Gpc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpc1
Entrez Gene ID	14733
Full Name	glypican 1
Synonyms	AI462976
General protein information	Preferred Names glypican 1 Names glypican-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_006529142.2	XP_006529205.1	glypican-1 isoform X2
XM_011247925.2	XP_011246227.1	glypican-1 isoform X1
NM_016696.5	NP_057905.1	glypican-1 precursor
XM_006529142.3	XP_006529205.1	glypican-1 isoform X2
NM_016696.4	NP_057905.1	glypican-1 precursor
XM_011247925.1	XP_011246227.1	glypican-1 isoform X1

Pathways from BioSystems

Homologies

	GPC1	XP_001089255.1
Xenopus tropicalis (tropical clawed frog)	gpc1	NP_001072316.1
Homo sapiens (human)	GPC1	NP_002072.2
Pan troglodytes (chimpanzee)	GPC1	XP_001156197.2
Danio rerio (zebrafish)	gpc1a	NP_001032450.1
Canis lupus familiaris (dog)	GPC1	XP_005636000.1
Gallus gallus (chicken)	GPC1	XP_422590.2
Danio rerio (zebrafish)	gpc1b	NP_957314.1
Bos taurus (cattle)	GPC1	NP_001039701.1
Mus musculus (house mouse)	Gpc1	NP_057905.1
Rattus norvegicus (Norway rat)	Gpc1	NP_110455.1

Gene Ontology
Bibliography

Related articles in PubMed

Alterations in mandibular morphology associated with glypican 1 and glypican 3 gene mutations.
Mian M, Ranjitkar S, Townsend GC, Anderson PJ
Orthodontics & craniofacial research20(3)183-187(2017 Aug)

Altered heparan sulfate structure in Glce(-/-) mice leads to increased Hedgehog signaling in endochondral bones.
Dierker T, Bachvarova V, Krause Y, Li JP, Kjell?n L, Seidler DG, Vortkamp A
Matrix biology : journal of the International Society for Matrix Biology4982-92(2016 Jan)

Novel insights into Notum and glypicans regulation in colorectal cancer.
De Robertis M, Arigoni M, Loiacono L, Riccardo F, Calogero RA, Feodorova Y, Tashkova D, Belovejdov V, Sarafian V, Cavallo F, Signori E
Oncotarget6(38)41237-57(2015 Dec)

Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.
Sharma P, Abbasi C, Lazic S, Teng AC, Wang D, Dubois N, Ignatchenko V, Wong V, Liu J, Araki T, Tiburcy M, Ackerley C, Zimmermann WH, Hamilton R, Sun Y, Liu PP, Keller G, Stagljar I, Scott IC, Kislinger T, Gramolini AO
Nature communications68391(2015 Sep)

PTP? functions as a presynaptic receptor for the glypican-4/LRRTM4 complex and is essential for excitatory synaptic transmission.
Ko JS, Pramanik G, Um JW, Shim JS, Lee D, Kim KH, Chung GY, Condomitti G, Kim HM, Kim H, de Wit J, Park KS, Tabuchi K, Ko J
Proceedings of the National Academy of Sciences of the United States of America112(6)1874-9(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis.
Title: Novel insights into Notum and glypicans regulation in colorectal cancer.

Amyloid precursor protein (APP)/APP-like protein 2 (APLP2) expression is required to initiate endosome-nucleus-autophagosome
Title: Amyloid precursor protein (APP)/APP-like protein 2 (APLP2) expression is required to initiate endosome-nucleus-autophagosome trafficking of glypican-1-derived heparan sulfate.

The N-terminal growth-factor-like domain of amyloid-beta precursor protein is necessary for protein-receptor binding, whereas the E2-domain mediates interaction with membrane-anchored syndecan 2 and glypican 1.
Title: Soluble amyloid-? precursor protein binds its cell surface receptor in a cooperative fashion with glypican and syndecan proteoglycans.

Glypican-1 is expressed by neural stem cells and neurons derived from embryonic stem cells, which are then surrounded with cell bodies and processes, which in certain cases show distinctive expression patterns.
Title: Glypican-1, phosphacan/receptor protein-tyrosine phosphatase-?/? and its ligand, tenascin-C, are expressed by neural stem cells and neural cells derived from embryonic stem cells.

Glypican-1 is a novel cellular cofactor for prion conversion. It acts as a scaffold facilitating the interaction of PrP(C) and PrP(Sc) in lipid rafts.
Title: Glypican-1 mediates both prion protein lipid raft association and disease isoform formation.

The following Gpc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21800	NM_016696.5 Latest version!	Mus musculus glypican 1 (Gpc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$328.30 $469.00
OMu27905	XM_006529142.3 Latest version!	Mus musculus glypican 1 (Gpc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu67840	XM_011247925.2 Latest version!	Mus musculus glypican 1 (Gpc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu27905	XM_006529142.2	Mus musculus glypican 1 (Gpc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu21800	NM_016696.4	Mus musculus glypican 1 (Gpc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$328.30 $469.00
OMu67840	XM_011247925.1	Mus musculus glypican 1 (Gpc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpc1 ( NM_016696.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_057905.1 Mus musculus glypican 1 (Gpc1), mRNA.

