Gpr83 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr83
Entrez Gene ID	14608
Full Name	G protein-coupled receptor 83
Synonyms	AW045253,Gir,Gpr72,RP105,RP39,RP82
General protein information	Preferred Names G protein-coupled receptor 83 Names probable G-protein coupled receptor 83 glucocorticoid-induced receptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 A2\|9 4.4 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006510017.3	XP_006510080.1	probable G-protein coupled receptor 83 isoform X2
XM_006510017.2	XP_006510080.1	probable G-protein coupled receptor 83 isoform X1
XM_030244058.1	XP_030099918.1	probable G-protein coupled receptor 83 isoform X1
NM_010287.3	NP_034417.1	probable G-protein coupled receptor 83 isoform 1 precursor
NM_001310734.1	NP_001297663.1	probable G-protein coupled receptor 83 isoform 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GPR83	NP_001003108.1
Homo sapiens (human)	GPR83	NP_057624.3
	GPR83	XP_001088588.1
Gallus gallus (chicken)	GPR83	NP_001243067.1
Pan troglodytes (chimpanzee)	GPR83	XP_522151.2
Mus musculus (house mouse)	Gpr83	NP_034417.1
Xenopus tropicalis (tropical clawed frog)	LOC100486806	XP_002939760.1
Rattus norvegicus (Norway rat)	Gpr83	NP_536336.1

Gene Ontology
Bibliography

Related articles in PubMed

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
Badgandi HB, Hwang SH, Shimada IS, Loriot E, Mukhopadhyay S
The Journal of cell biology216(3)743-760(2017 Mar)

Identification of GPR83 as the receptor for the neuroendocrine peptide PEN.
Gomes I, Bobeck EN, Margolis EB, Gupta A, Sierra S, Fakira AK, Fujita W, M?ller TD, M?ller A, Tsch?p MH, Kleinau G, Fricker LD, Devi LA
Science signaling9(425)ra43(2016 Apr)

The extracellular N-terminal domain of G-protein coupled receptor 83 regulates signaling properties and is an intramolecular inverse agonist.
M?ller A, Leinweber B, Fischer J, M?ller TD, Gr?ters A, Tsch?p MH, Kn?uper V, Biebermann H, Kleinau G
BMC research notes7913(2014 Dec)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Neuropeptide Y family receptors traffic via the Bardet-Biedl syndrome pathway to signal in neuronal primary cilia.
Loktev AV, Jackson PK
Cell reports5(5)1316-29(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study identified a receptor for PEN in mouse hypothalamus and Neuro2A cellsit. It is GPR83.
Title: Identification of GPR83 as the receptor for the neuroendocrine peptide PEN.

Data indicate that the extracellular portion of G-protein coupled receptor 83 (GPR83) has a strong regulatory function on this receptor.
Title: The extracellular N-terminal domain of G-protein coupled receptor 83 regulates signaling properties and is an intramolecular inverse agonist.

GIR -/- mice show increased preference for sucrose, delayed acquisition of spatial learning and insensitivity to the anxiogenic effects of restraint stress.
Title: Attenuated stress-evoked anxiety, increased sucrose preference and delayed spatial learning in glucocorticoid-induced receptor-deficient mice.

Gpr83 modulates ghrelin action and Gpr83 regulates systemic metabolism through other ghrelin-independent pathways.
Title: The orphan receptor Gpr83 regulates systemic energy metabolism via ghrelin-dependent and ghrelin-independent mechanisms.

GPR83 may participate in central thermoregulation and the central control of circulating adiponectin.
Title: Downregulation of GPR83 in the hypothalamic preoptic area reduces core body temperature and elevates circulating levels of adiponectin.

