTCCTTTT GATACCCGAC AAATGCTTTT TTATGTAACT 
GCATTTGATC GGGACCGGGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCTGATTCTC AAGACAGCAG AGTTGCGCCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TACTGAAACA AGCTGAGTCT GTGATGCAGG ACCTTGGCAG CTCTCGGGCC 
ATGTTAGAAA TCCAATATGA AAATGAGGTT GGTACAGGTC TGGGGCCAAC ACTGGAGTTT 
TATGCACTTG TTTCTCAGGA ACTACAGAGA GCTGACTTGT GTCTCTGGAG GGGTGAAGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CGCTTCCCTT TGGTAGGACA GCTAAGCCAG CTCACATCGC AAAGGTTAAG 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCTAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCTC TTGGCCTGCC CTTTTATAAG TGGATGCTGC GGCAGGAAAC TTCATTGACA 
TCTCATGATT TATTTGACAT TGATCCTGTT GTAGCCAGAT CAGTATATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTGGAGCAG GATAAATCCC AGACCAAAGA GAGTCTACAA 
TATGCATTGG AAACTCTTAC GATGAATGGC TGTTCAGTGG AAGATCTGGG ATTGGATTTT 
ACGCTGCCTG GGTTTCCCAA CATTGAATTG AAGAAAGGAG GGAAGGATAT TCCAGTTACT 
ATCCACAATT TGGAAGAGTA CCTGAGATTG GTGATATTCT GGGCACTAAA TGAAGGTGTT 
TGTCGACAGT TTGACTCATT CAGAGATGGA TTTGAATCGG TCTTCCCGCT CTGCCATCTT 
CAGTACTTCT ACCCAGAAGA GCTGGACCAG CTCCTGTGTG GCAGTAAAGC AGACACTTGG 
GATGCAAAGA CATTGATGGA ATGCTGCAGG CCTGATCATG GCTATACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTGTTT 
CTCCAGTTTG TGACTGGTAG CCCACGATTG CCAGTTGGAG GATTTCGGAG TTTAAATCCA 
CCTCTGACAA TTGTGCGGAA GACATTTGAA TCAACAGAAA ACCCAGATGA CTTCTTACCA 
TCAGTAATGA CTTGTGTGAA CTATCTTAAA TTGCCGGACT ACTCGAGCAT TGACATAATG 
CGGGACAAAC TCTTGATAGC AGCAAGAGAA GGCCAACAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496492.1
CDS	400..6396
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496429.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496429.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AAGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA TCCCTTAGCC AATTCTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTAATG AAAGAAGATC CGGAACTGGC TAAGTCTTTC 
ATTAAGACCT TGTTTGGTGT TCTCTATGAA GTGTACAGCT CCTCAGCAGG CCCTGCGGTC 
AGACACAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCTGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCAATGCT ATCCAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCTCTTCAG ATGGCAGAAA TCCTAATGCA AAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGGAGAGAA GGTGTGATGC ATCAAGTCAA ACATTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCCCCC AAGGCATGTA CAAATGGGTC AGGATCCCTG 
GGATCTACAA CACCAGCCAG CAGTGGGACT GCAACGGCAG CAACCAACGC CTCTGCTGAT 
CTGGGGTCAC CTAGCTTGCA GCATAGCAGA GATGATTCTT TGGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTCAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA 
TATTCCCCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCCTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA TATCGAACCA GCACGAACTG CAGGAGTTAG TGGTCTTGCC 
AGGGCAGCCT CAAAAGACAC CATATCCAAT AATAGAGAAA AAATTAAAGG CTGGATTAAG 
GAGCAGGCAC ATAAATTTGT TGAGCGTTAT TTTAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCCTTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCT 
TCATTTGAAA TCCAACATAG TGGGTTTGTG AAACAACTGT TGCTGTATTT GACATCTAAG 
AATGAAAAGG ATGCTGTCGG CAGAGAGATC CGGTTAAAAC GTTTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT GGAAGAGTAG AACCAGTGGG CCATGCACCT 
TTGCTGGCAT TGGTTCACAA GATGAACAAC TGTCTCAGCC AAATGGAGCA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGGAACGGA GCTGGAGGAA GCTTTTCTCT CAACAGAGGA 
TCACAAGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GTCAGTTACA AAGACACCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAAGGC GGGCCTGTCA AGATTGATCC CCTGGCTCTG 
GTGCAAGCTA TTGAGAGATA CCTTGTGGTT AGAGGATATG GAAGAGTAAG AGAAGATGAT 
GAAGATAGTG ATGATGATGG GTCAGATGAA GAAATAGATG AGTCTTTGGC TGCTCAATTC 
TTAAATTCGG GAAATGTAAG GCACAGGCTA CAGTTTTATA TTGGAGAACA TTTACTACCA 
TATAACATGA CCGTGTATCA GGCAGTGCGG CAATTCAGTG TACAGGCTGA AGATGAAAGA 
GAATCTACTG ATGATGAGAG CAATCCTCTG GGAAGAGCTG GAATTTGGAC AAAGACTCAT 
ACAATATGGT ACAAACCTGT GAGGGAGGAT GAAGAAAGTA CTAAAGATTG TGTTGGTGGC 
AAAAGGGGGA GAGCCCAAAC AGCTCCAACA AAAACTTCCC CCAGAAATGC AAAGAAGCAT 
GATGAGTTAT GGCATGATGG AGTGTGCCCG TCAGTAGCAA ATCCTTTAGA AGTTTACCTC 
ATTCCCACAC CACCTGAAAA TATCACCTTT GAAGACCCAT CCTTAGATGT AATACTTCTT 
TTAAGAGTTT TACATGCCAT CAGTCGATAT TGGTATTACT TGTATGATAA TGCGATGTGC 
AAGGAGATTA TTCCAACTAG TGAATTTATT AACAGTAAAT TAACAGCAAA AGCAAATAGG 
CAGCTTCAAG ACCCTCTAGT AATCATGACA GGAAACATCC CAACATGGCT CACTGAGCTA 
GGAAAAACCT GCCCATTTTT CTTTCCTTTT GATACCCGAC AAATGCTTTT TTATGTAACT 
GCATTTGATC GGGACCGGGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCTGATTCTC AAGACAGCAG AGTTGCGCCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TACTGAAACA AGCTGAGTCT GTGATGCAGG ACCTTGGCAG CTCTCGGGCC 
ATGTTAGAAA TCCAATATGA AAATGAGGTT GGTACAGGTC TGGGGCCAAC ACTGGAGTTT 
TATGCACTTG TTTCTCAGGA ACTACAGAGA GCTGACTTGT GTCTCTGGAG GGGTGAAGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CGCTTCCCTT TGGTAGGACA GCTAAGCCAG CTCACATCGC AAAGGTTAAG 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCTAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCTC TTGGCCTGCC CTTTTATAAG TGGATGCTGC GGCAGGAAAC TTCATTGACA 
TCTCATGATT TATTTGACAT TGATCCTGTT GTAGCCAGAT CAGTATATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTGGAGCAG GATAAATCCC AGACCAAAGA GAGTCTACAA 
TATGCATTGG AAACTCTTAC GATGAATGGC TGTTCAGTGG AAGATCTGGG ATTGGATTTT 
ACGCTGCCTG GGTTTCCCAA CATTGAATTG AAGAAAGGAG GGAAGGATAT TCCAGTTACT 
ATCCACAATT TGGAAGAGTA CCTGAGATTG GTGATATTCT GGGCACTAAA TGAAGGTGTT 
TGTCGACAGT TTGACTCATT CAGAGATGGA TTTGAATCGG TCTTCCCGCT CTGCCATCTT 
CAGTACTTCT ACCCAGAAGA GCTGGACCAG CTCCTGTGTG GCAGTAAAGC AGACACTTGG 
GATGCAAAGA CATTGATGGA ATGCTGCAGG CCTGATCATG GCTATACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTGTTT 
CTCCAGTTTG TGACTGGTAG CCCACGATTG CCAGTTGGAG GATTTCGGAG TTTAAATCCA 
CCTCTGACAA TTGTGCGGAA GACATTTGAA TCAACAGAAA ACCCAGATGA CTTCTTACCA 
TCAGTAATGA CTTGTGTGAA CTATCTTAAA TTGCCGGACT ACTCGAGCAT TGACATAATG 
CGGGACAAAC TCTTGATAGC AGCAAGAGAA GGCCAACAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238674.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236976.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X47, mRNA.

Price & Availability↑

CloneID	OMu67894
Clone ID Related Accession (Same CDS sequence)	XM_011238674.2
Accession Version	XM_011238674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCAGT TATTGGTCAT GGGGAATGAG GAGACACTAG GAGGGTTTCC TGTGAAGAGT 
GTTGTTCCAG CTTTGATAAC ATTACTGCAG ATGGAACATA ATTTTGACAT TATGAATCAT 
GCTTGCCGAG CCTTAACATA TATGATGGAA GCTCTCCCCC GGTCATCTGC TGTTGTAGTG 
GATGCTATCC CTGTTTTCTT AGAAAAGTTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGCGACACA GTAAAGCCAT TTTACAAGCG 
GGTGGTTTGG CAGATTGCTT GTTGTATCTA GAATTCTTCA GCATCAATGC CCAAAGAAAT 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACAC CAGATGAGTT TCATTTTGTG 
GCAGATTCCC TACCATTGCT TACCCAAAGG CTAACTCATC AGGACAAAAA GTCAGTAGAA 
AGTACTTGCC TTTGTTTTGC ACGTTTAGTG GACAACTTTC AACATGAGGA GAACTTACTG 
CAACAGGTTG CTTCCAAAGA TCTACTTACA AATGTTCAAC AATTGTTGGT AGTGACTCCA 
CCGATTTTAA GTTCTGGGAT GTTTATTATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CATTAGCTGT TCAACTTATG AAACAAAGCT TTACAGACAT TGCAGAAACA 
CTTCACTTCC TGCTATGTGG TGCCTCCAAT GGAAGTTGTC AAGAGCAGAT TGACCTTGTT 
CCACGAAGTC CTCAAGAGCT GTATGAGCTG ACATCTTTGA TTTGTGAACT TATGCCATGT 
TTACCAAAAG AAGGCATCTT CGCAGTTGAT ACCATGTTGA AAAAGGGAAA TGCACAGAAC 
ACAGATGGTG CAATATGGCA GTGGCGGGAT GACCGAGGCC TCTGGCATCC CTATAACAGG 
ATTGACAGCC GGATCATTGA GGTAGCAGCC CATCAGGTCG GTGAGGATGA GATAAGCTTG 
TCCACTCTGG GACGAGTTTA TACTATTGAT TTTAATTCTA TGCAGCAAAT CAATGAAGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAATCCCT TAGCCAATTC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGATCCGGAA 
CTGGCTAAGT CTTTCATTAA GACCTTGTTT GGTGTTCTCT ATGAAGTGTA CAGCTCCTCA 
GCAGGCCCTG CGGTCAGACA CAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTTGCT 
GATGCTGAAC TTCTGAAGGA TGTTCTGAAA AATCATGCTG TTTCAAGTCA CATTGCTTCA 
ATGCTATCCA GCCAAGACCT GAAGATAGTA GTTGGAGCTC TTCAGATGGC AGAAATCCTA 
ATGCAAAAGT TACCTGATAT TTTTAGTGTT TACTTCAGGA GAGAAGGTGT GATGCATCAA 
GTCAAACATT TAGCAGAATC AGAGTCTTTG TTGACAAGTC CCCCCAAGGC ATGTACAAAT 
GGGTCAGGAT CCCTGGGATC TACAACACCA GCCAGCAGTG GGACTGCAAC GGCAGCAACC 
AACGCCTCTG CTGATCTGGG GTCACCTAGC TTGCAGCATA GCAGAGATGA TTCTTTGGAT 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTCAAGAGAA AACGACTGCC AAAACGAGGG 
CCAAGAAGGC CAAAATATTC CCCTCCAAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAATCT TCCTTCCTGG CAAGCTTGAA TCCAAAAACA 
TGGGGAAGGT TAAGCGCACA GTCCAACAGC AACAATATCG AACCAGCACG AACTGCAGGA 
GTTAGTGGTC TTGCCAGGGC AGCCTCAAAA GACACCATAT CCAATAATAG AGAAAAAATT 
AAAGGCTGGA TTAAGGAGCA GGCACATAAA TTTGTTGAGC GTTATTTTAG TTCTGAGAAT 
ATGGATGGAA GCAACCCTGC CTTGAATGTC CTTCAGAGAC TTTGTGCTGC AACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCAGATG TTTCTTCATT TGAAATCCAA CATAGTGGGT TTGTGAAACA ACTGTTGCTG 
TATTTGACAT CTAAGAATGA AAAGGATGCT GTCGGCAGAG AGATCCGGTT AAAACGTTTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CTGTTGGAAG AGTAGAACCA 
GTGGGCCATG CACCTTTGCT GGCATTGGTT CACAAGATGA ACAACTGTCT CAGCCAAATG 
GAGCAATTTC CAGTCAAAGT ACATGATTTC CCTAGTGGGA ACGGAGCTGG AGGAAGCTTT 
TCTCTCAACA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG TATTTTCCGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236976.1
CDS	176..4876
Translation	MCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238674.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X47, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238674.2

ORF Insert Sequence:

ATGTGTCAGT TATTGGTCAT GGGGAATGAG GAGACACTAG GAGGGTTTCC TGTGAAGAGT 
GTTGTTCCAG CTTTGATAAC ATTACTGCAG ATGGAACATA ATTTTGACAT TATGAATCAT 
GCTTGCCGAG CCTTAACATA TATGATGGAA GCTCTCCCCC GGTCATCTGC TGTTGTAGTG 
GATGCTATCC CTGTTTTCTT AGAAAAGTTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGCGACACA GTAAAGCCAT TTTACAAGCG 
GGTGGTTTGG CAGATTGCTT GTTGTATCTA GAATTCTTCA GCATCAATGC CCAAAGAAAT 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACAC CAGATGAGTT TCATTTTGTG 
GCAGATTCCC TACCATTGCT TACCCAAAGG CTAACTCATC AGGACAAAAA GTCAGTAGAA 
AGTACTTGCC TTTGTTTTGC ACGTTTAGTG GACAACTTTC AACATGAGGA GAACTTACTG 
CAACAGGTTG CTTCCAAAGA TCTACTTACA AATGTTCAAC AATTGTTGGT AGTGACTCCA 
CCGATTTTAA GTTCTGGGAT GTTTATTATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CATTAGCTGT TCAACTTATG AAACAAAGCT TTACAGACAT TGCAGAAACA 
CTTCACTTCC TGCTATGTGG TGCCTCCAAT GGAAGTTGTC AAGAGCAGAT TGACCTTGTT 
CCACGAAGTC CTCAAGAGCT GTATGAGCTG ACATCTTTGA TTTGTGAACT TATGCCATGT 
TTACCAAAAG AAGGCATCTT CGCAGTTGAT ACCATGTTGA AAAAGGGAAA TGCACAGAAC 
ACAGATGGTG CAATATGGCA GTGGCGGGAT GACCGAGGCC TCTGGCATCC CTATAACAGG 
ATTGACAGCC GGATCATTGA GGTAGCAGCC CATCAGGTCG GTGAGGATGA GATAAGCTTG 
TCCACTCTGG GACGAGTTTA TACTATTGAT TTTAATTCTA TGCAGCAAAT CAATGAAGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAATCCCT TAGCCAATTC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGATCCGGAA 
CTGGCTAAGT CTTTCATTAA GACCTTGTTT GGTGTTCTCT ATGAAGTGTA CAGCTCCTCA 
GCAGGCCCTG CGGTCAGACA CAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTTGCT 
GATGCTGAAC TTCTGAAGGA TGTTCTGAAA AATCATGCTG TTTCAAGTCA CATTGCTTCA 
ATGCTATCCA GCCAAGACCT GAAGATAGTA GTTGGAGCTC TTCAGATGGC AGAAATCCTA 
ATGCAAAAGT TACCTGATAT TTTTAGTGTT TACTTCAGGA GAGAAGGTGT GATGCATCAA 
GTCAAACATT TAGCAGAATC AGAGTCTTTG TTGACAAGTC CCCCCAAGGC ATGTACAAAT 
GGGTCAGGAT CCCTGGGATC TACAACACCA GCCAGCAGTG GGACTGCAAC GGCAGCAACC 
AACGCCTCTG CTGATCTGGG GTCACCTAGC TTGCAGCATA GCAGAGATGA TTCTTTGGAT 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTCAAGAGAA AACGACTGCC AAAACGAGGG 
CCAAGAAGGC CAAAATATTC CCCTCCAAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAATCT TCCTTCCTGG CAAGCTTGAA TCCAAAAACA 
TGGGGAAGGT TAAGCGCACA GTCCAACAGC AACAATATCG AACCAGCACG AACTGCAGGA 
GTTAGTGGTC TTGCCAGGGC AGCCTCAAAA GACACCATAT CCAATAATAG AGAAAAAATT 
AAAGGCTGGA TTAAGGAGCA GGCACATAAA TTTGTTGAGC GTTATTTTAG TTCTGAGAAT 
ATGGATGGAA GCAACCCTGC CTTGAATGTC CTTCAGAGAC TTTGTGCTGC AACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCAGATG TTTCTTCATT TGAAATCCAA CATAGTGGGT TTGTGAAACA ACTGTTGCTG 
TATTTGACAT CTAAGAATGA AAAGGATGCT GTCGGCAGAG AGATCCGGTT AAAACGTTTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CTGTTGGAAG AGTAGAACCA 
GTGGGCCATG CACCTTTGCT GGCATTGGTT CACAAGATGA ACAACTGTCT CAGCCAAATG 
GAGCAATTTC CAGTCAAAGT ACATGATTTC CCTAGTGGGA ACGGAGCTGG AGGAAGCTTT 
TCTCTCAACA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG TATTTTCCGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496409.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496472.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu27709
Clone ID Related Accession (Same CDS sequence)	XM_006496409.3 , XM_006496409.4
Accession Version	XM_006496409.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496409.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AAGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA TCCCTTAGCC 
AATTCTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTAATG 
AAAGAAGATC CGGAACTGGC TAAGTCTTTC ATTAAGACCT TGTTTGGTGT TCTCTATGAA 
GTGTACAGCT CCTCAGCAGG CCCTGCGGTC AGACACAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCTGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCAATGCT ATCCAGCCAA GACCTGAAGA TAGTAGTTGG AGCTCTTCAG 
ATGGCAGAAA TCCTAATGCA AAAGTTACCT GATATTTTTA GTGTTTACTT CAGGAGAGAA 
GGTGTGATGC ATCAAGTCAA ACATTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCCCCC 
AAGGCATGTA CAAATGGGTC AGGATCCCTG GGATCTACAA CACCAGCCAG CAGTGGGACT 
GCAACGGCAG CAACCAACGC CTCTGCTGAT CTGGGGTCAC CTAGCTTGCA GCATAGCAGA 
GATGATTCTT TGGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTCAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA TATTCCCCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCCTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA TATCGAACCA 
GCACGAACTG CAGGAGTTAG TGGTCTTGCC AGGGCAGCCT CAAAAGACAC CATATCCAAT 
AATAGAGAAA AAATTAAAGG CTGGATTAAG GAGCAGGCAC ATAAATTTGT TGAGCGTTAT 
TTTAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCCTTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCT TCATTTGAAA TCCAACATAG TGGGTTTGTG 
AAACAACTGT TGCTGTATTT GACATCTAAG AATGAAAAGG ATGCTGTCGG CAGAGAGATC 
CGGTTAAAAC GTTTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT 
GGAAGAGTAG AACCAGTGGG CCATGCACCT TTGCTGGCAT TGGTTCACAA GATGAACAAC 
TGTCTCAGCC AAATGGAGCA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGGAACGGA 
GCTGGAGGAA GCTTTTCTCT CAACAGAGGA TCACAAGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GTCAGTTACA AAGACACCCA GACTGTGCAA ATGTGAAGCA GTGGAAAGGC 
GGGCCTGTCA AGATTGATCC CCTGGCTCTG GTGCAAGCTA TTGAGAGATA CCTTGTGGTT 
AGAGGTATTT TCCGATATGG AAGAGTAAGA GAAGATGATG AAGATAGTGA TGATGATGGG 
TCAGATGAAG AAATAGATGA GTCTTTGGCT GCTCAATTCT TAAATTCGGG AAATGTAAGG 
CACAGGCTAC AGTTTTATAT TGGAGAACAT TTACTACCAT ATAACATGAC CGTGTATCAG 
GCAGTGCGGC AATTCAGTGT ACAGGCTGAA GATGAAAGAG AATCTACTGA TGATGAGAGC 
AATCCTCTGG GAAGAGCTGG AATTTGGACA AAGACTCATA CAATATGGTA CAAACCTGTG 
AGGGAGGATG AAGAAAGTAC TAAAGATTGT GTTGGTGGCA AAAGGGGGAG AGCCCAAACA 
GCTCCAACAA AAACTTCCCC CAGAAATGCA AAGAAGCATG ATGAGTTATG GCATGATGGA 
GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATCACCTTTG AAGACCCATC CTTAGATGTA ATACTTCTTT TAAGAGTTTT ACATGCCATC 
AGTCGATATT GGTATTACTT GTATGATAAT GCGATGTGCA AGGAGATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAATT AACAGCAAAA GCAAATAGGC AGCTTCAAGA CCCTCTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTC ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGACA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGGGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGACAGCAGA 
GTTGCGCCTA GATTGGATAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT ACTGAAACAA 
GCTGAGTCTG TGATGCAGGA CCTTGGCAGC TCTCGGGCCA TGTTAGAAAT CCAATATGAA 
AATGAGGTTG GTACAGGTCT GGGGCCAACA CTGGAGTTTT ATGCACTTGT TTCTCAGGAA 
CTACAGAGAG CTGACTTGTG TCTCTGGAGG GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCACATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CTAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCTCT TGGCCTGCCC 
TTTTATAAGT GGATGCTGCG GCAGGAAACT TCATTGACAT CTCATGATTT ATTTGACATT 
GATCCTGTTG TAGCCAGATC AGTATATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTGGAGCAGG ATAAATCCCA GACCAAAGAG AGTCTACAAT ATGCATTGGA AACTCTTACG 
ATGAATGGCT GTTCAGTGGA AGATCTGGGA TTGGATTTTA CGCTGCCTGG GTTTCCCAAC 
ATTGAATTGA AGAAAGGAGG GAAGGATATT CCAGTTACTA TCCACAATTT GGAAGAGTAC 
CTGAGATTGG TGATATTCTG GGCACTAAAT GAAGGTGTTT GTCGACAGTT TGACTCATTC 
AGAGATGGAT TTGAATCGGT CTTCCCGCTC TGCCATCTTC AGTACTTCTA CCCAGAAGAG 
CTGGACCAGC TCCTGTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ATTGATGGAA 
TGCTGCAGGC CTGATCATGG CTATACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTGTTTC TCCAGTTTGT GACTGGTAGC 
CCACGATTGC CAGTTGGAGG ATTTCGGAGT TTAAATCCAC CTCTGACAAT TGTGCGGAAG 
ACATTTGAAT CAACAGAAAA CCCAGATGAC TTCTTACCAT CAGTAATGAC TTGTGTGAAC 
TATCTTAAAT TGCCGGACTA CTCGAGCATT GACATAATGC GGGACAAACT CTTGATAGCA 
GCAAGAGAAG GCCAACAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496472.1
CDS	363..6578
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496409.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496409.4

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AAGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA TCCCTTAGCC 
AATTCTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTAATG 
AAAGAAGATC CGGAACTGGC TAAGTCTTTC ATTAAGACCT TGTTTGGTGT TCTCTATGAA 
GTGTACAGCT CCTCAGCAGG CCCTGCGGTC AGACACAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCTGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCAATGCT ATCCAGCCAA GACCTGAAGA TAGTAGTTGG AGCTCTTCAG 
ATGGCAGAAA TCCTAATGCA AAAGTTACCT GATATTTTTA GTGTTTACTT CAGGAGAGAA 
GGTGTGATGC ATCAAGTCAA ACATTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCCCCC 
AAGGCATGTA CAAATGGGTC AGGATCCCTG GGATCTACAA CACCAGCCAG CAGTGGGACT 
GCAACGGCAG CAACCAACGC CTCTGCTGAT CTGGGGTCAC CTAGCTTGCA GCATAGCAGA 
GATGATTCTT TGGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTCAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA TATTCCCCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCCTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA TATCGAACCA 
GCACGAACTG CAGGAGTTAG TGGTCTTGCC AGGGCAGCCT CAAAAGACAC CATATCCAAT 
AATAGAGAAA AAATTAAAGG CTGGATTAAG GAGCAGGCAC ATAAATTTGT TGAGCGTTAT 
TTTAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCCTTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCT TCATTTGAAA TCCAACATAG TGGGTTTGTG 
AAACAACTGT TGCTGTATTT GACATCTAAG AATGAAAAGG ATGCTGTCGG CAGAGAGATC 
CGGTTAAAAC GTTTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT 
GGAAGAGTAG AACCAGTGGG CCATGCACCT TTGCTGGCAT TGGTTCACAA GATGAACAAC 
TGTCTCAGCC AAATGGAGCA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGGAACGGA 
GCTGGAGGAA GCTTTTCTCT CAACAGAGGA TCACAAGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GTCAGTTACA AAGACACCCA GACTGTGCAA ATGTGAAGCA GTGGAAAGGC 
GGGCCTGTCA AGATTGATCC CCTGGCTCTG GTGCAAGCTA TTGAGAGATA CCTTGTGGTT 
AGAGGTATTT TCCGATATGG AAGAGTAAGA GAAGATGATG AAGATAGTGA TGATGATGGG 
TCAGATGAAG AAATAGATGA GTCTTTGGCT GCTCAATTCT TAAATTCGGG AAATGTAAGG 
CACAGGCTAC AGTTTTATAT TGGAGAACAT TTACTACCAT ATAACATGAC CGTGTATCAG 
GCAGTGCGGC AATTCAGTGT ACAGGCTGAA GATGAAAGAG AATCTACTGA TGATGAGAGC 
AATCCTCTGG GAAGAGCTGG AATTTGGACA AAGACTCATA CAATATGGTA CAAACCTGTG 
AGGGAGGATG AAGAAAGTAC TAAAGATTGT GTTGGTGGCA AAAGGGGGAG AGCCCAAACA 
GCTCCAACAA AAACTTCCCC CAGAAATGCA AAGAAGCATG ATGAGTTATG GCATGATGGA 
GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATCACCTTTG AAGACCCATC CTTAGATGTA ATACTTCTTT TAAGAGTTTT ACATGCCATC 
AGTCGATATT GGTATTACTT GTATGATAAT GCGATGTGCA AGGAGATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAATT AACAGCAAAA GCAAATAGGC AGCTTCAAGA CCCTCTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTC ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGACA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGGGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGACAGCAGA 
GTTGCGCCTA GATTGGATAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT ACTGAAACAA 
GCTGAGTCTG TGATGCAGGA CCTTGGCAGC TCTCGGGCCA TGTTAGAAAT CCAATATGAA 
AATGAGGTTG GTACAGGTCT GGGGCCAACA CTGGAGTTTT ATGCACTTGT TTCTCAGGAA 
CTACAGAGAG CTGACTTGTG TCTCTGGAGG GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCACATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CTAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCTCT TGGCCTGCCC 
TTTTATAAGT GGATGCTGCG GCAGGAAACT TCATTGACAT CTCATGATTT ATTTGACATT 
GATCCTGTTG TAGCCAGATC AGTATATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTGGAGCAGG ATAAATCCCA GACCAAAGAG AGTCTACAAT ATGCATTGGA AACTCTTACG 
ATGAATGGCT GTTCAGTGGA AGATCTGGGA TTGGATTTTA CGCTGCCTGG GTTTCCCAAC 
ATTGAATTGA AGAAAGGAGG GAAGGATATT CCAGTTACTA TCCACAATTT GGAAGAGTAC 
CTGAGATTGG TGATATTCTG GGCACTAAAT GAAGGTGTTT GTCGACAGTT TGACTCATTC 
AGAGATGGAT TTGAATCGGT CTTCCCGCTC TGCCATCTTC AGTACTTCTA CCCAGAAGAG 
CTGGACCAGC TCCTGTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ATTGATGGAA 
TGCTGCAGGC CTGATCATGG CTATACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTGTTTC TCCAGTTTGT GACTGGTAGC 
CCACGATTGC CAGTTGGAGG ATTTCGGAGT TTAAATCCAC CTCTGACAAT TGTGCGGAAG 
ACATTTGAAT CAACAGAAAA CCCAGATGAC TTCTTACCAT CAGTAATGAC TTGTGTGAAC 
TATCTTAAAT TGCCGGACTA CTCGAGCATT GACATAATGC GGGACAAACT CTTGATAGCA 
GCAAGAGAAG GCCAACAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496414.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496477.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X12, mRNA.

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CloneID	OMu27710
Clone ID Related Accession (Same CDS sequence)	XM_006496414.3 , XM_006496414.4
Accession Version	XM_006496414.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496414.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAAG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAATCC CTTAGCCAAT 
TCTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTAATGAAA 
GAAGATCCGG AACTGGCTAA GTCTTTCATT AAGACCTTGT TTGGTGTTCT CTATGAAGTG 
TACAGCTCCT CAGCAGGCCC TGCGGTCAGA CACAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CTGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CAATGCTATC CAGCCAAGAC CTGAAGATAG TAGTTGGAGC TCTTCAGATG 
GCAGAAATCC TAATGCAAAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG GAGAGAAGGT 
GTGATGCATC AAGTCAAACA TTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCCCCCAAG 
GCATGTACAA ATGGGTCAGG ATCCCTGGGA TCTACAACAC CAGCCAGCAG TGGGACTGCA 
ACGGCAGCAA CCAACGCCTC TGCTGATCTG GGGTCACCTA GCTTGCAGCA TAGCAGAGAT 
GATTCTTTGG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTCAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAATAT TCCCCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCCTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAATAT CGAACCAGCA 
CGAACTGCAG GAGTTAGTGG TCTTGCCAGG GCAGCCTCAA AAGACACCAT ATCCAATAAT 
AGAGAAAAAA TTAAAGGCTG GATTAAGGAG CAGGCACATA AATTTGTTGA GCGTTATTTT 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCCTTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCTTCA TTTGAAATCC AACATAGTGG GTTTGTGAAA 
CAACTGTTGC TGTATTTGAC ATCTAAGAAT GAAAAGGATG CTGTCGGCAG AGAGATCCGG 
TTAAAACGTT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCTGTTGGA 
AGAGTAGAAC CAGTGGGCCA TGCACCTTTG CTGGCATTGG TTCACAAGAT GAACAACTGT 
CTCAGCCAAA TGGAGCAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG GAACGGAGCT 
GGAGGAAGCT TTTCTCTCAA CAGAGGATCA CAAGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGTC AGTTACAAAG ACACCCAGAC TGTGCAAATG TGAAGCAGTG GAAAGGCGGG 
CCTGTCAAGA TTGATCCCCT GGCTCTGGTG CAAGCTATTG AGAGATACCT TGTGGTTAGA 
GGTATTTTCC GATATGGAAG AGTAAGAGAA GATGATGAAG ATAGTGATGA TGATGGGTCA 
GATGAAGAAA TAGATGAGTC TTTGGCTGCT CAATTCTTAA ATTCGGGAAA TGTAAGGCAC 
AGGCTACAGT TTTATATTGG AGAACATTTA CTACCATATA ACATGACCGT GTATCAGGCA 
GTGCGGCAAT TCAGTGTACA GGCTGAAGAT GAAAGAGAAT CTACTGATGA TGAGAGCAAT 
CCTCTGGGAA GAGCTGGAAT TTGGACAAAG ACTCATACAA TATGGTACAA ACCTGTGAGG 
GAGGATGAAG AAAGTACTAA AGATTGTGTT GGTGGCAAAA GGGGGAGAGC CCAAACAGCT 
CCAACAAAAA CTTCCCCCAG AAATGCAAAG AAGCATGATG AGTTATGGCA TGATGGAGTG 
TGCCCGTCAG TAGCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATC 
ACCTTTGAAG ACCCATCCTT AGATGTAATA CTTCTTTTAA GAGTTTTACA TGCCATCAGT 
CGATATTGGT ATTACTTGTA TGATAATGCG ATGTGCAAGG AGATTATTCC AACTAGTGAA 
TTTATTAACA GTAAATTAAC AGCAAAAGCA AATAGGCAGC TTCAAGACCC TCTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTCACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGACAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGGGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA CAGCAGAGTT 
GCGCCTAGAT TGGATAGAAA AAAACGTACT GTGAACAGAG AGGAGCTACT GAAACAAGCT 
GAGTCTGTGA TGCAGGACCT TGGCAGCTCT CGGGCCATGT TAGAAATCCA ATATGAAAAT 
GAGGTTGGTA CAGGTCTGGG GCCAACACTG GAGTTTTATG CACTTGTTTC TCAGGAACTA 
CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCTA AGCCAGCTCA CATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCTA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCTCTTGG CCTGCCCTTT 
TATAAGTGGA TGCTGCGGCA GGAAACTTCA TTGACATCTC ATGATTTATT TGACATTGAT 
CCTGTTGTAG CCAGATCAGT ATATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTG 
GAGCAGGATA AATCCCAGAC CAAAGAGAGT CTACAATATG CATTGGAAAC TCTTACGATG 
AATGGCTGTT CAGTGGAAGA TCTGGGATTG GATTTTACGC TGCCTGGGTT TCCCAACATT 
GAATTGAAGA AAGGAGGGAA GGATATTCCA GTTACTATCC ACAATTTGGA AGAGTACCTG 
AGATTGGTGA TATTCTGGGC ACTAAATGAA GGTGTTTGTC GACAGTTTGA CTCATTCAGA 
GATGGATTTG AATCGGTCTT CCCGCTCTGC CATCTTCAGT ACTTCTACCC AGAAGAGCTG 
GACCAGCTCC TGTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACATT GATGGAATGC 
TGCAGGCCTG ATCATGGCTA TACTCATGAC AGTCGGGCTG TGAAGTTCTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA 
CGATTGCCAG TTGGAGGATT TCGGAGTTTA AATCCACCTC TGACAATTGT GCGGAAGACA 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTACCATCAG TAATGACTTG TGTGAACTAT 
CTTAAATTGC CGGACTACTC GAGCATTGAC ATAATGCGGG ACAAACTCTT GATAGCAGCA 
AGAGAAGGCC AACAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496477.1
CDS	363..6575
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496414.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496414.4

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAAG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAATCC CTTAGCCAAT 
TCTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTAATGAAA 
GAAGATCCGG AACTGGCTAA GTCTTTCATT AAGACCTTGT TTGGTGTTCT CTATGAAGTG 
TACAGCTCCT CAGCAGGCCC TGCGGTCAGA CACAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CTGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CAATGCTATC CAGCCAAGAC CTGAAGATAG TAGTTGGAGC TCTTCAGATG 
GCAGAAATCC TAATGCAAAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG GAGAGAAGGT 
GTGATGCATC AAGTCAAACA TTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCCCCCAAG 
GCATGTACAA ATGGGTCAGG ATCCCTGGGA TCTACAACAC CAGCCAGCAG TGGGACTGCA 
ACGGCAGCAA CCAACGCCTC TGCTGATCTG GGGTCACCTA GCTTGCAGCA TAGCAGAGAT 
GATTCTTTGG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTCAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAATAT TCCCCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCCTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAATAT CGAACCAGCA 
CGAACTGCAG GAGTTAGTGG TCTTGCCAGG GCAGCCTCAA AAGACACCAT ATCCAATAAT 
AGAGAAAAAA TTAAAGGCTG GATTAAGGAG CAGGCACATA AATTTGTTGA GCGTTATTTT 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCCTTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCTTCA TTTGAAATCC AACATAGTGG GTTTGTGAAA 
CAACTGTTGC TGTATTTGAC ATCTAAGAAT GAAAAGGATG CTGTCGGCAG AGAGATCCGG 
TTAAAACGTT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCTGTTGGA 
AGAGTAGAAC CAGTGGGCCA TGCACCTTTG CTGGCATTGG TTCACAAGAT GAACAACTGT 
CTCAGCCAAA TGGAGCAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG GAACGGAGCT 
GGAGGAAGCT TTTCTCTCAA CAGAGGATCA CAAGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGTC AGTTACAAAG ACACCCAGAC TGTGCAAATG TGAAGCAGTG GAAAGGCGGG 
CCTGTCAAGA TTGATCCCCT GGCTCTGGTG CAAGCTATTG AGAGATACCT TGTGGTTAGA 
GGTATTTTCC GATATGGAAG AGTAAGAGAA GATGATGAAG ATAGTGATGA TGATGGGTCA 
GATGAAGAAA TAGATGAGTC TTTGGCTGCT CAATTCTTAA ATTCGGGAAA TGTAAGGCAC 
AGGCTACAGT TTTATATTGG AGAACATTTA CTACCATATA ACATGACCGT GTATCAGGCA 
GTGCGGCAAT TCAGTGTACA GGCTGAAGAT GAAAGAGAAT CTACTGATGA TGAGAGCAAT 
CCTCTGGGAA GAGCTGGAAT TTGGACAAAG ACTCATACAA TATGGTACAA ACCTGTGAGG 
GAGGATGAAG AAAGTACTAA AGATTGTGTT GGTGGCAAAA GGGGGAGAGC CCAAACAGCT 
CCAACAAAAA CTTCCCCCAG AAATGCAAAG AAGCATGATG AGTTATGGCA TGATGGAGTG 
TGCCCGTCAG TAGCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATC 
ACCTTTGAAG ACCCATCCTT AGATGTAATA CTTCTTTTAA GAGTTTTACA TGCCATCAGT 
CGATATTGGT ATTACTTGTA TGATAATGCG ATGTGCAAGG AGATTATTCC AACTAGTGAA 
TTTATTAACA GTAAATTAAC AGCAAAAGCA AATAGGCAGC TTCAAGACCC TCTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTCACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGACAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGGGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA CAGCAGAGTT 
GCGCCTAGAT TGGATAGAAA AAAACGTACT GTGAACAGAG AGGAGCTACT GAAACAAGCT 
GAGTCTGTGA TGCAGGACCT TGGCAGCTCT CGGGCCATGT TAGAAATCCA ATATGAAAAT 
GAGGTTGGTA CAGGTCTGGG GCCAACACTG GAGTTTTATG CACTTGTTTC TCAGGAACTA 
CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCTA AGCCAGCTCA CATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCTA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCTCTTGG CCTGCCCTTT 
TATAAGTGGA TGCTGCGGCA GGAAACTTCA TTGACATCTC ATGATTTATT TGACATTGAT 
CCTGTTGTAG CCAGATCAGT ATATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTG 
GAGCAGGATA AATCCCAGAC CAAAGAGAGT CTACAATATG CATTGGAAAC TCTTACGATG 
AATGGCTGTT CAGTGGAAGA TCTGGGATTG GATTTTACGC TGCCTGGGTT TCCCAACATT 
GAATTGAAGA AAGGAGGGAA GGATATTCCA GTTACTATCC ACAATTTGGA AGAGTACCTG 
AGATTGGTGA TATTCTGGGC ACTAAATGAA GGTGTTTGTC GACAGTTTGA CTCATTCAGA 
GATGGATTTG AATCGGTCTT CCCGCTCTGC CATCTTCAGT ACTTCTACCC AGAAGAGCTG 
GACCAGCTCC TGTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACATT GATGGAATGC 
TGCAGGCCTG ATCATGGCTA TACTCATGAC AGTCGGGCTG TGAAGTTCTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA 
CGATTGCCAG TTGGAGGATT TCGGAGTTTA AATCCACCTC TGACAATTGT GCGGAAGACA 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTACCATCAG TAATGACTTG TGTGAACTAT 
CTTAAATTGC CGGACTACTC GAGCATTGAC ATAATGCGGG ACAAACTCTT GATAGCAGCA 
AGAGAAGGCC AACAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496416.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496479.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X15, mRNA.

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CloneID	OMu27712
Clone ID Related Accession (Same CDS sequence)	XM_006496416.3 , XM_006496416.4
Accession Version	XM_006496416.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496416.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAAG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAATCC CTTAGCCAAT 
TCTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTAATGAAA 
GAAGATCCGG AACTGGCTAA GTCTTTCATT AAGACCTTGT TTGGTGTTCT CTATGAAGTG 
TACAGCTCCT CAGCAGGCCC TGCGGTCAGA CACAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CTGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CAATGCTATC CAGCCAAGAC CTGAAGATAG TAGTTGGAGC TCTTCAGATG 
GCAGAAATCC TAATGCAAAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG GAGAGAAGGT 
GTGATGCATC AAGTCAAACA TTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCCCCCAAG 
GCATGTACAA ATGGGTCAGG ATCCCTGGGA TCTACAACAC CAGCCAGCAG TGGGACTGCA 
ACGGCAGCAA CCAACGCCTC TGCTGATCTG GGGTCACCTA GCTTGCAGCA TAGCAGAGAT 
GATTCTTTGG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTCAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAATAT TCCCCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCCTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAATAT CGAACCAGCA 
CGAACTGCAG GAGTTAGTGG TCTTGCCAGG GCAGCCTCAA AAGACACCAT ATCCAATAAT 
AGAGAAAAAA TTAAAGGCTG GATTAAGGAG CAGGCACATA AATTTGTTGA GCGTTATTTT 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCCTTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCTTCA TTTGAAATCC AACATAGTGG GTTTGTGAAA 
CAACTGTTGC TGTATTTGAC ATCTAAGAAT GAAAAGGATG CTGTCGGCAG AGAGATCCGG 
TTAAAACGTT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCTGTTGGA 
AGAGTAGAAC CAGTGGGCCA TGCACCTTTG CTGGCATTGG TTCACAAGAT GAACAACTGT 
CTCAGCCAAA TGGAGCAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG GAACGGAGCT 
GGAGGAAGCT TTTCTCTCAA CAGAGGATCA CAAGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGTC AGTTACAAAG ACACCCAGAC TGTGCAAATG TGAAGCAGTG GAAAGGCGGG 
CCTGTCAAGA TTGATCCCCT GGCTCTGGTG CAAGCTATTG AGAGATACCT TGTGGTTAGA 
GGATATGGAA GAGTAAGAGA AGATGATGAA GATAGTGATG ATGATGGGTC AGATGAAGAA 
ATAGATGAGT CTTTGGCTGC TCAATTCTTA AATTCGGGAA ATGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT ACTACCATAT AACATGACCG TGTATCAGGC AGTGCGGCAA 
TTCAGTGTAC AGGCTGAAGA TGAAAGAGAA TCTACTGATG ATGAGAGCAA TCCTCTGGGA 
AGAGCTGGAA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG GGAGGATGAA 
GAAAGTACTA AAGATTGTGT TGGTGGCAAA AGGGGGAGAG CCCAAACAGC TCCAACAAAA 
ACTTCCCCCA GAAATGCAAA GAAGCATGAT GAGTTATGGC ATGATGGAGT GTGCCCGTCA 
GTAGCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT CACCTTTGAA 
GACCCATCCT TAGATGTAAT ACTTCTTTTA AGAGTTTTAC ATGCCATCAG TCGATATTGG 
TATTACTTGT ATGATAATGC GATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAATTAA CAGCAAAAGC AAATAGGCAG CTTCAAGACC CTCTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTCAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGACAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGGGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ACAGCAGAGT TGCGCCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTAC TGAAACAAGC TGAGTCTGTG 
ATGCAGGACC TTGGCAGCTC TCGGGCCATG TTAGAAATCC AATATGAAAA TGAGGTTGGT 
ACAGGTCTGG GGCCAACACT GGAGTTTTAT GCACTTGTTT CTCAGGAACT ACAGAGAGCT 
GACTTGTGTC TCTGGAGGGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ACATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCT 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCTCTTG GCCTGCCCTT TTATAAGTGG 
ATGCTGCGGC AGGAAACTTC ATTGACATCT CATGATTTAT TTGACATTGA TCCTGTTGTA 
GCCAGATCAG TATATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT GGAGCAGGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTGGAAA CTCTTACGAT GAATGGCTGT 
TCAGTGGAAG ATCTGGGATT GGATTTTACG CTGCCTGGGT TTCCCAACAT TGAATTGAAG 
AAAGGAGGGA AGGATATTCC AGTTACTATC CACAATTTGG AAGAGTACCT GAGATTGGTG 
ATATTCTGGG CACTAAATGA AGGTGTTTGT CGACAGTTTG ACTCATTCAG AGATGGATTT 
GAATCGGTCT TCCCGCTCTG CCATCTTCAG TACTTCTACC CAGAAGAGCT GGACCAGCTC 
CTGTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAT TGATGGAATG CTGCAGGCCT 
GATCATGGCT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CTGGTAGCCC ACGATTGCCA 
GTTGGAGGAT TTCGGAGTTT AAATCCACCT CTGACAATTG TGCGGAAGAC ATTTGAATCA 
ACAGAAAACC CAGATGACTT CTTACCATCA GTAATGACTT GTGTGAACTA TCTTAAATTG 
CCGGACTACT CGAGCATTGA CATAATGCGG GACAAACTCT TGATAGCAGC AAGAGAAGGC 
CAACAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496479.1
CDS	363..6566
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496416.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496416.4

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAAG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAATCC CTTAGCCAAT 
TCTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTAATGAAA 
GAAGATCCGG AACTGGCTAA GTCTTTCATT AAGACCTTGT TTGGTGTTCT CTATGAAGTG 
TACAGCTCCT CAGCAGGCCC TGCGGTCAGA CACAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CTGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CAATGCTATC CAGCCAAGAC CTGAAGATAG TAGTTGGAGC TCTTCAGATG 
GCAGAAATCC TAATGCAAAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG GAGAGAAGGT 
GTGATGCATC AAGTCAAACA TTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCCCCCAAG 
GCATGTACAA ATGGGTCAGG ATCCCTGGGA TCTACAACAC CAGCCAGCAG TGGGACTGCA 
ACGGCAGCAA CCAACGCCTC TGCTGATCTG GGGTCACCTA GCTTGCAGCA TAGCAGAGAT 
GATTCTTTGG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTCAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAATAT TCCCCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCCTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAATAT CGAACCAGCA 
CGAACTGCAG GAGTTAGTGG TCTTGCCAGG GCAGCCTCAA AAGACACCAT ATCCAATAAT 
AGAGAAAAAA TTAAAGGCTG GATTAAGGAG CAGGCACATA AATTTGTTGA GCGTTATTTT 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCCTTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCTTCA TTTGAAATCC AACATAGTGG GTTTGTGAAA 
CAACTGTTGC TGTATTTGAC ATCTAAGAAT GAAAAGGATG CTGTCGGCAG AGAGATCCGG 
TTAAAACGTT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCTGTTGGA 
AGAGTAGAAC CAGTGGGCCA TGCACCTTTG CTGGCATTGG TTCACAAGAT GAACAACTGT 
CTCAGCCAAA TGGAGCAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG GAACGGAGCT 
GGAGGAAGCT TTTCTCTCAA CAGAGGATCA CAAGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGTC AGTTACAAAG ACACCCAGAC TGTGCAAATG TGAAGCAGTG GAAAGGCGGG 
CCTGTCAAGA TTGATCCCCT GGCTCTGGTG CAAGCTATTG AGAGATACCT TGTGGTTAGA 
GGATATGGAA GAGTAAGAGA AGATGATGAA GATAGTGATG ATGATGGGTC AGATGAAGAA 
ATAGATGAGT CTTTGGCTGC TCAATTCTTA AATTCGGGAA ATGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT ACTACCATAT AACATGACCG TGTATCAGGC AGTGCGGCAA 
TTCAGTGTAC AGGCTGAAGA TGAAAGAGAA TCTACTGATG ATGAGAGCAA TCCTCTGGGA 
AGAGCTGGAA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG GGAGGATGAA 
GAAAGTACTA AAGATTGTGT TGGTGGCAAA AGGGGGAGAG CCCAAACAGC TCCAACAAAA 
ACTTCCCCCA GAAATGCAAA GAAGCATGAT GAGTTATGGC ATGATGGAGT GTGCCCGTCA 
GTAGCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT CACCTTTGAA 
GACCCATCCT TAGATGTAAT ACTTCTTTTA AGAGTTTTAC ATGCCATCAG TCGATATTGG 
TATTACTTGT ATGATAATGC GATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAATTAA CAGCAAAAGC AAATAGGCAG CTTCAAGACC CTCTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTCAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGACAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGGGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ACAGCAGAGT TGCGCCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTAC TGAAACAAGC TGAGTCTGTG 
ATGCAGGACC TTGGCAGCTC TCGGGCCATG TTAGAAATCC AATATGAAAA TGAGGTTGGT 
ACAGGTCTGG GGCCAACACT GGAGTTTTAT GCACTTGTTT CTCAGGAACT ACAGAGAGCT 
GACTTGTGTC TCTGGAGGGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ACATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCT 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCTCTTG GCCTGCCCTT TTATAAGTGG 
ATGCTGCGGC AGGAAACTTC ATTGACATCT CATGATTTAT TTGACATTGA TCCTGTTGTA 
GCCAGATCAG TATATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT GGAGCAGGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTGGAAA CTCTTACGAT GAATGGCTGT 
TCAGTGGAAG ATCTGGGATT GGATTTTACG CTGCCTGGGT TTCCCAACAT TGAATTGAAG 
AAAGGAGGGA AGGATATTCC AGTTACTATC CACAATTTGG AAGAGTACCT GAGATTGGTG 
ATATTCTGGG CACTAAATGA AGGTGTTTGT CGACAGTTTG ACTCATTCAG AGATGGATTT 
GAATCGGTCT TCCCGCTCTG CCATCTTCAG TACTTCTACC CAGAAGAGCT GGACCAGCTC 
CTGTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAT TGATGGAATG CTGCAGGCCT 
GATCATGGCT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CTGGTAGCCC ACGATTGCCA 
GTTGGAGGAT TTCGGAGTTT AAATCCACCT CTGACAATTG TGCGGAAGAC ATTTGAATCA 
ACAGAAAACC CAGATGACTT CTTACCATCA GTAATGACTT GTGTGAACTA TCTTAAATTG 
CCGGACTACT CGAGCATTGA CATAATGCGG GACAAACTCT TGATAGCAGC AAGAGAAGGC 
CAACAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496422.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496485.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X25, mRNA.

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CloneID	OMu27718
Clone ID Related Accession (Same CDS sequence)	XM_006496422.3 , XM_006496422.4
Accession Version	XM_006496422.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496422.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAAGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAATCCC TTAGCCAATT CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTAATGAAAG AAGATCCGGA ACTGGCTAAG 
TCTTTCATTA AGACCTTGTT TGGTGTTCTC TATGAAGTGT ACAGCTCCTC AGCAGGCCCT 
GCGGTCAGAC ACAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC TGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC AATGCTATCC 
AGCCAAGACC TGAAGATAGT AGTTGGAGCT CTTCAGATGG CAGAAATCCT AATGCAAAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGG AGAGAAGGTG TGATGCATCA AGTCAAACAT 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCCCCCAAGG CATGTACAAA TGGGTCAGGA 
TCCCTGGGAT CTACAACACC AGCCAGCAGT GGGACTGCAA CGGCAGCAAC CAACGCCTCT 
GCTGATCTGG GGTCACCTAG CTTGCAGCAT AGCAGAGATG ATTCTTTGGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTCAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAATATT CCCCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCCTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAATATC GAACCAGCAC GAACTGCAGG AGTTAGTGGT 
CTTGCCAGGG CAGCCTCAAA AGACACCATA TCCAATAATA GAGAAAAAAT TAAAGGCTGG 
ATTAAGGAGC AGGCACATAA ATTTGTTGAG CGTTATTTTA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CCTTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCTTCAT TTGAAATCCA ACATAGTGGG TTTGTGAAAC AACTGTTGCT GTATTTGACA 
TCTAAGAATG AAAAGGATGC TGTCGGCAGA GAGATCCGGT TAAAACGTTT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCTGTTGGAA GAGTAGAACC AGTGGGCCAT 
GCACCTTTGC TGGCATTGGT TCACAAGATG AACAACTGTC TCAGCCAAAT GGAGCAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGG AACGGAGCTG GAGGAAGCTT TTCTCTCAAC 
AGAGGATCAC AAGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGTCA GTTACAAAGA 
CACCCAGACT GTGCAAATGT GAAGCAGTGG AAAGGCGGGC CTGTCAAGAT TGATCCCCTG 
GCTCTGGTGC AAGCTATTGA GAGATACCTT GTGGTTAGAG GATATGGAAG AGTAAGAGAA 
GATGATGAAG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAGTC TTTGGCTGCT 
CAATTCTTAA ATTCGGGAAA TGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTA 
CTACCATATA ACATGACCGT GTATCAGGCA GTGCGGCAAT TCAGTGTACA GGCTGAAGAT 
GAAAGAGAAT CTACTGATGA TGAGAGCAAT CCTCTGGGAA GAGCTGGAAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGG GAGGATGAAG AAAGTACTAA AGATTGTGTT 
GGTGGCAAAA GGGGGAGAGC CCAAACAGCT CCAACAAAAA CTTCCCCCAG AAATGCAAAG 
AAGCATGATG AGTTATGGCA TGATGGAGTG TGCCCGTCAG TAGCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATC ACCTTTGAAG ACCCATCCTT AGATGTAATA 
CTTCTTTTAA GAGTTTTACA TGCCATCAGT CGATATTGGT ATTACTTGTA TGATAATGCG 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAATTAAC AGCAAAAGCA 
AATAGGCAGC TTCAAGACCC TCTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTCACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGACAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGGGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA CAGCAGAGTT GCGCCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTACT GAAACAAGCT GAGTCTGTGA TGCAGGACCT TGGCAGCTCT 
CGGGCCATGT TAGAAATCCA ATATGAAAAT GAGGTTGGTA CAGGTCTGGG GCCAACACTG 
GAGTTTTATG CACTTGTTTC TCAGGAACTA CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA CATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCTA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCTCTTGG CCTGCCCTTT TATAAGTGGA TGCTGCGGCA GGAAACTTCA 
TTGACATCTC ATGATTTATT TGACATTGAT CCTGTTGTAG CCAGATCAGT ATATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTG GAGCAGGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTGGAAAC TCTTACGATG AATGGCTGTT CAGTGGAAGA TCTGGGATTG 
GATTTTACGC TGCCTGGGTT TCCCAACATT GAATTGAAGA AAGGAGGGAA GGATATTCCA 
GTTACTATCC ACAATTTGGA AGAGTACCTG AGATTGGTGA TATTCTGGGC ACTAAATGAA 
GGTGTTTGTC GACAGTTTGA CTCATTCAGA GATGGATTTG AATCGGTCTT CCCGCTCTGC 
CATCTTCAGT ACTTCTACCC AGAAGAGCTG GACCAGCTCC TGTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACATT GATGGAATGC TGCAGGCCTG ATCATGGCTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA CGATTGCCAG TTGGAGGATT TCGGAGTTTA 
AATCCACCTC TGACAATTGT GCGGAAGACA TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTACCATCAG TAATGACTTG TGTGAACTAT CTTAAATTGC CGGACTACTC GAGCATTGAC 
ATAATGCGGG ACAAACTCTT GATAGCAGCA AGAGAAGGCC AACAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496485.1
CDS	363..6485
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496422.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496422.4

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAAGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAATCCC TTAGCCAATT CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTAATGAAAG AAGATCCGGA ACTGGCTAAG 
TCTTTCATTA AGACCTTGTT TGGTGTTCTC TATGAAGTGT ACAGCTCCTC AGCAGGCCCT 
GCGGTCAGAC ACAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC TGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC AATGCTATCC 
AGCCAAGACC TGAAGATAGT AGTTGGAGCT CTTCAGATGG CAGAAATCCT AATGCAAAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGG AGAGAAGGTG TGATGCATCA AGTCAAACAT 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCCCCCAAGG CATGTACAAA TGGGTCAGGA 
TCCCTGGGAT CTACAACACC AGCCAGCAGT GGGACTGCAA CGGCAGCAAC CAACGCCTCT 
GCTGATCTGG GGTCACCTAG CTTGCAGCAT AGCAGAGATG ATTCTTTGGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTCAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAATATT CCCCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCCTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAATATC GAACCAGCAC GAACTGCAGG AGTTAGTGGT 
CTTGCCAGGG CAGCCTCAAA AGACACCATA TCCAATAATA GAGAAAAAAT TAAAGGCTGG 
ATTAAGGAGC AGGCACATAA ATTTGTTGAG CGTTATTTTA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CCTTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCTTCAT TTGAAATCCA ACATAGTGGG TTTGTGAAAC AACTGTTGCT GTATTTGACA 
TCTAAGAATG AAAAGGATGC TGTCGGCAGA GAGATCCGGT TAAAACGTTT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCTGTTGGAA GAGTAGAACC AGTGGGCCAT 
GCACCTTTGC TGGCATTGGT TCACAAGATG AACAACTGTC TCAGCCAAAT GGAGCAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGG AACGGAGCTG GAGGAAGCTT TTCTCTCAAC 
AGAGGATCAC AAGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGTCA GTTACAAAGA 
CACCCAGACT GTGCAAATGT GAAGCAGTGG AAAGGCGGGC CTGTCAAGAT TGATCCCCTG 
GCTCTGGTGC AAGCTATTGA GAGATACCTT GTGGTTAGAG GATATGGAAG AGTAAGAGAA 
GATGATGAAG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAGTC TTTGGCTGCT 
CAATTCTTAA ATTCGGGAAA TGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTA 
CTACCATATA ACATGACCGT GTATCAGGCA GTGCGGCAAT TCAGTGTACA GGCTGAAGAT 
GAAAGAGAAT CTACTGATGA TGAGAGCAAT CCTCTGGGAA GAGCTGGAAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGG GAGGATGAAG AAAGTACTAA AGATTGTGTT 
GGTGGCAAAA GGGGGAGAGC CCAAACAGCT CCAACAAAAA CTTCCCCCAG AAATGCAAAG 
AAGCATGATG AGTTATGGCA TGATGGAGTG TGCCCGTCAG TAGCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATC ACCTTTGAAG ACCCATCCTT AGATGTAATA 
CTTCTTTTAA GAGTTTTACA TGCCATCAGT CGATATTGGT ATTACTTGTA TGATAATGCG 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAATTAAC AGCAAAAGCA 
AATAGGCAGC TTCAAGACCC TCTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTCACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGACAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGGGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA CAGCAGAGTT GCGCCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTACT GAAACAAGCT GAGTCTGTGA TGCAGGACCT TGGCAGCTCT 
CGGGCCATGT TAGAAATCCA ATATGAAAAT GAGGTTGGTA CAGGTCTGGG GCCAACACTG 
GAGTTTTATG CACTTGTTTC TCAGGAACTA CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA CATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCTA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCTCTTGG CCTGCCCTTT TATAAGTGGA TGCTGCGGCA GGAAACTTCA 
TTGACATCTC ATGATTTATT TGACATTGAT CCTGTTGTAG CCAGATCAGT ATATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTG GAGCAGGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTGGAAAC TCTTACGATG AATGGCTGTT CAGTGGAAGA TCTGGGATTG 
GATTTTACGC TGCCTGGGTT TCCCAACATT GAATTGAAGA AAGGAGGGAA GGATATTCCA 
GTTACTATCC ACAATTTGGA AGAGTACCTG AGATTGGTGA TATTCTGGGC ACTAAATGAA 
GGTGTTTGTC GACAGTTTGA CTCATTCAGA GATGGATTTG AATCGGTCTT CCCGCTCTGC 
CATCTTCAGT ACTTCTACCC AGAAGAGCTG GACCAGCTCC TGTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACATT GATGGAATGC TGCAGGCCTG ATCATGGCTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA CGATTGCCAG TTGGAGGATT TCGGAGTTTA 
AATCCACCTC TGACAATTGT GCGGAAGACA TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTACCATCAG TAATGACTTG TGTGAACTAT CTTAAATTGC CGGACTACTC GAGCATTGAC 
ATAATGCGGG ACAAACTCTT GATAGCAGCA AGAGAAGGCC AACAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496427.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496490.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X39, mRNA.

