Gpr50 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr50
Entrez Gene ID	14765
Full Name	G-protein-coupled receptor 50
Synonyms	H9
General protein information	Preferred Names G-protein-coupled receptor 50 Names melatonin-related receptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A7.3\|X 36.98 cM
Summary	This gene encodes a multipass membrane protein that is thought to act as a G protein-coupled receptor. Activity of this protein may be important in neurotransmitter and glucocorticoid signalling. Mutation of this gene causes a decreased ability to maintain a constant body temperature, resulting in torpor, as well as an increased metabolic rate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527827.3	XP_006527890.1	melatonin-related receptor isoform X1
NM_010340.2	NP_034470.1	melatonin-related receptor isoform 1
NM_001308501.1	NP_001295430.1	melatonin-related receptor isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPR50	NP_004215.2
	GPR50	XP_001092026.1
Rattus norvegicus (Norway rat)	Gpr50	NP_001178844.1
Bos taurus (cattle)	GPR50	XP_002699692.1
Mus musculus (house mouse)	Gpr50	NP_034470.1
Pan troglodytes (chimpanzee)	GPR50	XP_001136005.1
Canis lupus familiaris (dog)	GPR50	XP_549333.3

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

G protein coupled receptor 50 promotes self-renewal and neuronal differentiation of embryonic neural progenitor cells through regulation of notch and wnt/?-catenin signalings.
Ma YX, Wu ZQ, Feng YJ, Xiao ZC, Qin XL, Ma QH
Biochemical and biophysical research communications458(4)836-42(2015 Mar)

The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation.
Salvatierra J, Lee DA, Zibetti C, Duran-Moreno M, Yoo S, Newman EA, Wang H, Bedont JL, de Melo J, Miranda-Angulo AL, Gil-Perotin S, Garcia-Verdugo JM, Blackshaw S
The Journal of neuroscience : the official journal of the Society for Neuroscience34(50)16809-20(2014 Dec)

Induction of the metabolic regulator Txnip in fasting-induced and natural torpor.
Hand LE, Saer BR, Hui ST, Jinnah HA, Steinlechner S, Loudon AS, Bechtold DA
Endocrinology154(6)2081-91(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GPR50 promotes neuronal differentiation through its intracellular domain.
Title: G protein coupled receptor 50 promotes self-renewal and neuronal differentiation of embryonic neural progenitor cells through regulation of notch and wnt/?-catenin signalings.

Data indicate that Txnip expression is increased in the hypothalamus of Gpr50-/- mice during torpor.
Title: Induction of the metabolic regulator Txnip in fasting-induced and natural torpor.

A link is identified between GPR50 expression and neurotransmitter signaling during brain development.
Title: Developmental expression of orphan G protein-coupled receptor 50 in the mouse brain.

An enlarged distribution of GPR50 protein in the brain.
Title: Neuroanatomical distribution of the orphan GPR50 receptor in adult sheep and rodent brains.

GPR50 is a component of adaptive thermogenesis in mice
Title: A role for the melatonin-related receptor GPR50 in leptin signaling, adaptive thermogenesis, and torpor.