Price & Availability↑

CloneID	OMu21800
Clone ID Related Accession (Same CDS sequence)	NM_016696.5 , NM_016696.4
Accession Version	NM_016696.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	glypican-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131059.6. On Sep 19, 2017 this sequence version replaced NM_016696.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154899.1, AF185613.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTCC GGACCCGAGG CTGGTGGCTG CTGTGCGCGG CCGCCGCGCT GGTCGTCTGC 
GCCCGCGGGG ACCCCGCCAG CAAGAGCCGG AGCTGCAGCG AAGTCCGCCA GATCTACGGG 
GCTAAGGGCT TTAGCCTGAG CGATGTGCCT CAGGCAGAGA TCTCGGGTGA GCACCTGCGG 
ATCTGCCCCC AGGGCTACAC TTGCTGTACT AGTGAGATGG AGGAGAATTT GGCCAACCAC 
AGCCGAATGG AGCTGGAGAG CGCACTCCAT GACAGCAGCC GCGCCCTGCA GGCCACACTG 
GCCACCCAGC TGCATGGCAT CGATGACCAC TTCCAGCGCC TGCTGAATGA CTCGGAGCGC 
ACACTGCAGG AGGCTTTCCC TGGGGCCTTT GGGGACCTGT ATACGCAGAA CACTCGTGCC 
TTCCGGGACC TATATGCTGA GCTGCGCCTC TACTACCGTG GGGCCAACCT GCACCTTGAG 
GAGACGCTGG CCGAGTTCTG GGCACGGCTG CTGGAGCGCC TCTTCAAGCA GCTGCACCCC 
CAGCTGCTGC CTGATGACTA CCTGGACTGC CTGGGCAAGC AGGCGGAGGC ACTGCGGCCG 
TTTGGAGATG CCCCTCGAGA ACTGCGCCTG CGGGCCACCC GTGCCTTTGT GGCTGCACGT 
TCCTTTGTGC AGGGCCTGGG TGTGGCCAGT GATGTAGTCC GGAAGGTGGC CCAGGTACCT 
CTGGCCCCAG AATGTTCTCG GGCCATCATG AAGTTGGTCT ACTGTGCTCA TTGCCGGGGA 
GTCCCGGGCG CCCGGCCCTG CCCCGACTAT TGCCGAAATG TGCTCAAAGG CTGCCTTGCC 
AACCAGGCCG ACCTGGATGC CGAGTGGAGG AACCTCCTGG ACTCCATGGT GCTCATCACT 
GACAAGTTCT GGGGCCCGTC GGGTGCGGAG AGTGTCATTG GCGGTGTGCA CGTGTGGCTG 
GCGGAGGCCA TCAACGCCCT CCAGGACAAC AAGGACACAC TCACAGCTAA GGTCATCCAG 
GCCTGTGGAA ACCCCAAGGT CAATCCCCAC GGCTCTGGGC CCGAGGAGAA GCGTCGCCGT 
GGCAAATTGG CACTGCAGGA GAAGCCCTCC ACAGGTACTC TGGAAAAACT GGTCTCTGAG 
GCCAAGGCCC AGCTCCGAGA CATTCAGGAC TTCTGGATCA GCCTCCCAGG GACACTGTGC 
AGTGAGAAGA TGGCCATGAG TCCTGCCAGT GACGACCGCT GCTGGAATGG AATTTCCAAG 
GGCCGGTACC TACCAGAGGT GATGGGTGAC GGGCTGGCCA ACCAGATCAA CAACCCTGAG 
GTGGAAGTGG ACATCACCAA GCCAGACATG ACCATCCGCC AGCAGATTAT GCAGCTCAAG 
ATCATGACCA ACCGTTTACG TGGCGCCTAT GGCGGCAACG ACGTGGACTT CCAGGATGCT 
AGTGATGACG GCAGTGGCTC CGGCAGCGGT GGCGGATGCC CAGATGACAC CTGTGGCCGG 
AGGGTCAGCA AGAAGAGTTC CAGCTCCCGG ACCCCCTTGA CCCATGCCCT CCCCGGCCTG 
TCAGAACAGG AGGGACAGAA GACCTCAGCT GCCACCTGCC CAGAGCCCCA CAGCTTCTTC 
CTGCTCTTCC TCGTCACCTT GGTCCTTGCG GCAGCCAGGC CCAGGTGGCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057905.1
CDS	288..1961
Translation	MELRTRGWWLLCAAAALVVCARGDPASKSRSCSEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCTSEMEENLANHSRMELESALHDSSRALQATLATQLHGIDDHFQRLLNDSERTLQEAFPGAFGDLYTQNTRAFRDLYAELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLPDDYLDCLGKQAEALRPFGDAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLAPECSRAIMKLVYCAHCRGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGPSGAESVIGGVHVWLAEAINALQDNKDTLTAKVIQACGNPKVNPHGSGPEEKRRRGKLALQEKPSTGTLEKLVSEAKAQLRDIQDFWISLPGTLCSEKMAMSPASDDRCWNGISKGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRGAYGGNDVDFQDASDDGSGSGSGGGCPDDTCGRRVSKKSSSSRTPLTHALPGLSEQEGQKTSAATCPEPHSFFLLFLVTLVLAAARPRWR

Target ORF information:

RefSeq Version	NM_016696.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus glypican 1 (Gpc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016696.5

ORF Insert Sequence:

ATGGAACTCC GGACCCGAGG CTGGTGGCTG CTGTGCGCGG CCGCCGCGCT GGTCGTCTGC 
GCCCGCGGGG ACCCCGCCAG CAAGAGCCGG AGCTGCAGCG AAGTCCGCCA GATCTACGGG 
GCTAAGGGCT TTAGCCTGAG CGATGTGCCT CAGGCAGAGA TCTCGGGTGA GCACCTGCGG 
ATCTGCCCCC AGGGCTACAC TTGCTGTACT AGTGAGATGG AGGAGAATTT GGCCAACCAC 
AGCCGAATGG AGCTGGAGAG CGCACTCCAT GACAGCAGCC GCGCCCTGCA GGCCACACTG 
GCCACCCAGC TGCATGGCAT CGATGACCAC TTCCAGCGCC TGCTGAATGA CTCGGAGCGC 
ACACTGCAGG AGGCTTTCCC TGGGGCCTTT GGGGACCTGT ATACGCAGAA CACTCGTGCC 
TTCCGGGACC TATATGCTGA GCTGCGCCTC TACTACCGTG GGGCCAACCT GCACCTTGAG 
GAGACGCTGG CCGAGTTCTG GGCACGGCTG CTGGAGCGCC TCTTCAAGCA GCTGCACCCC 
CAGCTGCTGC CTGATGACTA CCTGGACTGC CTGGGCAAGC AGGCGGAGGC ACTGCGGCCG 
TTTGGAGATG CCCCTCGAGA ACTGCGCCTG CGGGCCACCC GTGCCTTTGT GGCTGCACGT 
TCCTTTGTGC AGGGCCTGGG TGTGGCCAGT GATGTAGTCC GGAAGGTGGC CCAGGTACCT 
CTGGCCCCAG AATGTTCTCG GGCCATCATG AAGTTGGTCT ACTGTGCTCA TTGCCGGGGA 
GTCCCGGGCG CCCGGCCCTG CCCCGACTAT TGCCGAAATG TGCTCAAAGG CTGCCTTGCC 
AACCAGGCCG ACCTGGATGC CGAGTGGAGG AACCTCCTGG ACTCCATGGT GCTCATCACT 
GACAAGTTCT GGGGCCCGTC GGGTGCGGAG AGTGTCATTG GCGGTGTGCA CGTGTGGCTG 
GCGGAGGCCA TCAACGCCCT CCAGGACAAC AAGGACACAC TCACAGCTAA GGTCATCCAG 
GCCTGTGGAA ACCCCAAGGT CAATCCCCAC GGCTCTGGGC CCGAGGAGAA GCGTCGCCGT 
GGCAAATTGG CACTGCAGGA GAAGCCCTCC ACAGGTACTC TGGAAAAACT GGTCTCTGAG 
GCCAAGGCCC AGCTCCGAGA CATTCAGGAC TTCTGGATCA GCCTCCCAGG GACACTGTGC 
AGTGAGAAGA TGGCCATGAG TCCTGCCAGT GACGACCGCT GCTGGAATGG AATTTCCAAG 
GGCCGGTACC TACCAGAGGT GATGGGTGAC GGGCTGGCCA ACCAGATCAA CAACCCTGAG 
GTGGAAGTGG ACATCACCAA GCCAGACATG ACCATCCGCC AGCAGATTAT GCAGCTCAAG 
ATCATGACCA ACCGTTTACG TGGCGCCTAT GGCGGCAACG ACGTGGACTT CCAGGATGCT 
AGTGATGACG GCAGTGGCTC CGGCAGCGGT GGCGGATGCC CAGATGACAC CTGTGGCCGG 
AGGGTCAGCA AGAAGAGTTC CAGCTCCCGG ACCCCCTTGA CCCATGCCCT CCCCGGCCTG 
TCAGAACAGG AGGGACAGAA GACCTCAGCT GCCACCTGCC CAGAGCCCCA CAGCTTCTTC 
CTGCTCTTCC TCGTCACCTT GGTCCTTGCG GCAGCCAGGC CCAGGTGGCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpc1 ( NM_016696.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_057905.1 Mus musculus glypican 1 (Gpc1), mRNA.