The following Gpr83 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr83 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67828	NM_001310734.1 Latest version!	Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu14833	NM_010287.3 Latest version!	Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu42008	XM_006510017.3 Latest version!	Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu90059	XM_030244058.1 Latest version!	Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu42008	XM_006510017.2	Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr83 ( NM_001310734.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297663.1 Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67828
Clone ID Related Accession (Same CDS sequence)	NM_001310734.1
Accession Version	NM_001310734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 83 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK035356.1, AK041527.1, AC136743.5 and AK030359.1. On Jul 18, 2015 this sequence version replaced XM_006510018.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK041527.1, SRR1660819.244562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTTC CTCCTGTCCT GCTTCTCTTT CTTCTGTCCT CAGTGCGAGC TACTGAGCAA 
CCGCAGGTCG TCACTGAGCA TCCCAGCATG GAGGCAGCCC TGACCGGGCC CAACGCCTCC 
TCGCACTTCT GGGCCAACTA CACTTTCTCT GACTGGCAGA ACTTCGTGGG CAGGAGACGT 
TATGGGGCCG AGTCCCAGAA CCCCACGGTG AAAGCACTGC TCATCGTGGC CTACTCATTC 
ACCATCGTCT TCTCGCTCTT CGGTAATGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGCATGCACT CGGCCACCAG CCTCTTCATT GTCAACCTGG CAGTGGCGGA CATCATGATC 
ACATTGCTCA ACACGCCCTT CACTTTGGTC ATCATGCATC CACTGAAGCC TCGGATCTCC 
ATCACCAAGG GTGTCATATA TATTGCTGTC ATCTGGGTCA TGGCTACCTT CTTCTCTCTG 
CCACATGCCA TCTGCCAGAA ACTGTTTACC TTCAAGTACA GTGAGGACAT TGTGCGCTCC 
CTCTGCCTGC CGGACTTCCC GGAGCCAGCT GACCTCTTCT GGAAGTATCT GGACCTGGCC 
ACCTTCATCC TGCTCTACCT ACTTCCACTC TTCATTATCT CAGTGGCCTA TGCTCGTGTG 
GCCAAGAAGC TGTGGCTCTG TAACACCATT GGCGACGTGA CCACAGAGCA GTACCTCGCC 
CTGCGACGCA AGAAGAAGAC CACCGTGAAG ATGCTGGTGC TTGTGGTAGT CCTCTTTGCC 
CTCTGCTGGT TCCCTCTCAA CTGCTATGTC CTCCTCTTGT CCAGCAAGGC CATCCACACC 
AACAATGCCC TCTACTTTGC CTTCCACTGG TTTGCCATGA GCAGTACTTG TTATAACCCC 
TTCATCTACT GCTGGCTCAA TGAGAACTTT AGGGTTGAGC TTAAGGCATT GCTGAGCATG 
TGCCAAAGGC CACCCAAGCC GCAGGAAGAC AGGCTACCCT CCCCAGTTCC TTCCTTCAGG 
GTGGCATGGA CAGAGAAGAG CCATGGTCGG AGGGCTCCAC TACCTAATCA CCACTTGCCC 
TCTTCCCAGA TCCAGTCTGG GAAGACAGAT CTGTCATCTG TGGAACCCGT TGTGGCCATG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297663.1
CDS	325..1470
Translation	MKVPPVLLLFLLSSVRATEQPQVVTEHPSMEAALTGPNASSHFWANYTFSDWQNFVGRRRYGAESQNPTVKALLIVAYSFTIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMITLLNTPFTLVIMHPLKPRISITKGVIYIAVIWVMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLDLATFILLYLLPLFIISVAYARVAKKLWLCNTIGDVTTEQYLALRRKKKTTVKMLVLVVVLFALCWFPLNCYVLLLSSKAIHTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRVELKALLSMCQRPPKPQEDRLPSPVPSFRVAWTEKSHGRRAPLPNHHLPSSQIQSGKTDLSSVEPVVAMS

Target ORF information:

RefSeq Version	NM_001310734.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310734.1

ORF Insert Sequence:

ATGAAGGTTC CTCCTGTCCT GCTTCTCTTT CTTCTGTCCT CAGTGCGAGC TACTGAGCAA 
CCGCAGGTCG TCACTGAGCA TCCCAGCATG GAGGCAGCCC TGACCGGGCC CAACGCCTCC 
TCGCACTTCT GGGCCAACTA CACTTTCTCT GACTGGCAGA ACTTCGTGGG CAGGAGACGT 
TATGGGGCCG AGTCCCAGAA CCCCACGGTG AAAGCACTGC TCATCGTGGC CTACTCATTC 
ACCATCGTCT TCTCGCTCTT CGGTAATGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGCATGCACT CGGCCACCAG CCTCTTCATT GTCAACCTGG CAGTGGCGGA CATCATGATC 
ACATTGCTCA ACACGCCCTT CACTTTGGTC ATCATGCATC CACTGAAGCC TCGGATCTCC 
ATCACCAAGG GTGTCATATA TATTGCTGTC ATCTGGGTCA TGGCTACCTT CTTCTCTCTG 
CCACATGCCA TCTGCCAGAA ACTGTTTACC TTCAAGTACA GTGAGGACAT TGTGCGCTCC 
CTCTGCCTGC CGGACTTCCC GGAGCCAGCT GACCTCTTCT GGAAGTATCT GGACCTGGCC 
ACCTTCATCC TGCTCTACCT ACTTCCACTC TTCATTATCT CAGTGGCCTA TGCTCGTGTG 
GCCAAGAAGC TGTGGCTCTG TAACACCATT GGCGACGTGA CCACAGAGCA GTACCTCGCC 
CTGCGACGCA AGAAGAAGAC CACCGTGAAG ATGCTGGTGC TTGTGGTAGT CCTCTTTGCC 
CTCTGCTGGT TCCCTCTCAA CTGCTATGTC CTCCTCTTGT CCAGCAAGGC CATCCACACC 
AACAATGCCC TCTACTTTGC CTTCCACTGG TTTGCCATGA GCAGTACTTG TTATAACCCC 
TTCATCTACT GCTGGCTCAA TGAGAACTTT AGGGTTGAGC TTAAGGCATT GCTGAGCATG 
TGCCAAAGGC CACCCAAGCC GCAGGAAGAC AGGCTACCCT CCCCAGTTCC TTCCTTCAGG 
GTGGCATGGA CAGAGAAGAG CCATGGTCGG AGGGCTCCAC TACCTAATCA CCACTTGCCC 
TCTTCCCAGA TCCAGTCTGG GAAGACAGAT CTGTCATCTG TGGAACCCGT TGTGGCCATG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr83 ( XM_006510017.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510080.1 Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42008
Clone ID Related Accession (Same CDS sequence)	XM_006510017.3 , XM_006510017.2
Accession Version	XM_006510017.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 83 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006510017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTC CTCCTGTCCT GCTTCTCTTT CTTCTGTCCT CAGTGCGAGC TACTGAGCAA 
CCGCAGGTCG TCACTGAGCA TCCCAGCATG GAGGCAGCCC TGACCGGGCC CAACGCCTCC 
TCGCACTTCT GGGCCAACTA CACTTTCTCT GACTGGCAGA ACTTCGTGGG CAGGAGACGT 
TATGGGGCCG AGTCCCAGAA CCCCACGGTG AAAGCACTGC TCATCGTGGC CTACTCATTC 
ACCATCGTCT TCTCGCTCTT CGGTAATGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGCATGCACT CGGCCACCAG CCTCTTCATT GTCAACCTGG CAGTGGCGGA CATCATGATC 
ACATTGCTCA ACACGCCCTT CACTTTGGTC CGCTTTGTGA ACAGCACATG GGTGTTTGGG 
AAGGGCATGT GTCATGTCAG TCGCTTTGCT CAGTACTGTT CTCTACATGT CTCAGCACTG 
ACTCTGACAG CTATCGCAGT GGACCGCCAC CAGAGGCCTT GGGACTTCCA GGAGTCACAG 
AGTCTACATG ACACCCTCTT CCCACCCCTG GAGGTCATCA TGCATCCACT GAAGCCTCGG 
ATCTCCATCA CCAAGGGTGT CATATATATT GCTGTCATCT GGGTCATGGC TACCTTCTTC 
TCTCTGCCAC ATGCCATCTG CCAGAAACTG TTTACCTTCA AGTACAGTGA GGACATTGTG 
CGCTCCCTCT GCCTGCCGGA CTTCCCGGAG CCAGCTGACC TCTTCTGGAA GTATCTGGAC 
CTGGCCACCT TCATCCTGCT CTACCTACTT CCACTCTTCA TTATCTCAGT GGCCTATGCT 
CGTGTGGCCA AGAAGCTGTG GCTCTGTAAC ACCATTGGCG ACGTGACCAC AGAGCAGTAC 
CTCGCCCTGC GACGCAAGAA GAAGACCACC GTGAAGATGC TGGTGCTTGT GGTAGTCCTC 
TTTGCCCTCT GCTGGTTCCC TCTCAACTGC TATGTCCTCC TCTTGTCCAG CAAGGCCATC 
CACACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG TACTTGTTAT 
AACCCCTTCA TCTACTGCTG GCTCAATGAG AACTTTAGGG TTGAGCTTAA GGCATTGCTG 
AGCATGTGCC AAAGGCCACC CAAGCCGCAG GAAGACAGGC TACCCTCCCC AGTTCCTTCC 
TTCAGGGTGG CATGGACAGA GAAGAGCCAT GGTCGGAGGG CTCCACTACC TAATCACCAC 
TTGCCCTCTT CCCAGATCCA GTCTGGGAAG ACAGATCTGT CATCTGTGGA ACCCGTTGTG 
GCCATGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510080.1
CDS	732..2063
Translation	MKVPPVLLLFLLSSVRATEQPQVVTEHPSMEAALTGPNASSHFWANYTFSDWQNFVGRRRYGAESQNPTVKALLIVAYSFTIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMITLLNTPFTLVRFVNSTWVFGKGMCHVSRFAQYCSLHVSALTLTAIAVDRHQRPWDFQESQSLHDTLFPPLEVIMHPLKPRISITKGVIYIAVIWVMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLDLATFILLYLLPLFIISVAYARVAKKLWLCNTIGDVTTEQYLALRRKKKTTVKMLVLVVVLFALCWFPLNCYVLLLSSKAIHTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRVELKALLSMCQRPPKPQEDRLPSPVPSFRVAWTEKSHGRRAPLPNHHLPSSQIQSGKTDLSSVEPVVAMS

Target ORF information:

RefSeq Version	XM_006510017.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510017.2

ORF Insert Sequence:

ATGAAGGTTC CTCCTGTCCT GCTTCTCTTT CTTCTGTCCT CAGTGCGAGC TACTGAGCAA 
CCGCAGGTCG TCACTGAGCA TCCCAGCATG GAGGCAGCCC TGACCGGGCC CAACGCCTCC 
TCGCACTTCT GGGCCAACTA CACTTTCTCT GACTGGCAGA ACTTCGTGGG CAGGAGACGT 
TATGGGGCCG AGTCCCAGAA CCCCACGGTG AAAGCACTGC TCATCGTGGC CTACTCATTC 
ACCATCGTCT TCTCGCTCTT CGGTAATGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGCATGCACT CGGCCACCAG CCTCTTCATT GTCAACCTGG CAGTGGCGGA CATCATGATC 
ACATTGCTCA ACACGCCCTT CACTTTGGTC CGCTTTGTGA ACAGCACATG GGTGTTTGGG 
AAGGGCATGT GTCATGTCAG TCGCTTTGCT CAGTACTGTT CTCTACATGT CTCAGCACTG 
ACTCTGACAG CTATCGCAGT GGACCGCCAC CAGAGGCCTT GGGACTTCCA GGAGTCACAG 
AGTCTACATG ACACCCTCTT CCCACCCCTG GAGGTCATCA TGCATCCACT GAAGCCTCGG 
ATCTCCATCA CCAAGGGTGT CATATATATT GCTGTCATCT GGGTCATGGC TACCTTCTTC 
TCTCTGCCAC ATGCCATCTG CCAGAAACTG TTTACCTTCA AGTACAGTGA GGACATTGTG 
CGCTCCCTCT GCCTGCCGGA CTTCCCGGAG CCAGCTGACC TCTTCTGGAA GTATCTGGAC 
CTGGCCACCT TCATCCTGCT CTACCTACTT CCACTCTTCA TTATCTCAGT GGCCTATGCT 
CGTGTGGCCA AGAAGCTGTG GCTCTGTAAC ACCATTGGCG ACGTGACCAC AGAGCAGTAC 
CTCGCCCTGC GACGCAAGAA GAAGACCACC GTGAAGATGC TGGTGCTTGT GGTAGTCCTC 
TTTGCCCTCT GCTGGTTCCC TCTCAACTGC TATGTCCTCC TCTTGTCCAG CAAGGCCATC 
CACACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG TACTTGTTAT 
AACCCCTTCA TCTACTGCTG GCTCAATGAG AACTTTAGGG TTGAGCTTAA GGCATTGCTG 
AGCATGTGCC AAAGGCCACC CAAGCCGCAG GAAGACAGGC TACCCTCCCC AGTTCCTTCC 
TTCAGGGTGG CATGGACAGA GAAGAGCCAT GGTCGGAGGG CTCCACTACC TAATCACCAC 
TTGCCCTCTT CCCAGATCCA GTCTGGGAAG ACAGATCTGT CATCTGTGGA ACCCGTTGTG 
GCCATGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr83 ( NM_010287.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034417.1 Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14833
Clone ID Related Accession (Same CDS sequence)	NM_010287.3
Accession Version	NM_010287.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 83 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK035356.1, AK081276.1, AK041527.1 and AK030359.1. On Jul 18, 2015 this sequence version replaced NM_010287.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK081276.1, AK035356.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTTC CTCCTGTCCT GCTTCTCTTT CTTCTGTCCT CAGTGCGAGC TACTGAGCAA 
CCGCAGGTCG TCACTGAGCA TCCCAGCATG GAGGCAGCCC TGACCGGGCC CAACGCCTCC 
TCGCACTTCT GGGCCAACTA CACTTTCTCT GACTGGCAGA ACTTCGTGGG CAGGAGACGT 
TATGGGGCCG AGTCCCAGAA CCCCACGGTG AAAGCACTGC TCATCGTGGC CTACTCATTC 
ACCATCGTCT TCTCGCTCTT CGGTAATGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGCATGCACT CGGCCACCAG CCTCTTCATT GTCAACCTGG CAGTGGCGGA CATCATGATC 
ACATTGCTCA ACACGCCCTT CACTTTGGTC CGCTTTGTGA ACAGCACATG GGTGTTTGGG 
AAGGGCATGT GTCATGTCAG TCGCTTTGCT CAGTACTGTT CTCTACATGT CTCAGCACTG 
ACTCTGACAG CTATCGCAGT GGACCGCCAC CAGGTCATCA TGCATCCACT GAAGCCTCGG 
ATCTCCATCA CCAAGGGTGT CATATATATT GCTGTCATCT GGGTCATGGC TACCTTCTTC 
TCTCTGCCAC ATGCCATCTG CCAGAAACTG TTTACCTTCA AGTACAGTGA GGACATTGTG 
CGCTCCCTCT GCCTGCCGGA CTTCCCGGAG CCAGCTGACC TCTTCTGGAA GTATCTGGAC 
CTGGCCACCT TCATCCTGCT CTACCTACTT CCACTCTTCA TTATCTCAGT GGCCTATGCT 
CGTGTGGCCA AGAAGCTGTG GCTCTGTAAC ACCATTGGCG ACGTGACCAC AGAGCAGTAC 
CTCGCCCTGC GACGCAAGAA GAAGACCACC GTGAAGATGC TGGTGCTTGT GGTAGTCCTC 
TTTGCCCTCT GCTGGTTCCC TCTCAACTGC TATGTCCTCC TCTTGTCCAG CAAGGCCATC 
CACACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG TACTTGTTAT 
AACCCCTTCA TCTACTGCTG GCTCAATGAG AACTTTAGGG TTGAGCTTAA GGCATTGCTG 
AGCATGTGCC AAAGGCCACC CAAGCCGCAG GAAGACAGGC TACCCTCCCC AGTTCCTTCC 
TTCAGGGTGG CATGGACAGA GAAGAGCCAT GGTCGGAGGG CTCCACTACC TAATCACCAC 
TTGCCCTCTT CCCAGATCCA GTCTGGGAAG ACAGATCTGT CATCTGTGGA ACCCGTTGTG 
GCCATGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034417.1
CDS	325..1596
Translation	MKVPPVLLLFLLSSVRATEQPQVVTEHPSMEAALTGPNASSHFWANYTFSDWQNFVGRRRYGAESQNPTVKALLIVAYSFTIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMITLLNTPFTLVRFVNSTWVFGKGMCHVSRFAQYCSLHVSALTLTAIAVDRHQVIMHPLKPRISITKGVIYIAVIWVMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLDLATFILLYLLPLFIISVAYARVAKKLWLCNTIGDVTTEQYLALRRKKKTTVKMLVLVVVLFALCWFPLNCYVLLLSSKAIHTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRVELKALLSMCQRPPKPQEDRLPSPVPSFRVAWTEKSHGRRAPLPNHHLPSSQIQSGKTDLSSVEPVVAMS