The following Gpr50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05988	NM_010340.2 Latest version!	Mus musculus G-protein-coupled receptor 50 (Gpr50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OMu67845	NM_001308501.1 Latest version!	Mus musculus G-protein-coupled receptor 50 (Gpr50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu56151	XM_006527827.3 Latest version!	Mus musculus G-protein-coupled receptor 50 (Gpr50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr50 ( NM_010340.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034470.1 Mus musculus G-protein-coupled receptor 50 (Gpr50), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05988
Clone ID Related Accession (Same CDS sequence)	NM_010340.2
Accession Version	NM_010340.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	melatonin-related receptor isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK132272.1, AL840624.14 and BQ032613.1. On May 8, 2015 this sequence version replaced NM_010340.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132272.1, BC137982.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACGG TCCCCAAGAG CAACATGGGA CCTACAAAGG CGGTTCCCAC CCCATTCGGC 
TGCATTGGCT GTAAGCTGCC AAAGCCCGAC TACCCGCCAG CTCTAATCAT CTTCATGTTC 
TGCGCAATGG TCATCACAGT CGTCGTAGAC CTGATCGGGA ACTCCATGGT CATTTTGGCT 
GTGACCAAGA ACAAGAAGCT CCGAAATTCT GGCAACATCT TTGTGGCCAG CCTCTCTGTG 
GCAGACATGC TGGTGGCCAT CTACCCCTAC CCTTTGATGC TGTATGCCAT GTCAGTTGGG 
GGCTGGGATC TGAGTCAGCT CCAGTGCCAG ATGGTCGGGT TGGTCACAGG ACTGAGCGTA 
GTCGGTTCCA TCTTCAACAT TACTGCCATT GCCATCAACC GTTACTGCTA CATCTGCCAC 
AGCCTCCAAT ACAAGCGGAT CTTCAGCCTG CGCAACACTT GCATCTATCT GGTCGTTACC 
TGGGTCATGA CTGTCCTGGC TGTCCTGCCT AACATGTACA TTGGCACCAT TGAGTATGAC 
CCTCGCACCT ACACCTGCAT CTTCAACTAT GTGAACAATC CTGCCTTTAC CGTGACCATT 
GTCTGCATCC ACTTCGTCCT CCCTCTCATC ATAGTTGGTT ATTGCTACAC GAAAATCTGG 
ATCAAAGTGC TGGCAGCCCG TGACCCAGCT GGACAGAATC CTGACAACCA GTTTGCTGAG 
GTTCGAAATT TTCTAACCAT GTTTGTGATC TTCCTCCTTT TTGCAGTGTG CTGGTGCCCT 
GTCAATGTGC TCACTGTGTT GGTGGCTGTC ATTCCAAAGG AAATGGCAGG CAAGATCCCC 
AACTGGCTTT ATCTTGCAGC CTACTGCATA GCCTACTTCA ACAGCTGCCT CAACGCCATC 
ATCTACGGTA TCCTCAATGA GAGTTTCCGA AGAGAATACT GGACCATCTT CCATGCTATG 
CGGCACCCTA TCCTGTTCAT CTCTCACCTC ATCAGTGATA TTCGGGAGAC TTGGGAGACC 
CGAGCTCTCA CTCGTGCCCG TGTCCGTGCC CGTGATCAAG TCCGAGAGCA AGAGCGTGCT 
CGTGCCTGTG TCGCTGTGGA GGGGACCCCA AGGAACGTCC GGAATGTTCT ACTGCCTGGT 
GATGCATCAG CACCCCACTC TGATCGTGCC TCTGTCCGTC CCAAGCCCCA AACCAGGTCT 
ACTTCTGTCT ACCGCAAACC TGCCTCTATC CACCACAAGT CTATTTCTGG CCACCCCAAG 
TCTGCCTCTG TTTACCCTAA GCCAGCCTCC TCTGTCCATT GCAAGCCTGC CTCTGTCCAT 
TTCAAACCCG CCTCTGTTCA TTTCAAGGGT GACTCTGTCT ATTTCAAGGG AGACACTGTC 
CATTACAGGG CTGCTTCCAA ACTTGTCACC AGTCACCGTA TCTCTGCTGG CCCTTCCACC 
AGTCACCCTA CATCCATGGC TGGCTACATT AAATCTGGTA CCAGCCACCC TGCCACCACC 
ACTGTTGACT ATCTCGAACC TGCCACCACC AGCCACTCTG TGCTCACTGC TGTCGACCTC 
CCTGAGGTCT CAGCCTCCCA TTGCCTTGAG ATGACCAGCA CTGGCCACCT CAGAGCTGAC 
ATTTCTGCCT CTGTCCTTCC TTCTGTACCC TTCGAGCTTG CTGCCACCCC TCCTGATACC 
ACTGCAATCC CCATTGCCTC TGGTGATTAC CGCAAGGTCG TGCTTATTGA TGATGATTCT 
GATGATTCTG ATTGCTCTGA TGAGATGGCT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034470.1
CDS	126..1901
Translation	MATVPKSNMGPTKAVPTPFGCIGCKLPKPDYPPALIIFMFCAMVITVVVDLIGNSMVILAVTKNKKLRNSGNIFVASLSVADMLVAIYPYPLMLYAMSVGGWDLSQLQCQMVGLVTGLSVVGSIFNITAIAINRYCYICHSLQYKRIFSLRNTCIYLVVTWVMTVLAVLPNMYIGTIEYDPRTYTCIFNYVNNPAFTVTIVCIHFVLPLIIVGYCYTKIWIKVLAARDPAGQNPDNQFAEVRNFLTMFVIFLLFAVCWCPVNVLTVLVAVIPKEMAGKIPNWLYLAAYCIAYFNSCLNAIIYGILNESFRREYWTIFHAMRHPILFISHLISDIRETWETRALTRARVRARDQVREQERARACVAVEGTPRNVRNVLLPGDASAPHSDRASVRPKPQTRSTSVYRKPASIHHKSISGHPKSASVYPKPASSVHCKPASVHFKPASVHFKGDSVYFKGDTVHYRAASKLVTSHRISAGPSTSHPTSMAGYIKSGTSHPATTTVDYLEPATTSHSVLTAVDLPEVSASHCLEMTSTGHLRADISASVLPSVPFELAATPPDTTAIPIASGDYRKVVLIDDDSDDSDCSDEMAV