Price & Availability↑

CloneID	OMu21800
Clone ID Related Accession (Same CDS sequence)	NM_016696.5 , NM_016696.4
Accession Version	NM_016696.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-12
Organism	Mus musculus(House mouse)
Product	glypican-1 precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC131059.6. On Oct 19, 2007 this sequence version replaced NM_016696.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF185613.1, AK154899.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAACTCC GGACCCGAGG CTGGTGGCTG CTGTGCGCGG CCGCCGCGCT GGTCGTCTGC 
GCCCGCGGGG ACCCCGCCAG CAAGAGCCGG AGCTGCAGCG AAGTCCGCCA GATCTACGGG 
GCTAAGGGCT TTAGCCTGAG CGATGTGCCT CAGGCAGAGA TCTCGGGTGA GCACCTGCGG 
ATCTGCCCCC AGGGCTACAC TTGCTGTACT AGTGAGATGG AGGAGAATTT GGCCAACCAC 
AGCCGAATGG AGCTGGAGAG CGCACTCCAT GACAGCAGCC GCGCCCTGCA GGCCACACTG 
GCCACCCAGC TGCATGGCAT CGATGACCAC TTCCAGCGCC TGCTGAATGA CTCGGAGCGC 
ACACTGCAGG AGGCTTTCCC TGGGGCCTTT GGGGACCTGT ATACGCAGAA CACTCGTGCC 
TTCCGGGACC TATATGCTGA GCTGCGCCTC TACTACCGTG GGGCCAACCT GCACCTTGAG 
GAGACGCTGG CCGAGTTCTG GGCACGGCTG CTGGAGCGCC TCTTCAAGCA GCTGCACCCC 
CAGCTGCTGC CTGATGACTA CCTGGACTGC CTGGGCAAGC AGGCGGAGGC ACTGCGGCCG 
TTTGGAGATG CCCCTCGAGA ACTGCGCCTG CGGGCCACCC GTGCCTTTGT GGCTGCACGT 
TCCTTTGTGC AGGGCCTGGG TGTGGCCAGT GATGTAGTCC GGAAGGTGGC CCAGGTACCT 
CTGGCCCCAG AATGTTCTCG GGCCATCATG AAGTTGGTCT ACTGTGCTCA TTGCCGGGGA 
GTCCCGGGCG CCCGGCCCTG CCCCGACTAT TGCCGAAATG TGCTCAAAGG CTGCCTTGCC 
AACCAGGCCG ACCTGGATGC CGAGTGGAGG AACCTCCTGG ACTCCATGGT GCTCATCACT 
GACAAGTTCT GGGGCCCGTC GGGTGCGGAG AGTGTCATTG GCGGTGTGCA CGTGTGGCTG 
GCGGAGGCCA TCAACGCCCT CCAGGACAAC AAGGACACAC TCACAGCTAA GGTCATCCAG 
GCCTGTGGAA ACCCCAAGGT CAATCCCCAC GGCTCTGGGC CCGAGGAGAA GCGTCGCCGT 
GGCAAATTGG CACTGCAGGA GAAGCCCTCC ACAGGTACTC TGGAAAAACT GGTCTCTGAG 
GCCAAGGCCC AGCTCCGAGA CATTCAGGAC TTCTGGATCA GCCTCCCAGG GACACTGTGC 
AGTGAGAAGA TGGCCATGAG TCCTGCCAGT GACGACCGCT GCTGGAATGG AATTTCCAAG 
GGCCGGTACC TACCAGAGGT GATGGGTGAC GGGCTGGCCA ACCAGATCAA CAACCCTGAG 
GTGGAAGTGG ACATCACCAA GCCAGACATG ACCATCCGCC AGCAGATTAT GCAGCTCAAG 
ATCATGACCA ACCGTTTACG TGGCGCCTAT GGCGGCAACG ACGTGGACTT CCAGGATGCT 
AGTGATGACG GCAGTGGCTC CGGCAGCGGT GGCGGATGCC CAGATGACAC CTGTGGCCGG 
AGGGTCAGCA AGAAGAGTTC CAGCTCCCGG ACCCCCTTGA CCCATGCCCT CCCCGGCCTG 
TCAGAACAGG AGGGACAGAA GACCTCAGCT GCCACCTGCC CAGAGCCCCA CAGCTTCTTC 
CTGCTCTTCC TCGTCACCTT GGTCCTTGCG GCAGCCAGGC CCAGGTGGCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057905.1
CDS	247..1920
Translation	MELRTRGWWLLCAAAALVVCARGDPASKSRSCSEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCTSEMEENLANHSRMELESALHDSSRALQATLATQLHGIDDHFQRLLNDSERTLQEAFPGAFGDLYTQNTRAFRDLYAELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLPDDYLDCLGKQAEALRPFGDAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLAPECSRAIMKLVYCAHCRGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGPSGAESVIGGVHVWLAEAINALQDNKDTLTAKVIQACGNPKVNPHGSGPEEKRRRGKLALQEKPSTGTLEKLVSEAKAQLRDIQDFWISLPGTLCSEKMAMSPASDDRCWNGISKGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRGAYGGNDVDFQDASDDGSGSGSGGGCPDDTCGRRVSKKSSSSRTPLTHALPGLSEQEGQKTSAATCPEPHSFFLLFLVTLVLAAARPRWR

Target ORF information:

RefSeq Version	NM_016696.4
Organism	Mus musculus(House mouse)
Definition	Mus musculus glypican 1 (Gpc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016696.4

ORF Insert Sequence:

ATGGAACTCC GGACCCGAGG CTGGTGGCTG CTGTGCGCGG CCGCCGCGCT GGTCGTCTGC 
GCCCGCGGGG ACCCCGCCAG CAAGAGCCGG AGCTGCAGCG AAGTCCGCCA GATCTACGGG 
GCTAAGGGCT TTAGCCTGAG CGATGTGCCT CAGGCAGAGA TCTCGGGTGA GCACCTGCGG 
ATCTGCCCCC AGGGCTACAC TTGCTGTACT AGTGAGATGG AGGAGAATTT GGCCAACCAC 
AGCCGAATGG AGCTGGAGAG CGCACTCCAT GACAGCAGCC GCGCCCTGCA GGCCACACTG 
GCCACCCAGC TGCATGGCAT CGATGACCAC TTCCAGCGCC TGCTGAATGA CTCGGAGCGC 
ACACTGCAGG AGGCTTTCCC TGGGGCCTTT GGGGACCTGT ATACGCAGAA CACTCGTGCC 
TTCCGGGACC TATATGCTGA GCTGCGCCTC TACTACCGTG GGGCCAACCT GCACCTTGAG 
GAGACGCTGG CCGAGTTCTG GGCACGGCTG CTGGAGCGCC TCTTCAAGCA GCTGCACCCC 
CAGCTGCTGC CTGATGACTA CCTGGACTGC CTGGGCAAGC AGGCGGAGGC ACTGCGGCCG 
TTTGGAGATG CCCCTCGAGA ACTGCGCCTG CGGGCCACCC GTGCCTTTGT GGCTGCACGT 
TCCTTTGTGC AGGGCCTGGG TGTGGCCAGT GATGTAGTCC GGAAGGTGGC CCAGGTACCT 
CTGGCCCCAG AATGTTCTCG GGCCATCATG AAGTTGGTCT ACTGTGCTCA TTGCCGGGGA 
GTCCCGGGCG CCCGGCCCTG CCCCGACTAT TGCCGAAATG TGCTCAAAGG CTGCCTTGCC 
AACCAGGCCG ACCTGGATGC CGAGTGGAGG AACCTCCTGG ACTCCATGGT GCTCATCACT 
GACAAGTTCT GGGGCCCGTC GGGTGCGGAG AGTGTCATTG GCGGTGTGCA CGTGTGGCTG 
GCGGAGGCCA TCAACGCCCT CCAGGACAAC AAGGACACAC TCACAGCTAA GGTCATCCAG 
GCCTGTGGAA ACCCCAAGGT CAATCCCCAC GGCTCTGGGC CCGAGGAGAA GCGTCGCCGT 
GGCAAATTGG CACTGCAGGA GAAGCCCTCC ACAGGTACTC TGGAAAAACT GGTCTCTGAG 
GCCAAGGCCC AGCTCCGAGA CATTCAGGAC TTCTGGATCA GCCTCCCAGG GACACTGTGC 
AGTGAGAAGA TGGCCATGAG TCCTGCCAGT GACGACCGCT GCTGGAATGG AATTTCCAAG 
GGCCGGTACC TACCAGAGGT GATGGGTGAC GGGCTGGCCA ACCAGATCAA CAACCCTGAG 
GTGGAAGTGG ACATCACCAA GCCAGACATG ACCATCCGCC AGCAGATTAT GCAGCTCAAG 
ATCATGACCA ACCGTTTACG TGGCGCCTAT GGCGGCAACG ACGTGGACTT CCAGGATGCT 
AGTGATGACG GCAGTGGCTC CGGCAGCGGT GGCGGATGCC CAGATGACAC CTGTGGCCGG 
AGGGTCAGCA AGAAGAGTTC CAGCTCCCGG ACCCCCTTGA CCCATGCCCT CCCCGGCCTG 
TCAGAACAGG AGGGACAGAA GACCTCAGCT GCCACCTGCC CAGAGCCCCA CAGCTTCTTC 
CTGCTCTTCC TCGTCACCTT GGTCCTTGCG GCAGCCAGGC CCAGGTGGCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpc1 ( XM_006529142.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529205.1 Mus musculus glypican 1 (Gpc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu27905
Clone ID Related Accession (Same CDS sequence)	XM_006529142.2 , XM_006529142.3
Accession Version	XM_006529142.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glypican-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ATTTGGCCAA CCACAGCCGA ATGGAGCTGG AGAGCGCACT CCATGACAGC 
AGCCGCGCCC TGCAGGCCAC ACTGGCCACC CAGCTGCATG GCATCGATGA CCACTTCCAG 
CGCCTGCTGA ATGACTCGGA GCGCACACTG CAGGAGGCTT TCCCTGGGGC CTTTGGGGAC 
CTGTATACGC AGAACACTCG TGCCTTCCGG GACCTATATG CTGAGCTGCG CCTCTACTAC 
CGTGGGGCCA ACCTGCACCT TGAGGAGACG CTGGCCGAGT TCTGGGCACG GCTGCTGGAG 
CGCCTCTTCA AGCAGCTGCA CCCCCAGCTG CTGCCTGATG ACTACCTGGA CTGCCTGGGC 
AAGCAGGCGG AGGCACTGCG GCCGTTTGGA GATGCCCCTC GAGAACTGCG CCTGCGGGCC 
ACCCGTGCCT TTGTGGCTGC ACGTTCCTTT GTGCAGGGCC TGGGTGTGGC CAGTGATGTA 
GTCCGGAAGG TGGCCCAGGT ACCTCTGGCC CCAGAATGTT CTCGGGCCAT CATGAAGTTG 
GTCTACTGTG CTCATTGCCG GGGAGTCCCG GGCGCCCGGC CCTGCCCCGA CTATTGCCGA 
AATGTGCTCA AAGGCTGCCT TGCCAACCAG GCCGACCTGG ATGCCGAGTG GAGGAACCTC 
CTGGACTCCA TGGTGCTCAT CACTGACAAG TTCTGGGGCC CGTCGGGTGC GGAGAGTGTC 
ATTGGCGGTG TGCACGTGTG GCTGGCGGAG GCCATCAACG CCCTCCAGGA CAACAAGGAC 
ACACTCACAG CTAAGGTCAT CCAGGCCTGT GGAAACCCCA AGGTCAATCC CCACGGCTCT 
GGGCCCGAGG AGAAGCGTCG CCGTGGCAAA TTGGCACTGC AGGAGAAGCC CTCCACAGGT 
ACTCTGGAAA AACTGGTCTC TGAGGCCAAG GCCCAGCTCC GAGACATTCA GGACTTCTGG 
ATCAGCCTCC CAGGGACACT GTGCAGTGAG AAGATGGCCA TGAGTCCTGC CAGTGACGAC 
CGCTGCTGGA ATGGAATTTC CAAGGGCCGG TACCTACCAG AGGTGATGGG TGACGGGCTG 
GCCAACCAGA TCAACAACCC TGAGGTGGAA GTGGACATCA CCAAGCCAGA CATGACCATC 
CGCCAGCAGA TTATGCAGCT CAAGATCATG ACCAACCGTT TACGTGGCGC CTATGGCGGC 
AACGACGTGG ACTTCCAGGA TGCTAGTGAT GACGGCAGTG GCTCCGGCAG CGGTGGCGGA 
TGCCCAGATG ACACCTGTGG CCGGAGGGTC AGCAAGAAGA GTTCCAGCTC CCGGACCCCC 
TTGACCCATG CCCTCCCCGG CCTGTCAGAA CAGGAGGGAC AGAAGACCTC AGCTGCCACC 
TGCCCAGAGC CCCACAGCTT CTTCCTGCTC TTCCTCGTCA CCTTGGTCCT TGCGGCAGCC 
AGGCCCAGGT GGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529205.1
CDS	1718..3175
Translation	MEENLANHSRMELESALHDSSRALQATLATQLHGIDDHFQRLLNDSERTLQEAFPGAFGDLYTQNTRAFRDLYAELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLPDDYLDCLGKQAEALRPFGDAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLAPECSRAIMKLVYCAHCRGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGPSGAESVIGGVHVWLAEAINALQDNKDTLTAKVIQACGNPKVNPHGSGPEEKRRRGKLALQEKPSTGTLEKLVSEAKAQLRDIQDFWISLPGTLCSEKMAMSPASDDRCWNGISKGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRGAYGGNDVDFQDASDDGSGSGSGGGCPDDTCGRRVSKKSSSSRTPLTHALPGLSEQEGQKTSAATCPEPHSFFLLFLVTLVLAAARPRWR

Target ORF information:

RefSeq Version	XM_006529142.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glypican 1 (Gpc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529142.2

ORF Insert Sequence:

ATGGAGGAGA ATTTGGCCAA CCACAGCCGA ATGGAGCTGG AGAGCGCACT CCATGACAGC 
AGCCGCGCCC TGCAGGCCAC ACTGGCCACC CAGCTGCATG GCATCGATGA CCACTTCCAG 
CGCCTGCTGA ATGACTCGGA GCGCACACTG CAGGAGGCTT TCCCTGGGGC CTTTGGGGAC 
CTGTATACGC AGAACACTCG TGCCTTCCGG GACCTATATG CTGAGCTGCG CCTCTACTAC 
CGTGGGGCCA ACCTGCACCT TGAGGAGACG CTGGCCGAGT TCTGGGCACG GCTGCTGGAG 
CGCCTCTTCA AGCAGCTGCA CCCCCAGCTG CTGCCTGATG ACTACCTGGA CTGCCTGGGC 
AAGCAGGCGG AGGCACTGCG GCCGTTTGGA GATGCCCCTC GAGAACTGCG CCTGCGGGCC 
ACCCGTGCCT TTGTGGCTGC ACGTTCCTTT GTGCAGGGCC TGGGTGTGGC CAGTGATGTA 
GTCCGGAAGG TGGCCCAGGT ACCTCTGGCC CCAGAATGTT CTCGGGCCAT CATGAAGTTG 
GTCTACTGTG CTCATTGCCG GGGAGTCCCG GGCGCCCGGC CCTGCCCCGA CTATTGCCGA 
AATGTGCTCA AAGGCTGCCT TGCCAACCAG GCCGACCTGG ATGCCGAGTG GAGGAACCTC 
CTGGACTCCA TGGTGCTCAT CACTGACAAG TTCTGGGGCC CGTCGGGTGC GGAGAGTGTC 
ATTGGCGGTG TGCACGTGTG GCTGGCGGAG GCCATCAACG CCCTCCAGGA CAACAAGGAC 
ACACTCACAG CTAAGGTCAT CCAGGCCTGT GGAAACCCCA AGGTCAATCC CCACGGCTCT 
GGGCCCGAGG AGAAGCGTCG CCGTGGCAAA TTGGCACTGC AGGAGAAGCC CTCCACAGGT 
ACTCTGGAAA AACTGGTCTC TGAGGCCAAG GCCCAGCTCC GAGACATTCA GGACTTCTGG 
ATCAGCCTCC CAGGGACACT GTGCAGTGAG AAGATGGCCA TGAGTCCTGC CAGTGACGAC 
CGCTGCTGGA ATGGAATTTC CAAGGGCCGG TACCTACCAG AGGTGATGGG TGACGGGCTG 
GCCAACCAGA TCAACAACCC TGAGGTGGAA GTGGACATCA CCAAGCCAGA CATGACCATC 
CGCCAGCAGA TTATGCAGCT CAAGATCATG ACCAACCGTT TACGTGGCGC CTATGGCGGC 
AACGACGTGG ACTTCCAGGA TGCTAGTGAT GACGGCAGTG GCTCCGGCAG CGGTGGCGGA 
TGCCCAGATG ACACCTGTGG CCGGAGGGTC AGCAAGAAGA GTTCCAGCTC CCGGACCCCC 
TTGACCCATG CCCTCCCCGG CCTGTCAGAA CAGGAGGGAC AGAAGACCTC AGCTGCCACC 
TGCCCAGAGC CCCACAGCTT CTTCCTGCTC TTCCTCGTCA CCTTGGTCCT TGCGGCAGCC 
AGGCCCAGGT GGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpc1 ( XM_011247925.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246227.1 Mus musculus glypican 1 (Gpc1), transcript variant X1, mRNA.

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CloneID	OMu67840
Clone ID Related Accession (Same CDS sequence)	XM_011247925.2 , XM_011247925.1
Accession Version	XM_011247925.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glypican-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGC ACCTGCGGAT CTGCCCCCAG GGCTACACTT GCTGTACTAG TGAGATGGAG 
GAGAATTTGG CCAACCACAG CCGAATGGAG CTGGAGAGCG CACTCCATGA CAGCAGCCGC 
GCCCTGCAGG CCACACTGGC CACCCAGCTG CATGGCATCG ATGACCACTT CCAGCGCCTG 
CTGAATGACT CGGAGCGCAC ACTGCAGGAG GCTTTCCCTG GGGCCTTTGG GGACCTGTAT 
ACGCAGAACA CTCGTGCCTT CCGGGACCTA TATGCTGAGC TGCGCCTCTA CTACCGTGGG 
GCCAACCTGC ACCTTGAGGA GACGCTGGCC GAGTTCTGGG CACGGCTGCT GGAGCGCCTC 
TTCAAGCAGC TGCACCCCCA GCTGCTGCCT GATGACTACC TGGACTGCCT GGGCAAGCAG 
GCGGAGGCAC TGCGGCCGTT TGGAGATGCC CCTCGAGAAC TGCGCCTGCG GGCCACCCGT 
GCCTTTGTGG CTGCACGTTC CTTTGTGCAG GGCCTGGGTG TGGCCAGTGA TGTAGTCCGG 
AAGGTGGCCC AGGTACCTCT GGCCCCAGAA TGTTCTCGGG CCATCATGAA GTTGGTCTAC 
TGTGCTCATT GCCGGGGAGT CCCGGGCGCC CGGCCCTGCC CCGACTATTG CCGAAATGTG 
CTCAAAGGCT GCCTTGCCAA CCAGGCCGAC CTGGATGCCG AGTGGAGGAA CCTCCTGGAC 
TCCATGGTGC TCATCACTGA CAAGTTCTGG GGCCCGTCGG GTGCGGAGAG TGTCATTGGC 
GGTGTGCACG TGTGGCTGGC GGAGGCCATC AACGCCCTCC AGGACAACAA GGACACACTC 
ACAGCTAAGG TCATCCAGGC CTGTGGAAAC CCCAAGGTCA ATCCCCACGG CTCTGGGCCC 
GAGGAGAAGC GTCGCCGTGG CAAATTGGCA CTGCAGGAGA AGCCCTCCAC AGGTACTCTG 
GAAAAACTGG TCTCTGAGGC CAAGGCCCAG CTCCGAGACA TTCAGGACTT CTGGATCAGC 
CTCCCAGGGA CACTGTGCAG TGAGAAGATG GCCATGAGTC CTGCCAGTGA CGACCGCTGC 
TGGAATGGAA TTTCCAAGGG CCGGTACCTA CCAGAGGTGA TGGGTGACGG GCTGGCCAAC 
CAGATCAACA ACCCTGAGGT GGAAGTGGAC ATCACCAAGC CAGACATGAC CATCCGCCAG 
CAGATTATGC AGCTCAAGAT CATGACCAAC CGTTTACGTG GCGCCTATGG CGGCAACGAC 
GTGGACTTCC AGGATGCTAG TGATGACGGC AGTGGCTCCG GCAGCGGTGG CGGATGCCCA 
GATGACACCT GTGGCCGGAG GGTCAGCAAG AAGAGTTCCA GCTCCCGGAC CCCCTTGACC 
CATGCCCTCC CCGGCCTGTC AGAACAGGAG GGACAGAAGA CCTCAGCTGC CACCTGCCCA 
GAGCCCCACA GCTTCTTCCT GCTCTTCCTC GTCACCTTGG TCCTTGCGGC AGCCAGGCCC 
AGGTGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246227.1
CDS	119..1630
Translation	MGEHLRICPQGYTCCTSEMEENLANHSRMELESALHDSSRALQATLATQLHGIDDHFQRLLNDSERTLQEAFPGAFGDLYTQNTRAFRDLYAELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLPDDYLDCLGKQAEALRPFGDAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLAPECSRAIMKLVYCAHCRGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGPSGAESVIGGVHVWLAEAINALQDNKDTLTAKVIQACGNPKVNPHGSGPEEKRRRGKLALQEKPSTGTLEKLVSEAKAQLRDIQDFWISLPGTLCSEKMAMSPASDDRCWNGISKGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRGAYGGNDVDFQDASDDGSGSGSGGGCPDDTCGRRVSKKSSSSRTPLTHALPGLSEQEGQKTSAATCPEPHSFFLLFLVTLVLAAARPRWR

