Tle5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tle5
Entrez Gene ID	14797
Full Name	amino-terminal enhancer of split
Synonyms	AL024115,Esp1,Grg,Grg-5,Grg5,Tle5
General protein information	Preferred Names amino-terminal enhancer of split Names amino-terminal enhancer of split groucho-related protein 5 related to Drosophila groucho
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 C1\|10 39.72 cM
Summary	This gene encodes a protein that belongs to the Aes (amino-terminal enhancer of split) subgroup of the Groucho/transducin-like Enhancer of split (TLE) family of proteins that function as transcriptional corepressors. The encoded protein plays a role in neurological development and cell-fate determination. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010347.4	NP_034477.1	TLE family member 5 isoform 1
NM_001276288.1	NP_001263217.1	TLE family member 5 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	AES	NP_945320.1
Bos taurus (cattle)	AES	NP_001121969.1
Mus musculus (house mouse)	Aes	NP_034477.1
Rattus norvegicus (Norway rat)	Aes	NP_062093.1
Gallus gallus (chicken)	AES	XP_003642896.2
	LOC712353	XP_001099930.2
Pan troglodytes (chimpanzee)	AES	XP_001153657.1
Xenopus tropicalis (tropical clawed frog)	aes	NP_001039102.1
Danio rerio (zebrafish)	aes	NP_956717.1
Danio rerio (zebrafish)	chico	NP_570986.1

Gene Ontology
Bibliography

Related articles in PubMed

AML1-ETO requires enhanced C/D box snoRNA/RNP formation to induce self-renewal and leukaemia.
Zhou F, Liu Y, Rohde C, Pauli C, Gerloff D, K?hn M, Misiak D, B?umer N, Cui C, G?llner S, Oellerich T, Serve H, Garcia-Cuellar MP, Slany R, Maciejewski JP, Przychodzen B, Seliger B, Klein HU, Bartenhagen C, Berdel WE, Dugas M, Taketo MM, Farouq D, Schwartz S, Regev A, H?bert J, Sauvageau G, Pabst C, H?ttelmaier S, M?ller-Tidow C
Nature cell biology19(7)844-855(2017 Jul)

Expression of metastasis suppressor gene AES driven by a Yin Yang (YY) element in a CpG island promoter and transcription factor YY2.
Kakizaki F, Sonoshita M, Miyoshi H, Itatani Y, Ito S, Kawada K, Sakai Y, Taketo MM
Cancer science107(11)1622-1631(2016 Nov)

Disrupting Foxh1-Groucho interaction reveals robustness of nodal-based embryonic patterning.
Halstead AM, Wright CV
Mechanisms of development136155-65(2015 May)

Promotion of colorectal cancer invasion and metastasis through activation of NOTCH-DAB1-ABL-RHOGEF protein TRIO.
Sonoshita M, Itatani Y, Kakizaki F, Sakimura K, Terashima T, Katsuyama Y, Sakai Y, Taketo MM
Cancer discovery5(2)198-211(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in a transplantation mouse model of liver metastases, expression of Yy2 (and therefore of Aes) is downregulated
Title: Expression of metastasis suppressor gene AES driven by a Yin Yang (YY) element in a CpG island promoter and transcription factor YY2.

The finding that Foxh1-Grg-mediated repression is not essential for Nodal expression during mouse embryogenesis suggests that other regulators compensate for the loss of repressive regulatory input that is mediated by Grg interactions.
Title: Disrupting Foxh1-Groucho interaction reveals robustness of nodal-based embryonic patterning.

Self-renewal induced by AML1/ETO in primary murine progenitors was inhibited when Aes was decreased or absent.
Title: AML1/ETO induces self-renewal in hematopoietic progenitor cells via the Groucho-related amino-terminal AES protein.

The Aes gene functions as an endogenous metastasis suppressor. Aes inhibits Notch signaling by converting active Rbpj transcription complexes into repression complexes on insoluble nuclear matrix.
Title: Suppression of colon cancer metastasis by Aes through inhibition of Notch signaling.

direct interaction between Otx2 and Grg co-repressors regulates GnRH gene expression in hypothalamic neurons
Title: Otx2 induction of the gonadotropin-releasing hormone promoter is modulated by direct interactions with Grg co-repressors.