Target ORF information:

RefSeq Version	NM_010340.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus G-protein-coupled receptor 50 (Gpr50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010340.2

ORF Insert Sequence:

ATGGCCACGG TCCCCAAGAG CAACATGGGA CCTACAAAGG CGGTTCCCAC CCCATTCGGC 
TGCATTGGCT GTAAGCTGCC AAAGCCCGAC TACCCGCCAG CTCTAATCAT CTTCATGTTC 
TGCGCAATGG TCATCACAGT CGTCGTAGAC CTGATCGGGA ACTCCATGGT CATTTTGGCT 
GTGACCAAGA ACAAGAAGCT CCGAAATTCT GGCAACATCT TTGTGGCCAG CCTCTCTGTG 
GCAGACATGC TGGTGGCCAT CTACCCCTAC CCTTTGATGC TGTATGCCAT GTCAGTTGGG 
GGCTGGGATC TGAGTCAGCT CCAGTGCCAG ATGGTCGGGT TGGTCACAGG ACTGAGCGTA 
GTCGGTTCCA TCTTCAACAT TACTGCCATT GCCATCAACC GTTACTGCTA CATCTGCCAC 
AGCCTCCAAT ACAAGCGGAT CTTCAGCCTG CGCAACACTT GCATCTATCT GGTCGTTACC 
TGGGTCATGA CTGTCCTGGC TGTCCTGCCT AACATGTACA TTGGCACCAT TGAGTATGAC 
CCTCGCACCT ACACCTGCAT CTTCAACTAT GTGAACAATC CTGCCTTTAC CGTGACCATT 
GTCTGCATCC ACTTCGTCCT CCCTCTCATC ATAGTTGGTT ATTGCTACAC GAAAATCTGG 
ATCAAAGTGC TGGCAGCCCG TGACCCAGCT GGACAGAATC CTGACAACCA GTTTGCTGAG 
GTTCGAAATT TTCTAACCAT GTTTGTGATC TTCCTCCTTT TTGCAGTGTG CTGGTGCCCT 
GTCAATGTGC TCACTGTGTT GGTGGCTGTC ATTCCAAAGG AAATGGCAGG CAAGATCCCC 
AACTGGCTTT ATCTTGCAGC CTACTGCATA GCCTACTTCA ACAGCTGCCT CAACGCCATC 
ATCTACGGTA TCCTCAATGA GAGTTTCCGA AGAGAATACT GGACCATCTT CCATGCTATG 
CGGCACCCTA TCCTGTTCAT CTCTCACCTC ATCAGTGATA TTCGGGAGAC TTGGGAGACC 
CGAGCTCTCA CTCGTGCCCG TGTCCGTGCC CGTGATCAAG TCCGAGAGCA AGAGCGTGCT 
CGTGCCTGTG TCGCTGTGGA GGGGACCCCA AGGAACGTCC GGAATGTTCT ACTGCCTGGT 
GATGCATCAG CACCCCACTC TGATCGTGCC TCTGTCCGTC CCAAGCCCCA AACCAGGTCT 
ACTTCTGTCT ACCGCAAACC TGCCTCTATC CACCACAAGT CTATTTCTGG CCACCCCAAG 
TCTGCCTCTG TTTACCCTAA GCCAGCCTCC TCTGTCCATT GCAAGCCTGC CTCTGTCCAT 
TTCAAACCCG CCTCTGTTCA TTTCAAGGGT GACTCTGTCT ATTTCAAGGG AGACACTGTC 
CATTACAGGG CTGCTTCCAA ACTTGTCACC AGTCACCGTA TCTCTGCTGG CCCTTCCACC 
AGTCACCCTA CATCCATGGC TGGCTACATT AAATCTGGTA CCAGCCACCC TGCCACCACC 
ACTGTTGACT ATCTCGAACC TGCCACCACC AGCCACTCTG TGCTCACTGC TGTCGACCTC 
CCTGAGGTCT CAGCCTCCCA TTGCCTTGAG ATGACCAGCA CTGGCCACCT CAGAGCTGAC 
