Glud1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Glud1
Entrez Gene ID	14661
Full Name	glutamate dehydrogenase 1
Synonyms	AI118167,Gdh-X,Glud,Gludl
General protein information	Preferred Names glutamate dehydrogenase 1 Names glutamate dehydrogenase 1, mitochondrial GDH 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 B\|14 20.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008133.4	NP_032159.1	glutamate dehydrogenase 1, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GLUD1	XP_001137904.1
Bos taurus (cattle)	GLUD1	XP_005226606.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004362	XP_003436795.1
Arabidopsis thaliana (thale cress)	GDH2	NP_001119183.1
Oryza sativa (rice)	Os02g0650900	NP_001047589.1
Xenopus tropicalis (tropical clawed frog)	LOC100496343	XP_002941808.2
Canis lupus familiaris (dog)	GLUD1	XP_005619499.1
Danio rerio (zebrafish)	glud1b	NP_955839.2
Homo sapiens (human)	GLUD1	NP_005262.1
Mus musculus (house mouse)	Glud1	NP_032159.1
Rattus norvegicus (Norway rat)	Glud1	NP_036702.1
Danio rerio (zebrafish)	glud1a	NP_997741.1
Drosophila melanogaster (fruit fly)	Gdh	NP_524470.4
Xenopus tropicalis (tropical clawed frog)	glud1	NP_001011138.1
	GLUD1	XP_002805686.1
Gallus gallus (chicken)	GLUD1	XP_421497.3
Caenorhabditis elegans (roundworm)	gdh-1	NP_502267.1

Gene Ontology
Bibliography

Related articles in PubMed

Glutamine-Elicited Secretion of Glucagon-Like Peptide 1 (GLP-1) is Governed by an Activated Glutamate Dehydrogenase.
Andersson LE, Shcherbina L, Al-Majdoub M, Vishnu N, Arroyo CB, Carrara JA, Wollheim CB, Fex M, Mulder H, Wierup N, Sp?gel P
Diabetes(2017 Dec)

Identification of the molecular dysfunction caused by glutamate dehydrogenase S445L mutation responsible for hyperinsulinism/hyperammonemia.
Grimaldi M, Karaca M, Latini L, Brioudes E, Schalch T, Maechler P
Human molecular genetics26(18)3453-3465(2017 Sep)

Glutamate dehydrogenase is essential to sustain neuronal oxidative energy metabolism during stimulation.
Hohnholt MC, Andersen VH, Andersen JV, Christensen SK, Karaca M, Maechler P, Waagepetersen HS
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism271678X17714680(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mRNA and protein levels of GluD1 were increased in fetal brain of foxg1(+/-) mice. mRNA and protein levels of GluD1 were decreased in adult brain of foxg1(+/-) mice.
Title: Imbalance of excitatory/inhibitory synaptic protein expression in iPSC-derived neurons from FOXG1(+/-) patients and in foxg1(+/-) mice.

lack of glutamate oxidation in brain-specific GDH null CnsGlud1-/- mice resulted in a central energy-deprivation state with increased ADP/ATP ratios and phospho-AMPK in the hypothalamus.
Title: GDH-Dependent Glutamate Oxidation in the Brain Dictates Peripheral Energy Substrate Distribution.

This study demonstrated a critical requirement for GluD1 in normal spine development in the cortex and hippocampus.
Title: Essential role of GluD1 in dendritic spine development and GluN2B to GluN2A NMDAR subunit switch in the cortex and hippocampus reveals ability of GluN2B inhibition in correcting hyperconnectivity.

The GDH activity in mice is highest in the liver with NAD(+) as a coenzyme and highest GDH activity was determined at a glutamate concentration of 10 mM.
Title: Determination of glutamate dehydrogenase activity and its kinetics in mouse tissues using metabolic mapping (quantitative enzyme histochemistry).

GluD1 regulates the connectivity of parallel fiber-interneuron synapses and promotes differentiation of interneurons.
Title: Enriched expression of GluD1 in higher brain regions and its involvement in parallel fiber-interneuron synapse formation in the cerebellum.

The following Glud1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Glud1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22775	NM_008133.4 Latest version!	Mus musculus glutamate dehydrogenase 1 (Glud1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$328.30 $469.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Glud1 ( NM_008133.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032159.1 Mus musculus glutamate dehydrogenase 1 (Glud1), mRNA.