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CloneID	OMu27720
Clone ID Related Accession (Same CDS sequence)	XM_006496427.3 , XM_006496427.2
Accession Version	XM_006496427.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAAGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ATCCCTTAGC CAATTCTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAT 
CCGGAACTGG CTAAGTCTTT CATTAAGACC TTGTTTGGTG TTCTCTATGA AGTGTACAGC 
TCCTCAGCAG GCCCTGCGGT CAGACACAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCTGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCAATGC TATCCAGCCA AGACCTGAAG ATAGTAGTTG GAGCTCTTCA GATGGCAGAA 
ATCCTAATGC AAAAGTTACC TGATATTTTT AGTGTTTACT TCAGGAGAGA AGGTGTGATG 
CATCAAGTCA AACATTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCCCC CAAGGCATGT 
ACAAATGGGT CAGGATCCCT GGGATCTACA ACACCAGCCA GCAGTGGGAC TGCAACGGCA 
GCAACCAACG CCTCTGCTGA TCTGGGGTCA CCTAGCTTGC AGCATAGCAG AGATGATTCT 
TTGGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTCA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA ATATTCCCCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCCT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ATATCGAACC AGCACGAACT 
GCAGGAGTTA GTGGTCTTGC CAGGGCAGCC TCAAAAGACA CCATATCCAA TAATAGAGAA 
AAAATTAAAG GCTGGATTAA GGAGCAGGCA CATAAATTTG TTGAGCGTTA TTTTAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCCTTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC TTCATTTGAA ATCCAACATA GTGGGTTTGT GAAACAACTG 
TTGCTGTATT TGACATCTAA GAATGAAAAG GATGCTGTCG GCAGAGAGAT CCGGTTAAAA 
CGTTTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCTGT TGGAAGAGTA 
GAACCAGTGG GCCATGCACC TTTGCTGGCA TTGGTTCACA AGATGAACAA CTGTCTCAGC 
CAAATGGAGC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGGAACGG AGCTGGAGGA 
AGCTTTTCTC TCAACAGAGG ATCACAAGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGTCAGTTAC AAAGACACCC AGACTGTGCA AATGTGAAGC AGTGGAAAGG CGGGCCTGTC 
AAGATTGATC CCCTGGCTCT GGTGCAAGCT ATTGAGAGAT ACCTTGTGGT TAGAGGTATT 
TTCCGATATG GAAGAGTAAG AGAAGATGAT GAAGATAGTG ATGATGATGG GTCAGATGAA 
GAAATAGATG AGTCTTTGGC TGCTCAATTC TTAAATTCGG GAAATGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTACTACCA TATAACATGA CCGTGTATCA GGCAGTGCGG 
CAATTCAGTG TACAGGCTGA AGATGAAAGA GAATCTACTG ATGATGAGAG CAATCCTCTG 
GGAAGAGCTG GAATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGGGAGGAT 
GAAGAAAGTA CTAAAGATTG TGTTGGTGGC AAAAGGGGGA GAGCCCAAAC AGCTCCAACA 
AAAACTTCCC CCAGAAATGC AAAGAAGCAT GATGAGTTAT GGCATGATGG AGTGTGCCCG 
TCAGTAGCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATCACCTTT 
GAAGACCCAT CCTTAGATGT AATACTTCTT TTAAGAGTTT TACATGCCAT CAGTCGATAT 
TGGTATTACT TGTATGATAA TGCGATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAAT TAACAGCAAA AGCAAATAGG CAGCTTCAAG ACCCTCTAGT AATCATGACA 
GGAAACATCC CAACATGGCT CACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGAC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGGGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGACAGCAG AGTTGCGCCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TACTGAAACA AGCTGAGTCT 
GTGATGCAGG ACCTTGGCAG CTCTCGGGCC ATGTTAGAAA TCCAATATGA AAATGAGGTT 
GGTACAGGTC TGGGGCCAAC ACTGGAGTTT TATGCACTTG TTTCTCAGGA ACTACAGAGA 
GCTGACTTGT GTCTCTGGAG GGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCACATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCTAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCTC TTGGCCTGCC CTTTTATAAG 
TGGATGCTGC GGCAGGAAAC TTCATTGACA TCTCATGATT TATTTGACAT TGATCCTGTT 
GTAGCCAGAT CAGTATATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTGGAGCAG 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTGG AAACTCTTAC GATGAATGGC 
TGTTCAGTGG AAGATCTGGG ATTGGATTTT ACGCTGCCTG GGTTTCCCAA CATTGAATTG 
AAGAAAGGAG GGAAGGATAT TCCAGTTACT ATCCACAATT TGGAAGAGTA CCTGAGATTG 
GTGATATTCT GGGCACTAAA TGAAGGTGTT TGTCGACAGT TTGACTCATT CAGAGATGGA 
TTTGAATCGG TCTTCCCGCT CTGCCATCTT CAGTACTTCT ACCCAGAAGA GCTGGACCAG 
CTCCTGTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CATTGATGGA ATGCTGCAGG 
CCTGATCATG GCTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACTGGTAG CCCACGATTG 
CCAGTTGGAG GATTTCGGAG TTTAAATCCA CCTCTGACAA TTGTGCGGAA GACATTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTACCA TCAGTAATGA CTTGTGTGAA CTATCTTAAA 
TTGCCGGACT ACTCGAGCAT TGACATAATG CGGGACAAAC TCTTGATAGC AGCAAGAGAA 
GGCCAACAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496490.1
CDS	363..6449
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496427.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496427.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAAGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ATCCCTTAGC CAATTCTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAT 
CCGGAACTGG CTAAGTCTTT CATTAAGACC TTGTTTGGTG TTCTCTATGA AGTGTACAGC 
TCCTCAGCAG GCCCTGCGGT CAGACACAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCTGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCAATGC TATCCAGCCA AGACCTGAAG ATAGTAGTTG GAGCTCTTCA GATGGCAGAA 
ATCCTAATGC AAAAGTTACC TGATATTTTT AGTGTTTACT TCAGGAGAGA AGGTGTGATG 
CATCAAGTCA AACATTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCCCC CAAGGCATGT 
ACAAATGGGT CAGGATCCCT GGGATCTACA ACACCAGCCA GCAGTGGGAC TGCAACGGCA 
GCAACCAACG CCTCTGCTGA TCTGGGGTCA CCTAGCTTGC AGCATAGCAG AGATGATTCT 
TTGGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTCA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA ATATTCCCCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCCT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ATATCGAACC AGCACGAACT 
GCAGGAGTTA GTGGTCTTGC CAGGGCAGCC TCAAAAGACA CCATATCCAA TAATAGAGAA 
AAAATTAAAG GCTGGATTAA GGAGCAGGCA CATAAATTTG TTGAGCGTTA TTTTAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCCTTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC TTCATTTGAA ATCCAACATA GTGGGTTTGT GAAACAACTG 
TTGCTGTATT TGACATCTAA GAATGAAAAG GATGCTGTCG GCAGAGAGAT CCGGTTAAAA 
CGTTTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCTGT TGGAAGAGTA 
GAACCAGTGG GCCATGCACC TTTGCTGGCA TTGGTTCACA AGATGAACAA CTGTCTCAGC 
CAAATGGAGC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGGAACGG AGCTGGAGGA 
AGCTTTTCTC TCAACAGAGG ATCACAAGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGTCAGTTAC AAAGACACCC AGACTGTGCA AATGTGAAGC AGTGGAAAGG CGGGCCTGTC 
AAGATTGATC CCCTGGCTCT GGTGCAAGCT ATTGAGAGAT ACCTTGTGGT TAGAGGTATT 
TTCCGATATG GAAGAGTAAG AGAAGATGAT GAAGATAGTG ATGATGATGG GTCAGATGAA 
GAAATAGATG AGTCTTTGGC TGCTCAATTC TTAAATTCGG GAAATGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTACTACCA TATAACATGA CCGTGTATCA GGCAGTGCGG 
CAATTCAGTG TACAGGCTGA AGATGAAAGA GAATCTACTG ATGATGAGAG CAATCCTCTG 
GGAAGAGCTG GAATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGGGAGGAT 
GAAGAAAGTA CTAAAGATTG TGTTGGTGGC AAAAGGGGGA GAGCCCAAAC AGCTCCAACA 
AAAACTTCCC CCAGAAATGC AAAGAAGCAT GATGAGTTAT GGCATGATGG AGTGTGCCCG 
TCAGTAGCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATCACCTTT 
GAAGACCCAT CCTTAGATGT AATACTTCTT TTAAGAGTTT TACATGCCAT CAGTCGATAT 
TGGTATTACT TGTATGATAA TGCGATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAAT TAACAGCAAA AGCAAATAGG CAGCTTCAAG ACCCTCTAGT AATCATGACA 
GGAAACATCC CAACATGGCT CACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGAC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGGGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGACAGCAG AGTTGCGCCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TACTGAAACA AGCTGAGTCT 
GTGATGCAGG ACCTTGGCAG CTCTCGGGCC ATGTTAGAAA TCCAATATGA AAATGAGGTT 
GGTACAGGTC TGGGGCCAAC ACTGGAGTTT TATGCACTTG TTTCTCAGGA ACTACAGAGA 
GCTGACTTGT GTCTCTGGAG GGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCACATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCTAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCTC TTGGCCTGCC CTTTTATAAG 
TGGATGCTGC GGCAGGAAAC TTCATTGACA TCTCATGATT TATTTGACAT TGATCCTGTT 
GTAGCCAGAT CAGTATATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTGGAGCAG 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTGG AAACTCTTAC GATGAATGGC 
TGTTCAGTGG AAGATCTGGG ATTGGATTTT ACGCTGCCTG GGTTTCCCAA CATTGAATTG 
AAGAAAGGAG GGAAGGATAT TCCAGTTACT ATCCACAATT TGGAAGAGTA CCTGAGATTG 
GTGATATTCT GGGCACTAAA TGAAGGTGTT TGTCGACAGT TTGACTCATT CAGAGATGGA 
TTTGAATCGG TCTTCCCGCT CTGCCATCTT CAGTACTTCT ACCCAGAAGA GCTGGACCAG 
CTCCTGTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CATTGATGGA ATGCTGCAGG 
CCTGATCATG GCTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACTGGTAG CCCACGATTG 
CCAGTTGGAG GATTTCGGAG TTTAAATCCA CCTCTGACAA TTGTGCGGAA GACATTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTACCA TCAGTAATGA CTTGTGTGAA CTATCTTAAA 
TTGCCGGACT ACTCGAGCAT TGACATAATG CGGGACAAAC TCTTGATAGC AGCAAGAGAA 
GGCCAACAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238654.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236956.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X1, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238654.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238654.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236956.1
CDS	363..6587
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238654.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238654.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238655.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236957.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X2, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238655.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238655.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236957.1
CDS	133..6357
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238655.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238655.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238657.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236959.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X4, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238657.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236959.1
CDS	257..6481
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238657.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238657.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238660.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236962.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X7, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238660.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238660.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236962.1
CDS	74..6298
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238660.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238660.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238658.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236960.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X5, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238658.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238658.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236960.1
CDS	135..6359
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238658.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238658.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238656.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236958.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X3, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238656.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238656.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236958.1
CDS	174..6398
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238656.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238656.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030248874.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104734.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X8, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_030248874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104734.1
CDS	2549..8773
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030248874.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248874.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238661.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236963.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X9, mRNA.

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CloneID	OMu67885
Clone ID Related Accession (Same CDS sequence)	XM_011238661.2 , XM_011238661.3
Accession Version	XM_011238661.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAAGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAATCCC 
TTAGCCAATT CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTAATGAAAG AAGATCCGGA ACTGGCTAAG TCTTTCATTA AGACCTTGTT TGGTGTTCTC 
TATGAAGTGT ACAGCTCCTC AGCAGGCCCT GCGGTCAGAC ACAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTTGC TGATGCTGAA CTTCTGAAGG ATGTTCTGAA AAATCATGCT 
GTTTCAAGTC ACATTGCTTC AATGCTATCC AGCCAAGACC TGAAGATAGT AGTTGGAGCT 
CTTCAGATGG CAGAAATCCT AATGCAAAAG TTACCTGATA TTTTTAGTGT TTACTTCAGG 
AGAGAAGGTG TGATGCATCA AGTCAAACAT TTAGCAGAAT CAGAGTCTTT GTTGACAAGT 
CCCCCCAAGG CATGTACAAA TGGGTCAGGA TCCCTGGGAT CTACAACACC AGCCAGCAGT 
GGGACTGCAA CGGCAGCAAC CAACGCCTCT GCTGATCTGG GGTCACCTAG CTTGCAGCAT 
AGCAGAGATG ATTCTTTGGA TCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTCAAGAGA 
AAACGACTGC CAAAACGAGG GCCAAGAAGG CCAAAATATT CCCCTCCAAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACC ACTACTCAGT CACCTAAATC TTCCTTCCTG 
GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG TTAAGCGCAC AGTCCAACAG CAACAATATC 
GAACCAGCAC GAACTGCAGG AGTTAGTGGT CTTGCCAGGG CAGCCTCAAA AGACACCATA 
TCCAATAATA GAGAAAAAAT TAAAGGCTGG ATTAAGGAGC AGGCACATAA ATTTGTTGAG 
CGTTATTTTA GTTCTGAGAA TATGGATGGA AGCAACCCTG CCTTGAATGT CCTTCAGAGA 
CTTTGTGCTG CAACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCAGAT GTTTCTTCAT TTGAAATCCA ACATAGTGGG 
TTTGTGAAAC AACTGTTGCT GTATTTGACA TCTAAGAATG AAAAGGATGC TGTCGGCAGA 
GAGATCCGGT TAAAACGTTT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCTGTTGGAA GAGTAGAACC AGTGGGCCAT GCACCTTTGC TGGCATTGGT TCACAAGATG 
AACAACTGTC TCAGCCAAAT GGAGCAATTT CCAGTCAAAG TACATGATTT CCCTAGTGGG 
AACGGAGCTG GAGGAAGCTT TTCTCTCAAC AGAGGATCAC AAGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGTCA GTTACAAAGA CACCCAGACT GTGCAAATGT GAAGCAGTGG 
AAAGGCGGGC CTGTCAAGAT TGATCCCCTG GCTCTGGTGC AAGCTATTGA GAGATACCTT 
GTGGTTAGAG GTATTTTCCG ATATGGAAGA GTAAGAGAAG ATGATGAAGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAGTCT TTGGCTGCTC AATTCTTAAA TTCGGGAAAT 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTAC TACCATATAA CATGACCGTG 
TATCAGGCAG TGCGGCAATT CAGTGTACAG GCTGAAGATG AAAGAGAATC TACTGATGAT 
GAGAGCAATC CTCTGGGAAG AGCTGGAATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGGG AGGATGAAGA AAGTACTAAA GATTGTGTTG GTGGCAAAAG GGGGAGAGCC 
CAAACAGCTC CAACAAAAAC TTCCCCCAGA AATGCAAAGA AGCATGATGA GTTATGGCAT 
GATGGAGTGT GCCCGTCAGT AGCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATCA CCTTTGAAGA CCCATCCTTA GATGTAATAC TTCTTTTAAG AGTTTTACAT 
GCCATCAGTC GATATTGGTA TTACTTGTAT GATAATGCGA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAATTAACA GCAAAAGCAA ATAGGCAGCT TCAAGACCCT 
CTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTCACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGACAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGGGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAC 
AGCAGAGTTG CGCCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTACTG 
AAACAAGCTG AGTCTGTGAT GCAGGACCTT GGCAGCTCTC GGGCCATGTT AGAAATCCAA 
TATGAAAATG AGGTTGGTAC AGGTCTGGGG CCAACACTGG AGTTTTATGC ACTTGTTTCT 
CAGGAACTAC AGAGAGCTGA CTTGTGTCTC TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAC ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCTAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCTCTTGGC 
CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG GAAACTTCAT TGACATCTCA TGATTTATTT 
GACATTGATC CTGTTGTAGC CAGATCAGTA TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTGG AGCAGGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTGGAAACT 
CTTACGATGA ATGGCTGTTC AGTGGAAGAT CTGGGATTGG ATTTTACGCT GCCTGGGTTT 
CCCAACATTG AATTGAAGAA AGGAGGGAAG GATATTCCAG TTACTATCCA CAATTTGGAA 
GAGTACCTGA GATTGGTGAT ATTCTGGGCA CTAAATGAAG GTGTTTGTCG ACAGTTTGAC 
TCATTCAGAG ATGGATTTGA ATCGGTCTTC CCGCTCTGCC ATCTTCAGTA CTTCTACCCA 
GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACATTG 
ATGGAATGCT GCAGGCCTGA TCATGGCTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACT 
GGTAGCCCAC GATTGCCAGT TGGAGGATTT CGGAGTTTAA ATCCACCTCT GACAATTGTG 
CGGAAGACAT TTGAATCAAC AGAAAACCCA GATGACTTCT TACCATCAGT AATGACTTGT 
GTGAACTATC TTAAATTGCC GGACTACTCG AGCATTGACA TAATGCGGGA CAAACTCTTG 
ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236963.1
CDS	363..6584
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238661.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238661.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAAGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAATCCC 
TTAGCCAATT CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTAATGAAAG AAGATCCGGA ACTGGCTAAG TCTTTCATTA AGACCTTGTT TGGTGTTCTC 
TATGAAGTGT ACAGCTCCTC AGCAGGCCCT GCGGTCAGAC ACAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTTGC TGATGCTGAA CTTCTGAAGG ATGTTCTGAA AAATCATGCT 
GTTTCAAGTC ACATTGCTTC AATGCTATCC AGCCAAGACC TGAAGATAGT AGTTGGAGCT 
CTTCAGATGG CAGAAATCCT AATGCAAAAG TTACCTGATA TTTTTAGTGT TTACTTCAGG 
AGAGAAGGTG TGATGCATCA AGTCAAACAT TTAGCAGAAT CAGAGTCTTT GTTGACAAGT 
CCCCCCAAGG CATGTACAAA TGGGTCAGGA TCCCTGGGAT CTACAACACC AGCCAGCAGT 
GGGACTGCAA CGGCAGCAAC CAACGCCTCT GCTGATCTGG GGTCACCTAG CTTGCAGCAT 
AGCAGAGATG ATTCTTTGGA TCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTCAAGAGA 
AAACGACTGC CAAAACGAGG GCCAAGAAGG CCAAAATATT CCCCTCCAAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACC ACTACTCAGT CACCTAAATC TTCCTTCCTG 
GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG TTAAGCGCAC AGTCCAACAG CAACAATATC 
GAACCAGCAC GAACTGCAGG AGTTAGTGGT CTTGCCAGGG CAGCCTCAAA AGACACCATA 
TCCAATAATA GAGAAAAAAT TAAAGGCTGG ATTAAGGAGC AGGCACATAA ATTTGTTGAG 
CGTTATTTTA GTTCTGAGAA TATGGATGGA AGCAACCCTG CCTTGAATGT CCTTCAGAGA 
CTTTGTGCTG CAACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCAGAT GTTTCTTCAT TTGAAATCCA ACATAGTGGG 
TTTGTGAAAC AACTGTTGCT GTATTTGACA TCTAAGAATG AAAAGGATGC TGTCGGCAGA 
GAGATCCGGT TAAAACGTTT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCTGTTGGAA GAGTAGAACC AGTGGGCCAT GCACCTTTGC TGGCATTGGT TCACAAGATG 
AACAACTGTC TCAGCCAAAT GGAGCAATTT CCAGTCAAAG TACATGATTT CCCTAGTGGG 
AACGGAGCTG GAGGAAGCTT TTCTCTCAAC AGAGGATCAC AAGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGTCA GTTACAAAGA CACCCAGACT GTGCAAATGT GAAGCAGTGG 
AAAGGCGGGC CTGTCAAGAT TGATCCCCTG GCTCTGGTGC AAGCTATTGA GAGATACCTT 
GTGGTTAGAG GTATTTTCCG ATATGGAAGA GTAAGAGAAG ATGATGAAGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAGTCT TTGGCTGCTC AATTCTTAAA TTCGGGAAAT 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTAC TACCATATAA CATGACCGTG 
TATCAGGCAG TGCGGCAATT CAGTGTACAG GCTGAAGATG AAAGAGAATC TACTGATGAT 
GAGAGCAATC CTCTGGGAAG AGCTGGAATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGGG AGGATGAAGA AAGTACTAAA GATTGTGTTG GTGGCAAAAG GGGGAGAGCC 
CAAACAGCTC CAACAAAAAC TTCCCCCAGA AATGCAAAGA AGCATGATGA GTTATGGCAT 
GATGGAGTGT GCCCGTCAGT AGCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATCA CCTTTGAAGA CCCATCCTTA GATGTAATAC TTCTTTTAAG AGTTTTACAT 
GCCATCAGTC GATATTGGTA TTACTTGTAT GATAATGCGA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAATTAACA GCAAAAGCAA ATAGGCAGCT TCAAGACCCT 
CTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTCACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGACAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGGGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAC 
AGCAGAGTTG CGCCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTACTG 
AAACAAGCTG AGTCTGTGAT GCAGGACCTT GGCAGCTCTC GGGCCATGTT AGAAATCCAA 
TATGAAAATG AGGTTGGTAC AGGTCTGGGG CCAACACTGG AGTTTTATGC ACTTGTTTCT 
CAGGAACTAC AGAGAGCTGA CTTGTGTCTC TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAC ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCTAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCTCTTGGC 
CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG GAAACTTCAT TGACATCTCA TGATTTATTT 
GACATTGATC CTGTTGTAGC CAGATCAGTA TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTGG AGCAGGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTGGAAACT 
CTTACGATGA ATGGCTGTTC AGTGGAAGAT CTGGGATTGG ATTTTACGCT GCCTGGGTTT 
CCCAACATTG AATTGAAGAA AGGAGGGAAG GATATTCCAG TTACTATCCA CAATTTGGAA 
GAGTACCTGA GATTGGTGAT ATTCTGGGCA CTAAATGAAG GTGTTTGTCG ACAGTTTGAC 
TCATTCAGAG ATGGATTTGA ATCGGTCTTC CCGCTCTGCC ATCTTCAGTA CTTCTACCCA 
GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACATTG 
ATGGAATGCT GCAGGCCTGA TCATGGCTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACT 
GGTAGCCCAC GATTGCCAGT TGGAGGATTT CGGAGTTTAA ATCCACCTCT GACAATTGTG 
CGGAAGACAT TTGAATCAAC AGAAAACCCA GATGACTTCT TACCATCAGT AATGACTTGT 
GTGAACTATC TTAAATTGCC GGACTACTCG AGCATTGACA TAATGCGGGA CAAACTCTTG 
ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238662.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236964.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X10, mRNA.

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CloneID	OMu67886
Clone ID Related Accession (Same CDS sequence)	XM_011238662.2 , XM_011238662.3
Accession Version	XM_011238662.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238662.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGATATGG AAGAGTAAGA GAAGATGATG AAGATAGTGA TGATGATGGG 
TCAGATGAAG AAATAGATGA GTCTTTGGCT GCTCAATTCT TAAATTCGGG AAATGTAAGG 
CACAGGCTAC AGTTTTATAT TGGAGAACAT TTACTACCAT ATAACATGAC CGTGTATCAG 
GCAGTGCGGC AATTCAGTGT ACAGGCTGAA GATGAAAGAG AATCTACTGA TGATGAGAGC 
AATCCTCTGG GAAGAGCTGG AATTTGGACA AAGACTCATA CAATATGGTA CAAACCTGTG 
AGGGAGGATG AAGAAAGTAC TAAAGATTGT GTTGGTGGCA AAAGGGGGAG AGCCCAAACA 
GCTCCAACAA AAACTTCCCC CAGAAATGCA AAGAAGCATG ATGAGTTATG GCATGATGGA 
GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATCACCTTTG AAGACCCATC CTTAGATGTA ATACTTCTTT TAAGAGTTTT ACATGCCATC 
AGTCGATATT GGTATTACTT GTATGATAAT GCGATGTGCA AGGAGATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAATT AACAGCAAAA GCAAATAGGC AGCTTCAAGA CCCTCTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTC ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGACA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGGGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGACAGCAGA 
GTTGCGCCTA GATTGGATAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT ACTGAAACAA 
GCTGAGTCTG TGATGCAGGA CCTTGGCAGC TCTCGGGCCA TGTTAGAAAT CCAATATGAA 
AATGAGGTTG GTACAGGTCT GGGGCCAACA CTGGAGTTTT ATGCACTTGT TTCTCAGGAA 
CTACAGAGAG CTGACTTGTG TCTCTGGAGG GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCACATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CTAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCTCT TGGCCTGCCC 
TTTTATAAGT GGATGCTGCG GCAGGAAACT TCATTGACAT CTCATGATTT ATTTGACATT 
GATCCTGTTG TAGCCAGATC AGTATATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTGGAGCAGG ATAAATCCCA GACCAAAGAG AGTCTACAAT ATGCATTGGA AACTCTTACG 
ATGAATGGCT GTTCAGTGGA AGATCTGGGA TTGGATTTTA CGCTGCCTGG GTTTCCCAAC 
ATTGAATTGA AGAAAGGAGG GAAGGATATT CCAGTTACTA TCCACAATTT GGAAGAGTAC 
CTGAGATTGG TGATATTCTG GGCACTAAAT GAAGGTGTTT GTCGACAGTT TGACTCATTC 
AGAGATGGAT TTGAATCGGT CTTCCCGCTC TGCCATCTTC AGTACTTCTA CCCAGAAGAG 
CTGGACCAGC TCCTGTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ATTGATGGAA 
TGCTGCAGGC CTGATCATGG CTATACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTGTTTC TCCAGTTTGT GACTGGTAGC 
CCACGATTGC CAGTTGGAGG ATTTCGGAGT TTAAATCCAC CTCTGACAAT TGTGCGGAAG 
ACATTTGAAT CAACAGAAAA CCCAGATGAC TTCTTACCAT CAGTAATGAC TTGTGTGAAC 
TATCTTAAAT TGCCGGACTA CTCGAGCATT GACATAATGC GGGACAAACT CTTGATAGCA 
GCAAGAGAAG GCCAACAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236964.1
CDS	363..6578
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238662.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238662.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGATATGG AAGAGTAAGA GAAGATGATG AAGATAGTGA TGATGATGGG 
TCAGATGAAG AAATAGATGA GTCTTTGGCT GCTCAATTCT TAAATTCGGG AAATGTAAGG 
CACAGGCTAC AGTTTTATAT TGGAGAACAT TTACTACCAT ATAACATGAC CGTGTATCAG 
GCAGTGCGGC AATTCAGTGT ACAGGCTGAA GATGAAAGAG AATCTACTGA TGATGAGAGC 
AATCCTCTGG GAAGAGCTGG AATTTGGACA AAGACTCATA CAATATGGTA CAAACCTGTG 
AGGGAGGATG AAGAAAGTAC TAAAGATTGT GTTGGTGGCA AAAGGGGGAG AGCCCAAACA 
GCTCCAACAA AAACTTCCCC CAGAAATGCA AAGAAGCATG ATGAGTTATG GCATGATGGA 
GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATCACCTTTG AAGACCCATC CTTAGATGTA ATACTTCTTT TAAGAGTTTT ACATGCCATC 
AGTCGATATT GGTATTACTT GTATGATAAT GCGATGTGCA AGGAGATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAATT AACAGCAAAA GCAAATAGGC AGCTTCAAGA CCCTCTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTC ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGACA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGGGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGACAGCAGA 
GTTGCGCCTA GATTGGATAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT ACTGAAACAA 
GCTGAGTCTG TGATGCAGGA CCTTGGCAGC TCTCGGGCCA TGTTAGAAAT CCAATATGAA 
AATGAGGTTG GTACAGGTCT GGGGCCAACA CTGGAGTTTT ATGCACTTGT TTCTCAGGAA 
CTACAGAGAG CTGACTTGTG TCTCTGGAGG GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCACATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CTAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCTCT TGGCCTGCCC 
TTTTATAAGT GGATGCTGCG GCAGGAAACT TCATTGACAT CTCATGATTT ATTTGACATT 
GATCCTGTTG TAGCCAGATC AGTATATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTGGAGCAGG ATAAATCCCA GACCAAAGAG AGTCTACAAT ATGCATTGGA AACTCTTACG 
ATGAATGGCT GTTCAGTGGA AGATCTGGGA TTGGATTTTA CGCTGCCTGG GTTTCCCAAC 
ATTGAATTGA AGAAAGGAGG GAAGGATATT CCAGTTACTA TCCACAATTT GGAAGAGTAC 
CTGAGATTGG TGATATTCTG GGCACTAAAT GAAGGTGTTT GTCGACAGTT TGACTCATTC 
AGAGATGGAT TTGAATCGGT CTTCCCGCTC TGCCATCTTC AGTACTTCTA CCCAGAAGAG 
CTGGACCAGC TCCTGTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ATTGATGGAA 
TGCTGCAGGC CTGATCATGG CTATACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTGTTTC TCCAGTTTGT GACTGGTAGC 
CCACGATTGC CAGTTGGAGG ATTTCGGAGT TTAAATCCAC CTCTGACAAT TGTGCGGAAG 
ACATTTGAAT CAACAGAAAA CCCAGATGAC TTCTTACCAT CAGTAATGAC TTGTGTGAAC 
TATCTTAAAT TGCCGGACTA CTCGAGCATT GACATAATGC GGGACAAACT CTTGATAGCA 
GCAAGAGAAG GCCAACAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238663.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236965.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X13, mRNA.

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CloneID	OMu67887
Clone ID Related Accession (Same CDS sequence)	XM_011238663.3 , XM_011238663.2
Accession Version	XM_011238663.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236965.1
CDS	363..6572
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238663.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238663.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238664.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236966.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X16, mRNA.

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CloneID	OMu67888
Clone ID Related Accession (Same CDS sequence)	XM_011238664.3 , XM_011238664.2
Accession Version	XM_011238664.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238664.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236966.1
CDS	363..6563
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238664.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238664.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238665.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236967.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X19, mRNA.

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CloneID	OMu67889
Clone ID Related Accession (Same CDS sequence)	XM_011238665.2 , XM_011238665.3
Accession Version	XM_011238665.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238665.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTACA AATCAATGAA 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAATC CCTTAGCCAA TTCTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTAATGAA AGAAGATCCG 
GAACTGGCTA AGTCTTTCAT TAAGACCTTG TTTGGTGTTC TCTATGAAGT GTACAGCTCC 
TCAGCAGGCC CTGCGGTCAG ACACAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTT 
GCTGATGCTG AACTTCTGAA GGATGTTCTG AAAAATCATG CTGTTTCAAG TCACATTGCT 
TCAATGCTAT CCAGCCAAGA CCTGAAGATA GTAGTTGGAG CTCTTCAGAT GGCAGAAATC 
CTAATGCAAA AGTTACCTGA TATTTTTAGT GTTTACTTCA GGAGAGAAGG TGTGATGCAT 
CAAGTCAAAC ATTTAGCAGA ATCAGAGTCT TTGTTGACAA GTCCCCCCAA GGCATGTACA 
AATGGGTCAG GATCCCTGGG ATCTACAACA CCAGCCAGCA GTGGGACTGC AACGGCAGCA 
ACCAACGCCT CTGCTGATCT GGGGTCACCT AGCTTGCAGC ATAGCAGAGA TGATTCTTTG 
GATCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTCAAGA GAAAACGACT GCCAAAACGA 
GGGCCAAGAA GGCCAAAATA TTCCCCTCCA AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CCACTACTCA GTCACCTAAA TCTTCCTTCC TGGCAAGCTT GAATCCAAAA 
ACATGGGGAA GGTTAAGCGC ACAGTCCAAC AGCAACAATA TCGAACCAGC ACGAACTGCA 
GGAGTTAGTG GTCTTGCCAG GGCAGCCTCA AAAGACACCA TATCCAATAA TAGAGAAAAA 
ATTAAAGGCT GGATTAAGGA GCAGGCACAT AAATTTGTTG AGCGTTATTT TAGTTCTGAG 
AATATGGATG GAAGCAACCC TGCCTTGAAT GTCCTTCAGA GACTTTGTGC TGCAACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCAG ATGTTTCTTC ATTTGAAATC CAACATAGTG GGTTTGTGAA ACAACTGTTG 
CTGTATTTGA CATCTAAGAA TGAAAAGGAT GCTGTCGGCA GAGAGATCCG GTTAAAACGT 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCTGTTGG AAGAGTAGAA 
CCAGTGGGCC ATGCACCTTT GCTGGCATTG GTTCACAAGA TGAACAACTG TCTCAGCCAA 
ATGGAGCAAT TTCCAGTCAA AGTACATGAT TTCCCTAGTG GGAACGGAGC TGGAGGAAGC 
TTTTCTCTCA ACAGAGGATC ACAAGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGT 
CAGTTACAAA GACACCCAGA CTGTGCAAAT GTGAAGCAGT GGAAAGGCGG GCCTGTCAAG 
ATTGATCCCC TGGCTCTGGT GCAAGCTATT GAGAGATACC TTGTGGTTAG AGGTATTTTC 
CGATATGGAA GAGTAAGAGA AGATGATGAA GATAGTGATG ATGATGGGTC AGATGAAGAA 
ATAGATGAGT CTTTGGCTGC TCAATTCTTA AATTCGGGAA ATGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT ACTACCATAT AACATGACCG TGTATCAGGC AGTGCGGCAA 
TTCAGTGTAC AGGCTGAAGA TGAAAGAGAA TCTACTGATG ATGAGAGCAA TCCTCTGGGA 
AGAGCTGGAA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG GGAGGATGAA 
GAAAGTACTA AAGATTGTGT TGGTGGCAAA AGGGGGAGAG CCCAAACAGC TCCAACAAAA 
ACTTCCCCCA GAAATGCAAA GAAGCATGAT GAGTTATGGC ATGATGGAGT GTGCCCGTCA 
GTAGCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT CACCTTTGAA 
GACCCATCCT TAGATGTAAT ACTTCTTTTA AGAGTTTTAC ATGCCATCAG TCGATATTGG 
TATTACTTGT ATGATAATGC GATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAATTAA CAGCAAAAGC AAATAGGCAG CTTCAAGACC CTCTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTCAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGACAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGGGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ACAGCAGAGT TGCGCCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTAC TGAAACAAGC TGAGTCTGTG 
ATGCAGGACC TTGGCAGCTC TCGGGCCATG TTAGAAATCC AATATGAAAA TGAGGTTGGT 
ACAGGTCTGG GGCCAACACT GGAGTTTTAT GCACTTGTTT CTCAGGAACT ACAGAGAGCT 
GACTTGTGTC TCTGGAGGGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ACATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCT 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCTCTTG GCCTGCCCTT TTATAAGTGG 
ATGCTGCGGC AGGAAACTTC ATTGACATCT CATGATTTAT TTGACATTGA TCCTGTTGTA 
GCCAGATCAG TATATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT GGAGCAGGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTGGAAA CTCTTACGAT GAATGGCTGT 
TCAGTGGAAG ATCTGGGATT GGATTTTACG CTGCCTGGGT TTCCCAACAT TGAATTGAAG 
AAAGGAGGGA AGGATATTCC AGTTACTATC CACAATTTGG AAGAGTACCT GAGATTGGTG 
ATATTCTGGG CACTAAATGA AGGTGTTTGT CGACAGTTTG ACTCATTCAG AGATGGATTT 
GAATCGGTCT TCCCGCTCTG CCATCTTCAG TACTTCTACC CAGAAGAGCT GGACCAGCTC 
CTGTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAT TGATGGAATG CTGCAGGCCT 
GATCATGGCT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CTGGTAGCCC ACGATTGCCA 
GTTGGAGGAT TTCGGAGTTT AAATCCACCT CTGACAATTG TGCGGAAGAC ATTTGAATCA 
ACAGAAAACC CAGATGACTT CTTACCATCA GTAATGACTT GTGTGAACTA TCTTAAATTG 
CCGGACTACT CGAGCATTGA CATAATGCGG GACAAACTCT TGATAGCAGC AAGAGAAGGC 
CAACAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236967.1
CDS	363..6506
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238665.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238665.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTACA AATCAATGAA 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAATC CCTTAGCCAA TTCTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTAATGAA AGAAGATCCG 
GAACTGGCTA AGTCTTTCAT TAAGACCTTG TTTGGTGTTC TCTATGAAGT GTACAGCTCC 
TCAGCAGGCC CTGCGGTCAG ACACAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTT 
GCTGATGCTG AACTTCTGAA GGATGTTCTG AAAAATCATG CTGTTTCAAG TCACATTGCT 
TCAATGCTAT CCAGCCAAGA CCTGAAGATA GTAGTTGGAG CTCTTCAGAT GGCAGAAATC 
CTAATGCAAA AGTTACCTGA TATTTTTAGT GTTTACTTCA GGAGAGAAGG TGTGATGCAT 
CAAGTCAAAC ATTTAGCAGA ATCAGAGTCT TTGTTGACAA GTCCCCCCAA GGCATGTACA 
AATGGGTCAG GATCCCTGGG ATCTACAACA CCAGCCAGCA GTGGGACTGC AACGGCAGCA 
ACCAACGCCT CTGCTGATCT GGGGTCACCT AGCTTGCAGC ATAGCAGAGA TGATTCTTTG 
GATCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTCAAGA GAAAACGACT GCCAAAACGA 
GGGCCAAGAA GGCCAAAATA TTCCCCTCCA AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CCACTACTCA GTCACCTAAA TCTTCCTTCC TGGCAAGCTT GAATCCAAAA 
ACATGGGGAA GGTTAAGCGC ACAGTCCAAC AGCAACAATA TCGAACCAGC ACGAACTGCA 
GGAGTTAGTG GTCTTGCCAG GGCAGCCTCA AAAGACACCA TATCCAATAA TAGAGAAAAA 
ATTAAAGGCT GGATTAAGGA GCAGGCACAT AAATTTGTTG AGCGTTATTT TAGTTCTGAG 
AATATGGATG GAAGCAACCC TGCCTTGAAT GTCCTTCAGA GACTTTGTGC TGCAACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCAG ATGTTTCTTC ATTTGAAATC CAACATAGTG GGTTTGTGAA ACAACTGTTG 
CTGTATTTGA CATCTAAGAA TGAAAAGGAT GCTGTCGGCA GAGAGATCCG GTTAAAACGT 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCTGTTGG AAGAGTAGAA 
CCAGTGGGCC ATGCACCTTT GCTGGCATTG GTTCACAAGA TGAACAACTG TCTCAGCCAA 
ATGGAGCAAT TTCCAGTCAA AGTACATGAT TTCCCTAGTG GGAACGGAGC TGGAGGAAGC 
TTTTCTCTCA ACAGAGGATC ACAAGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGT 
CAGTTACAAA GACACCCAGA CTGTGCAAAT GTGAAGCAGT GGAAAGGCGG GCCTGTCAAG 
ATTGATCCCC TGGCTCTGGT GCAAGCTATT GAGAGATACC TTGTGGTTAG AGGTATTTTC 
CGATATGGAA GAGTAAGAGA AGATGATGAA GATAGTGATG ATGATGGGTC AGATGAAGAA 
ATAGATGAGT CTTTGGCTGC TCAATTCTTA AATTCGGGAA ATGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT ACTACCATAT AACATGACCG TGTATCAGGC AGTGCGGCAA 
TTCAGTGTAC AGGCTGAAGA TGAAAGAGAA TCTACTGATG ATGAGAGCAA TCCTCTGGGA 
AGAGCTGGAA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG GGAGGATGAA 
GAAAGTACTA AAGATTGTGT TGGTGGCAAA AGGGGGAGAG CCCAAACAGC TCCAACAAAA 
ACTTCCCCCA GAAATGCAAA GAAGCATGAT GAGTTATGGC ATGATGGAGT GTGCCCGTCA 
GTAGCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT CACCTTTGAA 
GACCCATCCT TAGATGTAAT ACTTCTTTTA AGAGTTTTAC ATGCCATCAG TCGATATTGG 
TATTACTTGT ATGATAATGC GATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAATTAA CAGCAAAAGC AAATAGGCAG CTTCAAGACC CTCTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTCAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGACAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGGGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ACAGCAGAGT TGCGCCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTAC TGAAACAAGC TGAGTCTGTG 
ATGCAGGACC TTGGCAGCTC TCGGGCCATG TTAGAAATCC AATATGAAAA TGAGGTTGGT 
ACAGGTCTGG GGCCAACACT GGAGTTTTAT GCACTTGTTT CTCAGGAACT ACAGAGAGCT 
GACTTGTGTC TCTGGAGGGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ACATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCT 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCTCTTG GCCTGCCCTT TTATAAGTGG 
ATGCTGCGGC AGGAAACTTC ATTGACATCT CATGATTTAT TTGACATTGA TCCTGTTGTA 
GCCAGATCAG TATATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT GGAGCAGGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTGGAAA CTCTTACGAT GAATGGCTGT 
TCAGTGGAAG ATCTGGGATT GGATTTTACG CTGCCTGGGT TTCCCAACAT TGAATTGAAG 
AAAGGAGGGA AGGATATTCC AGTTACTATC CACAATTTGG AAGAGTACCT GAGATTGGTG 
ATATTCTGGG CACTAAATGA AGGTGTTTGT CGACAGTTTG ACTCATTCAG AGATGGATTT 
GAATCGGTCT TCCCGCTCTG CCATCTTCAG TACTTCTACC CAGAAGAGCT GGACCAGCTC 
CTGTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAT TGATGGAATG CTGCAGGCCT 
GATCATGGCT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CTGGTAGCCC ACGATTGCCA 
GTTGGAGGAT TTCGGAGTTT AAATCCACCT CTGACAATTG TGCGGAAGAC ATTTGAATCA 
ACAGAAAACC CAGATGACTT CTTACCATCA GTAATGACTT GTGTGAACTA TCTTAAATTG 
CCGGACTACT CGAGCATTGA CATAATGCGG GACAAACTCT TGATAGCAGC AAGAGAAGGC 
CAACAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238666.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236968.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X20, mRNA.

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CloneID	OMu67890
Clone ID Related Accession (Same CDS sequence)	XM_011238666.2 , XM_011238666.3
Accession Version	XM_011238666.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGCAAAT CAATGAAGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAATCCCT TAGCCAATTC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGATCCGGAA 
CTGGCTAAGT CTTTCATTAA GACCTTGTTT GGTGTTCTCT ATGAAGTGTA CAGCTCCTCA 
GCAGGCCCTG CGGTCAGACA CAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTTGCT 
GATGCTGAAC TTCTGAAGGA TGTTCTGAAA AATCATGCTG TTTCAAGTCA CATTGCTTCA 
ATGCTATCCA GCCAAGACCT GAAGATAGTA GTTGGAGCTC TTCAGATGGC AGAAATCCTA 
ATGCAAAAGT TACCTGATAT TTTTAGTGTT TACTTCAGGA GAGAAGGTGT GATGCATCAA 
GTCAAACATT TAGCAGAATC AGAGTCTTTG TTGACAAGTC CCCCCAAGGC ATGTACAAAT 
GGGTCAGGAT CCCTGGGATC TACAACACCA GCCAGCAGTG GGACTGCAAC GGCAGCAACC 
AACGCCTCTG CTGATCTGGG GTCACCTAGC TTGCAGCATA GCAGAGATGA TTCTTTGGAT 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTCAAGAGAA AACGACTGCC AAAACGAGGG 
CCAAGAAGGC CAAAATATTC CCCTCCAAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAATCT TCCTTCCTGG CAAGCTTGAA TCCAAAAACA 
TGGGGAAGGT TAAGCGCACA GTCCAACAGC AACAATATCG AACCAGCACG AACTGCAGGA 
GTTAGTGGTC TTGCCAGGGC AGCCTCAAAA GACACCATAT CCAATAATAG AGAAAAAATT 
AAAGGCTGGA TTAAGGAGCA GGCACATAAA TTTGTTGAGC GTTATTTTAG TTCTGAGAAT 
ATGGATGGAA GCAACCCTGC CTTGAATGTC CTTCAGAGAC TTTGTGCTGC AACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCAGATG TTTCTTCATT TGAAATCCAA CATAGTGGGT TTGTGAAACA ACTGTTGCTG 
TATTTGACAT CTAAGAATGA AAAGGATGCT GTCGGCAGAG AGATCCGGTT AAAACGTTTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CTGTTGGAAG AGTAGAACCA 
GTGGGCCATG CACCTTTGCT GGCATTGGTT CACAAGATGA ACAACTGTCT CAGCCAAATG 
GAGCAATTTC CAGTCAAAGT ACATGATTTC CCTAGTGGGA ACGGAGCTGG AGGAAGCTTT 
TCTCTCAACA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG TATTTTCCGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236968.1
CDS	363..6503
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238666.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238666.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGCAAAT CAATGAAGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAATCCCT TAGCCAATTC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGATCCGGAA 
CTGGCTAAGT CTTTCATTAA GACCTTGTTT GGTGTTCTCT ATGAAGTGTA CAGCTCCTCA 
GCAGGCCCTG CGGTCAGACA CAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTTGCT 
GATGCTGAAC TTCTGAAGGA TGTTCTGAAA AATCATGCTG TTTCAAGTCA CATTGCTTCA 
ATGCTATCCA GCCAAGACCT GAAGATAGTA GTTGGAGCTC TTCAGATGGC AGAAATCCTA 
ATGCAAAAGT TACCTGATAT TTTTAGTGTT TACTTCAGGA GAGAAGGTGT GATGCATCAA 
GTCAAACATT TAGCAGAATC AGAGTCTTTG TTGACAAGTC CCCCCAAGGC ATGTACAAAT 
GGGTCAGGAT CCCTGGGATC TACAACACCA GCCAGCAGTG GGACTGCAAC GGCAGCAACC 
AACGCCTCTG CTGATCTGGG GTCACCTAGC TTGCAGCATA GCAGAGATGA TTCTTTGGAT 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTCAAGAGAA AACGACTGCC AAAACGAGGG 
CCAAGAAGGC CAAAATATTC CCCTCCAAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAATCT TCCTTCCTGG CAAGCTTGAA TCCAAAAACA 
TGGGGAAGGT TAAGCGCACA GTCCAACAGC AACAATATCG AACCAGCACG AACTGCAGGA 
GTTAGTGGTC TTGCCAGGGC AGCCTCAAAA GACACCATAT CCAATAATAG AGAAAAAATT 
AAAGGCTGGA TTAAGGAGCA GGCACATAAA TTTGTTGAGC GTTATTTTAG TTCTGAGAAT 
ATGGATGGAA GCAACCCTGC CTTGAATGTC CTTCAGAGAC TTTGTGCTGC AACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCAGATG TTTCTTCATT TGAAATCCAA CATAGTGGGT TTGTGAAACA ACTGTTGCTG 
TATTTGACAT CTAAGAATGA AAAGGATGCT GTCGGCAGAG AGATCCGGTT AAAACGTTTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CTGTTGGAAG AGTAGAACCA 
GTGGGCCATG CACCTTTGCT GGCATTGGTT CACAAGATGA ACAACTGTCT CAGCCAAATG 
GAGCAATTTC CAGTCAAAGT ACATGATTTC CCTAGTGGGA ACGGAGCTGG AGGAAGCTTT 
TCTCTCAACA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG TATTTTCCGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238667.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236969.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X22, mRNA.

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CloneID	OMu67891
Clone ID Related Accession (Same CDS sequence)	XM_011238667.3 , XM_011238667.2
Accession Version	XM_011238667.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA GTCCACCAAG 
AAACGCAGTG AGTCCCCACC TGCTGAGCTC CCCAGTTTGA GGAGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA GAGAGGAGCA 
GCTGAGGCCC GGCGGCAAGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GACAGTAAAT 
TCCTCAGCTG CCCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCGG ATGATTCTGA GATGGGACGA 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCTCCTCACC TATTTGGTCC TCTCGGGCCT 
CGGATGTCAC AACTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTTCAAG GACTACAAGC CAGTGATGAA AGTCAACAGC TTCAGGCCGT CATTGAAATG 
TGTCAGTTAT TGGTCATGGG GAATGAGGAG ACACTAGGAG GGTTTCCTGT GAAGAGTGTT 
GTTCCAGCTT TGATAACATT ACTGCAGATG GAACATAATT TTGACATTAT GAATCATGCT 
TGCCGAGCCT TAACATATAT GATGGAAGCT CTCCCCCGGT CATCTGCTGT TGTAGTGGAT 
GCTATCCCTG TTTTCTTAGA AAAGTTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG CGACACAGTA AAGCCATTTT ACAAGCGGGT 
GGTTTGGCAG ATTGCTTGTT GTATCTAGAA TTCTTCAGCA TCAATGCCCA AAGAAATGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACACCAG ATGAGTTTCA TTTTGTGGCA 
GATTCCCTAC CATTGCTTAC CCAAAGGCTA ACTCATCAGG ACAAAAAGTC AGTAGAAAGT 
ACTTGCCTTT GTTTTGCACG TTTAGTGGAC AACTTTCAAC ATGAGGAGAA CTTACTGCAA 
CAGGTTGCTT CCAAAGATCT ACTTACAAAT GTTCAACAAT TGTTGGTAGT GACTCCACCG 
ATTTTAAGTT CTGGGATGTT TATTATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACAT TAGCTGTTCA ACTTATGAAA CAAAGCTTTA CAGACATTGC AGAAACACTT 
CACTTCCTGC TATGTGGTGC CTCCAATGGA AGTTGTCAAG AGCAGATTGA CCTTGTTCCA 
CGAAGTCCTC AAGAGCTGTA TGAGCTGACA TCTTTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAAGAAG GCATCTTCGC AGTTGATACC ATGTTGAAAA AGGGAAATGC ACAGAACACA 
GATGGTGCAA TATGGCAGTG GCGGGATGAC CGAGGCCTCT GGCATCCCTA TAACAGGATT 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAAGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AATCCCTTAG CCAATTCTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTAA TGAAAGAAGA TCCGGAACTG 
GCTAAGTCTT TCATTAAGAC CTTGTTTGGT GTTCTCTATG AAGTGTACAG CTCCTCAGCA 
GGCCCTGCGG TCAGACACAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTTGCTGAT 
GCTGAACTTC TGAAGGATGT TCTGAAAAAT CATGCTGTTT CAAGTCACAT TGCTTCAATG 
CTATCCAGCC AAGACCTGAA GATAGTAGTT GGAGCTCTTC AGATGGCAGA AATCCTAATG 
CAAAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGGAGAG AAGGTGTGAT GCATCAAGTC 
AAACATTTAG CAGAATCAGA GTCTTTGTTG ACAAGTCCCC CCAAGGCATG TACAAATGGG 
TCAGGATCCC TGGGATCTAC AACACCAGCC AGCAGTGGGA CTGCAACGGC AGCAACCAAC 
GCCTCTGCTG ATCTGGGGTC ACCTAGCTTG CAGCATAGCA GAGATGATTC TTTGGATCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTC AAGAGAAAAC GACTGCCAAA ACGAGGGCCA 
AGAAGGCCAA AATATTCCCC TCCAAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAATCTTCC TTCCTGGCAA GCTTGAATCC AAAAACATGG 
GGAAGGTTAA GCGCACAGTC CAACAGCAAC AATATCGAAC CAGCACGAAC TGCAGGAGTT 
AGTGGTCTTG CCAGGGCAGC CTCAAAAGAC ACCATATCCA ATAATAGAGA AAAAATTAAA 
GGCTGGATTA AGGAGCAGGC ACATAAATTT GTTGAGCGTT ATTTTAGTTC TGAGAATATG 
GATGGAAGCA ACCCTGCCTT GAATGTCCTT CAGAGACTTT GTGCTGCAAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCAGATGTTT CTTCATTTGA AATCCAACAT AGTGGGTTTG TGAAACAACT GTTGCTGTAT 
TTGACATCTA AGAATGAAAA GGATGCTGTC GGCAGAGAGA TCCGGTTAAA ACGTTTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCTG TTGGAAGAGT AGAACCAGTG 
GGCCATGCAC CTTTGCTGGC ATTGGTTCAC AAGATGAACA ACTGTCTCAG CCAAATGGAG 
CAATTTCCAG TCAAAGTACA TGATTTCCCT AGTGGGAACG GAGCTGGAGG AAGCTTTTCT 
CTCAACAGAG GATCACAAGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGTCAGTTA 
CAAAGACACC CAGACTGTGC AAATGTGAAG CAGTGGAAAG GCGGGCCTGT CAAGATTGAT 
CCCCTGGCTC TGGTGCAAGC TATTGAGAGA TACCTTGTGG TTAGAGGTAT TTTCCGATAT 
GGAAGAGTAA GAGAAGATGA TGAAGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAGTCTTTGG CTGCTCAATT CTTAAATTCG GGAAATGTAA GGCACAGGCT ACAGTTTTAT 
ATTGGAGAAC ATTTACTACC ATATAACATG ACCGTGTATC AGGCAGTGCG GCAATTCAGT 
GTACAGGCTG AAGATGAAAG AGAATCTACT GATGATGAGA GCAATCCTCT GGGAAGAGCT 
GGAATTTGGA CAAAGACTCA TACAATATGG TACAAACCTG TGAGGGAGGA TGAAGAAAGT 
ACTAAAGATT GTGTTGGTGG CAAAAGGGGG AGAGCCCAAA CAGCTCCAAC AAAAACTTCC 
CCCAGAAATG CAAAGAAGCA TGATGAGTTA TGGCATGATG GAGTGTGCCC GTCAGTAGCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATCACCTT TGAAGACCCA 
TCCTTAGATG TAATACTTCT TTTAAGAGTT TTACATGCCA TCAGTCGATA TTGGTATTAC 
TTGTATGATA ATGCGATGTG CAAGGAGATT ATTCCAACTA GTGAATTTAT TAACAGTAAA 
TTAACAGCAA AAGCAAATAG GCAGCTTCAA GACCCTCTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TCACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGA 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGGG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGACAGCA GAGTTGCGCC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTACTGAAAC AAGCTGAGTC TGTGATGCAG 
GACCTTGGCA GCTCTCGGGC CATGTTAGAA ATCCAATATG AAAATGAGGT TGGTACAGGT 
CTGGGGCCAA CACTGGAGTT TTATGCACTT GTTTCTCAGG AACTACAGAG AGCTGACTTG 
TGTCTCTGGA GGGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCACATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCTAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCT CTTGGCCTGC CCTTTTATAA GTGGATGCTG 
CGGCAGGAAA CTTCATTGAC ATCTCATGAT TTATTTGACA TTGATCCTGT TGTAGCCAGA 
TCAGTATATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTGGAGCA GGATAAATCC 
CAGACCAAAG AGAGTCTACA ATATGCATTG GAAACTCTTA CGATGAATGG CTGTTCAGTG 
GAAGATCTGG GATTGGATTT TACGCTGCCT GGGTTTCCCA ACATTGAATT GAAGAAAGGA 
GGGAAGGATA TTCCAGTTAC TATCCACAAT TTGGAAGAGT ACCTGAGATT GGTGATATTC 
TGGGCACTAA ATGAAGGTGT TTGTCGACAG TTTGACTCAT TCAGAGATGG ATTTGAATCG 
GTCTTCCCGC TCTGCCATCT TCAGTACTTC TACCCAGAAG AGCTGGACCA GCTCCTGTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACATTGATGG AATGCTGCAG GCCTGATCAT 
GGCTATACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTGTT TCTCCAGTTT GTGACTGGTA GCCCACGATT GCCAGTTGGA 
GGATTTCGGA GTTTAAATCC ACCTCTGACA ATTGTGCGGA AGACATTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTACC ATCAGTAATG ACTTGTGTGA ACTATCTTAA ATTGCCGGAC 
TACTCGAGCA TTGACATAAT GCGGGACAAA CTCTTGATAG CAGCAAGAGA AGGCCAACAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236969.1
CDS	363..6500
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238667.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238667.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA GTCCACCAAG 
AAACGCAGTG AGTCCCCACC TGCTGAGCTC CCCAGTTTGA GGAGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA GAGAGGAGCA 
GCTGAGGCCC GGCGGCAAGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GACAGTAAAT 
TCCTCAGCTG CCCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCGG ATGATTCTGA GATGGGACGA 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCTCCTCACC TATTTGGTCC TCTCGGGCCT 
CGGATGTCAC AACTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTTCAAG GACTACAAGC CAGTGATGAA AGTCAACAGC TTCAGGCCGT CATTGAAATG 
TGTCAGTTAT TGGTCATGGG GAATGAGGAG ACACTAGGAG GGTTTCCTGT GAAGAGTGTT 
GTTCCAGCTT TGATAACATT ACTGCAGATG GAACATAATT TTGACATTAT GAATCATGCT 
TGCCGAGCCT TAACATATAT GATGGAAGCT CTCCCCCGGT CATCTGCTGT TGTAGTGGAT 
GCTATCCCTG TTTTCTTAGA AAAGTTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG CGACACAGTA AAGCCATTTT ACAAGCGGGT 
GGTTTGGCAG ATTGCTTGTT GTATCTAGAA TTCTTCAGCA TCAATGCCCA AAGAAATGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACACCAG ATGAGTTTCA TTTTGTGGCA 
GATTCCCTAC CATTGCTTAC CCAAAGGCTA ACTCATCAGG ACAAAAAGTC AGTAGAAAGT 
ACTTGCCTTT GTTTTGCACG TTTAGTGGAC AACTTTCAAC ATGAGGAGAA CTTACTGCAA 
CAGGTTGCTT CCAAAGATCT ACTTACAAAT GTTCAACAAT TGTTGGTAGT GACTCCACCG 
ATTTTAAGTT CTGGGATGTT TATTATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACAT TAGCTGTTCA ACTTATGAAA CAAAGCTTTA CAGACATTGC AGAAACACTT 
CACTTCCTGC TATGTGGTGC CTCCAATGGA AGTTGTCAAG AGCAGATTGA CCTTGTTCCA 
CGAAGTCCTC AAGAGCTGTA TGAGCTGACA TCTTTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAAGAAG GCATCTTCGC AGTTGATACC ATGTTGAAAA AGGGAAATGC ACAGAACACA 
GATGGTGCAA TATGGCAGTG GCGGGATGAC CGAGGCCTCT GGCATCCCTA TAACAGGATT 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAAGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AATCCCTTAG CCAATTCTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTAA TGAAAGAAGA TCCGGAACTG 
GCTAAGTCTT TCATTAAGAC CTTGTTTGGT GTTCTCTATG AAGTGTACAG CTCCTCAGCA 
GGCCCTGCGG TCAGACACAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTTGCTGAT 
GCTGAACTTC TGAAGGATGT TCTGAAAAAT CATGCTGTTT CAAGTCACAT TGCTTCAATG 
CTATCCAGCC AAGACCTGAA GATAGTAGTT GGAGCTCTTC AGATGGCAGA AATCCTAATG 
CAAAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGGAGAG AAGGTGTGAT GCATCAAGTC 
AAACATTTAG CAGAATCAGA GTCTTTGTTG ACAAGTCCCC CCAAGGCATG TACAAATGGG 
TCAGGATCCC TGGGATCTAC AACACCAGCC AGCAGTGGGA CTGCAACGGC AGCAACCAAC 
GCCTCTGCTG ATCTGGGGTC ACCTAGCTTG CAGCATAGCA GAGATGATTC TTTGGATCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTC AAGAGAAAAC GACTGCCAAA ACGAGGGCCA 
AGAAGGCCAA AATATTCCCC TCCAAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAATCTTCC TTCCTGGCAA GCTTGAATCC AAAAACATGG 
GGAAGGTTAA GCGCACAGTC CAACAGCAAC AATATCGAAC CAGCACGAAC TGCAGGAGTT 
AGTGGTCTTG CCAGGGCAGC CTCAAAAGAC ACCATATCCA ATAATAGAGA AAAAATTAAA 
GGCTGGATTA AGGAGCAGGC ACATAAATTT GTTGAGCGTT ATTTTAGTTC TGAGAATATG 
GATGGAAGCA ACCCTGCCTT GAATGTCCTT CAGAGACTTT GTGCTGCAAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCAGATGTTT CTTCATTTGA AATCCAACAT AGTGGGTTTG TGAAACAACT GTTGCTGTAT 
TTGACATCTA AGAATGAAAA GGATGCTGTC GGCAGAGAGA TCCGGTTAAA ACGTTTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCTG TTGGAAGAGT AGAACCAGTG 
GGCCATGCAC CTTTGCTGGC ATTGGTTCAC AAGATGAACA ACTGTCTCAG CCAAATGGAG 
CAATTTCCAG TCAAAGTACA TGATTTCCCT AGTGGGAACG GAGCTGGAGG AAGCTTTTCT 
CTCAACAGAG GATCACAAGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGTCAGTTA 
CAAAGACACC CAGACTGTGC AAATGTGAAG CAGTGGAAAG GCGGGCCTGT CAAGATTGAT 
CCCCTGGCTC TGGTGCAAGC TATTGAGAGA TACCTTGTGG TTAGAGGTAT TTTCCGATAT 
GGAAGAGTAA GAGAAGATGA TGAAGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAGTCTTTGG CTGCTCAATT CTTAAATTCG GGAAATGTAA GGCACAGGCT ACAGTTTTAT 
ATTGGAGAAC ATTTACTACC ATATAACATG ACCGTGTATC AGGCAGTGCG GCAATTCAGT 
GTACAGGCTG AAGATGAAAG AGAATCTACT GATGATGAGA GCAATCCTCT GGGAAGAGCT 
GGAATTTGGA CAAAGACTCA TACAATATGG TACAAACCTG TGAGGGAGGA TGAAGAAAGT 
ACTAAAGATT GTGTTGGTGG CAAAAGGGGG AGAGCCCAAA CAGCTCCAAC AAAAACTTCC 
CCCAGAAATG CAAAGAAGCA TGATGAGTTA TGGCATGATG GAGTGTGCCC GTCAGTAGCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATCACCTT TGAAGACCCA 
TCCTTAGATG TAATACTTCT TTTAAGAGTT TTACATGCCA TCAGTCGATA TTGGTATTAC 
TTGTATGATA ATGCGATGTG CAAGGAGATT ATTCCAACTA GTGAATTTAT TAACAGTAAA 
TTAACAGCAA AAGCAAATAG GCAGCTTCAA GACCCTCTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TCACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGA 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGGG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGACAGCA GAGTTGCGCC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTACTGAAAC AAGCTGAGTC TGTGATGCAG 
GACCTTGGCA GCTCTCGGGC CATGTTAGAA ATCCAATATG AAAATGAGGT TGGTACAGGT 
CTGGGGCCAA CACTGGAGTT TTATGCACTT GTTTCTCAGG AACTACAGAG AGCTGACTTG 
TGTCTCTGGA GGGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCACATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCTAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCT CTTGGCCTGC CCTTTTATAA GTGGATGCTG 
CGGCAGGAAA CTTCATTGAC ATCTCATGAT TTATTTGACA TTGATCCTGT TGTAGCCAGA 
TCAGTATATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTGGAGCA GGATAAATCC 
CAGACCAAAG AGAGTCTACA ATATGCATTG GAAACTCTTA CGATGAATGG CTGTTCAGTG 
GAAGATCTGG GATTGGATTT TACGCTGCCT GGGTTTCCCA ACATTGAATT GAAGAAAGGA 
GGGAAGGATA TTCCAGTTAC TATCCACAAT TTGGAAGAGT ACCTGAGATT GGTGATATTC 
TGGGCACTAA ATGAAGGTGT TTGTCGACAG TTTGACTCAT TCAGAGATGG ATTTGAATCG 
GTCTTCCCGC TCTGCCATCT TCAGTACTTC TACCCAGAAG AGCTGGACCA GCTCCTGTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACATTGATGG AATGCTGCAG GCCTGATCAT 
GGCTATACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTGTT TCTCCAGTTT GTGACTGGTA GCCCACGATT GCCAGTTGGA 
GGATTTCGGA GTTTAAATCC ACCTCTGACA ATTGTGCGGA AGACATTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTACC ATCAGTAATG ACTTGTGTGA ACTATCTTAA ATTGCCGGAC 
TACTCGAGCA TTGACATAAT GCGGGACAAA CTCTTGATAG CAGCAAGAGA AGGCCAACAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238669.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236971.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X29, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_011238669.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236971.1
CDS	132..6230
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238669.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238669.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238670.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236972.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X30, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_011238670.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236972.1
CDS	256..6354
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238670.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238670.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238671.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236973.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X31, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_011238671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236973.1
CDS	292..6390
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238671.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238671.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249149.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105009.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X33, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_030249149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105009.1
CDS	140..6238
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249149.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249149.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238672.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236974.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X32, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_011238672.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238672.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236974.1
CDS	134..6232
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238672.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238672.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238668.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236970.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X28, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_011238668.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236970.1
CDS	363..6461
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238668.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238668.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249155.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105015.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X34, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_030249155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105015.1
CDS	185..6283
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249155.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249155.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249195.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105055.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X37, mRNA.

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CloneID	OMu67893
Clone ID Related Accession (Same CDS sequence)	XM_011238673.2 , XM_030249195.1 , XM_011238673.3 , XM_030249168.1 , XM_030249180.1
Accession Version	XM_030249195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAAGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAATCCCTT AGCCAATTCT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT AATGAAAGAA 
GATCCGGAAC TGGCTAAGTC TTTCATTAAG ACCTTGTTTG GTGTTCTCTA TGAAGTGTAC 
AGCTCCTCAG CAGGCCCTGC GGTCAGACAC AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCTG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCAA TGCTATCCAG CCAAGACCTG AAGATAGTAG TTGGAGCTCT TCAGATGGCA 
GAAATCCTAA TGCAAAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGGAG AGAAGGTGTG 
ATGCATCAAG TCAAACATTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC CCCCAAGGCA 
TGTACAAATG GGTCAGGATC CCTGGGATCT ACAACACCAG CCAGCAGTGG GACTGCAACG 
GCAGCAACCA ACGCCTCTGC TGATCTGGGG TCACCTAGCT TGCAGCATAG CAGAGATGAT 
TCTTTGGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TCAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAATATTCC CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CCTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAATATCGA ACCAGCACGA 
ACTGCAGGAG TTAGTGGTCT TGCCAGGGCA GCCTCAAAAG ACACCATATC CAATAATAGA 
GAAAAAATTA AAGGCTGGAT TAAGGAGCAG GCACATAAAT TTGTTGAGCG TTATTTTAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCC TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCTTCATTT GAAATCCAAC ATAGTGGGTT TGTGAAACAA 
CTGTTGCTGT ATTTGACATC TAAGAATGAA AAGGATGCTG TCGGCAGAGA GATCCGGTTA 
AAACGTTTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC TGTTGGAAGA 
GTAGAACCAG TGGGCCATGC ACCTTTGCTG GCATTGGTTC ACAAGATGAA CAACTGTCTC 
AGCCAAATGG AGCAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGGAA CGGAGCTGGA 
GGAAGCTTTT CTCTCAACAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105055.1
CDS	193..6282
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249195.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249195.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAAGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAATCCCTT AGCCAATTCT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT AATGAAAGAA 
GATCCGGAAC TGGCTAAGTC TTTCATTAAG ACCTTGTTTG GTGTTCTCTA TGAAGTGTAC 
AGCTCCTCAG CAGGCCCTGC GGTCAGACAC AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCTG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCAA TGCTATCCAG CCAAGACCTG AAGATAGTAG TTGGAGCTCT TCAGATGGCA 
GAAATCCTAA TGCAAAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGGAG AGAAGGTGTG 
ATGCATCAAG TCAAACATTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC CCCCAAGGCA 
TGTACAAATG GGTCAGGATC CCTGGGATCT ACAACACCAG CCAGCAGTGG GACTGCAACG 
GCAGCAACCA ACGCCTCTGC TGATCTGGGG TCACCTAGCT TGCAGCATAG CAGAGATGAT 
TCTTTGGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TCAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAATATTCC CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CCTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAATATCGA ACCAGCACGA 
ACTGCAGGAG TTAGTGGTCT TGCCAGGGCA GCCTCAAAAG ACACCATATC CAATAATAGA 
GAAAAAATTA AAGGCTGGAT TAAGGAGCAG GCACATAAAT TTGTTGAGCG TTATTTTAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCC TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCTTCATTT GAAATCCAAC ATAGTGGGTT TGTGAAACAA 
CTGTTGCTGT ATTTGACATC TAAGAATGAA AAGGATGCTG TCGGCAGAGA GATCCGGTTA 
AAACGTTTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC TGTTGGAAGA 
GTAGAACCAG TGGGCCATGC ACCTTTGCTG GCATTGGTTC ACAAGATGAA CAACTGTCTC 
AGCCAAATGG AGCAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGGAA CGGAGCTGGA 
GGAAGCTTTT CTCTCAACAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238673.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236975.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X38, mRNA.