ATTTCTGCCT CTGTCCTTCC TTCTGTACCC TTCGAGCTTG CTGCCACCCC TCCTGATACC 
ACTGCAATCC CCATTGCCTC TGGTGATTAC CGCAAGGTCG TGCTTATTGA TGATGATTCT 
GATGATTCTG ATTGCTCTGA TGAGATGGCT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr50 ( NM_001308501.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001295430.1 Mus musculus G-protein-coupled receptor 50 (Gpr50), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67845
Clone ID Related Accession (Same CDS sequence)	NM_001308501.1
Accession Version	NM_001308501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	melatonin-related receptor isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK132272.1, BC145080.1 and BQ032613.1. Transcript Variant: This variant (2) lacks an alternate segment, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145080.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACGG TCCCCAAGAG CAACATGGGA CCTACAAAGG CGGTTCCCAC CCCATTCGGC 
TGCATTGGCT GTAAGCTGCC AAAGCCCGAC TACCCGCCAG CTCTAATCAT CTTCATGTTC 
TGCGCAATGG TCATCACAGT CGTCGTAGAC CTGATCGGGA ACTCCATGGT CATTTTGGCT 
GTGACCAAGA ACAAGAAGCT CCGAAATTCT GGCAACATCT TTGTGGCCAG CCTCTCTGTG 
GCAGACATGC TGGTGGCCAT CTACCCCTAC CCTTTGATGC TGTATGCCAT GTCAGTTGGG 
GGCTGGGATC TGAGTCAGCT CCAGTGCCAG ATGGTCGGGT TGGTCACAGG ACTGAGCGTA 
GTCGGTTCCA TCTTCAACAT TACTGCCATT GCCATCAACC GTTACTGCTA CATCTGCCAC 
AGCCTCCAAT ACAAGCGGAT CTTCAGCCTG CGCAACACTT GCATCTATCT GGTCGTTACC 
TGGGTCATGA CTGTCCTGGC TGTCCTGCCT AACATGTACA TTGGCACCAT TGAGTATGAC 
CCTCGCACCT ACACCTGCAT CTTCAACTAT GTGAACAATC CTGCCTTTAC CGTGACCATT 
GTCTGCATCC ACTTCGTCCT CCCTCTCATC ATAGTTGGTT ATTGCTACAC GAAAATCTGG 
ATCAAAGTGC TGGCAGCCCG TGACCCAGCT GGACAGAATC CTGACAACCA GTTTGCTGAG 
CCTACTGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295430.1
CDS	126..857
Translation	MATVPKSNMGPTKAVPTPFGCIGCKLPKPDYPPALIIFMFCAMVITVVVDLIGNSMVILAVTKNKKLRNSGNIFVASLSVADMLVAIYPYPLMLYAMSVGGWDLSQLQCQMVGLVTGLSVVGSIFNITAIAINRYCYICHSLQYKRIFSLRNTCIYLVVTWVMTVLAVLPNMYIGTIEYDPRTYTCIFNYVNNPAFTVTIVCIHFVLPLIIVGYCYTKIWIKVLAARDPAGQNPDNQFAEPTA

