Gria1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gria1
Entrez Gene ID	14799
Full Name	glutamate receptor, ionotropic, AMPA1 (alpha 1)
Synonyms	2900051M01Rik,Glr-1,Glr1,GluA1,GluR-A,GluRA,Glur-1,Glur1,HIPA1,gluR-K1
General protein information	Preferred Names glutamate receptor, ionotropic, AMPA1 (alpha 1) Names glutamate receptor 1 AMPA-selective glutamate receptor 1 glutamate receptor A glutamate receptor ionotropic, AMPA 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B1.3\|11 34.51 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532234.3	XP_006532297.1	glutamate receptor 1 isoform X1
XM_017314275.1	XP_017169764.1	glutamate receptor 1 isoform X2
XM_017314275.2	XP_017169764.1	glutamate receptor 1 isoform X2
NM_001113325.2	NP_001106796.1	glutamate receptor 1 isoform 1 precursor
NM_001252403.1	NP_001239332.1	glutamate receptor 1 isoform 3
NM_008165.4	NP_032191.2	glutamate receptor 1 isoform 2 precursor
XM_006532234.4	XP_006532297.1	glutamate receptor 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GRIA1	NP_001107655.1
Canis lupus familiaris (dog)	GRIA1	XP_546286.2
Bos taurus (cattle)	GRIA1	XP_002689365.1
Danio rerio (zebrafish)	gria1b	NP_991293.1
Rattus norvegicus (Norway rat)	Gria1	NP_113796.1
	GRIA1	XP_002804636.1
Mus musculus (house mouse)	Gria1	NP_001106796.1
Gallus gallus (chicken)	GRIA1	NP_001001774.1
Danio rerio (zebrafish)	gria1a	NP_991161.1
Pan troglodytes (chimpanzee)	GRIA1	XP_003310973.1
Xenopus tropicalis (tropical clawed frog)	gria1	XP_002941536.1

Gene Ontology

The following Gria1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gria1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23174	NM_008165.4 Latest version!	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu23170	NM_001252403.1 Latest version!	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu23143	NM_001113325.2 Latest version!	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
OMu23170	XM_006532234.4 Latest version!	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu77744	XM_017314275.2 Latest version!	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu77744	XM_017314275.1	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OMu23170	XM_006532234.3	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gria1 ( NM_008165.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032191.2 Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant 2, mRNA.

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CloneID	OMu23174
Clone ID Related Accession (Same CDS sequence)	NM_008165.4
Accession Version	NM_008165.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	glutamate receptor 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL663055.13 and BC056397.1. On Nov 18, 2011 this sequence version replaced NM_008165.3. Transcript Variant: This variant (2) uses an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 2) with a distinct internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056397.1, BC060702.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGTACA TCTTTGCCTT TTTCTGCACC GGTTTTCTAG GTGCGGTTGT GGGTGCCAAT 
TTCCCCAACA ATATCCAGAT AGGGGGATTA TTTCCAAACC AACAATCACA GGAACATGCG 
GCTTTTAGGT TTGCTTTGTC ACAACTCACG GAGCCCCCCA AGCTGCTTCC CCAGATCGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACTTACCGAT TCTGTTCCCA GTTCTCCAAA 
GGAGTGTACG CCATCTTTGG ATTTTATGAA CGAAGGACTG TCAACATGCT GACCTCCTTC 
TGTGGGGCCC TCCATGTGTG CTTCATCACT CCAAGTTTTC CCGTTGACAC ATCCAATCAG 
TTTGTCCTTC AGCTGCGCCC GGAACTACAG GAAGCTCTCA TTAGCATTAT CGACCATTAC 
AAGTGGCAGA CTTTTGTCTA CATTTATGAT GCTGACCGGG GCCTGTCAGT CCTGCAGAGA 
GTCTTGGATA CAGCCGCCGA GAAGAACTGG CAGGTGACGG CTGTCAACAT TCTAACAACC 
ACGGAGGAAG GATACCGGAT GCTCTTTCAG GACCTGGAGA AGAAAAAGGA GAGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAC GCCATCCTGG GCCAGATTGT GAAGCTAGAA 
AAGAACGGCA TCGGGTACCA CTACATCCTC GCCAACCTGG GCTTCATGGA CATTGACTTA 
AATAAGTTCA AGGAGAGTGG AGCCAATGTG ACAGGTTTCC AACTGGTGAA CTACACAGAC 
ACGATCCCAG CCAGAATCAT GCAGCAGTGG AGGACAAGTG ACGCTCGGGA CCACACCAGG 
GTGGACTGGA AGAGGCCAAA GTACACTTCT GCTCTTACCT ATGATGGTGT GAAGGTGATG 
GCGGAGGCCT TCCAGAGCCT GCGGAGGCAG AGGATTGACA TATCCCGGCG AGGGAATGCT 
GGGGACTGTC TGGCTAACCC AGCTGTGCCC TGGGGCCAAG GGATCGACAT CCAGAGAGCC 
CTGCAGCAGG TGCGCTTTGA AGGTTTGACA GGAAATGTGC AGTTTAACGA GAAAGGGCGC 
CGGACCAACT ACACCCTCCA TGTGATCGAA ATGAAGCATG ATGGAATCCG CAAGATTGGT 
TACTGGAATG AAGATGATAA ATTTGTCCCC GCAGCCACGG ACGCTCAGGC TGGAGGGGAC 
AACTCAAGCG TCCAGAATAG AACCTACATC GTCACGACTA TCCTCGAAGA TCCTTACGTG 
ATGCTTAAAA AGAATGCCAA CCAATTTGAA GGCAATGACC GCTATGAGGG CTACTGCGTG 
GAACTGGCTG CGGAGATCGC CAAGCACGTG GGCTATTCCT ACCGACTTGA GATTGTCAGC 
GACGGCAAAT ACGGAGCCCG GGATCCTGAC ACAAAGGCCT GGAATGGCAT GGTGGGAGAG 
CTAGTCTATG GAAGAGCAGA TGTGGCGGTG GCCCCCTTGA CCATAACCTT GGTCCGGGAG 
GAAGTCATCG ACTTCTCCAA GCCATTCATG AGTTTGGGAA TCTCCATTAT GATTAAGAAG 
CCACAGAAGT CCAAGCCAGG TGTCTTCTCC TTTCTTGACC CTTTGGCCTA CGAGATCTGG 
ATGTGTATAG TGTTTGCCTA CATTGGAGTG AGCGTCGTCC TCTTCCTGGT CAGCCGTTTC 
AGTCCTTATG AATGGCACAG TGAAGAGTTT GAAGAAGGAC GAGATCAGAC AACCAGTGAC 
CAGTCAAATG AGTTTGGCAT ATTCAACAGC CTGTGGTTCT CGCTGGGGGC CTTCATGCAG 
CAAGGATGTG ACATTTCCCC CAGGTCCCTG TCTGGACGCA TCGTCGGCGG TGTCTGGTGG 
TTCTTCACTT TGATTATCAT CTCCTCATAC ACAGCCAACC TGGCTGCCTT CCTGACTGTG 
GAAAGGATGG TGTCTCCCAT CGAGAGTGCA GAGGACCTGG CAAAGCAGAC GGAAATTGCT 
TATGGGACAT TGGAAGCAGG ATCCACTAAG GAGTTCTTCA GGAGGTCTAA AATCGCTGTG 
TTTGAGAAGA TGTGGACATA CATGAAGTCT GCAGAACCGT CTGTGTTTGT TCGGACCACA 
GAGGAGGGCA TGATCAGAGT GAGAAAGTCT AAAGGCAAAT ATGCCTACCT CCTGGAGTCC 
ACCATGAATG AGTACATTGA GCAACGCAAG CCCTGTGACA CCATGAAAGT GGGAGGTAAC 
TTGGATTCCA AAGGCTATGG CATTGCAACA CCCAAGGGGT CCGCCCTGAG AAATCCAGTA 
AACCTGGCAG TGTTAAAACT GAACGAGCAG GGGCTTTTGG ACAAATTGAA AAACAAATGG 
TGGTACGACA AGGGCGAGTG CGGCAGCGGG GGAGGTGACT CCAAGGACAA GACCAGTGCT 
CTGAGCCTGA GCAATGTGGC AGGCGTGTTC TACATCCTGA TTGGAGGGCT GGGATTGGCC 
ATGCTGGTTG CCTTAATCGA GTTCTGCTAC AAATCCCGTA GCGAGTCGAA GCGGATGAAG 
GGTTTCTGTT TGATTCCACA GCAATCCATC AATGAAGCCA TACGGACATC GACCCTCCCC 
AGGAACAGCG GGGCAGGAGC CAGCGGAGGA AGTGGCAGTG GAGAGAATGG CAGAGTGGTC 
AGCCAGGACT TCCCCAAGTC CATGCAATCC ATTCCCTGCA TGAGCCACAG TTCAGGGATG 
CCCTTGGGAG CCACAGGATT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032191.2
CDS	417..3140
Translation	MPYIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGSGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	NM_008165.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008165.4

ORF Insert Sequence:

ATGCCGTACA TCTTTGCCTT TTTCTGCACC GGTTTTCTAG GTGCGGTTGT GGGTGCCAAT 
TTCCCCAACA ATATCCAGAT AGGGGGATTA TTTCCAAACC AACAATCACA GGAACATGCG 
GCTTTTAGGT TTGCTTTGTC ACAACTCACG GAGCCCCCCA AGCTGCTTCC CCAGATCGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACTTACCGAT TCTGTTCCCA GTTCTCCAAA 
GGAGTGTACG CCATCTTTGG ATTTTATGAA CGAAGGACTG TCAACATGCT GACCTCCTTC 
TGTGGGGCCC TCCATGTGTG CTTCATCACT CCAAGTTTTC CCGTTGACAC ATCCAATCAG 
TTTGTCCTTC AGCTGCGCCC GGAACTACAG GAAGCTCTCA TTAGCATTAT CGACCATTAC 
AAGTGGCAGA CTTTTGTCTA CATTTATGAT GCTGACCGGG GCCTGTCAGT CCTGCAGAGA 
GTCTTGGATA CAGCCGCCGA GAAGAACTGG CAGGTGACGG CTGTCAACAT TCTAACAACC 
ACGGAGGAAG GATACCGGAT GCTCTTTCAG GACCTGGAGA AGAAAAAGGA GAGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAC GCCATCCTGG GCCAGATTGT GAAGCTAGAA 
AAGAACGGCA TCGGGTACCA CTACATCCTC GCCAACCTGG GCTTCATGGA CATTGACTTA 
AATAAGTTCA AGGAGAGTGG AGCCAATGTG ACAGGTTTCC AACTGGTGAA CTACACAGAC 
ACGATCCCAG CCAGAATCAT GCAGCAGTGG AGGACAAGTG ACGCTCGGGA CCACACCAGG 
GTGGACTGGA AGAGGCCAAA GTACACTTCT GCTCTTACCT ATGATGGTGT GAAGGTGATG 
GCGGAGGCCT TCCAGAGCCT GCGGAGGCAG AGGATTGACA TATCCCGGCG AGGGAATGCT 
GGGGACTGTC TGGCTAACCC AGCTGTGCCC TGGGGCCAAG GGATCGACAT CCAGAGAGCC 
CTGCAGCAGG TGCGCTTTGA AGGTTTGACA GGAAATGTGC AGTTTAACGA GAAAGGGCGC 
CGGACCAACT ACACCCTCCA TGTGATCGAA ATGAAGCATG ATGGAATCCG CAAGATTGGT 
TACTGGAATG AAGATGATAA ATTTGTCCCC GCAGCCACGG ACGCTCAGGC TGGAGGGGAC 
AACTCAAGCG TCCAGAATAG AACCTACATC GTCACGACTA TCCTCGAAGA TCCTTACGTG 
ATGCTTAAAA AGAATGCCAA CCAATTTGAA GGCAATGACC GCTATGAGGG CTACTGCGTG 
GAACTGGCTG CGGAGATCGC CAAGCACGTG GGCTATTCCT ACCGACTTGA GATTGTCAGC 
GACGGCAAAT ACGGAGCCCG GGATCCTGAC ACAAAGGCCT GGAATGGCAT GGTGGGAGAG 
CTAGTCTATG GAAGAGCAGA TGTGGCGGTG GCCCCCTTGA CCATAACCTT GGTCCGGGAG 
GAAGTCATCG ACTTCTCCAA GCCATTCATG AGTTTGGGAA TCTCCATTAT GATTAAGAAG 
CCACAGAAGT CCAAGCCAGG TGTCTTCTCC TTTCTTGACC CTTTGGCCTA CGAGATCTGG 
ATGTGTATAG TGTTTGCCTA CATTGGAGTG AGCGTCGTCC TCTTCCTGGT CAGCCGTTTC 
AGTCCTTATG AATGGCACAG TGAAGAGTTT GAAGAAGGAC GAGATCAGAC AACCAGTGAC 
CAGTCAAATG AGTTTGGCAT ATTCAACAGC CTGTGGTTCT CGCTGGGGGC CTTCATGCAG 
CAAGGATGTG ACATTTCCCC CAGGTCCCTG TCTGGACGCA TCGTCGGCGG TGTCTGGTGG 
TTCTTCACTT TGATTATCAT CTCCTCATAC ACAGCCAACC TGGCTGCCTT CCTGACTGTG 
GAAAGGATGG TGTCTCCCAT CGAGAGTGCA GAGGACCTGG CAAAGCAGAC GGAAATTGCT 
TATGGGACAT TGGAAGCAGG ATCCACTAAG GAGTTCTTCA GGAGGTCTAA AATCGCTGTG 
TTTGAGAAGA TGTGGACATA CATGAAGTCT GCAGAACCGT CTGTGTTTGT TCGGACCACA 
GAGGAGGGCA TGATCAGAGT GAGAAAGTCT AAAGGCAAAT ATGCCTACCT CCTGGAGTCC 
ACCATGAATG AGTACATTGA GCAACGCAAG CCCTGTGACA CCATGAAAGT GGGAGGTAAC 
TTGGATTCCA AAGGCTATGG CATTGCAACA CCCAAGGGGT CCGCCCTGAG AAATCCAGTA 
AACCTGGCAG TGTTAAAACT GAACGAGCAG GGGCTTTTGG ACAAATTGAA AAACAAATGG 
TGGTACGACA AGGGCGAGTG CGGCAGCGGG GGAGGTGACT CCAAGGACAA GACCAGTGCT 
CTGAGCCTGA GCAATGTGGC AGGCGTGTTC TACATCCTGA TTGGAGGGCT GGGATTGGCC 
ATGCTGGTTG CCTTAATCGA GTTCTGCTAC AAATCCCGTA GCGAGTCGAA GCGGATGAAG 
GGTTTCTGTT TGATTCCACA GCAATCCATC AATGAAGCCA TACGGACATC GACCCTCCCC 
AGGAACAGCG GGGCAGGAGC CAGCGGAGGA AGTGGCAGTG GAGAGAATGG CAGAGTGGTC 
AGCCAGGACT TCCCCAAGTC CATGCAATCC ATTCCCTGCA TGAGCCACAG TTCAGGGATG 
CCCTTGGGAG CCACAGGATT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gria1 ( XM_017314275.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169764.1 Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu77744
Clone ID Related Accession (Same CDS sequence)	XM_017314275.1 , XM_017314275.2
Accession Version	XM_017314275.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glutamate receptor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTACC GATTCTGTTC CCAGTTCTCC AAAGGAGTGT ACGCCATCTT TGGATTTTAT 
GAACGAAGGA CTGTCAACAT GCTGACCTCC TTCTGTGGGG CCCTCCATGT GTGCTTCATC 
ACTCCAAGTT TTCCCGTTGA CACATCCAAT CAGTTTGTCC TTCAGCTGCG CCCGGAACTA 
CAGGAAGCTC TCATTAGCAT TATCGACCAT TACAAGTGGC AGACTTTTGT CTACATTTAT 
GATGCTGACC GGGGCCTGTC AGTCCTGCAG AGAGTCTTGG ATACAGCCGC CGAGAAGAAC 
TGGCAGGTGA CGGCTGTCAA CATTCTAACA ACCACGGAGG AAGGATACCG GATGCTCTTT 
CAGGACCTGG AGAAGAAAAA GGAGAGGCTG GTGGTGGTGG ACTGTGAATC AGAACGCCTC 
AACGCCATCC TGGGCCAGAT TGTGAAGCTA GAAAAGAACG GCATCGGGTA CCACTACATC 
CTCGCCAACC TGGGCTTCAT GGACATTGAC TTAAATAAGT TCAAGGAGAG TGGAGCCAAT 
GTGACAGGTT TCCAACTGGT GAACTACACA GACACGATCC CAGCCAGAAT CATGCAGCAG 
TGGAGGACAA GTGACGCTCG GGACCACACC AGGGTGGACT GGAAGAGGCC AAAGTACACT 
TCTGCTCTTA CCTATGATGG TGTGAAGGTG ATGGCGGAGG CCTTCCAGAG CCTGCGGAGG 
CAGAGGATTG ACATATCCCG GCGAGGGAAT GCTGGGGACT GTCTGGCTAA CCCAGCTGTG 
CCCTGGGGCC AAGGGATCGA CATCCAGAGA GCCCTGCAGC AGGTGCGCTT TGAAGGTTTG 
ACAGGAAATG TGCAGTTTAA CGAGAAAGGG CGCCGGACCA ACTACACCCT CCATGTGATC 
GAAATGAAGC ATGATGGAAT CCGCAAGATT GGTTACTGGA ATGAAGATGA TAAATTTGTC 
CCCGCAGCCA CGGACGCTCA GGCTGGAGGG GACAACTCAA GCGTCCAGAA TAGAACCTAC 
ATCGTCACGA CTATCCTCGA AGATCCTTAC GTGATGCTTA AAAAGAATGC CAACCAATTT 
GAAGGCAATG ACCGCTATGA GGGCTACTGC GTGGAACTGG CTGCGGAGAT CGCCAAGCAC 
GTGGGCTATT CCTACCGACT TGAGATTGTC AGCGACGGCA AATACGGAGC CCGGGATCCT 
GACACAAAGG CCTGGAATGG CATGGTGGGA GAGCTAGTCT ATGGAAGAGC AGATGTGGCG 
GTGGCCCCCT TGACCATAAC CTTGGTCCGG GAGGAAGTCA TCGACTTCTC CAAGCCATTC 
ATGAGTTTGG GAATCTCCAT TATGATTAAG AAGCCACAGA AGTCCAAGCC AGGTGTCTTC 
TCCTTTCTTG ACCCTTTGGC CTACGAGATC TGGATGTGTA TAGTGTTTGC CTACATTGGA 
GTGAGCGTCG TCCTCTTCCT GGTCAGCCGT TTCAGTCCTT ATGAATGGCA CAGTGAAGAG 
TTTGAAGAAG GACGAGATCA GACAACCAGT GACCAGTCAA ATGAGTTTGG CATATTCAAC 
AGCCTGTGGT TCTCGCTGGG GGCCTTCATG CAGCAAGGAT GTGACATTTC CCCCAGGTCC 
CTGTCTGGAC GCATCGTCGG CGGTGTCTGG TGGTTCTTCA CTTTGATTAT CATCTCCTCA 
TACACAGCCA ACCTGGCTGC CTTCCTGACT GTGGAAAGGA TGGTGTCTCC CATCGAGAGT 
GCAGAGGACC TGGCAAAGCA GACGGAAATT GCTTATGGGA CATTGGAAGC AGGATCCACT 
AAGGAGTTCT TCAGGAGGTC TAAAATCGCT GTGTTTGAGA AGATGTGGAC ATACATGAAG 
TCTGCAGAAC CGTCTGTGTT TGTTCGGACC ACAGAGGAGG GCATGATCAG AGTGAGAAAG 
TCTAAAGGCA AATATGCCTA CCTCCTGGAG TCCACCATGA ATGAGTACAT TGAGCAACGC 
AAGCCCTGTG ACACCATGAA AGTGGGAGGT AACTTGGATT CCAAAGGCTA TGGCATTGCA 
ACACCCAAGG GGTCCGCCCT GAGAAATCCA GTAAACCTGG CAGTGTTAAA ACTGAACGAG 
CAGGGGCTTT TGGACAAATT GAAAAACAAA TGGTGGTACG ACAAGGGCGA GTGCGGCAGC 
GGGGGAGGTG ACTCCAAGGA CAAGACCAGT GCTCTGAGCC TGAGCAATGT GGCAGGCGTG 
TTCTACATCC TGATTGGAGG GCTGGGATTG GCCATGCTGG TTGCCTTAAT CGAGTTCTGC 
TACAAATCCC GTAGCGAGTC GAAGCGGATG AAGGGTTTCT GTTTGATTCC ACAGCAATCC 
ATCAATGAAG CCATACGGAC ATCGACCCTC CCCAGGAACA GCGGGGCAGG AGCCAGCGGA 
GGAAGTGGCA GTGGAGAGAA TGGCAGAGTG GTCAGCCAGG ACTTCCCCAA GTCCATGCAA 
TCCATTCCCT GCATGAGCCA CAGTTCAGGG ATGCCCTTGG GAGCCACAGG ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169764.1
CDS	399..2915
Translation	MTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGSGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	XM_017314275.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314275.1

ORF Insert Sequence:

ATGACTTACC GATTCTGTTC CCAGTTCTCC AAAGGAGTGT ACGCCATCTT TGGATTTTAT 
GAACGAAGGA CTGTCAACAT GCTGACCTCC TTCTGTGGGG CCCTCCATGT GTGCTTCATC 
ACTCCAAGTT TTCCCGTTGA CACATCCAAT CAGTTTGTCC TTCAGCTGCG CCCGGAACTA 
CAGGAAGCTC TCATTAGCAT TATCGACCAT TACAAGTGGC AGACTTTTGT CTACATTTAT 
GATGCTGACC GGGGCCTGTC AGTCCTGCAG AGAGTCTTGG ATACAGCCGC CGAGAAGAAC 
TGGCAGGTGA CGGCTGTCAA CATTCTAACA ACCACGGAGG AAGGATACCG GATGCTCTTT 
CAGGACCTGG AGAAGAAAAA GGAGAGGCTG GTGGTGGTGG ACTGTGAATC AGAACGCCTC 
AACGCCATCC TGGGCCAGAT TGTGAAGCTA GAAAAGAACG GCATCGGGTA CCACTACATC 
CTCGCCAACC TGGGCTTCAT GGACATTGAC TTAAATAAGT TCAAGGAGAG TGGAGCCAAT 
GTGACAGGTT TCCAACTGGT GAACTACACA GACACGATCC CAGCCAGAAT CATGCAGCAG 
TGGAGGACAA GTGACGCTCG GGACCACACC AGGGTGGACT GGAAGAGGCC AAAGTACACT 
TCTGCTCTTA CCTATGATGG TGTGAAGGTG ATGGCGGAGG CCTTCCAGAG CCTGCGGAGG 
CAGAGGATTG ACATATCCCG GCGAGGGAAT GCTGGGGACT GTCTGGCTAA CCCAGCTGTG 
CCCTGGGGCC AAGGGATCGA CATCCAGAGA GCCCTGCAGC AGGTGCGCTT TGAAGGTTTG 
ACAGGAAATG TGCAGTTTAA CGAGAAAGGG CGCCGGACCA ACTACACCCT CCATGTGATC 
GAAATGAAGC ATGATGGAAT CCGCAAGATT GGTTACTGGA ATGAAGATGA TAAATTTGTC 
CCCGCAGCCA CGGACGCTCA GGCTGGAGGG GACAACTCAA GCGTCCAGAA TAGAACCTAC 
ATCGTCACGA CTATCCTCGA AGATCCTTAC GTGATGCTTA AAAAGAATGC CAACCAATTT 
GAAGGCAATG ACCGCTATGA GGGCTACTGC GTGGAACTGG CTGCGGAGAT CGCCAAGCAC 
GTGGGCTATT CCTACCGACT TGAGATTGTC AGCGACGGCA AATACGGAGC CCGGGATCCT 
GACACAAAGG CCTGGAATGG CATGGTGGGA GAGCTAGTCT ATGGAAGAGC AGATGTGGCG 
GTGGCCCCCT TGACCATAAC CTTGGTCCGG GAGGAAGTCA TCGACTTCTC CAAGCCATTC 
ATGAGTTTGG GAATCTCCAT TATGATTAAG AAGCCACAGA AGTCCAAGCC AGGTGTCTTC 
TCCTTTCTTG ACCCTTTGGC CTACGAGATC TGGATGTGTA TAGTGTTTGC CTACATTGGA 
GTGAGCGTCG TCCTCTTCCT GGTCAGCCGT TTCAGTCCTT ATGAATGGCA CAGTGAAGAG 
TTTGAAGAAG GACGAGATCA GACAACCAGT GACCAGTCAA ATGAGTTTGG CATATTCAAC 
AGCCTGTGGT TCTCGCTGGG GGCCTTCATG CAGCAAGGAT GTGACATTTC CCCCAGGTCC 
CTGTCTGGAC GCATCGTCGG CGGTGTCTGG TGGTTCTTCA CTTTGATTAT CATCTCCTCA 
TACACAGCCA ACCTGGCTGC CTTCCTGACT GTGGAAAGGA TGGTGTCTCC CATCGAGAGT 
GCAGAGGACC TGGCAAAGCA GACGGAAATT GCTTATGGGA CATTGGAAGC AGGATCCACT 
AAGGAGTTCT TCAGGAGGTC TAAAATCGCT GTGTTTGAGA AGATGTGGAC ATACATGAAG 
TCTGCAGAAC CGTCTGTGTT TGTTCGGACC ACAGAGGAGG GCATGATCAG AGTGAGAAAG 
TCTAAAGGCA AATATGCCTA CCTCCTGGAG TCCACCATGA ATGAGTACAT TGAGCAACGC 
AAGCCCTGTG ACACCATGAA AGTGGGAGGT AACTTGGATT CCAAAGGCTA TGGCATTGCA 
ACACCCAAGG GGTCCGCCCT GAGAAATCCA GTAAACCTGG CAGTGTTAAA ACTGAACGAG 
CAGGGGCTTT TGGACAAATT GAAAAACAAA TGGTGGTACG ACAAGGGCGA GTGCGGCAGC 
GGGGGAGGTG ACTCCAAGGA CAAGACCAGT GCTCTGAGCC TGAGCAATGT GGCAGGCGTG 
TTCTACATCC TGATTGGAGG GCTGGGATTG GCCATGCTGG TTGCCTTAAT CGAGTTCTGC 
TACAAATCCC GTAGCGAGTC GAAGCGGATG AAGGGTTTCT GTTTGATTCC ACAGCAATCC 
ATCAATGAAG CCATACGGAC ATCGACCCTC CCCAGGAACA GCGGGGCAGG AGCCAGCGGA 
GGAAGTGGCA GTGGAGAGAA TGGCAGAGTG GTCAGCCAGG ACTTCCCCAA GTCCATGCAA 
TCCATTCCCT GCATGAGCCA CAGTTCAGGG ATGCCCTTGG GAGCCACAGG ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gria1 ( NM_001252403.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239332.1 Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant 3, mRNA.

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CloneID	OMu23170
Clone ID Related Accession (Same CDS sequence)	XM_006532234.3 , NM_001252403.1 , XM_006532234.4
Accession Version	NM_001252403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	glutamate receptor 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK135107.1, AL672177.9 and BC056397.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK135107.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164141 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTACC GATTCTGTTC CCAGTTCTCC AAAGGAGTGT ACGCCATCTT TGGATTTTAT 
GAACGAAGGA CTGTCAACAT GCTGACCTCC TTCTGTGGGG CCCTCCATGT GTGCTTCATC 
ACTCCAAGTT TTCCCGTTGA CACATCCAAT CAGTTTGTCC TTCAGCTGCG CCCGGAACTA 
CAGGAAGCTC TCATTAGCAT TATCGACCAT TACAAGTGGC AGACTTTTGT CTACATTTAT 
GATGCTGACC GGGGCCTGTC AGTCCTGCAG AGAGTCTTGG ATACAGCCGC CGAGAAGAAC 
TGGCAGGTGA CGGCTGTCAA CATTCTAACA ACCACGGAGG AAGGATACCG GATGCTCTTT 
CAGGACCTGG AGAAGAAAAA GGAGAGGCTG GTGGTGGTGG ACTGTGAATC AGAACGCCTC 
AACGCCATCC TGGGCCAGAT TGTGAAGCTA GAAAAGAACG GCATCGGGTA CCACTACATC 
CTCGCCAACC TGGGCTTCAT GGACATTGAC TTAAATAAGT TCAAGGAGAG TGGAGCCAAT 
GTGACAGGTT TCCAACTGGT GAACTACACA GACACGATCC CAGCCAGAAT CATGCAGCAG 
TGGAGGACAA GTGACGCTCG GGACCACACC AGGGTGGACT GGAAGAGGCC AAAGTACACT 
TCTGCTCTTA CCTATGATGG TGTGAAGGTG ATGGCGGAGG CCTTCCAGAG CCTGCGGAGG 
CAGAGGATTG ACATATCCCG GCGAGGGAAT GCTGGGGACT GTCTGGCTAA CCCAGCTGTG 
CCCTGGGGCC AAGGGATCGA CATCCAGAGA GCCCTGCAGC AGGTGCGCTT TGAAGGTTTG 
ACAGGAAATG TGCAGTTTAA CGAGAAAGGG CGCCGGACCA ACTACACCCT CCATGTGATC 
GAAATGAAGC ATGATGGAAT CCGCAAGATT GGTTACTGGA ATGAAGATGA TAAATTTGTC 
CCCGCAGCCA CGGACGCTCA GGCTGGAGGG GACAACTCAA GCGTCCAGAA TAGAACCTAC 
ATCGTCACGA CTATCCTCGA AGATCCTTAC GTGATGCTTA AAAAGAATGC CAACCAATTT 
GAAGGCAATG ACCGCTATGA GGGCTACTGC GTGGAACTGG CTGCGGAGAT CGCCAAGCAC 
GTGGGCTATT CCTACCGACT TGAGATTGTC AGCGACGGCA AATACGGAGC CCGGGATCCT 
GACACAAAGG CCTGGAATGG CATGGTGGGA GAGCTAGTCT ATGGAAGAGC AGATGTGGCG 
GTGGCCCCCT TGACCATAAC CTTGGTCCGG GAGGAAGTCA TCGACTTCTC CAAGCCATTC 
ATGAGTTTGG GAATCTCCAT TATGATTAAG AAGCCACAGA AGTCCAAGCC AGGTGTCTTC 
TCCTTTCTTG ACCCTTTGGC CTACGAGATC TGGATGTGTA TAGTGTTTGC CTACATTGGA 
GTGAGCGTCG TCCTCTTCCT GGTCAGCCGT TTCAGTCCTT ATGAATGGCA CAGTGAAGAG 
TTTGAAGAAG GACGAGATCA GACAACCAGT GACCAGTCAA ATGAGTTTGG CATATTCAAC 
AGCCTGTGGT TCTCGCTGGG GGCCTTCATG CAGCAAGGAT GTGACATTTC CCCCAGGTCC 
CTGTCTGGAC GCATCGTCGG CGGTGTCTGG TGGTTCTTCA CTTTGATTAT CATCTCCTCA 
TACACAGCCA ACCTGGCTGC CTTCCTGACT GTGGAAAGGA TGGTGTCTCC CATCGAGAGT 
GCAGAGGACC TGGCAAAGCA GACGGAAATT GCTTATGGGA CATTGGAAGC AGGATCCACT 
AAGGAGTTCT TCAGGAGGTC TAAAATCGCT GTGTTTGAGA AGATGTGGAC ATACATGAAG 
TCTGCAGAAC CGTCTGTGTT TGTTCGGACC ACAGAGGAGG GCATGATCAG AGTGAGAAAG 
TCTAAAGGCA AATATGCCTA CCTCCTGGAG TCCACCATGA ATGAGTACAT TGAGCAACGC 
AAGCCCTGTG ACACCATGAA AGTGGGAGGT AACTTGGATT CCAAAGGCTA TGGCATTGCA 
ACACCCAAGG GGTCCGCCCT GAGAGGTCCC GTAAACCTAG CGGTTTTGAA ACTCAGTGAG 
CAAGGCGTCT TAGACAAGCT GAAAAGCAAA TGGTGGTACG ATAAAGGGGA ATGTGGAAGC 
AAGGACTCCG GAAGTAAGGA CAAGACCAGT GCTCTGAGCC TGAGCAATGT GGCAGGCGTG 
TTCTACATCC TGATTGGAGG GCTGGGATTG GCCATGCTGG TTGCCTTAAT CGAGTTCTGC 
TACAAATCCC GTAGCGAGTC GAAGCGGATG AAGGGTTTCT GTTTGATTCC ACAGCAATCC 
ATCAATGAAG CCATACGGAC ATCGACCCTC CCCAGGAACA GCGGGGCAGG AGCCAGCGGA 
GGAAGTGGCA GTGGAGAGAA TGGCAGAGTG GTCAGCCAGG ACTTCCCCAA GTCCATGCAA 
TCCATTCCCT GCATGAGCCA CAGTTCAGGG ATGCCCTTGG GAGCCACAGG ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239332.1
CDS	251..2767
Translation	MTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPVNLAVLKLSEQGVLDKLKSKWWYDKGECGSKDSGSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGSGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	NM_001252403.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252403.1