Target ORF information:

RefSeq Version	XM_011247925.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus glypican 1 (Gpc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247925.1

ORF Insert Sequence:

ATGGGTGAGC ACCTGCGGAT CTGCCCCCAG GGCTACACTT GCTGTACTAG TGAGATGGAG 
GAGAATTTGG CCAACCACAG CCGAATGGAG CTGGAGAGCG CACTCCATGA CAGCAGCCGC 
GCCCTGCAGG CCACACTGGC CACCCAGCTG CATGGCATCG ATGACCACTT CCAGCGCCTG 
CTGAATGACT CGGAGCGCAC ACTGCAGGAG GCTTTCCCTG GGGCCTTTGG GGACCTGTAT 
ACGCAGAACA CTCGTGCCTT CCGGGACCTA TATGCTGAGC TGCGCCTCTA CTACCGTGGG 
GCCAACCTGC ACCTTGAGGA GACGCTGGCC GAGTTCTGGG CACGGCTGCT GGAGCGCCTC 
TTCAAGCAGC TGCACCCCCA GCTGCTGCCT GATGACTACC TGGACTGCCT GGGCAAGCAG 
GCGGAGGCAC TGCGGCCGTT TGGAGATGCC CCTCGAGAAC TGCGCCTGCG GGCCACCCGT 
GCCTTTGTGG CTGCACGTTC CTTTGTGCAG GGCCTGGGTG TGGCCAGTGA TGTAGTCCGG 
AAGGTGGCCC AGGTACCTCT GGCCCCAGAA TGTTCTCGGG CCATCATGAA GTTGGTCTAC 
TGTGCTCATT GCCGGGGAGT CCCGGGCGCC CGGCCCTGCC CCGACTATTG CCGAAATGTG 
CTCAAAGGCT GCCTTGCCAA CCAGGCCGAC CTGGATGCCG AGTGGAGGAA CCTCCTGGAC 
TCCATGGTGC TCATCACTGA CAAGTTCTGG GGCCCGTCGG GTGCGGAGAG TGTCATTGGC 
GGTGTGCACG TGTGGCTGGC GGAGGCCATC AACGCCCTCC AGGACAACAA GGACACACTC 
ACAGCTAAGG TCATCCAGGC CTGTGGAAAC CCCAAGGTCA ATCCCCACGG CTCTGGGCCC 
GAGGAGAAGC GTCGCCGTGG CAAATTGGCA CTGCAGGAGA AGCCCTCCAC AGGTACTCTG 
GAAAAACTGG TCTCTGAGGC CAAGGCCCAG CTCCGAGACA TTCAGGACTT CTGGATCAGC 
CTCCCAGGGA CACTGTGCAG TGAGAAGATG GCCATGAGTC CTGCCAGTGA CGACCGCTGC 
TGGAATGGAA TTTCCAAGGG CCGGTACCTA CCAGAGGTGA TGGGTGACGG GCTGGCCAAC 
CAGATCAACA ACCCTGAGGT GGAAGTGGAC ATCACCAAGC CAGACATGAC CATCCGCCAG 
CAGATTATGC AGCTCAAGAT CATGACCAAC CGTTTACGTG GCGCCTATGG CGGCAACGAC 
GTGGACTTCC AGGATGCTAG TGATGACGGC AGTGGCTCCG GCAGCGGTGG CGGATGCCCA 
GATGACACCT GTGGCCGGAG GGTCAGCAAG AAGAGTTCCA GCTCCCGGAC CCCCTTGACC 
CATGCCCTCC CCGGCCTGTC AGAACAGGAG GGACAGAAGA CCTCAGCTGC CACCTGCCCA 
GAGCCCCACA GCTTCTTCCT GCTCTTCCTC GTCACCTTGG TCCTTGCGGC AGCCAGGCCC 
AGGTGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpc1 ( XM_006529142.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529205.1 Mus musculus glypican 1 (Gpc1), transcript variant X2, mRNA.

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CloneID	OMu27905
Clone ID Related Accession (Same CDS sequence)	XM_006529142.2 , XM_006529142.3
Accession Version	XM_006529142.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glypican-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ATTTGGCCAA CCACAGCCGA ATGGAGCTGG AGAGCGCACT CCATGACAGC 
AGCCGCGCCC TGCAGGCCAC ACTGGCCACC CAGCTGCATG GCATCGATGA CCACTTCCAG 
CGCCTGCTGA ATGACTCGGA GCGCACACTG CAGGAGGCTT TCCCTGGGGC CTTTGGGGAC 
CTGTATACGC AGAACACTCG TGCCTTCCGG GACCTATATG CTGAGCTGCG CCTCTACTAC 
CGTGGGGCCA ACCTGCACCT TGAGGAGACG CTGGCCGAGT TCTGGGCACG GCTGCTGGAG 
CGCCTCTTCA AGCAGCTGCA CCCCCAGCTG CTGCCTGATG ACTACCTGGA CTGCCTGGGC 
AAGCAGGCGG AGGCACTGCG GCCGTTTGGA GATGCCCCTC GAGAACTGCG CCTGCGGGCC 
ACCCGTGCCT TTGTGGCTGC ACGTTCCTTT GTGCAGGGCC TGGGTGTGGC CAGTGATGTA 
GTCCGGAAGG TGGCCCAGGT ACCTCTGGCC CCAGAATGTT CTCGGGCCAT CATGAAGTTG 
GTCTACTGTG CTCATTGCCG GGGAGTCCCG GGCGCCCGGC CCTGCCCCGA CTATTGCCGA 
AATGTGCTCA AAGGCTGCCT TGCCAACCAG GCCGACCTGG ATGCCGAGTG GAGGAACCTC 
CTGGACTCCA TGGTGCTCAT CACTGACAAG TTCTGGGGCC CGTCGGGTGC GGAGAGTGTC 
ATTGGCGGTG TGCACGTGTG GCTGGCGGAG GCCATCAACG CCCTCCAGGA CAACAAGGAC 
ACACTCACAG CTAAGGTCAT CCAGGCCTGT GGAAACCCCA AGGTCAATCC CCACGGCTCT 
GGGCCCGAGG AGAAGCGTCG CCGTGGCAAA TTGGCACTGC AGGAGAAGCC CTCCACAGGT 
ACTCTGGAAA AACTGGTCTC TGAGGCCAAG GCCCAGCTCC GAGACATTCA GGACTTCTGG 
ATCAGCCTCC CAGGGACACT GTGCAGTGAG AAGATGGCCA TGAGTCCTGC CAGTGACGAC 
CGCTGCTGGA ATGGAATTTC CAAGGGCCGG TACCTACCAG AGGTGATGGG TGACGGGCTG 
GCCAACCAGA TCAACAACCC TGAGGTGGAA GTGGACATCA CCAAGCCAGA CATGACCATC 
CGCCAGCAGA TTATGCAGCT CAAGATCATG ACCAACCGTT TACGTGGCGC CTATGGCGGC 
AACGACGTGG ACTTCCAGGA TGCTAGTGAT GACGGCAGTG GCTCCGGCAG CGGTGGCGGA 
TGCCCAGATG ACACCTGTGG CCGGAGGGTC AGCAAGAAGA GTTCCAGCTC CCGGACCCCC 
TTGACCCATG CCCTCCCCGG CCTGTCAGAA CAGGAGGGAC AGAAGACCTC AGCTGCCACC 
TGCCCAGAGC CCCACAGCTT CTTCCTGCTC TTCCTCGTCA CCTTGGTCCT TGCGGCAGCC 
AGGCCCAGGT GGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529205.1
CDS	1721..3178
Translation	MEENLANHSRMELESALHDSSRALQATLATQLHGIDDHFQRLLNDSERTLQEAFPGAFGDLYTQNTRAFRDLYAELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLPDDYLDCLGKQAEALRPFGDAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLAPECSRAIMKLVYCAHCRGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGPSGAESVIGGVHVWLAEAINALQDNKDTLTAKVIQACGNPKVNPHGSGPEEKRRRGKLALQEKPSTGTLEKLVSEAKAQLRDIQDFWISLPGTLCSEKMAMSPASDDRCWNGISKGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRGAYGGNDVDFQDASDDGSGSGSGGGCPDDTCGRRVSKKSSSSRTPLTHALPGLSEQEGQKTSAATCPEPHSFFLLFLVTLVLAAARPRWR