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CloneID	OMu67893
Clone ID Related Accession (Same CDS sequence)	XM_011238673.2 , XM_030249195.1 , XM_011238673.3 , XM_030249168.1 , XM_030249180.1
Accession Version	XM_011238673.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAAGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAATCCCTT AGCCAATTCT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT AATGAAAGAA 
GATCCGGAAC TGGCTAAGTC TTTCATTAAG ACCTTGTTTG GTGTTCTCTA TGAAGTGTAC 
AGCTCCTCAG CAGGCCCTGC GGTCAGACAC AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCTG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCAA TGCTATCCAG CCAAGACCTG AAGATAGTAG TTGGAGCTCT TCAGATGGCA 
GAAATCCTAA TGCAAAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGGAG AGAAGGTGTG 
ATGCATCAAG TCAAACATTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC CCCCAAGGCA 
TGTACAAATG GGTCAGGATC CCTGGGATCT ACAACACCAG CCAGCAGTGG GACTGCAACG 
GCAGCAACCA ACGCCTCTGC TGATCTGGGG TCACCTAGCT TGCAGCATAG CAGAGATGAT 
TCTTTGGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TCAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAATATTCC CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CCTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAATATCGA ACCAGCACGA 
ACTGCAGGAG TTAGTGGTCT TGCCAGGGCA GCCTCAAAAG ACACCATATC CAATAATAGA 
GAAAAAATTA AAGGCTGGAT TAAGGAGCAG GCACATAAAT TTGTTGAGCG TTATTTTAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCC TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCTTCATTT GAAATCCAAC ATAGTGGGTT TGTGAAACAA 
CTGTTGCTGT ATTTGACATC TAAGAATGAA AAGGATGCTG TCGGCAGAGA GATCCGGTTA 
AAACGTTTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC TGTTGGAAGA 
GTAGAACCAG TGGGCCATGC ACCTTTGCTG GCATTGGTTC ACAAGATGAA CAACTGTCTC 
AGCCAAATGG AGCAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGGAA CGGAGCTGGA 
GGAAGCTTTT CTCTCAACAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236975.1
CDS	134..6223
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238673.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238673.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAAGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAATCCCTT AGCCAATTCT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT AATGAAAGAA 
GATCCGGAAC TGGCTAAGTC TTTCATTAAG ACCTTGTTTG GTGTTCTCTA TGAAGTGTAC 
AGCTCCTCAG CAGGCCCTGC GGTCAGACAC AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCTG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCAA TGCTATCCAG CCAAGACCTG AAGATAGTAG TTGGAGCTCT TCAGATGGCA 
GAAATCCTAA TGCAAAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGGAG AGAAGGTGTG 
ATGCATCAAG TCAAACATTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC CCCCAAGGCA 
TGTACAAATG GGTCAGGATC CCTGGGATCT ACAACACCAG CCAGCAGTGG GACTGCAACG 
GCAGCAACCA ACGCCTCTGC TGATCTGGGG TCACCTAGCT TGCAGCATAG CAGAGATGAT 
TCTTTGGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TCAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAATATTCC CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CCTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAATATCGA ACCAGCACGA 
ACTGCAGGAG TTAGTGGTCT TGCCAGGGCA GCCTCAAAAG ACACCATATC CAATAATAGA 
GAAAAAATTA AAGGCTGGAT TAAGGAGCAG GCACATAAAT TTGTTGAGCG TTATTTTAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCC TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCTTCATTT GAAATCCAAC ATAGTGGGTT TGTGAAACAA 
CTGTTGCTGT ATTTGACATC TAAGAATGAA AAGGATGCTG TCGGCAGAGA GATCCGGTTA 
AAACGTTTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC TGTTGGAAGA 
GTAGAACCAG TGGGCCATGC ACCTTTGCTG GCATTGGTTC ACAAGATGAA CAACTGTCTC 
AGCCAAATGG AGCAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGGAA CGGAGCTGGA 
GGAAGCTTTT CTCTCAACAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249168.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105028.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X35, mRNA.

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CloneID	OMu67893
Clone ID Related Accession (Same CDS sequence)	XM_011238673.2 , XM_030249195.1 , XM_011238673.3 , XM_030249168.1 , XM_030249180.1
Accession Version	XM_030249168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAAGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAATCCCTT AGCCAATTCT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT AATGAAAGAA 
GATCCGGAAC TGGCTAAGTC TTTCATTAAG ACCTTGTTTG GTGTTCTCTA TGAAGTGTAC 
AGCTCCTCAG CAGGCCCTGC GGTCAGACAC AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCTG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCAA TGCTATCCAG CCAAGACCTG AAGATAGTAG TTGGAGCTCT TCAGATGGCA 
GAAATCCTAA TGCAAAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGGAG AGAAGGTGTG 
ATGCATCAAG TCAAACATTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC CCCCAAGGCA 
TGTACAAATG GGTCAGGATC CCTGGGATCT ACAACACCAG CCAGCAGTGG GACTGCAACG 
GCAGCAACCA ACGCCTCTGC TGATCTGGGG TCACCTAGCT TGCAGCATAG CAGAGATGAT 
TCTTTGGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TCAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAATATTCC CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CCTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAATATCGA ACCAGCACGA 
ACTGCAGGAG TTAGTGGTCT TGCCAGGGCA GCCTCAAAAG ACACCATATC CAATAATAGA 
GAAAAAATTA AAGGCTGGAT TAAGGAGCAG GCACATAAAT TTGTTGAGCG TTATTTTAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCC TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCTTCATTT GAAATCCAAC ATAGTGGGTT TGTGAAACAA 
CTGTTGCTGT ATTTGACATC TAAGAATGAA AAGGATGCTG TCGGCAGAGA GATCCGGTTA 
AAACGTTTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC TGTTGGAAGA 
GTAGAACCAG TGGGCCATGC ACCTTTGCTG GCATTGGTTC ACAAGATGAA CAACTGTCTC 
AGCCAAATGG AGCAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGGAA CGGAGCTGGA 
GGAAGCTTTT CTCTCAACAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105028.1
CDS	363..6452
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249168.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249168.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAAGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAATCCCTT AGCCAATTCT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT AATGAAAGAA 
GATCCGGAAC TGGCTAAGTC TTTCATTAAG ACCTTGTTTG GTGTTCTCTA TGAAGTGTAC 
AGCTCCTCAG CAGGCCCTGC GGTCAGACAC AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCTG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCAA TGCTATCCAG CCAAGACCTG AAGATAGTAG TTGGAGCTCT TCAGATGGCA 
GAAATCCTAA TGCAAAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGGAG AGAAGGTGTG 
ATGCATCAAG TCAAACATTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC CCCCAAGGCA 
TGTACAAATG GGTCAGGATC CCTGGGATCT ACAACACCAG CCAGCAGTGG GACTGCAACG 
GCAGCAACCA ACGCCTCTGC TGATCTGGGG TCACCTAGCT TGCAGCATAG CAGAGATGAT 
TCTTTGGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TCAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAATATTCC CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CCTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAATATCGA ACCAGCACGA 
ACTGCAGGAG TTAGTGGTCT TGCCAGGGCA GCCTCAAAAG ACACCATATC CAATAATAGA 
GAAAAAATTA AAGGCTGGAT TAAGGAGCAG GCACATAAAT TTGTTGAGCG TTATTTTAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCC TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCTTCATTT GAAATCCAAC ATAGTGGGTT TGTGAAACAA 
CTGTTGCTGT ATTTGACATC TAAGAATGAA AAGGATGCTG TCGGCAGAGA GATCCGGTTA 
AAACGTTTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC TGTTGGAAGA 
GTAGAACCAG TGGGCCATGC ACCTTTGCTG GCATTGGTTC ACAAGATGAA CAACTGTCTC 
AGCCAAATGG AGCAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGGAA CGGAGCTGGA 
GGAAGCTTTT CTCTCAACAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249180.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105040.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X36, mRNA.

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CloneID	OMu67893
Clone ID Related Accession (Same CDS sequence)	XM_011238673.2 , XM_030249195.1 , XM_011238673.3 , XM_030249168.1 , XM_030249180.1
Accession Version	XM_030249180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAAGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAATCCCTT AGCCAATTCT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT AATGAAAGAA 
GATCCGGAAC TGGCTAAGTC TTTCATTAAG ACCTTGTTTG GTGTTCTCTA TGAAGTGTAC 
AGCTCCTCAG CAGGCCCTGC GGTCAGACAC AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCTG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCAA TGCTATCCAG CCAAGACCTG AAGATAGTAG TTGGAGCTCT TCAGATGGCA 
GAAATCCTAA TGCAAAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGGAG AGAAGGTGTG 
ATGCATCAAG TCAAACATTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC CCCCAAGGCA 
TGTACAAATG GGTCAGGATC CCTGGGATCT ACAACACCAG CCAGCAGTGG GACTGCAACG 
GCAGCAACCA ACGCCTCTGC TGATCTGGGG TCACCTAGCT TGCAGCATAG CAGAGATGAT 
TCTTTGGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TCAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAATATTCC CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CCTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAATATCGA ACCAGCACGA 
ACTGCAGGAG TTAGTGGTCT TGCCAGGGCA GCCTCAAAAG ACACCATATC CAATAATAGA 
GAAAAAATTA AAGGCTGGAT TAAGGAGCAG GCACATAAAT TTGTTGAGCG TTATTTTAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCC TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCTTCATTT GAAATCCAAC ATAGTGGGTT TGTGAAACAA 
CTGTTGCTGT ATTTGACATC TAAGAATGAA AAGGATGCTG TCGGCAGAGA GATCCGGTTA 
AAACGTTTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC TGTTGGAAGA 
GTAGAACCAG TGGGCCATGC ACCTTTGCTG GCATTGGTTC ACAAGATGAA CAACTGTCTC 
AGCCAAATGG AGCAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGGAA CGGAGCTGGA 
GGAAGCTTTT CTCTCAACAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105040.1
CDS	133..6222
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249180.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249180.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAAGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAATCCCTT AGCCAATTCT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT AATGAAAGAA 
GATCCGGAAC TGGCTAAGTC TTTCATTAAG ACCTTGTTTG GTGTTCTCTA TGAAGTGTAC 
AGCTCCTCAG CAGGCCCTGC GGTCAGACAC AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCTG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCAA TGCTATCCAG CCAAGACCTG AAGATAGTAG TTGGAGCTCT TCAGATGGCA 
GAAATCCTAA TGCAAAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGGAG AGAAGGTGTG 
ATGCATCAAG TCAAACATTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC CCCCAAGGCA 
TGTACAAATG GGTCAGGATC CCTGGGATCT ACAACACCAG CCAGCAGTGG GACTGCAACG 
GCAGCAACCA ACGCCTCTGC TGATCTGGGG TCACCTAGCT TGCAGCATAG CAGAGATGAT 
TCTTTGGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TCAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAATATTCC CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CCTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAATATCGA ACCAGCACGA 
ACTGCAGGAG TTAGTGGTCT TGCCAGGGCA GCCTCAAAAG ACACCATATC CAATAATAGA 
GAAAAAATTA AAGGCTGGAT TAAGGAGCAG GCACATAAAT TTGTTGAGCG TTATTTTAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCC TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCTTCATTT GAAATCCAAC ATAGTGGGTT TGTGAAACAA 
CTGTTGCTGT ATTTGACATC TAAGAATGAA AAGGATGCTG TCGGCAGAGA GATCCGGTTA 
AAACGTTTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC TGTTGGAAGA 
GTAGAACCAG TGGGCCATGC ACCTTTGCTG GCATTGGTTC ACAAGATGAA CAACTGTCTC 
AGCCAAATGG AGCAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGGAA CGGAGCTGGA 
GGAAGCTTTT CTCTCAACAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_017316627.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172116.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X21, mRNA.

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CloneID	OMu76980
Clone ID Related Accession (Same CDS sequence)	XM_017316627.2 , XM_017316627.1
Accession Version	XM_017316627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CTCTGTTAGA GGCCAGGGGT CTTCCTCCTC ACCTATTTGG TCCTCTCGGG 
CCTCGGATGT CACAACTTTT CCATAGAACA ATTGGAAGCG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTTC AAGGACTACA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC CGTCATTGAA 
ATGTGTCAGT TATTGGTCAT GGGGAATGAG GAGACACTAG GAGGGTTTCC TGTGAAGAGT 
GTTGTTCCAG CTTTGATAAC ATTACTGCAG ATGGAACATA ATTTTGACAT TATGAATCAT 
GCTTGCCGAG CCTTAACATA TATGATGGAA GCTCTCCCCC GGTCATCTGC TGTTGTAGTG 
GATGCTATCC CTGTTTTCTT AGAAAAGTTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGCGACACA GTAAAGCCAT TTTACAAGCG 
GGTGGTTTGG CAGATTGCTT GTTGTATCTA GAATTCTTCA GCATCAATGC CCAAAGAAAT 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACAC CAGATGAGTT TCATTTTGTG 
GCAGATTCCC TACCATTGCT TACCCAAAGG CTAACTCATC AGGACAAAAA GTCAGTAGAA 
AGTACTTGCC TTTGTTTTGC ACGTTTAGTG GACAACTTTC AACATGAGGA GAACTTACTG 
CAACAGGTTG CTTCCAAAGA TCTACTTACA AATGTTCAAC AATTGTTGGT AGTGACTCCA 
CCGATTTTAA GTTCTGGGAT GTTTATTATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CATTAGCTGT TCAACTTATG AAACAAAGCT TTACAGACAT TGCAGAAACA 
CTTCACTTCC TGCTATGTGG TGCCTCCAAT GGAAGTTGTC AAGAGCAGAT TGACCTTGTT 
CCACGAAGTC CTCAAGAGCT GTATGAGCTG ACATCTTTGA TTTGTGAACT TATGCCATGT 
TTACCAAAAG AAGGCATCTT CGCAGTTGAT ACCATGTTGA AAAAGGGAAA TGCACAGAAC 
ACAGATGGTG CAATATGGCA GTGGCGGGAT GACCGAGGCC TCTGGCATCC CTATAACAGG 
ATTGACAGCC GGATCATTGA GGTAGCAGCC CATCAGGTCG GTGAGGATGA GATAAGCTTG 
TCCACTCTGG GACGAGTTTA TACTATTGAT TTTAATTCTA TGCAGCAAAT CAATGAAGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAATCCCT TAGCCAATTC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGATCCGGAA 
CTGGCTAAGT CTTTCATTAA GACCTTGTTT GGTGTTCTCT ATGAAGTGTA CAGCTCCTCA 
GCAGGCCCTG CGGTCAGACA CAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTTGCT 
GATGCTGAAC TTCTGAAGGA TGTTCTGAAA AATCATGCTG TTTCAAGTCA CATTGCTTCA 
ATGCTATCCA GCCAAGACCT GAAGATAGTA GTTGGAGCTC TTCAGATGGC AGAAATCCTA 
ATGCAAAAGT TACCTGATAT TTTTAGTGTT TACTTCAGGA GAGAAGGTGT GATGCATCAA 
GTCAAACATT TAGCAGAATC AGAGTCTTTG TTGACAAGTC CCCCCAAGGC ATGTACAAAT 
GGGTCAGGAT CCCTGGGATC TACAACACCA GCCAGCAGTG GGACTGCAAC GGCAGCAACC 
AACGCCTCTG CTGATCTGGG GTCACCTAGC TTGCAGCATA GCAGAGATGA TTCTTTGGAT 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTCAAGAGAA AACGACTGCC AAAACGAGGG 
CCAAGAAGGC CAAAATATTC CCCTCCAAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAATCT TCCTTCCTGG CAAGCTTGAA TCCAAAAACA 
TGGGGAAGGT TAAGCGCACA GTCCAACAGC AACAATATCG AACCAGCACG AACTGCAGGA 
GTTAGTGGTC TTGCCAGGGC AGCCTCAAAA GACACCATAT CCAATAATAG AGAAAAAATT 
AAAGGCTGGA TTAAGGAGCA GGCACATAAA TTTGTTGAGC GTTATTTTAG TTCTGAGAAT 
ATGGATGGAA GCAACCCTGC CTTGAATGTC CTTCAGAGAC TTTGTGCTGC AACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCAGATG TTTCTTCATT TGAAATCCAA CATAGTGGGT TTGTGAAACA ACTGTTGCTG 
TATTTGACAT CTAAGAATGA AAAGGATGCT GTCGGCAGAG AGATCCGGTT AAAACGTTTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CTGTTGGAAG AGTAGAACCA 
GTGGGCCATG CACCTTTGCT GGCATTGGTT CACAAGATGA ACAACTGTCT CAGCCAAATG 
GAGCAATTTC CAGTCAAAGT ACATGATTTC CCTAGTGGGA ACGGAGCTGG AGGAAGCTTT 
TCTCTCAACA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG TATTTTCCGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172116.1
CDS	363..6503
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017316627.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316627.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CTCTGTTAGA GGCCAGGGGT CTTCCTCCTC ACCTATTTGG TCCTCTCGGG 
CCTCGGATGT CACAACTTTT CCATAGAACA ATTGGAAGCG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTTC AAGGACTACA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC CGTCATTGAA 
ATGTGTCAGT TATTGGTCAT GGGGAATGAG GAGACACTAG GAGGGTTTCC TGTGAAGAGT 
GTTGTTCCAG CTTTGATAAC ATTACTGCAG ATGGAACATA ATTTTGACAT TATGAATCAT 
GCTTGCCGAG CCTTAACATA TATGATGGAA GCTCTCCCCC GGTCATCTGC TGTTGTAGTG 
GATGCTATCC CTGTTTTCTT AGAAAAGTTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGCGACACA GTAAAGCCAT TTTACAAGCG 
GGTGGTTTGG CAGATTGCTT GTTGTATCTA GAATTCTTCA GCATCAATGC CCAAAGAAAT 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACAC CAGATGAGTT TCATTTTGTG 
GCAGATTCCC TACCATTGCT TACCCAAAGG CTAACTCATC AGGACAAAAA GTCAGTAGAA 
AGTACTTGCC TTTGTTTTGC ACGTTTAGTG GACAACTTTC AACATGAGGA GAACTTACTG 
CAACAGGTTG CTTCCAAAGA TCTACTTACA AATGTTCAAC AATTGTTGGT AGTGACTCCA 
CCGATTTTAA GTTCTGGGAT GTTTATTATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CATTAGCTGT TCAACTTATG AAACAAAGCT TTACAGACAT TGCAGAAACA 
CTTCACTTCC TGCTATGTGG TGCCTCCAAT GGAAGTTGTC AAGAGCAGAT TGACCTTGTT 
CCACGAAGTC CTCAAGAGCT GTATGAGCTG ACATCTTTGA TTTGTGAACT TATGCCATGT 
TTACCAAAAG AAGGCATCTT CGCAGTTGAT ACCATGTTGA AAAAGGGAAA TGCACAGAAC 
ACAGATGGTG CAATATGGCA GTGGCGGGAT GACCGAGGCC TCTGGCATCC CTATAACAGG 
ATTGACAGCC GGATCATTGA GGTAGCAGCC CATCAGGTCG GTGAGGATGA GATAAGCTTG 
TCCACTCTGG GACGAGTTTA TACTATTGAT TTTAATTCTA TGCAGCAAAT CAATGAAGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAATCCCT TAGCCAATTC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGATCCGGAA 
CTGGCTAAGT CTTTCATTAA GACCTTGTTT GGTGTTCTCT ATGAAGTGTA CAGCTCCTCA 
GCAGGCCCTG CGGTCAGACA CAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTTGCT 
GATGCTGAAC TTCTGAAGGA TGTTCTGAAA AATCATGCTG TTTCAAGTCA CATTGCTTCA 
ATGCTATCCA GCCAAGACCT GAAGATAGTA GTTGGAGCTC TTCAGATGGC AGAAATCCTA 
ATGCAAAAGT TACCTGATAT TTTTAGTGTT TACTTCAGGA GAGAAGGTGT GATGCATCAA 
GTCAAACATT TAGCAGAATC AGAGTCTTTG TTGACAAGTC CCCCCAAGGC ATGTACAAAT 
GGGTCAGGAT CCCTGGGATC TACAACACCA GCCAGCAGTG GGACTGCAAC GGCAGCAACC 
AACGCCTCTG CTGATCTGGG GTCACCTAGC TTGCAGCATA GCAGAGATGA TTCTTTGGAT 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTCAAGAGAA AACGACTGCC AAAACGAGGG 
CCAAGAAGGC CAAAATATTC CCCTCCAAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAATCT TCCTTCCTGG CAAGCTTGAA TCCAAAAACA 
TGGGGAAGGT TAAGCGCACA GTCCAACAGC AACAATATCG AACCAGCACG AACTGCAGGA 
GTTAGTGGTC TTGCCAGGGC AGCCTCAAAA GACACCATAT CCAATAATAG AGAAAAAATT 
AAAGGCTGGA TTAAGGAGCA GGCACATAAA TTTGTTGAGC GTTATTTTAG TTCTGAGAAT 
ATGGATGGAA GCAACCCTGC CTTGAATGTC CTTCAGAGAC TTTGTGCTGC AACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCAGATG TTTCTTCATT TGAAATCCAA CATAGTGGGT TTGTGAAACA ACTGTTGCTG 
TATTTGACAT CTAAGAATGA AAAGGATGCT GTCGGCAGAG AGATCCGGTT AAAACGTTTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CTGTTGGAAG AGTAGAACCA 
GTGGGCCATG CACCTTTGCT GGCATTGGTT CACAAGATGA ACAACTGTCT CAGCCAAATG 
GAGCAATTTC CAGTCAAAGT ACATGATTTC CCTAGTGGGA ACGGAGCTGG AGGAAGCTTT 
TCTCTCAACA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG TATTTTCCGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_017316627.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172116.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X18, mRNA.

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CloneID	OMu76980
Clone ID Related Accession (Same CDS sequence)	XM_017316627.2 , XM_017316627.1
Accession Version	XM_017316627.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CTCTGTTAGA GGCCAGGGGT CTTCCTCCTC ACCTATTTGG TCCTCTCGGG 
CCTCGGATGT CACAACTTTT CCATAGAACA ATTGGAAGCG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTTC AAGGACTACA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC CGTCATTGAA 
ATGTGTCAGT TATTGGTCAT GGGGAATGAG GAGACACTAG GAGGGTTTCC TGTGAAGAGT 
GTTGTTCCAG CTTTGATAAC ATTACTGCAG ATGGAACATA ATTTTGACAT TATGAATCAT 
GCTTGCCGAG CCTTAACATA TATGATGGAA GCTCTCCCCC GGTCATCTGC TGTTGTAGTG 
GATGCTATCC CTGTTTTCTT AGAAAAGTTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGCGACACA GTAAAGCCAT TTTACAAGCG 
GGTGGTTTGG CAGATTGCTT GTTGTATCTA GAATTCTTCA GCATCAATGC CCAAAGAAAT 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACAC CAGATGAGTT TCATTTTGTG 
GCAGATTCCC TACCATTGCT TACCCAAAGG CTAACTCATC AGGACAAAAA GTCAGTAGAA 
AGTACTTGCC TTTGTTTTGC ACGTTTAGTG GACAACTTTC AACATGAGGA GAACTTACTG 
CAACAGGTTG CTTCCAAAGA TCTACTTACA AATGTTCAAC AATTGTTGGT AGTGACTCCA 
CCGATTTTAA GTTCTGGGAT GTTTATTATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CATTAGCTGT TCAACTTATG AAACAAAGCT TTACAGACAT TGCAGAAACA 
CTTCACTTCC TGCTATGTGG TGCCTCCAAT GGAAGTTGTC AAGAGCAGAT TGACCTTGTT 
CCACGAAGTC CTCAAGAGCT GTATGAGCTG ACATCTTTGA TTTGTGAACT TATGCCATGT 
TTACCAAAAG AAGGCATCTT CGCAGTTGAT ACCATGTTGA AAAAGGGAAA TGCACAGAAC 
ACAGATGGTG CAATATGGCA GTGGCGGGAT GACCGAGGCC TCTGGCATCC CTATAACAGG 
ATTGACAGCC GGATCATTGA GGTAGCAGCC CATCAGGTCG GTGAGGATGA GATAAGCTTG 
TCCACTCTGG GACGAGTTTA TACTATTGAT TTTAATTCTA TGCAGCAAAT CAATGAAGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAATCCCT TAGCCAATTC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGATCCGGAA 
CTGGCTAAGT CTTTCATTAA GACCTTGTTT GGTGTTCTCT ATGAAGTGTA CAGCTCCTCA 
GCAGGCCCTG CGGTCAGACA CAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTTGCT 
GATGCTGAAC TTCTGAAGGA TGTTCTGAAA AATCATGCTG TTTCAAGTCA CATTGCTTCA 
ATGCTATCCA GCCAAGACCT GAAGATAGTA GTTGGAGCTC TTCAGATGGC AGAAATCCTA 
ATGCAAAAGT TACCTGATAT TTTTAGTGTT TACTTCAGGA GAGAAGGTGT GATGCATCAA 
GTCAAACATT TAGCAGAATC AGAGTCTTTG TTGACAAGTC CCCCCAAGGC ATGTACAAAT 
GGGTCAGGAT CCCTGGGATC TACAACACCA GCCAGCAGTG GGACTGCAAC GGCAGCAACC 
AACGCCTCTG CTGATCTGGG GTCACCTAGC TTGCAGCATA GCAGAGATGA TTCTTTGGAT 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTCAAGAGAA AACGACTGCC AAAACGAGGG 
CCAAGAAGGC CAAAATATTC CCCTCCAAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAATCT TCCTTCCTGG CAAGCTTGAA TCCAAAAACA 
TGGGGAAGGT TAAGCGCACA GTCCAACAGC AACAATATCG AACCAGCACG AACTGCAGGA 
GTTAGTGGTC TTGCCAGGGC AGCCTCAAAA GACACCATAT CCAATAATAG AGAAAAAATT 
AAAGGCTGGA TTAAGGAGCA GGCACATAAA TTTGTTGAGC GTTATTTTAG TTCTGAGAAT 
ATGGATGGAA GCAACCCTGC CTTGAATGTC CTTCAGAGAC TTTGTGCTGC AACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCAGATG TTTCTTCATT TGAAATCCAA CATAGTGGGT TTGTGAAACA ACTGTTGCTG 
TATTTGACAT CTAAGAATGA AAAGGATGCT GTCGGCAGAG AGATCCGGTT AAAACGTTTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CTGTTGGAAG AGTAGAACCA 
GTGGGCCATG CACCTTTGCT GGCATTGGTT CACAAGATGA ACAACTGTCT CAGCCAAATG 
GAGCAATTTC CAGTCAAAGT ACATGATTTC CCTAGTGGGA ACGGAGCTGG AGGAAGCTTT 
TCTCTCAACA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG TATTTTCCGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172116.1
CDS	400..6540
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017316627.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316627.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CTCTGTTAGA GGCCAGGGGT CTTCCTCCTC ACCTATTTGG TCCTCTCGGG 
CCTCGGATGT CACAACTTTT CCATAGAACA ATTGGAAGCG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTTC AAGGACTACA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC CGTCATTGAA 
ATGTGTCAGT TATTGGTCAT GGGGAATGAG GAGACACTAG GAGGGTTTCC TGTGAAGAGT 
GTTGTTCCAG CTTTGATAAC ATTACTGCAG ATGGAACATA ATTTTGACAT TATGAATCAT 
GCTTGCCGAG CCTTAACATA TATGATGGAA GCTCTCCCCC GGTCATCTGC TGTTGTAGTG 
GATGCTATCC CTGTTTTCTT AGAAAAGTTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGCGACACA GTAAAGCCAT TTTACAAGCG 
GGTGGTTTGG CAGATTGCTT GTTGTATCTA GAATTCTTCA GCATCAATGC CCAAAGAAAT 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACAC CAGATGAGTT TCATTTTGTG 
GCAGATTCCC TACCATTGCT TACCCAAAGG CTAACTCATC AGGACAAAAA GTCAGTAGAA 
AGTACTTGCC TTTGTTTTGC ACGTTTAGTG GACAACTTTC AACATGAGGA GAACTTACTG 
CAACAGGTTG CTTCCAAAGA TCTACTTACA AATGTTCAAC AATTGTTGGT AGTGACTCCA 
CCGATTTTAA GTTCTGGGAT GTTTATTATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CATTAGCTGT TCAACTTATG AAACAAAGCT TTACAGACAT TGCAGAAACA 
CTTCACTTCC TGCTATGTGG TGCCTCCAAT GGAAGTTGTC AAGAGCAGAT TGACCTTGTT 
CCACGAAGTC CTCAAGAGCT GTATGAGCTG ACATCTTTGA TTTGTGAACT TATGCCATGT 
TTACCAAAAG AAGGCATCTT CGCAGTTGAT ACCATGTTGA AAAAGGGAAA TGCACAGAAC 
ACAGATGGTG CAATATGGCA GTGGCGGGAT GACCGAGGCC TCTGGCATCC CTATAACAGG 
ATTGACAGCC GGATCATTGA GGTAGCAGCC CATCAGGTCG GTGAGGATGA GATAAGCTTG 
TCCACTCTGG GACGAGTTTA TACTATTGAT TTTAATTCTA TGCAGCAAAT CAATGAAGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAATCCCT TAGCCAATTC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGATCCGGAA 
CTGGCTAAGT CTTTCATTAA GACCTTGTTT GGTGTTCTCT ATGAAGTGTA CAGCTCCTCA 
GCAGGCCCTG CGGTCAGACA CAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTTGCT 
GATGCTGAAC TTCTGAAGGA TGTTCTGAAA AATCATGCTG TTTCAAGTCA CATTGCTTCA 
ATGCTATCCA GCCAAGACCT GAAGATAGTA GTTGGAGCTC TTCAGATGGC AGAAATCCTA 
ATGCAAAAGT TACCTGATAT TTTTAGTGTT TACTTCAGGA GAGAAGGTGT GATGCATCAA 
GTCAAACATT TAGCAGAATC AGAGTCTTTG TTGACAAGTC CCCCCAAGGC ATGTACAAAT 
GGGTCAGGAT CCCTGGGATC TACAACACCA GCCAGCAGTG GGACTGCAAC GGCAGCAACC 
AACGCCTCTG CTGATCTGGG GTCACCTAGC TTGCAGCATA GCAGAGATGA TTCTTTGGAT 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTCAAGAGAA AACGACTGCC AAAACGAGGG 
CCAAGAAGGC CAAAATATTC CCCTCCAAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAATCT TCCTTCCTGG CAAGCTTGAA TCCAAAAACA 
TGGGGAAGGT TAAGCGCACA GTCCAACAGC AACAATATCG AACCAGCACG AACTGCAGGA 
GTTAGTGGTC TTGCCAGGGC AGCCTCAAAA GACACCATAT CCAATAATAG AGAAAAAATT 
AAAGGCTGGA TTAAGGAGCA GGCACATAAA TTTGTTGAGC GTTATTTTAG TTCTGAGAAT 
ATGGATGGAA GCAACCCTGC CTTGAATGTC CTTCAGAGAC TTTGTGCTGC AACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCAGATG TTTCTTCATT TGAAATCCAA CATAGTGGGT TTGTGAAACA ACTGTTGCTG 
TATTTGACAT CTAAGAATGA AAAGGATGCT GTCGGCAGAG AGATCCGGTT AAAACGTTTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CTGTTGGAAG AGTAGAACCA 
GTGGGCCATG CACCTTTGCT GGCATTGGTT CACAAGATGA ACAACTGTCT CAGCCAAATG 
GAGCAATTTC CAGTCAAAGT ACATGATTTC CCTAGTGGGA ACGGAGCTGG AGGAAGCTTT 
TCTCTCAACA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG TATTTTCCGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_017316673.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172162.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X26, mRNA.

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CloneID	OMu76981
Clone ID Related Accession (Same CDS sequence)	XM_017316673.2 , XM_017316673.1
Accession Version	XM_017316673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CTCTGTTAGA GGCCAGGGGT CTTCCTCCTC ACCTATTTGG TCCTCTCGGG 
CCTCGGATGT CACAACTTTT CCATAGAACA ATTGGAAGCG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTTC AAGGACTACA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC CGTCATTGAA 
ATGTGTCAGT TATTGGTCAT GGGGAATGAG GAGACACTAG GAGGGTTTCC TGTGAAGAGT 
GTTGTTCCAG CTTTGATAAC ATTACTGCAG ATGGAACATA ATTTTGACAT TATGAATCAT 
GCTTGCCGAG CCTTAACATA TATGATGGAA GCTCTCCCCC GGTCATCTGC TGTTGTAGTG 
GATGCTATCC CTGTTTTCTT AGAAAAGTTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGCGACACA GTAAAGCCAT TTTACAAGCG 
GGTGGTTTGG CAGATTGCTT GTTGTATCTA GAATTCTTCA GCATCAATGC CCAAAGAAAT 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACAC CAGATGAGTT TCATTTTGTG 
GCAGATTCCC TACCATTGCT TACCCAAAGG CTAACTCATC AGGACAAAAA GTCAGTAGAA 
AGTACTTGCC TTTGTTTTGC ACGTTTAGTG GACAACTTTC AACATGAGGA GAACTTACTG 
CAACAGGTTG CTTCCAAAGA TCTACTTACA AATGTTCAAC AATTGTTGGT AGTGACTCCA 
CCGATTTTAA GTTCTGGGAT GTTTATTATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CATTAGCTGT TCAACTTATG AAACAAAACA TTGCAGAAAC ACTTCACTTC 
CTGCTATGTG GTGCCTCCAA TGGAAGTTGT CAAGAGCAGA TTGACCTTGT TCCACGAAGT 
CCTCAAGAGC TGTATGAGCT GACATCTTTG ATTTGTGAAC TTATGCCATG TTTACCAAAA 
GAAGGCATCT TCGCAGTTGA TACCATGTTG AAAAAGGGAA ATGCACAGAA CACAGATGGT 
GCAATATGGC AGTGGCGGGA TGACCGAGGC CTCTGGCATC CCTATAACAG GATTGACAGC 
CGGATCATTG AGGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAAGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAATCCCTTA GCCAATTCTA ACACTAGTGG ATATTCAGAG 
TTAAAGAAGG ATGATGCTCG AGCACAGCTA ATGAAAGAAG ATCCGGAACT GGCTAAGTCT 
TTCATTAAGA CCTTGTTTGG TGTTCTCTAT GAAGTGTACA GCTCCTCAGC AGGCCCTGCG 
GTCAGACACA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCTGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCAAT GCTATCCAGC 
CAAGACCTGA AGATAGTAGT TGGAGCTCTT CAGATGGCAG AAATCCTAAT GCAAAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGGAGA GAAGGTGTGA TGCATCAAGT CAAACATTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCC CCCAAGGCAT GTACAAATGG GTCAGGATCC 
CTGGGATCTA CAACACCAGC CAGCAGTGGG ACTGCAACGG CAGCAACCAA CGCCTCTGCT 
GATCTGGGGT CACCTAGCTT GCAGCATAGC AGAGATGATT CTTTGGATCT GAGCCCTCAA 
GGTCGATTAA GTGATGTTCT CAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAATATTCCC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC CTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGCGCACAGT CCAACAGCAA CAATATCGAA CCAGCACGAA CTGCAGGAGT TAGTGGTCTT 
GCCAGGGCAG CCTCAAAAGA CACCATATCC AATAATAGAG AAAAAATTAA AGGCTGGATT 
AAGGAGCAGG CACATAAATT TGTTGAGCGT TATTTTAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCCT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CTGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCTTCATTTG AAATCCAACA TAGTGGGTTT GTGAAACAAC TGTTGCTGTA TTTGACATCT 
AAGAATGAAA AGGATGCTGT CGGCAGAGAG ATCCGGTTAA AACGTTTCCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCT GTTGGAAGAG TAGAACCAGT GGGCCATGCA 
CCTTTGCTGG CATTGGTTCA CAAGATGAAC AACTGTCTCA GCCAAATGGA GCAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGGAAC GGAGCTGGAG GAAGCTTTTC TCTCAACAGA 
GGATCACAAG CTTTAAAATT TTTCAACACA CATCAGTTAA AATGTCAGTT ACAAAGACAC 
CCAGACTGTG CAAATGTGAA GCAGTGGAAA GGCGGGCCTG TCAAGATTGA TCCCCTGGCT 
CTGGTGCAAG CTATTGAGAG ATACCTTGTG GTTAGAGGAT ATGGAAGAGT AAGAGAAGAT 
GATGAAGATA GTGATGATGA TGGGTCAGAT GAAGAAATAG ATGAGTCTTT GGCTGCTCAA 
TTCTTAAATT CGGGAAATGT AAGGCACAGG CTACAGTTTT ATATTGGAGA ACATTTACTA 
CCATATAACA TGACCGTGTA TCAGGCAGTG CGGCAATTCA GTGTACAGGC TGAAGATGAA 
AGAGAATCTA CTGATGATGA GAGCAATCCT CTGGGAAGAG CTGGAATTTG GACAAAGACT 
CATACAATAT GGTACAAACC TGTGAGGGAG GATGAAGAAA GTACTAAAGA TTGTGTTGGT 
GGCAAAAGGG GGAGAGCCCA AACAGCTCCA ACAAAAACTT CCCCCAGAAA TGCAAAGAAG 
CATGATGAGT TATGGCATGA TGGAGTGTGC CCGTCAGTAG CAAATCCTTT AGAAGTTTAC 
CTCATTCCCA CACCACCTGA AAATATCACC TTTGAAGACC CATCCTTAGA TGTAATACTT 
CTTTTAAGAG TTTTACATGC CATCAGTCGA TATTGGTATT ACTTGTATGA TAATGCGATG 
TGCAAGGAGA TTATTCCAAC TAGTGAATTT ATTAACAGTA AATTAACAGC AAAAGCAAAT 
AGGCAGCTTC AAGACCCTCT AGTAATCATG ACAGGAAACA TCCCAACATG GCTCACTGAG 
CTAGGAAAAA CCTGCCCATT TTTCTTTCCT TTTGATACCC GACAAATGCT TTTTTATGTA 
ACTGCATTTG ATCGGGACCG GGCAATGCAA AGATTACTTG ATACCAACCC AGAAATCAAC 
CAGTCTGATT CTCAAGACAG CAGAGTTGCG CCTAGATTGG ATAGAAAAAA ACGTACTGTG 
AACAGAGAGG AGCTACTGAA ACAAGCTGAG TCTGTGATGC AGGACCTTGG CAGCTCTCGG 
GCCATGTTAG AAATCCAATA TGAAAATGAG GTTGGTACAG GTCTGGGGCC AACACTGGAG 
TTTTATGCAC TTGTTTCTCA GGAACTACAG AGAGCTGACT TGTGTCTCTG GAGGGGTGAA 
GAAGTAACTC TTAGCAATCC AAAAGGAAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG ACAGCTAAGC CAGCTCACAT CGCAAAGGTT 
AAGATGAAGT TTCGCTTCTT AGGAAAATTA ATGGCTAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CTCTTGGCCT GCCCTTTTAT AAGTGGATGC TGCGGCAGGA AACTTCATTG 
ACATCTCATG ATTTATTTGA CATTGATCCT GTTGTAGCCA GATCAGTATA TCACCTAGAA 
GACATTGTCA GACAGAAGAA AAGACTGGAG CAGGATAAAT CCCAGACCAA AGAGAGTCTA 
CAATATGCAT TGGAAACTCT TACGATGAAT GGCTGTTCAG TGGAAGATCT GGGATTGGAT 
TTTACGCTGC CTGGGTTTCC CAACATTGAA TTGAAGAAAG GAGGGAAGGA TATTCCAGTT 
ACTATCCACA ATTTGGAAGA GTACCTGAGA TTGGTGATAT TCTGGGCACT AAATGAAGGT 
GTTTGTCGAC AGTTTGACTC ATTCAGAGAT GGATTTGAAT CGGTCTTCCC GCTCTGCCAT 
CTTCAGTACT TCTACCCAGA AGAGCTGGAC CAGCTCCTGT GTGGCAGTAA AGCAGACACT 
TGGGATGCAA AGACATTGAT GGAATGCTGC AGGCCTGATC ATGGCTATAC TCATGACAGT 
CGGGCTGTGA AGTTCTTGTT TGAGATTCTC AGTAGTTTTG ATAATGAGCA GCAGAGGTTG 
TTTCTCCAGT TTGTGACTGG TAGCCCACGA TTGCCAGTTG GAGGATTTCG GAGTTTAAAT 
CCACCTCTGA CAATTGTGCG GAAGACATTT GAATCAACAG AAAACCCAGA TGACTTCTTA 
CCATCAGTAA TGACTTGTGT GAACTATCTT AAATTGCCGG ACTACTCGAG CATTGACATA 
ATGCGGGACA AACTCTTGAT AGCAGCAAGA GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172162.1
CDS	363..6482
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017316673.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316673.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CTCTGTTAGA GGCCAGGGGT CTTCCTCCTC ACCTATTTGG TCCTCTCGGG 
CCTCGGATGT CACAACTTTT CCATAGAACA ATTGGAAGCG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTTC AAGGACTACA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC CGTCATTGAA 
ATGTGTCAGT TATTGGTCAT GGGGAATGAG GAGACACTAG GAGGGTTTCC TGTGAAGAGT 
GTTGTTCCAG CTTTGATAAC ATTACTGCAG ATGGAACATA ATTTTGACAT TATGAATCAT 
GCTTGCCGAG CCTTAACATA TATGATGGAA GCTCTCCCCC GGTCATCTGC TGTTGTAGTG 
GATGCTATCC CTGTTTTCTT AGAAAAGTTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGCGACACA GTAAAGCCAT TTTACAAGCG 
GGTGGTTTGG CAGATTGCTT GTTGTATCTA GAATTCTTCA GCATCAATGC CCAAAGAAAT 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACAC CAGATGAGTT TCATTTTGTG 
GCAGATTCCC TACCATTGCT TACCCAAAGG CTAACTCATC AGGACAAAAA GTCAGTAGAA 
AGTACTTGCC TTTGTTTTGC ACGTTTAGTG GACAACTTTC AACATGAGGA GAACTTACTG 
CAACAGGTTG CTTCCAAAGA TCTACTTACA AATGTTCAAC AATTGTTGGT AGTGACTCCA 
CCGATTTTAA GTTCTGGGAT GTTTATTATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CATTAGCTGT TCAACTTATG AAACAAAACA TTGCAGAAAC ACTTCACTTC 
CTGCTATGTG GTGCCTCCAA TGGAAGTTGT CAAGAGCAGA TTGACCTTGT TCCACGAAGT 
CCTCAAGAGC TGTATGAGCT GACATCTTTG ATTTGTGAAC TTATGCCATG TTTACCAAAA 
GAAGGCATCT TCGCAGTTGA TACCATGTTG AAAAAGGGAA ATGCACAGAA CACAGATGGT 
GCAATATGGC AGTGGCGGGA TGACCGAGGC CTCTGGCATC CCTATAACAG GATTGACAGC 
CGGATCATTG AGGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAAGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAATCCCTTA GCCAATTCTA ACACTAGTGG ATATTCAGAG 
TTAAAGAAGG ATGATGCTCG AGCACAGCTA ATGAAAGAAG ATCCGGAACT GGCTAAGTCT 
TTCATTAAGA CCTTGTTTGG TGTTCTCTAT GAAGTGTACA GCTCCTCAGC AGGCCCTGCG 
GTCAGACACA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCTGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCAAT GCTATCCAGC 
CAAGACCTGA AGATAGTAGT TGGAGCTCTT CAGATGGCAG AAATCCTAAT GCAAAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGGAGA GAAGGTGTGA TGCATCAAGT CAAACATTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCC CCCAAGGCAT GTACAAATGG GTCAGGATCC 
CTGGGATCTA CAACACCAGC CAGCAGTGGG ACTGCAACGG CAGCAACCAA CGCCTCTGCT 
GATCTGGGGT CACCTAGCTT GCAGCATAGC AGAGATGATT CTTTGGATCT GAGCCCTCAA 
GGTCGATTAA GTGATGTTCT CAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAATATTCCC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC CTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGCGCACAGT CCAACAGCAA CAATATCGAA CCAGCACGAA CTGCAGGAGT TAGTGGTCTT 
GCCAGGGCAG CCTCAAAAGA CACCATATCC AATAATAGAG AAAAAATTAA AGGCTGGATT 
AAGGAGCAGG CACATAAATT TGTTGAGCGT TATTTTAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCCT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CTGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCTTCATTTG AAATCCAACA TAGTGGGTTT GTGAAACAAC TGTTGCTGTA TTTGACATCT 
AAGAATGAAA AGGATGCTGT CGGCAGAGAG ATCCGGTTAA AACGTTTCCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCT GTTGGAAGAG TAGAACCAGT GGGCCATGCA 
CCTTTGCTGG CATTGGTTCA CAAGATGAAC AACTGTCTCA GCCAAATGGA GCAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGGAAC GGAGCTGGAG GAAGCTTTTC TCTCAACAGA 
GGATCACAAG CTTTAAAATT TTTCAACACA CATCAGTTAA AATGTCAGTT ACAAAGACAC 
CCAGACTGTG CAAATGTGAA GCAGTGGAAA GGCGGGCCTG TCAAGATTGA TCCCCTGGCT 
CTGGTGCAAG CTATTGAGAG ATACCTTGTG GTTAGAGGAT ATGGAAGAGT AAGAGAAGAT 
GATGAAGATA GTGATGATGA TGGGTCAGAT GAAGAAATAG ATGAGTCTTT GGCTGCTCAA 
TTCTTAAATT CGGGAAATGT AAGGCACAGG CTACAGTTTT ATATTGGAGA ACATTTACTA 
CCATATAACA TGACCGTGTA TCAGGCAGTG CGGCAATTCA GTGTACAGGC TGAAGATGAA 
AGAGAATCTA CTGATGATGA GAGCAATCCT CTGGGAAGAG CTGGAATTTG GACAAAGACT 
CATACAATAT GGTACAAACC TGTGAGGGAG GATGAAGAAA GTACTAAAGA TTGTGTTGGT 
GGCAAAAGGG GGAGAGCCCA AACAGCTCCA ACAAAAACTT CCCCCAGAAA TGCAAAGAAG 
CATGATGAGT TATGGCATGA TGGAGTGTGC CCGTCAGTAG CAAATCCTTT AGAAGTTTAC 
CTCATTCCCA CACCACCTGA AAATATCACC TTTGAAGACC CATCCTTAGA TGTAATACTT 
CTTTTAAGAG TTTTACATGC CATCAGTCGA TATTGGTATT ACTTGTATGA TAATGCGATG 
TGCAAGGAGA TTATTCCAAC TAGTGAATTT ATTAACAGTA AATTAACAGC AAAAGCAAAT 
AGGCAGCTTC AAGACCCTCT AGTAATCATG ACAGGAAACA TCCCAACATG GCTCACTGAG 
CTAGGAAAAA CCTGCCCATT TTTCTTTCCT TTTGATACCC GACAAATGCT TTTTTATGTA 
ACTGCATTTG ATCGGGACCG GGCAATGCAA AGATTACTTG ATACCAACCC AGAAATCAAC 
CAGTCTGATT CTCAAGACAG CAGAGTTGCG CCTAGATTGG ATAGAAAAAA ACGTACTGTG 
AACAGAGAGG AGCTACTGAA ACAAGCTGAG TCTGTGATGC AGGACCTTGG CAGCTCTCGG 
GCCATGTTAG AAATCCAATA TGAAAATGAG GTTGGTACAG GTCTGGGGCC AACACTGGAG 
TTTTATGCAC TTGTTTCTCA GGAACTACAG AGAGCTGACT TGTGTCTCTG GAGGGGTGAA 
GAAGTAACTC TTAGCAATCC AAAAGGAAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG ACAGCTAAGC CAGCTCACAT CGCAAAGGTT 
AAGATGAAGT TTCGCTTCTT AGGAAAATTA ATGGCTAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CTCTTGGCCT GCCCTTTTAT AAGTGGATGC TGCGGCAGGA AACTTCATTG 
ACATCTCATG ATTTATTTGA CATTGATCCT GTTGTAGCCA GATCAGTATA TCACCTAGAA 
GACATTGTCA GACAGAAGAA AAGACTGGAG CAGGATAAAT CCCAGACCAA AGAGAGTCTA 
CAATATGCAT TGGAAACTCT TACGATGAAT GGCTGTTCAG TGGAAGATCT GGGATTGGAT 
TTTACGCTGC CTGGGTTTCC CAACATTGAA TTGAAGAAAG GAGGGAAGGA TATTCCAGTT 
ACTATCCACA ATTTGGAAGA GTACCTGAGA TTGGTGATAT TCTGGGCACT AAATGAAGGT 
GTTTGTCGAC AGTTTGACTC ATTCAGAGAT GGATTTGAAT CGGTCTTCCC GCTCTGCCAT 
CTTCAGTACT TCTACCCAGA AGAGCTGGAC CAGCTCCTGT GTGGCAGTAA AGCAGACACT 
TGGGATGCAA AGACATTGAT GGAATGCTGC AGGCCTGATC ATGGCTATAC TCATGACAGT 
CGGGCTGTGA AGTTCTTGTT TGAGATTCTC AGTAGTTTTG ATAATGAGCA GCAGAGGTTG 
TTTCTCCAGT TTGTGACTGG TAGCCCACGA TTGCCAGTTG GAGGATTTCG GAGTTTAAAT 
CCACCTCTGA CAATTGTGCG GAAGACATTT GAATCAACAG AAAACCCAGA TGACTTCTTA 
CCATCAGTAA TGACTTGTGT GAACTATCTT AAATTGCCGG ACTACTCGAG CATTGACATA 
ATGCGGGACA AACTCTTGAT AGCAGCAAGA GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_017316673.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172162.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X21, mRNA.

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CloneID	OMu76981
Clone ID Related Accession (Same CDS sequence)	XM_017316673.2 , XM_017316673.1
Accession Version	XM_017316673.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CTCTGTTAGA GGCCAGGGGT CTTCCTCCTC ACCTATTTGG TCCTCTCGGG 
CCTCGGATGT CACAACTTTT CCATAGAACA ATTGGAAGCG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTTC AAGGACTACA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC CGTCATTGAA 
ATGTGTCAGT TATTGGTCAT GGGGAATGAG GAGACACTAG GAGGGTTTCC TGTGAAGAGT 
GTTGTTCCAG CTTTGATAAC ATTACTGCAG ATGGAACATA ATTTTGACAT TATGAATCAT 
GCTTGCCGAG CCTTAACATA TATGATGGAA GCTCTCCCCC GGTCATCTGC TGTTGTAGTG 
GATGCTATCC CTGTTTTCTT AGAAAAGTTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGCGACACA GTAAAGCCAT TTTACAAGCG 
GGTGGTTTGG CAGATTGCTT GTTGTATCTA GAATTCTTCA GCATCAATGC CCAAAGAAAT 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACAC CAGATGAGTT TCATTTTGTG 
GCAGATTCCC TACCATTGCT TACCCAAAGG CTAACTCATC AGGACAAAAA GTCAGTAGAA 
AGTACTTGCC TTTGTTTTGC ACGTTTAGTG GACAACTTTC AACATGAGGA GAACTTACTG 
CAACAGGTTG CTTCCAAAGA TCTACTTACA AATGTTCAAC AATTGTTGGT AGTGACTCCA 
CCGATTTTAA GTTCTGGGAT GTTTATTATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CATTAGCTGT TCAACTTATG AAACAAAACA TTGCAGAAAC ACTTCACTTC 
CTGCTATGTG GTGCCTCCAA TGGAAGTTGT CAAGAGCAGA TTGACCTTGT TCCACGAAGT 
CCTCAAGAGC TGTATGAGCT GACATCTTTG ATTTGTGAAC TTATGCCATG TTTACCAAAA 
GAAGGCATCT TCGCAGTTGA TACCATGTTG AAAAAGGGAA ATGCACAGAA CACAGATGGT 
GCAATATGGC AGTGGCGGGA TGACCGAGGC CTCTGGCATC CCTATAACAG GATTGACAGC 
CGGATCATTG AGGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAAGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAATCCCTTA GCCAATTCTA ACACTAGTGG ATATTCAGAG 
TTAAAGAAGG ATGATGCTCG AGCACAGCTA ATGAAAGAAG ATCCGGAACT GGCTAAGTCT 
TTCATTAAGA CCTTGTTTGG TGTTCTCTAT GAAGTGTACA GCTCCTCAGC AGGCCCTGCG 
GTCAGACACA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCTGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCAAT GCTATCCAGC 
CAAGACCTGA AGATAGTAGT TGGAGCTCTT CAGATGGCAG AAATCCTAAT GCAAAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGGAGA GAAGGTGTGA TGCATCAAGT CAAACATTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCC CCCAAGGCAT GTACAAATGG GTCAGGATCC 
CTGGGATCTA CAACACCAGC CAGCAGTGGG ACTGCAACGG CAGCAACCAA CGCCTCTGCT 
GATCTGGGGT CACCTAGCTT GCAGCATAGC AGAGATGATT CTTTGGATCT GAGCCCTCAA 
GGTCGATTAA GTGATGTTCT CAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAATATTCCC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC CTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGCGCACAGT CCAACAGCAA CAATATCGAA CCAGCACGAA CTGCAGGAGT TAGTGGTCTT 
GCCAGGGCAG CCTCAAAAGA CACCATATCC AATAATAGAG AAAAAATTAA AGGCTGGATT 
AAGGAGCAGG CACATAAATT TGTTGAGCGT TATTTTAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCCT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CTGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCTTCATTTG AAATCCAACA TAGTGGGTTT GTGAAACAAC TGTTGCTGTA TTTGACATCT 
AAGAATGAAA AGGATGCTGT CGGCAGAGAG ATCCGGTTAA AACGTTTCCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCT GTTGGAAGAG TAGAACCAGT GGGCCATGCA 
CCTTTGCTGG CATTGGTTCA CAAGATGAAC AACTGTCTCA GCCAAATGGA GCAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGGAAC GGAGCTGGAG GAAGCTTTTC TCTCAACAGA 
GGATCACAAG CTTTAAAATT TTTCAACACA CATCAGTTAA AATGTCAGTT ACAAAGACAC 
CCAGACTGTG CAAATGTGAA GCAGTGGAAA GGCGGGCCTG TCAAGATTGA TCCCCTGGCT 
CTGGTGCAAG CTATTGAGAG ATACCTTGTG GTTAGAGGAT ATGGAAGAGT AAGAGAAGAT 
GATGAAGATA GTGATGATGA TGGGTCAGAT GAAGAAATAG ATGAGTCTTT GGCTGCTCAA 
TTCTTAAATT CGGGAAATGT AAGGCACAGG CTACAGTTTT ATATTGGAGA ACATTTACTA 
CCATATAACA TGACCGTGTA TCAGGCAGTG CGGCAATTCA GTGTACAGGC TGAAGATGAA 
AGAGAATCTA CTGATGATGA GAGCAATCCT CTGGGAAGAG CTGGAATTTG GACAAAGACT 
CATACAATAT GGTACAAACC TGTGAGGGAG GATGAAGAAA GTACTAAAGA TTGTGTTGGT 
GGCAAAAGGG GGAGAGCCCA AACAGCTCCA ACAAAAACTT CCCCCAGAAA TGCAAAGAAG 
CATGATGAGT TATGGCATGA TGGAGTGTGC CCGTCAGTAG CAAATCCTTT AGAAGTTTAC 
CTCATTCCCA CACCACCTGA AAATATCACC TTTGAAGACC CATCCTTAGA TGTAATACTT 
CTTTTAAGAG TTTTACATGC CATCAGTCGA TATTGGTATT ACTTGTATGA TAATGCGATG 
TGCAAGGAGA TTATTCCAAC TAGTGAATTT ATTAACAGTA AATTAACAGC AAAAGCAAAT 
AGGCAGCTTC AAGACCCTCT AGTAATCATG ACAGGAAACA TCCCAACATG GCTCACTGAG 
CTAGGAAAAA CCTGCCCATT TTTCTTTCCT TTTGATACCC GACAAATGCT TTTTTATGTA 
ACTGCATTTG ATCGGGACCG GGCAATGCAA AGATTACTTG ATACCAACCC AGAAATCAAC 
CAGTCTGATT CTCAAGACAG CAGAGTTGCG CCTAGATTGG ATAGAAAAAA ACGTACTGTG 
AACAGAGAGG AGCTACTGAA ACAAGCTGAG TCTGTGATGC AGGACCTTGG CAGCTCTCGG 
GCCATGTTAG AAATCCAATA TGAAAATGAG GTTGGTACAG GTCTGGGGCC AACACTGGAG 
TTTTATGCAC TTGTTTCTCA GGAACTACAG AGAGCTGACT TGTGTCTCTG GAGGGGTGAA 
GAAGTAACTC TTAGCAATCC AAAAGGAAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG ACAGCTAAGC CAGCTCACAT CGCAAAGGTT 
AAGATGAAGT TTCGCTTCTT AGGAAAATTA ATGGCTAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CTCTTGGCCT GCCCTTTTAT AAGTGGATGC TGCGGCAGGA AACTTCATTG 
ACATCTCATG ATTTATTTGA CATTGATCCT GTTGTAGCCA GATCAGTATA TCACCTAGAA 
GACATTGTCA GACAGAAGAA AAGACTGGAG CAGGATAAAT CCCAGACCAA AGAGAGTCTA 
CAATATGCAT TGGAAACTCT TACGATGAAT GGCTGTTCAG TGGAAGATCT GGGATTGGAT 
TTTACGCTGC CTGGGTTTCC CAACATTGAA TTGAAGAAAG GAGGGAAGGA TATTCCAGTT 
ACTATCCACA ATTTGGAAGA GTACCTGAGA TTGGTGATAT TCTGGGCACT AAATGAAGGT 
GTTTGTCGAC AGTTTGACTC ATTCAGAGAT GGATTTGAAT CGGTCTTCCC GCTCTGCCAT 
CTTCAGTACT TCTACCCAGA AGAGCTGGAC CAGCTCCTGT GTGGCAGTAA AGCAGACACT 
TGGGATGCAA AGACATTGAT GGAATGCTGC AGGCCTGATC ATGGCTATAC TCATGACAGT 
CGGGCTGTGA AGTTCTTGTT TGAGATTCTC AGTAGTTTTG ATAATGAGCA GCAGAGGTTG 
TTTCTCCAGT TTGTGACTGG TAGCCCACGA TTGCCAGTTG GAGGATTTCG GAGTTTAAAT 
CCACCTCTGA CAATTGTGCG GAAGACATTT GAATCAACAG AAAACCCAGA TGACTTCTTA 
CCATCAGTAA TGACTTGTGT GAACTATCTT AAATTGCCGG ACTACTCGAG CATTGACATA 
ATGCGGGACA AACTCTTGAT AGCAGCAAGA GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172162.1
CDS	400..6519
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017316673.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316673.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CTCTGTTAGA GGCCAGGGGT CTTCCTCCTC ACCTATTTGG TCCTCTCGGG 
CCTCGGATGT CACAACTTTT CCATAGAACA ATTGGAAGCG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTTC AAGGACTACA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC CGTCATTGAA 
ATGTGTCAGT TATTGGTCAT GGGGAATGAG GAGACACTAG GAGGGTTTCC TGTGAAGAGT 
GTTGTTCCAG CTTTGATAAC ATTACTGCAG ATGGAACATA ATTTTGACAT TATGAATCAT 
GCTTGCCGAG CCTTAACATA TATGATGGAA GCTCTCCCCC GGTCATCTGC TGTTGTAGTG 
GATGCTATCC CTGTTTTCTT AGAAAAGTTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGCGACACA GTAAAGCCAT TTTACAAGCG 
GGTGGTTTGG CAGATTGCTT GTTGTATCTA GAATTCTTCA GCATCAATGC CCAAAGAAAT 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACAC CAGATGAGTT TCATTTTGTG 
GCAGATTCCC TACCATTGCT TACCCAAAGG CTAACTCATC AGGACAAAAA GTCAGTAGAA 
AGTACTTGCC TTTGTTTTGC ACGTTTAGTG GACAACTTTC AACATGAGGA GAACTTACTG 
CAACAGGTTG CTTCCAAAGA TCTACTTACA AATGTTCAAC AATTGTTGGT AGTGACTCCA 
CCGATTTTAA GTTCTGGGAT GTTTATTATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CATTAGCTGT TCAACTTATG AAACAAAACA TTGCAGAAAC ACTTCACTTC 
CTGCTATGTG GTGCCTCCAA TGGAAGTTGT CAAGAGCAGA TTGACCTTGT TCCACGAAGT 
CCTCAAGAGC TGTATGAGCT GACATCTTTG ATTTGTGAAC TTATGCCATG TTTACCAAAA 
GAAGGCATCT TCGCAGTTGA TACCATGTTG AAAAAGGGAA ATGCACAGAA CACAGATGGT 
GCAATATGGC AGTGGCGGGA TGACCGAGGC CTCTGGCATC CCTATAACAG GATTGACAGC 
CGGATCATTG AGGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAAGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAATCCCTTA GCCAATTCTA ACACTAGTGG ATATTCAGAG 
TTAAAGAAGG ATGATGCTCG AGCACAGCTA ATGAAAGAAG ATCCGGAACT GGCTAAGTCT 
TTCATTAAGA CCTTGTTTGG TGTTCTCTAT GAAGTGTACA GCTCCTCAGC AGGCCCTGCG 
GTCAGACACA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCTGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCAAT GCTATCCAGC 
CAAGACCTGA AGATAGTAGT TGGAGCTCTT CAGATGGCAG AAATCCTAAT GCAAAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGGAGA GAAGGTGTGA TGCATCAAGT CAAACATTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCC CCCAAGGCAT GTACAAATGG GTCAGGATCC 
CTGGGATCTA CAACACCAGC CAGCAGTGGG ACTGCAACGG CAGCAACCAA CGCCTCTGCT 
GATCTGGGGT CACCTAGCTT GCAGCATAGC AGAGATGATT CTTTGGATCT GAGCCCTCAA 
GGTCGATTAA GTGATGTTCT CAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAATATTCCC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC CTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGCGCACAGT CCAACAGCAA CAATATCGAA CCAGCACGAA CTGCAGGAGT TAGTGGTCTT 
GCCAGGGCAG CCTCAAAAGA CACCATATCC AATAATAGAG AAAAAATTAA AGGCTGGATT 
AAGGAGCAGG CACATAAATT TGTTGAGCGT TATTTTAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCCT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CTGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCTTCATTTG AAATCCAACA TAGTGGGTTT GTGAAACAAC TGTTGCTGTA TTTGACATCT 
AAGAATGAAA AGGATGCTGT CGGCAGAGAG ATCCGGTTAA AACGTTTCCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCT GTTGGAAGAG TAGAACCAGT GGGCCATGCA 
CCTTTGCTGG CATTGGTTCA CAAGATGAAC AACTGTCTCA GCCAAATGGA GCAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGGAAC GGAGCTGGAG GAAGCTTTTC TCTCAACAGA 
GGATCACAAG CTTTAAAATT TTTCAACACA CATCAGTTAA AATGTCAGTT ACAAAGACAC 
CCAGACTGTG CAAATGTGAA GCAGTGGAAA GGCGGGCCTG TCAAGATTGA TCCCCTGGCT 
CTGGTGCAAG CTATTGAGAG ATACCTTGTG GTTAGAGGAT ATGGAAGAGT AAGAGAAGAT 
GATGAAGATA GTGATGATGA TGGGTCAGAT GAAGAAATAG ATGAGTCTTT GGCTGCTCAA 
TTCTTAAATT CGGGAAATGT AAGGCACAGG CTACAGTTTT ATATTGGAGA ACATTTACTA 
CCATATAACA TGACCGTGTA TCAGGCAGTG CGGCAATTCA GTGTACAGGC TGAAGATGAA 
AGAGAATCTA CTGATGATGA GAGCAATCCT CTGGGAAGAG CTGGAATTTG GACAAAGACT 
CATACAATAT GGTACAAACC TGTGAGGGAG GATGAAGAAA GTACTAAAGA TTGTGTTGGT 
GGCAAAAGGG GGAGAGCCCA AACAGCTCCA ACAAAAACTT CCCCCAGAAA TGCAAAGAAG 
CATGATGAGT TATGGCATGA TGGAGTGTGC CCGTCAGTAG CAAATCCTTT AGAAGTTTAC 
CTCATTCCCA CACCACCTGA AAATATCACC TTTGAAGACC CATCCTTAGA TGTAATACTT 
CTTTTAAGAG TTTTACATGC CATCAGTCGA TATTGGTATT ACTTGTATGA TAATGCGATG 
TGCAAGGAGA TTATTCCAAC TAGTGAATTT ATTAACAGTA AATTAACAGC AAAAGCAAAT 
AGGCAGCTTC AAGACCCTCT AGTAATCATG ACAGGAAACA TCCCAACATG GCTCACTGAG 
CTAGGAAAAA CCTGCCCATT TTTCTTTCCT TTTGATACCC GACAAATGCT TTTTTATGTA 
ACTGCATTTG ATCGGGACCG GGCAATGCAA AGATTACTTG ATACCAACCC AGAAATCAAC 
CAGTCTGATT CTCAAGACAG CAGAGTTGCG CCTAGATTGG ATAGAAAAAA ACGTACTGTG 
AACAGAGAGG AGCTACTGAA ACAAGCTGAG TCTGTGATGC AGGACCTTGG CAGCTCTCGG 
GCCATGTTAG AAATCCAATA TGAAAATGAG GTTGGTACAG GTCTGGGGCC AACACTGGAG 
TTTTATGCAC TTGTTTCTCA GGAACTACAG AGAGCTGACT TGTGTCTCTG GAGGGGTGAA 
GAAGTAACTC TTAGCAATCC AAAAGGAAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG ACAGCTAAGC CAGCTCACAT CGCAAAGGTT 
AAGATGAAGT TTCGCTTCTT AGGAAAATTA ATGGCTAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CTCTTGGCCT GCCCTTTTAT AAGTGGATGC TGCGGCAGGA AACTTCATTG 
ACATCTCATG ATTTATTTGA CATTGATCCT GTTGTAGCCA GATCAGTATA TCACCTAGAA 
GACATTGTCA GACAGAAGAA AAGACTGGAG CAGGATAAAT CCCAGACCAA AGAGAGTCTA 
CAATATGCAT TGGAAACTCT TACGATGAAT GGCTGTTCAG TGGAAGATCT GGGATTGGAT 
TTTACGCTGC CTGGGTTTCC CAACATTGAA TTGAAGAAAG GAGGGAAGGA TATTCCAGTT 
ACTATCCACA ATTTGGAAGA GTACCTGAGA TTGGTGATAT TCTGGGCACT AAATGAAGGT 
GTTTGTCGAC AGTTTGACTC ATTCAGAGAT GGATTTGAAT CGGTCTTCCC GCTCTGCCAT 
CTTCAGTACT TCTACCCAGA AGAGCTGGAC CAGCTCCTGT GTGGCAGTAA AGCAGACACT 
TGGGATGCAA AGACATTGAT GGAATGCTGC AGGCCTGATC ATGGCTATAC TCATGACAGT 
CGGGCTGTGA AGTTCTTGTT TGAGATTCTC AGTAGTTTTG ATAATGAGCA GCAGAGGTTG 
TTTCTCCAGT TTGTGACTGG TAGCCCACGA TTGCCAGTTG GAGGATTTCG GAGTTTAAAT 
CCACCTCTGA CAATTGTGCG GAAGACATTT GAATCAACAG AAAACCCAGA TGACTTCTTA 
CCATCAGTAA TGACTTGTGT GAACTATCTT AAATTGCCGG ACTACTCGAG CATTGACATA 
ATGCGGGACA AACTCTTGAT AGCAGCAAGA GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030248895.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104755.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X14, mRNA.