ORF Insert Sequence:

ATGACTTACC GATTCTGTTC CCAGTTCTCC AAAGGAGTGT ACGCCATCTT TGGATTTTAT 
GAACGAAGGA CTGTCAACAT GCTGACCTCC TTCTGTGGGG CCCTCCATGT GTGCTTCATC 
ACTCCAAGTT TTCCCGTTGA CACATCCAAT CAGTTTGTCC TTCAGCTGCG CCCGGAACTA 
CAGGAAGCTC TCATTAGCAT TATCGACCAT TACAAGTGGC AGACTTTTGT CTACATTTAT 
GATGCTGACC GGGGCCTGTC AGTCCTGCAG AGAGTCTTGG ATACAGCCGC CGAGAAGAAC 
TGGCAGGTGA CGGCTGTCAA CATTCTAACA ACCACGGAGG AAGGATACCG GATGCTCTTT 
CAGGACCTGG AGAAGAAAAA GGAGAGGCTG GTGGTGGTGG ACTGTGAATC AGAACGCCTC 
AACGCCATCC TGGGCCAGAT TGTGAAGCTA GAAAAGAACG GCATCGGGTA CCACTACATC 
CTCGCCAACC TGGGCTTCAT GGACATTGAC TTAAATAAGT TCAAGGAGAG TGGAGCCAAT 
GTGACAGGTT TCCAACTGGT GAACTACACA GACACGATCC CAGCCAGAAT CATGCAGCAG 
TGGAGGACAA GTGACGCTCG GGACCACACC AGGGTGGACT GGAAGAGGCC AAAGTACACT 
TCTGCTCTTA CCTATGATGG TGTGAAGGTG ATGGCGGAGG CCTTCCAGAG CCTGCGGAGG 
CAGAGGATTG ACATATCCCG GCGAGGGAAT GCTGGGGACT GTCTGGCTAA CCCAGCTGTG 
CCCTGGGGCC AAGGGATCGA CATCCAGAGA GCCCTGCAGC AGGTGCGCTT TGAAGGTTTG 
ACAGGAAATG TGCAGTTTAA CGAGAAAGGG CGCCGGACCA ACTACACCCT CCATGTGATC 
GAAATGAAGC ATGATGGAAT CCGCAAGATT GGTTACTGGA ATGAAGATGA TAAATTTGTC 
CCCGCAGCCA CGGACGCTCA GGCTGGAGGG GACAACTCAA GCGTCCAGAA TAGAACCTAC 
ATCGTCACGA CTATCCTCGA AGATCCTTAC GTGATGCTTA AAAAGAATGC CAACCAATTT 
GAAGGCAATG ACCGCTATGA GGGCTACTGC GTGGAACTGG CTGCGGAGAT CGCCAAGCAC 
GTGGGCTATT CCTACCGACT TGAGATTGTC AGCGACGGCA AATACGGAGC CCGGGATCCT 
GACACAAAGG CCTGGAATGG CATGGTGGGA GAGCTAGTCT ATGGAAGAGC AGATGTGGCG 
GTGGCCCCCT TGACCATAAC CTTGGTCCGG GAGGAAGTCA TCGACTTCTC CAAGCCATTC 
ATGAGTTTGG GAATCTCCAT TATGATTAAG AAGCCACAGA AGTCCAAGCC AGGTGTCTTC 
TCCTTTCTTG ACCCTTTGGC CTACGAGATC TGGATGTGTA TAGTGTTTGC CTACATTGGA 
GTGAGCGTCG TCCTCTTCCT GGTCAGCCGT TTCAGTCCTT ATGAATGGCA CAGTGAAGAG 
TTTGAAGAAG GACGAGATCA GACAACCAGT GACCAGTCAA ATGAGTTTGG CATATTCAAC 
AGCCTGTGGT TCTCGCTGGG GGCCTTCATG CAGCAAGGAT GTGACATTTC CCCCAGGTCC 
CTGTCTGGAC GCATCGTCGG CGGTGTCTGG TGGTTCTTCA CTTTGATTAT CATCTCCTCA 
TACACAGCCA ACCTGGCTGC CTTCCTGACT GTGGAAAGGA TGGTGTCTCC CATCGAGAGT 
GCAGAGGACC TGGCAAAGCA GACGGAAATT GCTTATGGGA CATTGGAAGC AGGATCCACT 
AAGGAGTTCT TCAGGAGGTC TAAAATCGCT GTGTTTGAGA AGATGTGGAC ATACATGAAG 
TCTGCAGAAC CGTCTGTGTT TGTTCGGACC ACAGAGGAGG GCATGATCAG AGTGAGAAAG 
TCTAAAGGCA AATATGCCTA CCTCCTGGAG TCCACCATGA ATGAGTACAT TGAGCAACGC 
AAGCCCTGTG ACACCATGAA AGTGGGAGGT AACTTGGATT CCAAAGGCTA TGGCATTGCA 
ACACCCAAGG GGTCCGCCCT GAGAGGTCCC GTAAACCTAG CGGTTTTGAA ACTCAGTGAG 
CAAGGCGTCT TAGACAAGCT GAAAAGCAAA TGGTGGTACG ATAAAGGGGA ATGTGGAAGC 
AAGGACTCCG GAAGTAAGGA CAAGACCAGT GCTCTGAGCC TGAGCAATGT GGCAGGCGTG 
TTCTACATCC TGATTGGAGG GCTGGGATTG GCCATGCTGG TTGCCTTAAT CGAGTTCTGC 
TACAAATCCC GTAGCGAGTC GAAGCGGATG AAGGGTTTCT GTTTGATTCC ACAGCAATCC 
ATCAATGAAG CCATACGGAC ATCGACCCTC CCCAGGAACA GCGGGGCAGG AGCCAGCGGA 
GGAAGTGGCA GTGGAGAGAA TGGCAGAGTG GTCAGCCAGG ACTTCCCCAA GTCCATGCAA 
TCCATTCCCT GCATGAGCCA CAGTTCAGGG ATGCCCTTGG GAGCCACAGG ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gria1 ( XM_006532234.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532297.1 Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23170
Clone ID Related Accession (Same CDS sequence)	XM_006532234.3 , NM_001252403.1 , XM_006532234.4
Accession Version	XM_006532234.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glutamate receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532234.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTACC GATTCTGTTC CCAGTTCTCC AAAGGAGTGT ACGCCATCTT TGGATTTTAT 
GAACGAAGGA CTGTCAACAT GCTGACCTCC TTCTGTGGGG CCCTCCATGT GTGCTTCATC 
ACTCCAAGTT TTCCCGTTGA CACATCCAAT CAGTTTGTCC TTCAGCTGCG CCCGGAACTA 
CAGGAAGCTC TCATTAGCAT TATCGACCAT TACAAGTGGC AGACTTTTGT CTACATTTAT 
GATGCTGACC GGGGCCTGTC AGTCCTGCAG AGAGTCTTGG ATACAGCCGC CGAGAAGAAC 
TGGCAGGTGA CGGCTGTCAA CATTCTAACA ACCACGGAGG AAGGATACCG GATGCTCTTT 
CAGGACCTGG AGAAGAAAAA GGAGAGGCTG GTGGTGGTGG ACTGTGAATC AGAACGCCTC 
AACGCCATCC TGGGCCAGAT TGTGAAGCTA GAAAAGAACG GCATCGGGTA CCACTACATC 
CTCGCCAACC TGGGCTTCAT GGACATTGAC TTAAATAAGT TCAAGGAGAG TGGAGCCAAT 
GTGACAGGTT TCCAACTGGT GAACTACACA GACACGATCC CAGCCAGAAT CATGCAGCAG 
TGGAGGACAA GTGACGCTCG GGACCACACC AGGGTGGACT GGAAGAGGCC AAAGTACACT 
TCTGCTCTTA CCTATGATGG TGTGAAGGTG ATGGCGGAGG CCTTCCAGAG CCTGCGGAGG 
CAGAGGATTG ACATATCCCG GCGAGGGAAT GCTGGGGACT GTCTGGCTAA CCCAGCTGTG 
CCCTGGGGCC AAGGGATCGA CATCCAGAGA GCCCTGCAGC AGGTGCGCTT TGAAGGTTTG 
ACAGGAAATG TGCAGTTTAA CGAGAAAGGG CGCCGGACCA ACTACACCCT CCATGTGATC 
GAAATGAAGC ATGATGGAAT CCGCAAGATT GGTTACTGGA ATGAAGATGA TAAATTTGTC 
CCCGCAGCCA CGGACGCTCA GGCTGGAGGG GACAACTCAA GCGTCCAGAA TAGAACCTAC 
ATCGTCACGA CTATCCTCGA AGATCCTTAC GTGATGCTTA AAAAGAATGC CAACCAATTT 
GAAGGCAATG ACCGCTATGA GGGCTACTGC GTGGAACTGG CTGCGGAGAT CGCCAAGCAC 
GTGGGCTATT CCTACCGACT TGAGATTGTC AGCGACGGCA AATACGGAGC CCGGGATCCT 
GACACAAAGG CCTGGAATGG CATGGTGGGA GAGCTAGTCT ATGGAAGAGC AGATGTGGCG 
GTGGCCCCCT TGACCATAAC CTTGGTCCGG GAGGAAGTCA TCGACTTCTC CAAGCCATTC 
ATGAGTTTGG GAATCTCCAT TATGATTAAG AAGCCACAGA AGTCCAAGCC AGGTGTCTTC 
TCCTTTCTTG ACCCTTTGGC CTACGAGATC TGGATGTGTA TAGTGTTTGC CTACATTGGA 
GTGAGCGTCG TCCTCTTCCT GGTCAGCCGT TTCAGTCCTT ATGAATGGCA CAGTGAAGAG 
TTTGAAGAAG GACGAGATCA GACAACCAGT GACCAGTCAA ATGAGTTTGG CATATTCAAC 
AGCCTGTGGT TCTCGCTGGG GGCCTTCATG CAGCAAGGAT GTGACATTTC CCCCAGGTCC 
CTGTCTGGAC GCATCGTCGG CGGTGTCTGG TGGTTCTTCA CTTTGATTAT CATCTCCTCA 
TACACAGCCA ACCTGGCTGC CTTCCTGACT GTGGAAAGGA TGGTGTCTCC CATCGAGAGT 
GCAGAGGACC TGGCAAAGCA GACGGAAATT GCTTATGGGA CATTGGAAGC AGGATCCACT 
AAGGAGTTCT TCAGGAGGTC TAAAATCGCT GTGTTTGAGA AGATGTGGAC ATACATGAAG 
TCTGCAGAAC CGTCTGTGTT TGTTCGGACC ACAGAGGAGG GCATGATCAG AGTGAGAAAG 
TCTAAAGGCA AATATGCCTA CCTCCTGGAG TCCACCATGA ATGAGTACAT TGAGCAACGC 
AAGCCCTGTG ACACCATGAA AGTGGGAGGT AACTTGGATT CCAAAGGCTA TGGCATTGCA 
ACACCCAAGG GGTCCGCCCT GAGAGGTCCC GTAAACCTAG CGGTTTTGAA ACTCAGTGAG 
CAAGGCGTCT TAGACAAGCT GAAAAGCAAA TGGTGGTACG ATAAAGGGGA ATGTGGAAGC 
AAGGACTCCG GAAGTAAGGA CAAGACCAGT GCTCTGAGCC TGAGCAATGT GGCAGGCGTG 
TTCTACATCC TGATTGGAGG GCTGGGATTG GCCATGCTGG TTGCCTTAAT CGAGTTCTGC 
TACAAATCCC GTAGCGAGTC GAAGCGGATG AAGGGTTTCT GTTTGATTCC ACAGCAATCC 
ATCAATGAAG CCATACGGAC ATCGACCCTC CCCAGGAACA GCGGGGCAGG AGCCAGCGGA 
GGAAGTGGCA GTGGAGAGAA TGGCAGAGTG GTCAGCCAGG ACTTCCCCAA GTCCATGCAA 
TCCATTCCCT GCATGAGCCA CAGTTCAGGG ATGCCCTTGG GAGCCACAGG ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532297.1
CDS	398..2914
Translation	MTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPVNLAVLKLSEQGVLDKLKSKWWYDKGECGSKDSGSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGSGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	XM_006532234.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532234.3

