Gnai1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnai1
Entrez Gene ID	14677
Full Name	guanine nucleotide binding protein (G protein), alpha inhibiting 1
Synonyms	AU046200,Gialpha1,Gnai-1
General protein information	Preferred Names guanine nucleotide binding protein (G protein), alpha inhibiting 1 Names guanine nucleotide-binding protein G(i) subunit alpha-1 adenylate cyclase-inhibiting G alpha protein guanine nucleotide binding protein, alpha inhibiting 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 A3\|5 8.16 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010305.1	NP_034435.1	guanine nucleotide-binding protein G(i) subunit alpha-1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAI1	XP_001159548.1
Danio rerio (zebrafish)	gnai1	NP_957265.1
Rattus norvegicus (Norway rat)	Gnai1	NP_037277.1
Mus musculus (house mouse)	Gnai1	NP_034435.1
Gallus gallus (chicken)	GNAI1	NP_990734.1
Drosophila melanogaster (fruit fly)	G-ialpha65A	NP_477502.1
Xenopus tropicalis (tropical clawed frog)	gnai1	NP_001090865.1
Homo sapiens (human)	GNAI1	NP_002060.4
Bos taurus (cattle)	GNAI1	NP_776749.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005904	XP_315935.4
Caenorhabditis elegans (roundworm)	gpa-16	NP_490790.1

Gene Ontology
Bibliography

Related articles in PubMed

Proinflammatory switch from G?s to G?i signaling by Glucagon-like peptide-1 receptor in murine splenic monocyte following burn injury.
Zhang QH, Hao JW, Li GL, Ji XJ, Yao XD, Dong N, Yao YM
Inflammation research : official journal of the European Histamine Research Society ... [et al.]67(2)157-168(2018 Feb)

Differential effects of inhibitory G-protein isoforms on G-protein gated inwardly rectifying K
Nobles M, Montaigne D, Sebastian S, Birnbaumer L, Tinker A
American journal of physiology. Cell physiology(2018 Jan)

A link between planar polarity and staircase-like bundle architecture in hair cells.
Tarchini B, Tadenev AL, Devanney N, Cayouette M
Development (Cambridge, England)143(21)3926-3932(2016 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LGN and Galphai participate in a long-inferred signal that originates outside the bundle to model its staircase-like architecture, a property that is essential for direction sensitivity to mechanical deflection and hearing.
Title: A link between planar polarity and staircase-like bundle architecture in hair cells.

Gnai1 function is impaired in the spinal cord of Ews/Ewsr1 KO mice
Title: Integrated analysis of omics data using microRNA-target mRNA network and PPI network reveals regulation of Gnai1 function in the spinal cord of Ews/Ewsr1 KO mice.

Gnai1 missense mutation is responsible of hyperpigmentation in mouse model.
Title: Gnaq(M1J): An ENU-Induced Mutant Allele Affecting Pigmentation in the Mouse.

stimulation of GPR17 by the small molecule agonist MDL29,951 (2-carboxy-4,6-dichloro-1H-indole-3-propionic acid) decreases myelin basic protein expression levels mainly by triggering the Galphai/o signaling pathway.
Title: The Orphan G Protein-coupled Receptor GPR17 Negatively Regulates Oligodendrocyte Differentiation via G?i/o and Its Downstream Effector Molecules.

acidosis in inflamed tissues may be a decisive factor to regulate switching of PKA and PKCepsilon dependence via proton-sensing G-protein-coupled receptors.
Title: Acidosis Mediates the Switching of Gs-PKA and Gi-PKC? Dependence in Prolonged Hyperalgesia Induced by Inflammation.

The following Gnai1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnai1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17842	NM_010305.1 Latest version!	Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 1 (Gnai1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnai1 ( NM_010305.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034435.1 Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 1 (Gnai1), mRNA.