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CloneID	OMu85010
Clone ID Related Accession (Same CDS sequence)	XM_030248895.1
Accession Version	XM_030248895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AAGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA TCCCTTAGCC 
AATTCTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTAATG 
AAAGAAGATC CGGAACTGGC TAAGTCTTTC ATTAAGACCT TGTTTGGTGT TCTCTATGAA 
GTGTACAGCT CCTCAGCAGG CCCTGCGGTC AGACACAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCTGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCAATGCT ATCCAGCCAA GACCTGAAGA TAGTAGTTGG AGCTCTTCAG 
ATGGCAGAAA TCCTAATGCA AAAGTTACCT GATATTTTTA GTGTTTACTT CAGGAGAGAA 
GGTGTGATGC ATCAAGTCAA ACATTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCCCCC 
AAGGCATGTA CAAATGGGTC AGGATCCCTG GGATCTACAA CACCAGCCAG CAGTGGGACT 
GCAACGGCAG CAACCAACGC CTCTGCTGAT CTGGGGTCAC CTAGCTTGCA GCATAGCAGA 
GATGATTCTT TGGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTCAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA TATTCCCCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCCTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA TATCGAACCA 
GCACGAACTG CAGGAGTTAG TGGTCTTGCC AGGGCAGCCT CAAAAGACAC CATATCCAAT 
AATAGAGAAA AAATTAAAGG CTGGATTAAG GAGCAGGCAC ATAAATTTGT TGAGCGTTAT 
TTTAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCCTTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCT TCATTTGAAA TCCAACATAG TGGGTTTGTG 
AAACAACTGT TGCTGTATTT GACATCTAAG AATGAAAAGG ATGCTGTCGG CAGAGAGATC 
CGGTTAAAAC GTTTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT 
GGAAGAGTAG AACCAGTGGG CCATGCACCT TTGCTGGCAT TGGTTCACAA GATGAACAAC 
TGTCTCAGCC AAATGGAGCA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGGAACGGA 
GCTGGAGGAA GCTTTTCTCT CAACAGAGGA TCACAAGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GTCAGTTACA AAGACACCCA GACTGTGCAA ATGTGAAGCA GTGGAAAGGC 
GGGCCTGTCA AGATTGATCC CCTGGCTCTG GTGCAAGCTA TTGAGAGATA CCTTGTGGTT 
AGAGGATATG GAAGAGTAAG AGAAGATGAT GAAGATAGTG ATGATGATGG GTCAGATGAA 
GAAATAGATG AGTCTTTGGC TGCTCAATTC TTAAATTCGG GAAATGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTACTACCA TATAACATGA CCGTGTATCA GGCAGTGCGG 
CAATTCAGTG TACAGGCTGA AGATGAAAGA GAATCTACTG ATGATGAGAG CAATCCTCTG 
GGAAGAGCTG GAATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGGGAGGAT 
GAAGAAAGTA CTAAAGATTG TGTTGGTGGC AAAAGGGGGA GAGCCCAAAC AGCTCCAACA 
AAAACTTCCC CCAGAAATGC AAAGAAGCAT GATGAGTTAT GGCATGATGG AGTGTGCCCG 
TCAGTAGCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATCACCTTT 
GAAGACCCAT CCTTAGATGT AATACTTCTT TTAAGAGTTT TACATGCCAT CAGTCGATAT 
TGGTATTACT TGTATGATAA TGCGATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAAT TAACAGCAAA AGCAAATAGG CAGCTTCAAG ACCCTCTAGT AATCATGACA 
GGAAACATCC CAACATGGCT CACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGAC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGGGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGACAGCAG AGTTGCGCCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TACTGAAACA AGCTGAGTCT 
GTGATGCAGG ACCTTGGCAG CTCTCGGGCC ATGTTAGAAA TCCAATATGA AAATGAGGTT 
GGTACAGGTC TGGGGCCAAC ACTGGAGTTT TATGCACTTG TTTCTCAGGA ACTACAGAGA 
GCTGACTTGT GTCTCTGGAG GGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCACATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCTAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCTC TTGGCCTGCC CTTTTATAAG 
TGGATGCTGC GGCAGGAAAC TTCATTGACA TCTCATGATT TATTTGACAT TGATCCTGTT 
GTAGCCAGAT CAGTATATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTGGAGCAG 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTGG AAACTCTTAC GATGAATGGC 
TGTTCAGTGG AAGATCTGGG ATTGGATTTT ACGCTGCCTG GGTTTCCCAA CATTGAATTG 
AAGAAAGGAG GGAAGGATAT TCCAGTTACT ATCCACAATT TGGAAGAGTA CCTGAGATTG 
GTGATATTCT GGGCACTAAA TGAAGGTGTT TGTCGACAGT TTGACTCATT CAGAGATGGA 
TTTGAATCGG TCTTCCCGCT CTGCCATCTT CAGTACTTCT ACCCAGAAGA GCTGGACCAG 
CTCCTGTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CATTGATGGA ATGCTGCAGG 
CCTGATCATG GCTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACTGGTAG CCCACGATTG 
CCAGTTGGAG GATTTCGGAG TTTAAATCCA CCTCTGACAA TTGTGCGGAA GACATTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTACCA TCAGTAATGA CTTGTGTGAA CTATCTTAAA 
TTGCCGGACT ACTCGAGCAT TGACATAATG CGGGACAAAC TCTTGATAGC AGCAAGAGAA 
GGCCAACAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104755.1
CDS	363..6569
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030248895.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248895.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AAGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA TCCCTTAGCC 
AATTCTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTAATG 
AAAGAAGATC CGGAACTGGC TAAGTCTTTC ATTAAGACCT TGTTTGGTGT TCTCTATGAA 
GTGTACAGCT CCTCAGCAGG CCCTGCGGTC AGACACAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCTGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCAATGCT ATCCAGCCAA GACCTGAAGA TAGTAGTTGG AGCTCTTCAG 
ATGGCAGAAA TCCTAATGCA AAAGTTACCT GATATTTTTA GTGTTTACTT CAGGAGAGAA 
GGTGTGATGC ATCAAGTCAA ACATTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCCCCC 
AAGGCATGTA CAAATGGGTC AGGATCCCTG GGATCTACAA CACCAGCCAG CAGTGGGACT 
GCAACGGCAG CAACCAACGC CTCTGCTGAT CTGGGGTCAC CTAGCTTGCA GCATAGCAGA 
GATGATTCTT TGGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTCAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA TATTCCCCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCCTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA TATCGAACCA 
GCACGAACTG CAGGAGTTAG TGGTCTTGCC AGGGCAGCCT CAAAAGACAC CATATCCAAT 
AATAGAGAAA AAATTAAAGG CTGGATTAAG GAGCAGGCAC ATAAATTTGT TGAGCGTTAT 
TTTAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCCTTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCT TCATTTGAAA TCCAACATAG TGGGTTTGTG 
AAACAACTGT TGCTGTATTT GACATCTAAG AATGAAAAGG ATGCTGTCGG CAGAGAGATC 
CGGTTAAAAC GTTTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT 
GGAAGAGTAG AACCAGTGGG CCATGCACCT TTGCTGGCAT TGGTTCACAA GATGAACAAC 
TGTCTCAGCC AAATGGAGCA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGGAACGGA 
GCTGGAGGAA GCTTTTCTCT CAACAGAGGA TCACAAGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GTCAGTTACA AAGACACCCA GACTGTGCAA ATGTGAAGCA GTGGAAAGGC 
GGGCCTGTCA AGATTGATCC CCTGGCTCTG GTGCAAGCTA TTGAGAGATA CCTTGTGGTT 
AGAGGATATG GAAGAGTAAG AGAAGATGAT GAAGATAGTG ATGATGATGG GTCAGATGAA 
GAAATAGATG AGTCTTTGGC TGCTCAATTC TTAAATTCGG GAAATGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTACTACCA TATAACATGA CCGTGTATCA GGCAGTGCGG 
CAATTCAGTG TACAGGCTGA AGATGAAAGA GAATCTACTG ATGATGAGAG CAATCCTCTG 
GGAAGAGCTG GAATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGGGAGGAT 
GAAGAAAGTA CTAAAGATTG TGTTGGTGGC AAAAGGGGGA GAGCCCAAAC AGCTCCAACA 
AAAACTTCCC CCAGAAATGC AAAGAAGCAT GATGAGTTAT GGCATGATGG AGTGTGCCCG 
TCAGTAGCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATCACCTTT 
GAAGACCCAT CCTTAGATGT AATACTTCTT TTAAGAGTTT TACATGCCAT CAGTCGATAT 
TGGTATTACT TGTATGATAA TGCGATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAAT TAACAGCAAA AGCAAATAGG CAGCTTCAAG ACCCTCTAGT AATCATGACA 
GGAAACATCC CAACATGGCT CACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGAC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGGGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGACAGCAG AGTTGCGCCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TACTGAAACA AGCTGAGTCT 
GTGATGCAGG ACCTTGGCAG CTCTCGGGCC ATGTTAGAAA TCCAATATGA AAATGAGGTT 
GGTACAGGTC TGGGGCCAAC ACTGGAGTTT TATGCACTTG TTTCTCAGGA ACTACAGAGA 
GCTGACTTGT GTCTCTGGAG GGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCACATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCTAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCTC TTGGCCTGCC CTTTTATAAG 
TGGATGCTGC GGCAGGAAAC TTCATTGACA TCTCATGATT TATTTGACAT TGATCCTGTT 
GTAGCCAGAT CAGTATATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTGGAGCAG 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTGG AAACTCTTAC GATGAATGGC 
TGTTCAGTGG AAGATCTGGG ATTGGATTTT ACGCTGCCTG GGTTTCCCAA CATTGAATTG 
AAGAAAGGAG GGAAGGATAT TCCAGTTACT ATCCACAATT TGGAAGAGTA CCTGAGATTG 
GTGATATTCT GGGCACTAAA TGAAGGTGTT TGTCGACAGT TTGACTCATT CAGAGATGGA 
TTTGAATCGG TCTTCCCGCT CTGCCATCTT CAGTACTTCT ACCCAGAAGA GCTGGACCAG 
CTCCTGTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CATTGATGGA ATGCTGCAGG 
CCTGATCATG GCTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACTGGTAG CCCACGATTG 
CCAGTTGGAG GATTTCGGAG TTTAAATCCA CCTCTGACAA TTGTGCGGAA GACATTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTACCA TCAGTAATGA CTTGTGTGAA CTATCTTAAA 
TTGCCGGACT ACTCGAGCAT TGACATAATG CGGGACAAAC TCTTGATAGC AGCAAGAGAA 
GGCCAACAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030248922.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104782.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X17, mRNA.

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CloneID	OMu85011
Clone ID Related Accession (Same CDS sequence)	XM_030248922.1
Accession Version	XM_030248922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AAGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA TCCCTTAGCC 
AATTCTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTAATG 
AAAGAAGATC CGGAACTGGC TAAGTCTTTC ATTAAGACCT TGTTTGGTGT TCTCTATGAA 
GTGTACAGCT CCTCAGCAGG CCCTGCGGTC AGACACAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCTGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCAATGCT ATCCAGCCAA GACCTGAAGA TAGTAGTTGG AGCTCTTCAG 
ATGGCAGAAA TCCTAATGCA AAAGTTACCT GATATTTTTA GTGTTTACTT CAGGAGAGAA 
GGTGTGATGC ATCAAGTCAA ACATTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCCCCC 
AAGGCATGTA CAAATGGGTC AGGATCCCTG GGATCTACAA CACCAGCCAG CAGTGGGACT 
GCAACGGCAG CAACCAACGC CTCTGCTGAT CTGGGGTCAC CTAGCTTGCA GCATAGCAGA 
GATGATTCTT TGGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTCAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA TATTCCCCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCCTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA TATCGAACCA 
GCACGAACTG CAGGAGTTAG TGGTCTTGCC AGGGCAGCCT CAAAAGACAC CATATCCAAT 
AATAGAGAAA AAATTAAAGG CTGGATTAAG GAGCAGGCAC ATAAATTTGT TGAGCGTTAT 
TTTAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCCTTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCT TCATTTGAAA TCCAACATAG TGGGTTTGTG 
AAACAACTGT TGCTGTATTT GACATCTAAG AATGAAAAGG ATGCTGTCGG CAGAGAGATC 
CGGTTAAAAC GTTTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT 
GGAAGAGTAG AACCAGTGGG CCATGCACCT TTGCTGGCAT TGGTTCACAA GATGAACAAC 
TGTCTCAGCC AAATGGAGCA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGGAACGGA 
GCTGGAGGAA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG ATATGGAAGA 
GTAAGAGAAG ATGATGAAGA TAGTGATGAT GATGGGTCAG ATGAAGAAAT AGATGAGTCT 
TTGGCTGCTC AATTCTTAAA TTCGGGAAAT GTAAGGCACA GGCTACAGTT TTATATTGGA 
GAACATTTAC TACCATATAA CATGACCGTG TATCAGGCAG TGCGGCAATT CAGTGTACAG 
GCTGAAGATG AAAGAGAATC TACTGATGAT GAGAGCAATC CTCTGGGAAG AGCTGGAATT 
TGGACAAAGA CTCATACAAT ATGGTACAAA CCTGTGAGGG AGGATGAAGA AAGTACTAAA 
GATTGTGTTG GTGGCAAAAG GGGGAGAGCC CAAACAGCTC CAACAAAAAC TTCCCCCAGA 
AATGCAAAGA AGCATGATGA GTTATGGCAT GATGGAGTGT GCCCGTCAGT AGCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATCA CCTTTGAAGA CCCATCCTTA 
GATGTAATAC TTCTTTTAAG AGTTTTACAT GCCATCAGTC GATATTGGTA TTACTTGTAT 
GATAATGCGA TGTGCAAGGA GATTATTCCA ACTAGTGAAT TTATTAACAG TAAATTAACA 
GCAAAAGCAA ATAGGCAGCT TCAAGACCCT CTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTCACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGACAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGGGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAC AGCAGAGTTG CGCCTAGATT GGATAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTACTG AAACAAGCTG AGTCTGTGAT GCAGGACCTT 
GGCAGCTCTC GGGCCATGTT AGAAATCCAA TATGAAAATG AGGTTGGTAC AGGTCTGGGG 
CCAACACTGG AGTTTTATGC ACTTGTTTCT CAGGAACTAC AGAGAGCTGA CTTGTGTCTC 
TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAC 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCTAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCTCTTGGC CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG 
GAAACTTCAT TGACATCTCA TGATTTATTT GACATTGATC CTGTTGTAGC CAGATCAGTA 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTGG AGCAGGATAA ATCCCAGACC 
AAAGAGAGTC TACAATATGC ATTGGAAACT CTTACGATGA ATGGCTGTTC AGTGGAAGAT 
CTGGGATTGG ATTTTACGCT GCCTGGGTTT CCCAACATTG AATTGAAGAA AGGAGGGAAG 
GATATTCCAG TTACTATCCA CAATTTGGAA GAGTACCTGA GATTGGTGAT ATTCTGGGCA 
CTAAATGAAG GTGTTTGTCG ACAGTTTGAC TCATTCAGAG ATGGATTTGA ATCGGTCTTC 
CCGCTCTGCC ATCTTCAGTA CTTCTACCCA GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACATTG ATGGAATGCT GCAGGCCTGA TCATGGCTAT 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TGTTTCTCCA GTTTGTGACT GGTAGCCCAC GATTGCCAGT TGGAGGATTT 
CGGAGTTTAA ATCCACCTCT GACAATTGTG CGGAAGACAT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TACCATCAGT AATGACTTGT GTGAACTATC TTAAATTGCC GGACTACTCG 
AGCATTGACA TAATGCGGGA CAAACTCTTG ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104782.1
CDS	363..6554
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030248922.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248922.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AAGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA TCCCTTAGCC 
AATTCTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTAATG 
AAAGAAGATC CGGAACTGGC TAAGTCTTTC ATTAAGACCT TGTTTGGTGT TCTCTATGAA 
GTGTACAGCT CCTCAGCAGG CCCTGCGGTC AGACACAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCTGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCAATGCT ATCCAGCCAA GACCTGAAGA TAGTAGTTGG AGCTCTTCAG 
ATGGCAGAAA TCCTAATGCA AAAGTTACCT GATATTTTTA GTGTTTACTT CAGGAGAGAA 
GGTGTGATGC ATCAAGTCAA ACATTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCCCCC 
AAGGCATGTA CAAATGGGTC AGGATCCCTG GGATCTACAA CACCAGCCAG CAGTGGGACT 
GCAACGGCAG CAACCAACGC CTCTGCTGAT CTGGGGTCAC CTAGCTTGCA GCATAGCAGA 
GATGATTCTT TGGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTCAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA TATTCCCCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCCTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA TATCGAACCA 
GCACGAACTG CAGGAGTTAG TGGTCTTGCC AGGGCAGCCT CAAAAGACAC CATATCCAAT 
AATAGAGAAA AAATTAAAGG CTGGATTAAG GAGCAGGCAC ATAAATTTGT TGAGCGTTAT 
TTTAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCCTTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCT TCATTTGAAA TCCAACATAG TGGGTTTGTG 
AAACAACTGT TGCTGTATTT GACATCTAAG AATGAAAAGG ATGCTGTCGG CAGAGAGATC 
CGGTTAAAAC GTTTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT 
GGAAGAGTAG AACCAGTGGG CCATGCACCT TTGCTGGCAT TGGTTCACAA GATGAACAAC 
TGTCTCAGCC AAATGGAGCA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGGAACGGA 
GCTGGAGGAA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG ATATGGAAGA 
GTAAGAGAAG ATGATGAAGA TAGTGATGAT GATGGGTCAG ATGAAGAAAT AGATGAGTCT 
TTGGCTGCTC AATTCTTAAA TTCGGGAAAT GTAAGGCACA GGCTACAGTT TTATATTGGA 
GAACATTTAC TACCATATAA CATGACCGTG TATCAGGCAG TGCGGCAATT CAGTGTACAG 
GCTGAAGATG AAAGAGAATC TACTGATGAT GAGAGCAATC CTCTGGGAAG AGCTGGAATT 
TGGACAAAGA CTCATACAAT ATGGTACAAA CCTGTGAGGG AGGATGAAGA AAGTACTAAA 
GATTGTGTTG GTGGCAAAAG GGGGAGAGCC CAAACAGCTC CAACAAAAAC TTCCCCCAGA 
AATGCAAAGA AGCATGATGA GTTATGGCAT GATGGAGTGT GCCCGTCAGT AGCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATCA CCTTTGAAGA CCCATCCTTA 
GATGTAATAC TTCTTTTAAG AGTTTTACAT GCCATCAGTC GATATTGGTA TTACTTGTAT 
GATAATGCGA TGTGCAAGGA GATTATTCCA ACTAGTGAAT TTATTAACAG TAAATTAACA 
GCAAAAGCAA ATAGGCAGCT TCAAGACCCT CTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTCACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGACAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGGGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAC AGCAGAGTTG CGCCTAGATT GGATAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTACTG AAACAAGCTG AGTCTGTGAT GCAGGACCTT 
GGCAGCTCTC GGGCCATGTT AGAAATCCAA TATGAAAATG AGGTTGGTAC AGGTCTGGGG 
CCAACACTGG AGTTTTATGC ACTTGTTTCT CAGGAACTAC AGAGAGCTGA CTTGTGTCTC 
TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAC 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCTAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCTCTTGGC CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG 
GAAACTTCAT TGACATCTCA TGATTTATTT GACATTGATC CTGTTGTAGC CAGATCAGTA 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTGG AGCAGGATAA ATCCCAGACC 
AAAGAGAGTC TACAATATGC ATTGGAAACT CTTACGATGA ATGGCTGTTC AGTGGAAGAT 
CTGGGATTGG ATTTTACGCT GCCTGGGTTT CCCAACATTG AATTGAAGAA AGGAGGGAAG 
GATATTCCAG TTACTATCCA CAATTTGGAA GAGTACCTGA GATTGGTGAT ATTCTGGGCA 
CTAAATGAAG GTGTTTGTCG ACAGTTTGAC TCATTCAGAG ATGGATTTGA ATCGGTCTTC 
CCGCTCTGCC ATCTTCAGTA CTTCTACCCA GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACATTG ATGGAATGCT GCAGGCCTGA TCATGGCTAT 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TGTTTCTCCA GTTTGTGACT GGTAGCCCAC GATTGCCAGT TGGAGGATTT 
CGGAGTTTAA ATCCACCTCT GACAATTGTG CGGAAGACAT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TACCATCAGT AATGACTTGT GTGAACTATC TTAAATTGCC GGACTACTCG 
AGCATTGACA TAATGCGGGA CAAACTCTTG ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030248942.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104802.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X18, mRNA.

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CloneID	OMu85012
Clone ID Related Accession (Same CDS sequence)	XM_030248942.1
Accession Version	XM_030248942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAAG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAATCC CTTAGCCAAT 
TCTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTAATGAAA 
GAAGATCCGG AACTGGCTAA GTCTTTCATT AAGACCTTGT TTGGTGTTCT CTATGAAGTG 
TACAGCTCCT CAGCAGGCCC TGCGGTCAGA CACAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CTGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CAATGCTATC CAGCCAAGAC CTGAAGATAG TAGTTGGAGC TCTTCAGATG 
GCAGAAATCC TAATGCAAAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG GAGAGAAGGT 
GTGATGCATC AAGTCAAACA TTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCCCCCAAG 
GCATGTACAA ATGGGTCAGG ATCCCTGGGA TCTACAACAC CAGCCAGCAG TGGGACTGCA 
ACGGCAGCAA CCAACGCCTC TGCTGATCTG GGGTCACCTA GCTTGCAGCA TAGCAGAGAT 
GATTCTTTGG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTCAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAATAT TCCCCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCCTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAATAT CGAACCAGCA 
CGAACTGCAG GAGTTAGTGG TCTTGCCAGG GCAGCCTCAA AAGACACCAT ATCCAATAAT 
AGAGAAAAAA TTAAAGGCTG GATTAAGGAG CAGGCACATA AATTTGTTGA GCGTTATTTT 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCCTTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCTTCA TTTGAAATCC AACATAGTGG GTTTGTGAAA 
CAACTGTTGC TGTATTTGAC ATCTAAGAAT GAAAAGGATG CTGTCGGCAG AGAGATCCGG 
TTAAAACGTT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCTGTTGGA 
AGAGTAGAAC CAGTGGGCCA TGCACCTTTG CTGGCATTGG TTCACAAGAT GAACAACTGT 
CTCAGCCAAA TGGAGCAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG GAACGGAGCT 
GGAGGAAGAG GATCACAAGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGTCAGTTA 
CAAAGACACC CAGACTGTGC AAATGTGAAG CAGTGGAAAG GCGGGCCTGT CAAGATTGAT 
CCCCTGGCTC TGGTGCAAGC TATTGAGAGA TACCTTGTGG TTAGAGGATA TGGAAGAGTA 
AGAGAAGATG ATGAAGATAG TGATGATGAT GGGTCAGATG AAGAAATAGA TGAGTCTTTG 
GCTGCTCAAT TCTTAAATTC GGGAAATGTA AGGCACAGGC TACAGTTTTA TATTGGAGAA 
CATTTACTAC CATATAACAT GACCGTGTAT CAGGCAGTGC GGCAATTCAG TGTACAGGCT 
GAAGATGAAA GAGAATCTAC TGATGATGAG AGCAATCCTC TGGGAAGAGC TGGAATTTGG 
ACAAAGACTC ATACAATATG GTACAAACCT GTGAGGGAGG ATGAAGAAAG TACTAAAGAT 
TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA ACAGCTCCAA CAAAAACTTC CCCCAGAAAT 
GCAAAGAAGC ATGATGAGTT ATGGCATGAT GGAGTGTGCC CGTCAGTAGC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATCACCT TTGAAGACCC ATCCTTAGAT 
GTAATACTTC TTTTAAGAGT TTTACATGCC ATCAGTCGAT ATTGGTATTA CTTGTATGAT 
AATGCGATGT GCAAGGAGAT TATTCCAACT AGTGAATTTA TTAACAGTAA ATTAACAGCA 
AAAGCAAATA GGCAGCTTCA AGACCCTCTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTCACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG ACAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGG GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGACAGC AGAGTTGCGC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTACTGAAA CAAGCTGAGT CTGTGATGCA GGACCTTGGC 
AGCTCTCGGG CCATGTTAGA AATCCAATAT GAAAATGAGG TTGGTACAGG TCTGGGGCCA 
ACACTGGAGT TTTATGCACT TGTTTCTCAG GAACTACAGA GAGCTGACTT GTGTCTCTGG 
AGGGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCACATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCTAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC TCTTGGCCTG CCCTTTTATA AGTGGATGCT GCGGCAGGAA 
ACTTCATTGA CATCTCATGA TTTATTTGAC ATTGATCCTG TTGTAGCCAG ATCAGTATAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTGGAGC AGGATAAATC CCAGACCAAA 
GAGAGTCTAC AATATGCATT GGAAACTCTT ACGATGAATG GCTGTTCAGT GGAAGATCTG 
GGATTGGATT TTACGCTGCC TGGGTTTCCC AACATTGAAT TGAAGAAAGG AGGGAAGGAT 
ATTCCAGTTA CTATCCACAA TTTGGAAGAG TACCTGAGAT TGGTGATATT CTGGGCACTA 
AATGAAGGTG TTTGTCGACA GTTTGACTCA TTCAGAGATG GATTTGAATC GGTCTTCCCG 
CTCTGCCATC TTCAGTACTT CTACCCAGAA GAGCTGGACC AGCTCCTGTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACATTGATG GAATGCTGCA GGCCTGATCA TGGCTATACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTGT TTCTCCAGTT TGTGACTGGT AGCCCACGAT TGCCAGTTGG AGGATTTCGG 
AGTTTAAATC CACCTCTGAC AATTGTGCGG AAGACATTTG AATCAACAGA AAACCCAGAT 
GACTTCTTAC CATCAGTAAT GACTTGTGTG AACTATCTTA AATTGCCGGA CTACTCGAGC 
ATTGACATAA TGCGGGACAA ACTCTTGATA GCAGCAAGAG AAGGCCAACA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104802.1
CDS	363..6551
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030248942.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248942.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAAG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAATCC CTTAGCCAAT 
TCTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTAATGAAA 
GAAGATCCGG AACTGGCTAA GTCTTTCATT AAGACCTTGT TTGGTGTTCT CTATGAAGTG 
TACAGCTCCT CAGCAGGCCC TGCGGTCAGA CACAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CTGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CAATGCTATC CAGCCAAGAC CTGAAGATAG TAGTTGGAGC TCTTCAGATG 
GCAGAAATCC TAATGCAAAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG GAGAGAAGGT 
GTGATGCATC AAGTCAAACA TTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCCCCCAAG 
GCATGTACAA ATGGGTCAGG ATCCCTGGGA TCTACAACAC CAGCCAGCAG TGGGACTGCA 
ACGGCAGCAA CCAACGCCTC TGCTGATCTG GGGTCACCTA GCTTGCAGCA TAGCAGAGAT 
GATTCTTTGG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTCAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAATAT TCCCCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCCTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAATAT CGAACCAGCA 
CGAACTGCAG GAGTTAGTGG TCTTGCCAGG GCAGCCTCAA AAGACACCAT ATCCAATAAT 
AGAGAAAAAA TTAAAGGCTG GATTAAGGAG CAGGCACATA AATTTGTTGA GCGTTATTTT 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCCTTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCTTCA TTTGAAATCC AACATAGTGG GTTTGTGAAA 
CAACTGTTGC TGTATTTGAC ATCTAAGAAT GAAAAGGATG CTGTCGGCAG AGAGATCCGG 
TTAAAACGTT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCTGTTGGA 
AGAGTAGAAC CAGTGGGCCA TGCACCTTTG CTGGCATTGG TTCACAAGAT GAACAACTGT 
CTCAGCCAAA TGGAGCAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG GAACGGAGCT 
GGAGGAAGAG GATCACAAGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGTCAGTTA 
CAAAGACACC CAGACTGTGC AAATGTGAAG CAGTGGAAAG GCGGGCCTGT CAAGATTGAT 
CCCCTGGCTC TGGTGCAAGC TATTGAGAGA TACCTTGTGG TTAGAGGATA TGGAAGAGTA 
AGAGAAGATG ATGAAGATAG TGATGATGAT GGGTCAGATG AAGAAATAGA TGAGTCTTTG 
GCTGCTCAAT TCTTAAATTC GGGAAATGTA AGGCACAGGC TACAGTTTTA TATTGGAGAA 
CATTTACTAC CATATAACAT GACCGTGTAT CAGGCAGTGC GGCAATTCAG TGTACAGGCT 
GAAGATGAAA GAGAATCTAC TGATGATGAG AGCAATCCTC TGGGAAGAGC TGGAATTTGG 
ACAAAGACTC ATACAATATG GTACAAACCT GTGAGGGAGG ATGAAGAAAG TACTAAAGAT 
TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA ACAGCTCCAA CAAAAACTTC CCCCAGAAAT 
GCAAAGAAGC ATGATGAGTT ATGGCATGAT GGAGTGTGCC CGTCAGTAGC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATCACCT TTGAAGACCC ATCCTTAGAT 
GTAATACTTC TTTTAAGAGT TTTACATGCC ATCAGTCGAT ATTGGTATTA CTTGTATGAT 
AATGCGATGT GCAAGGAGAT TATTCCAACT AGTGAATTTA TTAACAGTAA ATTAACAGCA 
AAAGCAAATA GGCAGCTTCA AGACCCTCTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTCACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG ACAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGG GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGACAGC AGAGTTGCGC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTACTGAAA CAAGCTGAGT CTGTGATGCA GGACCTTGGC 
AGCTCTCGGG CCATGTTAGA AATCCAATAT GAAAATGAGG TTGGTACAGG TCTGGGGCCA 
ACACTGGAGT TTTATGCACT TGTTTCTCAG GAACTACAGA GAGCTGACTT GTGTCTCTGG 
AGGGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCACATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCTAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC TCTTGGCCTG CCCTTTTATA AGTGGATGCT GCGGCAGGAA 
ACTTCATTGA CATCTCATGA TTTATTTGAC ATTGATCCTG TTGTAGCCAG ATCAGTATAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTGGAGC AGGATAAATC CCAGACCAAA 
GAGAGTCTAC AATATGCATT GGAAACTCTT ACGATGAATG GCTGTTCAGT GGAAGATCTG 
GGATTGGATT TTACGCTGCC TGGGTTTCCC AACATTGAAT TGAAGAAAGG AGGGAAGGAT 
ATTCCAGTTA CTATCCACAA TTTGGAAGAG TACCTGAGAT TGGTGATATT CTGGGCACTA 
AATGAAGGTG TTTGTCGACA GTTTGACTCA TTCAGAGATG GATTTGAATC GGTCTTCCCG 
CTCTGCCATC TTCAGTACTT CTACCCAGAA GAGCTGGACC AGCTCCTGTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACATTGATG GAATGCTGCA GGCCTGATCA TGGCTATACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTGT TTCTCCAGTT TGTGACTGGT AGCCCACGAT TGCCAGTTGG AGGATTTCGG 
AGTTTAAATC CACCTCTGAC AATTGTGCGG AAGACATTTG AATCAACAGA AAACCCAGAT 
GACTTCTTAC CATCAGTAAT GACTTGTGTG AACTATCTTA AATTGCCGGA CTACTCGAGC 
ATTGACATAA TGCGGGACAA ACTCTTGATA GCAGCAAGAG AAGGCCAACA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030248998.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104858.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X23, mRNA.

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CloneID	OMu85013
Clone ID Related Accession (Same CDS sequence)	XM_030248998.1
Accession Version	XM_030248998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAAGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAATCCC TTAGCCAATT CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTAATGAAAG AAGATCCGGA ACTGGCTAAG 
TCTTTCATTA AGACCTTGTT TGGTGTTCTC TATGAAGTGT ACAGCTCCTC AGCAGGCCCT 
GCGGTCAGAC ACAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC TGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC AATGCTATCC 
AGCCAAGACC TGAAGATAGT AGTTGGAGCT CTTCAGATGG CAGAAATCCT AATGCAAAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGG AGAGAAGGTG TGATGCATCA AGTCAAACAT 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCCCCCAAGG CATGTACAAA TGGGTCAGGA 
TCCCTGGGAT CTACAACACC AGCCAGCAGT GGGACTGCAA CGGCAGCAAC CAACGCCTCT 
GCTGATCTGG GGTCACCTAG CTTGCAGCAT AGCAGAGATG ATTCTTTGGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTCAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAATATT CCCCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCCTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAATATC GAACCAGCAC GAACTGCAGG AGTTAGTGGT 
CTTGCCAGGG CAGCCTCAAA AGACACCATA TCCAATAATA GAGAAAAAAT TAAAGGCTGG 
ATTAAGGAGC AGGCACATAA ATTTGTTGAG CGTTATTTTA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CCTTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCTTCAT TTGAAATCCA ACATAGTGGG TTTGTGAAAC AACTGTTGCT GTATTTGACA 
TCTAAGAATG AAAAGGATGC TGTCGGCAGA GAGATCCGGT TAAAACGTTT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCTGTTGGAA GAGTAGAACC AGTGGGCCAT 
GCACCTTTGC TGGCATTGGT TCACAAGATG AACAACTGTC TCAGCCAAAT GGAGCAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGG AACGGAGCTG GAGGAAGCTT TTCTCTCAAC 
AGAGGATCAC AAGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGTCA GTTACAAAGA 
CACCCAGACT GTGCAAATGT GAAGCAGTGG AAAGGCGGGC CTGTCAAGAT TGATCCCCTG 
GCTCTGGTGC AAGCTATTGA GAGATACCTT GTGGTTAGAG GTATTTTCCG ATATGGAAGA 
GTAAGAGAAG ATGATGAAGA TAGTGATGAT GATGGGTCAG ATGAAGAAAT AGATGAGTCT 
TTGGCTGCTC AATTCTTAAA TTCGGGAAAT GTAAGGCACA GGCTACAGTT TTATATTGGA 
GAACATTTAC TACCATATAA CATGACCGTG TATCAGGCAG TGCGGCAATT CAGTGTACAG 
GCTGAAGATG AAAGAGAATC TACTGATGAT GAGAGCAATC CTCTGGGAAG AGCTGGAATT 
TGGACAAAGA CTCATACAAT ATGGTACAAA CCTGTGAGGG AGGATGAAGA AAGTACTAAA 
GATTGTGTTG GTGGCAAAAG GGGGAGAGCC CAAACAGCTC CAACAAAAAC TTCCCCCAGA 
AATGCAAAGA AGCATGATGA GTTATGGCAT GATGGAGTGT GCCCGTCAGT AGCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATCA CCTTTGAAGA CCCATCCTTA 
GATGTAATAC TTCTTTTAAG AGTTTTACAT GCCATCAGTC GATATTGGTA TTACTTGTAT 
GATAATGCGA TGTGCAAGGA GATTATTCCA ACTAGTGAAT TTATTAACAG TAAATTAACA 
GCAAAAGCAA ATAGGCAGCT TCAAGACCCT CTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTCACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGACAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGGGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAC AGCAGAGTTG CGCCTAGATT GGATAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTACTG AAACAAGCTG AGTCTGTGAT GCAGGACCTT 
GGCAGCTCTC GGGCCATGTT AGAAATCCAA TATGAAAATG AGGTTGGTAC AGGTCTGGGG 
CCAACACTGG AGTTTTATGC ACTTGTTTCT CAGGAACTAC AGAGAGCTGA CTTGTGTCTC 
TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAC 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCTAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCTCTTGGC CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG 
GAAACTTCAT TGACATCTCA TGATTTATTT GACATTGATC CTGTTGTAGC CAGATCAGTA 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTGG AGCAGGATAA ATCCCAGACC 
AAAGAGAGTC TACAATATGC ATTGGAAACT CTTACGATGA ATGGCTGTTC AGTGGAAGAT 
CTGGGATTGG ATTTTACGCT GCCTGGGTTT CCCAACATTG AATTGAAGAA AGGAGGGAAG 
GATATTCCAG TTACTATCCA CAATTTGGAA GAGTACCTGA GATTGGTGAT ATTCTGGGCA 
CTAAATGAAG GTGTTTGTCG ACAGTTTGAC TCATTCAGAG ATGGATTTGA ATCGGTCTTC 
CCGCTCTGCC ATCTTCAGTA CTTCTACCCA GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACATTG ATGGAATGCT GCAGGCCTGA TCATGGCTAT 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TGTTTCTCCA GTTTGTGACT GGTAGCCCAC GATTGCCAGT TGGAGGATTT 
CGGAGTTTAA ATCCACCTCT GACAATTGTG CGGAAGACAT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TACCATCAGT AATGACTTGT GTGAACTATC TTAAATTGCC GGACTACTCG 
AGCATTGACA TAATGCGGGA CAAACTCTTG ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104858.1
CDS	363..6494
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030248998.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248998.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAAGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAATCCC TTAGCCAATT CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTAATGAAAG AAGATCCGGA ACTGGCTAAG 
TCTTTCATTA AGACCTTGTT TGGTGTTCTC TATGAAGTGT ACAGCTCCTC AGCAGGCCCT 
GCGGTCAGAC ACAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC TGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC AATGCTATCC 
AGCCAAGACC TGAAGATAGT AGTTGGAGCT CTTCAGATGG CAGAAATCCT AATGCAAAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGG AGAGAAGGTG TGATGCATCA AGTCAAACAT 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCCCCCAAGG CATGTACAAA TGGGTCAGGA 
TCCCTGGGAT CTACAACACC AGCCAGCAGT GGGACTGCAA CGGCAGCAAC CAACGCCTCT 
GCTGATCTGG GGTCACCTAG CTTGCAGCAT AGCAGAGATG ATTCTTTGGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTCAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAATATT CCCCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCCTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAATATC GAACCAGCAC GAACTGCAGG AGTTAGTGGT 
CTTGCCAGGG CAGCCTCAAA AGACACCATA TCCAATAATA GAGAAAAAAT TAAAGGCTGG 
ATTAAGGAGC AGGCACATAA ATTTGTTGAG CGTTATTTTA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CCTTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCTTCAT TTGAAATCCA ACATAGTGGG TTTGTGAAAC AACTGTTGCT GTATTTGACA 
TCTAAGAATG AAAAGGATGC TGTCGGCAGA GAGATCCGGT TAAAACGTTT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCTGTTGGAA GAGTAGAACC AGTGGGCCAT 
GCACCTTTGC TGGCATTGGT TCACAAGATG AACAACTGTC TCAGCCAAAT GGAGCAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGG AACGGAGCTG GAGGAAGCTT TTCTCTCAAC 
AGAGGATCAC AAGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGTCA GTTACAAAGA 
CACCCAGACT GTGCAAATGT GAAGCAGTGG AAAGGCGGGC CTGTCAAGAT TGATCCCCTG 
GCTCTGGTGC AAGCTATTGA GAGATACCTT GTGGTTAGAG GTATTTTCCG ATATGGAAGA 
GTAAGAGAAG ATGATGAAGA TAGTGATGAT GATGGGTCAG ATGAAGAAAT AGATGAGTCT 
TTGGCTGCTC AATTCTTAAA TTCGGGAAAT GTAAGGCACA GGCTACAGTT TTATATTGGA 
GAACATTTAC TACCATATAA CATGACCGTG TATCAGGCAG TGCGGCAATT CAGTGTACAG 
GCTGAAGATG AAAGAGAATC TACTGATGAT GAGAGCAATC CTCTGGGAAG AGCTGGAATT 
TGGACAAAGA CTCATACAAT ATGGTACAAA CCTGTGAGGG AGGATGAAGA AAGTACTAAA 
GATTGTGTTG GTGGCAAAAG GGGGAGAGCC CAAACAGCTC CAACAAAAAC TTCCCCCAGA 
AATGCAAAGA AGCATGATGA GTTATGGCAT GATGGAGTGT GCCCGTCAGT AGCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATCA CCTTTGAAGA CCCATCCTTA 
GATGTAATAC TTCTTTTAAG AGTTTTACAT GCCATCAGTC GATATTGGTA TTACTTGTAT 
GATAATGCGA TGTGCAAGGA GATTATTCCA ACTAGTGAAT TTATTAACAG TAAATTAACA 
GCAAAAGCAA ATAGGCAGCT TCAAGACCCT CTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTCACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGACAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGGGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAC AGCAGAGTTG CGCCTAGATT GGATAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTACTG AAACAAGCTG AGTCTGTGAT GCAGGACCTT 
GGCAGCTCTC GGGCCATGTT AGAAATCCAA TATGAAAATG AGGTTGGTAC AGGTCTGGGG 
CCAACACTGG AGTTTTATGC ACTTGTTTCT CAGGAACTAC AGAGAGCTGA CTTGTGTCTC 
TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAC 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCTAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCTCTTGGC CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG 
GAAACTTCAT TGACATCTCA TGATTTATTT GACATTGATC CTGTTGTAGC CAGATCAGTA 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTGG AGCAGGATAA ATCCCAGACC 
AAAGAGAGTC TACAATATGC ATTGGAAACT CTTACGATGA ATGGCTGTTC AGTGGAAGAT 
CTGGGATTGG ATTTTACGCT GCCTGGGTTT CCCAACATTG AATTGAAGAA AGGAGGGAAG 
GATATTCCAG TTACTATCCA CAATTTGGAA GAGTACCTGA GATTGGTGAT ATTCTGGGCA 
CTAAATGAAG GTGTTTGTCG ACAGTTTGAC TCATTCAGAG ATGGATTTGA ATCGGTCTTC 
CCGCTCTGCC ATCTTCAGTA CTTCTACCCA GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACATTG ATGGAATGCT GCAGGCCTGA TCATGGCTAT 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TGTTTCTCCA GTTTGTGACT GGTAGCCCAC GATTGCCAGT TGGAGGATTT 
CGGAGTTTAA ATCCACCTCT GACAATTGTG CGGAAGACAT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TACCATCAGT AATGACTTGT GTGAACTATC TTAAATTGCC GGACTACTCG 
AGCATTGACA TAATGCGGGA CAAACTCTTG ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249012.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104872.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X24, mRNA.

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CloneID	OMu85014
Clone ID Related Accession (Same CDS sequence)	XM_030249012.1
Accession Version	XM_030249012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAC AAATCAATGA AGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAT CCCTTAGCCA ATTCTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTAATGA AAGAAGATCC GGAACTGGCT 
AAGTCTTTCA TTAAGACCTT GTTTGGTGTT CTCTATGAAG TGTACAGCTC CTCAGCAGGC 
CCTGCGGTCA GACACAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCTGATGCT 
GAACTTCTGA AGGATGTTCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCAATGCTA 
TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA GCTCTTCAGA TGGCAGAAAT CCTAATGCAA 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGGAGAGAAG GTGTGATGCA TCAAGTCAAA 
CATTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCCCCCA AGGCATGTAC AAATGGGTCA 
GGATCCCTGG GATCTACAAC ACCAGCCAGC AGTGGGACTG CAACGGCAGC AACCAACGCC 
TCTGCTGATC TGGGGTCACC TAGCTTGCAG CATAGCAGAG ATGATTCTTT GGATCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTCAAG AGAAAACGAC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAAT ATTCCCCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACCACTACTC AGTCACCTAA ATCTTCCTTC CTGGCAAGCT TGAATCCAAA AACATGGGGA 
AGGTTAAGCG CACAGTCCAA CAGCAACAAT ATCGAACCAG CACGAACTGC AGGAGTTAGT 
GGTCTTGCCA GGGCAGCCTC AAAAGACACC ATATCCAATA ATAGAGAAAA AATTAAAGGC 
TGGATTAAGG AGCAGGCACA TAAATTTGTT GAGCGTTATT TTAGTTCTGA GAATATGGAT 
GGAAGCAACC CTGCCTTGAA TGTCCTTCAG AGACTTTGTG CTGCAACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCA 
GATGTTTCTT CATTTGAAAT CCAACATAGT GGGTTTGTGA AACAACTGTT GCTGTATTTG 
ACATCTAAGA ATGAAAAGGA TGCTGTCGGC AGAGAGATCC GGTTAAAACG TTTCCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC 
CATGCACCTT TGCTGGCATT GGTTCACAAG ATGAACAACT GTCTCAGCCA AATGGAGCAA 
TTTCCAGTCA AAGTACATGA TTTCCCTAGT GGGAACGGAG CTGGAGGAAG CTTTTCTCTC 
AACAGAGGAT CACAAGCTTT AAAATTTTTC AACACACATC AGTTAAAATG TCAGTTACAA 
AGACACCCAG ACTGTGCAAA TGTGAAGCAG TGGAAAGGCG GGCCTGTCAA GATTGATCCC 
CTGGCTCTGG TGCAAGCTAT TGAGAGATAC CTTGTGGTTA GAGGATATGG AAGAGTAAGA 
GAAGATGATG AAGATAGTGA TGATGATGGG TCAGATGAAG AAATAGATGA GTCTTTGGCT 
GCTCAATTCT TAAATTCGGG AAATGTAAGG CACAGGCTAC AGTTTTATAT TGGAGAACAT 
TTACTACCAT ATAACATGAC CGTGTATCAG GCAGTGCGGC AATTCAGTGT ACAGGCTGAA 
GATGAAAGAG AATCTACTGA TGATGAGAGC AATCCTCTGG GAAGAGCTGG AATTTGGACA 
AAGACTCATA CAATATGGTA CAAACCTGTG AGGGAGGATG AAGAAAGTAC TAAAGATTGT 
GTTGGTGGCA AAAGGGGGAG AGCCCAAACA GCTCCAACAA AAACTTCCCC CAGAAATGCA 
AAGAAGCATG ATGAGTTATG GCATGATGGA GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA 
GTTTACCTCA TTCCCACACC ACCTGAAAAT ATCACCTTTG AAGACCCATC CTTAGATGTA 
ATACTTCTTT TAAGAGTTTT ACATGCCATC AGTCGATATT GGTATTACTT GTATGATAAT 
GCGATGTGCA AGGAGATTAT TCCAACTAGT GAATTTATTA ACAGTAAATT AACAGCAAAA 
GCAAATAGGC AGCTTCAAGA CCCTCTAGTA ATCATGACAG GAAACATCCC AACATGGCTC 
ACTGAGCTAG GAAAAACCTG CCCATTTTTC TTTCCTTTTG ATACCCGACA AATGCTTTTT 
TATGTAACTG CATTTGATCG GGACCGGGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CTGATTCTCA AGACAGCAGA GTTGCGCCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT ACTGAAACAA GCTGAGTCTG TGATGCAGGA CCTTGGCAGC 
TCTCGGGCCA TGTTAGAAAT CCAATATGAA AATGAGGTTG GTACAGGTCT GGGGCCAACA 
CTGGAGTTTT ATGCACTTGT TTCTCAGGAA CTACAGAGAG CTGACTTGTG TCTCTGGAGG 
GGTGAAGAAG TAACTCTTAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC GCTTCCCTTT GGTAGGACAG CTAAGCCAGC TCACATCGCA 
AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CTAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCTCT TGGCCTGCCC TTTTATAAGT GGATGCTGCG GCAGGAAACT 
TCATTGACAT CTCATGATTT ATTTGACATT GATCCTGTTG TAGCCAGATC AGTATATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTGGAGCAGG ATAAATCCCA GACCAAAGAG 
AGTCTACAAT ATGCATTGGA AACTCTTACG ATGAATGGCT GTTCAGTGGA AGATCTGGGA 
TTGGATTTTA CGCTGCCTGG GTTTCCCAAC ATTGAATTGA AGAAAGGAGG GAAGGATATT 
CCAGTTACTA TCCACAATTT GGAAGAGTAC CTGAGATTGG TGATATTCTG GGCACTAAAT 
GAAGGTGTTT GTCGACAGTT TGACTCATTC AGAGATGGAT TTGAATCGGT CTTCCCGCTC 
TGCCATCTTC AGTACTTCTA CCCAGAAGAG CTGGACCAGC TCCTGTGTGG CAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ATTGATGGAA TGCTGCAGGC CTGATCATGG CTATACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTGTTTC TCCAGTTTGT GACTGGTAGC CCACGATTGC CAGTTGGAGG ATTTCGGAGT 
TTAAATCCAC CTCTGACAAT TGTGCGGAAG ACATTTGAAT CAACAGAAAA CCCAGATGAC 
TTCTTACCAT CAGTAATGAC TTGTGTGAAC TATCTTAAAT TGCCGGACTA CTCGAGCATT 
GACATAATGC GGGACAAACT CTTGATAGCA GCAAGAGAAG GCCAACAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104872.1
CDS	363..6488
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249012.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249012.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAC AAATCAATGA AGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAT CCCTTAGCCA ATTCTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTAATGA AAGAAGATCC GGAACTGGCT 
AAGTCTTTCA TTAAGACCTT GTTTGGTGTT CTCTATGAAG TGTACAGCTC CTCAGCAGGC 
CCTGCGGTCA GACACAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCTGATGCT 
GAACTTCTGA AGGATGTTCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCAATGCTA 
TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA GCTCTTCAGA TGGCAGAAAT CCTAATGCAA 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGGAGAGAAG GTGTGATGCA TCAAGTCAAA 
CATTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCCCCCA AGGCATGTAC AAATGGGTCA 
GGATCCCTGG GATCTACAAC ACCAGCCAGC AGTGGGACTG CAACGGCAGC AACCAACGCC 
TCTGCTGATC TGGGGTCACC TAGCTTGCAG CATAGCAGAG ATGATTCTTT GGATCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTCAAG AGAAAACGAC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAAT ATTCCCCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACCACTACTC AGTCACCTAA ATCTTCCTTC CTGGCAAGCT TGAATCCAAA AACATGGGGA 
AGGTTAAGCG CACAGTCCAA CAGCAACAAT ATCGAACCAG CACGAACTGC AGGAGTTAGT 
GGTCTTGCCA GGGCAGCCTC AAAAGACACC ATATCCAATA ATAGAGAAAA AATTAAAGGC 
TGGATTAAGG AGCAGGCACA TAAATTTGTT GAGCGTTATT TTAGTTCTGA GAATATGGAT 
GGAAGCAACC CTGCCTTGAA TGTCCTTCAG AGACTTTGTG CTGCAACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCA 
GATGTTTCTT CATTTGAAAT CCAACATAGT GGGTTTGTGA AACAACTGTT GCTGTATTTG 
ACATCTAAGA ATGAAAAGGA TGCTGTCGGC AGAGAGATCC GGTTAAAACG TTTCCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC 
CATGCACCTT TGCTGGCATT GGTTCACAAG ATGAACAACT GTCTCAGCCA AATGGAGCAA 
TTTCCAGTCA AAGTACATGA TTTCCCTAGT GGGAACGGAG CTGGAGGAAG CTTTTCTCTC 
AACAGAGGAT CACAAGCTTT AAAATTTTTC AACACACATC AGTTAAAATG TCAGTTACAA 
AGACACCCAG ACTGTGCAAA TGTGAAGCAG TGGAAAGGCG GGCCTGTCAA GATTGATCCC 
CTGGCTCTGG TGCAAGCTAT TGAGAGATAC CTTGTGGTTA GAGGATATGG AAGAGTAAGA 
GAAGATGATG AAGATAGTGA TGATGATGGG TCAGATGAAG AAATAGATGA GTCTTTGGCT 
GCTCAATTCT TAAATTCGGG AAATGTAAGG CACAGGCTAC AGTTTTATAT TGGAGAACAT 
TTACTACCAT ATAACATGAC CGTGTATCAG GCAGTGCGGC AATTCAGTGT ACAGGCTGAA 
GATGAAAGAG AATCTACTGA TGATGAGAGC AATCCTCTGG GAAGAGCTGG AATTTGGACA 
AAGACTCATA CAATATGGTA CAAACCTGTG AGGGAGGATG AAGAAAGTAC TAAAGATTGT 
GTTGGTGGCA AAAGGGGGAG AGCCCAAACA GCTCCAACAA AAACTTCCCC CAGAAATGCA 
AAGAAGCATG ATGAGTTATG GCATGATGGA GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA 
GTTTACCTCA TTCCCACACC ACCTGAAAAT ATCACCTTTG AAGACCCATC CTTAGATGTA 
ATACTTCTTT TAAGAGTTTT ACATGCCATC AGTCGATATT GGTATTACTT GTATGATAAT 
GCGATGTGCA AGGAGATTAT TCCAACTAGT GAATTTATTA ACAGTAAATT AACAGCAAAA 
GCAAATAGGC AGCTTCAAGA CCCTCTAGTA ATCATGACAG GAAACATCCC AACATGGCTC 
ACTGAGCTAG GAAAAACCTG CCCATTTTTC TTTCCTTTTG ATACCCGACA AATGCTTTTT 
TATGTAACTG CATTTGATCG GGACCGGGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CTGATTCTCA AGACAGCAGA GTTGCGCCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT ACTGAAACAA GCTGAGTCTG TGATGCAGGA CCTTGGCAGC 
TCTCGGGCCA TGTTAGAAAT CCAATATGAA AATGAGGTTG GTACAGGTCT GGGGCCAACA 
CTGGAGTTTT ATGCACTTGT TTCTCAGGAA CTACAGAGAG CTGACTTGTG TCTCTGGAGG 
GGTGAAGAAG TAACTCTTAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC GCTTCCCTTT GGTAGGACAG CTAAGCCAGC TCACATCGCA 
AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CTAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCTCT TGGCCTGCCC TTTTATAAGT GGATGCTGCG GCAGGAAACT 
TCATTGACAT CTCATGATTT ATTTGACATT GATCCTGTTG TAGCCAGATC AGTATATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTGGAGCAGG ATAAATCCCA GACCAAAGAG 
AGTCTACAAT ATGCATTGGA AACTCTTACG ATGAATGGCT GTTCAGTGGA AGATCTGGGA 
TTGGATTTTA CGCTGCCTGG GTTTCCCAAC ATTGAATTGA AGAAAGGAGG GAAGGATATT 
CCAGTTACTA TCCACAATTT GGAAGAGTAC CTGAGATTGG TGATATTCTG GGCACTAAAT 
GAAGGTGTTT GTCGACAGTT TGACTCATTC AGAGATGGAT TTGAATCGGT CTTCCCGCTC 
TGCCATCTTC AGTACTTCTA CCCAGAAGAG CTGGACCAGC TCCTGTGTGG CAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ATTGATGGAA TGCTGCAGGC CTGATCATGG CTATACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTGTTTC TCCAGTTTGT GACTGGTAGC CCACGATTGC CAGTTGGAGG ATTTCGGAGT 
TTAAATCCAC CTCTGACAAT TGTGCGGAAG ACATTTGAAT CAACAGAAAA CCCAGATGAC 
TTCTTACCAT CAGTAATGAC TTGTGTGAAC TATCTTAAAT TGCCGGACTA CTCGAGCATT 
GACATAATGC GGGACAAACT CTTGATAGCA GCAAGAGAAG GCCAACAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249060.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104920.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X27, mRNA.

