Gpi1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpi1
Entrez Gene ID	14751
Full Name	glucose phosphate isomerase 1
Synonyms	Amf,Gpi,Gpi-1,Gpi-1r,Gpi-1s,Gpi-1t,Gpi1-r,Gpi1-s,Gpi1-t,Gpi1s,MF,NK,NK/GPI,Nlk,Org,Pgi,Phi
General protein information	Preferred Names glucose phosphate isomerase 1 Names glucose-6-phosphate isomerase autocrine motility factor glucose phosphate isomerase 1 complex glucose phosphate isomerase 1, regulatory glucose phosphate isomerase 1, structural glucose phosphate isomerase 1, temporal maturation factor neuroleukin phosphoglucose isomerase phosphohexose isomerase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 B1\|7 19.46 cM
Summary	This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. [provided by RefSeq, Aug 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008155.4	NP_032181.2	glucose-6-phosphate isomerase

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPI	NP_000166.2
Rattus norvegicus (Norway rat)	Gpi	NP_997475.1
Drosophila melanogaster (fruit fly)	Pgi	NP_724724.1
Schizosaccharomyces pombe (fission yeast)	pgi1	NP_596635.1
Neurospora crassa	NCU07281	XP_962135.1
Arabidopsis thaliana (thale cress)	AT5G42740	NP_199088.1
Oryza sativa (rice)	Os06g0256500	NP_001057316.1
Xenopus tropicalis (tropical clawed frog)	gpi	NP_001011010.1
Mus musculus (house mouse)	Gpi1	NP_032181.2
Gallus gallus (chicken)	GPI	NP_001006128.1
Caenorhabditis elegans (roundworm)	gpi-1	NP_001021838.1
Kluyveromyces lactis	KLLA0E23519g	XP_455013.1
Eremothecium gossypii	AGOS_AEL249C	NP_984612.1
Magnaporthe oryzae (rice blast fungus)	MGG_12822	XP_003717069.1
Pan troglodytes (chimpanzee)	GPI	XP_003316332.1
Canis lupus familiaris (dog)	GPI	XP_853858.1
Danio rerio (zebrafish)	gpib	NP_658910.2
Oryza sativa (rice)	Os03g0776000	NP_001051434.1
Danio rerio (zebrafish)	gpia	NP_658909.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012167	XP_320366.3
Bos taurus (cattle)	GPI	NP_001035561.1
Saccharomyces cerevisiae (baker's yeast)	PGI1	NP_009755.1

Gene Ontology
Bibliography

Related articles in PubMed

Hierarchically related lineage-restricted fates of multipotent haematopoietic stem cells.
Carrelha J, Meng Y, Kettyle LM, Luis TC, Norfo R, Alcolea V, Grasso F, Gambardella A, Grover A, H?gstrand K, Lord AM, Sanjuan-Pla A, Woll PS, Nerlov C, Sten Eirik W J
Nature(2018 Jan)

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

Tumor cell autocrine motility factor is the neuroleukin/phosphohexose isomerase polypeptide.
Watanabe H, Takehana K, Date M, Shinozaki T, Raz A
Cancer research56(13)2960-3(1996 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mGPI-transgenic mice presented GPI more efficiently and showed a dramatic increase in negative selection and an inhibition of arthritis.
Title: Insufficient autoantigen presentation and failure of tolerance in a mouse model of rheumatoid arthritis.

Increased red cell turnover in a line of CD22-deficient mice is caused by Gpi1c: a model for hereditary haemolytic anaemia.
Title: Increased red cell turnover in a line of CD22-deficient mice is caused by Gpi1c: a model for hereditary haemolytic anaemia.

Data show that silencing of AMF/PGI induced MET and suppressed the tumor growth and pulmonary metastasis of LM8 cells in nude mice.
Title: Silencing of autocrine motility factor induces mesenchymal-to-epithelial transition and suppression of osteosarcoma pulmonary metastasis.

the receptor molecule for AMF/NLK/MF in leukemic differentiation is not gp78
Title: AMF/G6PI induces differentiation of leukemic cells via an unknown receptor that differs from gp78.

Results report the first high-resolution crystal structure of the inhibitor-free form and the eight types of inhibitor complexes of mouse autocrine motility factor.
Title: Crystal structures of mouse autocrine motility factor in complex with carbohydrate phosphate inhibitors provide insight into structure-activity relationship of the inhibitors.

The following Gpi1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpi1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16355	NM_008155.4 Latest version!	Mus musculus glucose-6-phosphate isomerase 1 (Gpi1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$328.30 $469.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpi1 ( NM_008155.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032181.2 Mus musculus glucose-6-phosphate isomerase 1 (Gpi1), mRNA.