ORF Insert Sequence:

ATGACTTACC GATTCTGTTC CCAGTTCTCC AAAGGAGTGT ACGCCATCTT TGGATTTTAT 
GAACGAAGGA CTGTCAACAT GCTGACCTCC TTCTGTGGGG CCCTCCATGT GTGCTTCATC 
ACTCCAAGTT TTCCCGTTGA CACATCCAAT CAGTTTGTCC TTCAGCTGCG CCCGGAACTA 
CAGGAAGCTC TCATTAGCAT TATCGACCAT TACAAGTGGC AGACTTTTGT CTACATTTAT 
GATGCTGACC GGGGCCTGTC AGTCCTGCAG AGAGTCTTGG ATACAGCCGC CGAGAAGAAC 
TGGCAGGTGA CGGCTGTCAA CATTCTAACA ACCACGGAGG AAGGATACCG GATGCTCTTT 
CAGGACCTGG AGAAGAAAAA GGAGAGGCTG GTGGTGGTGG ACTGTGAATC AGAACGCCTC 
AACGCCATCC TGGGCCAGAT TGTGAAGCTA GAAAAGAACG GCATCGGGTA CCACTACATC 
CTCGCCAACC TGGGCTTCAT GGACATTGAC TTAAATAAGT TCAAGGAGAG TGGAGCCAAT 
GTGACAGGTT TCCAACTGGT GAACTACACA GACACGATCC CAGCCAGAAT CATGCAGCAG 
TGGAGGACAA GTGACGCTCG GGACCACACC AGGGTGGACT GGAAGAGGCC AAAGTACACT 
TCTGCTCTTA CCTATGATGG TGTGAAGGTG ATGGCGGAGG CCTTCCAGAG CCTGCGGAGG 
CAGAGGATTG ACATATCCCG GCGAGGGAAT GCTGGGGACT GTCTGGCTAA CCCAGCTGTG 
CCCTGGGGCC AAGGGATCGA CATCCAGAGA GCCCTGCAGC AGGTGCGCTT TGAAGGTTTG 
ACAGGAAATG TGCAGTTTAA CGAGAAAGGG CGCCGGACCA ACTACACCCT CCATGTGATC 
GAAATGAAGC ATGATGGAAT CCGCAAGATT GGTTACTGGA ATGAAGATGA TAAATTTGTC 
CCCGCAGCCA CGGACGCTCA GGCTGGAGGG GACAACTCAA GCGTCCAGAA TAGAACCTAC 
ATCGTCACGA CTATCCTCGA AGATCCTTAC GTGATGCTTA AAAAGAATGC CAACCAATTT 
GAAGGCAATG ACCGCTATGA GGGCTACTGC GTGGAACTGG CTGCGGAGAT CGCCAAGCAC 
GTGGGCTATT CCTACCGACT TGAGATTGTC AGCGACGGCA AATACGGAGC CCGGGATCCT 
GACACAAAGG CCTGGAATGG CATGGTGGGA GAGCTAGTCT ATGGAAGAGC AGATGTGGCG 
GTGGCCCCCT TGACCATAAC CTTGGTCCGG GAGGAAGTCA TCGACTTCTC CAAGCCATTC 
ATGAGTTTGG GAATCTCCAT TATGATTAAG AAGCCACAGA AGTCCAAGCC AGGTGTCTTC 
TCCTTTCTTG ACCCTTTGGC CTACGAGATC TGGATGTGTA TAGTGTTTGC CTACATTGGA 
GTGAGCGTCG TCCTCTTCCT GGTCAGCCGT TTCAGTCCTT ATGAATGGCA CAGTGAAGAG 
TTTGAAGAAG GACGAGATCA GACAACCAGT GACCAGTCAA ATGAGTTTGG CATATTCAAC 
AGCCTGTGGT TCTCGCTGGG GGCCTTCATG CAGCAAGGAT GTGACATTTC CCCCAGGTCC 
CTGTCTGGAC GCATCGTCGG CGGTGTCTGG TGGTTCTTCA CTTTGATTAT CATCTCCTCA 
TACACAGCCA ACCTGGCTGC CTTCCTGACT GTGGAAAGGA TGGTGTCTCC CATCGAGAGT 
GCAGAGGACC TGGCAAAGCA GACGGAAATT GCTTATGGGA CATTGGAAGC AGGATCCACT 
AAGGAGTTCT TCAGGAGGTC TAAAATCGCT GTGTTTGAGA AGATGTGGAC ATACATGAAG 
TCTGCAGAAC CGTCTGTGTT TGTTCGGACC ACAGAGGAGG GCATGATCAG AGTGAGAAAG 
TCTAAAGGCA AATATGCCTA CCTCCTGGAG TCCACCATGA ATGAGTACAT TGAGCAACGC 
AAGCCCTGTG ACACCATGAA AGTGGGAGGT AACTTGGATT CCAAAGGCTA TGGCATTGCA 
ACACCCAAGG GGTCCGCCCT GAGAGGTCCC GTAAACCTAG CGGTTTTGAA ACTCAGTGAG 
CAAGGCGTCT TAGACAAGCT GAAAAGCAAA TGGTGGTACG ATAAAGGGGA ATGTGGAAGC 
AAGGACTCCG GAAGTAAGGA CAAGACCAGT GCTCTGAGCC TGAGCAATGT GGCAGGCGTG 
TTCTACATCC TGATTGGAGG GCTGGGATTG GCCATGCTGG TTGCCTTAAT CGAGTTCTGC 
TACAAATCCC GTAGCGAGTC GAAGCGGATG AAGGGTTTCT GTTTGATTCC ACAGCAATCC 
ATCAATGAAG CCATACGGAC ATCGACCCTC CCCAGGAACA GCGGGGCAGG AGCCAGCGGA 
GGAAGTGGCA GTGGAGAGAA TGGCAGAGTG GTCAGCCAGG ACTTCCCCAA GTCCATGCAA 
TCCATTCCCT GCATGAGCCA CAGTTCAGGG ATGCCCTTGG GAGCCACAGG ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gria1 ( NM_001113325.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001106796.1 Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant 1, mRNA.

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CloneID	OMu23143
Clone ID Related Accession (Same CDS sequence)	NM_001113325.2
Accession Version	NM_001113325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	glutamate receptor 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL663055.13, AK049958.1 and BC056397.1. On Nov 18, 2011 this sequence version replaced NM_001113325.1. Transcript Variant: This variant (1) uses an alternate in-frame exon, compared to variant 2, resulting in a protein (isoform 1) with a distinct internal segment compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.115175.1, AK049958.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGTACA TCTTTGCCTT TTTCTGCACC GGTTTTCTAG GTGCGGTTGT GGGTGCCAAT 
TTCCCCAACA ATATCCAGAT AGGGGGATTA TTTCCAAACC AACAATCACA GGAACATGCG 
GCTTTTAGGT TTGCTTTGTC ACAACTCACG GAGCCCCCCA AGCTGCTTCC CCAGATCGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACTTACCGAT TCTGTTCCCA GTTCTCCAAA 
GGAGTGTACG CCATCTTTGG ATTTTATGAA CGAAGGACTG TCAACATGCT GACCTCCTTC 
TGTGGGGCCC TCCATGTGTG CTTCATCACT CCAAGTTTTC CCGTTGACAC ATCCAATCAG 
TTTGTCCTTC AGCTGCGCCC GGAACTACAG GAAGCTCTCA TTAGCATTAT CGACCATTAC 
AAGTGGCAGA CTTTTGTCTA CATTTATGAT GCTGACCGGG GCCTGTCAGT CCTGCAGAGA 
GTCTTGGATA CAGCCGCCGA GAAGAACTGG CAGGTGACGG CTGTCAACAT TCTAACAACC 
ACGGAGGAAG GATACCGGAT GCTCTTTCAG GACCTGGAGA AGAAAAAGGA GAGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAC GCCATCCTGG GCCAGATTGT GAAGCTAGAA 
AAGAACGGCA TCGGGTACCA CTACATCCTC GCCAACCTGG GCTTCATGGA CATTGACTTA 
AATAAGTTCA AGGAGAGTGG AGCCAATGTG ACAGGTTTCC AACTGGTGAA CTACACAGAC 
ACGATCCCAG CCAGAATCAT GCAGCAGTGG AGGACAAGTG ACGCTCGGGA CCACACCAGG 
GTGGACTGGA AGAGGCCAAA GTACACTTCT GCTCTTACCT ATGATGGTGT GAAGGTGATG 
GCGGAGGCCT TCCAGAGCCT GCGGAGGCAG AGGATTGACA TATCCCGGCG AGGGAATGCT 
GGGGACTGTC TGGCTAACCC AGCTGTGCCC TGGGGCCAAG GGATCGACAT CCAGAGAGCC 
CTGCAGCAGG TGCGCTTTGA AGGTTTGACA GGAAATGTGC AGTTTAACGA GAAAGGGCGC 
CGGACCAACT ACACCCTCCA TGTGATCGAA ATGAAGCATG ATGGAATCCG CAAGATTGGT 
TACTGGAATG AAGATGATAA ATTTGTCCCC GCAGCCACGG ACGCTCAGGC TGGAGGGGAC 
AACTCAAGCG TCCAGAATAG AACCTACATC GTCACGACTA TCCTCGAAGA TCCTTACGTG 
ATGCTTAAAA AGAATGCCAA CCAATTTGAA GGCAATGACC GCTATGAGGG CTACTGCGTG 
GAACTGGCTG CGGAGATCGC CAAGCACGTG GGCTATTCCT ACCGACTTGA GATTGTCAGC 
GACGGCAAAT ACGGAGCCCG GGATCCTGAC ACAAAGGCCT GGAATGGCAT GGTGGGAGAG 
CTAGTCTATG GAAGAGCAGA TGTGGCGGTG GCCCCCTTGA CCATAACCTT GGTCCGGGAG 
GAAGTCATCG ACTTCTCCAA GCCATTCATG AGTTTGGGAA TCTCCATTAT GATTAAGAAG 
CCACAGAAGT CCAAGCCAGG TGTCTTCTCC TTTCTTGACC CTTTGGCCTA CGAGATCTGG 
ATGTGTATAG TGTTTGCCTA CATTGGAGTG AGCGTCGTCC TCTTCCTGGT CAGCCGTTTC 
AGTCCTTATG AATGGCACAG TGAAGAGTTT GAAGAAGGAC GAGATCAGAC AACCAGTGAC 
CAGTCAAATG AGTTTGGCAT ATTCAACAGC CTGTGGTTCT CGCTGGGGGC CTTCATGCAG 
CAAGGATGTG ACATTTCCCC CAGGTCCCTG TCTGGACGCA TCGTCGGCGG TGTCTGGTGG 
TTCTTCACTT TGATTATCAT CTCCTCATAC ACAGCCAACC TGGCTGCCTT CCTGACTGTG 
GAAAGGATGG TGTCTCCCAT CGAGAGTGCA GAGGACCTGG CAAAGCAGAC GGAAATTGCT 
TATGGGACAT TGGAAGCAGG ATCCACTAAG GAGTTCTTCA GGAGGTCTAA AATCGCTGTG 
TTTGAGAAGA TGTGGACATA CATGAAGTCT GCAGAACCGT CTGTGTTTGT TCGGACCACA 
GAGGAGGGCA TGATCAGAGT GAGAAAGTCT AAAGGCAAAT ATGCCTACCT CCTGGAGTCC 
ACCATGAATG AGTACATTGA GCAACGCAAG CCCTGTGACA CCATGAAAGT GGGAGGTAAC 
TTGGATTCCA AAGGCTATGG CATTGCAACA CCCAAGGGGT CCGCCCTGAG AGGTCCCGTA 
AACCTAGCGG TTTTGAAACT CAGTGAGCAA GGCGTCTTAG ACAAGCTGAA AAGCAAATGG 
TGGTACGATA AAGGGGAATG TGGAAGCAAG GACTCCGGAA GTAAGGACAA GACCAGTGCT 
CTGAGCCTGA GCAATGTGGC AGGCGTGTTC TACATCCTGA TTGGAGGGCT GGGATTGGCC 
ATGCTGGTTG CCTTAATCGA GTTCTGCTAC AAATCCCGTA GCGAGTCGAA GCGGATGAAG 
GGTTTCTGTT TGATTCCACA GCAATCCATC AATGAAGCCA TACGGACATC GACCCTCCCC 
AGGAACAGCG GGGCAGGAGC CAGCGGAGGA AGTGGCAGTG GAGAGAATGG CAGAGTGGTC 
AGCCAGGACT TCCCCAAGTC CATGCAATCC ATTCCCTGCA TGAGCCACAG TTCAGGGATG 
CCCTTGGGAG CCACAGGATT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106796.1
CDS	417..3140
Translation	MPYIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPVNLAVLKLSEQGVLDKLKSKWWYDKGECGSKDSGSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGSGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	NM_001113325.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113325.2

