Gjb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gjb1
Entrez Gene ID	14618
Full Name	gap junction protein, beta 1
Synonyms	Cnx32,Cx32,connexin-32
General protein information	Preferred Names gap junction protein, beta 1 Names gap junction beta-1 protein connexin 32 connexin-43 gap junction membrane channel protein beta 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X D\|X 44.06 cM
Summary	This gene is a member of the gap junction protein (connexin) family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of ions and small molecules between cells. Mutations in a similar gene in human cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Oct 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001302498.1	NP_001289427.1	gap junction beta-1 protein
XM_030251227.1	XP_030107087.1	gap junction beta-1 protein isoform X1
NM_001302496.1	NP_001289425.1	gap junction beta-1 protein
NM_008124.3	NP_032150.2	gap junction beta-1 protein
NM_001302497.1	NP_001289426.1	gap junction beta-1 protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GJB1	XP_538073.1
Xenopus tropicalis (tropical clawed frog)	gjb1	NP_001001240.1
Homo sapiens (human)	GJB1	NP_000157.1
Rattus norvegicus (Norway rat)	Gjb1	NP_058947.1
Pan troglodytes (chimpanzee)	GJB1	XP_521117.1
Bos taurus (cattle)	GJB1	NP_776494.1
Gallus gallus (chicken)	GJB1	NP_989702.1
	GJB1	XP_001101168.1
Mus musculus (house mouse)	Gjb1	NP_032150.2
Danio rerio (zebrafish)	cx31.7	XP_001921623.4

Gene Ontology
Bibliography

Related articles in PubMed

Connexin43, but not connexin30, contributes to adult neurogenesis in the dentate gyrus.
Zhang J, Griemsmann S, Wu Z, Dobrowolski R, Willecke K, Theis M, Steinh?user C, Bedner P
Brain research bulletin13691-100(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Blocking of connexin32 or connexin43 hemichannels decreased serum levels of pro-inflammatory cytokines, and reduced acetaminophen-induced liver injury.
Title: Connexin hemichannel inhibition reduces acetaminophen-induced liver injury in mice.

The results of this study indicated that the Leu89Pro substitution in the second transmembrane domain of CX32 disrupts the trafficking of the protein, inhibiting the assembly of CX32 gap junctions, which in turn may result in peripheral neuropathy.
Title: Aberrant trafficking of a Leu89Pro connexin32 mutant associated with X-linked dominant Charcot-Marie-Tooth disease.

We show that the cell-surface and secreted isoforms of CSF-1 have opposing effects on macrophage activation and disease progression in a mouse model of connexin32-deficientconnexin32-deficient mice
Title: Cell-Surface and Secreted Isoforms of CSF-1 Exert Opposing Roles in Macrophage-Mediated Neural Damage in Cx32-Deficient Mice.

Results identify CSF-1-activated macrophages as crucial mediators of detrimental Schwann cell dedifferentiation in Cx32-deficient mice
Title: CSF-1-activated macrophages are target-directed and essential mediators of Schwann cell dedifferentiation and dysfunction in Cx32-deficient mice.

These findings support a role for Cx32 in non-myelinating and regenerating populations of Schwann cells in normal axonal maintenance in re-myelination, and regeneration of peripheral nerve following injury
Title: Gene expression profiling studies in regenerating nerves in a mouse model for CMT1X: uninjured Cx32-knockout peripheral nerves display expression profile of injured wild type nerves.