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CloneID	OMu85015
Clone ID Related Accession (Same CDS sequence)	XM_030249060.1
Accession Version	XM_030249060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA GTCCACCAAG 
AAACGCAGTG AGTCCCCACC TGCTGAGCTC CCCAGTTTGA GGAGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA GAGAGGAGCA 
GCTGAGGCCC GGCGGCAAGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GACAGTAAAT 
TCCTCAGCTG CCCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCGG ATGATTCTGA GATGGGACGA 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCTCCTCACC TATTTGGTCC TCTCGGGCCT 
CGGATGTCAC AACTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTTCAAG GACTACAAGC CAGTGATGAA AGTCAACAGC TTCAGGCCGT CATTGAAATG 
TGTCAGTTAT TGGTCATGGG GAATGAGGAG ACACTAGGAG GGTTTCCTGT GAAGAGTGTT 
GTTCCAGCTT TGATAACATT ACTGCAGATG GAACATAATT TTGACATTAT GAATCATGCT 
TGCCGAGCCT TAACATATAT GATGGAAGCT CTCCCCCGGT CATCTGCTGT TGTAGTGGAT 
GCTATCCCTG TTTTCTTAGA AAAGTTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG CGACACAGTA AAGCCATTTT ACAAGCGGGT 
GGTTTGGCAG ATTGCTTGTT GTATCTAGAA TTCTTCAGCA TCAATGCCCA AAGAAATGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACACCAG ATGAGTTTCA TTTTGTGGCA 
GATTCCCTAC CATTGCTTAC CCAAAGGCTA ACTCATCAGG ACAAAAAGTC AGTAGAAAGT 
ACTTGCCTTT GTTTTGCACG TTTAGTGGAC AACTTTCAAC ATGAGGAGAA CTTACTGCAA 
CAGGTTGCTT CCAAAGATCT ACTTACAAAT GTTCAACAAT TGTTGGTAGT GACTCCACCG 
ATTTTAAGTT CTGGGATGTT TATTATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACAT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACACT TCACTTCCTG 
CTATGTGGTG CCTCCAATGG AAGTTGTCAA GAGCAGATTG ACCTTGTTCC ACGAAGTCCT 
CAAGAGCTGT ATGAGCTGAC ATCTTTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATCTTCG CAGTTGATAC CATGTTGAAA AAGGGAAATG CACAGAACAC AGATGGTGCA 
ATATGGCAGT GGCGGGATGA CCGAGGCCTC TGGCATCCCT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AAGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA TCCCTTAGCC AATTCTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTAATG AAAGAAGATC CGGAACTGGC TAAGTCTTTC 
ATTAAGACCT TGTTTGGTGT TCTCTATGAA GTGTACAGCT CCTCAGCAGG CCCTGCGGTC 
AGACACAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCTGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCAATGCT ATCCAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCTCTTCAG ATGGCAGAAA TCCTAATGCA AAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGGAGAGAA GGTGTGATGC ATCAAGTCAA ACATTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCCCCC AAGGCATGTA CAAATGGGTC AGGATCCCTG 
GGATCTACAA CACCAGCCAG CAGTGGGACT GCAACGGCAG CAACCAACGC CTCTGCTGAT 
CTGGGGTCAC CTAGCTTGCA GCATAGCAGA GATGATTCTT TGGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTCAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA 
TATTCCCCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCCTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA TATCGAACCA GCACGAACTG CAGGAGTTAG TGGTCTTGCC 
AGGGCAGCCT CAAAAGACAC CATATCCAAT AATAGAGAAA AAATTAAAGG CTGGATTAAG 
GAGCAGGCAC ATAAATTTGT TGAGCGTTAT TTTAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCCTTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCT 
TCATTTGAAA TCCAACATAG TGGGTTTGTG AAACAACTGT TGCTGTATTT GACATCTAAG 
AATGAAAAGG ATGCTGTCGG CAGAGAGATC CGGTTAAAAC GTTTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT GGAAGAGTAG AACCAGTGGG CCATGCACCT 
TTGCTGGCAT TGGTTCACAA GATGAACAAC TGTCTCAGCC AAATGGAGCA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGGAACGGA GCTGGAGGAA GAGGATCACA AGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGTCAG TTACAAAGAC ACCCAGACTG TGCAAATGTG 
AAGCAGTGGA AAGGCGGGCC TGTCAAGATT GATCCCCTGG CTCTGGTGCA AGCTATTGAG 
AGATACCTTG TGGTTAGAGG ATATGGAAGA GTAAGAGAAG ATGATGAAGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAGTCT TTGGCTGCTC AATTCTTAAA TTCGGGAAAT 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTAC TACCATATAA CATGACCGTG 
TATCAGGCAG TGCGGCAATT CAGTGTACAG GCTGAAGATG AAAGAGAATC TACTGATGAT 
GAGAGCAATC CTCTGGGAAG AGCTGGAATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGGG AGGATGAAGA AAGTACTAAA GATTGTGTTG GTGGCAAAAG GGGGAGAGCC 
CAAACAGCTC CAACAAAAAC TTCCCCCAGA AATGCAAAGA AGCATGATGA GTTATGGCAT 
GATGGAGTGT GCCCGTCAGT AGCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATCA CCTTTGAAGA CCCATCCTTA GATGTAATAC TTCTTTTAAG AGTTTTACAT 
GCCATCAGTC GATATTGGTA TTACTTGTAT GATAATGCGA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAATTAACA GCAAAAGCAA ATAGGCAGCT TCAAGACCCT 
CTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTCACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGACAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGGGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAC 
AGCAGAGTTG CGCCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTACTG 
AAACAAGCTG AGTCTGTGAT GCAGGACCTT GGCAGCTCTC GGGCCATGTT AGAAATCCAA 
TATGAAAATG AGGTTGGTAC AGGTCTGGGG CCAACACTGG AGTTTTATGC ACTTGTTTCT 
CAGGAACTAC AGAGAGCTGA CTTGTGTCTC TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAC ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCTAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCTCTTGGC 
CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG GAAACTTCAT TGACATCTCA TGATTTATTT 
GACATTGATC CTGTTGTAGC CAGATCAGTA TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTGG AGCAGGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTGGAAACT 
CTTACGATGA ATGGCTGTTC AGTGGAAGAT CTGGGATTGG ATTTTACGCT GCCTGGGTTT 
CCCAACATTG AATTGAAGAA AGGAGGGAAG GATATTCCAG TTACTATCCA CAATTTGGAA 
GAGTACCTGA GATTGGTGAT ATTCTGGGCA CTAAATGAAG GTGTTTGTCG ACAGTTTGAC 
TCATTCAGAG ATGGATTTGA ATCGGTCTTC CCGCTCTGCC ATCTTCAGTA CTTCTACCCA 
GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACATTG 
ATGGAATGCT GCAGGCCTGA TCATGGCTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACT 
GGTAGCCCAC GATTGCCAGT TGGAGGATTT CGGAGTTTAA ATCCACCTCT GACAATTGTG 
CGGAAGACAT TTGAATCAAC AGAAAACCCA GATGACTTCT TACCATCAGT AATGACTTGT 
GTGAACTATC TTAAATTGCC GGACTACTCG AGCATTGACA TAATGCGGGA CAAACTCTTG 
ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104920.1
CDS	363..6464
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249060.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249060.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA GTCCACCAAG 
AAACGCAGTG AGTCCCCACC TGCTGAGCTC CCCAGTTTGA GGAGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA GAGAGGAGCA 
GCTGAGGCCC GGCGGCAAGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GACAGTAAAT 
TCCTCAGCTG CCCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCGG ATGATTCTGA GATGGGACGA 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCTCCTCACC TATTTGGTCC TCTCGGGCCT 
CGGATGTCAC AACTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTTCAAG GACTACAAGC CAGTGATGAA AGTCAACAGC TTCAGGCCGT CATTGAAATG 
TGTCAGTTAT TGGTCATGGG GAATGAGGAG ACACTAGGAG GGTTTCCTGT GAAGAGTGTT 
GTTCCAGCTT TGATAACATT ACTGCAGATG GAACATAATT TTGACATTAT GAATCATGCT 
TGCCGAGCCT TAACATATAT GATGGAAGCT CTCCCCCGGT CATCTGCTGT TGTAGTGGAT 
GCTATCCCTG TTTTCTTAGA AAAGTTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG CGACACAGTA AAGCCATTTT ACAAGCGGGT 
GGTTTGGCAG ATTGCTTGTT GTATCTAGAA TTCTTCAGCA TCAATGCCCA AAGAAATGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACACCAG ATGAGTTTCA TTTTGTGGCA 
GATTCCCTAC CATTGCTTAC CCAAAGGCTA ACTCATCAGG ACAAAAAGTC AGTAGAAAGT 
ACTTGCCTTT GTTTTGCACG TTTAGTGGAC AACTTTCAAC ATGAGGAGAA CTTACTGCAA 
CAGGTTGCTT CCAAAGATCT ACTTACAAAT GTTCAACAAT TGTTGGTAGT GACTCCACCG 
ATTTTAAGTT CTGGGATGTT TATTATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACAT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACACT TCACTTCCTG 
CTATGTGGTG CCTCCAATGG AAGTTGTCAA GAGCAGATTG ACCTTGTTCC ACGAAGTCCT 
CAAGAGCTGT ATGAGCTGAC ATCTTTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATCTTCG CAGTTGATAC CATGTTGAAA AAGGGAAATG CACAGAACAC AGATGGTGCA 
ATATGGCAGT GGCGGGATGA CCGAGGCCTC TGGCATCCCT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AAGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA TCCCTTAGCC AATTCTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTAATG AAAGAAGATC CGGAACTGGC TAAGTCTTTC 
ATTAAGACCT TGTTTGGTGT TCTCTATGAA GTGTACAGCT CCTCAGCAGG CCCTGCGGTC 
AGACACAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCTGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCAATGCT ATCCAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCTCTTCAG ATGGCAGAAA TCCTAATGCA AAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGGAGAGAA GGTGTGATGC ATCAAGTCAA ACATTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCCCCC AAGGCATGTA CAAATGGGTC AGGATCCCTG 
GGATCTACAA CACCAGCCAG CAGTGGGACT GCAACGGCAG CAACCAACGC CTCTGCTGAT 
CTGGGGTCAC CTAGCTTGCA GCATAGCAGA GATGATTCTT TGGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTCAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA 
TATTCCCCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCCTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA TATCGAACCA GCACGAACTG CAGGAGTTAG TGGTCTTGCC 
AGGGCAGCCT CAAAAGACAC CATATCCAAT AATAGAGAAA AAATTAAAGG CTGGATTAAG 
GAGCAGGCAC ATAAATTTGT TGAGCGTTAT TTTAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCCTTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCT 
TCATTTGAAA TCCAACATAG TGGGTTTGTG AAACAACTGT TGCTGTATTT GACATCTAAG 
AATGAAAAGG ATGCTGTCGG CAGAGAGATC CGGTTAAAAC GTTTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT GGAAGAGTAG AACCAGTGGG CCATGCACCT 
TTGCTGGCAT TGGTTCACAA GATGAACAAC TGTCTCAGCC AAATGGAGCA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGGAACGGA GCTGGAGGAA GAGGATCACA AGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGTCAG TTACAAAGAC ACCCAGACTG TGCAAATGTG 
AAGCAGTGGA AAGGCGGGCC TGTCAAGATT GATCCCCTGG CTCTGGTGCA AGCTATTGAG 
AGATACCTTG TGGTTAGAGG ATATGGAAGA GTAAGAGAAG ATGATGAAGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAGTCT TTGGCTGCTC AATTCTTAAA TTCGGGAAAT 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTAC TACCATATAA CATGACCGTG 
TATCAGGCAG TGCGGCAATT CAGTGTACAG GCTGAAGATG AAAGAGAATC TACTGATGAT 
GAGAGCAATC CTCTGGGAAG AGCTGGAATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGGG AGGATGAAGA AAGTACTAAA GATTGTGTTG GTGGCAAAAG GGGGAGAGCC 
CAAACAGCTC CAACAAAAAC TTCCCCCAGA AATGCAAAGA AGCATGATGA GTTATGGCAT 
GATGGAGTGT GCCCGTCAGT AGCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATCA CCTTTGAAGA CCCATCCTTA GATGTAATAC TTCTTTTAAG AGTTTTACAT 
GCCATCAGTC GATATTGGTA TTACTTGTAT GATAATGCGA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAATTAACA GCAAAAGCAA ATAGGCAGCT TCAAGACCCT 
CTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTCACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGACAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGGGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAC 
AGCAGAGTTG CGCCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTACTG 
AAACAAGCTG AGTCTGTGAT GCAGGACCTT GGCAGCTCTC GGGCCATGTT AGAAATCCAA 
TATGAAAATG AGGTTGGTAC AGGTCTGGGG CCAACACTGG AGTTTTATGC ACTTGTTTCT 
CAGGAACTAC AGAGAGCTGA CTTGTGTCTC TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAC ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCTAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCTCTTGGC 
CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG GAAACTTCAT TGACATCTCA TGATTTATTT 
GACATTGATC CTGTTGTAGC CAGATCAGTA TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTGG AGCAGGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTGGAAACT 
CTTACGATGA ATGGCTGTTC AGTGGAAGAT CTGGGATTGG ATTTTACGCT GCCTGGGTTT 
CCCAACATTG AATTGAAGAA AGGAGGGAAG GATATTCCAG TTACTATCCA CAATTTGGAA 
GAGTACCTGA GATTGGTGAT ATTCTGGGCA CTAAATGAAG GTGTTTGTCG ACAGTTTGAC 
TCATTCAGAG ATGGATTTGA ATCGGTCTTC CCGCTCTGCC ATCTTCAGTA CTTCTACCCA 
GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACATTG 
ATGGAATGCT GCAGGCCTGA TCATGGCTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACT 
GGTAGCCCAC GATTGCCAGT TGGAGGATTT CGGAGTTTAA ATCCACCTCT GACAATTGTG 
CGGAAGACAT TTGAATCAAC AGAAAACCCA GATGACTTCT TACCATCAGT AATGACTTGT 
GTGAACTATC TTAAATTGCC GGACTACTCG AGCATTGACA TAATGCGGGA CAAACTCTTG 
ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249247.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105107.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X40, mRNA.

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CloneID	OMu85016
Clone ID Related Accession (Same CDS sequence)	XM_030249247.1
Accession Version	XM_030249247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAAGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ATCCCTTAGC CAATTCTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAT 
CCGGAACTGG CTAAGTCTTT CATTAAGACC TTGTTTGGTG TTCTCTATGA AGTGTACAGC 
TCCTCAGCAG GCCCTGCGGT CAGACACAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCTGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCAATGC TATCCAGCCA AGACCTGAAG ATAGTAGTTG GAGCTCTTCA GATGGCAGAA 
ATCCTAATGC AAAAGTTACC TGATATTTTT AGTGTTTACT TCAGGAGAGA AGGTGTGATG 
CATCAAGTCA AACATTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCCCC CAAGGCATGT 
ACAAATGGGT CAGGATCCCT GGGATCTACA ACACCAGCCA GCAGTGGGAC TGCAACGGCA 
GCAACCAACG CCTCTGCTGA TCTGGGGTCA CCTAGCTTGC AGCATAGCAG AGATGATTCT 
TTGGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTCA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA ATATTCCCCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCCT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ATATCGAACC AGCACGAACT 
GCAGGAGTTA GTGGTCTTGC CAGGGCAGCC TCAAAAGACA CCATATCCAA TAATAGAGAA 
AAAATTAAAG GCTGGATTAA GGAGCAGGCA CATAAATTTG TTGAGCGTTA TTTTAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCCTTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC TTCATTTGAA ATCCAACATA GTGGGTTTGT GAAACAACTG 
TTGCTGTATT TGACATCTAA GAATGAAAAG GATGCTGTCG GCAGAGAGAT CCGGTTAAAA 
CGTTTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCTGT TGGAAGAGTA 
GAACCAGTGG GCCATGCACC TTTGCTGGCA TTGGTTCACA AGATGAACAA CTGTCTCAGC 
CAAATGGAGC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGGAACGG AGCTGGAGGA 
AGAGGATCAC AAGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGTCA GTTACAAAGA 
CACCCAGACT GTGCAAATGT GAAGCAGTGG AAAGGCGGGC CTGTCAAGAT TGATCCCCTG 
GCTCTGGTGC AAGCTATTGA GAGATACCTT GTGGTTAGAG GATATGGAAG AGTAAGAGAA 
GATGATGAAG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAGTC TTTGGCTGCT 
CAATTCTTAA ATTCGGGAAA TGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTA 
CTACCATATA ACATGACCGT GTATCAGGCA GTGCGGCAAT TCAGTGTACA GGCTGAAGAT 
GAAAGAGAAT CTACTGATGA TGAGAGCAAT CCTCTGGGAA GAGCTGGAAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGG GAGGATGAAG AAAGTACTAA AGATTGTGTT 
GGTGGCAAAA GGGGGAGAGC CCAAACAGCT CCAACAAAAA CTTCCCCCAG AAATGCAAAG 
AAGCATGATG AGTTATGGCA TGATGGAGTG TGCCCGTCAG TAGCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATC ACCTTTGAAG ACCCATCCTT AGATGTAATA 
CTTCTTTTAA GAGTTTTACA TGCCATCAGT CGATATTGGT ATTACTTGTA TGATAATGCG 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAATTAAC AGCAAAAGCA 
AATAGGCAGC TTCAAGACCC TCTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTCACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGACAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGGGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA CAGCAGAGTT GCGCCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTACT GAAACAAGCT GAGTCTGTGA TGCAGGACCT TGGCAGCTCT 
CGGGCCATGT TAGAAATCCA ATATGAAAAT GAGGTTGGTA CAGGTCTGGG GCCAACACTG 
GAGTTTTATG CACTTGTTTC TCAGGAACTA CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA CATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCTA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCTCTTGG CCTGCCCTTT TATAAGTGGA TGCTGCGGCA GGAAACTTCA 
TTGACATCTC ATGATTTATT TGACATTGAT CCTGTTGTAG CCAGATCAGT ATATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTG GAGCAGGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTGGAAAC TCTTACGATG AATGGCTGTT CAGTGGAAGA TCTGGGATTG 
GATTTTACGC TGCCTGGGTT TCCCAACATT GAATTGAAGA AAGGAGGGAA GGATATTCCA 
GTTACTATCC ACAATTTGGA AGAGTACCTG AGATTGGTGA TATTCTGGGC ACTAAATGAA 
GGTGTTTGTC GACAGTTTGA CTCATTCAGA GATGGATTTG AATCGGTCTT CCCGCTCTGC 
CATCTTCAGT ACTTCTACCC AGAAGAGCTG GACCAGCTCC TGTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACATT GATGGAATGC TGCAGGCCTG ATCATGGCTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA CGATTGCCAG TTGGAGGATT TCGGAGTTTA 
AATCCACCTC TGACAATTGT GCGGAAGACA TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTACCATCAG TAATGACTTG TGTGAACTAT CTTAAATTGC CGGACTACTC GAGCATTGAC 
ATAATGCGGG ACAAACTCTT GATAGCAGCA AGAGAAGGCC AACAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105107.1
CDS	363..6425
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249247.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249247.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAAGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ATCCCTTAGC CAATTCTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAT 
CCGGAACTGG CTAAGTCTTT CATTAAGACC TTGTTTGGTG TTCTCTATGA AGTGTACAGC 
TCCTCAGCAG GCCCTGCGGT CAGACACAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCTGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCAATGC TATCCAGCCA AGACCTGAAG ATAGTAGTTG GAGCTCTTCA GATGGCAGAA 
ATCCTAATGC AAAAGTTACC TGATATTTTT AGTGTTTACT TCAGGAGAGA AGGTGTGATG 
CATCAAGTCA AACATTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCCCC CAAGGCATGT 
ACAAATGGGT CAGGATCCCT GGGATCTACA ACACCAGCCA GCAGTGGGAC TGCAACGGCA 
GCAACCAACG CCTCTGCTGA TCTGGGGTCA CCTAGCTTGC AGCATAGCAG AGATGATTCT 
TTGGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTCA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA ATATTCCCCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCCT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ATATCGAACC AGCACGAACT 
GCAGGAGTTA GTGGTCTTGC CAGGGCAGCC TCAAAAGACA CCATATCCAA TAATAGAGAA 
AAAATTAAAG GCTGGATTAA GGAGCAGGCA CATAAATTTG TTGAGCGTTA TTTTAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCCTTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC TTCATTTGAA ATCCAACATA GTGGGTTTGT GAAACAACTG 
TTGCTGTATT TGACATCTAA GAATGAAAAG GATGCTGTCG GCAGAGAGAT CCGGTTAAAA 
CGTTTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCTGT TGGAAGAGTA 
GAACCAGTGG GCCATGCACC TTTGCTGGCA TTGGTTCACA AGATGAACAA CTGTCTCAGC 
CAAATGGAGC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGGAACGG AGCTGGAGGA 
AGAGGATCAC AAGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGTCA GTTACAAAGA 
CACCCAGACT GTGCAAATGT GAAGCAGTGG AAAGGCGGGC CTGTCAAGAT TGATCCCCTG 
GCTCTGGTGC AAGCTATTGA GAGATACCTT GTGGTTAGAG GATATGGAAG AGTAAGAGAA 
GATGATGAAG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAGTC TTTGGCTGCT 
CAATTCTTAA ATTCGGGAAA TGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTA 
CTACCATATA ACATGACCGT GTATCAGGCA GTGCGGCAAT TCAGTGTACA GGCTGAAGAT 
GAAAGAGAAT CTACTGATGA TGAGAGCAAT CCTCTGGGAA GAGCTGGAAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGG GAGGATGAAG AAAGTACTAA AGATTGTGTT 
GGTGGCAAAA GGGGGAGAGC CCAAACAGCT CCAACAAAAA CTTCCCCCAG AAATGCAAAG 
AAGCATGATG AGTTATGGCA TGATGGAGTG TGCCCGTCAG TAGCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATC ACCTTTGAAG ACCCATCCTT AGATGTAATA 
CTTCTTTTAA GAGTTTTACA TGCCATCAGT CGATATTGGT ATTACTTGTA TGATAATGCG 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAATTAAC AGCAAAAGCA 
AATAGGCAGC TTCAAGACCC TCTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTCACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGACAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGGGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA CAGCAGAGTT GCGCCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTACT GAAACAAGCT GAGTCTGTGA TGCAGGACCT TGGCAGCTCT 
CGGGCCATGT TAGAAATCCA ATATGAAAAT GAGGTTGGTA CAGGTCTGGG GCCAACACTG 
GAGTTTTATG CACTTGTTTC TCAGGAACTA CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA CATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCTA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCTCTTGG CCTGCCCTTT TATAAGTGGA TGCTGCGGCA GGAAACTTCA 
TTGACATCTC ATGATTTATT TGACATTGAT CCTGTTGTAG CCAGATCAGT ATATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTG GAGCAGGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTGGAAAC TCTTACGATG AATGGCTGTT CAGTGGAAGA TCTGGGATTG 
GATTTTACGC TGCCTGGGTT TCCCAACATT GAATTGAAGA AAGGAGGGAA GGATATTCCA 
GTTACTATCC ACAATTTGGA AGAGTACCTG AGATTGGTGA TATTCTGGGC ACTAAATGAA 
GGTGTTTGTC GACAGTTTGA CTCATTCAGA GATGGATTTG AATCGGTCTT CCCGCTCTGC 
CATCTTCAGT ACTTCTACCC AGAAGAGCTG GACCAGCTCC TGTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACATT GATGGAATGC TGCAGGCCTG ATCATGGCTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA CGATTGCCAG TTGGAGGATT TCGGAGTTTA 
AATCCACCTC TGACAATTGT GCGGAAGACA TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTACCATCAG TAATGACTTG TGTGAACTAT CTTAAATTGC CGGACTACTC GAGCATTGAC 
ATAATGCGGG ACAAACTCTT GATAGCAGCA AGAGAAGGCC AACAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249259.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105119.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X41, mRNA.

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CloneID	OMu85017
Clone ID Related Accession (Same CDS sequence)	XM_030249259.1
Accession Version	XM_030249259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA GTCCACCAAG 
AAACGCAGTG AGTCCCCACC TGCTGAGCTC CCCAGTTTGA GGAGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA GAGAGGAGCA 
GCTGAGGCCC GGCGGCAAGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GACAGTAAAT 
TCCTCAGCTG CCCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCTCTGTT AGAGGCCAGG 
GGTCTTCCTC CTCACCTATT TGGTCCTCTC GGGCCTCGGA TGTCACAACT TTTCCATAGA 
ACAATTGGAA GCGGAGCTAG TTCTAAGGCC CAGCAGCTTC TTCAAGGACT ACAAGCCAGT 
GATGAAAGTC AACAGCTTCA GGCCGTCATT GAAATGTGTC AGTTATTGGT CATGGGGAAT 
GAGGAGACAC TAGGAGGGTT TCCTGTGAAG AGTGTTGTTC CAGCTTTGAT AACATTACTG 
CAGATGGAAC ATAATTTTGA CATTATGAAT CATGCTTGCC GAGCCTTAAC ATATATGATG 
GAAGCTCTCC CCCGGTCATC TGCTGTTGTA GTGGATGCTA TCCCTGTTTT CTTAGAAAAG 
TTGCAAGTTA TTCAGTGTAT TGATGTGGCA GAGCAGGCCT TGACTGCCTT GGAGATGTTG 
TCACGGCGAC ACAGTAAAGC CATTTTACAA GCGGGTGGTT TGGCAGATTG CTTGTTGTAT 
CTAGAATTCT TCAGCATCAA TGCCCAAAGA AATGCACTAG CAATTGCAGC CAATTGCTGC 
CAGAGTATCA CACCAGATGA GTTTCATTTT GTGGCAGATT CCCTACCATT GCTTACCCAA 
AGGCTAACTC ATCAGGACAA AAAGTCAGTA GAAAGTACTT GCCTTTGTTT TGCACGTTTA 
GTGGACAACT TTCAACATGA GGAGAACTTA CTGCAACAGG TTGCTTCCAA AGATCTACTT 
ACAAATGTTC AACAATTGTT GGTAGTGACT CCACCGATTT TAAGTTCTGG GATGTTTATT 
ATGGTGGTTC GCATGTTTTC TCTGATGTGT TCCAACTGTC CAACATTAGC TGTTCAACTT 
ATGAAACAAA ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAAGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAATCCC 
TTAGCCAATT CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTAATGAAAG AAGATCCGGA ACTGGCTAAG TCTTTCATTA AGACCTTGTT TGGTGTTCTC 
TATGAAGTGT ACAGCTCCTC AGCAGGCCCT GCGGTCAGAC ACAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTTGC TGATGCTGAA CTTCTGAAGG ATGTTCTGAA AAATCATGCT 
GTTTCAAGTC ACATTGCTTC AATGCTATCC AGCCAAGACC TGAAGATAGT AGTTGGAGCT 
CTTCAGATGG CAGAAATCCT AATGCAAAAG TTACCTGATA TTTTTAGTGT TTACTTCAGG 
AGAGAAGGTG TGATGCATCA AGTCAAACAT TTAGCAGAAT CAGAGTCTTT GTTGACAAGT 
CCCCCCAAGG CATGTACAAA TGGGTCAGGA TCCCTGGGAT CTACAACACC AGCCAGCAGT 
GGGACTGCAA CGGCAGCAAC CAACGCCTCT GCTGATCTGG GGTCACCTAG CTTGCAGCAT 
AGCAGAGATG ATTCTTTGGA TCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTCAAGAGA 
AAACGACTGC CAAAACGAGG GCCAAGAAGG CCAAAATATT CCCCTCCAAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACC ACTACTCAGT CACCTAAATC TTCCTTCCTG 
GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG TTAAGCGCAC AGTCCAACAG CAACAATATC 
GAACCAGCAC GAACTGCAGG AGTTAGTGGT CTTGCCAGGG CAGCCTCAAA AGACACCATA 
TCCAATAATA GAGAAAAAAT TAAAGGCTGG ATTAAGGAGC AGGCACATAA ATTTGTTGAG 
CGTTATTTTA GTTCTGAGAA TATGGATGGA AGCAACCCTG CCTTGAATGT CCTTCAGAGA 
CTTTGTGCTG CAACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCAGAT GTTTCTTCAT TTGAAATCCA ACATAGTGGG 
TTTGTGAAAC AACTGTTGCT GTATTTGACA TCTAAGAATG AAAAGGATGC TGTCGGCAGA 
GAGATCCGGT TAAAACGTTT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCTGTTGGAA GAGTAGAACC AGTGGGCCAT GCACCTTTGC TGGCATTGGT TCACAAGATG 
AACAACTGTC TCAGCCAAAT GGAGCAATTT CCAGTCAAAG TACATGATTT CCCTAGTGGG 
AACGGAGCTG GAGGAAGCTT TTCTCTCAAC AGAGGATCAC AAGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGTCA GTTACAAAGA CACCCAGACT GTGCAAATGT GAAGCAGTGG 
AAAGGCGGGC CTGTCAAGAT TGATCCCCTG GCTCTGGTGC AAGCTATTGA GAGATACCTT 
GTGGTTAGAG GTATTTTCCG ATATGGAAGA GTAAGAGAAG ATGATGAAGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAGTCT TTGGCTGCTC AATTCTTAAA TTCGGGAAAT 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTAC TACCATATAA CATGACCGTG 
TATCAGGCAG TGCGGCAATT CAGTGTACAG GCTGAAGATG AAAGAGAATC TACTGATGAT 
GAGAGCAATC CTCTGGGAAG AGCTGGAATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGGG AGGATGAAGA AAGTACTAAA GATTGTGTTG GTGGCAAAAG GGGGAGAGCC 
CAAACAGCTC CAACAAAAAC TTCCCCCAGA AATGCAAAGA AGCATGATGA GTTATGGCAT 
GATGGAGTGT GCCCGTCAGT AGCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATCA CCTTTGAAGA CCCATCCTTA GATGTAATAC TTCTTTTAAG AGTTTTACAT 
GCCATCAGTC GATATTGGTA TTACTTGTAT GATAATGCGA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAATTAACA GCAAAAGCAA ATAGGCAGCT TCAAGACCCT 
CTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTCACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGACAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGGGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAC 
AGCAGAGTTG CGCCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTACTG 
AAACAAGCTG AGTCTGTGAT GCAGGACCTT GGCAGCTCTC GGGCCATGTT AGAAATCCAA 
TATGAAAATG AGGTTGGTAC AGGTCTGGGG CCAACACTGG AGTTTTATGC ACTTGTTTCT 
CAGGAACTAC AGAGAGCTGA CTTGTGTCTC TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAC ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCTAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCTCTTGGC 
CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG GAAACTTCAT TGACATCTCA TGATTTATTT 
GACATTGATC CTGTTGTAGC CAGATCAGTA TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTGG AGCAGGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTGGAAACT 
CTTACGATGA ATGGCTGTTC AGTGGAAGAT CTGGGATTGG ATTTTACGCT GCCTGGGTTT 
CCCAACATTG AATTGAAGAA AGGAGGGAAG GATATTCCAG TTACTATCCA CAATTTGGAA 
GAGTACCTGA GATTGGTGAT ATTCTGGGCA CTAAATGAAG GTGTTTGTCG ACAGTTTGAC 
TCATTCAGAG ATGGATTTGA ATCGGTCTTC CCGCTCTGCC ATCTTCAGTA CTTCTACCCA 
GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACATTG 
ATGGAATGCT GCAGGCCTGA TCATGGCTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACT 
GGTAGCCCAC GATTGCCAGT TGGAGGATTT CGGAGTTTAA ATCCACCTCT GACAATTGTG 
CGGAAGACAT TTGAATCAAC AGAAAACCCA GATGACTTCT TACCATCAGT AATGACTTGT 
GTGAACTATC TTAAATTGCC GGACTACTCG AGCATTGACA TAATGCGGGA CAAACTCTTG 
ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105119.1
CDS	363..6404
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249259.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249259.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA GTCCACCAAG 
AAACGCAGTG AGTCCCCACC TGCTGAGCTC CCCAGTTTGA GGAGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA GAGAGGAGCA 
GCTGAGGCCC GGCGGCAAGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GACAGTAAAT 
TCCTCAGCTG CCCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCTCTGTT AGAGGCCAGG 
GGTCTTCCTC CTCACCTATT TGGTCCTCTC GGGCCTCGGA TGTCACAACT TTTCCATAGA 
ACAATTGGAA GCGGAGCTAG TTCTAAGGCC CAGCAGCTTC TTCAAGGACT ACAAGCCAGT 
GATGAAAGTC AACAGCTTCA GGCCGTCATT GAAATGTGTC AGTTATTGGT CATGGGGAAT 
GAGGAGACAC TAGGAGGGTT TCCTGTGAAG AGTGTTGTTC CAGCTTTGAT AACATTACTG 
CAGATGGAAC ATAATTTTGA CATTATGAAT CATGCTTGCC GAGCCTTAAC ATATATGATG 
GAAGCTCTCC CCCGGTCATC TGCTGTTGTA GTGGATGCTA TCCCTGTTTT CTTAGAAAAG 
TTGCAAGTTA TTCAGTGTAT TGATGTGGCA GAGCAGGCCT TGACTGCCTT GGAGATGTTG 
TCACGGCGAC ACAGTAAAGC CATTTTACAA GCGGGTGGTT TGGCAGATTG CTTGTTGTAT 
CTAGAATTCT TCAGCATCAA TGCCCAAAGA AATGCACTAG CAATTGCAGC CAATTGCTGC 
CAGAGTATCA CACCAGATGA GTTTCATTTT GTGGCAGATT CCCTACCATT GCTTACCCAA 
AGGCTAACTC ATCAGGACAA AAAGTCAGTA GAAAGTACTT GCCTTTGTTT TGCACGTTTA 
GTGGACAACT TTCAACATGA GGAGAACTTA CTGCAACAGG TTGCTTCCAA AGATCTACTT 
ACAAATGTTC AACAATTGTT GGTAGTGACT CCACCGATTT TAAGTTCTGG GATGTTTATT 
ATGGTGGTTC GCATGTTTTC TCTGATGTGT TCCAACTGTC CAACATTAGC TGTTCAACTT 
ATGAAACAAA ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAAGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAATCCC 
TTAGCCAATT CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTAATGAAAG AAGATCCGGA ACTGGCTAAG TCTTTCATTA AGACCTTGTT TGGTGTTCTC 
TATGAAGTGT ACAGCTCCTC AGCAGGCCCT GCGGTCAGAC ACAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTTGC TGATGCTGAA CTTCTGAAGG ATGTTCTGAA AAATCATGCT 
GTTTCAAGTC ACATTGCTTC AATGCTATCC AGCCAAGACC TGAAGATAGT AGTTGGAGCT 
CTTCAGATGG CAGAAATCCT AATGCAAAAG TTACCTGATA TTTTTAGTGT TTACTTCAGG 
AGAGAAGGTG TGATGCATCA AGTCAAACAT TTAGCAGAAT CAGAGTCTTT GTTGACAAGT 
CCCCCCAAGG CATGTACAAA TGGGTCAGGA TCCCTGGGAT CTACAACACC AGCCAGCAGT 
GGGACTGCAA CGGCAGCAAC CAACGCCTCT GCTGATCTGG GGTCACCTAG CTTGCAGCAT 
AGCAGAGATG ATTCTTTGGA TCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTCAAGAGA 
AAACGACTGC CAAAACGAGG GCCAAGAAGG CCAAAATATT CCCCTCCAAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACC ACTACTCAGT CACCTAAATC TTCCTTCCTG 
GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG TTAAGCGCAC AGTCCAACAG CAACAATATC 
GAACCAGCAC GAACTGCAGG AGTTAGTGGT CTTGCCAGGG CAGCCTCAAA AGACACCATA 
TCCAATAATA GAGAAAAAAT TAAAGGCTGG ATTAAGGAGC AGGCACATAA ATTTGTTGAG 
CGTTATTTTA GTTCTGAGAA TATGGATGGA AGCAACCCTG CCTTGAATGT CCTTCAGAGA 
CTTTGTGCTG CAACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCAGAT GTTTCTTCAT TTGAAATCCA ACATAGTGGG 
TTTGTGAAAC AACTGTTGCT GTATTTGACA TCTAAGAATG AAAAGGATGC TGTCGGCAGA 
GAGATCCGGT TAAAACGTTT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCTGTTGGAA GAGTAGAACC AGTGGGCCAT GCACCTTTGC TGGCATTGGT TCACAAGATG 
AACAACTGTC TCAGCCAAAT GGAGCAATTT CCAGTCAAAG TACATGATTT CCCTAGTGGG 
AACGGAGCTG GAGGAAGCTT TTCTCTCAAC AGAGGATCAC AAGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGTCA GTTACAAAGA CACCCAGACT GTGCAAATGT GAAGCAGTGG 
AAAGGCGGGC CTGTCAAGAT TGATCCCCTG GCTCTGGTGC AAGCTATTGA GAGATACCTT 
GTGGTTAGAG GTATTTTCCG ATATGGAAGA GTAAGAGAAG ATGATGAAGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAGTCT TTGGCTGCTC AATTCTTAAA TTCGGGAAAT 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTAC TACCATATAA CATGACCGTG 
TATCAGGCAG TGCGGCAATT CAGTGTACAG GCTGAAGATG AAAGAGAATC TACTGATGAT 
GAGAGCAATC CTCTGGGAAG AGCTGGAATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGGG AGGATGAAGA AAGTACTAAA GATTGTGTTG GTGGCAAAAG GGGGAGAGCC 
CAAACAGCTC CAACAAAAAC TTCCCCCAGA AATGCAAAGA AGCATGATGA GTTATGGCAT 
GATGGAGTGT GCCCGTCAGT AGCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATCA CCTTTGAAGA CCCATCCTTA GATGTAATAC TTCTTTTAAG AGTTTTACAT 
GCCATCAGTC GATATTGGTA TTACTTGTAT GATAATGCGA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAATTAACA GCAAAAGCAA ATAGGCAGCT TCAAGACCCT 
CTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTCACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGACAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGGGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAC 
AGCAGAGTTG CGCCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTACTG 
AAACAAGCTG AGTCTGTGAT GCAGGACCTT GGCAGCTCTC GGGCCATGTT AGAAATCCAA 
TATGAAAATG AGGTTGGTAC AGGTCTGGGG CCAACACTGG AGTTTTATGC ACTTGTTTCT 
CAGGAACTAC AGAGAGCTGA CTTGTGTCTC TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAC ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCTAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCTCTTGGC 
CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG GAAACTTCAT TGACATCTCA TGATTTATTT 
GACATTGATC CTGTTGTAGC CAGATCAGTA TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTGG AGCAGGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTGGAAACT 
CTTACGATGA ATGGCTGTTC AGTGGAAGAT CTGGGATTGG ATTTTACGCT GCCTGGGTTT 
CCCAACATTG AATTGAAGAA AGGAGGGAAG GATATTCCAG TTACTATCCA CAATTTGGAA 
GAGTACCTGA GATTGGTGAT ATTCTGGGCA CTAAATGAAG GTGTTTGTCG ACAGTTTGAC 
TCATTCAGAG ATGGATTTGA ATCGGTCTTC CCGCTCTGCC ATCTTCAGTA CTTCTACCCA 
GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACATTG 
ATGGAATGCT GCAGGCCTGA TCATGGCTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACT 
GGTAGCCCAC GATTGCCAGT TGGAGGATTT CGGAGTTTAA ATCCACCTCT GACAATTGTG 
CGGAAGACAT TTGAATCAAC AGAAAACCCA GATGACTTCT TACCATCAGT AATGACTTGT 
GTGAACTATC TTAAATTGCC GGACTACTCG AGCATTGACA TAATGCGGGA CAAACTCTTG 
ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249267.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105127.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X42, mRNA.

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CloneID	OMu85018
Clone ID Related Accession (Same CDS sequence)	XM_030249267.1
Accession Version	XM_030249267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCTCTGT TAGAGGCCAG GGGTCTTCCT CCTCACCTAT TTGGTCCTCT CGGGCCTCGG 
ATGTCACAAC TTTTCCATAG AACAATTGGA AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT 
CTTCAAGGAC TACAAGCCAG TGATGAAAGT CAACAGCTTC AGGCCGTCAT TGAAATGTGT 
CAGTTATTGG TCATGGGGAA TGAGGAGACA CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT 
CCAGCTTTGA TAACATTACT GCAGATGGAA CATAATTTTG ACATTATGAA TCATGCTTGC 
CGAGCCTTAA CATATATGAT GGAAGCTCTC CCCCGGTCAT CTGCTGTTGT AGTGGATGCT 
ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT ATTCAGTGTA TTGATGTGGC AGAGCAGGCC 
TTGACTGCCT TGGAGATGTT GTCACGGCGA CACAGTAAAG CCATTTTACA AGCGGGTGGT 
TTGGCAGATT GCTTGTTGTA TCTAGAATTC TTCAGCATCA ATGCCCAAAG AAATGCACTA 
GCAATTGCAG CCAATTGCTG CCAGAGTATC ACACCAGATG AGTTTCATTT TGTGGCAGAT 
TCCCTACCAT TGCTTACCCA AAGGCTAACT CATCAGGACA AAAAGTCAGT AGAAAGTACT 
TGCCTTTGTT TTGCACGTTT AGTGGACAAC TTTCAACATG AGGAGAACTT ACTGCAACAG 
GTTGCTTCCA AAGATCTACT TACAAATGTT CAACAATTGT TGGTAGTGAC TCCACCGATT 
TTAAGTTCTG GGATGTTTAT TATGGTGGTT CGCATGTTTT CTCTGATGTG TTCCAACTGT 
CCAACATTAG CTGTTCAACT TATGAAACAA AGCTTTACAG ACATTGCAGA AACACTTCAC 
TTCCTGCTAT GTGGTGCCTC CAATGGAAGT TGTCAAGAGC AGATTGACCT TGTTCCACGA 
AGTCCTCAAG AGCTGTATGA GCTGACATCT TTGATTTGTG AACTTATGCC ATGTTTACCA 
AAAGAAGGCA TCTTCGCAGT TGATACCATG TTGAAAAAGG GAAATGCACA GAACACAGAT 
GGTGCAATAT GGCAGTGGCG GGATGACCGA GGCCTCTGGC ATCCCTATAA CAGGATTGAC 
AGCCGGATCA TTGAGGTAGC AGCCCATCAG GTCGGTGAGG ATGAGATAAG CTTGTCCACT 
CTGGGACGAG TTTATACTAT TGATTTTAAT TCTATGCAGC AAATCAATGA AGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAT CCCTTAGCCA ATTCTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTAATGA AAGAAGATCC GGAACTGGCT 
AAGTCTTTCA TTAAGACCTT GTTTGGTGTT CTCTATGAAG TGTACAGCTC CTCAGCAGGC 
CCTGCGGTCA GACACAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCTGATGCT 
GAACTTCTGA AGGATGTTCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCAATGCTA 
TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA GCTCTTCAGA TGGCAGAAAT CCTAATGCAA 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGGAGAGAAG GTGTGATGCA TCAAGTCAAA 
CATTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCCCCCA AGGCATGTAC AAATGGGTCA 
GGATCCCTGG GATCTACAAC ACCAGCCAGC AGTGGGACTG CAACGGCAGC AACCAACGCC 
TCTGCTGATC TGGGGTCACC TAGCTTGCAG CATAGCAGAG ATGATTCTTT GGATCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTCAAG AGAAAACGAC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAAT ATTCCCCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACCACTACTC AGTCACCTAA ATCTTCCTTC CTGGCAAGCT TGAATCCAAA AACATGGGGA 
AGGTTAAGCG CACAGTCCAA CAGCAACAAT ATCGAACCAG CACGAACTGC AGGAGTTAGT 
GGTCTTGCCA GGGCAGCCTC AAAAGACACC ATATCCAATA ATAGAGAAAA AATTAAAGGC 
TGGATTAAGG AGCAGGCACA TAAATTTGTT GAGCGTTATT TTAGTTCTGA GAATATGGAT 
GGAAGCAACC CTGCCTTGAA TGTCCTTCAG AGACTTTGTG CTGCAACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCA 
GATGTTTCTT CATTTGAAAT CCAACATAGT GGGTTTGTGA AACAACTGTT GCTGTATTTG 
ACATCTAAGA ATGAAAAGGA TGCTGTCGGC AGAGAGATCC GGTTAAAACG TTTCCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC 
CATGCACCTT TGCTGGCATT GGTTCACAAG ATGAACAACT GTCTCAGCCA AATGGAGCAA 
TTTCCAGTCA AAGTACATGA TTTCCCTAGT GGGAACGGAG CTGGAGGAAG CTTTTCTCTC 
AACAGAGGAT CACAAGCTTT AAAATTTTTC AACACACATC AGTTAAAATG TCAGTTACAA 
AGACACCCAG ACTGTGCAAA TGTGAAGCAG TGGAAAGGCG GGCCTGTCAA GATTGATCCC 
CTGGCTCTGG TGCAAGCTAT TGAGAGATAC CTTGTGGTTA GAGGTATTTT CCGATATGGA 
AGAGTAAGAG AAGATGATGA AGATAGTGAT GATGATGGGT CAGATGAAGA AATAGATGAG 
TCTTTGGCTG CTCAATTCTT AAATTCGGGA AATGTAAGGC ACAGGCTACA GTTTTATATT 
GGAGAACATT TACTACCATA TAACATGACC GTGTATCAGG CAGTGCGGCA ATTCAGTGTA 
CAGGCTGAAG ATGAAAGAGA ATCTACTGAT GATGAGAGCA ATCCTCTGGG AAGAGCTGGA 
ATTTGGACAA AGACTCATAC AATATGGTAC AAACCTGTGA GGGAGGATGA AGAAAGTACT 
AAAGATTGTG TTGGTGGCAA AAGGGGGAGA GCCCAAACAG CTCCAACAAA AACTTCCCCC 
AGAAATGCAA AGAAGCATGA TGAGTTATGG CATGATGGAG TGTGCCCGTC AGTAGCAAAT 
CCTTTAGAAG TTTACCTCAT TCCCACACCA CCTGAAAATA TCACCTTTGA AGACCCATCC 
TTAGATGTAA TACTTCTTTT AAGAGTTTTA CATGCCATCA GTCGATATTG GTATTACTTG 
TATGATAATG CGATGTGCAA GGAGATTATT CCAACTAGTG AATTTATTAA CAGTAAATTA 
ACAGCAAAAG CAAATAGGCA GCTTCAAGAC CCTCTAGTAA TCATGACAGG AAACATCCCA 
ACATGGCTCA CTGAGCTAGG AAAAACCTGC CCATTTTTCT TTCCTTTTGA TACCCGACAA 
ATGCTTTTTT ATGTAACTGC ATTTGATCGG GACCGGGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAAA TCAACCAGTC TGATTCTCAA GACAGCAGAG TTGCGCCTAG ATTGGATAGA 
AAAAAACGTA CTGTGAACAG AGAGGAGCTA CTGAAACAAG CTGAGTCTGT GATGCAGGAC 
CTTGGCAGCT CTCGGGCCAT GTTAGAAATC CAATATGAAA ATGAGGTTGG TACAGGTCTG 
GGGCCAACAC TGGAGTTTTA TGCACTTGTT TCTCAGGAAC TACAGAGAGC TGACTTGTGT 
CTCTGGAGGG GTGAAGAAGT AACTCTTAGC AATCCAAAAG GAAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAGGG CCTGTTTGCG CTTCCCTTTG GTAGGACAGC TAAGCCAGCT 
CACATCGCAA AGGTTAAGAT GAAGTTTCGC TTCTTAGGAA AATTAATGGC TAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCTCTT GGCCTGCCCT TTTATAAGTG GATGCTGCGG 
CAGGAAACTT CATTGACATC TCATGATTTA TTTGACATTG ATCCTGTTGT AGCCAGATCA 
GTATATCACC TAGAAGACAT TGTCAGACAG AAGAAAAGAC TGGAGCAGGA TAAATCCCAG 
ACCAAAGAGA GTCTACAATA TGCATTGGAA ACTCTTACGA TGAATGGCTG TTCAGTGGAA 
GATCTGGGAT TGGATTTTAC GCTGCCTGGG TTTCCCAACA TTGAATTGAA GAAAGGAGGG 
AAGGATATTC CAGTTACTAT CCACAATTTG GAAGAGTACC TGAGATTGGT GATATTCTGG 
GCACTAAATG AAGGTGTTTG TCGACAGTTT GACTCATTCA GAGATGGATT TGAATCGGTC 
TTCCCGCTCT GCCATCTTCA GTACTTCTAC CCAGAAGAGC TGGACCAGCT CCTGTGTGGC 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA TTGATGGAAT GCTGCAGGCC TGATCATGGC 
TATACTCATG ACAGTCGGGC TGTGAAGTTC TTGTTTGAGA TTCTCAGTAG TTTTGATAAT 
GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG ACTGGTAGCC CACGATTGCC AGTTGGAGGA 
TTTCGGAGTT TAAATCCACC TCTGACAATT GTGCGGAAGA CATTTGAATC AACAGAAAAC 
CCAGATGACT TCTTACCATC AGTAATGACT TGTGTGAACT ATCTTAAATT GCCGGACTAC 
TCGAGCATTG ACATAATGCG GGACAAACTC TTGATAGCAG CAAGAGAAGG CCAACAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105127.1
CDS	363..6377
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249267.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249267.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCTCTGT TAGAGGCCAG GGGTCTTCCT CCTCACCTAT TTGGTCCTCT CGGGCCTCGG 
ATGTCACAAC TTTTCCATAG AACAATTGGA AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT 
CTTCAAGGAC TACAAGCCAG TGATGAAAGT CAACAGCTTC AGGCCGTCAT TGAAATGTGT 
CAGTTATTGG TCATGGGGAA TGAGGAGACA CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT 
CCAGCTTTGA TAACATTACT GCAGATGGAA CATAATTTTG ACATTATGAA TCATGCTTGC 
CGAGCCTTAA CATATATGAT GGAAGCTCTC CCCCGGTCAT CTGCTGTTGT AGTGGATGCT 
ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT ATTCAGTGTA TTGATGTGGC AGAGCAGGCC 
TTGACTGCCT TGGAGATGTT GTCACGGCGA CACAGTAAAG CCATTTTACA AGCGGGTGGT 
TTGGCAGATT GCTTGTTGTA TCTAGAATTC TTCAGCATCA ATGCCCAAAG AAATGCACTA 
GCAATTGCAG CCAATTGCTG CCAGAGTATC ACACCAGATG AGTTTCATTT TGTGGCAGAT 
TCCCTACCAT TGCTTACCCA AAGGCTAACT CATCAGGACA AAAAGTCAGT AGAAAGTACT 
TGCCTTTGTT TTGCACGTTT AGTGGACAAC TTTCAACATG AGGAGAACTT ACTGCAACAG 
GTTGCTTCCA AAGATCTACT TACAAATGTT CAACAATTGT TGGTAGTGAC TCCACCGATT 
TTAAGTTCTG GGATGTTTAT TATGGTGGTT CGCATGTTTT CTCTGATGTG TTCCAACTGT 
CCAACATTAG CTGTTCAACT TATGAAACAA AGCTTTACAG ACATTGCAGA AACACTTCAC 
TTCCTGCTAT GTGGTGCCTC CAATGGAAGT TGTCAAGAGC AGATTGACCT TGTTCCACGA 
AGTCCTCAAG AGCTGTATGA GCTGACATCT TTGATTTGTG AACTTATGCC ATGTTTACCA 
AAAGAAGGCA TCTTCGCAGT TGATACCATG TTGAAAAAGG GAAATGCACA GAACACAGAT 
GGTGCAATAT GGCAGTGGCG GGATGACCGA GGCCTCTGGC ATCCCTATAA CAGGATTGAC 
AGCCGGATCA TTGAGGTAGC AGCCCATCAG GTCGGTGAGG ATGAGATAAG CTTGTCCACT 
CTGGGACGAG TTTATACTAT TGATTTTAAT TCTATGCAGC AAATCAATGA AGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAT CCCTTAGCCA ATTCTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTAATGA AAGAAGATCC GGAACTGGCT 
AAGTCTTTCA TTAAGACCTT GTTTGGTGTT CTCTATGAAG TGTACAGCTC CTCAGCAGGC 
CCTGCGGTCA GACACAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCTGATGCT 
GAACTTCTGA AGGATGTTCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCAATGCTA 
TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA GCTCTTCAGA TGGCAGAAAT CCTAATGCAA 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGGAGAGAAG GTGTGATGCA TCAAGTCAAA 
CATTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCCCCCA AGGCATGTAC AAATGGGTCA 
GGATCCCTGG GATCTACAAC ACCAGCCAGC AGTGGGACTG CAACGGCAGC AACCAACGCC 
TCTGCTGATC TGGGGTCACC TAGCTTGCAG CATAGCAGAG ATGATTCTTT GGATCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTCAAG AGAAAACGAC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAAT ATTCCCCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACCACTACTC AGTCACCTAA ATCTTCCTTC CTGGCAAGCT TGAATCCAAA AACATGGGGA 
AGGTTAAGCG CACAGTCCAA CAGCAACAAT ATCGAACCAG CACGAACTGC AGGAGTTAGT 
GGTCTTGCCA GGGCAGCCTC AAAAGACACC ATATCCAATA ATAGAGAAAA AATTAAAGGC 
TGGATTAAGG AGCAGGCACA TAAATTTGTT GAGCGTTATT TTAGTTCTGA GAATATGGAT 
GGAAGCAACC CTGCCTTGAA TGTCCTTCAG AGACTTTGTG CTGCAACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCA 
GATGTTTCTT CATTTGAAAT CCAACATAGT GGGTTTGTGA AACAACTGTT GCTGTATTTG 
ACATCTAAGA ATGAAAAGGA TGCTGTCGGC AGAGAGATCC GGTTAAAACG TTTCCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC 
CATGCACCTT TGCTGGCATT GGTTCACAAG ATGAACAACT GTCTCAGCCA AATGGAGCAA 
TTTCCAGTCA AAGTACATGA TTTCCCTAGT GGGAACGGAG CTGGAGGAAG CTTTTCTCTC 
AACAGAGGAT CACAAGCTTT AAAATTTTTC AACACACATC AGTTAAAATG TCAGTTACAA 
AGACACCCAG ACTGTGCAAA TGTGAAGCAG TGGAAAGGCG GGCCTGTCAA GATTGATCCC 
CTGGCTCTGG TGCAAGCTAT TGAGAGATAC CTTGTGGTTA GAGGTATTTT CCGATATGGA 
AGAGTAAGAG AAGATGATGA AGATAGTGAT GATGATGGGT CAGATGAAGA AATAGATGAG 
TCTTTGGCTG CTCAATTCTT AAATTCGGGA AATGTAAGGC ACAGGCTACA GTTTTATATT 
GGAGAACATT TACTACCATA TAACATGACC GTGTATCAGG CAGTGCGGCA ATTCAGTGTA 
CAGGCTGAAG ATGAAAGAGA ATCTACTGAT GATGAGAGCA ATCCTCTGGG AAGAGCTGGA 
ATTTGGACAA AGACTCATAC AATATGGTAC AAACCTGTGA GGGAGGATGA AGAAAGTACT 
AAAGATTGTG TTGGTGGCAA AAGGGGGAGA GCCCAAACAG CTCCAACAAA AACTTCCCCC 
AGAAATGCAA AGAAGCATGA TGAGTTATGG CATGATGGAG TGTGCCCGTC AGTAGCAAAT 
CCTTTAGAAG TTTACCTCAT TCCCACACCA CCTGAAAATA TCACCTTTGA AGACCCATCC 
TTAGATGTAA TACTTCTTTT AAGAGTTTTA CATGCCATCA GTCGATATTG GTATTACTTG 
TATGATAATG CGATGTGCAA GGAGATTATT CCAACTAGTG AATTTATTAA CAGTAAATTA 
ACAGCAAAAG CAAATAGGCA GCTTCAAGAC CCTCTAGTAA TCATGACAGG AAACATCCCA 
ACATGGCTCA CTGAGCTAGG AAAAACCTGC CCATTTTTCT TTCCTTTTGA TACCCGACAA 
ATGCTTTTTT ATGTAACTGC ATTTGATCGG GACCGGGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAAA TCAACCAGTC TGATTCTCAA GACAGCAGAG TTGCGCCTAG ATTGGATAGA 
AAAAAACGTA CTGTGAACAG AGAGGAGCTA CTGAAACAAG CTGAGTCTGT GATGCAGGAC 
CTTGGCAGCT CTCGGGCCAT GTTAGAAATC CAATATGAAA ATGAGGTTGG TACAGGTCTG 
GGGCCAACAC TGGAGTTTTA TGCACTTGTT TCTCAGGAAC TACAGAGAGC TGACTTGTGT 
CTCTGGAGGG GTGAAGAAGT AACTCTTAGC AATCCAAAAG GAAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAGGG CCTGTTTGCG CTTCCCTTTG GTAGGACAGC TAAGCCAGCT 
CACATCGCAA AGGTTAAGAT GAAGTTTCGC TTCTTAGGAA AATTAATGGC TAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCTCTT GGCCTGCCCT TTTATAAGTG GATGCTGCGG 
CAGGAAACTT CATTGACATC TCATGATTTA TTTGACATTG ATCCTGTTGT AGCCAGATCA 
GTATATCACC TAGAAGACAT TGTCAGACAG AAGAAAAGAC TGGAGCAGGA TAAATCCCAG 
ACCAAAGAGA GTCTACAATA TGCATTGGAA ACTCTTACGA TGAATGGCTG TTCAGTGGAA 
GATCTGGGAT TGGATTTTAC GCTGCCTGGG TTTCCCAACA TTGAATTGAA GAAAGGAGGG 
AAGGATATTC CAGTTACTAT CCACAATTTG GAAGAGTACC TGAGATTGGT GATATTCTGG 
GCACTAAATG AAGGTGTTTG TCGACAGTTT GACTCATTCA GAGATGGATT TGAATCGGTC 
TTCCCGCTCT GCCATCTTCA GTACTTCTAC CCAGAAGAGC TGGACCAGCT CCTGTGTGGC 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA TTGATGGAAT GCTGCAGGCC TGATCATGGC 
TATACTCATG ACAGTCGGGC TGTGAAGTTC TTGTTTGAGA TTCTCAGTAG TTTTGATAAT 
GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG ACTGGTAGCC CACGATTGCC AGTTGGAGGA 
TTTCGGAGTT TAAATCCACC TCTGACAATT GTGCGGAAGA CATTTGAATC AACAGAAAAC 
CCAGATGACT TCTTACCATC AGTAATGACT TGTGTGAACT ATCTTAAATT GCCGGACTAC 
TCGAGCATTG ACATAATGCG GGACAAACTC TTGATAGCAG CAAGAGAAGG CCAACAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249285.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105145.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X43, mRNA.

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CloneID	OMu85019
Clone ID Related Accession (Same CDS sequence)	XM_030249285.1
Accession Version	XM_030249285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA GACATCAAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAGTCCAC CAAGAAACGC 
AGTGAGTCCC CACCTGCTGA GCTCCCCAGT TTGAGGAGGA GCACACGCCA AAAGACCACG 
GGCTCCTGTG CTAGTACCAG TCGGCGAGGC TCTGGCCTGG GCAAGAGAGG AGCAGCTGAG 
GCCCGGCGGC AAGAGAAAAT GGCAGACCCT GAAAGCAACC AGGAGACAGT AAATTCCTCA 
GCTGCCCGGA CAGATGAAGC TCCCCAAGGA GCTGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTTGGCATGA CCACCTCTGG GGAGAGTGAA TCGGATGATT CTGAGATGGG ACGATTACAA 
GCTCTGTTAG AGGCCAGGGG TCTTCCTCCT CACCTATTTG GTCCTCTCGG GCCTCGGATG 
TCACAACTTT TCCATAGAAC AATTGGAAGC GGAGCTAGTT CTAAGGCCCA GCAGCTTCTT 
CAAGGACTAC AAGCCAGTGA TGAAAGTCAA CAGCTTCAGG CCGTCATTGA AATGTGTCAG 
TTATTGGTCA TGGGGAATGA GGAGACACTA GGAGGGTTTC CTGTGAAGAG TGTTGTTCCA 
GCTTTGATAA CATTACTGCA GATGGAACAT AATTTTGACA TTATGAATCA TGCTTGCCGA 
GCCTTAACAT ATATGATGGA AGCTCTCCCC CGGTCATCTG CTGTTGTAGT GGATGCTATC 
CCTGTTTTCT TAGAAAAGTT GCAAGTTATT CAGTGTATTG ATGTGGCAGA GCAGGCCTTG 
ACTGCCTTGG AGATGTTGTC ACGGCGACAC AGTAAAGCCA TTTTACAAGC GGGTGGTTTG 
GCAGATTGCT TGTTGTATCT AGAATTCTTC AGCATCAATG CCCAAAGAAA TGCACTAGCA 
ATTGCAGCCA ATTGCTGCCA GAGTATCACA CCAGATGAGT TTCATTTTGT GGCAGATTCC 
CTACCATTGC TTACCCAAAG GCTAACTCAT CAGGACAAAA AGTCAGTAGA AAGTACTTGC 
CTTTGTTTTG CACGTTTAGT GGACAACTTT CAACATGAGG AGAACTTACT GCAACAGGTT 
GCTTCCAAAG ATCTACTTAC AAATGTTCAA CAATTGTTGG TAGTGACTCC ACCGATTTTA 
AGTTCTGGGA TGTTTATTAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAACTGTCCA 
ACATTAGCTG TTCAACTTAT GAAACAAAGC TTTACAGACA TTGCAGAAAC ACTTCACTTC 
CTGCTATGTG GTGCCTCCAA TGGAAGTTGT CAAGAGCAGA TTGACCTTGT TCCACGAAGT 
CCTCAAGAGC TGTATGAGCT GACATCTTTG ATTTGTGAAC TTATGCCATG TTTACCAAAA 
GAAGGCATCT TCGCAGTTGA TACCATGTTG AAAAAGGGAA ATGCACAGAA CACAGATGGT 
GCAATATGGC AGTGGCGGGA TGACCGAGGC CTCTGGCATC CCTATAACAG GATTGACAGC 
CGGATCATTG AGGTAGCAGC CCATCAGGTC GGTGAGGATG AGATAAGCTT GTCCACTCTG 
GGACGAGTTT ATACTATTGA TTTTAATTCT ATGCAGCAAA TCAATGAAGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAATCCC TTAGCCAATT CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTAATGAAAG AAGATCCGGA ACTGGCTAAG 
TCTTTCATTA AGACCTTGTT TGGTGTTCTC TATGAAGTGT ACAGCTCCTC AGCAGGCCCT 
GCGGTCAGAC ACAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC TGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC AATGCTATCC 
AGCCAAGACC TGAAGATAGT AGTTGGAGCT CTTCAGATGG CAGAAATCCT AATGCAAAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGG AGAGAAGGTG TGATGCATCA AGTCAAACAT 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCCCCCAAGG CATGTACAAA TGGGTCAGGA 
TCCCTGGGAT CTACAACACC AGCCAGCAGT GGGACTGCAA CGGCAGCAAC CAACGCCTCT 
GCTGATCTGG GGTCACCTAG CTTGCAGCAT AGCAGAGATG ATTCTTTGGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTCAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAATATT CCCCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCCTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAATATC GAACCAGCAC GAACTGCAGG AGTTAGTGGT 
CTTGCCAGGG CAGCCTCAAA AGACACCATA TCCAATAATA GAGAAAAAAT TAAAGGCTGG 
ATTAAGGAGC AGGCACATAA ATTTGTTGAG CGTTATTTTA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CCTTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCTTCAT TTGAAATCCA ACATAGTGGG TTTGTGAAAC AACTGTTGCT GTATTTGACA 
TCTAAGAATG AAAAGGATGC TGTCGGCAGA GAGATCCGGT TAAAACGTTT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCTGTTGGAA GAGTAGAACC AGTGGGCCAT 
GCACCTTTGC TGGCATTGGT TCACAAGATG AACAACTGTC TCAGCCAAAT GGAGCAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGG AACGGAGCTG GAGGAAGCTT TTCTCTCAAC 
AGAGGATCAC AAGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGTCA GTTACAAAGA 
CACCCAGACT GTGCAAATGT GAAGCAGTGG AAAGGCGGGC CTGTCAAGAT TGATCCCCTG 
GCTCTGGTGC AAGCTATTGA GAGATACCTT GTGGTTAGAG GTATTTTCCG ATATGGAAGA 
GTAAGAGAAG ATGATGAAGA TAGTGATGAT GATGGGTCAG ATGAAGAAAT AGATGAGTCT 
TTGGCTGCTC AATTCTTAAA TTCGGGAAAT GTAAGGCACA GGCTACAGTT TTATATTGGA 
GAACATTTAC TACCATATAA CATGACCGTG TATCAGGCAG TGCGGCAATT CAGTGTACAG 
GCTGAAGATG AAAGAGAATC TACTGATGAT GAGAGCAATC CTCTGGGAAG AGCTGGAATT 
TGGACAAAGA CTCATACAAT ATGGTACAAA CCTGTGAGGG AGGATGAAGA AAGTACTAAA 
GATTGTGTTG GTGGCAAAAG GGGGAGAGCC CAAACAGCTC CAACAAAAAC TTCCCCCAGA 
AATGCAAAGA AGCATGATGA GTTATGGCAT GATGGAGTGT GCCCGTCAGT AGCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATCA CCTTTGAAGA CCCATCCTTA 
GATGTAATAC TTCTTTTAAG AGTTTTACAT GCCATCAGTC GATATTGGTA TTACTTGTAT 
GATAATGCGA TGTGCAAGGA GATTATTCCA ACTAGTGAAT TTATTAACAG TAAATTAACA 
GCAAAAGCAA ATAGGCAGCT TCAAGACCCT CTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTCACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGACAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGGGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAC AGCAGAGTTG CGCCTAGATT GGATAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTACTG AAACAAGCTG AGTCTGTGAT GCAGGACCTT 
GGCAGCTCTC GGGCCATGTT AGAAATCCAA TATGAAAATG AGGTTGGTAC AGGTCTGGGG 
CCAACACTGG AGTTTTATGC ACTTGTTTCT CAGGAACTAC AGAGAGCTGA CTTGTGTCTC 
TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAC 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCTAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCTCTTGGC CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG 
GAAACTTCAT TGACATCTCA TGATTTATTT GACATTGATC CTGTTGTAGC CAGATCAGTA 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTGG AGCAGGATAA ATCCCAGACC 
AAAGAGAGTC TACAATATGC ATTGGAAACT CTTACGATGA ATGGCTGTTC AGTGGAAGAT 
CTGGGATTGG ATTTTACGCT GCCTGGGTTT CCCAACATTG AATTGAAGAA AGGAGGGAAG 
GATATTCCAG TTACTATCCA CAATTTGGAA GAGTACCTGA GATTGGTGAT ATTCTGGGCA 
CTAAATGAAG GTGTTTGTCG ACAGTTTGAC TCATTCAGAG ATGGATTTGA ATCGGTCTTC 
CCGCTCTGCC ATCTTCAGTA CTTCTACCCA GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACATTG ATGGAATGCT GCAGGCCTGA TCATGGCTAT 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TGTTTCTCCA GTTTGTGACT GGTAGCCCAC GATTGCCAGT TGGAGGATTT 
CGGAGTTTAA ATCCACCTCT GACAATTGTG CGGAAGACAT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TACCATCAGT AATGACTTGT GTGAACTATC TTAAATTGCC GGACTACTCG 
AGCATTGACA TAATGCGGGA CAAACTCTTG ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105145.1
CDS	369..6380
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249285.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X43, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249285.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA GACATCAAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAGTCCAC CAAGAAACGC 
AGTGAGTCCC CACCTGCTGA GCTCCCCAGT TTGAGGAGGA GCACACGCCA AAAGACCACG 
GGCTCCTGTG CTAGTACCAG TCGGCGAGGC TCTGGCCTGG GCAAGAGAGG AGCAGCTGAG 
GCCCGGCGGC AAGAGAAAAT GGCAGACCCT GAAAGCAACC AGGAGACAGT AAATTCCTCA 
GCTGCCCGGA CAGATGAAGC TCCCCAAGGA GCTGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTTGGCATGA CCACCTCTGG GGAGAGTGAA TCGGATGATT CTGAGATGGG ACGATTACAA 
GCTCTGTTAG AGGCCAGGGG TCTTCCTCCT CACCTATTTG GTCCTCTCGG GCCTCGGATG 
TCACAACTTT TCCATAGAAC AATTGGAAGC GGAGCTAGTT CTAAGGCCCA GCAGCTTCTT 
CAAGGACTAC AAGCCAGTGA TGAAAGTCAA CAGCTTCAGG CCGTCATTGA AATGTGTCAG 
TTATTGGTCA TGGGGAATGA GGAGACACTA GGAGGGTTTC CTGTGAAGAG TGTTGTTCCA 
GCTTTGATAA CATTACTGCA GATGGAACAT AATTTTGACA TTATGAATCA TGCTTGCCGA 
GCCTTAACAT ATATGATGGA AGCTCTCCCC CGGTCATCTG CTGTTGTAGT GGATGCTATC 
CCTGTTTTCT TAGAAAAGTT GCAAGTTATT CAGTGTATTG ATGTGGCAGA GCAGGCCTTG 
ACTGCCTTGG AGATGTTGTC ACGGCGACAC AGTAAAGCCA TTTTACAAGC GGGTGGTTTG 
GCAGATTGCT TGTTGTATCT AGAATTCTTC AGCATCAATG CCCAAAGAAA TGCACTAGCA 
ATTGCAGCCA ATTGCTGCCA GAGTATCACA CCAGATGAGT TTCATTTTGT GGCAGATTCC 
CTACCATTGC TTACCCAAAG GCTAACTCAT CAGGACAAAA AGTCAGTAGA AAGTACTTGC 
CTTTGTTTTG CACGTTTAGT GGACAACTTT CAACATGAGG AGAACTTACT GCAACAGGTT 
GCTTCCAAAG ATCTACTTAC AAATGTTCAA CAATTGTTGG TAGTGACTCC ACCGATTTTA 
AGTTCTGGGA TGTTTATTAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAACTGTCCA 
ACATTAGCTG TTCAACTTAT GAAACAAAGC TTTACAGACA TTGCAGAAAC ACTTCACTTC 
CTGCTATGTG GTGCCTCCAA TGGAAGTTGT CAAGAGCAGA TTGACCTTGT TCCACGAAGT 
CCTCAAGAGC TGTATGAGCT GACATCTTTG ATTTGTGAAC TTATGCCATG TTTACCAAAA 
GAAGGCATCT TCGCAGTTGA TACCATGTTG AAAAAGGGAA ATGCACAGAA CACAGATGGT 
GCAATATGGC AGTGGCGGGA TGACCGAGGC CTCTGGCATC CCTATAACAG GATTGACAGC 
CGGATCATTG AGGTAGCAGC CCATCAGGTC GGTGAGGATG AGATAAGCTT GTCCACTCTG 
GGACGAGTTT ATACTATTGA TTTTAATTCT ATGCAGCAAA TCAATGAAGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAATCCC TTAGCCAATT CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTAATGAAAG AAGATCCGGA ACTGGCTAAG 
TCTTTCATTA AGACCTTGTT TGGTGTTCTC TATGAAGTGT ACAGCTCCTC AGCAGGCCCT 
GCGGTCAGAC ACAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC TGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC AATGCTATCC 
AGCCAAGACC TGAAGATAGT AGTTGGAGCT CTTCAGATGG CAGAAATCCT AATGCAAAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGG AGAGAAGGTG TGATGCATCA AGTCAAACAT 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCCCCCAAGG CATGTACAAA TGGGTCAGGA 
TCCCTGGGAT CTACAACACC AGCCAGCAGT GGGACTGCAA CGGCAGCAAC CAACGCCTCT 
GCTGATCTGG GGTCACCTAG CTTGCAGCAT AGCAGAGATG ATTCTTTGGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTCAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAATATT CCCCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCCTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAATATC GAACCAGCAC GAACTGCAGG AGTTAGTGGT 
CTTGCCAGGG CAGCCTCAAA AGACACCATA TCCAATAATA GAGAAAAAAT TAAAGGCTGG 
ATTAAGGAGC AGGCACATAA ATTTGTTGAG CGTTATTTTA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CCTTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCTTCAT TTGAAATCCA ACATAGTGGG TTTGTGAAAC AACTGTTGCT GTATTTGACA 
TCTAAGAATG AAAAGGATGC TGTCGGCAGA GAGATCCGGT TAAAACGTTT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCTGTTGGAA GAGTAGAACC AGTGGGCCAT 
GCACCTTTGC TGGCATTGGT TCACAAGATG AACAACTGTC TCAGCCAAAT GGAGCAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGG AACGGAGCTG GAGGAAGCTT TTCTCTCAAC 
AGAGGATCAC AAGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGTCA GTTACAAAGA 
CACCCAGACT GTGCAAATGT GAAGCAGTGG AAAGGCGGGC CTGTCAAGAT TGATCCCCTG 
GCTCTGGTGC AAGCTATTGA GAGATACCTT GTGGTTAGAG GTATTTTCCG ATATGGAAGA 
GTAAGAGAAG ATGATGAAGA TAGTGATGAT GATGGGTCAG ATGAAGAAAT AGATGAGTCT 
TTGGCTGCTC AATTCTTAAA TTCGGGAAAT GTAAGGCACA GGCTACAGTT TTATATTGGA 
GAACATTTAC TACCATATAA CATGACCGTG TATCAGGCAG TGCGGCAATT CAGTGTACAG 
GCTGAAGATG AAAGAGAATC TACTGATGAT GAGAGCAATC CTCTGGGAAG AGCTGGAATT 
TGGACAAAGA CTCATACAAT ATGGTACAAA CCTGTGAGGG AGGATGAAGA AAGTACTAAA 
GATTGTGTTG GTGGCAAAAG GGGGAGAGCC CAAACAGCTC CAACAAAAAC TTCCCCCAGA 
AATGCAAAGA AGCATGATGA GTTATGGCAT GATGGAGTGT GCCCGTCAGT AGCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATCA CCTTTGAAGA CCCATCCTTA 
GATGTAATAC TTCTTTTAAG AGTTTTACAT GCCATCAGTC GATATTGGTA TTACTTGTAT 
GATAATGCGA TGTGCAAGGA GATTATTCCA ACTAGTGAAT TTATTAACAG TAAATTAACA 
GCAAAAGCAA ATAGGCAGCT TCAAGACCCT CTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTCACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGACAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGGGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAC AGCAGAGTTG CGCCTAGATT GGATAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTACTG AAACAAGCTG AGTCTGTGAT GCAGGACCTT 
GGCAGCTCTC GGGCCATGTT AGAAATCCAA TATGAAAATG AGGTTGGTAC AGGTCTGGGG 
CCAACACTGG AGTTTTATGC ACTTGTTTCT CAGGAACTAC AGAGAGCTGA CTTGTGTCTC 
TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAC 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCTAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCTCTTGGC CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG 
GAAACTTCAT TGACATCTCA TGATTTATTT GACATTGATC CTGTTGTAGC CAGATCAGTA 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTGG AGCAGGATAA ATCCCAGACC 
AAAGAGAGTC TACAATATGC ATTGGAAACT CTTACGATGA ATGGCTGTTC AGTGGAAGAT 
CTGGGATTGG ATTTTACGCT GCCTGGGTTT CCCAACATTG AATTGAAGAA AGGAGGGAAG 
GATATTCCAG TTACTATCCA CAATTTGGAA GAGTACCTGA GATTGGTGAT ATTCTGGGCA 
CTAAATGAAG GTGTTTGTCG ACAGTTTGAC TCATTCAGAG ATGGATTTGA ATCGGTCTTC 
CCGCTCTGCC ATCTTCAGTA CTTCTACCCA GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACATTG ATGGAATGCT GCAGGCCTGA TCATGGCTAT 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TGTTTCTCCA GTTTGTGACT GGTAGCCCAC GATTGCCAGT TGGAGGATTT 
CGGAGTTTAA ATCCACCTCT GACAATTGTG CGGAAGACAT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TACCATCAGT AATGACTTGT GTGAACTATC TTAAATTGCC GGACTACTCG 
AGCATTGACA TAATGCGGGA CAAACTCTTG ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249293.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105153.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X44, mRNA.