Target ORF information:

RefSeq Version	NM_001308501.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus G-protein-coupled receptor 50 (Gpr50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308501.1

ORF Insert Sequence:

ATGGCCACGG TCCCCAAGAG CAACATGGGA CCTACAAAGG CGGTTCCCAC CCCATTCGGC 
TGCATTGGCT GTAAGCTGCC AAAGCCCGAC TACCCGCCAG CTCTAATCAT CTTCATGTTC 
TGCGCAATGG TCATCACAGT CGTCGTAGAC CTGATCGGGA ACTCCATGGT CATTTTGGCT 
GTGACCAAGA ACAAGAAGCT CCGAAATTCT GGCAACATCT TTGTGGCCAG CCTCTCTGTG 
GCAGACATGC TGGTGGCCAT CTACCCCTAC CCTTTGATGC TGTATGCCAT GTCAGTTGGG 
GGCTGGGATC TGAGTCAGCT CCAGTGCCAG ATGGTCGGGT TGGTCACAGG ACTGAGCGTA 
GTCGGTTCCA TCTTCAACAT TACTGCCATT GCCATCAACC GTTACTGCTA CATCTGCCAC 
AGCCTCCAAT ACAAGCGGAT CTTCAGCCTG CGCAACACTT GCATCTATCT GGTCGTTACC 
TGGGTCATGA CTGTCCTGGC TGTCCTGCCT AACATGTACA TTGGCACCAT TGAGTATGAC 
CCTCGCACCT ACACCTGCAT CTTCAACTAT GTGAACAATC CTGCCTTTAC CGTGACCATT 
GTCTGCATCC ACTTCGTCCT CCCTCTCATC ATAGTTGGTT ATTGCTACAC GAAAATCTGG 
ATCAAAGTGC TGGCAGCCCG TGACCCAGCT GGACAGAATC CTGACAACCA GTTTGCTGAG 
CCTACTGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr50 ( XM_006527827.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527890.1 Mus musculus G-protein-coupled receptor 50 (Gpr50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56151
Clone ID Related Accession (Same CDS sequence)	XM_006527827.3
Accession Version	XM_006527827.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	melatonin-related receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTGG CCATCTACCC CTACCCTTTG ATGCTGTATG CCATGTCAGT TGGGGGCTGG 
GATCTGAGTC AGCTCCAGTG CCAGATGGTC GGGTTGGTCA CAGGACTGAG CGTAGTCGGT 
TCCATCTTCA ACATTACTGC CATTGCCATC AACCGTTACT GCTACATCTG CCACAGCCTC 
CAATACAAGC GGATCTTCAG CCTGCGCAAC ACTTGCATCT ATCTGGTCGT TACCTGGGTC 
ATGACTGTCC TGGCTGTCCT GCCTAACATG TACATTGGCA CCATTGAGTA TGACCCTCGC 
ACCTACACCT GCATCTTCAA CTATGTGAAC AATCCTGCCT TTACCGTGAC CATTGTCTGC 