The following Tle5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tle5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06003	NM_010347.4 Latest version!	Mus musculus TLE family member 5, transcriptional modulator (Tle5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu07520	NM_001276288.1 Latest version!	Mus musculus TLE family member 5, transcriptional modulator (Tle5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tle5 ( NM_010347.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034477.1 Mus musculus TLE family member 5, transcriptional modulator (Tle5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06003
Clone ID Related Accession (Same CDS sequence)	NM_010347.4
Accession Version	NM_010347.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	TLE family member 5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY713872.1, AK158714.1 and BQ174071.1. On Jan 25, 2013 this sequence version replaced NM_010347.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131994.1, AK158714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATGTTTC CGCAAAGCCG GCACTCGGGC TCCTCCCACC TCCCTCAGCA GCTCAAGTTC 
ACCACCTCCG ACTCCTGTGA CCGCATCAAA GATGAGTTCC AGCTGCTGCA AGCGCAGTAT 
CACAGCCTGA AGCTGGAGTG CGACAAGCTG GCCAGCGAGA AGTCAGAGAT GCAGAGGCAT 
TACGTCATGT ACTATGAGAT GTCCTACGGA TTGAACATCG AGATGCACAA ACAGGCGGAA 
ATTGTGAAGA GGCTGAATGG GATTTGCGCC CAGGTTCTGC CCTATTTGTC ACAGGAGCAT 
CAGCAGCAGG TCCTGGGAGC CATCGAGAGA GCCAAGCAGG TCACGGCTCC TGAGCTGAAC 
TCCATCATCC GACAGCAGCT CCAGGCTCAC CAGCTGTCCC AGCTGCAGGC GCTGGCCCTG 
CCCCTGACAC CGCTGCCTGT CGGGCTCCAG CCACCGTCCC TCCCTGCAGT CAGTGCAGGC 
ACAGGCCTGC TGTCACTCTC TGCTCTGGGT TCTCAGACCC ACCTCTCCAA GGAGGACAAG 
AACGGACACG ATGGGGACAC CCACCAGGAG GATGACGGAG AGAAGTCGGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034477.1
CDS	199..792
Translation	MMFPQSRHSGSSHLPQQLKFTTSDSCDRIKDEFQLLQAQYHSLKLECDKLASEKSEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNGICAQVLPYLSQEHQQQVLGAIERAKQVTAPELNSIIRQQLQAHQLSQLQALALPLTPLPVGLQPPSLPAVSAGTGLLSLSALGSQTHLSKEDKNGHDGDTHQEDDGEKSD

Target ORF information:

RefSeq Version	NM_010347.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus TLE family member 5, transcriptional modulator (Tle5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010347.4

ORF Insert Sequence:

ATGATGTTTC CGCAAAGCCG GCACTCGGGC TCCTCCCACC TCCCTCAGCA GCTCAAGTTC 
ACCACCTCCG ACTCCTGTGA CCGCATCAAA GATGAGTTCC AGCTGCTGCA AGCGCAGTAT 
CACAGCCTGA AGCTGGAGTG CGACAAGCTG GCCAGCGAGA AGTCAGAGAT GCAGAGGCAT 
TACGTCATGT ACTATGAGAT GTCCTACGGA TTGAACATCG AGATGCACAA ACAGGCGGAA 
ATTGTGAAGA GGCTGAATGG GATTTGCGCC CAGGTTCTGC CCTATTTGTC ACAGGAGCAT 
CAGCAGCAGG TCCTGGGAGC CATCGAGAGA GCCAAGCAGG TCACGGCTCC TGAGCTGAAC 
TCCATCATCC GACAGCAGCT CCAGGCTCAC CAGCTGTCCC AGCTGCAGGC GCTGGCCCTG 
CCCCTGACAC CGCTGCCTGT CGGGCTCCAG CCACCGTCCC TCCCTGCAGT CAGTGCAGGC 
ACAGGCCTGC TGTCACTCTC TGCTCTGGGT TCTCAGACCC ACCTCTCCAA GGAGGACAAG 
AACGGACACG ATGGGGACAC CCACCAGGAG GATGACGGAG AGAAGTCGGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tle5 ( NM_001276288.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001263217.1 Mus musculus TLE family member 5, transcriptional modulator (Tle5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07520
Clone ID Related Accession (Same CDS sequence)	NM_001276288.1
Accession Version	NM_001276288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	TLE family member 5 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY713872.1, BC145299.1, AK158714.1 and BQ174071.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI653522.1, BC145299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATGTTTC CGCAAAGCCG GCACTCGGGC TCCTCCCACC TCCCTCAGCA GCTCAAGTTC 
ACCACCTCCG ACTCCTGTGA CCGCATCAAA GATGAGTTCC AGCTGCTGCA AGCGCAGTAT 
CACAGCCTGA AGCTGGAGTG CGACAAGCTG GCCAGCGAGA AGTCAGAGAT GCAGAGGCAT 
TACGTCATGT ACTATGAGAT GTCCTACGGA TTGAACATCG AGATGCACAA ACAGGCGGAA 
ATTGTGAAGA GGCTGAATGG GATTTGCGCC CAGGTTCTGC CCTATTTGTC ACAGGAGCAT 
CAGCAGCAGG TCCTGGGAGC CATCGAGAGA GCCAAGCAGG TCACGGCTCC TGAGCTGAAC 
TCCATCATCC GACAGCTCCA GGCTCACCAG CTGTCCCAGC TGCAGGCGCT GGCCCTGCCC 
CTGACACCGC TGCCTGTCGG GCTCCAGCCA CCGTCCCTCC CTGCAGTCAG TGCAGGCACA 
GGCCTGCTGT CACTCTCTGC TCTGGGTTCT CAGACCCACC TCTCCAAGGA GGACAAGAAC 
GGACACGATG GGGACACCCA CCAGGAGGAT GACGGAGAGA AGTCGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263217.1
CDS	199..789
Translation	MMFPQSRHSGSSHLPQQLKFTTSDSCDRIKDEFQLLQAQYHSLKLECDKLASEKSEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNGICAQVLPYLSQEHQQQVLGAIERAKQVTAPELNSIIRQLQAHQLSQLQALALPLTPLPVGLQPPSLPAVSAGTGLLSLSALGSQTHLSKEDKNGHDGDTHQEDDGEKSD