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CloneID	OMu85020
Clone ID Related Accession (Same CDS sequence)	XM_030249293.1
Accession Version	XM_030249293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCTCTGT TAGAGGCCAG GGGTCTTCCT CCTCACCTAT TTGGTCCTCT CGGGCCTCGG 
ATGTCACAAC TTTTCCATAG AACAATTGGA AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT 
CTTCAAGGAC TACAAGCCAG TGATGAAAGT CAACAGCTTC AGGCCGTCAT TGAAATGTGT 
CAGTTATTGG TCATGGGGAA TGAGGAGACA CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT 
CCAGCTTTGA TAACATTACT GCAGATGGAA CATAATTTTG ACATTATGAA TCATGCTTGC 
CGAGCCTTAA CATATATGAT GGAAGCTCTC CCCCGGTCAT CTGCTGTTGT AGTGGATGCT 
ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT ATTCAGTGTA TTGATGTGGC AGAGCAGGCC 
TTGACTGCCT TGGAGATGTT GTCACGGCGA CACAGTAAAG CCATTTTACA AGCGGGTGGT 
TTGGCAGATT GCTTGTTGTA TCTAGAATTC TTCAGCATCA ATGCCCAAAG AAATGCACTA 
GCAATTGCAG CCAATTGCTG CCAGAGTATC ACACCAGATG AGTTTCATTT TGTGGCAGAT 
TCCCTACCAT TGCTTACCCA AAGGCTAACT CATCAGGACA AAAAGTCAGT AGAAAGTACT 
TGCCTTTGTT TTGCACGTTT AGTGGACAAC TTTCAACATG AGGAGAACTT ACTGCAACAG 
GTTGCTTCCA AAGATCTACT TACAAATGTT CAACAATTGT TGGTAGTGAC TCCACCGATT 
TTAAGTTCTG GGATGTTTAT TATGGTGGTT CGCATGTTTT CTCTGATGTG TTCCAACTGT 
CCAACATTAG CTGTTCAACT TATGAAACAA AACATTGCAG AAACACTTCA CTTCCTGCTA 
TGTGGTGCCT CCAATGGAAG TTGTCAAGAG CAGATTGACC TTGTTCCACG AAGTCCTCAA 
GAGCTGTATG AGCTGACATC TTTGATTTGT GAACTTATGC CATGTTTACC AAAAGAAGGC 
ATCTTCGCAG TTGATACCAT GTTGAAAAAG GGAAATGCAC AGAACACAGA TGGTGCAATA 
TGGCAGTGGC GGGATGACCG AGGCCTCTGG CATCCCTATA ACAGGATTGA CAGCCGGATC 
ATTGAGGTAG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AAGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA TCCCTTAGCC AATTCTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTAATG AAAGAAGATC CGGAACTGGC TAAGTCTTTC 
ATTAAGACCT TGTTTGGTGT TCTCTATGAA GTGTACAGCT CCTCAGCAGG CCCTGCGGTC 
AGACACAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCTGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCAATGCT ATCCAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCTCTTCAG ATGGCAGAAA TCCTAATGCA AAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGGAGAGAA GGTGTGATGC ATCAAGTCAA ACATTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCCCCC AAGGCATGTA CAAATGGGTC AGGATCCCTG 
GGATCTACAA CACCAGCCAG CAGTGGGACT GCAACGGCAG CAACCAACGC CTCTGCTGAT 
CTGGGGTCAC CTAGCTTGCA GCATAGCAGA GATGATTCTT TGGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTCAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA 
TATTCCCCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCCTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA TATCGAACCA GCACGAACTG CAGGAGTTAG TGGTCTTGCC 
AGGGCAGCCT CAAAAGACAC CATATCCAAT AATAGAGAAA AAATTAAAGG CTGGATTAAG 
GAGCAGGCAC ATAAATTTGT TGAGCGTTAT TTTAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCCTTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCT 
TCATTTGAAA TCCAACATAG TGGGTTTGTG AAACAACTGT TGCTGTATTT GACATCTAAG 
AATGAAAAGG ATGCTGTCGG CAGAGAGATC CGGTTAAAAC GTTTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT GGAAGAGTAG AACCAGTGGG CCATGCACCT 
TTGCTGGCAT TGGTTCACAA GATGAACAAC TGTCTCAGCC AAATGGAGCA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGGAACGGA GCTGGAGGAA GCTTTTCTCT CAACAGAGGA 
TCACAAGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GTCAGTTACA AAGACACCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAAGGC GGGCCTGTCA AGATTGATCC CCTGGCTCTG 
GTGCAAGCTA TTGAGAGATA CCTTGTGGTT AGAGGTATTT TCCGATATGG AAGAGTAAGA 
GAAGATGATG AAGATAGTGA TGATGATGGG TCAGATGAAG AAATAGATGA GTCTTTGGCT 
GCTCAATTCT TAAATTCGGG AAATGTAAGG CACAGGCTAC AGTTTTATAT TGGAGAACAT 
TTACTACCAT ATAACATGAC CGTGTATCAG GCAGTGCGGC AATTCAGTGT ACAGGCTGAA 
GATGAAAGAG AATCTACTGA TGATGAGAGC AATCCTCTGG GAAGAGCTGG AATTTGGACA 
AAGACTCATA CAATATGGTA CAAACCTGTG AGGGAGGATG AAGAAAGTAC TAAAGATTGT 
GTTGGTGGCA AAAGGGGGAG AGCCCAAACA GCTCCAACAA AAACTTCCCC CAGAAATGCA 
AAGAAGCATG ATGAGTTATG GCATGATGGA GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA 
GTTTACCTCA TTCCCACACC ACCTGAAAAT ATCACCTTTG AAGACCCATC CTTAGATGTA 
ATACTTCTTT TAAGAGTTTT ACATGCCATC AGTCGATATT GGTATTACTT GTATGATAAT 
GCGATGTGCA AGGAGATTAT TCCAACTAGT GAATTTATTA ACAGTAAATT AACAGCAAAA 
GCAAATAGGC AGCTTCAAGA CCCTCTAGTA ATCATGACAG GAAACATCCC AACATGGCTC 
ACTGAGCTAG GAAAAACCTG CCCATTTTTC TTTCCTTTTG ATACCCGACA AATGCTTTTT 
TATGTAACTG CATTTGATCG GGACCGGGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CTGATTCTCA AGACAGCAGA GTTGCGCCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT ACTGAAACAA GCTGAGTCTG TGATGCAGGA CCTTGGCAGC 
TCTCGGGCCA TGTTAGAAAT CCAATATGAA AATGAGGTTG GTACAGGTCT GGGGCCAACA 
CTGGAGTTTT ATGCACTTGT TTCTCAGGAA CTACAGAGAG CTGACTTGTG TCTCTGGAGG 
GGTGAAGAAG TAACTCTTAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC GCTTCCCTTT GGTAGGACAG CTAAGCCAGC TCACATCGCA 
AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CTAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCTCT TGGCCTGCCC TTTTATAAGT GGATGCTGCG GCAGGAAACT 
TCATTGACAT CTCATGATTT ATTTGACATT GATCCTGTTG TAGCCAGATC AGTATATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTGGAGCAGG ATAAATCCCA GACCAAAGAG 
AGTCTACAAT ATGCATTGGA AACTCTTACG ATGAATGGCT GTTCAGTGGA AGATCTGGGA 
TTGGATTTTA CGCTGCCTGG GTTTCCCAAC ATTGAATTGA AGAAAGGAGG GAAGGATATT 
CCAGTTACTA TCCACAATTT GGAAGAGTAC CTGAGATTGG TGATATTCTG GGCACTAAAT 
GAAGGTGTTT GTCGACAGTT TGACTCATTC AGAGATGGAT TTGAATCGGT CTTCCCGCTC 
TGCCATCTTC AGTACTTCTA CCCAGAAGAG CTGGACCAGC TCCTGTGTGG CAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ATTGATGGAA TGCTGCAGGC CTGATCATGG CTATACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTGTTTC TCCAGTTTGT GACTGGTAGC CCACGATTGC CAGTTGGAGG ATTTCGGAGT 
TTAAATCCAC CTCTGACAAT TGTGCGGAAG ACATTTGAAT CAACAGAAAA CCCAGATGAC 
TTCTTACCAT CAGTAATGAC TTGTGTGAAC TATCTTAAAT TGCCGGACTA CTCGAGCATT 
GACATAATGC GGGACAAACT CTTGATAGCA GCAAGAGAAG GCCAACAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105153.1
CDS	363..6368
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249293.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X44, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249293.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCTCTGT TAGAGGCCAG GGGTCTTCCT CCTCACCTAT TTGGTCCTCT CGGGCCTCGG 
ATGTCACAAC TTTTCCATAG AACAATTGGA AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT 
CTTCAAGGAC TACAAGCCAG TGATGAAAGT CAACAGCTTC AGGCCGTCAT TGAAATGTGT 
CAGTTATTGG TCATGGGGAA TGAGGAGACA CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT 
CCAGCTTTGA TAACATTACT GCAGATGGAA CATAATTTTG ACATTATGAA TCATGCTTGC 
CGAGCCTTAA CATATATGAT GGAAGCTCTC CCCCGGTCAT CTGCTGTTGT AGTGGATGCT 
ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT ATTCAGTGTA TTGATGTGGC AGAGCAGGCC 
TTGACTGCCT TGGAGATGTT GTCACGGCGA CACAGTAAAG CCATTTTACA AGCGGGTGGT 
TTGGCAGATT GCTTGTTGTA TCTAGAATTC TTCAGCATCA ATGCCCAAAG AAATGCACTA 
GCAATTGCAG CCAATTGCTG CCAGAGTATC ACACCAGATG AGTTTCATTT TGTGGCAGAT 
TCCCTACCAT TGCTTACCCA AAGGCTAACT CATCAGGACA AAAAGTCAGT AGAAAGTACT 
TGCCTTTGTT TTGCACGTTT AGTGGACAAC TTTCAACATG AGGAGAACTT ACTGCAACAG 
GTTGCTTCCA AAGATCTACT TACAAATGTT CAACAATTGT TGGTAGTGAC TCCACCGATT 
TTAAGTTCTG GGATGTTTAT TATGGTGGTT CGCATGTTTT CTCTGATGTG TTCCAACTGT 
CCAACATTAG CTGTTCAACT TATGAAACAA AACATTGCAG AAACACTTCA CTTCCTGCTA 
TGTGGTGCCT CCAATGGAAG TTGTCAAGAG CAGATTGACC TTGTTCCACG AAGTCCTCAA 
GAGCTGTATG AGCTGACATC TTTGATTTGT GAACTTATGC CATGTTTACC AAAAGAAGGC 
ATCTTCGCAG TTGATACCAT GTTGAAAAAG GGAAATGCAC AGAACACAGA TGGTGCAATA 
TGGCAGTGGC GGGATGACCG AGGCCTCTGG CATCCCTATA ACAGGATTGA CAGCCGGATC 
ATTGAGGTAG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AAGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA TCCCTTAGCC AATTCTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTAATG AAAGAAGATC CGGAACTGGC TAAGTCTTTC 
ATTAAGACCT TGTTTGGTGT TCTCTATGAA GTGTACAGCT CCTCAGCAGG CCCTGCGGTC 
AGACACAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCTGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCAATGCT ATCCAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCTCTTCAG ATGGCAGAAA TCCTAATGCA AAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGGAGAGAA GGTGTGATGC ATCAAGTCAA ACATTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCCCCC AAGGCATGTA CAAATGGGTC AGGATCCCTG 
GGATCTACAA CACCAGCCAG CAGTGGGACT GCAACGGCAG CAACCAACGC CTCTGCTGAT 
CTGGGGTCAC CTAGCTTGCA GCATAGCAGA GATGATTCTT TGGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTCAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA 
TATTCCCCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCCTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA TATCGAACCA GCACGAACTG CAGGAGTTAG TGGTCTTGCC 
AGGGCAGCCT CAAAAGACAC CATATCCAAT AATAGAGAAA AAATTAAAGG CTGGATTAAG 
GAGCAGGCAC ATAAATTTGT TGAGCGTTAT TTTAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCCTTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCT 
TCATTTGAAA TCCAACATAG TGGGTTTGTG AAACAACTGT TGCTGTATTT GACATCTAAG 
AATGAAAAGG ATGCTGTCGG CAGAGAGATC CGGTTAAAAC GTTTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT GGAAGAGTAG AACCAGTGGG CCATGCACCT 
TTGCTGGCAT TGGTTCACAA GATGAACAAC TGTCTCAGCC AAATGGAGCA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGGAACGGA GCTGGAGGAA GCTTTTCTCT CAACAGAGGA 
TCACAAGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GTCAGTTACA AAGACACCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAAGGC GGGCCTGTCA AGATTGATCC CCTGGCTCTG 
GTGCAAGCTA TTGAGAGATA CCTTGTGGTT AGAGGTATTT TCCGATATGG AAGAGTAAGA 
GAAGATGATG AAGATAGTGA TGATGATGGG TCAGATGAAG AAATAGATGA GTCTTTGGCT 
GCTCAATTCT TAAATTCGGG AAATGTAAGG CACAGGCTAC AGTTTTATAT TGGAGAACAT 
TTACTACCAT ATAACATGAC CGTGTATCAG GCAGTGCGGC AATTCAGTGT ACAGGCTGAA 
GATGAAAGAG AATCTACTGA TGATGAGAGC AATCCTCTGG GAAGAGCTGG AATTTGGACA 
AAGACTCATA CAATATGGTA CAAACCTGTG AGGGAGGATG AAGAAAGTAC TAAAGATTGT 
GTTGGTGGCA AAAGGGGGAG AGCCCAAACA GCTCCAACAA AAACTTCCCC CAGAAATGCA 
AAGAAGCATG ATGAGTTATG GCATGATGGA GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA 
GTTTACCTCA TTCCCACACC ACCTGAAAAT ATCACCTTTG AAGACCCATC CTTAGATGTA 
ATACTTCTTT TAAGAGTTTT ACATGCCATC AGTCGATATT GGTATTACTT GTATGATAAT 
GCGATGTGCA AGGAGATTAT TCCAACTAGT GAATTTATTA ACAGTAAATT AACAGCAAAA 
GCAAATAGGC AGCTTCAAGA CCCTCTAGTA ATCATGACAG GAAACATCCC AACATGGCTC 
ACTGAGCTAG GAAAAACCTG CCCATTTTTC TTTCCTTTTG ATACCCGACA AATGCTTTTT 
TATGTAACTG CATTTGATCG GGACCGGGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CTGATTCTCA AGACAGCAGA GTTGCGCCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT ACTGAAACAA GCTGAGTCTG TGATGCAGGA CCTTGGCAGC 
TCTCGGGCCA TGTTAGAAAT CCAATATGAA AATGAGGTTG GTACAGGTCT GGGGCCAACA 
CTGGAGTTTT ATGCACTTGT TTCTCAGGAA CTACAGAGAG CTGACTTGTG TCTCTGGAGG 
GGTGAAGAAG TAACTCTTAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC GCTTCCCTTT GGTAGGACAG CTAAGCCAGC TCACATCGCA 
AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CTAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCTCT TGGCCTGCCC TTTTATAAGT GGATGCTGCG GCAGGAAACT 
TCATTGACAT CTCATGATTT ATTTGACATT GATCCTGTTG TAGCCAGATC AGTATATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTGGAGCAGG ATAAATCCCA GACCAAAGAG 
AGTCTACAAT ATGCATTGGA AACTCTTACG ATGAATGGCT GTTCAGTGGA AGATCTGGGA 
TTGGATTTTA CGCTGCCTGG GTTTCCCAAC ATTGAATTGA AGAAAGGAGG GAAGGATATT 
CCAGTTACTA TCCACAATTT GGAAGAGTAC CTGAGATTGG TGATATTCTG GGCACTAAAT 
GAAGGTGTTT GTCGACAGTT TGACTCATTC AGAGATGGAT TTGAATCGGT CTTCCCGCTC 
TGCCATCTTC AGTACTTCTA CCCAGAAGAG CTGGACCAGC TCCTGTGTGG CAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ATTGATGGAA TGCTGCAGGC CTGATCATGG CTATACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTGTTTC TCCAGTTTGT GACTGGTAGC CCACGATTGC CAGTTGGAGG ATTTCGGAGT 
TTAAATCCAC CTCTGACAAT TGTGCGGAAG ACATTTGAAT CAACAGAAAA CCCAGATGAC 
TTCTTACCAT CAGTAATGAC TTGTGTGAAC TATCTTAAAT TGCCGGACTA CTCGAGCATT 
GACATAATGC GGGACAAACT CTTGATAGCA GCAAGAGAAG GCCAACAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_030249310.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105170.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X45, mRNA.

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CloneID	OMu85021
Clone ID Related Accession (Same CDS sequence)	XM_030249310.1
Accession Version	XM_030249310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA GACATCAAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAGTCCAC CAAGAAACGC 
AGTGAGTCCC CACCTGCTGA GCTCCCCAGT TTGAGGAGGA GCACACGCCA AAAGACCACG 
GGCTCCTGTG CTAGTACCAG TCGGCGAGGC TCTGGCCTGG GCAAGAGAGG AGCAGCTGAG 
GCCCGGCGGC AAGAGAAAAT GGCAGACCCT GAAAGCAACC AGGAGACAGT AAATTCCTCA 
GCTGCCCGGA CAGATGAAGC TCCCCAAGGA GCTGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTTGGCATGA CCACCTCTGG GGAGAGTGAA TCGGATGATT CTGAGATGGG ACGATTACAA 
GCTCTGTTAG AGGCCAGGGG TCTTCCTCCT CACCTATTTG GTCCTCTCGG GCCTCGGATG 
TCACAACTTT TCCATAGAAC AATTGGAAGC GGAGCTAGTT CTAAGGCCCA GCAGCTTCTT 
CAAGGACTAC AAGCCAGTGA TGAAAGTCAA CAGCTTCAGG CCGTCATTGA AATGTGTCAG 
TTATTGGTCA TGGGGAATGA GGAGACACTA GGAGGGTTTC CTGTGAAGAG TGTTGTTCCA 
GCTTTGATAA CATTACTGCA GATGGAACAT AATTTTGACA TTATGAATCA TGCTTGCCGA 
GCCTTAACAT ATATGATGGA AGCTCTCCCC CGGTCATCTG CTGTTGTAGT GGATGCTATC 
CCTGTTTTCT TAGAAAAGTT GCAAGTTATT CAGTGTATTG ATGTGGCAGA GCAGGCCTTG 
ACTGCCTTGG AGATGTTGTC ACGGCGACAC AGTAAAGCCA TTTTACAAGC GGGTGGTTTG 
GCAGATTGCT TGTTGTATCT AGAATTCTTC AGCATCAATG CCCAAAGAAA TGCACTAGCA 
ATTGCAGCCA ATTGCTGCCA GAGTATCACA CCAGATGAGT TTCATTTTGT GGCAGATTCC 
CTACCATTGC TTACCCAAAG GCTAACTCAT CAGGACAAAA AGTCAGTAGA AAGTACTTGC 
CTTTGTTTTG CACGTTTAGT GGACAACTTT CAACATGAGG AGAACTTACT GCAACAGGTT 
GCTTCCAAAG ATCTACTTAC AAATGTTCAA CAATTGTTGG TAGTGACTCC ACCGATTTTA 
AGTTCTGGGA TGTTTATTAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAACTGTCCA 
ACATTAGCTG TTCAACTTAT GAAACAAAAC ATTGCAGAAA CACTTCACTT CCTGCTATGT 
GGTGCCTCCA ATGGAAGTTG TCAAGAGCAG ATTGACCTTG TTCCACGAAG TCCTCAAGAG 
CTGTATGAGC TGACATCTTT GATTTGTGAA CTTATGCCAT GTTTACCAAA AGAAGGCATC 
TTCGCAGTTG ATACCATGTT GAAAAAGGGA AATGCACAGA ACACAGATGG TGCAATATGG 
CAGTGGCGGG ATGACCGAGG CCTCTGGCAT CCCTATAACA GGATTGACAG CCGGATCATT 
GAGGTAGCAG CCCATCAGGT CGGTGAGGAT GAGATAAGCT TGTCCACTCT GGGACGAGTT 
TATACTATTG ATTTTAATTC TATGCAGCAA ATCAATGAAG ACACGGGAAC AGCACGTGCC 
ATTCAGAGAA AACCTAATCC CTTAGCCAAT TCTAACACTA GTGGATATTC AGAGTTAAAG 
AAGGATGATG CTCGAGCACA GCTAATGAAA GAAGATCCGG AACTGGCTAA GTCTTTCATT 
AAGACCTTGT TTGGTGTTCT CTATGAAGTG TACAGCTCCT CAGCAGGCCC TGCGGTCAGA 
CACAAGTGCC TTAGAGCAAT TCTTAGGATA ATTTATTTTG CTGATGCTGA ACTTCTGAAG 
GATGTTCTGA AAAATCATGC TGTTTCAAGT CACATTGCTT CAATGCTATC CAGCCAAGAC 
CTGAAGATAG TAGTTGGAGC TCTTCAGATG GCAGAAATCC TAATGCAAAA GTTACCTGAT 
ATTTTTAGTG TTTACTTCAG GAGAGAAGGT GTGATGCATC AAGTCAAACA TTTAGCAGAA 
TCAGAGTCTT TGTTGACAAG TCCCCCCAAG GCATGTACAA ATGGGTCAGG ATCCCTGGGA 
TCTACAACAC CAGCCAGCAG TGGGACTGCA ACGGCAGCAA CCAACGCCTC TGCTGATCTG 
GGGTCACCTA GCTTGCAGCA TAGCAGAGAT GATTCTTTGG ATCTGAGCCC TCAAGGTCGA 
TTAAGTGATG TTCTCAAGAG AAAACGACTG CCAAAACGAG GGCCAAGAAG GCCAAAATAT 
TCCCCTCCAA GAGATGATGA CAAAGTAGAC AATCAAGCTA AAAGCCCCAC CACTACTCAG 
TCACCTAAAT CTTCCTTCCT GGCAAGCTTG AATCCAAAAA CATGGGGAAG GTTAAGCGCA 
CAGTCCAACA GCAACAATAT CGAACCAGCA CGAACTGCAG GAGTTAGTGG TCTTGCCAGG 
GCAGCCTCAA AAGACACCAT ATCCAATAAT AGAGAAAAAA TTAAAGGCTG GATTAAGGAG 
CAGGCACATA AATTTGTTGA GCGTTATTTT AGTTCTGAGA ATATGGATGG AAGCAACCCT 
GCCTTGAATG TCCTTCAGAG ACTTTGTGCT GCAACTGAAC AACTCAACCT CCAGGTGGAT 
GGTGGAGCTG AGTGCCTTGT AGAAATCCGT AGCATAGTCT CAGAGTCAGA TGTTTCTTCA 
TTTGAAATCC AACATAGTGG GTTTGTGAAA CAACTGTTGC TGTATTTGAC ATCTAAGAAT 
GAAAAGGATG CTGTCGGCAG AGAGATCCGG TTAAAACGTT TCCTTCATGT ATTTTTTTCT 
TCTCCACTTC CTGGAGAAGA GCCTGTTGGA AGAGTAGAAC CAGTGGGCCA TGCACCTTTG 
CTGGCATTGG TTCACAAGAT GAACAACTGT CTCAGCCAAA TGGAGCAATT TCCAGTCAAA 
GTACATGATT TCCCTAGTGG GAACGGAGCT GGAGGAAGCT TTTCTCTCAA CAGAGGATCA 
CAAGCTTTAA AATTTTTCAA CACACATCAG TTAAAATGTC AGTTACAAAG ACACCCAGAC 
TGTGCAAATG TGAAGCAGTG GAAAGGCGGG CCTGTCAAGA TTGATCCCCT GGCTCTGGTG 
CAAGCTATTG AGAGATACCT TGTGGTTAGA GGTATTTTCC GATATGGAAG AGTAAGAGAA 
GATGATGAAG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAGTC TTTGGCTGCT 
CAATTCTTAA ATTCGGGAAA TGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTA 
CTACCATATA ACATGACCGT GTATCAGGCA GTGCGGCAAT TCAGTGTACA GGCTGAAGAT 
GAAAGAGAAT CTACTGATGA TGAGAGCAAT CCTCTGGGAA GAGCTGGAAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGG GAGGATGAAG AAAGTACTAA AGATTGTGTT 
GGTGGCAAAA GGGGGAGAGC CCAAACAGCT CCAACAAAAA CTTCCCCCAG AAATGCAAAG 
AAGCATGATG AGTTATGGCA TGATGGAGTG TGCCCGTCAG TAGCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATC ACCTTTGAAG ACCCATCCTT AGATGTAATA 
CTTCTTTTAA GAGTTTTACA TGCCATCAGT CGATATTGGT ATTACTTGTA TGATAATGCG 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAATTAAC AGCAAAAGCA 
AATAGGCAGC TTCAAGACCC TCTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTCACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGACAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGGGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA CAGCAGAGTT GCGCCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTACT GAAACAAGCT GAGTCTGTGA TGCAGGACCT TGGCAGCTCT 
CGGGCCATGT TAGAAATCCA ATATGAAAAT GAGGTTGGTA CAGGTCTGGG GCCAACACTG 
GAGTTTTATG CACTTGTTTC TCAGGAACTA CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA CATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCTA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCTCTTGG CCTGCCCTTT TATAAGTGGA TGCTGCGGCA GGAAACTTCA 
TTGACATCTC ATGATTTATT TGACATTGAT CCTGTTGTAG CCAGATCAGT ATATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTG GAGCAGGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTGGAAAC TCTTACGATG AATGGCTGTT CAGTGGAAGA TCTGGGATTG 
GATTTTACGC TGCCTGGGTT TCCCAACATT GAATTGAAGA AAGGAGGGAA GGATATTCCA 
GTTACTATCC ACAATTTGGA AGAGTACCTG AGATTGGTGA TATTCTGGGC ACTAAATGAA 
GGTGTTTGTC GACAGTTTGA CTCATTCAGA GATGGATTTG AATCGGTCTT CCCGCTCTGC 
CATCTTCAGT ACTTCTACCC AGAAGAGCTG GACCAGCTCC TGTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACATT GATGGAATGC TGCAGGCCTG ATCATGGCTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA CGATTGCCAG TTGGAGGATT TCGGAGTTTA 
AATCCACCTC TGACAATTGT GCGGAAGACA TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTACCATCAG TAATGACTTG TGTGAACTAT CTTAAATTGC CGGACTACTC GAGCATTGAC 
ATAATGCGGG ACAAACTCTT GATAGCAGCA AGAGAAGGCC AACAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105170.1
CDS	404..6406
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_030249310.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X45, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249310.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA GACATCAAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAGTCCAC CAAGAAACGC 
AGTGAGTCCC CACCTGCTGA GCTCCCCAGT TTGAGGAGGA GCACACGCCA AAAGACCACG 
GGCTCCTGTG CTAGTACCAG TCGGCGAGGC TCTGGCCTGG GCAAGAGAGG AGCAGCTGAG 
GCCCGGCGGC AAGAGAAAAT GGCAGACCCT GAAAGCAACC AGGAGACAGT AAATTCCTCA 
GCTGCCCGGA CAGATGAAGC TCCCCAAGGA GCTGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTTGGCATGA CCACCTCTGG GGAGAGTGAA TCGGATGATT CTGAGATGGG ACGATTACAA 
GCTCTGTTAG AGGCCAGGGG TCTTCCTCCT CACCTATTTG GTCCTCTCGG GCCTCGGATG 
TCACAACTTT TCCATAGAAC AATTGGAAGC GGAGCTAGTT CTAAGGCCCA GCAGCTTCTT 
CAAGGACTAC AAGCCAGTGA TGAAAGTCAA CAGCTTCAGG CCGTCATTGA AATGTGTCAG 
TTATTGGTCA TGGGGAATGA GGAGACACTA GGAGGGTTTC CTGTGAAGAG TGTTGTTCCA 
GCTTTGATAA CATTACTGCA GATGGAACAT AATTTTGACA TTATGAATCA TGCTTGCCGA 
GCCTTAACAT ATATGATGGA AGCTCTCCCC CGGTCATCTG CTGTTGTAGT GGATGCTATC 
CCTGTTTTCT TAGAAAAGTT GCAAGTTATT CAGTGTATTG ATGTGGCAGA GCAGGCCTTG 
ACTGCCTTGG AGATGTTGTC ACGGCGACAC AGTAAAGCCA TTTTACAAGC GGGTGGTTTG 
GCAGATTGCT TGTTGTATCT AGAATTCTTC AGCATCAATG CCCAAAGAAA TGCACTAGCA 
ATTGCAGCCA ATTGCTGCCA GAGTATCACA CCAGATGAGT TTCATTTTGT GGCAGATTCC 
CTACCATTGC TTACCCAAAG GCTAACTCAT CAGGACAAAA AGTCAGTAGA AAGTACTTGC 
CTTTGTTTTG CACGTTTAGT GGACAACTTT CAACATGAGG AGAACTTACT GCAACAGGTT 
GCTTCCAAAG ATCTACTTAC AAATGTTCAA CAATTGTTGG TAGTGACTCC ACCGATTTTA 
AGTTCTGGGA TGTTTATTAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAACTGTCCA 
ACATTAGCTG TTCAACTTAT GAAACAAAAC ATTGCAGAAA CACTTCACTT CCTGCTATGT 
GGTGCCTCCA ATGGAAGTTG TCAAGAGCAG ATTGACCTTG TTCCACGAAG TCCTCAAGAG 
CTGTATGAGC TGACATCTTT GATTTGTGAA CTTATGCCAT GTTTACCAAA AGAAGGCATC 
TTCGCAGTTG ATACCATGTT GAAAAAGGGA AATGCACAGA ACACAGATGG TGCAATATGG 
CAGTGGCGGG ATGACCGAGG CCTCTGGCAT CCCTATAACA GGATTGACAG CCGGATCATT 
GAGGTAGCAG CCCATCAGGT CGGTGAGGAT GAGATAAGCT TGTCCACTCT GGGACGAGTT 
TATACTATTG ATTTTAATTC TATGCAGCAA ATCAATGAAG ACACGGGAAC AGCACGTGCC 
ATTCAGAGAA AACCTAATCC CTTAGCCAAT TCTAACACTA GTGGATATTC AGAGTTAAAG 
AAGGATGATG CTCGAGCACA GCTAATGAAA GAAGATCCGG AACTGGCTAA GTCTTTCATT 
AAGACCTTGT TTGGTGTTCT CTATGAAGTG TACAGCTCCT CAGCAGGCCC TGCGGTCAGA 
CACAAGTGCC TTAGAGCAAT TCTTAGGATA ATTTATTTTG CTGATGCTGA ACTTCTGAAG 
GATGTTCTGA AAAATCATGC TGTTTCAAGT CACATTGCTT CAATGCTATC CAGCCAAGAC 
CTGAAGATAG TAGTTGGAGC TCTTCAGATG GCAGAAATCC TAATGCAAAA GTTACCTGAT 
ATTTTTAGTG TTTACTTCAG GAGAGAAGGT GTGATGCATC AAGTCAAACA TTTAGCAGAA 
TCAGAGTCTT TGTTGACAAG TCCCCCCAAG GCATGTACAA ATGGGTCAGG ATCCCTGGGA 
TCTACAACAC CAGCCAGCAG TGGGACTGCA ACGGCAGCAA CCAACGCCTC TGCTGATCTG 
GGGTCACCTA GCTTGCAGCA TAGCAGAGAT GATTCTTTGG ATCTGAGCCC TCAAGGTCGA 
TTAAGTGATG TTCTCAAGAG AAAACGACTG CCAAAACGAG GGCCAAGAAG GCCAAAATAT 
TCCCCTCCAA GAGATGATGA CAAAGTAGAC AATCAAGCTA AAAGCCCCAC CACTACTCAG 
TCACCTAAAT CTTCCTTCCT GGCAAGCTTG AATCCAAAAA CATGGGGAAG GTTAAGCGCA 
CAGTCCAACA GCAACAATAT CGAACCAGCA CGAACTGCAG GAGTTAGTGG TCTTGCCAGG 
GCAGCCTCAA AAGACACCAT ATCCAATAAT AGAGAAAAAA TTAAAGGCTG GATTAAGGAG 
CAGGCACATA AATTTGTTGA GCGTTATTTT AGTTCTGAGA ATATGGATGG AAGCAACCCT 
GCCTTGAATG TCCTTCAGAG ACTTTGTGCT GCAACTGAAC AACTCAACCT CCAGGTGGAT 
GGTGGAGCTG AGTGCCTTGT AGAAATCCGT AGCATAGTCT CAGAGTCAGA TGTTTCTTCA 
TTTGAAATCC AACATAGTGG GTTTGTGAAA CAACTGTTGC TGTATTTGAC ATCTAAGAAT 
GAAAAGGATG CTGTCGGCAG AGAGATCCGG TTAAAACGTT TCCTTCATGT ATTTTTTTCT 
TCTCCACTTC CTGGAGAAGA GCCTGTTGGA AGAGTAGAAC CAGTGGGCCA TGCACCTTTG 
CTGGCATTGG TTCACAAGAT GAACAACTGT CTCAGCCAAA TGGAGCAATT TCCAGTCAAA 
GTACATGATT TCCCTAGTGG GAACGGAGCT GGAGGAAGCT TTTCTCTCAA CAGAGGATCA 
CAAGCTTTAA AATTTTTCAA CACACATCAG TTAAAATGTC AGTTACAAAG ACACCCAGAC 
TGTGCAAATG TGAAGCAGTG GAAAGGCGGG CCTGTCAAGA TTGATCCCCT GGCTCTGGTG 
CAAGCTATTG AGAGATACCT TGTGGTTAGA GGTATTTTCC GATATGGAAG AGTAAGAGAA 
GATGATGAAG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAGTC TTTGGCTGCT 
CAATTCTTAA ATTCGGGAAA TGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTA 
CTACCATATA ACATGACCGT GTATCAGGCA GTGCGGCAAT TCAGTGTACA GGCTGAAGAT 
GAAAGAGAAT CTACTGATGA TGAGAGCAAT CCTCTGGGAA GAGCTGGAAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGG GAGGATGAAG AAAGTACTAA AGATTGTGTT 
GGTGGCAAAA GGGGGAGAGC CCAAACAGCT CCAACAAAAA CTTCCCCCAG AAATGCAAAG 
AAGCATGATG AGTTATGGCA TGATGGAGTG TGCCCGTCAG TAGCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATC ACCTTTGAAG ACCCATCCTT AGATGTAATA 
CTTCTTTTAA GAGTTTTACA TGCCATCAGT CGATATTGGT ATTACTTGTA TGATAATGCG 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAATTAAC AGCAAAAGCA 
AATAGGCAGC TTCAAGACCC TCTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTCACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGACAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGGGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA CAGCAGAGTT GCGCCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTACT GAAACAAGCT GAGTCTGTGA TGCAGGACCT TGGCAGCTCT 
CGGGCCATGT TAGAAATCCA ATATGAAAAT GAGGTTGGTA CAGGTCTGGG GCCAACACTG 
GAGTTTTATG CACTTGTTTC TCAGGAACTA CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA CATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCTA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCTCTTGG CCTGCCCTTT TATAAGTGGA TGCTGCGGCA GGAAACTTCA 
TTGACATCTC ATGATTTATT TGACATTGAT CCTGTTGTAG CCAGATCAGT ATATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTG GAGCAGGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTGGAAAC TCTTACGATG AATGGCTGTT CAGTGGAAGA TCTGGGATTG 
GATTTTACGC TGCCTGGGTT TCCCAACATT GAATTGAAGA AAGGAGGGAA GGATATTCCA 
GTTACTATCC ACAATTTGGA AGAGTACCTG AGATTGGTGA TATTCTGGGC ACTAAATGAA 
GGTGTTTGTC GACAGTTTGA CTCATTCAGA GATGGATTTG AATCGGTCTT CCCGCTCTGC 
CATCTTCAGT ACTTCTACCC AGAAGAGCTG GACCAGCTCC TGTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACATT GATGGAATGC TGCAGGCCTG ATCATGGCTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA CGATTGCCAG TTGGAGGATT TCGGAGTTTA 
AATCCACCTC TGACAATTGT GCGGAAGACA TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTACCATCAG TAATGACTTG TGTGAACTAT CTTAAATTGC CGGACTACTC GAGCATTGAC 
ATAATGCGGG ACAAACTCTT GATAGCAGCA AGAGAAGGCC AACAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( NM_133975.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598736.4 Mus musculus thyroid hormone receptor interactor 12 (Trip12), mRNA.

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CloneID	OMu15102
Clone ID Related Accession (Same CDS sequence)	NM_133975.4
Accession Version	NM_133975.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158807.1, AW543112.1, CF747879.1, CN536706.1, CA319321.1, DV647354.1, BG695625.1 and AC123882.6. On Apr 12, 2006 this sequence version replaced NM_133975.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAAGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ATCCCTTAGC CAATTCTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAT 
CCGGAACTGG CTAAGTCTTT CATTAAGACC TTGTTTGGTG TTCTCTATGA AGTGTACAGC 
TCCTCAGCAG GCCCTGCGGT CAGACACAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCTGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCAATGC TATCCAGCCA AGACCTGAAG ATAGTAGTTG GAGCTCTTCA GATGGCAGAA 
ATCCTAATGC AAAAGTTACC TGATATTTTT AGTGTTTACT TCAGGAGAGA AGGTGTGATG 
CATCAAGTCA AACATTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCCCC CAAGGCATGT 
ACAAATGGGT CAGGATCCCT GGGATCTACA ACACCAGCCA GCAGTGGGAC TGCAACGGCA 
GCAACCAACG CCTCTGCTGA TCTGGGGTCA CCTAGCTTGC AGCATAGCAG AGATGATTCT 
TTGGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTCA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA ATATTCCCCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCCT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ATATCGAACC AGCACGAACT 
GCAGGAGTTA GTGGTCTTGC CAGGGCAGCC TCAAAAGACA CCATATCCAA TAATAGAGAA 
AAAATTAAAG GCTGGATTAA GGAGCAGGCA CATAAATTTG TTGAGCGTTA TTTTAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCCTTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC TTCATTTGAA ATCCAACATA GTGGGTTTGT GAAACAACTG 
TTGCTGTATT TGACATCTAA GAATGAAAAG GATGCTGTCG GCAGAGAGAT CCGGTTAAAA 
CGTTTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCTGT TGGAAGAGTA 
GAACCAGTGG GCCATGCACC TTTGCTGGCA TTGGTTCACA AGATGAACAA CTGTCTCAGC 
CAAATGGAGC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGGAACGG AGCTGGAGGA 
AGCTTTTCTC TCAACAGAGG ATCACAAGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGTCAGTTAC AAAGACACCC AGACTGTGCA AATGTGAAGC AGTGGAAAGG CGGGCCTGTC 
AAGATTGATC CCCTGGCTCT GGTGCAAGCT ATTGAGAGAT ACCTTGTGGT TAGAGGATAT 
GGAAGAGTAA GAGAAGATGA TGAAGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAGTCTTTGG CTGCTCAATT CTTAAATTCG GGAAATGTAA GGCACAGGCT ACAGTTTTAT 
ATTGGAGAAC ATTTACTACC ATATAACATG ACCGTGTATC AGGCAGTGCG GCAATTCAGT 
GTACAGGCTG AAGATGAAAG AGAATCTACT GATGATGAGA GCAATCCTCT GGGAAGAGCT 
GGAATTTGGA CAAAGACTCA TACAATATGG TACAAACCTG TGAGGGAGGA TGAAGAAAGT 
ACTAAAGATT GTGTTGGTGG CAAAAGGGGG AGAGCCCAAA CAGCTCCAAC AAAAACTTCC 
CCCAGAAATG CAAAGAAGCA TGATGAGTTA TGGCATGATG GAGTGTGCCC GTCAGTAGCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATCACCTT TGAAGACCCA 
TCCTTAGATG TAATACTTCT TTTAAGAGTT TTACATGCCA TCAGTCGATA TTGGTATTAC 
TTGTATGATA ATGCGATGTG CAAGGAGATT ATTCCAACTA GTGAATTTAT TAACAGTAAA 
TTAACAGCAA AAGCAAATAG GCAGCTTCAA GACCCTCTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TCACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGA 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGGG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGACAGCA GAGTTGCGCC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTACTGAAAC AAGCTGAGTC TGTGATGCAG 
GACCTTGGCA GCTCTCGGGC CATGTTAGAA ATCCAATATG AAAATGAGGT TGGTACAGGT 
CTGGGGCCAA CACTGGAGTT TTATGCACTT GTTTCTCAGG AACTACAGAG AGCTGACTTG 
TGTCTCTGGA GGGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCACATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCTAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCT CTTGGCCTGC CCTTTTATAA GTGGATGCTG 
CGGCAGGAAA CTTCATTGAC ATCTCATGAT TTATTTGACA TTGATCCTGT TGTAGCCAGA 
TCAGTATATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTGGAGCA GGATAAATCC 
CAGACCAAAG AGAGTCTACA ATATGCATTG GAAACTCTTA CGATGAATGG CTGTTCAGTG 
GAAGATCTGG GATTGGATTT TACGCTGCCT GGGTTTCCCA ACATTGAATT GAAGAAAGGA 
GGGAAGGATA TTCCAGTTAC TATCCACAAT TTGGAAGAGT ACCTGAGATT GGTGATATTC 
TGGGCACTAA ATGAAGGTGT TTGTCGACAG TTTGACTCAT TCAGAGATGG ATTTGAATCG 
GTCTTCCCGC TCTGCCATCT TCAGTACTTC TACCCAGAAG AGCTGGACCA GCTCCTGTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACATTGATGG AATGCTGCAG GCCTGATCAT 
GGCTATACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTGTT TCTCCAGTTT GTGACTGGTA GCCCACGATT GCCAGTTGGA 
GGATTTCGGA GTTTAAATCC ACCTCTGACA ATTGTGCGGA AGACATTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTACC ATCAGTAATG ACTTGTGTGA ACTATCTTAA ATTGCCGGAC 
TACTCGAGCA TTGACATAAT GCGGGACAAA CTCTTGATAG CAGCAAGAGA AGGCCAACAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598736.4
CDS	126..6203
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_133975.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133975.4

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAAGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ATCCCTTAGC CAATTCTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAT 
CCGGAACTGG CTAAGTCTTT CATTAAGACC TTGTTTGGTG TTCTCTATGA AGTGTACAGC 
TCCTCAGCAG GCCCTGCGGT CAGACACAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCTGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCAATGC TATCCAGCCA AGACCTGAAG ATAGTAGTTG GAGCTCTTCA GATGGCAGAA 
ATCCTAATGC AAAAGTTACC TGATATTTTT AGTGTTTACT TCAGGAGAGA AGGTGTGATG 
CATCAAGTCA AACATTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCCCC CAAGGCATGT 
ACAAATGGGT CAGGATCCCT GGGATCTACA ACACCAGCCA GCAGTGGGAC TGCAACGGCA 
GCAACCAACG CCTCTGCTGA TCTGGGGTCA CCTAGCTTGC AGCATAGCAG AGATGATTCT 
TTGGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTCA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA ATATTCCCCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCCT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ATATCGAACC AGCACGAACT 
GCAGGAGTTA GTGGTCTTGC CAGGGCAGCC TCAAAAGACA CCATATCCAA TAATAGAGAA 
AAAATTAAAG GCTGGATTAA GGAGCAGGCA CATAAATTTG TTGAGCGTTA TTTTAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCCTTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC TTCATTTGAA ATCCAACATA GTGGGTTTGT GAAACAACTG 
TTGCTGTATT TGACATCTAA GAATGAAAAG GATGCTGTCG GCAGAGAGAT CCGGTTAAAA 
CGTTTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCTGT TGGAAGAGTA 
GAACCAGTGG GCCATGCACC TTTGCTGGCA TTGGTTCACA AGATGAACAA CTGTCTCAGC 
CAAATGGAGC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGGAACGG AGCTGGAGGA 
AGCTTTTCTC TCAACAGAGG ATCACAAGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGTCAGTTAC AAAGACACCC AGACTGTGCA AATGTGAAGC AGTGGAAAGG CGGGCCTGTC 
AAGATTGATC CCCTGGCTCT GGTGCAAGCT ATTGAGAGAT ACCTTGTGGT TAGAGGATAT 
GGAAGAGTAA GAGAAGATGA TGAAGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAGTCTTTGG CTGCTCAATT CTTAAATTCG GGAAATGTAA GGCACAGGCT ACAGTTTTAT 
ATTGGAGAAC ATTTACTACC ATATAACATG ACCGTGTATC AGGCAGTGCG GCAATTCAGT 
GTACAGGCTG AAGATGAAAG AGAATCTACT GATGATGAGA GCAATCCTCT GGGAAGAGCT 
GGAATTTGGA CAAAGACTCA TACAATATGG TACAAACCTG TGAGGGAGGA TGAAGAAAGT 
ACTAAAGATT GTGTTGGTGG CAAAAGGGGG AGAGCCCAAA CAGCTCCAAC AAAAACTTCC 
CCCAGAAATG CAAAGAAGCA TGATGAGTTA TGGCATGATG GAGTGTGCCC GTCAGTAGCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATCACCTT TGAAGACCCA 
TCCTTAGATG TAATACTTCT TTTAAGAGTT TTACATGCCA TCAGTCGATA TTGGTATTAC 
TTGTATGATA ATGCGATGTG CAAGGAGATT ATTCCAACTA GTGAATTTAT TAACAGTAAA 
TTAACAGCAA AAGCAAATAG GCAGCTTCAA GACCCTCTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TCACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGA 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGGG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGACAGCA GAGTTGCGCC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTACTGAAAC AAGCTGAGTC TGTGATGCAG 
GACCTTGGCA GCTCTCGGGC CATGTTAGAA ATCCAATATG AAAATGAGGT TGGTACAGGT 
CTGGGGCCAA CACTGGAGTT TTATGCACTT GTTTCTCAGG AACTACAGAG AGCTGACTTG 
TGTCTCTGGA GGGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCACATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCTAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCT CTTGGCCTGC CCTTTTATAA GTGGATGCTG 
CGGCAGGAAA CTTCATTGAC ATCTCATGAT TTATTTGACA TTGATCCTGT TGTAGCCAGA 
TCAGTATATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTGGAGCA GGATAAATCC 
CAGACCAAAG AGAGTCTACA ATATGCATTG GAAACTCTTA CGATGAATGG CTGTTCAGTG 
GAAGATCTGG GATTGGATTT TACGCTGCCT GGGTTTCCCA ACATTGAATT GAAGAAAGGA 
GGGAAGGATA TTCCAGTTAC TATCCACAAT TTGGAAGAGT ACCTGAGATT GGTGATATTC 
TGGGCACTAA ATGAAGGTGT TTGTCGACAG TTTGACTCAT TCAGAGATGG ATTTGAATCG 
GTCTTCCCGC TCTGCCATCT TCAGTACTTC TACCCAGAAG AGCTGGACCA GCTCCTGTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACATTGATGG AATGCTGCAG GCCTGATCAT 
GGCTATACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTGTT TCTCCAGTTT GTGACTGGTA GCCCACGATT GCCAGTTGGA 
GGATTTCGGA GTTTAAATCC ACCTCTGACA ATTGTGCGGA AGACATTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTACC ATCAGTAATG ACTTGTGTGA ACTATCTTAA ATTGCCGGAC 
TACTCGAGCA TTGACATAAT GCGGGACAAA CTCTTGATAG CAGCAAGAGA AGGCCAACAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496409.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496472.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X11, mRNA.

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CloneID	OMu27709
Clone ID Related Accession (Same CDS sequence)	XM_006496409.3 , XM_006496409.4
Accession Version	XM_006496409.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496409.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AAGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA TCCCTTAGCC 
AATTCTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTAATG 
AAAGAAGATC CGGAACTGGC TAAGTCTTTC ATTAAGACCT TGTTTGGTGT TCTCTATGAA 
GTGTACAGCT CCTCAGCAGG CCCTGCGGTC AGACACAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCTGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCAATGCT ATCCAGCCAA GACCTGAAGA TAGTAGTTGG AGCTCTTCAG 
ATGGCAGAAA TCCTAATGCA AAAGTTACCT GATATTTTTA GTGTTTACTT CAGGAGAGAA 
GGTGTGATGC ATCAAGTCAA ACATTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCCCCC 
AAGGCATGTA CAAATGGGTC AGGATCCCTG GGATCTACAA CACCAGCCAG CAGTGGGACT 
GCAACGGCAG CAACCAACGC CTCTGCTGAT CTGGGGTCAC CTAGCTTGCA GCATAGCAGA 
GATGATTCTT TGGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTCAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA TATTCCCCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCCTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA TATCGAACCA 
GCACGAACTG CAGGAGTTAG TGGTCTTGCC AGGGCAGCCT CAAAAGACAC CATATCCAAT 
AATAGAGAAA AAATTAAAGG CTGGATTAAG GAGCAGGCAC ATAAATTTGT TGAGCGTTAT 
TTTAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCCTTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCT TCATTTGAAA TCCAACATAG TGGGTTTGTG 
AAACAACTGT TGCTGTATTT GACATCTAAG AATGAAAAGG ATGCTGTCGG CAGAGAGATC 
CGGTTAAAAC GTTTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT 
GGAAGAGTAG AACCAGTGGG CCATGCACCT TTGCTGGCAT TGGTTCACAA GATGAACAAC 
TGTCTCAGCC AAATGGAGCA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGGAACGGA 
GCTGGAGGAA GCTTTTCTCT CAACAGAGGA TCACAAGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GTCAGTTACA AAGACACCCA GACTGTGCAA ATGTGAAGCA GTGGAAAGGC 
GGGCCTGTCA AGATTGATCC CCTGGCTCTG GTGCAAGCTA TTGAGAGATA CCTTGTGGTT 
AGAGGTATTT TCCGATATGG AAGAGTAAGA GAAGATGATG AAGATAGTGA TGATGATGGG 
TCAGATGAAG AAATAGATGA GTCTTTGGCT GCTCAATTCT TAAATTCGGG AAATGTAAGG 
CACAGGCTAC AGTTTTATAT TGGAGAACAT TTACTACCAT ATAACATGAC CGTGTATCAG 
GCAGTGCGGC AATTCAGTGT ACAGGCTGAA GATGAAAGAG AATCTACTGA TGATGAGAGC 
AATCCTCTGG GAAGAGCTGG AATTTGGACA AAGACTCATA CAATATGGTA CAAACCTGTG 
AGGGAGGATG AAGAAAGTAC TAAAGATTGT GTTGGTGGCA AAAGGGGGAG AGCCCAAACA 
GCTCCAACAA AAACTTCCCC CAGAAATGCA AAGAAGCATG ATGAGTTATG GCATGATGGA 
GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATCACCTTTG AAGACCCATC CTTAGATGTA ATACTTCTTT TAAGAGTTTT ACATGCCATC 
AGTCGATATT GGTATTACTT GTATGATAAT GCGATGTGCA AGGAGATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAATT AACAGCAAAA GCAAATAGGC AGCTTCAAGA CCCTCTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTC ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGACA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGGGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGACAGCAGA 
GTTGCGCCTA GATTGGATAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT ACTGAAACAA 
GCTGAGTCTG TGATGCAGGA CCTTGGCAGC TCTCGGGCCA TGTTAGAAAT CCAATATGAA 
AATGAGGTTG GTACAGGTCT GGGGCCAACA CTGGAGTTTT ATGCACTTGT TTCTCAGGAA 
CTACAGAGAG CTGACTTGTG TCTCTGGAGG GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCACATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CTAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCTCT TGGCCTGCCC 
TTTTATAAGT GGATGCTGCG GCAGGAAACT TCATTGACAT CTCATGATTT ATTTGACATT 
GATCCTGTTG TAGCCAGATC AGTATATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTGGAGCAGG ATAAATCCCA GACCAAAGAG AGTCTACAAT ATGCATTGGA AACTCTTACG 
ATGAATGGCT GTTCAGTGGA AGATCTGGGA TTGGATTTTA CGCTGCCTGG GTTTCCCAAC 
ATTGAATTGA AGAAAGGAGG GAAGGATATT CCAGTTACTA TCCACAATTT GGAAGAGTAC 
CTGAGATTGG TGATATTCTG GGCACTAAAT GAAGGTGTTT GTCGACAGTT TGACTCATTC 
AGAGATGGAT TTGAATCGGT CTTCCCGCTC TGCCATCTTC AGTACTTCTA CCCAGAAGAG 
CTGGACCAGC TCCTGTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ATTGATGGAA 
TGCTGCAGGC CTGATCATGG CTATACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTGTTTC TCCAGTTTGT GACTGGTAGC 
CCACGATTGC CAGTTGGAGG ATTTCGGAGT TTAAATCCAC CTCTGACAAT TGTGCGGAAG 
ACATTTGAAT CAACAGAAAA CCCAGATGAC TTCTTACCAT CAGTAATGAC TTGTGTGAAC 
TATCTTAAAT TGCCGGACTA CTCGAGCATT GACATAATGC GGGACAAACT CTTGATAGCA 
GCAAGAGAAG GCCAACAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496472.1
CDS	400..6615
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496409.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496409.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AAGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA TCCCTTAGCC 
AATTCTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTAATG 
AAAGAAGATC CGGAACTGGC TAAGTCTTTC ATTAAGACCT TGTTTGGTGT TCTCTATGAA 
GTGTACAGCT CCTCAGCAGG CCCTGCGGTC AGACACAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCTGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCAATGCT ATCCAGCCAA GACCTGAAGA TAGTAGTTGG AGCTCTTCAG 
ATGGCAGAAA TCCTAATGCA AAAGTTACCT GATATTTTTA GTGTTTACTT CAGGAGAGAA 
GGTGTGATGC ATCAAGTCAA ACATTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCCCCC 
AAGGCATGTA CAAATGGGTC AGGATCCCTG GGATCTACAA CACCAGCCAG CAGTGGGACT 
GCAACGGCAG CAACCAACGC CTCTGCTGAT CTGGGGTCAC CTAGCTTGCA GCATAGCAGA 
GATGATTCTT TGGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTCAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAA TATTCCCCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCCTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA TATCGAACCA 
GCACGAACTG CAGGAGTTAG TGGTCTTGCC AGGGCAGCCT CAAAAGACAC CATATCCAAT 
AATAGAGAAA AAATTAAAGG CTGGATTAAG GAGCAGGCAC ATAAATTTGT TGAGCGTTAT 
TTTAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCCTTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCT TCATTTGAAA TCCAACATAG TGGGTTTGTG 
AAACAACTGT TGCTGTATTT GACATCTAAG AATGAAAAGG ATGCTGTCGG CAGAGAGATC 
CGGTTAAAAC GTTTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCTGTT 
GGAAGAGTAG AACCAGTGGG CCATGCACCT TTGCTGGCAT TGGTTCACAA GATGAACAAC 
TGTCTCAGCC AAATGGAGCA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGGAACGGA 
GCTGGAGGAA GCTTTTCTCT CAACAGAGGA TCACAAGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GTCAGTTACA AAGACACCCA GACTGTGCAA ATGTGAAGCA GTGGAAAGGC 
GGGCCTGTCA AGATTGATCC CCTGGCTCTG GTGCAAGCTA TTGAGAGATA CCTTGTGGTT 
AGAGGTATTT TCCGATATGG AAGAGTAAGA GAAGATGATG AAGATAGTGA TGATGATGGG 
TCAGATGAAG AAATAGATGA GTCTTTGGCT GCTCAATTCT TAAATTCGGG AAATGTAAGG 
CACAGGCTAC AGTTTTATAT TGGAGAACAT TTACTACCAT ATAACATGAC CGTGTATCAG 
GCAGTGCGGC AATTCAGTGT ACAGGCTGAA GATGAAAGAG AATCTACTGA TGATGAGAGC 
AATCCTCTGG GAAGAGCTGG AATTTGGACA AAGACTCATA CAATATGGTA CAAACCTGTG 
AGGGAGGATG AAGAAAGTAC TAAAGATTGT GTTGGTGGCA AAAGGGGGAG AGCCCAAACA 
GCTCCAACAA AAACTTCCCC CAGAAATGCA AAGAAGCATG ATGAGTTATG GCATGATGGA 
GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATCACCTTTG AAGACCCATC CTTAGATGTA ATACTTCTTT TAAGAGTTTT ACATGCCATC 
AGTCGATATT GGTATTACTT GTATGATAAT GCGATGTGCA AGGAGATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAATT AACAGCAAAA GCAAATAGGC AGCTTCAAGA CCCTCTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTC ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGACA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGGGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGACAGCAGA 
GTTGCGCCTA GATTGGATAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT ACTGAAACAA 
GCTGAGTCTG TGATGCAGGA CCTTGGCAGC TCTCGGGCCA TGTTAGAAAT CCAATATGAA 
AATGAGGTTG GTACAGGTCT GGGGCCAACA CTGGAGTTTT ATGCACTTGT TTCTCAGGAA 
CTACAGAGAG CTGACTTGTG TCTCTGGAGG GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCACATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CTAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCTCT TGGCCTGCCC 
TTTTATAAGT GGATGCTGCG GCAGGAAACT TCATTGACAT CTCATGATTT ATTTGACATT 
GATCCTGTTG TAGCCAGATC AGTATATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTGGAGCAGG ATAAATCCCA GACCAAAGAG AGTCTACAAT ATGCATTGGA AACTCTTACG 
ATGAATGGCT GTTCAGTGGA AGATCTGGGA TTGGATTTTA CGCTGCCTGG GTTTCCCAAC 
ATTGAATTGA AGAAAGGAGG GAAGGATATT CCAGTTACTA TCCACAATTT GGAAGAGTAC 
CTGAGATTGG TGATATTCTG GGCACTAAAT GAAGGTGTTT GTCGACAGTT TGACTCATTC 
AGAGATGGAT TTGAATCGGT CTTCCCGCTC TGCCATCTTC AGTACTTCTA CCCAGAAGAG 
CTGGACCAGC TCCTGTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ATTGATGGAA 
TGCTGCAGGC CTGATCATGG CTATACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTGTTTC TCCAGTTTGT GACTGGTAGC 
CCACGATTGC CAGTTGGAGG ATTTCGGAGT TTAAATCCAC CTCTGACAAT TGTGCGGAAG 
ACATTTGAAT CAACAGAAAA CCCAGATGAC TTCTTACCAT CAGTAATGAC TTGTGTGAAC 
TATCTTAAAT TGCCGGACTA CTCGAGCATT GACATAATGC GGGACAAACT CTTGATAGCA 
GCAAGAGAAG GCCAACAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496414.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496477.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X12, mRNA.