ORF Insert Sequence:

ATGCCGTACA TCTTTGCCTT TTTCTGCACC GGTTTTCTAG GTGCGGTTGT GGGTGCCAAT 
TTCCCCAACA ATATCCAGAT AGGGGGATTA TTTCCAAACC AACAATCACA GGAACATGCG 
GCTTTTAGGT TTGCTTTGTC ACAACTCACG GAGCCCCCCA AGCTGCTTCC CCAGATCGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACTTACCGAT TCTGTTCCCA GTTCTCCAAA 
GGAGTGTACG CCATCTTTGG ATTTTATGAA CGAAGGACTG TCAACATGCT GACCTCCTTC 
TGTGGGGCCC TCCATGTGTG CTTCATCACT CCAAGTTTTC CCGTTGACAC ATCCAATCAG 
TTTGTCCTTC AGCTGCGCCC GGAACTACAG GAAGCTCTCA TTAGCATTAT CGACCATTAC 
AAGTGGCAGA CTTTTGTCTA CATTTATGAT GCTGACCGGG GCCTGTCAGT CCTGCAGAGA 
GTCTTGGATA CAGCCGCCGA GAAGAACTGG CAGGTGACGG CTGTCAACAT TCTAACAACC 
ACGGAGGAAG GATACCGGAT GCTCTTTCAG GACCTGGAGA AGAAAAAGGA GAGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAC GCCATCCTGG GCCAGATTGT GAAGCTAGAA 
AAGAACGGCA TCGGGTACCA CTACATCCTC GCCAACCTGG GCTTCATGGA CATTGACTTA 
AATAAGTTCA AGGAGAGTGG AGCCAATGTG ACAGGTTTCC AACTGGTGAA CTACACAGAC 
ACGATCCCAG CCAGAATCAT GCAGCAGTGG AGGACAAGTG ACGCTCGGGA CCACACCAGG 
GTGGACTGGA AGAGGCCAAA GTACACTTCT GCTCTTACCT ATGATGGTGT GAAGGTGATG 
GCGGAGGCCT TCCAGAGCCT GCGGAGGCAG AGGATTGACA TATCCCGGCG AGGGAATGCT 
GGGGACTGTC TGGCTAACCC AGCTGTGCCC TGGGGCCAAG GGATCGACAT CCAGAGAGCC 
CTGCAGCAGG TGCGCTTTGA AGGTTTGACA GGAAATGTGC AGTTTAACGA GAAAGGGCGC 
CGGACCAACT ACACCCTCCA TGTGATCGAA ATGAAGCATG ATGGAATCCG CAAGATTGGT 
TACTGGAATG AAGATGATAA ATTTGTCCCC GCAGCCACGG ACGCTCAGGC TGGAGGGGAC 
AACTCAAGCG TCCAGAATAG AACCTACATC GTCACGACTA TCCTCGAAGA TCCTTACGTG 
ATGCTTAAAA AGAATGCCAA CCAATTTGAA GGCAATGACC GCTATGAGGG CTACTGCGTG 
GAACTGGCTG CGGAGATCGC CAAGCACGTG GGCTATTCCT ACCGACTTGA GATTGTCAGC 
GACGGCAAAT ACGGAGCCCG GGATCCTGAC ACAAAGGCCT GGAATGGCAT GGTGGGAGAG 
CTAGTCTATG GAAGAGCAGA TGTGGCGGTG GCCCCCTTGA CCATAACCTT GGTCCGGGAG 
GAAGTCATCG ACTTCTCCAA GCCATTCATG AGTTTGGGAA TCTCCATTAT GATTAAGAAG 
CCACAGAAGT CCAAGCCAGG TGTCTTCTCC TTTCTTGACC CTTTGGCCTA CGAGATCTGG 
ATGTGTATAG TGTTTGCCTA CATTGGAGTG AGCGTCGTCC TCTTCCTGGT CAGCCGTTTC 
AGTCCTTATG AATGGCACAG TGAAGAGTTT GAAGAAGGAC GAGATCAGAC AACCAGTGAC 
CAGTCAAATG AGTTTGGCAT ATTCAACAGC CTGTGGTTCT CGCTGGGGGC CTTCATGCAG 
CAAGGATGTG ACATTTCCCC CAGGTCCCTG TCTGGACGCA TCGTCGGCGG TGTCTGGTGG 
TTCTTCACTT TGATTATCAT CTCCTCATAC ACAGCCAACC TGGCTGCCTT CCTGACTGTG 
GAAAGGATGG TGTCTCCCAT CGAGAGTGCA GAGGACCTGG CAAAGCAGAC GGAAATTGCT 
TATGGGACAT TGGAAGCAGG ATCCACTAAG GAGTTCTTCA GGAGGTCTAA AATCGCTGTG 
TTTGAGAAGA TGTGGACATA CATGAAGTCT GCAGAACCGT CTGTGTTTGT TCGGACCACA 
GAGGAGGGCA TGATCAGAGT GAGAAAGTCT AAAGGCAAAT ATGCCTACCT CCTGGAGTCC 
ACCATGAATG AGTACATTGA GCAACGCAAG CCCTGTGACA CCATGAAAGT GGGAGGTAAC 
TTGGATTCCA AAGGCTATGG CATTGCAACA CCCAAGGGGT CCGCCCTGAG AGGTCCCGTA 
AACCTAGCGG TTTTGAAACT CAGTGAGCAA GGCGTCTTAG ACAAGCTGAA AAGCAAATGG 
TGGTACGATA AAGGGGAATG TGGAAGCAAG GACTCCGGAA GTAAGGACAA GACCAGTGCT 
CTGAGCCTGA GCAATGTGGC AGGCGTGTTC TACATCCTGA TTGGAGGGCT GGGATTGGCC 
ATGCTGGTTG CCTTAATCGA GTTCTGCTAC AAATCCCGTA GCGAGTCGAA GCGGATGAAG 
GGTTTCTGTT TGATTCCACA GCAATCCATC AATGAAGCCA TACGGACATC GACCCTCCCC 
AGGAACAGCG GGGCAGGAGC CAGCGGAGGA AGTGGCAGTG GAGAGAATGG CAGAGTGGTC 
AGCCAGGACT TCCCCAAGTC CATGCAATCC ATTCCCTGCA TGAGCCACAG TTCAGGGATG 
CCCTTGGGAG CCACAGGATT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gria1 ( XM_006532234.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532297.1 Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X1, mRNA.