Target ORF information:

RefSeq Version	NM_010287.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010287.3

ORF Insert Sequence:

ATGAAGGTTC CTCCTGTCCT GCTTCTCTTT CTTCTGTCCT CAGTGCGAGC TACTGAGCAA 
CCGCAGGTCG TCACTGAGCA TCCCAGCATG GAGGCAGCCC TGACCGGGCC CAACGCCTCC 
TCGCACTTCT GGGCCAACTA CACTTTCTCT GACTGGCAGA ACTTCGTGGG CAGGAGACGT 
TATGGGGCCG AGTCCCAGAA CCCCACGGTG AAAGCACTGC TCATCGTGGC CTACTCATTC 
ACCATCGTCT TCTCGCTCTT CGGTAATGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGCATGCACT CGGCCACCAG CCTCTTCATT GTCAACCTGG CAGTGGCGGA CATCATGATC 
ACATTGCTCA ACACGCCCTT CACTTTGGTC CGCTTTGTGA ACAGCACATG GGTGTTTGGG 
AAGGGCATGT GTCATGTCAG TCGCTTTGCT CAGTACTGTT CTCTACATGT CTCAGCACTG 
ACTCTGACAG CTATCGCAGT GGACCGCCAC CAGGTCATCA TGCATCCACT GAAGCCTCGG 
ATCTCCATCA CCAAGGGTGT CATATATATT GCTGTCATCT GGGTCATGGC TACCTTCTTC 
TCTCTGCCAC ATGCCATCTG CCAGAAACTG TTTACCTTCA AGTACAGTGA GGACATTGTG 
CGCTCCCTCT GCCTGCCGGA CTTCCCGGAG CCAGCTGACC TCTTCTGGAA GTATCTGGAC 
CTGGCCACCT TCATCCTGCT CTACCTACTT CCACTCTTCA TTATCTCAGT GGCCTATGCT 
CGTGTGGCCA AGAAGCTGTG GCTCTGTAAC ACCATTGGCG ACGTGACCAC AGAGCAGTAC 
CTCGCCCTGC GACGCAAGAA GAAGACCACC GTGAAGATGC TGGTGCTTGT GGTAGTCCTC 
TTTGCCCTCT GCTGGTTCCC TCTCAACTGC TATGTCCTCC TCTTGTCCAG CAAGGCCATC 
CACACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG TACTTGTTAT 
AACCCCTTCA TCTACTGCTG GCTCAATGAG AACTTTAGGG TTGAGCTTAA GGCATTGCTG 
AGCATGTGCC AAAGGCCACC CAAGCCGCAG GAAGACAGGC TACCCTCCCC AGTTCCTTCC 
TTCAGGGTGG CATGGACAGA GAAGAGCCAT GGTCGGAGGG CTCCACTACC TAATCACCAC 
TTGCCCTCTT CCCAGATCCA GTCTGGGAAG ACAGATCTGT CATCTGTGGA ACCCGTTGTG 
GCCATGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr83 ( XM_006510017.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510080.1 Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant X2, mRNA.