Price & Availability↑

CloneID	OMu17842
Clone ID Related Accession (Same CDS sequence)	NM_010305.1
Accession Version	NM_010305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	guanine nucleotide-binding protein G(i) subunit alpha-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY740128.1, BC032995.1 and AC116505.13. On Sep 9, 2005 this sequence version replaced XM_355574.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.155418.1, SRR1660815.81276.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTGCA CATTGAGCGC TGAGGACAAG GCGGCCGTGG AGCGCAGCAA GATGATCGAC 
CGCAACCTCC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TCAAGCTGCT GCTGCTGGGT 
GCTGGGGAAT CTGGAAAGAG TACCATTGTG AAGCAGATGA AGATTATCCA CGAAGCCGGC 
TACTCGGAAG AGGAGTGTAA GCAGTACAAG GCAGTGGTCT ACAGCAACAC TATCCAGTCC 
ATCATTGCCA TCATTAGAGC CATGGGGAGG TTGAAAATCG ACTTCGGAGA CTCTGCTCGG 
GCGGATGATG CTCGCCAACT TTTCGTGCTT GCTGGGGCTG CAGAAGAAGG CTTTATGACT 
GCAGAGCTCG CCGGTGTCAT AAAGAGACTG TGGAAAGACA GTGGTGTGCA AGCCTGCTTC 
AACAGATCCC GGGAGTACCA GCTGAACGAT TCGGCAGCGT ACTATCTGAA TGACTTGGAC 
AGAATAGCAC AGCCAAATTA CATCCCAACT CAGCAGGATG TCCTCAGAAC CAGAGTGAAG 
ACCACAGGGA TTGTGGAAAC CCACTTTACC TTCAAAGATC TTCATTTTAA AATGTTTGAC 
GTGGGAGGTC AGAGGTCAGA GCGGAAGAAG TGGATCCACT GCTTTGAAGG GGTGACCGCC 
ATCATCTTCT GTGTGGCCCT GAGTGACTAT GACCTGGTTC TTGCTGAAGA TGAAGAAATG 
AACCGTATGC ACGAGAGCAT GAAGCTGTTC GATAGCATCT GTAACAACAA GTGGTTTACA 
GACACGTCCA TCATCCTTTT CCTCAACAAG AAGGACCTCT TCGAAGAAAA AATAAAAAAG 
AGCCCCCTCA CGATATGCTA CCCAGAATAT GCAGGCTCAA ACACATATGA AGAAGCGGCC 
GCGTATATTC AGTGTCAGTT TGAAGACCTC AATAAAAGGA AGGACACAAA GGAAATTTAC 
ACCCACTTCA CGTGCGCCAC AGATACGAAG AACGTGCAGT TCGTGTTCGA TGCTGTAACA 
GACGTCATCA TAAAGAATAA CCTAAAAGAC TGTGGTCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034435.1
CDS	245..1309
Translation	MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	NM_010305.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanine nucleotide binding protein (G protein), alpha inhibiting 1 (Gnai1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010305.1

ORF Insert Sequence:

ATGGGCTGCA CATTGAGCGC TGAGGACAAG GCGGCCGTGG AGCGCAGCAA GATGATCGAC 
CGCAACCTCC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TCAAGCTGCT GCTGCTGGGT 
GCTGGGGAAT CTGGAAAGAG TACCATTGTG AAGCAGATGA AGATTATCCA CGAAGCCGGC 
TACTCGGAAG AGGAGTGTAA GCAGTACAAG GCAGTGGTCT ACAGCAACAC TATCCAGTCC 
ATCATTGCCA TCATTAGAGC CATGGGGAGG TTGAAAATCG ACTTCGGAGA CTCTGCTCGG 
GCGGATGATG CTCGCCAACT TTTCGTGCTT GCTGGGGCTG CAGAAGAAGG CTTTATGACT 
GCAGAGCTCG CCGGTGTCAT AAAGAGACTG TGGAAAGACA GTGGTGTGCA AGCCTGCTTC 
AACAGATCCC GGGAGTACCA GCTGAACGAT TCGGCAGCGT ACTATCTGAA TGACTTGGAC 
AGAATAGCAC AGCCAAATTA CATCCCAACT CAGCAGGATG TCCTCAGAAC CAGAGTGAAG 
ACCACAGGGA TTGTGGAAAC CCACTTTACC TTCAAAGATC TTCATTTTAA AATGTTTGAC 
GTGGGAGGTC AGAGGTCAGA GCGGAAGAAG TGGATCCACT GCTTTGAAGG GGTGACCGCC 
ATCATCTTCT GTGTGGCCCT GAGTGACTAT GACCTGGTTC TTGCTGAAGA TGAAGAAATG 
AACCGTATGC ACGAGAGCAT GAAGCTGTTC GATAGCATCT GTAACAACAA GTGGTTTACA 
GACACGTCCA TCATCCTTTT CCTCAACAAG AAGGACCTCT TCGAAGAAAA AATAAAAAAG 
AGCCCCCTCA CGATATGCTA CCCAGAATAT GCAGGCTCAA ACACATATGA AGAAGCGGCC 
GCGTATATTC AGTGTCAGTT TGAAGACCTC AATAAAAGGA AGGACACAAA GGAAATTTAC 
ACCCACTTCA CGTGCGCCAC AGATACGAAG AACGTGCAGT TCGTGTTCGA TGCTGTAACA 
GACGTCATCA TAAAGAATAA CCTAAAAGAC TGTGGTCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proinflammatory switch from G?s to G?i signaling by Glucagon-like peptide-1 receptor in murine splenic monocyte following burn injury.
Inflammation research : official journal of the European Histamine Research Society ... [et al.]67(2)157-168(2018 Feb)
Zhang QH,Hao JW,Li GL,Ji XJ,Yao XD,Dong N,Yao YM

Differential effects of inhibitory G-protein isoforms on G-protein gated inwardly rectifying K
American journal of physiology. Cell physiology(2018 Jan)
Nobles M,Montaigne D,Sebastian S,Birnbaumer L,Tinker A

A link between planar polarity and staircase-like bundle architecture in hair cells.
Development (Cambridge, England)143(21)3926-3932(2016 11)
Tarchini B,Tadenev AL,Devanney N,Cayouette M

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Gnai1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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