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CloneID	OMu27710
Clone ID Related Accession (Same CDS sequence)	XM_006496414.3 , XM_006496414.4
Accession Version	XM_006496414.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496414.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAAG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAATCC CTTAGCCAAT 
TCTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTAATGAAA 
GAAGATCCGG AACTGGCTAA GTCTTTCATT AAGACCTTGT TTGGTGTTCT CTATGAAGTG 
TACAGCTCCT CAGCAGGCCC TGCGGTCAGA CACAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CTGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CAATGCTATC CAGCCAAGAC CTGAAGATAG TAGTTGGAGC TCTTCAGATG 
GCAGAAATCC TAATGCAAAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG GAGAGAAGGT 
GTGATGCATC AAGTCAAACA TTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCCCCCAAG 
GCATGTACAA ATGGGTCAGG ATCCCTGGGA TCTACAACAC CAGCCAGCAG TGGGACTGCA 
ACGGCAGCAA CCAACGCCTC TGCTGATCTG GGGTCACCTA GCTTGCAGCA TAGCAGAGAT 
GATTCTTTGG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTCAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAATAT TCCCCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCCTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAATAT CGAACCAGCA 
CGAACTGCAG GAGTTAGTGG TCTTGCCAGG GCAGCCTCAA AAGACACCAT ATCCAATAAT 
AGAGAAAAAA TTAAAGGCTG GATTAAGGAG CAGGCACATA AATTTGTTGA GCGTTATTTT 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCCTTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCTTCA TTTGAAATCC AACATAGTGG GTTTGTGAAA 
CAACTGTTGC TGTATTTGAC ATCTAAGAAT GAAAAGGATG CTGTCGGCAG AGAGATCCGG 
TTAAAACGTT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCTGTTGGA 
AGAGTAGAAC CAGTGGGCCA TGCACCTTTG CTGGCATTGG TTCACAAGAT GAACAACTGT 
CTCAGCCAAA TGGAGCAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG GAACGGAGCT 
GGAGGAAGCT TTTCTCTCAA CAGAGGATCA CAAGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGTC AGTTACAAAG ACACCCAGAC TGTGCAAATG TGAAGCAGTG GAAAGGCGGG 
CCTGTCAAGA TTGATCCCCT GGCTCTGGTG CAAGCTATTG AGAGATACCT TGTGGTTAGA 
GGTATTTTCC GATATGGAAG AGTAAGAGAA GATGATGAAG ATAGTGATGA TGATGGGTCA 
GATGAAGAAA TAGATGAGTC TTTGGCTGCT CAATTCTTAA ATTCGGGAAA TGTAAGGCAC 
AGGCTACAGT TTTATATTGG AGAACATTTA CTACCATATA ACATGACCGT GTATCAGGCA 
GTGCGGCAAT TCAGTGTACA GGCTGAAGAT GAAAGAGAAT CTACTGATGA TGAGAGCAAT 
CCTCTGGGAA GAGCTGGAAT TTGGACAAAG ACTCATACAA TATGGTACAA ACCTGTGAGG 
GAGGATGAAG AAAGTACTAA AGATTGTGTT GGTGGCAAAA GGGGGAGAGC CCAAACAGCT 
CCAACAAAAA CTTCCCCCAG AAATGCAAAG AAGCATGATG AGTTATGGCA TGATGGAGTG 
TGCCCGTCAG TAGCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATC 
ACCTTTGAAG ACCCATCCTT AGATGTAATA CTTCTTTTAA GAGTTTTACA TGCCATCAGT 
CGATATTGGT ATTACTTGTA TGATAATGCG ATGTGCAAGG AGATTATTCC AACTAGTGAA 
TTTATTAACA GTAAATTAAC AGCAAAAGCA AATAGGCAGC TTCAAGACCC TCTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTCACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGACAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGGGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA CAGCAGAGTT 
GCGCCTAGAT TGGATAGAAA AAAACGTACT GTGAACAGAG AGGAGCTACT GAAACAAGCT 
GAGTCTGTGA TGCAGGACCT TGGCAGCTCT CGGGCCATGT TAGAAATCCA ATATGAAAAT 
GAGGTTGGTA CAGGTCTGGG GCCAACACTG GAGTTTTATG CACTTGTTTC TCAGGAACTA 
CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCTA AGCCAGCTCA CATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCTA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCTCTTGG CCTGCCCTTT 
TATAAGTGGA TGCTGCGGCA GGAAACTTCA TTGACATCTC ATGATTTATT TGACATTGAT 
CCTGTTGTAG CCAGATCAGT ATATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTG 
GAGCAGGATA AATCCCAGAC CAAAGAGAGT CTACAATATG CATTGGAAAC TCTTACGATG 
AATGGCTGTT CAGTGGAAGA TCTGGGATTG GATTTTACGC TGCCTGGGTT TCCCAACATT 
GAATTGAAGA AAGGAGGGAA GGATATTCCA GTTACTATCC ACAATTTGGA AGAGTACCTG 
AGATTGGTGA TATTCTGGGC ACTAAATGAA GGTGTTTGTC GACAGTTTGA CTCATTCAGA 
GATGGATTTG AATCGGTCTT CCCGCTCTGC CATCTTCAGT ACTTCTACCC AGAAGAGCTG 
GACCAGCTCC TGTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACATT GATGGAATGC 
TGCAGGCCTG ATCATGGCTA TACTCATGAC AGTCGGGCTG TGAAGTTCTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA 
CGATTGCCAG TTGGAGGATT TCGGAGTTTA AATCCACCTC TGACAATTGT GCGGAAGACA 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTACCATCAG TAATGACTTG TGTGAACTAT 
CTTAAATTGC CGGACTACTC GAGCATTGAC ATAATGCGGG ACAAACTCTT GATAGCAGCA 
AGAGAAGGCC AACAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496477.1
CDS	400..6612
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496414.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496414.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAAG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAATCC CTTAGCCAAT 
TCTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTAATGAAA 
GAAGATCCGG AACTGGCTAA GTCTTTCATT AAGACCTTGT TTGGTGTTCT CTATGAAGTG 
TACAGCTCCT CAGCAGGCCC TGCGGTCAGA CACAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CTGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CAATGCTATC CAGCCAAGAC CTGAAGATAG TAGTTGGAGC TCTTCAGATG 
GCAGAAATCC TAATGCAAAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG GAGAGAAGGT 
GTGATGCATC AAGTCAAACA TTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCCCCCAAG 
GCATGTACAA ATGGGTCAGG ATCCCTGGGA TCTACAACAC CAGCCAGCAG TGGGACTGCA 
ACGGCAGCAA CCAACGCCTC TGCTGATCTG GGGTCACCTA GCTTGCAGCA TAGCAGAGAT 
GATTCTTTGG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTCAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAATAT TCCCCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCCTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAATAT CGAACCAGCA 
CGAACTGCAG GAGTTAGTGG TCTTGCCAGG GCAGCCTCAA AAGACACCAT ATCCAATAAT 
AGAGAAAAAA TTAAAGGCTG GATTAAGGAG CAGGCACATA AATTTGTTGA GCGTTATTTT 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCCTTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCTTCA TTTGAAATCC AACATAGTGG GTTTGTGAAA 
CAACTGTTGC TGTATTTGAC ATCTAAGAAT GAAAAGGATG CTGTCGGCAG AGAGATCCGG 
TTAAAACGTT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCTGTTGGA 
AGAGTAGAAC CAGTGGGCCA TGCACCTTTG CTGGCATTGG TTCACAAGAT GAACAACTGT 
CTCAGCCAAA TGGAGCAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG GAACGGAGCT 
GGAGGAAGCT TTTCTCTCAA CAGAGGATCA CAAGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGTC AGTTACAAAG ACACCCAGAC TGTGCAAATG TGAAGCAGTG GAAAGGCGGG 
CCTGTCAAGA TTGATCCCCT GGCTCTGGTG CAAGCTATTG AGAGATACCT TGTGGTTAGA 
GGTATTTTCC GATATGGAAG AGTAAGAGAA GATGATGAAG ATAGTGATGA TGATGGGTCA 
GATGAAGAAA TAGATGAGTC TTTGGCTGCT CAATTCTTAA ATTCGGGAAA TGTAAGGCAC 
AGGCTACAGT TTTATATTGG AGAACATTTA CTACCATATA ACATGACCGT GTATCAGGCA 
GTGCGGCAAT TCAGTGTACA GGCTGAAGAT GAAAGAGAAT CTACTGATGA TGAGAGCAAT 
CCTCTGGGAA GAGCTGGAAT TTGGACAAAG ACTCATACAA TATGGTACAA ACCTGTGAGG 
GAGGATGAAG AAAGTACTAA AGATTGTGTT GGTGGCAAAA GGGGGAGAGC CCAAACAGCT 
CCAACAAAAA CTTCCCCCAG AAATGCAAAG AAGCATGATG AGTTATGGCA TGATGGAGTG 
TGCCCGTCAG TAGCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATC 
ACCTTTGAAG ACCCATCCTT AGATGTAATA CTTCTTTTAA GAGTTTTACA TGCCATCAGT 
CGATATTGGT ATTACTTGTA TGATAATGCG ATGTGCAAGG AGATTATTCC AACTAGTGAA 
TTTATTAACA GTAAATTAAC AGCAAAAGCA AATAGGCAGC TTCAAGACCC TCTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTCACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGACAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGGGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA CAGCAGAGTT 
GCGCCTAGAT TGGATAGAAA AAAACGTACT GTGAACAGAG AGGAGCTACT GAAACAAGCT 
GAGTCTGTGA TGCAGGACCT TGGCAGCTCT CGGGCCATGT TAGAAATCCA ATATGAAAAT 
GAGGTTGGTA CAGGTCTGGG GCCAACACTG GAGTTTTATG CACTTGTTTC TCAGGAACTA 
CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCTA AGCCAGCTCA CATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCTA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCTCTTGG CCTGCCCTTT 
TATAAGTGGA TGCTGCGGCA GGAAACTTCA TTGACATCTC ATGATTTATT TGACATTGAT 
CCTGTTGTAG CCAGATCAGT ATATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTG 
GAGCAGGATA AATCCCAGAC CAAAGAGAGT CTACAATATG CATTGGAAAC TCTTACGATG 
AATGGCTGTT CAGTGGAAGA TCTGGGATTG GATTTTACGC TGCCTGGGTT TCCCAACATT 
GAATTGAAGA AAGGAGGGAA GGATATTCCA GTTACTATCC ACAATTTGGA AGAGTACCTG 
AGATTGGTGA TATTCTGGGC ACTAAATGAA GGTGTTTGTC GACAGTTTGA CTCATTCAGA 
GATGGATTTG AATCGGTCTT CCCGCTCTGC CATCTTCAGT ACTTCTACCC AGAAGAGCTG 
GACCAGCTCC TGTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACATT GATGGAATGC 
TGCAGGCCTG ATCATGGCTA TACTCATGAC AGTCGGGCTG TGAAGTTCTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA 
CGATTGCCAG TTGGAGGATT TCGGAGTTTA AATCCACCTC TGACAATTGT GCGGAAGACA 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTACCATCAG TAATGACTTG TGTGAACTAT 
CTTAAATTGC CGGACTACTC GAGCATTGAC ATAATGCGGG ACAAACTCTT GATAGCAGCA 
AGAGAAGGCC AACAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496416.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496479.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X14, mRNA.

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CloneID	OMu27712
Clone ID Related Accession (Same CDS sequence)	XM_006496416.3 , XM_006496416.4
Accession Version	XM_006496416.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496416.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAAG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAATCC CTTAGCCAAT 
TCTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTAATGAAA 
GAAGATCCGG AACTGGCTAA GTCTTTCATT AAGACCTTGT TTGGTGTTCT CTATGAAGTG 
TACAGCTCCT CAGCAGGCCC TGCGGTCAGA CACAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CTGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CAATGCTATC CAGCCAAGAC CTGAAGATAG TAGTTGGAGC TCTTCAGATG 
GCAGAAATCC TAATGCAAAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG GAGAGAAGGT 
GTGATGCATC AAGTCAAACA TTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCCCCCAAG 
GCATGTACAA ATGGGTCAGG ATCCCTGGGA TCTACAACAC CAGCCAGCAG TGGGACTGCA 
ACGGCAGCAA CCAACGCCTC TGCTGATCTG GGGTCACCTA GCTTGCAGCA TAGCAGAGAT 
GATTCTTTGG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTCAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAATAT TCCCCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCCTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAATAT CGAACCAGCA 
CGAACTGCAG GAGTTAGTGG TCTTGCCAGG GCAGCCTCAA AAGACACCAT ATCCAATAAT 
AGAGAAAAAA TTAAAGGCTG GATTAAGGAG CAGGCACATA AATTTGTTGA GCGTTATTTT 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCCTTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCTTCA TTTGAAATCC AACATAGTGG GTTTGTGAAA 
CAACTGTTGC TGTATTTGAC ATCTAAGAAT GAAAAGGATG CTGTCGGCAG AGAGATCCGG 
TTAAAACGTT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCTGTTGGA 
AGAGTAGAAC CAGTGGGCCA TGCACCTTTG CTGGCATTGG TTCACAAGAT GAACAACTGT 
CTCAGCCAAA TGGAGCAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG GAACGGAGCT 
GGAGGAAGCT TTTCTCTCAA CAGAGGATCA CAAGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGTC AGTTACAAAG ACACCCAGAC TGTGCAAATG TGAAGCAGTG GAAAGGCGGG 
CCTGTCAAGA TTGATCCCCT GGCTCTGGTG CAAGCTATTG AGAGATACCT TGTGGTTAGA 
GGATATGGAA GAGTAAGAGA AGATGATGAA GATAGTGATG ATGATGGGTC AGATGAAGAA 
ATAGATGAGT CTTTGGCTGC TCAATTCTTA AATTCGGGAA ATGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT ACTACCATAT AACATGACCG TGTATCAGGC AGTGCGGCAA 
TTCAGTGTAC AGGCTGAAGA TGAAAGAGAA TCTACTGATG ATGAGAGCAA TCCTCTGGGA 
AGAGCTGGAA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG GGAGGATGAA 
GAAAGTACTA AAGATTGTGT TGGTGGCAAA AGGGGGAGAG CCCAAACAGC TCCAACAAAA 
ACTTCCCCCA GAAATGCAAA GAAGCATGAT GAGTTATGGC ATGATGGAGT GTGCCCGTCA 
GTAGCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT CACCTTTGAA 
GACCCATCCT TAGATGTAAT ACTTCTTTTA AGAGTTTTAC ATGCCATCAG TCGATATTGG 
TATTACTTGT ATGATAATGC GATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAATTAA CAGCAAAAGC AAATAGGCAG CTTCAAGACC CTCTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTCAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGACAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGGGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ACAGCAGAGT TGCGCCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTAC TGAAACAAGC TGAGTCTGTG 
ATGCAGGACC TTGGCAGCTC TCGGGCCATG TTAGAAATCC AATATGAAAA TGAGGTTGGT 
ACAGGTCTGG GGCCAACACT GGAGTTTTAT GCACTTGTTT CTCAGGAACT ACAGAGAGCT 
GACTTGTGTC TCTGGAGGGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ACATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCT 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCTCTTG GCCTGCCCTT TTATAAGTGG 
ATGCTGCGGC AGGAAACTTC ATTGACATCT CATGATTTAT TTGACATTGA TCCTGTTGTA 
GCCAGATCAG TATATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT GGAGCAGGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTGGAAA CTCTTACGAT GAATGGCTGT 
TCAGTGGAAG ATCTGGGATT GGATTTTACG CTGCCTGGGT TTCCCAACAT TGAATTGAAG 
AAAGGAGGGA AGGATATTCC AGTTACTATC CACAATTTGG AAGAGTACCT GAGATTGGTG 
ATATTCTGGG CACTAAATGA AGGTGTTTGT CGACAGTTTG ACTCATTCAG AGATGGATTT 
GAATCGGTCT TCCCGCTCTG CCATCTTCAG TACTTCTACC CAGAAGAGCT GGACCAGCTC 
CTGTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAT TGATGGAATG CTGCAGGCCT 
GATCATGGCT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CTGGTAGCCC ACGATTGCCA 
GTTGGAGGAT TTCGGAGTTT AAATCCACCT CTGACAATTG TGCGGAAGAC ATTTGAATCA 
ACAGAAAACC CAGATGACTT CTTACCATCA GTAATGACTT GTGTGAACTA TCTTAAATTG 
CCGGACTACT CGAGCATTGA CATAATGCGG GACAAACTCT TGATAGCAGC AAGAGAAGGC 
CAACAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496479.1
CDS	400..6603
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496416.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496416.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAAG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAATCC CTTAGCCAAT 
TCTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTAATGAAA 
GAAGATCCGG AACTGGCTAA GTCTTTCATT AAGACCTTGT TTGGTGTTCT CTATGAAGTG 
TACAGCTCCT CAGCAGGCCC TGCGGTCAGA CACAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CTGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CAATGCTATC CAGCCAAGAC CTGAAGATAG TAGTTGGAGC TCTTCAGATG 
GCAGAAATCC TAATGCAAAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG GAGAGAAGGT 
GTGATGCATC AAGTCAAACA TTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCCCCCAAG 
GCATGTACAA ATGGGTCAGG ATCCCTGGGA TCTACAACAC CAGCCAGCAG TGGGACTGCA 
ACGGCAGCAA CCAACGCCTC TGCTGATCTG GGGTCACCTA GCTTGCAGCA TAGCAGAGAT 
GATTCTTTGG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTCAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAATAT TCCCCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCCTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAATAT CGAACCAGCA 
CGAACTGCAG GAGTTAGTGG TCTTGCCAGG GCAGCCTCAA AAGACACCAT ATCCAATAAT 
AGAGAAAAAA TTAAAGGCTG GATTAAGGAG CAGGCACATA AATTTGTTGA GCGTTATTTT 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCCTTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCTTCA TTTGAAATCC AACATAGTGG GTTTGTGAAA 
CAACTGTTGC TGTATTTGAC ATCTAAGAAT GAAAAGGATG CTGTCGGCAG AGAGATCCGG 
TTAAAACGTT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCTGTTGGA 
AGAGTAGAAC CAGTGGGCCA TGCACCTTTG CTGGCATTGG TTCACAAGAT GAACAACTGT 
CTCAGCCAAA TGGAGCAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG GAACGGAGCT 
GGAGGAAGCT TTTCTCTCAA CAGAGGATCA CAAGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGTC AGTTACAAAG ACACCCAGAC TGTGCAAATG TGAAGCAGTG GAAAGGCGGG 
CCTGTCAAGA TTGATCCCCT GGCTCTGGTG CAAGCTATTG AGAGATACCT TGTGGTTAGA 
GGATATGGAA GAGTAAGAGA AGATGATGAA GATAGTGATG ATGATGGGTC AGATGAAGAA 
ATAGATGAGT CTTTGGCTGC TCAATTCTTA AATTCGGGAA ATGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT ACTACCATAT AACATGACCG TGTATCAGGC AGTGCGGCAA 
TTCAGTGTAC AGGCTGAAGA TGAAAGAGAA TCTACTGATG ATGAGAGCAA TCCTCTGGGA 
AGAGCTGGAA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG GGAGGATGAA 
GAAAGTACTA AAGATTGTGT TGGTGGCAAA AGGGGGAGAG CCCAAACAGC TCCAACAAAA 
ACTTCCCCCA GAAATGCAAA GAAGCATGAT GAGTTATGGC ATGATGGAGT GTGCCCGTCA 
GTAGCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT CACCTTTGAA 
GACCCATCCT TAGATGTAAT ACTTCTTTTA AGAGTTTTAC ATGCCATCAG TCGATATTGG 
TATTACTTGT ATGATAATGC GATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAATTAA CAGCAAAAGC AAATAGGCAG CTTCAAGACC CTCTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTCAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGACAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGGGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ACAGCAGAGT TGCGCCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTAC TGAAACAAGC TGAGTCTGTG 
ATGCAGGACC TTGGCAGCTC TCGGGCCATG TTAGAAATCC AATATGAAAA TGAGGTTGGT 
ACAGGTCTGG GGCCAACACT GGAGTTTTAT GCACTTGTTT CTCAGGAACT ACAGAGAGCT 
GACTTGTGTC TCTGGAGGGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ACATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCT 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCTCTTG GCCTGCCCTT TTATAAGTGG 
ATGCTGCGGC AGGAAACTTC ATTGACATCT CATGATTTAT TTGACATTGA TCCTGTTGTA 
GCCAGATCAG TATATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT GGAGCAGGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTGGAAA CTCTTACGAT GAATGGCTGT 
TCAGTGGAAG ATCTGGGATT GGATTTTACG CTGCCTGGGT TTCCCAACAT TGAATTGAAG 
AAAGGAGGGA AGGATATTCC AGTTACTATC CACAATTTGG AAGAGTACCT GAGATTGGTG 
ATATTCTGGG CACTAAATGA AGGTGTTTGT CGACAGTTTG ACTCATTCAG AGATGGATTT 
GAATCGGTCT TCCCGCTCTG CCATCTTCAG TACTTCTACC CAGAAGAGCT GGACCAGCTC 
CTGTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAT TGATGGAATG CTGCAGGCCT 
GATCATGGCT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CTGGTAGCCC ACGATTGCCA 
GTTGGAGGAT TTCGGAGTTT AAATCCACCT CTGACAATTG TGCGGAAGAC ATTTGAATCA 
ACAGAAAACC CAGATGACTT CTTACCATCA GTAATGACTT GTGTGAACTA TCTTAAATTG 
CCGGACTACT CGAGCATTGA CATAATGCGG GACAAACTCT TGATAGCAGC AAGAGAAGGC 
CAACAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496422.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496485.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X20, mRNA.

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CloneID	OMu27718
Clone ID Related Accession (Same CDS sequence)	XM_006496422.3 , XM_006496422.4
Accession Version	XM_006496422.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496422.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAAGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAATCCC TTAGCCAATT CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTAATGAAAG AAGATCCGGA ACTGGCTAAG 
TCTTTCATTA AGACCTTGTT TGGTGTTCTC TATGAAGTGT ACAGCTCCTC AGCAGGCCCT 
GCGGTCAGAC ACAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC TGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC AATGCTATCC 
AGCCAAGACC TGAAGATAGT AGTTGGAGCT CTTCAGATGG CAGAAATCCT AATGCAAAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGG AGAGAAGGTG TGATGCATCA AGTCAAACAT 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCCCCCAAGG CATGTACAAA TGGGTCAGGA 
TCCCTGGGAT CTACAACACC AGCCAGCAGT GGGACTGCAA CGGCAGCAAC CAACGCCTCT 
GCTGATCTGG GGTCACCTAG CTTGCAGCAT AGCAGAGATG ATTCTTTGGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTCAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAATATT CCCCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCCTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAATATC GAACCAGCAC GAACTGCAGG AGTTAGTGGT 
CTTGCCAGGG CAGCCTCAAA AGACACCATA TCCAATAATA GAGAAAAAAT TAAAGGCTGG 
ATTAAGGAGC AGGCACATAA ATTTGTTGAG CGTTATTTTA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CCTTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCTTCAT TTGAAATCCA ACATAGTGGG TTTGTGAAAC AACTGTTGCT GTATTTGACA 
TCTAAGAATG AAAAGGATGC TGTCGGCAGA GAGATCCGGT TAAAACGTTT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCTGTTGGAA GAGTAGAACC AGTGGGCCAT 
GCACCTTTGC TGGCATTGGT TCACAAGATG AACAACTGTC TCAGCCAAAT GGAGCAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGG AACGGAGCTG GAGGAAGCTT TTCTCTCAAC 
AGAGGATCAC AAGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGTCA GTTACAAAGA 
CACCCAGACT GTGCAAATGT GAAGCAGTGG AAAGGCGGGC CTGTCAAGAT TGATCCCCTG 
GCTCTGGTGC AAGCTATTGA GAGATACCTT GTGGTTAGAG GATATGGAAG AGTAAGAGAA 
GATGATGAAG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAGTC TTTGGCTGCT 
CAATTCTTAA ATTCGGGAAA TGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTA 
CTACCATATA ACATGACCGT GTATCAGGCA GTGCGGCAAT TCAGTGTACA GGCTGAAGAT 
GAAAGAGAAT CTACTGATGA TGAGAGCAAT CCTCTGGGAA GAGCTGGAAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGG GAGGATGAAG AAAGTACTAA AGATTGTGTT 
GGTGGCAAAA GGGGGAGAGC CCAAACAGCT CCAACAAAAA CTTCCCCCAG AAATGCAAAG 
AAGCATGATG AGTTATGGCA TGATGGAGTG TGCCCGTCAG TAGCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATC ACCTTTGAAG ACCCATCCTT AGATGTAATA 
CTTCTTTTAA GAGTTTTACA TGCCATCAGT CGATATTGGT ATTACTTGTA TGATAATGCG 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAATTAAC AGCAAAAGCA 
AATAGGCAGC TTCAAGACCC TCTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTCACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGACAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGGGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA CAGCAGAGTT GCGCCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTACT GAAACAAGCT GAGTCTGTGA TGCAGGACCT TGGCAGCTCT 
CGGGCCATGT TAGAAATCCA ATATGAAAAT GAGGTTGGTA CAGGTCTGGG GCCAACACTG 
GAGTTTTATG CACTTGTTTC TCAGGAACTA CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA CATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCTA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCTCTTGG CCTGCCCTTT TATAAGTGGA TGCTGCGGCA GGAAACTTCA 
TTGACATCTC ATGATTTATT TGACATTGAT CCTGTTGTAG CCAGATCAGT ATATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTG GAGCAGGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTGGAAAC TCTTACGATG AATGGCTGTT CAGTGGAAGA TCTGGGATTG 
GATTTTACGC TGCCTGGGTT TCCCAACATT GAATTGAAGA AAGGAGGGAA GGATATTCCA 
GTTACTATCC ACAATTTGGA AGAGTACCTG AGATTGGTGA TATTCTGGGC ACTAAATGAA 
GGTGTTTGTC GACAGTTTGA CTCATTCAGA GATGGATTTG AATCGGTCTT CCCGCTCTGC 
CATCTTCAGT ACTTCTACCC AGAAGAGCTG GACCAGCTCC TGTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACATT GATGGAATGC TGCAGGCCTG ATCATGGCTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA CGATTGCCAG TTGGAGGATT TCGGAGTTTA 
AATCCACCTC TGACAATTGT GCGGAAGACA TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTACCATCAG TAATGACTTG TGTGAACTAT CTTAAATTGC CGGACTACTC GAGCATTGAC 
ATAATGCGGG ACAAACTCTT GATAGCAGCA AGAGAAGGCC AACAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496485.1
CDS	400..6522
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496422.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496422.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACACTTCA CTTCCTGCTA TGTGGTGCCT CCAATGGAAG TTGTCAAGAG 
CAGATTGACC TTGTTCCACG AAGTCCTCAA GAGCTGTATG AGCTGACATC TTTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATCTTCGCAG TTGATACCAT GTTGAAAAAG 
GGAAATGCAC AGAACACAGA TGGTGCAATA TGGCAGTGGC GGGATGACCG AGGCCTCTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAAGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAATCCC TTAGCCAATT CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTAATGAAAG AAGATCCGGA ACTGGCTAAG 
TCTTTCATTA AGACCTTGTT TGGTGTTCTC TATGAAGTGT ACAGCTCCTC AGCAGGCCCT 
GCGGTCAGAC ACAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC TGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC AATGCTATCC 
AGCCAAGACC TGAAGATAGT AGTTGGAGCT CTTCAGATGG CAGAAATCCT AATGCAAAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGG AGAGAAGGTG TGATGCATCA AGTCAAACAT 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCCCCCAAGG CATGTACAAA TGGGTCAGGA 
TCCCTGGGAT CTACAACACC AGCCAGCAGT GGGACTGCAA CGGCAGCAAC CAACGCCTCT 
GCTGATCTGG GGTCACCTAG CTTGCAGCAT AGCAGAGATG ATTCTTTGGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTCAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAATATT CCCCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCCTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAATATC GAACCAGCAC GAACTGCAGG AGTTAGTGGT 
CTTGCCAGGG CAGCCTCAAA AGACACCATA TCCAATAATA GAGAAAAAAT TAAAGGCTGG 
ATTAAGGAGC AGGCACATAA ATTTGTTGAG CGTTATTTTA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CCTTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCTTCAT TTGAAATCCA ACATAGTGGG TTTGTGAAAC AACTGTTGCT GTATTTGACA 
TCTAAGAATG AAAAGGATGC TGTCGGCAGA GAGATCCGGT TAAAACGTTT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCTGTTGGAA GAGTAGAACC AGTGGGCCAT 
GCACCTTTGC TGGCATTGGT TCACAAGATG AACAACTGTC TCAGCCAAAT GGAGCAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGG AACGGAGCTG GAGGAAGCTT TTCTCTCAAC 
AGAGGATCAC AAGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGTCA GTTACAAAGA 
CACCCAGACT GTGCAAATGT GAAGCAGTGG AAAGGCGGGC CTGTCAAGAT TGATCCCCTG 
GCTCTGGTGC AAGCTATTGA GAGATACCTT GTGGTTAGAG GATATGGAAG AGTAAGAGAA 
GATGATGAAG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAGTC TTTGGCTGCT 
CAATTCTTAA ATTCGGGAAA TGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTA 
CTACCATATA ACATGACCGT GTATCAGGCA GTGCGGCAAT TCAGTGTACA GGCTGAAGAT 
GAAAGAGAAT CTACTGATGA TGAGAGCAAT CCTCTGGGAA GAGCTGGAAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGG GAGGATGAAG AAAGTACTAA AGATTGTGTT 
GGTGGCAAAA GGGGGAGAGC CCAAACAGCT CCAACAAAAA CTTCCCCCAG AAATGCAAAG 
AAGCATGATG AGTTATGGCA TGATGGAGTG TGCCCGTCAG TAGCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATC ACCTTTGAAG ACCCATCCTT AGATGTAATA 
CTTCTTTTAA GAGTTTTACA TGCCATCAGT CGATATTGGT ATTACTTGTA TGATAATGCG 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAATTAAC AGCAAAAGCA 
AATAGGCAGC TTCAAGACCC TCTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTCACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGACAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGGGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA CAGCAGAGTT GCGCCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTACT GAAACAAGCT GAGTCTGTGA TGCAGGACCT TGGCAGCTCT 
CGGGCCATGT TAGAAATCCA ATATGAAAAT GAGGTTGGTA CAGGTCTGGG GCCAACACTG 
GAGTTTTATG CACTTGTTTC TCAGGAACTA CAGAGAGCTG ACTTGTGTCT CTGGAGGGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA CATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCTA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCTCTTGG CCTGCCCTTT TATAAGTGGA TGCTGCGGCA GGAAACTTCA 
TTGACATCTC ATGATTTATT TGACATTGAT CCTGTTGTAG CCAGATCAGT ATATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTG GAGCAGGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTGGAAAC TCTTACGATG AATGGCTGTT CAGTGGAAGA TCTGGGATTG 
GATTTTACGC TGCCTGGGTT TCCCAACATT GAATTGAAGA AAGGAGGGAA GGATATTCCA 
GTTACTATCC ACAATTTGGA AGAGTACCTG AGATTGGTGA TATTCTGGGC ACTAAATGAA 
GGTGTTTGTC GACAGTTTGA CTCATTCAGA GATGGATTTG AATCGGTCTT CCCGCTCTGC 
CATCTTCAGT ACTTCTACCC AGAAGAGCTG GACCAGCTCC TGTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACATT GATGGAATGC TGCAGGCCTG ATCATGGCTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC TGGTAGCCCA CGATTGCCAG TTGGAGGATT TCGGAGTTTA 
AATCCACCTC TGACAATTGT GCGGAAGACA TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTACCATCAG TAATGACTTG TGTGAACTAT CTTAAATTGC CGGACTACTC GAGCATTGAC 
ATAATGCGGG ACAAACTCTT GATAGCAGCA AGAGAAGGCC AACAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_006496427.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496490.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X28, mRNA.

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CloneID	OMu27720
Clone ID Related Accession (Same CDS sequence)	XM_006496427.3 , XM_006496427.2
Accession Version	XM_006496427.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAAGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ATCCCTTAGC CAATTCTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAT 
CCGGAACTGG CTAAGTCTTT CATTAAGACC TTGTTTGGTG TTCTCTATGA AGTGTACAGC 
TCCTCAGCAG GCCCTGCGGT CAGACACAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCTGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCAATGC TATCCAGCCA AGACCTGAAG ATAGTAGTTG GAGCTCTTCA GATGGCAGAA 
ATCCTAATGC AAAAGTTACC TGATATTTTT AGTGTTTACT TCAGGAGAGA AGGTGTGATG 
CATCAAGTCA AACATTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCCCC CAAGGCATGT 
ACAAATGGGT CAGGATCCCT GGGATCTACA ACACCAGCCA GCAGTGGGAC TGCAACGGCA 
GCAACCAACG CCTCTGCTGA TCTGGGGTCA CCTAGCTTGC AGCATAGCAG AGATGATTCT 
TTGGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTCA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA ATATTCCCCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCCT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ATATCGAACC AGCACGAACT 
GCAGGAGTTA GTGGTCTTGC CAGGGCAGCC TCAAAAGACA CCATATCCAA TAATAGAGAA 
AAAATTAAAG GCTGGATTAA GGAGCAGGCA CATAAATTTG TTGAGCGTTA TTTTAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCCTTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC TTCATTTGAA ATCCAACATA GTGGGTTTGT GAAACAACTG 
TTGCTGTATT TGACATCTAA GAATGAAAAG GATGCTGTCG GCAGAGAGAT CCGGTTAAAA 
CGTTTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCTGT TGGAAGAGTA 
GAACCAGTGG GCCATGCACC TTTGCTGGCA TTGGTTCACA AGATGAACAA CTGTCTCAGC 
CAAATGGAGC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGGAACGG AGCTGGAGGA 
AGCTTTTCTC TCAACAGAGG ATCACAAGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGTCAGTTAC AAAGACACCC AGACTGTGCA AATGTGAAGC AGTGGAAAGG CGGGCCTGTC 
AAGATTGATC CCCTGGCTCT GGTGCAAGCT ATTGAGAGAT ACCTTGTGGT TAGAGGTATT 
TTCCGATATG GAAGAGTAAG AGAAGATGAT GAAGATAGTG ATGATGATGG GTCAGATGAA 
GAAATAGATG AGTCTTTGGC TGCTCAATTC TTAAATTCGG GAAATGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTACTACCA TATAACATGA CCGTGTATCA GGCAGTGCGG 
CAATTCAGTG TACAGGCTGA AGATGAAAGA GAATCTACTG ATGATGAGAG CAATCCTCTG 
GGAAGAGCTG GAATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGGGAGGAT 
GAAGAAAGTA CTAAAGATTG TGTTGGTGGC AAAAGGGGGA GAGCCCAAAC AGCTCCAACA 
AAAACTTCCC CCAGAAATGC AAAGAAGCAT GATGAGTTAT GGCATGATGG AGTGTGCCCG 
TCAGTAGCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATCACCTTT 
GAAGACCCAT CCTTAGATGT AATACTTCTT TTAAGAGTTT TACATGCCAT CAGTCGATAT 
TGGTATTACT TGTATGATAA TGCGATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAAT TAACAGCAAA AGCAAATAGG CAGCTTCAAG ACCCTCTAGT AATCATGACA 
GGAAACATCC CAACATGGCT CACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGAC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGGGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGACAGCAG AGTTGCGCCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TACTGAAACA AGCTGAGTCT 
GTGATGCAGG ACCTTGGCAG CTCTCGGGCC ATGTTAGAAA TCCAATATGA AAATGAGGTT 
GGTACAGGTC TGGGGCCAAC ACTGGAGTTT TATGCACTTG TTTCTCAGGA ACTACAGAGA 
GCTGACTTGT GTCTCTGGAG GGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCACATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCTAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCTC TTGGCCTGCC CTTTTATAAG 
TGGATGCTGC GGCAGGAAAC TTCATTGACA TCTCATGATT TATTTGACAT TGATCCTGTT 
GTAGCCAGAT CAGTATATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTGGAGCAG 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTGG AAACTCTTAC GATGAATGGC 
TGTTCAGTGG AAGATCTGGG ATTGGATTTT ACGCTGCCTG GGTTTCCCAA CATTGAATTG 
AAGAAAGGAG GGAAGGATAT TCCAGTTACT ATCCACAATT TGGAAGAGTA CCTGAGATTG 
GTGATATTCT GGGCACTAAA TGAAGGTGTT TGTCGACAGT TTGACTCATT CAGAGATGGA 
TTTGAATCGG TCTTCCCGCT CTGCCATCTT CAGTACTTCT ACCCAGAAGA GCTGGACCAG 
CTCCTGTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CATTGATGGA ATGCTGCAGG 
CCTGATCATG GCTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACTGGTAG CCCACGATTG 
CCAGTTGGAG GATTTCGGAG TTTAAATCCA CCTCTGACAA TTGTGCGGAA GACATTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTACCA TCAGTAATGA CTTGTGTGAA CTATCTTAAA 
TTGCCGGACT ACTCGAGCAT TGACATAATG CGGGACAAAC TCTTGATAGC AGCAAGAGAA 
GGCCAACAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496490.1
CDS	167..6253
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006496427.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496427.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAAGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ATCCCTTAGC CAATTCTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAT 
CCGGAACTGG CTAAGTCTTT CATTAAGACC TTGTTTGGTG TTCTCTATGA AGTGTACAGC 
TCCTCAGCAG GCCCTGCGGT CAGACACAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCTGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCAATGC TATCCAGCCA AGACCTGAAG ATAGTAGTTG GAGCTCTTCA GATGGCAGAA 
ATCCTAATGC AAAAGTTACC TGATATTTTT AGTGTTTACT TCAGGAGAGA AGGTGTGATG 
CATCAAGTCA AACATTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCCCC CAAGGCATGT 
ACAAATGGGT CAGGATCCCT GGGATCTACA ACACCAGCCA GCAGTGGGAC TGCAACGGCA 
GCAACCAACG CCTCTGCTGA TCTGGGGTCA CCTAGCTTGC AGCATAGCAG AGATGATTCT 
TTGGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTCA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA ATATTCCCCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCCT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ATATCGAACC AGCACGAACT 
GCAGGAGTTA GTGGTCTTGC CAGGGCAGCC TCAAAAGACA CCATATCCAA TAATAGAGAA 
AAAATTAAAG GCTGGATTAA GGAGCAGGCA CATAAATTTG TTGAGCGTTA TTTTAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCCTTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC TTCATTTGAA ATCCAACATA GTGGGTTTGT GAAACAACTG 
TTGCTGTATT TGACATCTAA GAATGAAAAG GATGCTGTCG GCAGAGAGAT CCGGTTAAAA 
CGTTTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCTGT TGGAAGAGTA 
GAACCAGTGG GCCATGCACC TTTGCTGGCA TTGGTTCACA AGATGAACAA CTGTCTCAGC 
CAAATGGAGC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGGAACGG AGCTGGAGGA 
AGCTTTTCTC TCAACAGAGG ATCACAAGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGTCAGTTAC AAAGACACCC AGACTGTGCA AATGTGAAGC AGTGGAAAGG CGGGCCTGTC 
AAGATTGATC CCCTGGCTCT GGTGCAAGCT ATTGAGAGAT ACCTTGTGGT TAGAGGTATT 
TTCCGATATG GAAGAGTAAG AGAAGATGAT GAAGATAGTG ATGATGATGG GTCAGATGAA 
GAAATAGATG AGTCTTTGGC TGCTCAATTC TTAAATTCGG GAAATGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTACTACCA TATAACATGA CCGTGTATCA GGCAGTGCGG 
CAATTCAGTG TACAGGCTGA AGATGAAAGA GAATCTACTG ATGATGAGAG CAATCCTCTG 
GGAAGAGCTG GAATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGGGAGGAT 
GAAGAAAGTA CTAAAGATTG TGTTGGTGGC AAAAGGGGGA GAGCCCAAAC AGCTCCAACA 
AAAACTTCCC CCAGAAATGC AAAGAAGCAT GATGAGTTAT GGCATGATGG AGTGTGCCCG 
TCAGTAGCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATCACCTTT 
GAAGACCCAT CCTTAGATGT AATACTTCTT TTAAGAGTTT TACATGCCAT CAGTCGATAT 
TGGTATTACT TGTATGATAA TGCGATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAAT TAACAGCAAA AGCAAATAGG CAGCTTCAAG ACCCTCTAGT AATCATGACA 
GGAAACATCC CAACATGGCT CACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGAC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGGGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGACAGCAG AGTTGCGCCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TACTGAAACA AGCTGAGTCT 
GTGATGCAGG ACCTTGGCAG CTCTCGGGCC ATGTTAGAAA TCCAATATGA AAATGAGGTT 
GGTACAGGTC TGGGGCCAAC ACTGGAGTTT TATGCACTTG TTTCTCAGGA ACTACAGAGA 
GCTGACTTGT GTCTCTGGAG GGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCACATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCTAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCTC TTGGCCTGCC CTTTTATAAG 
TGGATGCTGC GGCAGGAAAC TTCATTGACA TCTCATGATT TATTTGACAT TGATCCTGTT 
GTAGCCAGAT CAGTATATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTGGAGCAG 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTGG AAACTCTTAC GATGAATGGC 
TGTTCAGTGG AAGATCTGGG ATTGGATTTT ACGCTGCCTG GGTTTCCCAA CATTGAATTG 
AAGAAAGGAG GGAAGGATAT TCCAGTTACT ATCCACAATT TGGAAGAGTA CCTGAGATTG 
GTGATATTCT GGGCACTAAA TGAAGGTGTT TGTCGACAGT TTGACTCATT CAGAGATGGA 
TTTGAATCGG TCTTCCCGCT CTGCCATCTT CAGTACTTCT ACCCAGAAGA GCTGGACCAG 
CTCCTGTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CATTGATGGA ATGCTGCAGG 
CCTGATCATG GCTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACTGGTAG CCCACGATTG 
CCAGTTGGAG GATTTCGGAG TTTAAATCCA CCTCTGACAA TTGTGCGGAA GACATTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTACCA TCAGTAATGA CTTGTGTGAA CTATCTTAAA 
TTGCCGGACT ACTCGAGCAT TGACATAATG CGGGACAAAC TCTTGATAGC AGCAAGAGAA 
GGCCAACAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238655.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236957.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X2, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238655.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236957.1
CDS	125..6349
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238655.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238655.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_017316494.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171983.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X8, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_017316494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171983.1
CDS	492..6716
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017316494.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316494.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238654.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236956.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X1, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238654.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236956.1
CDS	400..6624
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238654.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238654.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238658.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236960.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X5, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238658.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236960.1
CDS	115..6339
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238658.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238658.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238659.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236961.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X6, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238659.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236961.1
CDS	115..6339
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238659.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238659.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238657.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236959.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X4, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238657.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236959.1
CDS	260..6484
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238657.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238657.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238660.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236962.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X7, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238660.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236962.1
CDS	72..6296
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238660.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238660.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238656.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236958.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X3, mRNA.

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CloneID	OMu67884
Clone ID Related Accession (Same CDS sequence)	XM_011238655.2 , XM_017316494.1 , XM_011238654.2 , XM_011238658.2 , XM_011238654.3 , XM_011238655.3 , XM_011238657.3 , XM_011238659.2 , XM_011238660.3 , XM_011238657.2 , XM_011238660.2 , XM_011238658.3 , XM_011238656.2 , XM_011238656.3 , XM_030248874.1
Accession Version	XM_011238656.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236958.1
CDS	178..6402
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238656.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238656.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGTATTTT CCGATATGGA AGAGTAAGAG AAGATGATGA AGATAGTGAT 
GATGATGGGT CAGATGAAGA AATAGATGAG TCTTTGGCTG CTCAATTCTT AAATTCGGGA 
AATGTAAGGC ACAGGCTACA GTTTTATATT GGAGAACATT TACTACCATA TAACATGACC 
GTGTATCAGG CAGTGCGGCA ATTCAGTGTA CAGGCTGAAG ATGAAAGAGA ATCTACTGAT 
GATGAGAGCA ATCCTCTGGG AAGAGCTGGA ATTTGGACAA AGACTCATAC AATATGGTAC 
AAACCTGTGA GGGAGGATGA AGAAAGTACT AAAGATTGTG TTGGTGGCAA AAGGGGGAGA 
GCCCAAACAG CTCCAACAAA AACTTCCCCC AGAAATGCAA AGAAGCATGA TGAGTTATGG 
CATGATGGAG TGTGCCCGTC AGTAGCAAAT CCTTTAGAAG TTTACCTCAT TCCCACACCA 
CCTGAAAATA TCACCTTTGA AGACCCATCC TTAGATGTAA TACTTCTTTT AAGAGTTTTA 
CATGCCATCA GTCGATATTG GTATTACTTG TATGATAATG CGATGTGCAA GGAGATTATT 
CCAACTAGTG AATTTATTAA CAGTAAATTA ACAGCAAAAG CAAATAGGCA GCTTCAAGAC 
CCTCTAGTAA TCATGACAGG AAACATCCCA ACATGGCTCA CTGAGCTAGG AAAAACCTGC 
CCATTTTTCT TTCCTTTTGA TACCCGACAA ATGCTTTTTT ATGTAACTGC ATTTGATCGG 
GACCGGGCAA TGCAAAGATT ACTTGATACC AACCCAGAAA TCAACCAGTC TGATTCTCAA 
GACAGCAGAG TTGCGCCTAG ATTGGATAGA AAAAAACGTA CTGTGAACAG AGAGGAGCTA 
CTGAAACAAG CTGAGTCTGT GATGCAGGAC CTTGGCAGCT CTCGGGCCAT GTTAGAAATC 
CAATATGAAA ATGAGGTTGG TACAGGTCTG GGGCCAACAC TGGAGTTTTA TGCACTTGTT 
TCTCAGGAAC TACAGAGAGC TGACTTGTGT CTCTGGAGGG GTGAAGAAGT AACTCTTAGC 
AATCCAAAAG GAAGCCAAGA AGGGACCAAG TATATTCAAA ACCTCCAGGG CCTGTTTGCG 
CTTCCCTTTG GTAGGACAGC TAAGCCAGCT CACATCGCAA AGGTTAAGAT GAAGTTTCGC 
TTCTTAGGAA AATTAATGGC TAAGGCTATC ATGGATTTCA GATTGGTGGA CCTTCCTCTT 
GGCCTGCCCT TTTATAAGTG GATGCTGCGG CAGGAAACTT CATTGACATC TCATGATTTA 
TTTGACATTG ATCCTGTTGT AGCCAGATCA GTATATCACC TAGAAGACAT TGTCAGACAG 
AAGAAAAGAC TGGAGCAGGA TAAATCCCAG ACCAAAGAGA GTCTACAATA TGCATTGGAA 
ACTCTTACGA TGAATGGCTG TTCAGTGGAA GATCTGGGAT TGGATTTTAC GCTGCCTGGG 
TTTCCCAACA TTGAATTGAA GAAAGGAGGG AAGGATATTC CAGTTACTAT CCACAATTTG 
GAAGAGTACC TGAGATTGGT GATATTCTGG GCACTAAATG AAGGTGTTTG TCGACAGTTT 
GACTCATTCA GAGATGGATT TGAATCGGTC TTCCCGCTCT GCCATCTTCA GTACTTCTAC 
CCAGAAGAGC TGGACCAGCT CCTGTGTGGC AGTAAAGCAG ACACTTGGGA TGCAAAGACA 
TTGATGGAAT GCTGCAGGCC TGATCATGGC TATACTCATG ACAGTCGGGC TGTGAAGTTC 
TTGTTTGAGA TTCTCAGTAG TTTTGATAAT GAGCAGCAGA GGTTGTTTCT CCAGTTTGTG 
ACTGGTAGCC CACGATTGCC AGTTGGAGGA TTTCGGAGTT TAAATCCACC TCTGACAATT 
GTGCGGAAGA CATTTGAATC AACAGAAAAC CCAGATGACT TCTTACCATC AGTAATGACT 
TGTGTGAACT ATCTTAAATT GCCGGACTAC TCGAGCATTG ACATAATGCG GGACAAACTC 
TTGATAGCAG CAAGAGAAGG CCAACAGTCG TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238661.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236963.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X9, mRNA.

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CloneID	OMu67885
Clone ID Related Accession (Same CDS sequence)	XM_011238661.2 , XM_011238661.3
Accession Version	XM_011238661.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAAGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAATCCC 
TTAGCCAATT CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTAATGAAAG AAGATCCGGA ACTGGCTAAG TCTTTCATTA AGACCTTGTT TGGTGTTCTC 
TATGAAGTGT ACAGCTCCTC AGCAGGCCCT GCGGTCAGAC ACAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTTGC TGATGCTGAA CTTCTGAAGG ATGTTCTGAA AAATCATGCT 
GTTTCAAGTC ACATTGCTTC AATGCTATCC AGCCAAGACC TGAAGATAGT AGTTGGAGCT 
CTTCAGATGG CAGAAATCCT AATGCAAAAG TTACCTGATA TTTTTAGTGT TTACTTCAGG 
AGAGAAGGTG TGATGCATCA AGTCAAACAT TTAGCAGAAT CAGAGTCTTT GTTGACAAGT 
CCCCCCAAGG CATGTACAAA TGGGTCAGGA TCCCTGGGAT CTACAACACC AGCCAGCAGT 
GGGACTGCAA CGGCAGCAAC CAACGCCTCT GCTGATCTGG GGTCACCTAG CTTGCAGCAT 
AGCAGAGATG ATTCTTTGGA TCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTCAAGAGA 
AAACGACTGC CAAAACGAGG GCCAAGAAGG CCAAAATATT CCCCTCCAAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACC ACTACTCAGT CACCTAAATC TTCCTTCCTG 
GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG TTAAGCGCAC AGTCCAACAG CAACAATATC 
GAACCAGCAC GAACTGCAGG AGTTAGTGGT CTTGCCAGGG CAGCCTCAAA AGACACCATA 
TCCAATAATA GAGAAAAAAT TAAAGGCTGG ATTAAGGAGC AGGCACATAA ATTTGTTGAG 
CGTTATTTTA GTTCTGAGAA TATGGATGGA AGCAACCCTG CCTTGAATGT CCTTCAGAGA 
CTTTGTGCTG CAACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCAGAT GTTTCTTCAT TTGAAATCCA ACATAGTGGG 
TTTGTGAAAC AACTGTTGCT GTATTTGACA TCTAAGAATG AAAAGGATGC TGTCGGCAGA 
GAGATCCGGT TAAAACGTTT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCTGTTGGAA GAGTAGAACC AGTGGGCCAT GCACCTTTGC TGGCATTGGT TCACAAGATG 
AACAACTGTC TCAGCCAAAT GGAGCAATTT CCAGTCAAAG TACATGATTT CCCTAGTGGG 
AACGGAGCTG GAGGAAGCTT TTCTCTCAAC AGAGGATCAC AAGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGTCA GTTACAAAGA CACCCAGACT GTGCAAATGT GAAGCAGTGG 
AAAGGCGGGC CTGTCAAGAT TGATCCCCTG GCTCTGGTGC AAGCTATTGA GAGATACCTT 
GTGGTTAGAG GTATTTTCCG ATATGGAAGA GTAAGAGAAG ATGATGAAGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAGTCT TTGGCTGCTC AATTCTTAAA TTCGGGAAAT 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTAC TACCATATAA CATGACCGTG 
TATCAGGCAG TGCGGCAATT CAGTGTACAG GCTGAAGATG AAAGAGAATC TACTGATGAT 
GAGAGCAATC CTCTGGGAAG AGCTGGAATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGGG AGGATGAAGA AAGTACTAAA GATTGTGTTG GTGGCAAAAG GGGGAGAGCC 
CAAACAGCTC CAACAAAAAC TTCCCCCAGA AATGCAAAGA AGCATGATGA GTTATGGCAT 
GATGGAGTGT GCCCGTCAGT AGCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATCA CCTTTGAAGA CCCATCCTTA GATGTAATAC TTCTTTTAAG AGTTTTACAT 
GCCATCAGTC GATATTGGTA TTACTTGTAT GATAATGCGA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAATTAACA GCAAAAGCAA ATAGGCAGCT TCAAGACCCT 
CTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTCACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGACAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGGGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAC 
AGCAGAGTTG CGCCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTACTG 
AAACAAGCTG AGTCTGTGAT GCAGGACCTT GGCAGCTCTC GGGCCATGTT AGAAATCCAA 
TATGAAAATG AGGTTGGTAC AGGTCTGGGG CCAACACTGG AGTTTTATGC ACTTGTTTCT 
CAGGAACTAC AGAGAGCTGA CTTGTGTCTC TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAC ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCTAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCTCTTGGC 
CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG GAAACTTCAT TGACATCTCA TGATTTATTT 
GACATTGATC CTGTTGTAGC CAGATCAGTA TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTGG AGCAGGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTGGAAACT 
CTTACGATGA ATGGCTGTTC AGTGGAAGAT CTGGGATTGG ATTTTACGCT GCCTGGGTTT 
CCCAACATTG AATTGAAGAA AGGAGGGAAG GATATTCCAG TTACTATCCA CAATTTGGAA 
GAGTACCTGA GATTGGTGAT ATTCTGGGCA CTAAATGAAG GTGTTTGTCG ACAGTTTGAC 
TCATTCAGAG ATGGATTTGA ATCGGTCTTC CCGCTCTGCC ATCTTCAGTA CTTCTACCCA 
GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACATTG 
ATGGAATGCT GCAGGCCTGA TCATGGCTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACT 
GGTAGCCCAC GATTGCCAGT TGGAGGATTT CGGAGTTTAA ATCCACCTCT GACAATTGTG 
CGGAAGACAT TTGAATCAAC AGAAAACCCA GATGACTTCT TACCATCAGT AATGACTTGT 
GTGAACTATC TTAAATTGCC GGACTACTCG AGCATTGACA TAATGCGGGA CAAACTCTTG 
ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236963.1
CDS	400..6621
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238661.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238661.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAAGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAATCCC 
TTAGCCAATT CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTAATGAAAG AAGATCCGGA ACTGGCTAAG TCTTTCATTA AGACCTTGTT TGGTGTTCTC 
TATGAAGTGT ACAGCTCCTC AGCAGGCCCT GCGGTCAGAC ACAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTTGC TGATGCTGAA CTTCTGAAGG ATGTTCTGAA AAATCATGCT 
GTTTCAAGTC ACATTGCTTC AATGCTATCC AGCCAAGACC TGAAGATAGT AGTTGGAGCT 
CTTCAGATGG CAGAAATCCT AATGCAAAAG TTACCTGATA TTTTTAGTGT TTACTTCAGG 
AGAGAAGGTG TGATGCATCA AGTCAAACAT TTAGCAGAAT CAGAGTCTTT GTTGACAAGT 
CCCCCCAAGG CATGTACAAA TGGGTCAGGA TCCCTGGGAT CTACAACACC AGCCAGCAGT 
GGGACTGCAA CGGCAGCAAC CAACGCCTCT GCTGATCTGG GGTCACCTAG CTTGCAGCAT 
AGCAGAGATG ATTCTTTGGA TCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTCAAGAGA 
AAACGACTGC CAAAACGAGG GCCAAGAAGG CCAAAATATT CCCCTCCAAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACC ACTACTCAGT CACCTAAATC TTCCTTCCTG 
GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG TTAAGCGCAC AGTCCAACAG CAACAATATC 
GAACCAGCAC GAACTGCAGG AGTTAGTGGT CTTGCCAGGG CAGCCTCAAA AGACACCATA 
TCCAATAATA GAGAAAAAAT TAAAGGCTGG ATTAAGGAGC AGGCACATAA ATTTGTTGAG 
CGTTATTTTA GTTCTGAGAA TATGGATGGA AGCAACCCTG CCTTGAATGT CCTTCAGAGA 
CTTTGTGCTG CAACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCAGAT GTTTCTTCAT TTGAAATCCA ACATAGTGGG 
TTTGTGAAAC AACTGTTGCT GTATTTGACA TCTAAGAATG AAAAGGATGC TGTCGGCAGA 
GAGATCCGGT TAAAACGTTT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCTGTTGGAA GAGTAGAACC AGTGGGCCAT GCACCTTTGC TGGCATTGGT TCACAAGATG 
AACAACTGTC TCAGCCAAAT GGAGCAATTT CCAGTCAAAG TACATGATTT CCCTAGTGGG 
AACGGAGCTG GAGGAAGCTT TTCTCTCAAC AGAGGATCAC AAGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGTCA GTTACAAAGA CACCCAGACT GTGCAAATGT GAAGCAGTGG 
AAAGGCGGGC CTGTCAAGAT TGATCCCCTG GCTCTGGTGC AAGCTATTGA GAGATACCTT 
GTGGTTAGAG GTATTTTCCG ATATGGAAGA GTAAGAGAAG ATGATGAAGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAGTCT TTGGCTGCTC AATTCTTAAA TTCGGGAAAT 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTAC TACCATATAA CATGACCGTG 
TATCAGGCAG TGCGGCAATT CAGTGTACAG GCTGAAGATG AAAGAGAATC TACTGATGAT 
GAGAGCAATC CTCTGGGAAG AGCTGGAATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGGG AGGATGAAGA AAGTACTAAA GATTGTGTTG GTGGCAAAAG GGGGAGAGCC 
CAAACAGCTC CAACAAAAAC TTCCCCCAGA AATGCAAAGA AGCATGATGA GTTATGGCAT 
GATGGAGTGT GCCCGTCAGT AGCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATCA CCTTTGAAGA CCCATCCTTA GATGTAATAC TTCTTTTAAG AGTTTTACAT 
GCCATCAGTC GATATTGGTA TTACTTGTAT GATAATGCGA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAATTAACA GCAAAAGCAA ATAGGCAGCT TCAAGACCCT 
CTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTCACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGACAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGGGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAC 
AGCAGAGTTG CGCCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTACTG 
AAACAAGCTG AGTCTGTGAT GCAGGACCTT GGCAGCTCTC GGGCCATGTT AGAAATCCAA 
TATGAAAATG AGGTTGGTAC AGGTCTGGGG CCAACACTGG AGTTTTATGC ACTTGTTTCT 
CAGGAACTAC AGAGAGCTGA CTTGTGTCTC TGGAGGGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAC ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCTAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCTCTTGGC 
CTGCCCTTTT ATAAGTGGAT GCTGCGGCAG GAAACTTCAT TGACATCTCA TGATTTATTT 
GACATTGATC CTGTTGTAGC CAGATCAGTA TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTGG AGCAGGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTGGAAACT 
CTTACGATGA ATGGCTGTTC AGTGGAAGAT CTGGGATTGG ATTTTACGCT GCCTGGGTTT 
CCCAACATTG AATTGAAGAA AGGAGGGAAG GATATTCCAG TTACTATCCA CAATTTGGAA 
GAGTACCTGA GATTGGTGAT ATTCTGGGCA CTAAATGAAG GTGTTTGTCG ACAGTTTGAC 
TCATTCAGAG ATGGATTTGA ATCGGTCTTC CCGCTCTGCC ATCTTCAGTA CTTCTACCCA 
GAAGAGCTGG ACCAGCTCCT GTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACATTG 
ATGGAATGCT GCAGGCCTGA TCATGGCTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACT 
GGTAGCCCAC GATTGCCAGT TGGAGGATTT CGGAGTTTAA ATCCACCTCT GACAATTGTG 
CGGAAGACAT TTGAATCAAC AGAAAACCCA GATGACTTCT TACCATCAGT AATGACTTGT 
GTGAACTATC TTAAATTGCC GGACTACTCG AGCATTGACA TAATGCGGGA CAAACTCTTG 
ATAGCAGCAA GAGAAGGCCA ACAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238662.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236964.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X10, mRNA.

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CloneID	OMu67886
Clone ID Related Accession (Same CDS sequence)	XM_011238662.2 , XM_011238662.3
Accession Version	XM_011238662.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGATATGG AAGAGTAAGA GAAGATGATG AAGATAGTGA TGATGATGGG 
TCAGATGAAG AAATAGATGA GTCTTTGGCT GCTCAATTCT TAAATTCGGG AAATGTAAGG 
CACAGGCTAC AGTTTTATAT TGGAGAACAT TTACTACCAT ATAACATGAC CGTGTATCAG 
GCAGTGCGGC AATTCAGTGT ACAGGCTGAA GATGAAAGAG AATCTACTGA TGATGAGAGC 
AATCCTCTGG GAAGAGCTGG AATTTGGACA AAGACTCATA CAATATGGTA CAAACCTGTG 
AGGGAGGATG AAGAAAGTAC TAAAGATTGT GTTGGTGGCA AAAGGGGGAG AGCCCAAACA 
GCTCCAACAA AAACTTCCCC CAGAAATGCA AAGAAGCATG ATGAGTTATG GCATGATGGA 
GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATCACCTTTG AAGACCCATC CTTAGATGTA ATACTTCTTT TAAGAGTTTT ACATGCCATC 
AGTCGATATT GGTATTACTT GTATGATAAT GCGATGTGCA AGGAGATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAATT AACAGCAAAA GCAAATAGGC AGCTTCAAGA CCCTCTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTC ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGACA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGGGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGACAGCAGA 
GTTGCGCCTA GATTGGATAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT ACTGAAACAA 
GCTGAGTCTG TGATGCAGGA CCTTGGCAGC TCTCGGGCCA TGTTAGAAAT CCAATATGAA 
AATGAGGTTG GTACAGGTCT GGGGCCAACA CTGGAGTTTT ATGCACTTGT TTCTCAGGAA 
CTACAGAGAG CTGACTTGTG TCTCTGGAGG GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCACATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CTAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCTCT TGGCCTGCCC 
TTTTATAAGT GGATGCTGCG GCAGGAAACT TCATTGACAT CTCATGATTT ATTTGACATT 
GATCCTGTTG TAGCCAGATC AGTATATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTGGAGCAGG ATAAATCCCA GACCAAAGAG AGTCTACAAT ATGCATTGGA AACTCTTACG 
ATGAATGGCT GTTCAGTGGA AGATCTGGGA TTGGATTTTA CGCTGCCTGG GTTTCCCAAC 
ATTGAATTGA AGAAAGGAGG GAAGGATATT CCAGTTACTA TCCACAATTT GGAAGAGTAC 
CTGAGATTGG TGATATTCTG GGCACTAAAT GAAGGTGTTT GTCGACAGTT TGACTCATTC 
AGAGATGGAT TTGAATCGGT CTTCCCGCTC TGCCATCTTC AGTACTTCTA CCCAGAAGAG 
CTGGACCAGC TCCTGTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ATTGATGGAA 
TGCTGCAGGC CTGATCATGG CTATACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTGTTTC TCCAGTTTGT GACTGGTAGC 
CCACGATTGC CAGTTGGAGG ATTTCGGAGT TTAAATCCAC CTCTGACAAT TGTGCGGAAG 
ACATTTGAAT CAACAGAAAA CCCAGATGAC TTCTTACCAT CAGTAATGAC TTGTGTGAAC 
TATCTTAAAT TGCCGGACTA CTCGAGCATT GACATAATGC GGGACAAACT CTTGATAGCA 
GCAAGAGAAG GCCAACAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236964.1
CDS	400..6615
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238662.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238662.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG CTTTTCTCTC AACAGAGGAT CACAAGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG TCAGTTACAA AGACACCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAAGGCG GGCCTGTCAA GATTGATCCC CTGGCTCTGG TGCAAGCTAT TGAGAGATAC 
CTTGTGGTTA GAGGATATGG AAGAGTAAGA GAAGATGATG AAGATAGTGA TGATGATGGG 
TCAGATGAAG AAATAGATGA GTCTTTGGCT GCTCAATTCT TAAATTCGGG AAATGTAAGG 
CACAGGCTAC AGTTTTATAT TGGAGAACAT TTACTACCAT ATAACATGAC CGTGTATCAG 
GCAGTGCGGC AATTCAGTGT ACAGGCTGAA GATGAAAGAG AATCTACTGA TGATGAGAGC 
AATCCTCTGG GAAGAGCTGG AATTTGGACA AAGACTCATA CAATATGGTA CAAACCTGTG 
AGGGAGGATG AAGAAAGTAC TAAAGATTGT GTTGGTGGCA AAAGGGGGAG AGCCCAAACA 
GCTCCAACAA AAACTTCCCC CAGAAATGCA AAGAAGCATG ATGAGTTATG GCATGATGGA 
GTGTGCCCGT CAGTAGCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATCACCTTTG AAGACCCATC CTTAGATGTA ATACTTCTTT TAAGAGTTTT ACATGCCATC 
AGTCGATATT GGTATTACTT GTATGATAAT GCGATGTGCA AGGAGATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAATT AACAGCAAAA GCAAATAGGC AGCTTCAAGA CCCTCTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTC ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGACA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGGGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGACAGCAGA 
GTTGCGCCTA GATTGGATAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT ACTGAAACAA 
GCTGAGTCTG TGATGCAGGA CCTTGGCAGC TCTCGGGCCA TGTTAGAAAT CCAATATGAA 
AATGAGGTTG GTACAGGTCT GGGGCCAACA CTGGAGTTTT ATGCACTTGT TTCTCAGGAA 
CTACAGAGAG CTGACTTGTG TCTCTGGAGG GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCACATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CTAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCTCT TGGCCTGCCC 
TTTTATAAGT GGATGCTGCG GCAGGAAACT TCATTGACAT CTCATGATTT ATTTGACATT 
GATCCTGTTG TAGCCAGATC AGTATATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTGGAGCAGG ATAAATCCCA GACCAAAGAG AGTCTACAAT ATGCATTGGA AACTCTTACG 
ATGAATGGCT GTTCAGTGGA AGATCTGGGA TTGGATTTTA CGCTGCCTGG GTTTCCCAAC 
ATTGAATTGA AGAAAGGAGG GAAGGATATT CCAGTTACTA TCCACAATTT GGAAGAGTAC 
CTGAGATTGG TGATATTCTG GGCACTAAAT GAAGGTGTTT GTCGACAGTT TGACTCATTC 
AGAGATGGAT TTGAATCGGT CTTCCCGCTC TGCCATCTTC AGTACTTCTA CCCAGAAGAG 
CTGGACCAGC TCCTGTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ATTGATGGAA 
TGCTGCAGGC CTGATCATGG CTATACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTGTTTC TCCAGTTTGT GACTGGTAGC 
CCACGATTGC CAGTTGGAGG ATTTCGGAGT TTAAATCCAC CTCTGACAAT TGTGCGGAAG 
ACATTTGAAT CAACAGAAAA CCCAGATGAC TTCTTACCAT CAGTAATGAC TTGTGTGAAC 
TATCTTAAAT TGCCGGACTA CTCGAGCATT GACATAATGC GGGACAAACT CTTGATAGCA 
GCAAGAGAAG GCCAACAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238663.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236965.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X13, mRNA.