ATCCACTTCG TCCTCCCTCT CATCATAGTT GGTTATTGCT ACACGAAAAT CTGGATCAAA 
GTGCTGGCAG CCCGTGACCC AGCTGGACAG AATCCTGACA ACCAGTTTGC TGAGGTTCGA 
AATTTTCTAA CCATGTTTGT GATCTTCCTC CTTTTTGCAG TGTGCTGGTG CCCTGTCAAT 
GTGCTCACTG TGTTGGTGGC TGTCATTCCA AAGGAAATGG CAGGCAAGAT CCCCAACTGG 
CTTTATCTTG CAGCCTACTG CATAGCCTAC TTCAACAGCT GCCTCAACGC CATCATCTAC 
GGTATCCTCA ATGAGAGTTT CCGAAGAGAA TACTGGACCA TCTTCCATGC TATGCGGCAC 
CCTATCCTGT TCATCTCTCA CCTCATCAGT GATATTCGGG AGACTTGGGA GACCCGAGCT 
CTCACTCGTG CCCGTGTCCG TGCCCGTGAT CAAGTCCGAG AGCAAGAGCG TGCTCGTGCC 
TGTGTCGCTG TGGAGGGGAC CCCAAGGAAC GTCCGGAATG TTCTACTGCC TGGTGATGCA 
TCAGCACCCC ACTCTGATCG TGCCTCTGTC CGTCCCAAGC CCCAAACCAG GTCTACTTCT 
GTCTACCGCA AACCTGCCTC TATCCACCAC AAGTCTATTT CTGGCCACCC CAAGTCTGCC 
TCTGTTTACC CTAAGCCAGC CTCCTCTGTC CATTGCAAGC CTGCCTCTGT CCATTTCAAA 
CCCGCCTCTG TTCATTTCAA GGGTGACTCT GTCTATTTCA AGGGAGACAC TGTCCATTAC 
AGGGCTGCTT CCAAACTTGT CACCAGTCAC CGTATCTCTG CTGGCCCTTC CACCAGTCAC 
CCTACATCCA TGGCTGGCTA CATTAAATCT GGTACCAGCC ACCCTGCCAC CACCACTGTT 
GACTATCTCG AACCTGCCAC CACCAGCCAC TCTGTGCTCA CTGCTGTCGA CCTCCCTGAG 
GTCTCAGCCT CCCATTGCCT TGAGATGACC AGCACTGGCC ACCTCAGAGC TGACATTTCT 
GCCTCTGTCC TTCCTTCTGT ACCCTTCGAG CTTGCTGCCA CCCCTCCTGA TACCACTGCA 
ATCCCCATTG CCTCTGGTGA TTACCGCAAG GTCGTGCTTA TTGATGATGA TTCTGATGAT 
TCTGATTGCT CTGATGAGAT GGCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527890.1
CDS	649..2178
Translation	MLVAIYPYPLMLYAMSVGGWDLSQLQCQMVGLVTGLSVVGSIFNITAIAINRYCYICHSLQYKRIFSLRNTCIYLVVTWVMTVLAVLPNMYIGTIEYDPRTYTCIFNYVNNPAFTVTIVCIHFVLPLIIVGYCYTKIWIKVLAARDPAGQNPDNQFAEVRNFLTMFVIFLLFAVCWCPVNVLTVLVAVIPKEMAGKIPNWLYLAAYCIAYFNSCLNAIIYGILNESFRREYWTIFHAMRHPILFISHLISDIRETWETRALTRARVRARDQVREQERARACVAVEGTPRNVRNVLLPGDASAPHSDRASVRPKPQTRSTSVYRKPASIHHKSISGHPKSASVYPKPASSVHCKPASVHFKPASVHFKGDSVYFKGDTVHYRAASKLVTSHRISAGPSTSHPTSMAGYIKSGTSHPATTTVDYLEPATTSHSVLTAVDLPEVSASHCLEMTSTGHLRADISASVLPSVPFELAATPPDTTAIPIASGDYRKVVLIDDDSDDSDCSDEMAV