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CloneID	OMu23170
Clone ID Related Accession (Same CDS sequence)	XM_006532234.3 , NM_001252403.1 , XM_006532234.4
Accession Version	XM_006532234.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glutamate receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532234.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTACC GATTCTGTTC CCAGTTCTCC AAAGGAGTGT ACGCCATCTT TGGATTTTAT 
GAACGAAGGA CTGTCAACAT GCTGACCTCC TTCTGTGGGG CCCTCCATGT GTGCTTCATC 
ACTCCAAGTT TTCCCGTTGA CACATCCAAT CAGTTTGTCC TTCAGCTGCG CCCGGAACTA 
CAGGAAGCTC TCATTAGCAT TATCGACCAT TACAAGTGGC AGACTTTTGT CTACATTTAT 
GATGCTGACC GGGGCCTGTC AGTCCTGCAG AGAGTCTTGG ATACAGCCGC CGAGAAGAAC 
TGGCAGGTGA CGGCTGTCAA CATTCTAACA ACCACGGAGG AAGGATACCG GATGCTCTTT 
CAGGACCTGG AGAAGAAAAA GGAGAGGCTG GTGGTGGTGG ACTGTGAATC AGAACGCCTC 
AACGCCATCC TGGGCCAGAT TGTGAAGCTA GAAAAGAACG GCATCGGGTA CCACTACATC 
CTCGCCAACC TGGGCTTCAT GGACATTGAC TTAAATAAGT TCAAGGAGAG TGGAGCCAAT 
GTGACAGGTT TCCAACTGGT GAACTACACA GACACGATCC CAGCCAGAAT CATGCAGCAG 
TGGAGGACAA GTGACGCTCG GGACCACACC AGGGTGGACT GGAAGAGGCC AAAGTACACT 
TCTGCTCTTA CCTATGATGG TGTGAAGGTG ATGGCGGAGG CCTTCCAGAG CCTGCGGAGG 
CAGAGGATTG ACATATCCCG GCGAGGGAAT GCTGGGGACT GTCTGGCTAA CCCAGCTGTG 
CCCTGGGGCC AAGGGATCGA CATCCAGAGA GCCCTGCAGC AGGTGCGCTT TGAAGGTTTG 
ACAGGAAATG TGCAGTTTAA CGAGAAAGGG CGCCGGACCA ACTACACCCT CCATGTGATC 
GAAATGAAGC ATGATGGAAT CCGCAAGATT GGTTACTGGA ATGAAGATGA TAAATTTGTC 
CCCGCAGCCA CGGACGCTCA GGCTGGAGGG GACAACTCAA GCGTCCAGAA TAGAACCTAC 
ATCGTCACGA CTATCCTCGA AGATCCTTAC GTGATGCTTA AAAAGAATGC CAACCAATTT 
GAAGGCAATG ACCGCTATGA GGGCTACTGC GTGGAACTGG CTGCGGAGAT CGCCAAGCAC 
GTGGGCTATT CCTACCGACT TGAGATTGTC AGCGACGGCA AATACGGAGC CCGGGATCCT 
GACACAAAGG CCTGGAATGG CATGGTGGGA GAGCTAGTCT ATGGAAGAGC AGATGTGGCG 
GTGGCCCCCT TGACCATAAC CTTGGTCCGG GAGGAAGTCA TCGACTTCTC CAAGCCATTC 
ATGAGTTTGG GAATCTCCAT TATGATTAAG AAGCCACAGA AGTCCAAGCC AGGTGTCTTC 
TCCTTTCTTG ACCCTTTGGC CTACGAGATC TGGATGTGTA TAGTGTTTGC CTACATTGGA 
GTGAGCGTCG TCCTCTTCCT GGTCAGCCGT TTCAGTCCTT ATGAATGGCA CAGTGAAGAG 
TTTGAAGAAG GACGAGATCA GACAACCAGT GACCAGTCAA ATGAGTTTGG CATATTCAAC 
AGCCTGTGGT TCTCGCTGGG GGCCTTCATG CAGCAAGGAT GTGACATTTC CCCCAGGTCC 
CTGTCTGGAC GCATCGTCGG CGGTGTCTGG TGGTTCTTCA CTTTGATTAT CATCTCCTCA 
TACACAGCCA ACCTGGCTGC CTTCCTGACT GTGGAAAGGA TGGTGTCTCC CATCGAGAGT 
GCAGAGGACC TGGCAAAGCA GACGGAAATT GCTTATGGGA CATTGGAAGC AGGATCCACT 
AAGGAGTTCT TCAGGAGGTC TAAAATCGCT GTGTTTGAGA AGATGTGGAC ATACATGAAG 
TCTGCAGAAC CGTCTGTGTT TGTTCGGACC ACAGAGGAGG GCATGATCAG AGTGAGAAAG 
TCTAAAGGCA AATATGCCTA CCTCCTGGAG TCCACCATGA ATGAGTACAT TGAGCAACGC 
AAGCCCTGTG ACACCATGAA AGTGGGAGGT AACTTGGATT CCAAAGGCTA TGGCATTGCA 
ACACCCAAGG GGTCCGCCCT GAGAGGTCCC GTAAACCTAG CGGTTTTGAA ACTCAGTGAG 
CAAGGCGTCT TAGACAAGCT GAAAAGCAAA TGGTGGTACG ATAAAGGGGA ATGTGGAAGC 
AAGGACTCCG GAAGTAAGGA CAAGACCAGT GCTCTGAGCC TGAGCAATGT GGCAGGCGTG 
TTCTACATCC TGATTGGAGG GCTGGGATTG GCCATGCTGG TTGCCTTAAT CGAGTTCTGC 
TACAAATCCC GTAGCGAGTC GAAGCGGATG AAGGGTTTCT GTTTGATTCC ACAGCAATCC 
ATCAATGAAG CCATACGGAC ATCGACCCTC CCCAGGAACA GCGGGGCAGG AGCCAGCGGA 
GGAAGTGGCA GTGGAGAGAA TGGCAGAGTG GTCAGCCAGG ACTTCCCCAA GTCCATGCAA 
TCCATTCCCT GCATGAGCCA CAGTTCAGGG ATGCCCTTGG GAGCCACAGG ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532297.1
CDS	527..3043
Translation	MTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPVNLAVLKLSEQGVLDKLKSKWWYDKGECGSKDSGSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGSGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	XM_006532234.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532234.4

ORF Insert Sequence:

ATGACTTACC GATTCTGTTC CCAGTTCTCC AAAGGAGTGT ACGCCATCTT TGGATTTTAT 
GAACGAAGGA CTGTCAACAT GCTGACCTCC TTCTGTGGGG CCCTCCATGT GTGCTTCATC 
ACTCCAAGTT TTCCCGTTGA CACATCCAAT CAGTTTGTCC TTCAGCTGCG CCCGGAACTA 
CAGGAAGCTC TCATTAGCAT TATCGACCAT TACAAGTGGC AGACTTTTGT CTACATTTAT 
GATGCTGACC GGGGCCTGTC AGTCCTGCAG AGAGTCTTGG ATACAGCCGC CGAGAAGAAC 
TGGCAGGTGA CGGCTGTCAA CATTCTAACA ACCACGGAGG AAGGATACCG GATGCTCTTT 
CAGGACCTGG AGAAGAAAAA GGAGAGGCTG GTGGTGGTGG ACTGTGAATC AGAACGCCTC 
AACGCCATCC TGGGCCAGAT TGTGAAGCTA GAAAAGAACG GCATCGGGTA CCACTACATC 
CTCGCCAACC TGGGCTTCAT GGACATTGAC TTAAATAAGT TCAAGGAGAG TGGAGCCAAT 
GTGACAGGTT TCCAACTGGT GAACTACACA GACACGATCC CAGCCAGAAT CATGCAGCAG 
TGGAGGACAA GTGACGCTCG GGACCACACC AGGGTGGACT GGAAGAGGCC AAAGTACACT 
TCTGCTCTTA CCTATGATGG TGTGAAGGTG ATGGCGGAGG CCTTCCAGAG CCTGCGGAGG 
CAGAGGATTG ACATATCCCG GCGAGGGAAT GCTGGGGACT GTCTGGCTAA CCCAGCTGTG 
CCCTGGGGCC AAGGGATCGA CATCCAGAGA GCCCTGCAGC AGGTGCGCTT TGAAGGTTTG 
ACAGGAAATG TGCAGTTTAA CGAGAAAGGG CGCCGGACCA ACTACACCCT CCATGTGATC 
GAAATGAAGC ATGATGGAAT CCGCAAGATT GGTTACTGGA ATGAAGATGA TAAATTTGTC 
CCCGCAGCCA CGGACGCTCA GGCTGGAGGG GACAACTCAA GCGTCCAGAA TAGAACCTAC 
ATCGTCACGA CTATCCTCGA AGATCCTTAC GTGATGCTTA AAAAGAATGC CAACCAATTT 
GAAGGCAATG ACCGCTATGA GGGCTACTGC GTGGAACTGG CTGCGGAGAT CGCCAAGCAC 
GTGGGCTATT CCTACCGACT TGAGATTGTC AGCGACGGCA AATACGGAGC CCGGGATCCT 
GACACAAAGG CCTGGAATGG CATGGTGGGA GAGCTAGTCT ATGGAAGAGC AGATGTGGCG 
GTGGCCCCCT TGACCATAAC CTTGGTCCGG GAGGAAGTCA TCGACTTCTC CAAGCCATTC 
ATGAGTTTGG GAATCTCCAT TATGATTAAG AAGCCACAGA AGTCCAAGCC AGGTGTCTTC 
TCCTTTCTTG ACCCTTTGGC CTACGAGATC TGGATGTGTA TAGTGTTTGC CTACATTGGA 
GTGAGCGTCG TCCTCTTCCT GGTCAGCCGT TTCAGTCCTT ATGAATGGCA CAGTGAAGAG 
TTTGAAGAAG GACGAGATCA GACAACCAGT GACCAGTCAA ATGAGTTTGG CATATTCAAC 
AGCCTGTGGT TCTCGCTGGG GGCCTTCATG CAGCAAGGAT GTGACATTTC CCCCAGGTCC 
CTGTCTGGAC GCATCGTCGG CGGTGTCTGG TGGTTCTTCA CTTTGATTAT CATCTCCTCA 
TACACAGCCA ACCTGGCTGC CTTCCTGACT GTGGAAAGGA TGGTGTCTCC CATCGAGAGT 
GCAGAGGACC TGGCAAAGCA GACGGAAATT GCTTATGGGA CATTGGAAGC AGGATCCACT 
AAGGAGTTCT TCAGGAGGTC TAAAATCGCT GTGTTTGAGA AGATGTGGAC ATACATGAAG 
TCTGCAGAAC CGTCTGTGTT TGTTCGGACC ACAGAGGAGG GCATGATCAG AGTGAGAAAG 
TCTAAAGGCA AATATGCCTA CCTCCTGGAG TCCACCATGA ATGAGTACAT TGAGCAACGC 
AAGCCCTGTG ACACCATGAA AGTGGGAGGT AACTTGGATT CCAAAGGCTA TGGCATTGCA 
ACACCCAAGG GGTCCGCCCT GAGAGGTCCC GTAAACCTAG CGGTTTTGAA ACTCAGTGAG 
CAAGGCGTCT TAGACAAGCT GAAAAGCAAA TGGTGGTACG ATAAAGGGGA ATGTGGAAGC 
AAGGACTCCG GAAGTAAGGA CAAGACCAGT GCTCTGAGCC TGAGCAATGT GGCAGGCGTG 
TTCTACATCC TGATTGGAGG GCTGGGATTG GCCATGCTGG TTGCCTTAAT CGAGTTCTGC 
TACAAATCCC GTAGCGAGTC GAAGCGGATG AAGGGTTTCT GTTTGATTCC ACAGCAATCC 
ATCAATGAAG CCATACGGAC ATCGACCCTC CCCAGGAACA GCGGGGCAGG AGCCAGCGGA 
GGAAGTGGCA GTGGAGAGAA TGGCAGAGTG GTCAGCCAGG ACTTCCCCAA GTCCATGCAA 
TCCATTCCCT GCATGAGCCA CAGTTCAGGG ATGCCCTTGG GAGCCACAGG ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gria1 ( XM_017314275.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169764.1 Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X2, mRNA.