Price & Availability↑

CloneID	OMu22775
Clone ID Related Accession (Same CDS sequence)	NM_008133.4
Accession Version	NM_008133.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	glutamate dehydrogenase 1, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK150637.1, AK089152.1 and AK161876.1. On Mar 21, 2009 this sequence version replaced NM_008133.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK150637.1, BC052724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTACCGCC GTCTGGGCGA AGCGCTGCTG CTGTCCCGCG CCGGGCCCGC TGCCCTGGGC 
TCTGCGGCTG CAGACTCAGC CGCGCTGCTG GGCTGGGCCC GCGGACAGCC CTCCGCCGCC 
CCGCAACCCG GGCTCACGCC GGTCGCCCGG CGCCACTACA GCGAAGCGGC CGCCGACCGC 
GAAGACGACC CCAACTTCTT CAAGATGGTG GAGGGCTTCT TCGACCGCGG CGCCAGCATC 
GTAGAGGACA AGCTGGTGGA AGACCTGAAG ACCCGGGAGA GCGAGGAGCA GAAGCGGAAC 
CGGGTGCGCG GCATCCTGAG GATCATCAAG CCCTGCAACC ATGTGTTGAG CCTCTCCTTC 
CCCATCCGGC GCGACGACGG CTCCTGGGAG GTCATCGAAG GCTACCGGGC CCAGCACAGC 
CAGCACCGCA CGCCCTGCAA GGGAGGTATC CGTTACAGCA CTGACGTGAG TGTGGATGAA 
GTAAAAGCAC TGGCTTCCTT AATGACATAC AAGTGCGCTG TGGTCGATGT ACCGTTTGGA 
GGTGCTAAAG CAGGCGTTAA GATCAACCCC AAGAACTATA CAGATAATGA ATTAGAAAAA 
ATTACACGGA GGTTCACTAT GGAGCTGGCC AAGAAGGGTT TTATTGGTCC TGGCATTGAT 
GTGCCTGCCC CAGACATGAG CACGGGTGAG CGGGAGATGT CCTGGATCGC TGACACCTAT 
GCCAGCACCA TAGGGCACTA TGATATCAAT GCGCATGCCT GTGTTACTGG GAAACCCATC 
AGTCAAGGAG GCATCCACGG GCGCATCTCC GCTACTGGCC GGGGTGTCTT CCATGGAATT 
GAAAACTTCA TCAATGAGGC TTCTTACATG AGCATTTTAG GAATGACACC AGGCTTTGGC 
GATAAGACAT TTGTTGTTCA GGGATTTGGT AATGTGGGCC TGCACTCTAT GAGATATTTA 
CATCGTTTTG GTGCTAAATG TGTTGGTGTT GGAGAGTCTG ATGGGAGTAT ATGGAATCCG 
GATGGGATTG ACCCAAAAGA ACTGGAAGAC TTCAAGTTGC AACATGGATC AATTCTGGGC 
TTCCCCAAAG CCAAGGTCTA TGAAGGAAGC ATCTTGGAGG CTGACTGTGA CATTCTGATT 
CCTGCCGCCA GCGAGAAGCA GTTGACCAAA TCCAATGCAC CCAGAGTCAA AGCCAAGATC 
ATTGCTGAAG GTGCCAATGG GCCAACCACT CCAGAGGCTG ATAAGATTTT CCTGGAAAGA 
AACATCATGG TTATTCCCGA TCTCTACTTA AATGCTGGAG GAGTAACAGT GTCTTACTTT 
GAGTGGCTAA AGAATCTAAA TCATGTCAGC TACGGCCGAT TGACCTTCAA ATATGAAAGG 
GACTCTAACT ACCACTTGCT CATGTCTGTT CAAGAGAGTT TAGAGAGAAA GTTTGGAAAG 
CATGGTGGAA CTATTCCTGT GGTCCCCACA GCAGAGTTCC AGGACAGGAT ATCGGGTGCA 
TCTGAGAAAG ACATTGTGCA CTCTGGCTTG GCCTACACAA TGGAGAGATC TGCCAGGCAA 
ATTATGCGCA CAGCCATGAA GTATAACCTG GGATTGGACC TGAGAACAGC TGCCTATGTC 
AATGCTATCG AGAAAGTCTT CAAGGTGTAC AATGAAGCTG GTGTGACCTT CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032159.1
CDS	172..1848
Translation	MYRRLGEALLLSRAGPAALGSAAADSAALLGWARGQPSAAPQPGLTPVARRHYSEAAADREDDPNFFKMVEGFFDRGASIVEDKLVEDLKTRESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTMELAKKGFIGPGIDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFGDKTFVVQGFGNVGLHSMRYLHRFGAKCVGVGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKAKVYEGSILEADCDILIPAASEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLERNIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLMSVQESLERKFGKHGGTIPVVPTAEFQDRISGASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTFT

Target ORF information:

RefSeq Version	NM_008133.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate dehydrogenase 1 (Glud1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008133.4

ORF Insert Sequence:

ATGTACCGCC GTCTGGGCGA AGCGCTGCTG CTGTCCCGCG CCGGGCCCGC TGCCCTGGGC 
TCTGCGGCTG CAGACTCAGC CGCGCTGCTG GGCTGGGCCC GCGGACAGCC CTCCGCCGCC 
CCGCAACCCG GGCTCACGCC GGTCGCCCGG CGCCACTACA GCGAAGCGGC CGCCGACCGC 
GAAGACGACC CCAACTTCTT CAAGATGGTG GAGGGCTTCT TCGACCGCGG CGCCAGCATC 
GTAGAGGACA AGCTGGTGGA AGACCTGAAG ACCCGGGAGA GCGAGGAGCA GAAGCGGAAC 
CGGGTGCGCG GCATCCTGAG GATCATCAAG CCCTGCAACC ATGTGTTGAG CCTCTCCTTC 
CCCATCCGGC GCGACGACGG CTCCTGGGAG GTCATCGAAG GCTACCGGGC CCAGCACAGC 
CAGCACCGCA CGCCCTGCAA GGGAGGTATC CGTTACAGCA CTGACGTGAG TGTGGATGAA 
GTAAAAGCAC TGGCTTCCTT AATGACATAC AAGTGCGCTG TGGTCGATGT ACCGTTTGGA 
GGTGCTAAAG CAGGCGTTAA GATCAACCCC AAGAACTATA CAGATAATGA ATTAGAAAAA 
ATTACACGGA GGTTCACTAT GGAGCTGGCC AAGAAGGGTT TTATTGGTCC TGGCATTGAT 
GTGCCTGCCC CAGACATGAG CACGGGTGAG CGGGAGATGT CCTGGATCGC TGACACCTAT 
GCCAGCACCA TAGGGCACTA TGATATCAAT GCGCATGCCT GTGTTACTGG GAAACCCATC 
AGTCAAGGAG GCATCCACGG GCGCATCTCC GCTACTGGCC GGGGTGTCTT CCATGGAATT 
GAAAACTTCA TCAATGAGGC TTCTTACATG AGCATTTTAG GAATGACACC AGGCTTTGGC 
GATAAGACAT TTGTTGTTCA GGGATTTGGT AATGTGGGCC TGCACTCTAT GAGATATTTA 
CATCGTTTTG GTGCTAAATG TGTTGGTGTT GGAGAGTCTG ATGGGAGTAT ATGGAATCCG 
GATGGGATTG ACCCAAAAGA ACTGGAAGAC TTCAAGTTGC AACATGGATC AATTCTGGGC 
TTCCCCAAAG CCAAGGTCTA TGAAGGAAGC ATCTTGGAGG CTGACTGTGA CATTCTGATT 
CCTGCCGCCA GCGAGAAGCA GTTGACCAAA TCCAATGCAC CCAGAGTCAA AGCCAAGATC 
ATTGCTGAAG GTGCCAATGG GCCAACCACT CCAGAGGCTG ATAAGATTTT CCTGGAAAGA 
AACATCATGG TTATTCCCGA TCTCTACTTA AATGCTGGAG GAGTAACAGT GTCTTACTTT 
GAGTGGCTAA AGAATCTAAA TCATGTCAGC TACGGCCGAT TGACCTTCAA ATATGAAAGG 
GACTCTAACT ACCACTTGCT CATGTCTGTT CAAGAGAGTT TAGAGAGAAA GTTTGGAAAG 
CATGGTGGAA CTATTCCTGT GGTCCCCACA GCAGAGTTCC AGGACAGGAT ATCGGGTGCA 
TCTGAGAAAG ACATTGTGCA CTCTGGCTTG GCCTACACAA TGGAGAGATC TGCCAGGCAA 
ATTATGCGCA CAGCCATGAA GTATAACCTG GGATTGGACC TGAGAACAGC TGCCTATGTC 
AATGCTATCG AGAAAGTCTT CAAGGTGTAC AATGAAGCTG GTGTGACCTT CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glutamine-Elicited Secretion of Glucagon-Like Peptide 1 (GLP-1) is Governed by an Activated Glutamate Dehydrogenase.
Diabetes(2017 Dec)
Andersson LE,Shcherbina L,Al-Majdoub M,Vishnu N,Arroyo CB,Carrara JA,Wollheim CB,Fex M,Mulder H,Wierup N,Sp?gel P

Identification of the molecular dysfunction caused by glutamate dehydrogenase S445L mutation responsible for hyperinsulinism/hyperammonemia.
Human molecular genetics26(18)3453-3465(2017 Sep)
Grimaldi M,Karaca M,Latini L,Brioudes E,Schalch T,Maechler P

Glutamate dehydrogenase is essential to sustain neuronal oxidative energy metabolism during stimulation.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism271678X17714680(2017 Jan)
Hohnholt MC,Andersen VH,Andersen JV,Christensen SK,Karaca M,Maechler P,Waagepetersen HS

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Glud1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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