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CloneID	OMu42008
Clone ID Related Accession (Same CDS sequence)	XM_006510017.3 , XM_006510017.2
Accession Version	XM_006510017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 83 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTC CTCCTGTCCT GCTTCTCTTT CTTCTGTCCT CAGTGCGAGC TACTGAGCAA 
CCGCAGGTCG TCACTGAGCA TCCCAGCATG GAGGCAGCCC TGACCGGGCC CAACGCCTCC 
TCGCACTTCT GGGCCAACTA CACTTTCTCT GACTGGCAGA ACTTCGTGGG CAGGAGACGT 
TATGGGGCCG AGTCCCAGAA CCCCACGGTG AAAGCACTGC TCATCGTGGC CTACTCATTC 
ACCATCGTCT TCTCGCTCTT CGGTAATGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGCATGCACT CGGCCACCAG CCTCTTCATT GTCAACCTGG CAGTGGCGGA CATCATGATC 
ACATTGCTCA ACACGCCCTT CACTTTGGTC CGCTTTGTGA ACAGCACATG GGTGTTTGGG 
AAGGGCATGT GTCATGTCAG TCGCTTTGCT CAGTACTGTT CTCTACATGT CTCAGCACTG 
ACTCTGACAG CTATCGCAGT GGACCGCCAC CAGAGGCCTT GGGACTTCCA GGAGTCACAG 
AGTCTACATG ACACCCTCTT CCCACCCCTG GAGGTCATCA TGCATCCACT GAAGCCTCGG 
ATCTCCATCA CCAAGGGTGT CATATATATT GCTGTCATCT GGGTCATGGC TACCTTCTTC 
TCTCTGCCAC ATGCCATCTG CCAGAAACTG TTTACCTTCA AGTACAGTGA GGACATTGTG 
CGCTCCCTCT GCCTGCCGGA CTTCCCGGAG CCAGCTGACC TCTTCTGGAA GTATCTGGAC 
CTGGCCACCT TCATCCTGCT CTACCTACTT CCACTCTTCA TTATCTCAGT GGCCTATGCT 
CGTGTGGCCA AGAAGCTGTG GCTCTGTAAC ACCATTGGCG ACGTGACCAC AGAGCAGTAC 
CTCGCCCTGC GACGCAAGAA GAAGACCACC GTGAAGATGC TGGTGCTTGT GGTAGTCCTC 
TTTGCCCTCT GCTGGTTCCC TCTCAACTGC TATGTCCTCC TCTTGTCCAG CAAGGCCATC 
CACACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG TACTTGTTAT 
AACCCCTTCA TCTACTGCTG GCTCAATGAG AACTTTAGGG TTGAGCTTAA GGCATTGCTG 
AGCATGTGCC AAAGGCCACC CAAGCCGCAG GAAGACAGGC TACCCTCCCC AGTTCCTTCC 
TTCAGGGTGG CATGGACAGA GAAGAGCCAT GGTCGGAGGG CTCCACTACC TAATCACCAC 
TTGCCCTCTT CCCAGATCCA GTCTGGGAAG ACAGATCTGT CATCTGTGGA ACCCGTTGTG 
GCCATGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510080.1
CDS	655..1986
Translation	MKVPPVLLLFLLSSVRATEQPQVVTEHPSMEAALTGPNASSHFWANYTFSDWQNFVGRRRYGAESQNPTVKALLIVAYSFTIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMITLLNTPFTLVRFVNSTWVFGKGMCHVSRFAQYCSLHVSALTLTAIAVDRHQRPWDFQESQSLHDTLFPPLEVIMHPLKPRISITKGVIYIAVIWVMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLDLATFILLYLLPLFIISVAYARVAKKLWLCNTIGDVTTEQYLALRRKKKTTVKMLVLVVVLFALCWFPLNCYVLLLSSKAIHTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRVELKALLSMCQRPPKPQEDRLPSPVPSFRVAWTEKSHGRRAPLPNHHLPSSQIQSGKTDLSSVEPVVAMS

Target ORF information:

RefSeq Version	XM_006510017.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510017.3

ORF Insert Sequence:

ATGAAGGTTC CTCCTGTCCT GCTTCTCTTT CTTCTGTCCT CAGTGCGAGC TACTGAGCAA 
CCGCAGGTCG TCACTGAGCA TCCCAGCATG GAGGCAGCCC TGACCGGGCC CAACGCCTCC 
TCGCACTTCT GGGCCAACTA CACTTTCTCT GACTGGCAGA ACTTCGTGGG CAGGAGACGT 
TATGGGGCCG AGTCCCAGAA CCCCACGGTG AAAGCACTGC TCATCGTGGC CTACTCATTC 
ACCATCGTCT TCTCGCTCTT CGGTAATGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGCATGCACT CGGCCACCAG CCTCTTCATT GTCAACCTGG CAGTGGCGGA CATCATGATC 
ACATTGCTCA ACACGCCCTT CACTTTGGTC CGCTTTGTGA ACAGCACATG GGTGTTTGGG 
AAGGGCATGT GTCATGTCAG TCGCTTTGCT CAGTACTGTT CTCTACATGT CTCAGCACTG 
ACTCTGACAG CTATCGCAGT GGACCGCCAC CAGAGGCCTT GGGACTTCCA GGAGTCACAG 
AGTCTACATG ACACCCTCTT CCCACCCCTG GAGGTCATCA TGCATCCACT GAAGCCTCGG 
ATCTCCATCA CCAAGGGTGT CATATATATT GCTGTCATCT GGGTCATGGC TACCTTCTTC 
TCTCTGCCAC ATGCCATCTG CCAGAAACTG TTTACCTTCA AGTACAGTGA GGACATTGTG 
CGCTCCCTCT GCCTGCCGGA CTTCCCGGAG CCAGCTGACC TCTTCTGGAA GTATCTGGAC 
CTGGCCACCT TCATCCTGCT CTACCTACTT CCACTCTTCA TTATCTCAGT GGCCTATGCT 
CGTGTGGCCA AGAAGCTGTG GCTCTGTAAC ACCATTGGCG ACGTGACCAC AGAGCAGTAC 
CTCGCCCTGC GACGCAAGAA GAAGACCACC GTGAAGATGC TGGTGCTTGT GGTAGTCCTC 
TTTGCCCTCT GCTGGTTCCC TCTCAACTGC TATGTCCTCC TCTTGTCCAG CAAGGCCATC 
CACACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG TACTTGTTAT 
AACCCCTTCA TCTACTGCTG GCTCAATGAG AACTTTAGGG TTGAGCTTAA GGCATTGCTG 
AGCATGTGCC AAAGGCCACC CAAGCCGCAG GAAGACAGGC TACCCTCCCC AGTTCCTTCC 
TTCAGGGTGG CATGGACAGA GAAGAGCCAT GGTCGGAGGG CTCCACTACC TAATCACCAC 
TTGCCCTCTT CCCAGATCCA GTCTGGGAAG ACAGATCTGT CATCTGTGGA ACCCGTTGTG 
GCCATGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr83 ( XM_030244058.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099918.1 Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu90059
Clone ID Related Accession (Same CDS sequence)	XM_030244058.1
Accession Version	XM_030244058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 83 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTC CTCCTGTCCT GCTTCTCTTT CTTCTGTCCT CAGTGCGAGC TACTGAGCAA 
CCGCAGGTCG TCACTGAGCA TCCCAGCATG GAGGCAGCCC TGACCGGGCC CAACGCCTCC 
TCGCACTTCT GGGCCAACTA CACTTTCTCT GACTGGCAGA ACTTCGTGGG CAGGAGACGT 
TATGGGGCCG AGTCCCAGAA CCCCACGGTG AAAGCACTGC TCATCGTGGC CTACTCATTC 
ACCATCGTCT TCTCGCTCTT CGGTAATGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGCATGCACT CGGCCACCAG CCTCTTCATT GTCAACCTGG CAGTGGCGGA CATCATGATC 
ACATTGCTCA ACACGCCCTT CACTTTGGTC CGCTTTGTGA ACAGCACATG GGTGTTTGGG 
AAGGGCATGT GTCATGTCAG TCGCTTTGCT CAGTACTGTT CTCTACATGT CTCAGCACTG 
ACTCTGACAG CTATCGCAGT GGACCGCCAC CAGGGTCTGG AACTACAGAA GATGGTCAGG 
CCTCGAGGAG ATGGGGGCGA GCTCAGGTCC CCTTCTGTCA CCTTTGTACC CTCCTCACTC 
TGCCCTGCTC TTTTCACTTG CAAGAGGCCT TGGGACTTCC AGGAGTCACA GAGTCTACAT 
GACACCCTCT TCCCACCCCT GGAGGTCATC ATGCATCCAC TGAAGCCTCG GATCTCCATC 
ACCAAGGGTG TCATATATAT TGCTGTCATC TGGGTCATGG CTACCTTCTT CTCTCTGCCA 
CATGCCATCT GCCAGAAACT GTTTACCTTC AAGTACAGTG AGGACATTGT GCGCTCCCTC 
TGCCTGCCGG ACTTCCCGGA GCCAGCTGAC CTCTTCTGGA AGTATCTGGA CCTGGCCACC 
TTCATCCTGC TCTACCTACT TCCACTCTTC ATTATCTCAG TGGCCTATGC TCGTGTGGCC 
AAGAAGCTGT GGCTCTGTAA CACCATTGGC GACGTGACCA CAGAGCAGTA CCTCGCCCTG 
CGACGCAAGA AGAAGACCAC CGTGAAGATG CTGGTGCTTG TGGTAGTCCT CTTTGCCCTC 
TGCTGGTTCC CTCTCAACTG CTATGTCCTC CTCTTGTCCA GCAAGGCCAT CCACACCAAC 
AATGCCCTCT ACTTTGCCTT CCACTGGTTT GCCATGAGCA GTACTTGTTA TAACCCCTTC 
ATCTACTGCT GGCTCAATGA GAACTTTAGG GTTGAGCTTA AGGCATTGCT GAGCATGTGC 
CAAAGGCCAC CCAAGCCGCA GGAAGACAGG CTACCCTCCC CAGTTCCTTC CTTCAGGGTG 
GCATGGACAG AGAAGAGCCA TGGTCGGAGG GCTCCACTAC CTAATCACCA CTTGCCCTCT 
TCCCAGATCC AGTCTGGGAA GACAGATCTG TCATCTGTGG AACCCGTTGT GGCCATGAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099918.1
CDS	655..2097
Translation	MKVPPVLLLFLLSSVRATEQPQVVTEHPSMEAALTGPNASSHFWANYTFSDWQNFVGRRRYGAESQNPTVKALLIVAYSFTIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMITLLNTPFTLVRFVNSTWVFGKGMCHVSRFAQYCSLHVSALTLTAIAVDRHQGLELQKMVRPRGDGGELRSPSVTFVPSSLCPALFTCKRPWDFQESQSLHDTLFPPLEVIMHPLKPRISITKGVIYIAVIWVMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLDLATFILLYLLPLFIISVAYARVAKKLWLCNTIGDVTTEQYLALRRKKKTTVKMLVLVVVLFALCWFPLNCYVLLLSSKAIHTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRVELKALLSMCQRPPKPQEDRLPSPVPSFRVAWTEKSHGRRAPLPNHHLPSSQIQSGKTDLSSVEPVVAMS