The following Gjb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gjb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01388	NM_001302497.1 Latest version!	Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu01388	NM_008124.3 Latest version!	Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu01388	NM_001302498.1 Latest version!	Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu01388	NM_001302496.1 Latest version!	Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu01388	XM_030251227.1 Latest version!	Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gjb1 ( NM_001302498.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289427.1 Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu01388
Clone ID Related Accession (Same CDS sequence)	NM_001302498.1 , XM_030251227.1 , NM_001302496.1 , NM_008124.3 , NM_001302497.1
Accession Version	NM_001302498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	gap junction beta-1 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X84215.1, BY087537.1, AK134742.1, AA423068.1, AL683892.12 and AI849881.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. All of the variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X84215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGGA CAGGTCTATA CACCTTGCTC AGTGGCGTGA ATCGGCACTC TACAGCCATT 
GGCCGAGTAT GGCTGTCTGT CATCTTCATC TTCAGAATCA TGGTGCTGGT GGTGGCTGCT 
GAGAGCGTGT GGGGGGATGA GAAGTCCTCT TTCATCTGTA ACACCCTCCA GCCGGGCTGC 
AACAGCGTCT GCTATGACCA TTTTTTCCCC ATCTCCCACG TGCGCCTATG GTCCCTGCAG 
CTTATCTTGG TTTCCACCCC AGCTCTCCTC GTGGCAATGC ACGTAGCTCA CCAACAGCAC 
ATAGAAAAGA AAATGCTACG GCTTGAGGGG CATGGGGACC CCCTTCACCT GGAAGAGGTA 
AAGAGACACA AGGTGCACAT CTCAGGGACA CTGTGGTGGA CCTATGTCAT CAGTGTGGTG 
TTCCGGCTGC TGTTCGAGGC TGTCTTCATG TATGTCTTCT ATCTGCTCTA CCCCGGCTAT 
GCCATGGTGC GGCTGGTCAA GTGTGAAGCC TTCCCCTGCC CCAACACAGT GGACTGCTTC 
GTGTCCCGCC CCACCGAGAA AACCGTCTTC ACTGTCTTTA TGCTCGCAGC CTCCGGCATC 
TGCATTATCC TCAACGTGGC GGAGGTGGTG TACCTCATCA TCCGGGCCTG TGCCCGCCGT 
GCTCAGCGCC GCTCCAATCC GCCCTCCCGC AAGGGCTCGG GCTTCGGCCA CCGCCTCTCA 
CCTGAATACA AGCAGAATGA GATCAACAAG CTGCTGAGCG AGCAGGATGG CTCTCTGAAA 
GACATACTGC GCCGCAGCCC TGGCACAGGG GCCGGGCTCG CTGAAAAGAG CGACCGATGC 
TCAGCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289427.1
CDS	75..926
Translation	MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDHFFPISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCEAFPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC

Target ORF information:

RefSeq Version	NM_001302498.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302498.1

ORF Insert Sequence:

ATGAACTGGA CAGGTCTATA CACCTTGCTC AGTGGCGTGA ATCGGCACTC TACAGCCATT 
GGCCGAGTAT GGCTGTCTGT CATCTTCATC TTCAGAATCA TGGTGCTGGT GGTGGCTGCT 
GAGAGCGTGT GGGGGGATGA GAAGTCCTCT TTCATCTGTA ACACCCTCCA GCCGGGCTGC 
AACAGCGTCT GCTATGACCA TTTTTTCCCC ATCTCCCACG TGCGCCTATG GTCCCTGCAG 
CTTATCTTGG TTTCCACCCC AGCTCTCCTC GTGGCAATGC ACGTAGCTCA CCAACAGCAC 
ATAGAAAAGA AAATGCTACG GCTTGAGGGG CATGGGGACC CCCTTCACCT GGAAGAGGTA 
AAGAGACACA AGGTGCACAT CTCAGGGACA CTGTGGTGGA CCTATGTCAT CAGTGTGGTG 
TTCCGGCTGC TGTTCGAGGC TGTCTTCATG TATGTCTTCT ATCTGCTCTA CCCCGGCTAT 
GCCATGGTGC GGCTGGTCAA GTGTGAAGCC TTCCCCTGCC CCAACACAGT GGACTGCTTC 
GTGTCCCGCC CCACCGAGAA AACCGTCTTC ACTGTCTTTA TGCTCGCAGC CTCCGGCATC 
TGCATTATCC TCAACGTGGC GGAGGTGGTG TACCTCATCA TCCGGGCCTG TGCCCGCCGT 
GCTCAGCGCC GCTCCAATCC GCCCTCCCGC AAGGGCTCGG GCTTCGGCCA CCGCCTCTCA 
CCTGAATACA AGCAGAATGA GATCAACAAG CTGCTGAGCG AGCAGGATGG CTCTCTGAAA 
GACATACTGC GCCGCAGCCC TGGCACAGGG GCCGGGCTCG CTGAAAAGAG CGACCGATGC 
TCAGCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gjb1 ( NM_001302496.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289425.1 Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01388
Clone ID Related Accession (Same CDS sequence)	NM_001302498.1 , XM_030251227.1 , NM_001302496.1 , NM_008124.3 , NM_001302497.1
Accession Version	NM_001302496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	gap junction beta-1 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL683892.12, BY087537.1, AK134742.1, AA423068.1 and AI849881.1. On Oct 24, 2014 this sequence version replaced XM_006527826.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All of the variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF551619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGGA CAGGTCTATA CACCTTGCTC AGTGGCGTGA ATCGGCACTC TACAGCCATT 
GGCCGAGTAT GGCTGTCTGT CATCTTCATC TTCAGAATCA TGGTGCTGGT GGTGGCTGCT 
GAGAGCGTGT GGGGGGATGA GAAGTCCTCT TTCATCTGTA ACACCCTCCA GCCGGGCTGC 
AACAGCGTCT GCTATGACCA TTTTTTCCCC ATCTCCCACG TGCGCCTATG GTCCCTGCAG 
CTTATCTTGG TTTCCACCCC AGCTCTCCTC GTGGCAATGC ACGTAGCTCA CCAACAGCAC 
ATAGAAAAGA AAATGCTACG GCTTGAGGGG CATGGGGACC CCCTTCACCT GGAAGAGGTA 
AAGAGACACA AGGTGCACAT CTCAGGGACA CTGTGGTGGA CCTATGTCAT CAGTGTGGTG 
TTCCGGCTGC TGTTCGAGGC TGTCTTCATG TATGTCTTCT ATCTGCTCTA CCCCGGCTAT 
GCCATGGTGC GGCTGGTCAA GTGTGAAGCC TTCCCCTGCC CCAACACAGT GGACTGCTTC 
GTGTCCCGCC CCACCGAGAA AACCGTCTTC ACTGTCTTTA TGCTCGCAGC CTCCGGCATC 
TGCATTATCC TCAACGTGGC GGAGGTGGTG TACCTCATCA TCCGGGCCTG TGCCCGCCGT 
GCTCAGCGCC GCTCCAATCC GCCCTCCCGC AAGGGCTCGG GCTTCGGCCA CCGCCTCTCA 
CCTGAATACA AGCAGAATGA GATCAACAAG CTGCTGAGCG AGCAGGATGG CTCTCTGAAA 
GACATACTGC GCCGCAGCCC TGGCACAGGG GCCGGGCTCG CTGAAAAGAG CGACCGATGC 
TCAGCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289425.1
CDS	54..905
Translation	MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDHFFPISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCEAFPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC

Target ORF information:

RefSeq Version	NM_001302496.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302496.1

ORF Insert Sequence:

ATGAACTGGA CAGGTCTATA CACCTTGCTC AGTGGCGTGA ATCGGCACTC TACAGCCATT 
GGCCGAGTAT GGCTGTCTGT CATCTTCATC TTCAGAATCA TGGTGCTGGT GGTGGCTGCT 
GAGAGCGTGT GGGGGGATGA GAAGTCCTCT TTCATCTGTA ACACCCTCCA GCCGGGCTGC 
AACAGCGTCT GCTATGACCA TTTTTTCCCC ATCTCCCACG TGCGCCTATG GTCCCTGCAG 
CTTATCTTGG TTTCCACCCC AGCTCTCCTC GTGGCAATGC ACGTAGCTCA CCAACAGCAC 
ATAGAAAAGA AAATGCTACG GCTTGAGGGG CATGGGGACC CCCTTCACCT GGAAGAGGTA 
AAGAGACACA AGGTGCACAT CTCAGGGACA CTGTGGTGGA CCTATGTCAT CAGTGTGGTG 
TTCCGGCTGC TGTTCGAGGC TGTCTTCATG TATGTCTTCT ATCTGCTCTA CCCCGGCTAT 
GCCATGGTGC GGCTGGTCAA GTGTGAAGCC TTCCCCTGCC CCAACACAGT GGACTGCTTC 
GTGTCCCGCC CCACCGAGAA AACCGTCTTC ACTGTCTTTA TGCTCGCAGC CTCCGGCATC 
TGCATTATCC TCAACGTGGC GGAGGTGGTG TACCTCATCA TCCGGGCCTG TGCCCGCCGT 
GCTCAGCGCC GCTCCAATCC GCCCTCCCGC AAGGGCTCGG GCTTCGGCCA CCGCCTCTCA 
CCTGAATACA AGCAGAATGA GATCAACAAG CTGCTGAGCG AGCAGGATGG CTCTCTGAAA 
GACATACTGC GCCGCAGCCC TGGCACAGGG GCCGGGCTCG CTGAAAAGAG CGACCGATGC 
TCAGCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gjb1 ( NM_008124.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032150.2 Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01388
Clone ID Related Accession (Same CDS sequence)	NM_001302498.1 , XM_030251227.1 , NM_001302496.1 , NM_008124.3 , NM_001302497.1
Accession Version	NM_008124.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	gap junction beta-1 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL683892.12, BY105229.1, BY087537.1, AK134742.1, AA423068.1 and AI849881.1. On Oct 24, 2014 this sequence version replaced NM_008124.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026833.1, BI218075.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGGA CAGGTCTATA CACCTTGCTC AGTGGCGTGA ATCGGCACTC TACAGCCATT 
GGCCGAGTAT GGCTGTCTGT CATCTTCATC TTCAGAATCA TGGTGCTGGT GGTGGCTGCT 
GAGAGCGTGT GGGGGGATGA GAAGTCCTCT TTCATCTGTA ACACCCTCCA GCCGGGCTGC 
AACAGCGTCT GCTATGACCA TTTTTTCCCC ATCTCCCACG TGCGCCTATG GTCCCTGCAG 
CTTATCTTGG TTTCCACCCC AGCTCTCCTC GTGGCAATGC ACGTAGCTCA CCAACAGCAC 
ATAGAAAAGA AAATGCTACG GCTTGAGGGG CATGGGGACC CCCTTCACCT GGAAGAGGTA 
AAGAGACACA AGGTGCACAT CTCAGGGACA CTGTGGTGGA CCTATGTCAT CAGTGTGGTG 
TTCCGGCTGC TGTTCGAGGC TGTCTTCATG TATGTCTTCT ATCTGCTCTA CCCCGGCTAT 
GCCATGGTGC GGCTGGTCAA GTGTGAAGCC TTCCCCTGCC CCAACACAGT GGACTGCTTC 
GTGTCCCGCC CCACCGAGAA AACCGTCTTC ACTGTCTTTA TGCTCGCAGC CTCCGGCATC 
TGCATTATCC TCAACGTGGC GGAGGTGGTG TACCTCATCA TCCGGGCCTG TGCCCGCCGT 
GCTCAGCGCC GCTCCAATCC GCCCTCCCGC AAGGGCTCGG GCTTCGGCCA CCGCCTCTCA 
CCTGAATACA AGCAGAATGA GATCAACAAG CTGCTGAGCG AGCAGGATGG CTCTCTGAAA 
GACATACTGC GCCGCAGCCC TGGCACAGGG GCCGGGCTCG CTGAAAAGAG CGACCGATGC 
TCAGCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032150.2
CDS	142..993
Translation	MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDHFFPISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCEAFPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC

Target ORF information:

RefSeq Version	NM_008124.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008124.3