Price & Availability↑

CloneID	OMu16355
Clone ID Related Accession (Same CDS sequence)	NM_008155.4
Accession Version	NM_008155.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	glucose-6-phosphate isomerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY350616.1, AK147124.1 and AK031081.1. On Jan 25, 2014 this sequence version replaced NM_008155.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031081.1, BC086640.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 3352745, 8674049 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGC TCACCCGGAA CCCGCAGTTC CAGAAGCTCC TGGAGTGGCA CCGCGCGAAC 
TCTGCCAACC TCAAGCTGCG CGAACTTTTT GAGGCGGATC CGGAGCGCTT CAACAACTTC 
AGCTTGAACC TCAACACCAA CCATGGGCAT ATTCTGGTGG ACTACTCCAA GAACCTTGTG 
AACAAGGAGG TGATGCAGAT GCTGGTGGAG CTGGCCAAGT CCAGAGGCGT GGAGGCTGCA 
CGGGACAACA TGTTCAGTGG TTCCAAGATC AACTACACCG AGAATCGGGC GGTGCTGCAT 
GTGGCCCTTC GGAACCGGTC CAACACACCC ATCAAGGTGG ACGGCAAAGA TGTGATGCCG 
GAGGTGAACA GGGTTCTGGA CAAGATGAAG TCTTTCTGCC AGCGGGTCCG GAGTGGTGAC 
TGGAAAGGGT ACACTGGCAA ATCCATCACG GACATCATCA ACATCGGCAT CGGGGGCTCT 
GACCTGGGAC CCCTCATGGT GACTGAAGCT CTCAAGCCTT ACTCGAAAGG AGGTCCCCGT 
GTCTGGTTTG TCTCTAACAT TGATGGGACC CACATTGCCA AAACACTGGC CAGCTTGTCC 
CCTGAGACTT CCCTCTTTAT AATCGCCTCC AAGACCTTCA CCACCCAGGA GACCATCACC 
AATGCAGAGA CAGCAAAGGA GTGGTTTCTC GAAGCGGCCA AGGATCCATC TGCAGTTGCA 
AAGCACTTTG TCGCCCTGTC TACGAACACG GCCAAAGTGA AAGAGTTTGG AATTGACCCT 
CAAAACATGT TCGAGTTCTG GGATTGGGTA GGTGGCCGCT ATTCGCTGTG GTCAGCCATT 
GGACTTTCCA TTGCTCTGCA TGTAGGTTTT GACCACTTCG AGCAGCTGCT GTCCGGGGCT 
CACTGGATGG ACCAGCACTT CCTCAAGACG CCCCTGGAGA AGAATGCCCC CGTCCTGCTG 
GCTCTACTGG GCATCTGGTA CATCAACTGC TACGGCTGTG AGACCCACGC CTTGCTGCCC 
TATGACCAGT ACATGCACCG CTTTGCTGCC TATTTCCAGC AGGGTGACAT GGAGTCCAAC 
GGAAAGTACA TCACCAAGTC CGGGGCCCGT GTGGACCACC AGACAGGCCC CATCGTGTGG 
GGGGAACCAG GGACCAATGG TCAACATGCA TTCTACCAGC TCATCCACCA AGGCACCAAG 
ATGATACCCT GTGACTTTCT CATCCCTGTC CAGACCCAGC ACCCCATACG GAAAGGTCTG 
CATCACAAGA TCCTCCTGGC TAACTTCTTG GCCCAGACTG AGGCCCTGAT GAAGGGGAAG 
TTGCCTGAAG AGGCCAGGAA GGAGCTCCAG GCTGCCGGAA AGAGCCCAGA AGACTTGGAG 
AAACTCTTGC CACACAAGGT CTTTGAAGGA AACCGCCCGA CCAACTCTAT TGTGTTTACC 
AAGCTGACAC CCTTCATTCT GGGGGCCTTG ATTGCCATGT ATGAGCACAA GATCTTTGTT 
CAGGGCATCA TGTGGGACAT CAACAGCTTC GACCAGTGGG GAGTGGAGCT GGGGAAGCAG 
CTGGCCAAGA AAATTGAGCC GGAGCTGGAG GGCAGCTCTG CTGTGACCTC CCATGATTCC 
TCCACTAACG GACTGATCAG CTTCATCAAG CAACAGCGGG ACACCAAACT AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032181.2
CDS	124..1800
Translation	MAALTRNPQFQKLLEWHRANSANLKLRELFEADPERFNNFSLNLNTNHGHILVDYSKNLVNKEVMQMLVELAKSRGVEAARDNMFSGSKINYTENRAVLHVALRNRSNTPIKVDGKDVMPEVNRVLDKMKSFCQRVRSGDWKGYTGKSITDIINIGIGGSDLGPLMVTEALKPYSKGGPRVWFVSNIDGTHIAKTLASLSPETSLFIIASKTFTTQETITNAETAKEWFLEAAKDPSAVAKHFVALSTNTAKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDHFEQLLSGAHWMDQHFLKTPLEKNAPVLLALLGIWYINCYGCETHALLPYDQYMHRFAAYFQQGDMESNGKYITKSGARVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMKGKLPEEARKELQAAGKSPEDLEKLLPHKVFEGNRPTNSIVFTKLTPFILGALIAMYEHKIFVQGIMWDINSFDQWGVELGKQLAKKIEPELEGSSAVTSHDSSTNGLISFIKQQRDTKLE