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CloneID	OMu77744
Clone ID Related Accession (Same CDS sequence)	XM_017314275.1 , XM_017314275.2
Accession Version	XM_017314275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glutamate receptor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTACC GATTCTGTTC CCAGTTCTCC AAAGGAGTGT ACGCCATCTT TGGATTTTAT 
GAACGAAGGA CTGTCAACAT GCTGACCTCC TTCTGTGGGG CCCTCCATGT GTGCTTCATC 
ACTCCAAGTT TTCCCGTTGA CACATCCAAT CAGTTTGTCC TTCAGCTGCG CCCGGAACTA 
CAGGAAGCTC TCATTAGCAT TATCGACCAT TACAAGTGGC AGACTTTTGT CTACATTTAT 
GATGCTGACC GGGGCCTGTC AGTCCTGCAG AGAGTCTTGG ATACAGCCGC CGAGAAGAAC 
TGGCAGGTGA CGGCTGTCAA CATTCTAACA ACCACGGAGG AAGGATACCG GATGCTCTTT 
CAGGACCTGG AGAAGAAAAA GGAGAGGCTG GTGGTGGTGG ACTGTGAATC AGAACGCCTC 
AACGCCATCC TGGGCCAGAT TGTGAAGCTA GAAAAGAACG GCATCGGGTA CCACTACATC 
CTCGCCAACC TGGGCTTCAT GGACATTGAC TTAAATAAGT TCAAGGAGAG TGGAGCCAAT 
GTGACAGGTT TCCAACTGGT GAACTACACA GACACGATCC CAGCCAGAAT CATGCAGCAG 
TGGAGGACAA GTGACGCTCG GGACCACACC AGGGTGGACT GGAAGAGGCC AAAGTACACT 
TCTGCTCTTA CCTATGATGG TGTGAAGGTG ATGGCGGAGG CCTTCCAGAG CCTGCGGAGG 
CAGAGGATTG ACATATCCCG GCGAGGGAAT GCTGGGGACT GTCTGGCTAA CCCAGCTGTG 
CCCTGGGGCC AAGGGATCGA CATCCAGAGA GCCCTGCAGC AGGTGCGCTT TGAAGGTTTG 
ACAGGAAATG TGCAGTTTAA CGAGAAAGGG CGCCGGACCA ACTACACCCT CCATGTGATC 
GAAATGAAGC ATGATGGAAT CCGCAAGATT GGTTACTGGA ATGAAGATGA TAAATTTGTC 
CCCGCAGCCA CGGACGCTCA GGCTGGAGGG GACAACTCAA GCGTCCAGAA TAGAACCTAC 
ATCGTCACGA CTATCCTCGA AGATCCTTAC GTGATGCTTA AAAAGAATGC CAACCAATTT 
GAAGGCAATG ACCGCTATGA GGGCTACTGC GTGGAACTGG CTGCGGAGAT CGCCAAGCAC 
GTGGGCTATT CCTACCGACT TGAGATTGTC AGCGACGGCA AATACGGAGC CCGGGATCCT 
GACACAAAGG CCTGGAATGG CATGGTGGGA GAGCTAGTCT ATGGAAGAGC AGATGTGGCG 
GTGGCCCCCT TGACCATAAC CTTGGTCCGG GAGGAAGTCA TCGACTTCTC CAAGCCATTC 
ATGAGTTTGG GAATCTCCAT TATGATTAAG AAGCCACAGA AGTCCAAGCC AGGTGTCTTC 
TCCTTTCTTG ACCCTTTGGC CTACGAGATC TGGATGTGTA TAGTGTTTGC CTACATTGGA 
GTGAGCGTCG TCCTCTTCCT GGTCAGCCGT TTCAGTCCTT ATGAATGGCA CAGTGAAGAG 
TTTGAAGAAG GACGAGATCA GACAACCAGT GACCAGTCAA ATGAGTTTGG CATATTCAAC 
AGCCTGTGGT TCTCGCTGGG GGCCTTCATG CAGCAAGGAT GTGACATTTC CCCCAGGTCC 
CTGTCTGGAC GCATCGTCGG CGGTGTCTGG TGGTTCTTCA CTTTGATTAT CATCTCCTCA 
TACACAGCCA ACCTGGCTGC CTTCCTGACT GTGGAAAGGA TGGTGTCTCC CATCGAGAGT 
GCAGAGGACC TGGCAAAGCA GACGGAAATT GCTTATGGGA CATTGGAAGC AGGATCCACT 
AAGGAGTTCT TCAGGAGGTC TAAAATCGCT GTGTTTGAGA AGATGTGGAC ATACATGAAG 
TCTGCAGAAC CGTCTGTGTT TGTTCGGACC ACAGAGGAGG GCATGATCAG AGTGAGAAAG 
TCTAAAGGCA AATATGCCTA CCTCCTGGAG TCCACCATGA ATGAGTACAT TGAGCAACGC 
AAGCCCTGTG ACACCATGAA AGTGGGAGGT AACTTGGATT CCAAAGGCTA TGGCATTGCA 
ACACCCAAGG GGTCCGCCCT GAGAAATCCA GTAAACCTGG CAGTGTTAAA ACTGAACGAG 
CAGGGGCTTT TGGACAAATT GAAAAACAAA TGGTGGTACG ACAAGGGCGA GTGCGGCAGC 
GGGGGAGGTG ACTCCAAGGA CAAGACCAGT GCTCTGAGCC TGAGCAATGT GGCAGGCGTG 
TTCTACATCC TGATTGGAGG GCTGGGATTG GCCATGCTGG TTGCCTTAAT CGAGTTCTGC 
TACAAATCCC GTAGCGAGTC GAAGCGGATG AAGGGTTTCT GTTTGATTCC ACAGCAATCC 
ATCAATGAAG CCATACGGAC ATCGACCCTC CCCAGGAACA GCGGGGCAGG AGCCAGCGGA 
GGAAGTGGCA GTGGAGAGAA TGGCAGAGTG GTCAGCCAGG ACTTCCCCAA GTCCATGCAA 
TCCATTCCCT GCATGAGCCA CAGTTCAGGG ATGCCCTTGG GAGCCACAGG ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169764.1
CDS	529..3045
Translation	MTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGSGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	XM_017314275.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314275.2

ORF Insert Sequence:

ATGACTTACC GATTCTGTTC CCAGTTCTCC AAAGGAGTGT ACGCCATCTT TGGATTTTAT 
GAACGAAGGA CTGTCAACAT GCTGACCTCC TTCTGTGGGG CCCTCCATGT GTGCTTCATC 
ACTCCAAGTT TTCCCGTTGA CACATCCAAT CAGTTTGTCC TTCAGCTGCG CCCGGAACTA 
CAGGAAGCTC TCATTAGCAT TATCGACCAT TACAAGTGGC AGACTTTTGT CTACATTTAT 
GATGCTGACC GGGGCCTGTC AGTCCTGCAG AGAGTCTTGG ATACAGCCGC CGAGAAGAAC 
TGGCAGGTGA CGGCTGTCAA CATTCTAACA ACCACGGAGG AAGGATACCG GATGCTCTTT 
CAGGACCTGG AGAAGAAAAA GGAGAGGCTG GTGGTGGTGG ACTGTGAATC AGAACGCCTC 
AACGCCATCC TGGGCCAGAT TGTGAAGCTA GAAAAGAACG GCATCGGGTA CCACTACATC 
CTCGCCAACC TGGGCTTCAT GGACATTGAC TTAAATAAGT TCAAGGAGAG TGGAGCCAAT 
GTGACAGGTT TCCAACTGGT GAACTACACA GACACGATCC CAGCCAGAAT CATGCAGCAG 
TGGAGGACAA GTGACGCTCG GGACCACACC AGGGTGGACT GGAAGAGGCC AAAGTACACT 
TCTGCTCTTA CCTATGATGG TGTGAAGGTG ATGGCGGAGG CCTTCCAGAG CCTGCGGAGG 
CAGAGGATTG ACATATCCCG GCGAGGGAAT GCTGGGGACT GTCTGGCTAA CCCAGCTGTG 
CCCTGGGGCC AAGGGATCGA CATCCAGAGA GCCCTGCAGC AGGTGCGCTT TGAAGGTTTG 
ACAGGAAATG TGCAGTTTAA CGAGAAAGGG CGCCGGACCA ACTACACCCT CCATGTGATC 
GAAATGAAGC ATGATGGAAT CCGCAAGATT GGTTACTGGA ATGAAGATGA TAAATTTGTC 
CCCGCAGCCA CGGACGCTCA GGCTGGAGGG GACAACTCAA GCGTCCAGAA TAGAACCTAC 
ATCGTCACGA CTATCCTCGA AGATCCTTAC GTGATGCTTA AAAAGAATGC CAACCAATTT 
GAAGGCAATG ACCGCTATGA GGGCTACTGC GTGGAACTGG CTGCGGAGAT CGCCAAGCAC 
GTGGGCTATT CCTACCGACT TGAGATTGTC AGCGACGGCA AATACGGAGC CCGGGATCCT 
GACACAAAGG CCTGGAATGG CATGGTGGGA GAGCTAGTCT ATGGAAGAGC AGATGTGGCG 
GTGGCCCCCT TGACCATAAC CTTGGTCCGG GAGGAAGTCA TCGACTTCTC CAAGCCATTC 
ATGAGTTTGG GAATCTCCAT TATGATTAAG AAGCCACAGA AGTCCAAGCC AGGTGTCTTC 
TCCTTTCTTG ACCCTTTGGC CTACGAGATC TGGATGTGTA TAGTGTTTGC CTACATTGGA 
GTGAGCGTCG TCCTCTTCCT GGTCAGCCGT TTCAGTCCTT ATGAATGGCA CAGTGAAGAG 
TTTGAAGAAG GACGAGATCA GACAACCAGT GACCAGTCAA ATGAGTTTGG CATATTCAAC 
AGCCTGTGGT TCTCGCTGGG GGCCTTCATG CAGCAAGGAT GTGACATTTC CCCCAGGTCC 
CTGTCTGGAC GCATCGTCGG CGGTGTCTGG TGGTTCTTCA CTTTGATTAT CATCTCCTCA 
TACACAGCCA ACCTGGCTGC CTTCCTGACT GTGGAAAGGA TGGTGTCTCC CATCGAGAGT 
GCAGAGGACC TGGCAAAGCA GACGGAAATT GCTTATGGGA CATTGGAAGC AGGATCCACT 
AAGGAGTTCT TCAGGAGGTC TAAAATCGCT GTGTTTGAGA AGATGTGGAC ATACATGAAG 
TCTGCAGAAC CGTCTGTGTT TGTTCGGACC ACAGAGGAGG GCATGATCAG AGTGAGAAAG 
TCTAAAGGCA AATATGCCTA CCTCCTGGAG TCCACCATGA ATGAGTACAT TGAGCAACGC 
AAGCCCTGTG ACACCATGAA AGTGGGAGGT AACTTGGATT CCAAAGGCTA TGGCATTGCA 
ACACCCAAGG GGTCCGCCCT GAGAAATCCA GTAAACCTGG CAGTGTTAAA ACTGAACGAG 
CAGGGGCTTT TGGACAAATT GAAAAACAAA TGGTGGTACG ACAAGGGCGA GTGCGGCAGC 
GGGGGAGGTG ACTCCAAGGA CAAGACCAGT GCTCTGAGCC TGAGCAATGT GGCAGGCGTG 
TTCTACATCC TGATTGGAGG GCTGGGATTG GCCATGCTGG TTGCCTTAAT CGAGTTCTGC 
TACAAATCCC GTAGCGAGTC GAAGCGGATG AAGGGTTTCT GTTTGATTCC ACAGCAATCC 
ATCAATGAAG CCATACGGAC ATCGACCCTC CCCAGGAACA GCGGGGCAGG AGCCAGCGGA 
GGAAGTGGCA GTGGAGAGAA TGGCAGAGTG GTCAGCCAGG ACTTCCCCAA GTCCATGCAA 
TCCATTCCCT GCATGAGCCA CAGTTCAGGG ATGCCCTTGG GAGCCACAGG ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Gria1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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サポート

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