Target ORF information:

RefSeq Version	XM_006527827.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus G-protein-coupled receptor 50 (Gpr50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527827.3

ORF Insert Sequence:

ATGCTGGTGG CCATCTACCC CTACCCTTTG ATGCTGTATG CCATGTCAGT TGGGGGCTGG 
GATCTGAGTC AGCTCCAGTG CCAGATGGTC GGGTTGGTCA CAGGACTGAG CGTAGTCGGT 
TCCATCTTCA ACATTACTGC CATTGCCATC AACCGTTACT GCTACATCTG CCACAGCCTC 
CAATACAAGC GGATCTTCAG CCTGCGCAAC ACTTGCATCT ATCTGGTCGT TACCTGGGTC 
ATGACTGTCC TGGCTGTCCT GCCTAACATG TACATTGGCA CCATTGAGTA TGACCCTCGC 
ACCTACACCT GCATCTTCAA CTATGTGAAC AATCCTGCCT TTACCGTGAC CATTGTCTGC 
ATCCACTTCG TCCTCCCTCT CATCATAGTT GGTTATTGCT ACACGAAAAT CTGGATCAAA 
GTGCTGGCAG CCCGTGACCC AGCTGGACAG AATCCTGACA ACCAGTTTGC TGAGGTTCGA 
AATTTTCTAA CCATGTTTGT GATCTTCCTC CTTTTTGCAG TGTGCTGGTG CCCTGTCAAT 
GTGCTCACTG TGTTGGTGGC TGTCATTCCA AAGGAAATGG CAGGCAAGAT CCCCAACTGG 
CTTTATCTTG CAGCCTACTG CATAGCCTAC TTCAACAGCT GCCTCAACGC CATCATCTAC 
GGTATCCTCA ATGAGAGTTT CCGAAGAGAA TACTGGACCA TCTTCCATGC TATGCGGCAC 
CCTATCCTGT TCATCTCTCA CCTCATCAGT GATATTCGGG AGACTTGGGA GACCCGAGCT 
CTCACTCGTG CCCGTGTCCG TGCCCGTGAT CAAGTCCGAG AGCAAGAGCG TGCTCGTGCC 
TGTGTCGCTG TGGAGGGGAC CCCAAGGAAC GTCCGGAATG TTCTACTGCC TGGTGATGCA 
TCAGCACCCC ACTCTGATCG TGCCTCTGTC CGTCCCAAGC CCCAAACCAG GTCTACTTCT 
GTCTACCGCA AACCTGCCTC TATCCACCAC AAGTCTATTT CTGGCCACCC CAAGTCTGCC 
TCTGTTTACC CTAAGCCAGC CTCCTCTGTC CATTGCAAGC CTGCCTCTGT CCATTTCAAA 
CCCGCCTCTG TTCATTTCAA GGGTGACTCT GTCTATTTCA AGGGAGACAC TGTCCATTAC 
AGGGCTGCTT CCAAACTTGT CACCAGTCAC CGTATCTCTG CTGGCCCTTC CACCAGTCAC 
CCTACATCCA TGGCTGGCTA CATTAAATCT GGTACCAGCC ACCCTGCCAC CACCACTGTT 
GACTATCTCG AACCTGCCAC CACCAGCCAC TCTGTGCTCA CTGCTGTCGA CCTCCCTGAG 
GTCTCAGCCT CCCATTGCCT TGAGATGACC AGCACTGGCC ACCTCAGAGC TGACATTTCT 
GCCTCTGTCC TTCCTTCTGT ACCCTTCGAG CTTGCTGCCA CCCCTCCTGA TACCACTGCA 
ATCCCCATTG CCTCTGGTGA TTACCGCAAG GTCGTGCTTA TTGATGATGA TTCTGATGAT 
TCTGATTGCT CTGATGAGAT GGCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

G protein coupled receptor 50 promotes self-renewal and neuronal differentiation of embryonic neural progenitor cells through regulation of notch and wnt/?-catenin signalings.
Biochemical and biophysical research communications458(4)836-42(2015 Mar)
Ma YX,Wu ZQ,Feng YJ,Xiao ZC,Qin XL,Ma QH

The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(50)16809-20(2014 Dec)
Salvatierra J,Lee DA,Zibetti C,Duran-Moreno M,Yoo S,Newman EA,Wang H,Bedont JL,de Melo J,Miranda-Angulo AL,Gil-Perotin S,Garcia-Verdugo JM,Blackshaw S

Induction of the metabolic regulator Txnip in fasting-induced and natural torpor.
Endocrinology154(6)2081-91(2013 Jun)
Hand LE,Saer BR,Hui ST,Jinnah HA,Steinlechner S,Loudon AS,Bechtold DA

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Gpr50 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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