Target ORF information:

RefSeq Version	NM_008155.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus glucose-6-phosphate isomerase 1 (Gpi1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008155.4

ORF Insert Sequence:

ATGGCTGCGC TCACCCGGAA CCCGCAGTTC CAGAAGCTCC TGGAGTGGCA CCGCGCGAAC 
TCTGCCAACC TCAAGCTGCG CGAACTTTTT GAGGCGGATC CGGAGCGCTT CAACAACTTC 
AGCTTGAACC TCAACACCAA CCATGGGCAT ATTCTGGTGG ACTACTCCAA GAACCTTGTG 
AACAAGGAGG TGATGCAGAT GCTGGTGGAG CTGGCCAAGT CCAGAGGCGT GGAGGCTGCA 
CGGGACAACA TGTTCAGTGG TTCCAAGATC AACTACACCG AGAATCGGGC GGTGCTGCAT 
GTGGCCCTTC GGAACCGGTC CAACACACCC ATCAAGGTGG ACGGCAAAGA TGTGATGCCG 
GAGGTGAACA GGGTTCTGGA CAAGATGAAG TCTTTCTGCC AGCGGGTCCG GAGTGGTGAC 
TGGAAAGGGT ACACTGGCAA ATCCATCACG GACATCATCA ACATCGGCAT CGGGGGCTCT 
GACCTGGGAC CCCTCATGGT GACTGAAGCT CTCAAGCCTT ACTCGAAAGG AGGTCCCCGT 
GTCTGGTTTG TCTCTAACAT TGATGGGACC CACATTGCCA AAACACTGGC CAGCTTGTCC 
CCTGAGACTT CCCTCTTTAT AATCGCCTCC AAGACCTTCA CCACCCAGGA GACCATCACC 
AATGCAGAGA CAGCAAAGGA GTGGTTTCTC GAAGCGGCCA AGGATCCATC TGCAGTTGCA 
AAGCACTTTG TCGCCCTGTC TACGAACACG GCCAAAGTGA AAGAGTTTGG AATTGACCCT 
CAAAACATGT TCGAGTTCTG GGATTGGGTA GGTGGCCGCT ATTCGCTGTG GTCAGCCATT 
GGACTTTCCA TTGCTCTGCA TGTAGGTTTT GACCACTTCG AGCAGCTGCT GTCCGGGGCT 
CACTGGATGG ACCAGCACTT CCTCAAGACG CCCCTGGAGA AGAATGCCCC CGTCCTGCTG 
GCTCTACTGG GCATCTGGTA CATCAACTGC TACGGCTGTG AGACCCACGC CTTGCTGCCC 
TATGACCAGT ACATGCACCG CTTTGCTGCC TATTTCCAGC AGGGTGACAT GGAGTCCAAC 
GGAAAGTACA TCACCAAGTC CGGGGCCCGT GTGGACCACC AGACAGGCCC CATCGTGTGG 
GGGGAACCAG GGACCAATGG TCAACATGCA TTCTACCAGC TCATCCACCA AGGCACCAAG 
ATGATACCCT GTGACTTTCT CATCCCTGTC CAGACCCAGC ACCCCATACG GAAAGGTCTG 
CATCACAAGA TCCTCCTGGC TAACTTCTTG GCCCAGACTG AGGCCCTGAT GAAGGGGAAG 
TTGCCTGAAG AGGCCAGGAA GGAGCTCCAG GCTGCCGGAA AGAGCCCAGA AGACTTGGAG 
AAACTCTTGC CACACAAGGT CTTTGAAGGA AACCGCCCGA CCAACTCTAT TGTGTTTACC 
AAGCTGACAC CCTTCATTCT GGGGGCCTTG ATTGCCATGT ATGAGCACAA GATCTTTGTT 
CAGGGCATCA TGTGGGACAT CAACAGCTTC GACCAGTGGG GAGTGGAGCT GGGGAAGCAG 
CTGGCCAAGA AAATTGAGCC GGAGCTGGAG GGCAGCTCTG CTGTGACCTC CCATGATTCC 
TCCACTAACG GACTGATCAG CTTCATCAAG CAACAGCGGG ACACCAAACT AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hierarchically related lineage-restricted fates of multipotent haematopoietic stem cells.
Nature(2018 Jan)
Carrelha J,Meng Y,Kettyle LM,Luis TC,Norfo R,Alcolea V,Grasso F,Gambardella A,Grover A,H?gstrand K,Lord AM,Sanjuan-Pla A,Woll PS,Nerlov C,Sten Eirik W J

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

Tumor cell autocrine motility factor is the neuroleukin/phosphohexose isomerase polypeptide.
Cancer research56(13)2960-3(1996 Jul)
Watanabe H,Takehana K,Date M,Shinozaki T,Raz A

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Gpi1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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