Target ORF information:

RefSeq Version	XM_006529142.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glypican 1 (Gpc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529142.3

ORF Insert Sequence:

ATGGAGGAGA ATTTGGCCAA CCACAGCCGA ATGGAGCTGG AGAGCGCACT CCATGACAGC 
AGCCGCGCCC TGCAGGCCAC ACTGGCCACC CAGCTGCATG GCATCGATGA CCACTTCCAG 
CGCCTGCTGA ATGACTCGGA GCGCACACTG CAGGAGGCTT TCCCTGGGGC CTTTGGGGAC 
CTGTATACGC AGAACACTCG TGCCTTCCGG GACCTATATG CTGAGCTGCG CCTCTACTAC 
CGTGGGGCCA ACCTGCACCT TGAGGAGACG CTGGCCGAGT TCTGGGCACG GCTGCTGGAG 
CGCCTCTTCA AGCAGCTGCA CCCCCAGCTG CTGCCTGATG ACTACCTGGA CTGCCTGGGC 
AAGCAGGCGG AGGCACTGCG GCCGTTTGGA GATGCCCCTC GAGAACTGCG CCTGCGGGCC 
ACCCGTGCCT TTGTGGCTGC ACGTTCCTTT GTGCAGGGCC TGGGTGTGGC CAGTGATGTA 
GTCCGGAAGG TGGCCCAGGT ACCTCTGGCC CCAGAATGTT CTCGGGCCAT CATGAAGTTG 
GTCTACTGTG CTCATTGCCG GGGAGTCCCG GGCGCCCGGC CCTGCCCCGA CTATTGCCGA 
AATGTGCTCA AAGGCTGCCT TGCCAACCAG GCCGACCTGG ATGCCGAGTG GAGGAACCTC 
CTGGACTCCA TGGTGCTCAT CACTGACAAG TTCTGGGGCC CGTCGGGTGC GGAGAGTGTC 
ATTGGCGGTG TGCACGTGTG GCTGGCGGAG GCCATCAACG CCCTCCAGGA CAACAAGGAC 
ACACTCACAG CTAAGGTCAT CCAGGCCTGT GGAAACCCCA AGGTCAATCC CCACGGCTCT 
GGGCCCGAGG AGAAGCGTCG CCGTGGCAAA TTGGCACTGC AGGAGAAGCC CTCCACAGGT 
ACTCTGGAAA AACTGGTCTC TGAGGCCAAG GCCCAGCTCC GAGACATTCA GGACTTCTGG 
ATCAGCCTCC CAGGGACACT GTGCAGTGAG AAGATGGCCA TGAGTCCTGC CAGTGACGAC 
CGCTGCTGGA ATGGAATTTC CAAGGGCCGG TACCTACCAG AGGTGATGGG TGACGGGCTG 
GCCAACCAGA TCAACAACCC TGAGGTGGAA GTGGACATCA CCAAGCCAGA CATGACCATC 
CGCCAGCAGA TTATGCAGCT CAAGATCATG ACCAACCGTT TACGTGGCGC CTATGGCGGC 
AACGACGTGG ACTTCCAGGA TGCTAGTGAT GACGGCAGTG GCTCCGGCAG CGGTGGCGGA 
TGCCCAGATG ACACCTGTGG CCGGAGGGTC AGCAAGAAGA GTTCCAGCTC CCGGACCCCC 
TTGACCCATG CCCTCCCCGG CCTGTCAGAA CAGGAGGGAC AGAAGACCTC AGCTGCCACC 
TGCCCAGAGC CCCACAGCTT CTTCCTGCTC TTCCTCGTCA CCTTGGTCCT TGCGGCAGCC 
AGGCCCAGGT GGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpc1 ( XM_011247925.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246227.1 Mus musculus glypican 1 (Gpc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67840
Clone ID Related Accession (Same CDS sequence)	XM_011247925.2 , XM_011247925.1
Accession Version	XM_011247925.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glypican-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGC ACCTGCGGAT CTGCCCCCAG GGCTACACTT GCTGTACTAG TGAGATGGAG 
GAGAATTTGG CCAACCACAG CCGAATGGAG CTGGAGAGCG CACTCCATGA CAGCAGCCGC 
GCCCTGCAGG CCACACTGGC CACCCAGCTG CATGGCATCG ATGACCACTT CCAGCGCCTG 
CTGAATGACT CGGAGCGCAC ACTGCAGGAG GCTTTCCCTG GGGCCTTTGG GGACCTGTAT 
ACGCAGAACA CTCGTGCCTT CCGGGACCTA TATGCTGAGC TGCGCCTCTA CTACCGTGGG 
GCCAACCTGC ACCTTGAGGA GACGCTGGCC GAGTTCTGGG CACGGCTGCT GGAGCGCCTC 
TTCAAGCAGC TGCACCCCCA GCTGCTGCCT GATGACTACC TGGACTGCCT GGGCAAGCAG 
GCGGAGGCAC TGCGGCCGTT TGGAGATGCC CCTCGAGAAC TGCGCCTGCG GGCCACCCGT 
GCCTTTGTGG CTGCACGTTC CTTTGTGCAG GGCCTGGGTG TGGCCAGTGA TGTAGTCCGG 
AAGGTGGCCC AGGTACCTCT GGCCCCAGAA TGTTCTCGGG CCATCATGAA GTTGGTCTAC 
TGTGCTCATT GCCGGGGAGT CCCGGGCGCC CGGCCCTGCC CCGACTATTG CCGAAATGTG 
CTCAAAGGCT GCCTTGCCAA CCAGGCCGAC CTGGATGCCG AGTGGAGGAA CCTCCTGGAC 
TCCATGGTGC TCATCACTGA CAAGTTCTGG GGCCCGTCGG GTGCGGAGAG TGTCATTGGC 
GGTGTGCACG TGTGGCTGGC GGAGGCCATC AACGCCCTCC AGGACAACAA GGACACACTC 
ACAGCTAAGG TCATCCAGGC CTGTGGAAAC CCCAAGGTCA ATCCCCACGG CTCTGGGCCC 
GAGGAGAAGC GTCGCCGTGG CAAATTGGCA CTGCAGGAGA AGCCCTCCAC AGGTACTCTG 
GAAAAACTGG TCTCTGAGGC CAAGGCCCAG CTCCGAGACA TTCAGGACTT CTGGATCAGC 
CTCCCAGGGA CACTGTGCAG TGAGAAGATG GCCATGAGTC CTGCCAGTGA CGACCGCTGC 
TGGAATGGAA TTTCCAAGGG CCGGTACCTA CCAGAGGTGA TGGGTGACGG GCTGGCCAAC 
CAGATCAACA ACCCTGAGGT GGAAGTGGAC ATCACCAAGC CAGACATGAC CATCCGCCAG 
CAGATTATGC AGCTCAAGAT CATGACCAAC CGTTTACGTG GCGCCTATGG CGGCAACGAC 
GTGGACTTCC AGGATGCTAG TGATGACGGC AGTGGCTCCG GCAGCGGTGG CGGATGCCCA 
GATGACACCT GTGGCCGGAG GGTCAGCAAG AAGAGTTCCA GCTCCCGGAC CCCCTTGACC 
CATGCCCTCC CCGGCCTGTC AGAACAGGAG GGACAGAAGA CCTCAGCTGC CACCTGCCCA 
GAGCCCCACA GCTTCTTCCT GCTCTTCCTC GTCACCTTGG TCCTTGCGGC AGCCAGGCCC 
AGGTGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246227.1
CDS	56..1567
Translation	MGEHLRICPQGYTCCTSEMEENLANHSRMELESALHDSSRALQATLATQLHGIDDHFQRLLNDSERTLQEAFPGAFGDLYTQNTRAFRDLYAELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLPDDYLDCLGKQAEALRPFGDAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLAPECSRAIMKLVYCAHCRGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGPSGAESVIGGVHVWLAEAINALQDNKDTLTAKVIQACGNPKVNPHGSGPEEKRRRGKLALQEKPSTGTLEKLVSEAKAQLRDIQDFWISLPGTLCSEKMAMSPASDDRCWNGISKGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRGAYGGNDVDFQDASDDGSGSGSGGGCPDDTCGRRVSKKSSSSRTPLTHALPGLSEQEGQKTSAATCPEPHSFFLLFLVTLVLAAARPRWR