ORF Insert Sequence:

ATGAACTGGA CAGGTCTATA CACCTTGCTC AGTGGCGTGA ATCGGCACTC TACAGCCATT 
GGCCGAGTAT GGCTGTCTGT CATCTTCATC TTCAGAATCA TGGTGCTGGT GGTGGCTGCT 
GAGAGCGTGT GGGGGGATGA GAAGTCCTCT TTCATCTGTA ACACCCTCCA GCCGGGCTGC 
AACAGCGTCT GCTATGACCA TTTTTTCCCC ATCTCCCACG TGCGCCTATG GTCCCTGCAG 
CTTATCTTGG TTTCCACCCC AGCTCTCCTC GTGGCAATGC ACGTAGCTCA CCAACAGCAC 
ATAGAAAAGA AAATGCTACG GCTTGAGGGG CATGGGGACC CCCTTCACCT GGAAGAGGTA 
AAGAGACACA AGGTGCACAT CTCAGGGACA CTGTGGTGGA CCTATGTCAT CAGTGTGGTG 
TTCCGGCTGC TGTTCGAGGC TGTCTTCATG TATGTCTTCT ATCTGCTCTA CCCCGGCTAT 
GCCATGGTGC GGCTGGTCAA GTGTGAAGCC TTCCCCTGCC CCAACACAGT GGACTGCTTC 
GTGTCCCGCC CCACCGAGAA AACCGTCTTC ACTGTCTTTA TGCTCGCAGC CTCCGGCATC 
TGCATTATCC TCAACGTGGC GGAGGTGGTG TACCTCATCA TCCGGGCCTG TGCCCGCCGT 
GCTCAGCGCC GCTCCAATCC GCCCTCCCGC AAGGGCTCGG GCTTCGGCCA CCGCCTCTCA 
CCTGAATACA AGCAGAATGA GATCAACAAG CTGCTGAGCG AGCAGGATGG CTCTCTGAAA 
GACATACTGC GCCGCAGCCC TGGCACAGGG GCCGGGCTCG CTGAAAAGAG CGACCGATGC 
TCAGCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gjb1 ( NM_001302497.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289426.1 Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu01388
Clone ID Related Accession (Same CDS sequence)	NM_001302498.1 , XM_030251227.1 , NM_001302496.1 , NM_008124.3 , NM_001302497.1
Accession Version	NM_001302497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	gap junction beta-1 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL683892.12, BE862747.1, BY087537.1, AK134742.1, AA423068.1 and AI849881.1. On Oct 24, 2014 this sequence version replaced XM_006527825.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All of the variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI851503.1, BI555945.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGGA CAGGTCTATA CACCTTGCTC AGTGGCGTGA ATCGGCACTC TACAGCCATT 
GGCCGAGTAT GGCTGTCTGT CATCTTCATC TTCAGAATCA TGGTGCTGGT GGTGGCTGCT 
GAGAGCGTGT GGGGGGATGA GAAGTCCTCT TTCATCTGTA ACACCCTCCA GCCGGGCTGC 
AACAGCGTCT GCTATGACCA TTTTTTCCCC ATCTCCCACG TGCGCCTATG GTCCCTGCAG 
CTTATCTTGG TTTCCACCCC AGCTCTCCTC GTGGCAATGC ACGTAGCTCA CCAACAGCAC 
ATAGAAAAGA AAATGCTACG GCTTGAGGGG CATGGGGACC CCCTTCACCT GGAAGAGGTA 
AAGAGACACA AGGTGCACAT CTCAGGGACA CTGTGGTGGA CCTATGTCAT CAGTGTGGTG 
TTCCGGCTGC TGTTCGAGGC TGTCTTCATG TATGTCTTCT ATCTGCTCTA CCCCGGCTAT 
GCCATGGTGC GGCTGGTCAA GTGTGAAGCC TTCCCCTGCC CCAACACAGT GGACTGCTTC 
GTGTCCCGCC CCACCGAGAA AACCGTCTTC ACTGTCTTTA TGCTCGCAGC CTCCGGCATC 
TGCATTATCC TCAACGTGGC GGAGGTGGTG TACCTCATCA TCCGGGCCTG TGCCCGCCGT 
GCTCAGCGCC GCTCCAATCC GCCCTCCCGC AAGGGCTCGG GCTTCGGCCA CCGCCTCTCA 
CCTGAATACA AGCAGAATGA GATCAACAAG CTGCTGAGCG AGCAGGATGG CTCTCTGAAA 
GACATACTGC GCCGCAGCCC TGGCACAGGG GCCGGGCTCG CTGAAAAGAG CGACCGATGC 
TCAGCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289426.1
CDS	128..979
Translation	MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDHFFPISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCEAFPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC

Target ORF information:

RefSeq Version	NM_001302497.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302497.1

ORF Insert Sequence:

ATGAACTGGA CAGGTCTATA CACCTTGCTC AGTGGCGTGA ATCGGCACTC TACAGCCATT 
GGCCGAGTAT GGCTGTCTGT CATCTTCATC TTCAGAATCA TGGTGCTGGT GGTGGCTGCT 
GAGAGCGTGT GGGGGGATGA GAAGTCCTCT TTCATCTGTA ACACCCTCCA GCCGGGCTGC 
AACAGCGTCT GCTATGACCA TTTTTTCCCC ATCTCCCACG TGCGCCTATG GTCCCTGCAG 
CTTATCTTGG TTTCCACCCC AGCTCTCCTC GTGGCAATGC ACGTAGCTCA CCAACAGCAC 
ATAGAAAAGA AAATGCTACG GCTTGAGGGG CATGGGGACC CCCTTCACCT GGAAGAGGTA 
AAGAGACACA AGGTGCACAT CTCAGGGACA CTGTGGTGGA CCTATGTCAT CAGTGTGGTG 
TTCCGGCTGC TGTTCGAGGC TGTCTTCATG TATGTCTTCT ATCTGCTCTA CCCCGGCTAT 
GCCATGGTGC GGCTGGTCAA GTGTGAAGCC TTCCCCTGCC CCAACACAGT GGACTGCTTC 
GTGTCCCGCC CCACCGAGAA AACCGTCTTC ACTGTCTTTA TGCTCGCAGC CTCCGGCATC 
TGCATTATCC TCAACGTGGC GGAGGTGGTG TACCTCATCA TCCGGGCCTG TGCCCGCCGT 
GCTCAGCGCC GCTCCAATCC GCCCTCCCGC AAGGGCTCGG GCTTCGGCCA CCGCCTCTCA 
CCTGAATACA AGCAGAATGA GATCAACAAG CTGCTGAGCG AGCAGGATGG CTCTCTGAAA 
GACATACTGC GCCGCAGCCC TGGCACAGGG GCCGGGCTCG CTGAAAAGAG CGACCGATGC 
TCAGCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gjb1 ( XM_030251227.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107087.1 Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant X1, mRNA.