Target ORF information:

RefSeq Version	NM_001276288.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TLE family member 5, transcriptional modulator (Tle5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276288.1

ORF Insert Sequence:

ATGATGTTTC CGCAAAGCCG GCACTCGGGC TCCTCCCACC TCCCTCAGCA GCTCAAGTTC 
ACCACCTCCG ACTCCTGTGA CCGCATCAAA GATGAGTTCC AGCTGCTGCA AGCGCAGTAT 
CACAGCCTGA AGCTGGAGTG CGACAAGCTG GCCAGCGAGA AGTCAGAGAT GCAGAGGCAT 
TACGTCATGT ACTATGAGAT GTCCTACGGA TTGAACATCG AGATGCACAA ACAGGCGGAA 
ATTGTGAAGA GGCTGAATGG GATTTGCGCC CAGGTTCTGC CCTATTTGTC ACAGGAGCAT 
CAGCAGCAGG TCCTGGGAGC CATCGAGAGA GCCAAGCAGG TCACGGCTCC TGAGCTGAAC 
TCCATCATCC GACAGCTCCA GGCTCACCAG CTGTCCCAGC TGCAGGCGCT GGCCCTGCCC 
CTGACACCGC TGCCTGTCGG GCTCCAGCCA CCGTCCCTCC CTGCAGTCAG TGCAGGCACA 
GGCCTGCTGT CACTCTCTGC TCTGGGTTCT CAGACCCACC TCTCCAAGGA GGACAAGAAC 
GGACACGATG GGGACACCCA CCAGGAGGAT GACGGAGAGA AGTCGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

AML1-ETO requires enhanced C/D box snoRNA/RNP formation to induce self-renewal and leukaemia.
Nature cell biology19(7)844-855(2017 Jul)
Zhou F,Liu Y,Rohde C,Pauli C,Gerloff D,K?hn M,Misiak D,B?umer N,Cui C,G?llner S,Oellerich T,Serve H,Garcia-Cuellar MP,Slany R,Maciejewski JP,Przychodzen B,Seliger B,Klein HU,Bartenhagen C,Berdel WE,Dugas M,Taketo MM,Farouq D,Schwartz S,Regev A,H?bert J,Sauvageau G,Pabst C,H?ttelmaier S,M?ller-Tidow C

Expression of metastasis suppressor gene AES driven by a Yin Yang (YY) element in a CpG island promoter and transcription factor YY2.
Cancer science107(11)1622-1631(2016 Nov)
Kakizaki F,Sonoshita M,Miyoshi H,Itatani Y,Ito S,Kawada K,Sakai Y,Taketo MM

Disrupting Foxh1-Groucho interaction reveals robustness of nodal-based embryonic patterning.
Mechanisms of development136155-65(2015 May)
Halstead AM,Wright CV

Promotion of colorectal cancer invasion and metastasis through activation of NOTCH-DAB1-ABL-RHOGEF protein TRIO.
Cancer discovery5(2)198-211(2015 Feb)
Sonoshita M,Itatani Y,Kakizaki F,Sakimura K,Terashima T,Katsuyama Y,Sakai Y,Taketo MM

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Tle5 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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