Target ORF information:

RefSeq Version	XM_011247925.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glypican 1 (Gpc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247925.2

ORF Insert Sequence:

ATGGGTGAGC ACCTGCGGAT CTGCCCCCAG GGCTACACTT GCTGTACTAG TGAGATGGAG 
GAGAATTTGG CCAACCACAG CCGAATGGAG CTGGAGAGCG CACTCCATGA CAGCAGCCGC 
GCCCTGCAGG CCACACTGGC CACCCAGCTG CATGGCATCG ATGACCACTT CCAGCGCCTG 
CTGAATGACT CGGAGCGCAC ACTGCAGGAG GCTTTCCCTG GGGCCTTTGG GGACCTGTAT 
ACGCAGAACA CTCGTGCCTT CCGGGACCTA TATGCTGAGC TGCGCCTCTA CTACCGTGGG 
GCCAACCTGC ACCTTGAGGA GACGCTGGCC GAGTTCTGGG CACGGCTGCT GGAGCGCCTC 
TTCAAGCAGC TGCACCCCCA GCTGCTGCCT GATGACTACC TGGACTGCCT GGGCAAGCAG 
GCGGAGGCAC TGCGGCCGTT TGGAGATGCC CCTCGAGAAC TGCGCCTGCG GGCCACCCGT 
GCCTTTGTGG CTGCACGTTC CTTTGTGCAG GGCCTGGGTG TGGCCAGTGA TGTAGTCCGG 
AAGGTGGCCC AGGTACCTCT GGCCCCAGAA TGTTCTCGGG CCATCATGAA GTTGGTCTAC 
TGTGCTCATT GCCGGGGAGT CCCGGGCGCC CGGCCCTGCC CCGACTATTG CCGAAATGTG 
CTCAAAGGCT GCCTTGCCAA CCAGGCCGAC CTGGATGCCG AGTGGAGGAA CCTCCTGGAC 
TCCATGGTGC TCATCACTGA CAAGTTCTGG GGCCCGTCGG GTGCGGAGAG TGTCATTGGC 
GGTGTGCACG TGTGGCTGGC GGAGGCCATC AACGCCCTCC AGGACAACAA GGACACACTC 
ACAGCTAAGG TCATCCAGGC CTGTGGAAAC CCCAAGGTCA ATCCCCACGG CTCTGGGCCC 
GAGGAGAAGC GTCGCCGTGG CAAATTGGCA CTGCAGGAGA AGCCCTCCAC AGGTACTCTG 
GAAAAACTGG TCTCTGAGGC CAAGGCCCAG CTCCGAGACA TTCAGGACTT CTGGATCAGC 
CTCCCAGGGA CACTGTGCAG TGAGAAGATG GCCATGAGTC CTGCCAGTGA CGACCGCTGC 
TGGAATGGAA TTTCCAAGGG CCGGTACCTA CCAGAGGTGA TGGGTGACGG GCTGGCCAAC 
CAGATCAACA ACCCTGAGGT GGAAGTGGAC ATCACCAAGC CAGACATGAC CATCCGCCAG 
CAGATTATGC AGCTCAAGAT CATGACCAAC CGTTTACGTG GCGCCTATGG CGGCAACGAC 
GTGGACTTCC AGGATGCTAG TGATGACGGC AGTGGCTCCG GCAGCGGTGG CGGATGCCCA 
GATGACACCT GTGGCCGGAG GGTCAGCAAG AAGAGTTCCA GCTCCCGGAC CCCCTTGACC 
CATGCCCTCC CCGGCCTGTC AGAACAGGAG GGACAGAAGA CCTCAGCTGC CACCTGCCCA 
GAGCCCCACA GCTTCTTCCT GCTCTTCCTC GTCACCTTGG TCCTTGCGGC AGCCAGGCCC 
AGGTGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Alterations in mandibular morphology associated with glypican 1 and glypican 3 gene mutations.
Orthodontics & craniofacial research20(3)183-187(2017 Aug)
Mian M,Ranjitkar S,Townsend GC,Anderson PJ

Altered heparan sulfate structure in Glce(-/-) mice leads to increased Hedgehog signaling in endochondral bones.
Matrix biology : journal of the International Society for Matrix Biology4982-92(2016 Jan)
Dierker T,Bachvarova V,Krause Y,Li JP,Kjell?n L,Seidler DG,Vortkamp A

Novel insights into Notum and glypicans regulation in colorectal cancer.
Oncotarget6(38)41237-57(2015 Dec)
De Robertis M,Arigoni M,Loiacono L,Riccardo F,Calogero RA,Feodorova Y,Tashkova D,Belovejdov V,Sarafian V,Cavallo F,Signori E

Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.
Nature communications68391(2015 Sep)
Sharma P,Abbasi C,Lazic S,Teng AC,Wang D,Dubois N,Ignatchenko V,Wong V,Liu J,Araki T,Tiburcy M,Ackerley C,Zimmermann WH,Hamilton R,Sun Y,Liu PP,Keller G,Stagljar I,Scott IC,Kislinger T,Gramolini AO

PTP? functions as a presynaptic receptor for the glypican-4/LRRTM4 complex and is essential for excitatory synaptic transmission.
Proceedings of the National Academy of Sciences of the United States of America112(6)1874-9(2015 Feb)
Ko JS,Pramanik G,Um JW,Shim JS,Lee D,Kim KH,Chung GY,Condomitti G,Kim HM,Kim H,de Wit J,Park KS,Tabuchi K,Ko J

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Gpc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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