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CloneID	OMu67887
Clone ID Related Accession (Same CDS sequence)	XM_011238663.3 , XM_011238663.2
Accession Version	XM_011238663.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236965.1
CDS	400..6609
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238663.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238663.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238664.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236966.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X15, mRNA.

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CloneID	OMu67888
Clone ID Related Accession (Same CDS sequence)	XM_011238664.3 , XM_011238664.2
Accession Version	XM_011238664.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236966.1
CDS	400..6600
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238664.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238664.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA AGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAT 
CCCTTAGCCA ATTCTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGATCC GGAACTGGCT AAGTCTTTCA TTAAGACCTT GTTTGGTGTT 
CTCTATGAAG TGTACAGCTC CTCAGCAGGC CCTGCGGTCA GACACAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCTGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCAATGCTA TCCAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCTCTTCAGA TGGCAGAAAT CCTAATGCAA AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGGAGAGAAG GTGTGATGCA TCAAGTCAAA CATTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCCCCCA AGGCATGTAC AAATGGGTCA GGATCCCTGG GATCTACAAC ACCAGCCAGC 
AGTGGGACTG CAACGGCAGC AACCAACGCC TCTGCTGATC TGGGGTCACC TAGCTTGCAG 
CATAGCAGAG ATGATTCTTT GGATCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTCAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAAT ATTCCCCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCCTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGCG CACAGTCCAA CAGCAACAAT 
ATCGAACCAG CACGAACTGC AGGAGTTAGT GGTCTTGCCA GGGCAGCCTC AAAAGACACC 
ATATCCAATA ATAGAGAAAA AATTAAAGGC TGGATTAAGG AGCAGGCACA TAAATTTGTT 
GAGCGTTATT TTAGTTCTGA GAATATGGAT GGAAGCAACC CTGCCTTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCTT CATTTGAAAT CCAACATAGT 
GGGTTTGTGA AACAACTGTT GCTGTATTTG ACATCTAAGA ATGAAAAGGA TGCTGTCGGC 
AGAGAGATCC GGTTAAAACG TTTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCTGTTG GAAGAGTAGA ACCAGTGGGC CATGCACCTT TGCTGGCATT GGTTCACAAG 
ATGAACAACT GTCTCAGCCA AATGGAGCAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGGAACGGAG CTGGAGGAAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238665.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236967.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X16, mRNA.

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CloneID	OMu67889
Clone ID Related Accession (Same CDS sequence)	XM_011238665.2 , XM_011238665.3
Accession Version	XM_011238665.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238665.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTACA AATCAATGAA 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAATC CCTTAGCCAA TTCTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTAATGAA AGAAGATCCG 
GAACTGGCTA AGTCTTTCAT TAAGACCTTG TTTGGTGTTC TCTATGAAGT GTACAGCTCC 
TCAGCAGGCC CTGCGGTCAG ACACAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTT 
GCTGATGCTG AACTTCTGAA GGATGTTCTG AAAAATCATG CTGTTTCAAG TCACATTGCT 
TCAATGCTAT CCAGCCAAGA CCTGAAGATA GTAGTTGGAG CTCTTCAGAT GGCAGAAATC 
CTAATGCAAA AGTTACCTGA TATTTTTAGT GTTTACTTCA GGAGAGAAGG TGTGATGCAT 
CAAGTCAAAC ATTTAGCAGA ATCAGAGTCT TTGTTGACAA GTCCCCCCAA GGCATGTACA 
AATGGGTCAG GATCCCTGGG ATCTACAACA CCAGCCAGCA GTGGGACTGC AACGGCAGCA 
ACCAACGCCT CTGCTGATCT GGGGTCACCT AGCTTGCAGC ATAGCAGAGA TGATTCTTTG 
GATCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTCAAGA GAAAACGACT GCCAAAACGA 
GGGCCAAGAA GGCCAAAATA TTCCCCTCCA AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CCACTACTCA GTCACCTAAA TCTTCCTTCC TGGCAAGCTT GAATCCAAAA 
ACATGGGGAA GGTTAAGCGC ACAGTCCAAC AGCAACAATA TCGAACCAGC ACGAACTGCA 
GGAGTTAGTG GTCTTGCCAG GGCAGCCTCA AAAGACACCA TATCCAATAA TAGAGAAAAA 
ATTAAAGGCT GGATTAAGGA GCAGGCACAT AAATTTGTTG AGCGTTATTT TAGTTCTGAG 
AATATGGATG GAAGCAACCC TGCCTTGAAT GTCCTTCAGA GACTTTGTGC TGCAACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCAG ATGTTTCTTC ATTTGAAATC CAACATAGTG GGTTTGTGAA ACAACTGTTG 
CTGTATTTGA CATCTAAGAA TGAAAAGGAT GCTGTCGGCA GAGAGATCCG GTTAAAACGT 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCTGTTGG AAGAGTAGAA 
CCAGTGGGCC ATGCACCTTT GCTGGCATTG GTTCACAAGA TGAACAACTG TCTCAGCCAA 
ATGGAGCAAT TTCCAGTCAA AGTACATGAT TTCCCTAGTG GGAACGGAGC TGGAGGAAGC 
TTTTCTCTCA ACAGAGGATC ACAAGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGT 
CAGTTACAAA GACACCCAGA CTGTGCAAAT GTGAAGCAGT GGAAAGGCGG GCCTGTCAAG 
ATTGATCCCC TGGCTCTGGT GCAAGCTATT GAGAGATACC TTGTGGTTAG AGGTATTTTC 
CGATATGGAA GAGTAAGAGA AGATGATGAA GATAGTGATG ATGATGGGTC AGATGAAGAA 
ATAGATGAGT CTTTGGCTGC TCAATTCTTA AATTCGGGAA ATGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT ACTACCATAT AACATGACCG TGTATCAGGC AGTGCGGCAA 
TTCAGTGTAC AGGCTGAAGA TGAAAGAGAA TCTACTGATG ATGAGAGCAA TCCTCTGGGA 
AGAGCTGGAA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG GGAGGATGAA 
GAAAGTACTA AAGATTGTGT TGGTGGCAAA AGGGGGAGAG CCCAAACAGC TCCAACAAAA 
ACTTCCCCCA GAAATGCAAA GAAGCATGAT GAGTTATGGC ATGATGGAGT GTGCCCGTCA 
GTAGCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT CACCTTTGAA 
GACCCATCCT TAGATGTAAT ACTTCTTTTA AGAGTTTTAC ATGCCATCAG TCGATATTGG 
TATTACTTGT ATGATAATGC GATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAATTAA CAGCAAAAGC AAATAGGCAG CTTCAAGACC CTCTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTCAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGACAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGGGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ACAGCAGAGT TGCGCCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTAC TGAAACAAGC TGAGTCTGTG 
ATGCAGGACC TTGGCAGCTC TCGGGCCATG TTAGAAATCC AATATGAAAA TGAGGTTGGT 
ACAGGTCTGG GGCCAACACT GGAGTTTTAT GCACTTGTTT CTCAGGAACT ACAGAGAGCT 
GACTTGTGTC TCTGGAGGGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ACATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCT 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCTCTTG GCCTGCCCTT TTATAAGTGG 
ATGCTGCGGC AGGAAACTTC ATTGACATCT CATGATTTAT TTGACATTGA TCCTGTTGTA 
GCCAGATCAG TATATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT GGAGCAGGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTGGAAA CTCTTACGAT GAATGGCTGT 
TCAGTGGAAG ATCTGGGATT GGATTTTACG CTGCCTGGGT TTCCCAACAT TGAATTGAAG 
AAAGGAGGGA AGGATATTCC AGTTACTATC CACAATTTGG AAGAGTACCT GAGATTGGTG 
ATATTCTGGG CACTAAATGA AGGTGTTTGT CGACAGTTTG ACTCATTCAG AGATGGATTT 
GAATCGGTCT TCCCGCTCTG CCATCTTCAG TACTTCTACC CAGAAGAGCT GGACCAGCTC 
CTGTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAT TGATGGAATG CTGCAGGCCT 
GATCATGGCT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CTGGTAGCCC ACGATTGCCA 
GTTGGAGGAT TTCGGAGTTT AAATCCACCT CTGACAATTG TGCGGAAGAC ATTTGAATCA 
ACAGAAAACC CAGATGACTT CTTACCATCA GTAATGACTT GTGTGAACTA TCTTAAATTG 
CCGGACTACT CGAGCATTGA CATAATGCGG GACAAACTCT TGATAGCAGC AAGAGAAGGC 
CAACAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236967.1
CDS	400..6543
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238665.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238665.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGGTACA AATCAATGAA 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAATC CCTTAGCCAA TTCTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTAATGAA AGAAGATCCG 
GAACTGGCTA AGTCTTTCAT TAAGACCTTG TTTGGTGTTC TCTATGAAGT GTACAGCTCC 
TCAGCAGGCC CTGCGGTCAG ACACAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTT 
GCTGATGCTG AACTTCTGAA GGATGTTCTG AAAAATCATG CTGTTTCAAG TCACATTGCT 
TCAATGCTAT CCAGCCAAGA CCTGAAGATA GTAGTTGGAG CTCTTCAGAT GGCAGAAATC 
CTAATGCAAA AGTTACCTGA TATTTTTAGT GTTTACTTCA GGAGAGAAGG TGTGATGCAT 
CAAGTCAAAC ATTTAGCAGA ATCAGAGTCT TTGTTGACAA GTCCCCCCAA GGCATGTACA 
AATGGGTCAG GATCCCTGGG ATCTACAACA CCAGCCAGCA GTGGGACTGC AACGGCAGCA 
ACCAACGCCT CTGCTGATCT GGGGTCACCT AGCTTGCAGC ATAGCAGAGA TGATTCTTTG 
GATCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTCAAGA GAAAACGACT GCCAAAACGA 
GGGCCAAGAA GGCCAAAATA TTCCCCTCCA AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CCACTACTCA GTCACCTAAA TCTTCCTTCC TGGCAAGCTT GAATCCAAAA 
ACATGGGGAA GGTTAAGCGC ACAGTCCAAC AGCAACAATA TCGAACCAGC ACGAACTGCA 
GGAGTTAGTG GTCTTGCCAG GGCAGCCTCA AAAGACACCA TATCCAATAA TAGAGAAAAA 
ATTAAAGGCT GGATTAAGGA GCAGGCACAT AAATTTGTTG AGCGTTATTT TAGTTCTGAG 
AATATGGATG GAAGCAACCC TGCCTTGAAT GTCCTTCAGA GACTTTGTGC TGCAACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCAG ATGTTTCTTC ATTTGAAATC CAACATAGTG GGTTTGTGAA ACAACTGTTG 
CTGTATTTGA CATCTAAGAA TGAAAAGGAT GCTGTCGGCA GAGAGATCCG GTTAAAACGT 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCTGTTGG AAGAGTAGAA 
CCAGTGGGCC ATGCACCTTT GCTGGCATTG GTTCACAAGA TGAACAACTG TCTCAGCCAA 
ATGGAGCAAT TTCCAGTCAA AGTACATGAT TTCCCTAGTG GGAACGGAGC TGGAGGAAGC 
TTTTCTCTCA ACAGAGGATC ACAAGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGT 
CAGTTACAAA GACACCCAGA CTGTGCAAAT GTGAAGCAGT GGAAAGGCGG GCCTGTCAAG 
ATTGATCCCC TGGCTCTGGT GCAAGCTATT GAGAGATACC TTGTGGTTAG AGGTATTTTC 
CGATATGGAA GAGTAAGAGA AGATGATGAA GATAGTGATG ATGATGGGTC AGATGAAGAA 
ATAGATGAGT CTTTGGCTGC TCAATTCTTA AATTCGGGAA ATGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT ACTACCATAT AACATGACCG TGTATCAGGC AGTGCGGCAA 
TTCAGTGTAC AGGCTGAAGA TGAAAGAGAA TCTACTGATG ATGAGAGCAA TCCTCTGGGA 
AGAGCTGGAA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG GGAGGATGAA 
GAAAGTACTA AAGATTGTGT TGGTGGCAAA AGGGGGAGAG CCCAAACAGC TCCAACAAAA 
ACTTCCCCCA GAAATGCAAA GAAGCATGAT GAGTTATGGC ATGATGGAGT GTGCCCGTCA 
GTAGCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT CACCTTTGAA 
GACCCATCCT TAGATGTAAT ACTTCTTTTA AGAGTTTTAC ATGCCATCAG TCGATATTGG 
TATTACTTGT ATGATAATGC GATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAATTAA CAGCAAAAGC AAATAGGCAG CTTCAAGACC CTCTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTCAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGACAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGGGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ACAGCAGAGT TGCGCCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTAC TGAAACAAGC TGAGTCTGTG 
ATGCAGGACC TTGGCAGCTC TCGGGCCATG TTAGAAATCC AATATGAAAA TGAGGTTGGT 
ACAGGTCTGG GGCCAACACT GGAGTTTTAT GCACTTGTTT CTCAGGAACT ACAGAGAGCT 
GACTTGTGTC TCTGGAGGGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ACATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCT 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCTCTTG GCCTGCCCTT TTATAAGTGG 
ATGCTGCGGC AGGAAACTTC ATTGACATCT CATGATTTAT TTGACATTGA TCCTGTTGTA 
GCCAGATCAG TATATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT GGAGCAGGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTGGAAA CTCTTACGAT GAATGGCTGT 
TCAGTGGAAG ATCTGGGATT GGATTTTACG CTGCCTGGGT TTCCCAACAT TGAATTGAAG 
AAAGGAGGGA AGGATATTCC AGTTACTATC CACAATTTGG AAGAGTACCT GAGATTGGTG 
ATATTCTGGG CACTAAATGA AGGTGTTTGT CGACAGTTTG ACTCATTCAG AGATGGATTT 
GAATCGGTCT TCCCGCTCTG CCATCTTCAG TACTTCTACC CAGAAGAGCT GGACCAGCTC 
CTGTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAT TGATGGAATG CTGCAGGCCT 
GATCATGGCT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CTGGTAGCCC ACGATTGCCA 
GTTGGAGGAT TTCGGAGTTT AAATCCACCT CTGACAATTG TGCGGAAGAC ATTTGAATCA 
ACAGAAAACC CAGATGACTT CTTACCATCA GTAATGACTT GTGTGAACTA TCTTAAATTG 
CCGGACTACT CGAGCATTGA CATAATGCGG GACAAACTCT TGATAGCAGC AAGAGAAGGC 
CAACAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238666.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236968.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X17, mRNA.

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CloneID	OMu67890
Clone ID Related Accession (Same CDS sequence)	XM_011238666.2 , XM_011238666.3
Accession Version	XM_011238666.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238666.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGCAAAT CAATGAAGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAATCCCT TAGCCAATTC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGATCCGGAA 
CTGGCTAAGT CTTTCATTAA GACCTTGTTT GGTGTTCTCT ATGAAGTGTA CAGCTCCTCA 
GCAGGCCCTG CGGTCAGACA CAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTTGCT 
GATGCTGAAC TTCTGAAGGA TGTTCTGAAA AATCATGCTG TTTCAAGTCA CATTGCTTCA 
ATGCTATCCA GCCAAGACCT GAAGATAGTA GTTGGAGCTC TTCAGATGGC AGAAATCCTA 
ATGCAAAAGT TACCTGATAT TTTTAGTGTT TACTTCAGGA GAGAAGGTGT GATGCATCAA 
GTCAAACATT TAGCAGAATC AGAGTCTTTG TTGACAAGTC CCCCCAAGGC ATGTACAAAT 
GGGTCAGGAT CCCTGGGATC TACAACACCA GCCAGCAGTG GGACTGCAAC GGCAGCAACC 
AACGCCTCTG CTGATCTGGG GTCACCTAGC TTGCAGCATA GCAGAGATGA TTCTTTGGAT 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTCAAGAGAA AACGACTGCC AAAACGAGGG 
CCAAGAAGGC CAAAATATTC CCCTCCAAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAATCT TCCTTCCTGG CAAGCTTGAA TCCAAAAACA 
TGGGGAAGGT TAAGCGCACA GTCCAACAGC AACAATATCG AACCAGCACG AACTGCAGGA 
GTTAGTGGTC TTGCCAGGGC AGCCTCAAAA GACACCATAT CCAATAATAG AGAAAAAATT 
AAAGGCTGGA TTAAGGAGCA GGCACATAAA TTTGTTGAGC GTTATTTTAG TTCTGAGAAT 
ATGGATGGAA GCAACCCTGC CTTGAATGTC CTTCAGAGAC TTTGTGCTGC AACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCAGATG TTTCTTCATT TGAAATCCAA CATAGTGGGT TTGTGAAACA ACTGTTGCTG 
TATTTGACAT CTAAGAATGA AAAGGATGCT GTCGGCAGAG AGATCCGGTT AAAACGTTTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CTGTTGGAAG AGTAGAACCA 
GTGGGCCATG CACCTTTGCT GGCATTGGTT CACAAGATGA ACAACTGTCT CAGCCAAATG 
GAGCAATTTC CAGTCAAAGT ACATGATTTC CCTAGTGGGA ACGGAGCTGG AGGAAGCTTT 
TCTCTCAACA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG TATTTTCCGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236968.1
CDS	400..6540
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238666.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238666.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG CTTCAGCCCC AAAGTTAGCC TACCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAGTC CACCAAGAAA CGCAGTGAGT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGA GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAGAG AGGAGCAGCT GAGGCCCGGC GGCAAGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGAC AGTAAATTCC TCAGCTGCCC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCGGATG ATTCTGAGAT GGGACGATTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCT 
CCTCACCTAT TTGGTCCTCT CGGGCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GTTCTAAGGC CCAGCAGCTT CTTCAAGGAC TACAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCCGTCAT TGAAATGTGT CAGTTATTGG TCATGGGGAA TGAGGAGACA 
CTAGGAGGGT TTCCTGTGAA GAGTGTTGTT CCAGCTTTGA TAACATTACT GCAGATGGAA 
CATAATTTTG ACATTATGAA TCATGCTTGC CGAGCCTTAA CATATATGAT GGAAGCTCTC 
CCCCGGTCAT CTGCTGTTGT AGTGGATGCT ATCCCTGTTT TCTTAGAAAA GTTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGCGA 
CACAGTAAAG CCATTTTACA AGCGGGTGGT TTGGCAGATT GCTTGTTGTA TCTAGAATTC 
TTCAGCATCA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACACCAGATG AGTTTCATTT TGTGGCAGAT TCCCTACCAT TGCTTACCCA AAGGCTAACT 
CATCAGGACA AAAAGTCAGT AGAAAGTACT TGCCTTTGTT TTGCACGTTT AGTGGACAAC 
TTTCAACATG AGGAGAACTT ACTGCAACAG GTTGCTTCCA AAGATCTACT TACAAATGTT 
CAACAATTGT TGGTAGTGAC TCCACCGATT TTAAGTTCTG GGATGTTTAT TATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACATTAG CTGTTCAACT TATGAAACAA 
AGCTTTACAG ACATTGCAGA AACACTTCAC TTCCTGCTAT GTGGTGCCTC CAATGGAAGT 
TGTCAAGAGC AGATTGACCT TGTTCCACGA AGTCCTCAAG AGCTGTATGA GCTGACATCT 
TTGATTTGTG AACTTATGCC ATGTTTACCA AAAGAAGGCA TCTTCGCAGT TGATACCATG 
TTGAAAAAGG GAAATGCACA GAACACAGAT GGTGCAATAT GGCAGTGGCG GGATGACCGA 
GGCCTCTGGC ATCCCTATAA CAGGATTGAC AGCCGGATCA TTGAGCAAAT CAATGAAGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAATCCCT TAGCCAATTC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGATCCGGAA 
CTGGCTAAGT CTTTCATTAA GACCTTGTTT GGTGTTCTCT ATGAAGTGTA CAGCTCCTCA 
GCAGGCCCTG CGGTCAGACA CAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTTGCT 
GATGCTGAAC TTCTGAAGGA TGTTCTGAAA AATCATGCTG TTTCAAGTCA CATTGCTTCA 
ATGCTATCCA GCCAAGACCT GAAGATAGTA GTTGGAGCTC TTCAGATGGC AGAAATCCTA 
ATGCAAAAGT TACCTGATAT TTTTAGTGTT TACTTCAGGA GAGAAGGTGT GATGCATCAA 
GTCAAACATT TAGCAGAATC AGAGTCTTTG TTGACAAGTC CCCCCAAGGC ATGTACAAAT 
GGGTCAGGAT CCCTGGGATC TACAACACCA GCCAGCAGTG GGACTGCAAC GGCAGCAACC 
AACGCCTCTG CTGATCTGGG GTCACCTAGC TTGCAGCATA GCAGAGATGA TTCTTTGGAT 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTCAAGAGAA AACGACTGCC AAAACGAGGG 
CCAAGAAGGC CAAAATATTC CCCTCCAAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAATCT TCCTTCCTGG CAAGCTTGAA TCCAAAAACA 
TGGGGAAGGT TAAGCGCACA GTCCAACAGC AACAATATCG AACCAGCACG AACTGCAGGA 
GTTAGTGGTC TTGCCAGGGC AGCCTCAAAA GACACCATAT CCAATAATAG AGAAAAAATT 
AAAGGCTGGA TTAAGGAGCA GGCACATAAA TTTGTTGAGC GTTATTTTAG TTCTGAGAAT 
ATGGATGGAA GCAACCCTGC CTTGAATGTC CTTCAGAGAC TTTGTGCTGC AACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCAGATG TTTCTTCATT TGAAATCCAA CATAGTGGGT TTGTGAAACA ACTGTTGCTG 
TATTTGACAT CTAAGAATGA AAAGGATGCT GTCGGCAGAG AGATCCGGTT AAAACGTTTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CTGTTGGAAG AGTAGAACCA 
GTGGGCCATG CACCTTTGCT GGCATTGGTT CACAAGATGA ACAACTGTCT CAGCCAAATG 
GAGCAATTTC CAGTCAAAGT ACATGATTTC CCTAGTGGGA ACGGAGCTGG AGGAAGCTTT 
TCTCTCAACA GAGGATCACA AGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGTCAG 
TTACAAAGAC ACCCAGACTG TGCAAATGTG AAGCAGTGGA AAGGCGGGCC TGTCAAGATT 
GATCCCCTGG CTCTGGTGCA AGCTATTGAG AGATACCTTG TGGTTAGAGG TATTTTCCGA 
TATGGAAGAG TAAGAGAAGA TGATGAAGAT AGTGATGATG ATGGGTCAGA TGAAGAAATA 
GATGAGTCTT TGGCTGCTCA ATTCTTAAAT TCGGGAAATG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTACT ACCATATAAC ATGACCGTGT ATCAGGCAGT GCGGCAATTC 
AGTGTACAGG CTGAAGATGA AAGAGAATCT ACTGATGATG AGAGCAATCC TCTGGGAAGA 
GCTGGAATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGGGA GGATGAAGAA 
AGTACTAAAG ATTGTGTTGG TGGCAAAAGG GGGAGAGCCC AAACAGCTCC AACAAAAACT 
TCCCCCAGAA ATGCAAAGAA GCATGATGAG TTATGGCATG ATGGAGTGTG CCCGTCAGTA 
GCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATCAC CTTTGAAGAC 
CCATCCTTAG ATGTAATACT TCTTTTAAGA GTTTTACATG CCATCAGTCG ATATTGGTAT 
TACTTGTATG ATAATGCGAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAATTAACAG CAAAAGCAAA TAGGCAGCTT CAAGACCCTC TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTCACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGACAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GGGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGACA GCAGAGTTGC GCCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTACTGA AACAAGCTGA GTCTGTGATG 
CAGGACCTTG GCAGCTCTCG GGCCATGTTA GAAATCCAAT ATGAAAATGA GGTTGGTACA 
GGTCTGGGGC CAACACTGGA GTTTTATGCA CTTGTTTCTC AGGAACTACA GAGAGCTGAC 
TTGTGTCTCT GGAGGGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCACA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCTAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCTCTTGGCC TGCCCTTTTA TAAGTGGATG 
CTGCGGCAGG AAACTTCATT GACATCTCAT GATTTATTTG ACATTGATCC TGTTGTAGCC 
AGATCAGTAT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTGGA GCAGGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTGGAAACTC TTACGATGAA TGGCTGTTCA 
GTGGAAGATC TGGGATTGGA TTTTACGCTG CCTGGGTTTC CCAACATTGA ATTGAAGAAA 
GGAGGGAAGG ATATTCCAGT TACTATCCAC AATTTGGAAG AGTACCTGAG ATTGGTGATA 
TTCTGGGCAC TAAATGAAGG TGTTTGTCGA CAGTTTGACT CATTCAGAGA TGGATTTGAA 
TCGGTCTTCC CGCTCTGCCA TCTTCAGTAC TTCTACCCAG AAGAGCTGGA CCAGCTCCTG 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACATTGA TGGAATGCTG CAGGCCTGAT 
CATGGCTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACTG GTAGCCCACG ATTGCCAGTT 
GGAGGATTTC GGAGTTTAAA TCCACCTCTG ACAATTGTGC GGAAGACATT TGAATCAACA 
GAAAACCCAG ATGACTTCTT ACCATCAGTA ATGACTTGTG TGAACTATCT TAAATTGCCG 
GACTACTCGA GCATTGACAT AATGCGGGAC AAACTCTTGA TAGCAGCAAG AGAAGGCCAA 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238667.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236969.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X19, mRNA.

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CloneID	OMu67891
Clone ID Related Accession (Same CDS sequence)	XM_011238667.3 , XM_011238667.2
Accession Version	XM_011238667.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA GTCCACCAAG 
AAACGCAGTG AGTCCCCACC TGCTGAGCTC CCCAGTTTGA GGAGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA GAGAGGAGCA 
GCTGAGGCCC GGCGGCAAGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GACAGTAAAT 
TCCTCAGCTG CCCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCGG ATGATTCTGA GATGGGACGA 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCTCCTCACC TATTTGGTCC TCTCGGGCCT 
CGGATGTCAC AACTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTTCAAG GACTACAAGC CAGTGATGAA AGTCAACAGC TTCAGGCCGT CATTGAAATG 
TGTCAGTTAT TGGTCATGGG GAATGAGGAG ACACTAGGAG GGTTTCCTGT GAAGAGTGTT 
GTTCCAGCTT TGATAACATT ACTGCAGATG GAACATAATT TTGACATTAT GAATCATGCT 
TGCCGAGCCT TAACATATAT GATGGAAGCT CTCCCCCGGT CATCTGCTGT TGTAGTGGAT 
GCTATCCCTG TTTTCTTAGA AAAGTTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG CGACACAGTA AAGCCATTTT ACAAGCGGGT 
GGTTTGGCAG ATTGCTTGTT GTATCTAGAA TTCTTCAGCA TCAATGCCCA AAGAAATGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACACCAG ATGAGTTTCA TTTTGTGGCA 
GATTCCCTAC CATTGCTTAC CCAAAGGCTA ACTCATCAGG ACAAAAAGTC AGTAGAAAGT 
ACTTGCCTTT GTTTTGCACG TTTAGTGGAC AACTTTCAAC ATGAGGAGAA CTTACTGCAA 
CAGGTTGCTT CCAAAGATCT ACTTACAAAT GTTCAACAAT TGTTGGTAGT GACTCCACCG 
ATTTTAAGTT CTGGGATGTT TATTATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACAT TAGCTGTTCA ACTTATGAAA CAAAGCTTTA CAGACATTGC AGAAACACTT 
CACTTCCTGC TATGTGGTGC CTCCAATGGA AGTTGTCAAG AGCAGATTGA CCTTGTTCCA 
CGAAGTCCTC AAGAGCTGTA TGAGCTGACA TCTTTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAAGAAG GCATCTTCGC AGTTGATACC ATGTTGAAAA AGGGAAATGC ACAGAACACA 
GATGGTGCAA TATGGCAGTG GCGGGATGAC CGAGGCCTCT GGCATCCCTA TAACAGGATT 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAAGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AATCCCTTAG CCAATTCTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTAA TGAAAGAAGA TCCGGAACTG 
GCTAAGTCTT TCATTAAGAC CTTGTTTGGT GTTCTCTATG AAGTGTACAG CTCCTCAGCA 
GGCCCTGCGG TCAGACACAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTTGCTGAT 
GCTGAACTTC TGAAGGATGT TCTGAAAAAT CATGCTGTTT CAAGTCACAT TGCTTCAATG 
CTATCCAGCC AAGACCTGAA GATAGTAGTT GGAGCTCTTC AGATGGCAGA AATCCTAATG 
CAAAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGGAGAG AAGGTGTGAT GCATCAAGTC 
AAACATTTAG CAGAATCAGA GTCTTTGTTG ACAAGTCCCC CCAAGGCATG TACAAATGGG 
TCAGGATCCC TGGGATCTAC AACACCAGCC AGCAGTGGGA CTGCAACGGC AGCAACCAAC 
GCCTCTGCTG ATCTGGGGTC ACCTAGCTTG CAGCATAGCA GAGATGATTC TTTGGATCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTC AAGAGAAAAC GACTGCCAAA ACGAGGGCCA 
AGAAGGCCAA AATATTCCCC TCCAAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAATCTTCC TTCCTGGCAA GCTTGAATCC AAAAACATGG 
GGAAGGTTAA GCGCACAGTC CAACAGCAAC AATATCGAAC CAGCACGAAC TGCAGGAGTT 
AGTGGTCTTG CCAGGGCAGC CTCAAAAGAC ACCATATCCA ATAATAGAGA AAAAATTAAA 
GGCTGGATTA AGGAGCAGGC ACATAAATTT GTTGAGCGTT ATTTTAGTTC TGAGAATATG 
GATGGAAGCA ACCCTGCCTT GAATGTCCTT CAGAGACTTT GTGCTGCAAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCAGATGTTT CTTCATTTGA AATCCAACAT AGTGGGTTTG TGAAACAACT GTTGCTGTAT 
TTGACATCTA AGAATGAAAA GGATGCTGTC GGCAGAGAGA TCCGGTTAAA ACGTTTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCTG TTGGAAGAGT AGAACCAGTG 
GGCCATGCAC CTTTGCTGGC ATTGGTTCAC AAGATGAACA ACTGTCTCAG CCAAATGGAG 
CAATTTCCAG TCAAAGTACA TGATTTCCCT AGTGGGAACG GAGCTGGAGG AAGCTTTTCT 
CTCAACAGAG GATCACAAGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGTCAGTTA 
CAAAGACACC CAGACTGTGC AAATGTGAAG CAGTGGAAAG GCGGGCCTGT CAAGATTGAT 
CCCCTGGCTC TGGTGCAAGC TATTGAGAGA TACCTTGTGG TTAGAGGTAT TTTCCGATAT 
GGAAGAGTAA GAGAAGATGA TGAAGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAGTCTTTGG CTGCTCAATT CTTAAATTCG GGAAATGTAA GGCACAGGCT ACAGTTTTAT 
ATTGGAGAAC ATTTACTACC ATATAACATG ACCGTGTATC AGGCAGTGCG GCAATTCAGT 
GTACAGGCTG AAGATGAAAG AGAATCTACT GATGATGAGA GCAATCCTCT GGGAAGAGCT 
GGAATTTGGA CAAAGACTCA TACAATATGG TACAAACCTG TGAGGGAGGA TGAAGAAAGT 
ACTAAAGATT GTGTTGGTGG CAAAAGGGGG AGAGCCCAAA CAGCTCCAAC AAAAACTTCC 
CCCAGAAATG CAAAGAAGCA TGATGAGTTA TGGCATGATG GAGTGTGCCC GTCAGTAGCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATCACCTT TGAAGACCCA 
TCCTTAGATG TAATACTTCT TTTAAGAGTT TTACATGCCA TCAGTCGATA TTGGTATTAC 
TTGTATGATA ATGCGATGTG CAAGGAGATT ATTCCAACTA GTGAATTTAT TAACAGTAAA 
TTAACAGCAA AAGCAAATAG GCAGCTTCAA GACCCTCTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TCACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGA 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGGG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGACAGCA GAGTTGCGCC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTACTGAAAC AAGCTGAGTC TGTGATGCAG 
GACCTTGGCA GCTCTCGGGC CATGTTAGAA ATCCAATATG AAAATGAGGT TGGTACAGGT 
CTGGGGCCAA CACTGGAGTT TTATGCACTT GTTTCTCAGG AACTACAGAG AGCTGACTTG 
TGTCTCTGGA GGGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCACATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCTAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCT CTTGGCCTGC CCTTTTATAA GTGGATGCTG 
CGGCAGGAAA CTTCATTGAC ATCTCATGAT TTATTTGACA TTGATCCTGT TGTAGCCAGA 
TCAGTATATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTGGAGCA GGATAAATCC 
CAGACCAAAG AGAGTCTACA ATATGCATTG GAAACTCTTA CGATGAATGG CTGTTCAGTG 
GAAGATCTGG GATTGGATTT TACGCTGCCT GGGTTTCCCA ACATTGAATT GAAGAAAGGA 
GGGAAGGATA TTCCAGTTAC TATCCACAAT TTGGAAGAGT ACCTGAGATT GGTGATATTC 
TGGGCACTAA ATGAAGGTGT TTGTCGACAG TTTGACTCAT TCAGAGATGG ATTTGAATCG 
GTCTTCCCGC TCTGCCATCT TCAGTACTTC TACCCAGAAG AGCTGGACCA GCTCCTGTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACATTGATGG AATGCTGCAG GCCTGATCAT 
GGCTATACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTGTT TCTCCAGTTT GTGACTGGTA GCCCACGATT GCCAGTTGGA 
GGATTTCGGA GTTTAAATCC ACCTCTGACA ATTGTGCGGA AGACATTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTACC ATCAGTAATG ACTTGTGTGA ACTATCTTAA ATTGCCGGAC 
TACTCGAGCA TTGACATAAT GCGGGACAAA CTCTTGATAG CAGCAAGAGA AGGCCAACAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236969.1
CDS	400..6537
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKRKGYIPPESRKSNFKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238667.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238667.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACACTTAGG GCAGGCAAAA 
CGTAAGGGAT ATATCCCTCC TGAGAGTAGA AAATCTAATT TTAAGGCACC CAAAGTGCAA 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAG 
AAAACGGGGC AGGTGCCTAA AAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCGAAAAAGC CCAGAGCATT TCAGCATATT 
GAGTCTTTCT CAGAAACCAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATTGCCG TCAACAAGCA AGGCTCACAC CAGAAAGAGT 
GTGGCTGCTG GTAGTTCACG GAATCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTGTTAAA 
AGTGGTTCTG GGTCCGAATC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CATCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCATC ATCTTCAGCT ATAGCCTCGG CCTCTTCTAC TGTACCAGCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGCCCTAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA GTCCACCAAG 
AAACGCAGTG AGTCCCCACC TGCTGAGCTC CCCAGTTTGA GGAGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA GAGAGGAGCA 
GCTGAGGCCC GGCGGCAAGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GACAGTAAAT 
TCCTCAGCTG CCCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCGG ATGATTCTGA GATGGGACGA 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCTCCTCACC TATTTGGTCC TCTCGGGCCT 
CGGATGTCAC AACTTTTCCA TAGAACAATT GGAAGCGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTTCAAG GACTACAAGC CAGTGATGAA AGTCAACAGC TTCAGGCCGT CATTGAAATG 
TGTCAGTTAT TGGTCATGGG GAATGAGGAG ACACTAGGAG GGTTTCCTGT GAAGAGTGTT 
GTTCCAGCTT TGATAACATT ACTGCAGATG GAACATAATT TTGACATTAT GAATCATGCT 
TGCCGAGCCT TAACATATAT GATGGAAGCT CTCCCCCGGT CATCTGCTGT TGTAGTGGAT 
GCTATCCCTG TTTTCTTAGA AAAGTTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG CGACACAGTA AAGCCATTTT ACAAGCGGGT 
GGTTTGGCAG ATTGCTTGTT GTATCTAGAA TTCTTCAGCA TCAATGCCCA AAGAAATGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACACCAG ATGAGTTTCA TTTTGTGGCA 
GATTCCCTAC CATTGCTTAC CCAAAGGCTA ACTCATCAGG ACAAAAAGTC AGTAGAAAGT 
ACTTGCCTTT GTTTTGCACG TTTAGTGGAC AACTTTCAAC ATGAGGAGAA CTTACTGCAA 
CAGGTTGCTT CCAAAGATCT ACTTACAAAT GTTCAACAAT TGTTGGTAGT GACTCCACCG 
ATTTTAAGTT CTGGGATGTT TATTATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACAT TAGCTGTTCA ACTTATGAAA CAAAGCTTTA CAGACATTGC AGAAACACTT 
CACTTCCTGC TATGTGGTGC CTCCAATGGA AGTTGTCAAG AGCAGATTGA CCTTGTTCCA 
CGAAGTCCTC AAGAGCTGTA TGAGCTGACA TCTTTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAAGAAG GCATCTTCGC AGTTGATACC ATGTTGAAAA AGGGAAATGC ACAGAACACA 
GATGGTGCAA TATGGCAGTG GCGGGATGAC CGAGGCCTCT GGCATCCCTA TAACAGGATT 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAAGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AATCCCTTAG CCAATTCTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTAA TGAAAGAAGA TCCGGAACTG 
GCTAAGTCTT TCATTAAGAC CTTGTTTGGT GTTCTCTATG AAGTGTACAG CTCCTCAGCA 
GGCCCTGCGG TCAGACACAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTTGCTGAT 
GCTGAACTTC TGAAGGATGT TCTGAAAAAT CATGCTGTTT CAAGTCACAT TGCTTCAATG 
CTATCCAGCC AAGACCTGAA GATAGTAGTT GGAGCTCTTC AGATGGCAGA AATCCTAATG 
CAAAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGGAGAG AAGGTGTGAT GCATCAAGTC 
AAACATTTAG CAGAATCAGA GTCTTTGTTG ACAAGTCCCC CCAAGGCATG TACAAATGGG 
TCAGGATCCC TGGGATCTAC AACACCAGCC AGCAGTGGGA CTGCAACGGC AGCAACCAAC 
GCCTCTGCTG ATCTGGGGTC ACCTAGCTTG CAGCATAGCA GAGATGATTC TTTGGATCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTC AAGAGAAAAC GACTGCCAAA ACGAGGGCCA 
AGAAGGCCAA AATATTCCCC TCCAAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAATCTTCC TTCCTGGCAA GCTTGAATCC AAAAACATGG 
GGAAGGTTAA GCGCACAGTC CAACAGCAAC AATATCGAAC CAGCACGAAC TGCAGGAGTT 
AGTGGTCTTG CCAGGGCAGC CTCAAAAGAC ACCATATCCA ATAATAGAGA AAAAATTAAA 
GGCTGGATTA AGGAGCAGGC ACATAAATTT GTTGAGCGTT ATTTTAGTTC TGAGAATATG 
GATGGAAGCA ACCCTGCCTT GAATGTCCTT CAGAGACTTT GTGCTGCAAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCAGATGTTT CTTCATTTGA AATCCAACAT AGTGGGTTTG TGAAACAACT GTTGCTGTAT 
TTGACATCTA AGAATGAAAA GGATGCTGTC GGCAGAGAGA TCCGGTTAAA ACGTTTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCTG TTGGAAGAGT AGAACCAGTG 
GGCCATGCAC CTTTGCTGGC ATTGGTTCAC AAGATGAACA ACTGTCTCAG CCAAATGGAG 
CAATTTCCAG TCAAAGTACA TGATTTCCCT AGTGGGAACG GAGCTGGAGG AAGCTTTTCT 
CTCAACAGAG GATCACAAGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGTCAGTTA 
CAAAGACACC CAGACTGTGC AAATGTGAAG CAGTGGAAAG GCGGGCCTGT CAAGATTGAT 
CCCCTGGCTC TGGTGCAAGC TATTGAGAGA TACCTTGTGG TTAGAGGTAT TTTCCGATAT 
GGAAGAGTAA GAGAAGATGA TGAAGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAGTCTTTGG CTGCTCAATT CTTAAATTCG GGAAATGTAA GGCACAGGCT ACAGTTTTAT 
ATTGGAGAAC ATTTACTACC ATATAACATG ACCGTGTATC AGGCAGTGCG GCAATTCAGT 
GTACAGGCTG AAGATGAAAG AGAATCTACT GATGATGAGA GCAATCCTCT GGGAAGAGCT 
GGAATTTGGA CAAAGACTCA TACAATATGG TACAAACCTG TGAGGGAGGA TGAAGAAAGT 
ACTAAAGATT GTGTTGGTGG CAAAAGGGGG AGAGCCCAAA CAGCTCCAAC AAAAACTTCC 
CCCAGAAATG CAAAGAAGCA TGATGAGTTA TGGCATGATG GAGTGTGCCC GTCAGTAGCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATCACCTT TGAAGACCCA 
TCCTTAGATG TAATACTTCT TTTAAGAGTT TTACATGCCA TCAGTCGATA TTGGTATTAC 
TTGTATGATA ATGCGATGTG CAAGGAGATT ATTCCAACTA GTGAATTTAT TAACAGTAAA 
TTAACAGCAA AAGCAAATAG GCAGCTTCAA GACCCTCTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TCACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGA 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGGG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGACAGCA GAGTTGCGCC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTACTGAAAC AAGCTGAGTC TGTGATGCAG 
GACCTTGGCA GCTCTCGGGC CATGTTAGAA ATCCAATATG AAAATGAGGT TGGTACAGGT 
CTGGGGCCAA CACTGGAGTT TTATGCACTT GTTTCTCAGG AACTACAGAG AGCTGACTTG 
TGTCTCTGGA GGGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCACATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCTAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCT CTTGGCCTGC CCTTTTATAA GTGGATGCTG 
CGGCAGGAAA CTTCATTGAC ATCTCATGAT TTATTTGACA TTGATCCTGT TGTAGCCAGA 
TCAGTATATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTGGAGCA GGATAAATCC 
CAGACCAAAG AGAGTCTACA ATATGCATTG GAAACTCTTA CGATGAATGG CTGTTCAGTG 
GAAGATCTGG GATTGGATTT TACGCTGCCT GGGTTTCCCA ACATTGAATT GAAGAAAGGA 
GGGAAGGATA TTCCAGTTAC TATCCACAAT TTGGAAGAGT ACCTGAGATT GGTGATATTC 
TGGGCACTAA ATGAAGGTGT TTGTCGACAG TTTGACTCAT TCAGAGATGG ATTTGAATCG 
GTCTTCCCGC TCTGCCATCT TCAGTACTTC TACCCAGAAG AGCTGGACCA GCTCCTGTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACATTGATGG AATGCTGCAG GCCTGATCAT 
GGCTATACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTGTT TCTCCAGTTT GTGACTGGTA GCCCACGATT GCCAGTTGGA 
GGATTTCGGA GTTTAAATCC ACCTCTGACA ATTGTGCGGA AGACATTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTACC ATCAGTAATG ACTTGTGTGA ACTATCTTAA ATTGCCGGAC 
TACTCGAGCA TTGACATAAT GCGGGACAAA CTCTTGATAG CAGCAAGAGA AGGCCAACAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238671.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236973.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X25, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_011238671.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236973.1
CDS	498..6596
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238671.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238671.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238672.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236974.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X26, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_011238672.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236974.1
CDS	115..6213
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238672.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238672.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238669.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236971.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X23, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_011238669.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236971.1
CDS	125..6223
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238669.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238669.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238668.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236970.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X22, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_011238668.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238668.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236970.1
CDS	400..6498
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238668.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238668.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238670.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236972.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X24, mRNA.

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CloneID	OMu67892
Clone ID Related Accession (Same CDS sequence)	XM_011238671.2 , XM_011238672.2 , XM_011238669.3 , XM_011238669.2 , XM_011238670.3 , XM_011238671.3 , XM_030249149.1 , XM_011238668.2 , XM_011238672.3 , XM_011238670.2 , XM_011238668.3 , XM_030249155.1
Accession Version	XM_011238670.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236972.1
CDS	259..6357
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238670.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238670.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAGCTTT 
ACAGACATTG CAGAAACACT TCACTTCCTG CTATGTGGTG CCTCCAATGG AAGTTGTCAA 
GAGCAGATTG ACCTTGTTCC ACGAAGTCCT CAAGAGCTGT ATGAGCTGAC ATCTTTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAAGAA GGCATCTTCG CAGTTGATAC CATGTTGAAA 
AAGGGAAATG CACAGAACAC AGATGGTGCA ATATGGCAGT GGCGGGATGA CCGAGGCCTC 
TGGCATCCCT ATAACAGGAT TGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAAGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAATCCCTTA 
GCCAATTCTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ATCCGGAACT GGCTAAGTCT TTCATTAAGA CCTTGTTTGG TGTTCTCTAT 
GAAGTGTACA GCTCCTCAGC AGGCCCTGCG GTCAGACACA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTTGCTGA TGCTGAACTT CTGAAGGATG TTCTGAAAAA TCATGCTGTT 
TCAAGTCACA TTGCTTCAAT GCTATCCAGC CAAGACCTGA AGATAGTAGT TGGAGCTCTT 
CAGATGGCAG AAATCCTAAT GCAAAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGGAGA 
GAAGGTGTGA TGCATCAAGT CAAACATTTA GCAGAATCAG AGTCTTTGTT GACAAGTCCC 
CCCAAGGCAT GTACAAATGG GTCAGGATCC CTGGGATCTA CAACACCAGC CAGCAGTGGG 
ACTGCAACGG CAGCAACCAA CGCCTCTGCT GATCTGGGGT CACCTAGCTT GCAGCATAGC 
AGAGATGATT CTTTGGATCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT CAAGAGAAAA 
CGACTGCCAA AACGAGGGCC AAGAAGGCCA AAATATTCCC CTCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAATCTTC CTTCCTGGCA 
AGCTTGAATC CAAAAACATG GGGAAGGTTA AGCGCACAGT CCAACAGCAA CAATATCGAA 
CCAGCACGAA CTGCAGGAGT TAGTGGTCTT GCCAGGGCAG CCTCAAAAGA CACCATATCC 
AATAATAGAG AAAAAATTAA AGGCTGGATT AAGGAGCAGG CACATAAATT TGTTGAGCGT 
TATTTTAGTT CTGAGAATAT GGATGGAAGC AACCCTGCCT TGAATGTCCT TCAGAGACTT 
TGTGCTGCAA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCAGATGTT TCTTCATTTG AAATCCAACA TAGTGGGTTT 
GTGAAACAAC TGTTGCTGTA TTTGACATCT AAGAATGAAA AGGATGCTGT CGGCAGAGAG 
ATCCGGTTAA AACGTTTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCT 
GTTGGAAGAG TAGAACCAGT GGGCCATGCA CCTTTGCTGG CATTGGTTCA CAAGATGAAC 
AACTGTCTCA GCCAAATGGA GCAATTTCCA GTCAAAGTAC ATGATTTCCC TAGTGGGAAC 
GGAGCTGGAG GAAGCTTTTC TCTCAACAGA GGATCACAAG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGTCAGTT ACAAAGACAC CCAGACTGTG CAAATGTGAA GCAGTGGAAA 
GGCGGGCCTG TCAAGATTGA TCCCCTGGCT CTGGTGCAAG CTATTGAGAG ATACCTTGTG 
GTTAGAGGTA TTTTCCGATA TGGAAGAGTA AGAGAAGATG ATGAAGATAG TGATGATGAT 
GGGTCAGATG AAGAAATAGA TGAGTCTTTG GCTGCTCAAT TCTTAAATTC GGGAAATGTA 
AGGCACAGGC TACAGTTTTA TATTGGAGAA CATTTACTAC CATATAACAT GACCGTGTAT 
CAGGCAGTGC GGCAATTCAG TGTACAGGCT GAAGATGAAA GAGAATCTAC TGATGATGAG 
AGCAATCCTC TGGGAAGAGC TGGAATTTGG ACAAAGACTC ATACAATATG GTACAAACCT 
GTGAGGGAGG ATGAAGAAAG TACTAAAGAT TGTGTTGGTG GCAAAAGGGG GAGAGCCCAA 
ACAGCTCCAA CAAAAACTTC CCCCAGAAAT GCAAAGAAGC ATGATGAGTT ATGGCATGAT 
GGAGTGTGCC CGTCAGTAGC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATCACCT TTGAAGACCC ATCCTTAGAT GTAATACTTC TTTTAAGAGT TTTACATGCC 
ATCAGTCGAT ATTGGTATTA CTTGTATGAT AATGCGATGT GCAAGGAGAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA ATTAACAGCA AAAGCAAATA GGCAGCTTCA AGACCCTCTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTCACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG ACAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGG 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGACAGC 
AGAGTTGCGC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTACTGAAA 
CAAGCTGAGT CTGTGATGCA GGACCTTGGC AGCTCTCGGG CCATGTTAGA AATCCAATAT 
GAAAATGAGG TTGGTACAGG TCTGGGGCCA ACACTGGAGT TTTATGCACT TGTTTCTCAG 
GAACTACAGA GAGCTGACTT GTGTCTCTGG AGGGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCACATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCTAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC TCTTGGCCTG 
CCCTTTTATA AGTGGATGCT GCGGCAGGAA ACTTCATTGA CATCTCATGA TTTATTTGAC 
ATTGATCCTG TTGTAGCCAG ATCAGTATAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTGGAGC AGGATAAATC CCAGACCAAA GAGAGTCTAC AATATGCATT GGAAACTCTT 
ACGATGAATG GCTGTTCAGT GGAAGATCTG GGATTGGATT TTACGCTGCC TGGGTTTCCC 
AACATTGAAT TGAAGAAAGG AGGGAAGGAT ATTCCAGTTA CTATCCACAA TTTGGAAGAG 
TACCTGAGAT TGGTGATATT CTGGGCACTA AATGAAGGTG TTTGTCGACA GTTTGACTCA 
TTCAGAGATG GATTTGAATC GGTCTTCCCG CTCTGCCATC TTCAGTACTT CTACCCAGAA 
GAGCTGGACC AGCTCCTGTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACATTGATG 
GAATGCTGCA GGCCTGATCA TGGCTATACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTGT TTCTCCAGTT TGTGACTGGT 
AGCCCACGAT TGCCAGTTGG AGGATTTCGG AGTTTAAATC CACCTCTGAC AATTGTGCGG 
AAGACATTTG AATCAACAGA AAACCCAGAT GACTTCTTAC CATCAGTAAT GACTTGTGTG 
AACTATCTTA AATTGCCGGA CTACTCGAGC ATTGACATAA TGCGGGACAA ACTCTTGATA 
GCAGCAAGAG AAGGCCAACA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trip12 ( XM_011238673.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236975.1 Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X27, mRNA.

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CloneID	OMu67893
Clone ID Related Accession (Same CDS sequence)	XM_011238673.2 , XM_030249195.1 , XM_011238673.3 , XM_030249168.1 , XM_030249180.1
Accession Version	XM_011238673.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAAGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAATCCCTT AGCCAATTCT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT AATGAAAGAA 
GATCCGGAAC TGGCTAAGTC TTTCATTAAG ACCTTGTTTG GTGTTCTCTA TGAAGTGTAC 
AGCTCCTCAG CAGGCCCTGC GGTCAGACAC AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCTG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCAA TGCTATCCAG CCAAGACCTG AAGATAGTAG TTGGAGCTCT TCAGATGGCA 
GAAATCCTAA TGCAAAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGGAG AGAAGGTGTG 
ATGCATCAAG TCAAACATTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC CCCCAAGGCA 
TGTACAAATG GGTCAGGATC CCTGGGATCT ACAACACCAG CCAGCAGTGG GACTGCAACG 
GCAGCAACCA ACGCCTCTGC TGATCTGGGG TCACCTAGCT TGCAGCATAG CAGAGATGAT 
TCTTTGGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TCAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAATATTCC CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CCTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAATATCGA ACCAGCACGA 
ACTGCAGGAG TTAGTGGTCT TGCCAGGGCA GCCTCAAAAG ACACCATATC CAATAATAGA 
GAAAAAATTA AAGGCTGGAT TAAGGAGCAG GCACATAAAT TTGTTGAGCG TTATTTTAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCC TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCTTCATTT GAAATCCAAC ATAGTGGGTT TGTGAAACAA 
CTGTTGCTGT ATTTGACATC TAAGAATGAA AAGGATGCTG TCGGCAGAGA GATCCGGTTA 
AAACGTTTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC TGTTGGAAGA 
GTAGAACCAG TGGGCCATGC ACCTTTGCTG GCATTGGTTC ACAAGATGAA CAACTGTCTC 
AGCCAAATGG AGCAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGGAA CGGAGCTGGA 
GGAAGCTTTT CTCTCAACAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236975.1
CDS	114..6203
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGIFRYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011238673.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thyroid hormone receptor interactor 12 (Trip12), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238673.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAGAAAACG 
GGGCAGGTGC CTAAAAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCGAAA AAGCCCAGAG CATTTCAGCA TATTGAGTCT 
TTCTCAGAAA CCAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATT GCCGTCAACA AGCAAGGCTC ACACCAGAAA GAGTGTGGCT 
GCTGGTAGTT CACGGAATCA GAAAAGAAAG AGGACAGAGA GTTCTTGTGT TAAAAGTGGT 
TCTGGGTCCG AATCAACTGG TGCCGAAGAG AGATCTGCAA AACCCATCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGATTCTTC TTCTGCTGCC 
TCTACTTCCT CATCATCTTC AGCTATAGCC TCGGCCTCTT CTACTGTACC AGCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGTTCAGC ATCCAGCCCT 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGCTTCAG CCCCAAAGTT AGCCTACCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AGTCCACCAA GAAACGCAGT GAGTCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGAGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AGAGAGGAGC AGCTGAGGCC CGGCGGCAAG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGACAGTAAA TTCCTCAGCT GCCCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCG 
GATGATTCTG AGATGGGACG ATTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCTCCTCAC 
CTATTTGGTC CTCTCGGGCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTTCAA GGACTACAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCCG TCATTGAAAT GTGTCAGTTA TTGGTCATGG GGAATGAGGA GACACTAGGA 
GGGTTTCCTG TGAAGAGTGT TGTTCCAGCT TTGATAACAT TACTGCAGAT GGAACATAAT 
TTTGACATTA TGAATCATGC TTGCCGAGCC TTAACATATA TGATGGAAGC TCTCCCCCGG 
TCATCTGCTG TTGTAGTGGA TGCTATCCCT GTTTTCTTAG AAAAGTTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GCGACACAGT 
AAAGCCATTT TACAAGCGGG TGGTTTGGCA GATTGCTTGT TGTATCTAGA ATTCTTCAGC 
ATCAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACACCA 
GATGAGTTTC ATTTTGTGGC AGATTCCCTA CCATTGCTTA CCCAAAGGCT AACTCATCAG 
GACAAAAAGT CAGTAGAAAG TACTTGCCTT TGTTTTGCAC GTTTAGTGGA CAACTTTCAA 
CATGAGGAGA ACTTACTGCA ACAGGTTGCT TCCAAAGATC TACTTACAAA TGTTCAACAA 
TTGTTGGTAG TGACTCCACC GATTTTAAGT TCTGGGATGT TTATTATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACA TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACAC TTCACTTCCT GCTATGTGGT GCCTCCAATG GAAGTTGTCA AGAGCAGATT 
GACCTTGTTC CACGAAGTCC TCAAGAGCTG TATGAGCTGA CATCTTTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATCTTC GCAGTTGATA CCATGTTGAA AAAGGGAAAT 
GCACAGAACA CAGATGGTGC AATATGGCAG TGGCGGGATG ACCGAGGCCT CTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAAGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAATCCCTT AGCCAATTCT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT AATGAAAGAA 
GATCCGGAAC TGGCTAAGTC TTTCATTAAG ACCTTGTTTG GTGTTCTCTA TGAAGTGTAC 
AGCTCCTCAG CAGGCCCTGC GGTCAGACAC AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCTG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCAA TGCTATCCAG CCAAGACCTG AAGATAGTAG TTGGAGCTCT TCAGATGGCA 
GAAATCCTAA TGCAAAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGGAG AGAAGGTGTG 
ATGCATCAAG TCAAACATTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC CCCCAAGGCA 
TGTACAAATG GGTCAGGATC CCTGGGATCT ACAACACCAG CCAGCAGTGG GACTGCAACG 
GCAGCAACCA ACGCCTCTGC TGATCTGGGG TCACCTAGCT TGCAGCATAG CAGAGATGAT 
TCTTTGGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TCAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAATATTCC CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CCTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAATATCGA ACCAGCACGA 
ACTGCAGGAG TTAGTGGTCT TGCCAGGGCA GCCTCAAAAG ACACCATATC CAATAATAGA 
GAAAAAATTA AAGGCTGGAT TAAGGAGCAG GCACATAAAT TTGTTGAGCG TTATTTTAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCC TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCTTCATTT GAAATCCAAC ATAGTGGGTT TGTGAAACAA 
CTGTTGCTGT ATTTGACATC TAAGAATGAA AAGGATGCTG TCGGCAGAGA GATCCGGTTA 
AAACGTTTCC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC TGTTGGAAGA 
GTAGAACCAG TGGGCCATGC ACCTTTGCTG GCATTGGTTC ACAAGATGAA CAACTGTCTC 
AGCCAAATGG AGCAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGGAA CGGAGCTGGA 
GGAAGCTTTT CTCTCAACAG AGGATCACAA GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGTCAGT TACAAAGACA CCCAGACTGT GCAAATGTGA AGCAGTGGAA AGGCGGGCCT 
GTCAAGATTG ATCCCCTGGC TCTGGTGCAA GCTATTGAGA GATACCTTGT GGTTAGAGGT 
ATTTTCCGAT ATGGAAGAGT AAGAGAAGAT GATGAAGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAGTCTTT GGCTGCTCAA TTCTTAAATT CGGGAAATGT AAGGCACAGG 
CTACAGTTTT ATATTGGAGA ACATTTACTA CCATATAACA TGACCGTGTA TCAGGCAGTG 
CGGCAATTCA GTGTACAGGC TGAAGATGAA AGAGAATCTA CTGATGATGA GAGCAATCCT 
CTGGGAAGAG CTGGAATTTG GACAAAGACT CATACAATAT GGTACAAACC TGTGAGGGAG 
GATGAAGAAA GTACTAAAGA TTGTGTTGGT GGCAAAAGGG GGAGAGCCCA AACAGCTCCA 
ACAAAAACTT CCCCCAGAAA TGCAAAGAAG CATGATGAGT TATGGCATGA TGGAGTGTGC 
CCGTCAGTAG CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATCACC 
TTTGAAGACC CATCCTTAGA TGTAATACTT CTTTTAAGAG TTTTACATGC CATCAGTCGA 
TATTGGTATT ACTTGTATGA TAATGCGATG TGCAAGGAGA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AATTAACAGC AAAAGCAAAT AGGCAGCTTC AAGACCCTCT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTCACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GACAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG GGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGACAG CAGAGTTGCG 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTACTGAA ACAAGCTGAG 
TCTGTGATGC AGGACCTTGG CAGCTCTCGG GCCATGTTAG AAATCCAATA TGAAAATGAG 
GTTGGTACAG GTCTGGGGCC AACACTGGAG TTTTATGCAC TTGTTTCTCA GGAACTACAG 
AGAGCTGACT TGTGTCTCTG GAGGGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCACAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCTAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CTCTTGGCCT GCCCTTTTAT 
AAGTGGATGC TGCGGCAGGA AACTTCATTG ACATCTCATG ATTTATTTGA CATTGATCCT 
GTTGTAGCCA GATCAGTATA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTGGAG 
CAGGATAAAT CCCAGACCAA AGAGAGTCTA CAATATGCAT TGGAAACTCT TACGATGAAT 
GGCTGTTCAG TGGAAGATCT GGGATTGGAT TTTACGCTGC CTGGGTTTCC CAACATTGAA 
TTGAAGAAAG GAGGGAAGGA TATTCCAGTT ACTATCCACA ATTTGGAAGA GTACCTGAGA 
TTGGTGATAT TCTGGGCACT AAATGAAGGT GTTTGTCGAC AGTTTGACTC ATTCAGAGAT 
GGATTTGAAT CGGTCTTCCC GCTCTGCCAT CTTCAGTACT TCTACCCAGA AGAGCTGGAC 
CAGCTCCTGT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACATTGAT GGAATGCTGC 
AGGCCTGATC ATGGCTATAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTG TTTCTCCAGT TTGTGACTGG TAGCCCACGA 
TTGCCAGTTG GAGGATTTCG GAGTTTAAAT CCACCTCTGA CAATTGTGCG GAAGACATTT 
GAATCAACAG AAAACCCAGA TGACTTCTTA CCATCAGTAA TGACTTGTGT GAACTATCTT 
AAATTGCCGG ACTACTCGAG CATTGACATA ATGCGGGACA AACTCTTGAT AGCAGCAAGA 
GAAGGCCAAC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential effects on ARF stability by normal versus oncogenic levels of c-Myc expression.
Molecular cell51(1)46-56(2013 Jul)
Chen D,Kon N,Zhong J,Zhang P,Yu L,Gu W

TRADD contributes to tumour suppression by regulating ULF-dependent p19Arf ubiquitylation.
Nature cell biology14(6)625-33(2012 May)
Chio II,Sasaki M,Ghazarian D,Moreno J,Done S,Ueda T,Inoue S,Chang YL,Chen NJ,Mak TW

The E3 ubiquitin ligase activity of Trip12 is essential for mouse embryogenesis.
PloS one6(10)e25871(2011)
Kajiro M,Tsuchiya M,Kawabe Y,Furumai R,Iwasaki N,Hayashi Y,Katano M,Nakajima Y,Goto N,Watanabe T,Murayama A,Oishi H,Ema M,Takahashi S,Kishimoto H,Yanagisawa J

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Genes & development20(19)2713-27(2006 Oct)
Evsikov AV,Graber JH,Brockman JM,Hampl A,Holbrook AE,Singh P,Eppig JJ,Solter D,Knowles BB

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

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Trip12 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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