Target ORF information:

RefSeq Version	XM_030244058.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 83 (Gpr83), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244058.1

ORF Insert Sequence:

ATGAAGGTTC CTCCTGTCCT GCTTCTCTTT CTTCTGTCCT CAGTGCGAGC TACTGAGCAA 
CCGCAGGTCG TCACTGAGCA TCCCAGCATG GAGGCAGCCC TGACCGGGCC CAACGCCTCC 
TCGCACTTCT GGGCCAACTA CACTTTCTCT GACTGGCAGA ACTTCGTGGG CAGGAGACGT 
TATGGGGCCG AGTCCCAGAA CCCCACGGTG AAAGCACTGC TCATCGTGGC CTACTCATTC 
ACCATCGTCT TCTCGCTCTT CGGTAATGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGCATGCACT CGGCCACCAG CCTCTTCATT GTCAACCTGG CAGTGGCGGA CATCATGATC 
ACATTGCTCA ACACGCCCTT CACTTTGGTC CGCTTTGTGA ACAGCACATG GGTGTTTGGG 
AAGGGCATGT GTCATGTCAG TCGCTTTGCT CAGTACTGTT CTCTACATGT CTCAGCACTG 
ACTCTGACAG CTATCGCAGT GGACCGCCAC CAGGGTCTGG AACTACAGAA GATGGTCAGG 
CCTCGAGGAG ATGGGGGCGA GCTCAGGTCC CCTTCTGTCA CCTTTGTACC CTCCTCACTC 
TGCCCTGCTC TTTTCACTTG CAAGAGGCCT TGGGACTTCC AGGAGTCACA GAGTCTACAT 
GACACCCTCT TCCCACCCCT GGAGGTCATC ATGCATCCAC TGAAGCCTCG GATCTCCATC 
ACCAAGGGTG TCATATATAT TGCTGTCATC TGGGTCATGG CTACCTTCTT CTCTCTGCCA 
CATGCCATCT GCCAGAAACT GTTTACCTTC AAGTACAGTG AGGACATTGT GCGCTCCCTC 
TGCCTGCCGG ACTTCCCGGA GCCAGCTGAC CTCTTCTGGA AGTATCTGGA CCTGGCCACC 
TTCATCCTGC TCTACCTACT TCCACTCTTC ATTATCTCAG TGGCCTATGC TCGTGTGGCC 
AAGAAGCTGT GGCTCTGTAA CACCATTGGC GACGTGACCA CAGAGCAGTA CCTCGCCCTG 
CGACGCAAGA AGAAGACCAC CGTGAAGATG CTGGTGCTTG TGGTAGTCCT CTTTGCCCTC 
TGCTGGTTCC CTCTCAACTG CTATGTCCTC CTCTTGTCCA GCAAGGCCAT CCACACCAAC 
AATGCCCTCT ACTTTGCCTT CCACTGGTTT GCCATGAGCA GTACTTGTTA TAACCCCTTC 
ATCTACTGCT GGCTCAATGA GAACTTTAGG GTTGAGCTTA AGGCATTGCT GAGCATGTGC 
CAAAGGCCAC CCAAGCCGCA GGAAGACAGG CTACCCTCCC CAGTTCCTTC CTTCAGGGTG 
GCATGGACAG AGAAGAGCCA TGGTCGGAGG GCTCCACTAC CTAATCACCA CTTGCCCTCT 
TCCCAGATCC AGTCTGGGAA GACAGATCTG TCATCTGTGG AACCCGTTGT GGCCATGAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
The Journal of cell biology216(3)743-760(2017 Mar)
Badgandi HB,Hwang SH,Shimada IS,Loriot E,Mukhopadhyay S

Identification of GPR83 as the receptor for the neuroendocrine peptide PEN.
Science signaling9(425)ra43(2016 Apr)
Gomes I,Bobeck EN,Margolis EB,Gupta A,Sierra S,Fakira AK,Fujita W,M?ller TD,M?ller A,Tsch?p MH,Kleinau G,Fricker LD,Devi LA

The extracellular N-terminal domain of G-protein coupled receptor 83 regulates signaling properties and is an intramolecular inverse agonist.
BMC research notes7913(2014 Dec)
M?ller A,Leinweber B,Fischer J,M?ller TD,Gr?ters A,Tsch?p MH,Kn?uper V,Biebermann H,Kleinau G

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Neuropeptide Y family receptors traffic via the Bardet-Biedl syndrome pathway to signal in neuronal primary cilia.
Cell reports5(5)1316-29(2013 Dec)
Loktev AV,Jackson PK

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Gpr83 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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