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CloneID	OMu01388
Clone ID Related Accession (Same CDS sequence)	NM_001302498.1 , XM_030251227.1 , NM_001302496.1 , NM_008124.3 , NM_001302497.1
Accession Version	XM_030251227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	gap junction beta-1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTGGA CAGGTCTATA CACCTTGCTC AGTGGCGTGA ATCGGCACTC TACAGCCATT 
GGCCGAGTAT GGCTGTCTGT CATCTTCATC TTCAGAATCA TGGTGCTGGT GGTGGCTGCT 
GAGAGCGTGT GGGGGGATGA GAAGTCCTCT TTCATCTGTA ACACCCTCCA GCCGGGCTGC 
AACAGCGTCT GCTATGACCA TTTTTTCCCC ATCTCCCACG TGCGCCTATG GTCCCTGCAG 
CTTATCTTGG TTTCCACCCC AGCTCTCCTC GTGGCAATGC ACGTAGCTCA CCAACAGCAC 
ATAGAAAAGA AAATGCTACG GCTTGAGGGG CATGGGGACC CCCTTCACCT GGAAGAGGTA 
AAGAGACACA AGGTGCACAT CTCAGGGACA CTGTGGTGGA CCTATGTCAT CAGTGTGGTG 
TTCCGGCTGC TGTTCGAGGC TGTCTTCATG TATGTCTTCT ATCTGCTCTA CCCCGGCTAT 
GCCATGGTGC GGCTGGTCAA GTGTGAAGCC TTCCCCTGCC CCAACACAGT GGACTGCTTC 
GTGTCCCGCC CCACCGAGAA AACCGTCTTC ACTGTCTTTA TGCTCGCAGC CTCCGGCATC 
TGCATTATCC TCAACGTGGC GGAGGTGGTG TACCTCATCA TCCGGGCCTG TGCCCGCCGT 
GCTCAGCGCC GCTCCAATCC GCCCTCCCGC AAGGGCTCGG GCTTCGGCCA CCGCCTCTCA 
CCTGAATACA AGCAGAATGA GATCAACAAG CTGCTGAGCG AGCAGGATGG CTCTCTGAAA 
GACATACTGC GCCGCAGCCC TGGCACAGGG GCCGGGCTCG CTGAAAAGAG CGACCGATGC 
TCAGCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107087.1
CDS	443..1294
Translation	MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDHFFPISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCEAFPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC

Target ORF information:

RefSeq Version	XM_030251227.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, beta 1 (Gjb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251227.1

ORF Insert Sequence:

ATGAACTGGA CAGGTCTATA CACCTTGCTC AGTGGCGTGA ATCGGCACTC TACAGCCATT 
GGCCGAGTAT GGCTGTCTGT CATCTTCATC TTCAGAATCA TGGTGCTGGT GGTGGCTGCT 
GAGAGCGTGT GGGGGGATGA GAAGTCCTCT TTCATCTGTA ACACCCTCCA GCCGGGCTGC 
AACAGCGTCT GCTATGACCA TTTTTTCCCC ATCTCCCACG TGCGCCTATG GTCCCTGCAG 
CTTATCTTGG TTTCCACCCC AGCTCTCCTC GTGGCAATGC ACGTAGCTCA CCAACAGCAC 
ATAGAAAAGA AAATGCTACG GCTTGAGGGG CATGGGGACC CCCTTCACCT GGAAGAGGTA 
AAGAGACACA AGGTGCACAT CTCAGGGACA CTGTGGTGGA CCTATGTCAT CAGTGTGGTG 
TTCCGGCTGC TGTTCGAGGC TGTCTTCATG TATGTCTTCT ATCTGCTCTA CCCCGGCTAT 
GCCATGGTGC GGCTGGTCAA GTGTGAAGCC TTCCCCTGCC CCAACACAGT GGACTGCTTC 
GTGTCCCGCC CCACCGAGAA AACCGTCTTC ACTGTCTTTA TGCTCGCAGC CTCCGGCATC 
TGCATTATCC TCAACGTGGC GGAGGTGGTG TACCTCATCA TCCGGGCCTG TGCCCGCCGT 
GCTCAGCGCC GCTCCAATCC GCCCTCCCGC AAGGGCTCGG GCTTCGGCCA CCGCCTCTCA 
CCTGAATACA AGCAGAATGA GATCAACAAG CTGCTGAGCG AGCAGGATGG CTCTCTGAAA 
GACATACTGC GCCGCAGCCC TGGCACAGGG GCCGGGCTCG CTGAAAAGAG CGACCGATGC 
TCAGCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Connexin43, but not connexin30, contributes to adult neurogenesis in the dentate gyrus.
Brain research bulletin13691-100(2018 Jan)
Zhang J,Griemsmann S,Wu Z,Dobrowolski R,Willecke K,Theis M,Steinh?user C,Bedner P

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Gjb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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