Gfpt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gfpt1
Entrez Gene ID	14583
Full Name	glutamine fructose-6-phosphate transaminase 1
Synonyms	2810423A18Rik,AI324119,AI449986,GFA,GFAT,GFAT1,GFAT1m,Gfpt
General protein information	Preferred Names glutamine fructose-6-phosphate transaminase 1 Names glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 D-fructose-6-phosphate amidotransferase 1 GFAT 1 glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 glutamine:fructose-6-phosphate amidotransferase 1 hexosephosphate aminotransferase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 D1\|6 37.81 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013528.3	NP_038556.1	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gfpt1	NP_038556.1
Homo sapiens (human)	GFPT1	NP_001231639.1
	GFPT1	XP_001096250.2
Pan troglodytes (chimpanzee)	GFPT1	XP_003949855.1
Xenopus tropicalis (tropical clawed frog)	gfpt1	XP_002935349.1
Canis lupus familiaris (dog)	GFPT1	XP_005626234.1
Gallus gallus (chicken)	GFPT1	NP_001026053.1
Danio rerio (zebrafish)	gfpt1	NP_001030153.1
Bos taurus (cattle)	GFPT1	NP_001103431.1
Rattus norvegicus (Norway rat)	Gfpt1	NP_001005879.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

mTORC2 Responds to Glutamine Catabolite Levels to Modulate the Hexosamine Biosynthesis Enzyme GFAT1.
Moloughney JG, Kim PK, Vega-Cotto NM, Wu CC, Zhang S, Adlam M, Lynch T, Chou PC, Rabinowitz JD, Werlen G, Jacinto E
Molecular cell63(5)811-26(2016 Sep)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

O-GlcNAc signaling entrains the circadian clock by inhibiting BMAL1/CLOCK ubiquitination.
Li MD, Ruan HB, Hughes ME, Lee JS, Singh JP, Jones SP, Nitabach MN, Yang X
Cell metabolism17(2)303-10(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mTORC2 responds to glutamine catabolite levels to modulate the hexosamine biosynthesis enzyme GFAT1, and is essential for proper expression and nuclear accumulation of the GFAT1 transcriptional regulator, Xbp1s.
Title: mTORC2 Responds to Glutamine Catabolite Levels to Modulate the Hexosamine Biosynthesis Enzyme GFAT1.

GFAT-1 plays a critical role in modulating adipogenesis
Title: Suppression of Glutamine:fructose-6-phosphate amidotransferase-1 inhibits adipogenesis in 3T3-L1 adipocytes.

The following Gfpt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gfpt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21536	NM_013528.3 Latest version!	Mus musculus glutamine fructose-6-phosphate transaminase 1 (Gfpt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gfpt1 ( NM_013528.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038556.1 Mus musculus glutamine fructose-6-phosphate transaminase 1 (Gfpt1), mRNA.

Price & Availability↑

CloneID	OMu21536
Clone ID Related Accession (Same CDS sequence)	NM_013528.3
Accession Version	NM_013528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB664274.1, AK019852.1, AC162465.3 and CN664418.1. On Sep 19, 2008 this sequence version replaced NM_013528.2. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK019852.1, BC050762.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGTGCGGTA TATTTGCTTA TTTAAATTAC CATGTTCCTC GAACAAGACG AGAAATCTTG 
GAGACACTAA TCAAAGGCCT TCAGAGACTG GAATACAGAG GATATGATTC TGCTGGTGTG 
GGACTTGACG GAGGCAATGA CAAAGACTGG GAAGCCAACG CCTGCAAAAT CCAGCTCATT 
AAGAAGAAAG GAAAAGTTAA GGCACTGGAT GAAGAAGTTC ACAAACAACA AGATATGGAC 
TTGGATATAG AATTTGATGT GCATCTTGGA ATAGCTCATA CCCGTTGGGC GACACATGGA 
GAACCCAATC CTGTCAATAG TCACCCCCAG CGCTCTGATA AAAATAATGA ATTCATTGTT 
ATTCATAATG GAATCATCAC CAACTACAAA GACTTGAAAA AGTTTCTGGA AAGCAAAGGC 
TATGACTTTG AATCTGAAAC AGACACAGAA ACCATTGCCA AGCTCGTCAA GTACATGTAT 
GACAACTGGG AGAGCCAGGA CGTCAGTTTT ACCACCTTGG TGGAGAGAGT TATCCAACAA 
TTGGAAGGCG CCTTTGCTCT TGTGTTTAAA AGTGTCCATT TTCCCGGGCA AGCAGTTGGC 
ACAAGGCGAG GTAGCCCTCT CTTGATTGGT GTGCGGAGTG AACATAAGCT TTCTACAGAT 
CACATTCCGA TTCTGTACAG AACAGGCAAA GACAAGAAAG GAAGCTGCGG TCTTTCCCGT 
GTGGACAGCA CGACATGCCT GTTCCCTGTT GAGGAAAAGG CAGTTGAATA TTACTTTGCT 
TCTGATGCAA GTGCCGTGAT AGAGCACACC AATCGTGTCA TCTTTCTGGA AGATGATGAT 
GTTGCAGCAG TGGTGGATGG CCGTCTCTCT ATCCACCGAA TTAAACGAAC TGCAGGAGAC 
CATCCTGGCC GAGCTGTGCA AACTCTCCAG ATGGAGCTCC AGCAGATCAT GAAGGGCAAC 
TTTAGTTCAT TTATGCAGAA GGAAATTTTT GAGCAGCCAG AATCTGTTGT GAACACAATG 
AGAGGAAGAG TCAATTTTGA TGACTACACT GTGAATTTGG GAGGTTTGAA AGATCACATT 
AAGGAGATCC AGCGGTGTCG GCGGTTGATT CTTATTGCTT GTGGCACAAG TTACCACGCT 
GGTGTGGCAA CCCGTCAGGT CCTGGAGGAG CTGACCGAGC TGCCCGTGAT GGTGGAGCTT 
GCCAGTGACT TCTTGGATAG AAACACTCCA GTCTTTCGAG ATGATGTTTG CTTTTTCATT 
AGTCAATCAG GCGAGACAGC TGACACCCTG ATGGGACTTC GTTACTGTAA GGAGAGAGGA 
GCCTTAACTG TGGGGATCAC AAATACAGTC GGCAGTTCTA TATCAAGGGA GACAGATTGC 
GGGGTTCATA TTAATGCTGG TCCTGAGATT GGCGTGGCCA GTACAAAGGC ATACACCAGC 
CAGTTTGTGT CCCTCGTGAT GTTTGCTCTC ATGATGTGTG ATGACAGGAT CTCCATGCAA 
GAGAGACGCA AAGAGATCAT GCTCGGACTG AAGCGACTGC CGGACTTGAT TAAGGAAGTG 
CTGAGCATGG ATGATGAAAT CCAGAAGCTG GCGACGGAGC TTTACCACCA GAAGTCGGTC 
CTGATAATGG GGCGGGGCTA CCATTATGCT ACATGCCTTG AAGGGGCTCT GAAAATCAAG 
GAGATTACTT ATATGCATTC GGAAGGCATC CTTGCTGGTG AGCTCAAGCA CGGCCCTCTG 
GCCTTGGTGG ACAAGTTGAT GCCTGTCATC ATGATCATCA TGCGAGACCA CACTTATGCC 
AAGTGCCAGA ACGCTCTTCA GCAGGTGGTT GCACGGCAGG GGCGTCCAGT CGTGATCTGT 
GATAAGGAGG ATACTGAGAC CATTAAGAAT ACAAAAAGGA CAATCAAGGT GCCCCACTCA 
GTGGACTGCT TGCAGGGCAT TCTCAGTGTG ATTCCCCTGC AGCTGCTGGC TTTCCACCTG 
GCTGTGCTGA GAGGCTACGA TGTTGATTTT CCACGGAATC TTGCCAAATC TGTAACAGTA 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038556.1
CDS	154..2199
Translation	MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGLDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPNPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNWESQDVSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCGLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFISQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Target ORF information:

RefSeq Version	NM_013528.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamine fructose-6-phosphate transaminase 1 (Gfpt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013528.3

ORF Insert Sequence:

ATGTGCGGTA TATTTGCTTA TTTAAATTAC CATGTTCCTC GAACAAGACG AGAAATCTTG 
GAGACACTAA TCAAAGGCCT TCAGAGACTG GAATACAGAG GATATGATTC TGCTGGTGTG 
GGACTTGACG GAGGCAATGA CAAAGACTGG GAAGCCAACG CCTGCAAAAT CCAGCTCATT 
AAGAAGAAAG GAAAAGTTAA GGCACTGGAT GAAGAAGTTC ACAAACAACA AGATATGGAC 
TTGGATATAG AATTTGATGT GCATCTTGGA ATAGCTCATA CCCGTTGGGC GACACATGGA 
GAACCCAATC CTGTCAATAG TCACCCCCAG CGCTCTGATA AAAATAATGA ATTCATTGTT 
ATTCATAATG GAATCATCAC CAACTACAAA GACTTGAAAA AGTTTCTGGA AAGCAAAGGC 
TATGACTTTG AATCTGAAAC AGACACAGAA ACCATTGCCA AGCTCGTCAA GTACATGTAT 
GACAACTGGG AGAGCCAGGA CGTCAGTTTT ACCACCTTGG TGGAGAGAGT TATCCAACAA 
TTGGAAGGCG CCTTTGCTCT TGTGTTTAAA AGTGTCCATT TTCCCGGGCA AGCAGTTGGC 
ACAAGGCGAG GTAGCCCTCT CTTGATTGGT GTGCGGAGTG AACATAAGCT TTCTACAGAT 
CACATTCCGA TTCTGTACAG AACAGGCAAA GACAAGAAAG GAAGCTGCGG TCTTTCCCGT 
GTGGACAGCA CGACATGCCT GTTCCCTGTT GAGGAAAAGG CAGTTGAATA TTACTTTGCT 
TCTGATGCAA GTGCCGTGAT AGAGCACACC AATCGTGTCA TCTTTCTGGA AGATGATGAT 
GTTGCAGCAG TGGTGGATGG CCGTCTCTCT ATCCACCGAA TTAAACGAAC TGCAGGAGAC 
CATCCTGGCC GAGCTGTGCA AACTCTCCAG ATGGAGCTCC AGCAGATCAT GAAGGGCAAC 
TTTAGTTCAT TTATGCAGAA GGAAATTTTT GAGCAGCCAG AATCTGTTGT GAACACAATG 
AGAGGAAGAG TCAATTTTGA TGACTACACT GTGAATTTGG GAGGTTTGAA AGATCACATT 
AAGGAGATCC AGCGGTGTCG GCGGTTGATT CTTATTGCTT GTGGCACAAG TTACCACGCT 
GGTGTGGCAA CCCGTCAGGT CCTGGAGGAG CTGACCGAGC TGCCCGTGAT GGTGGAGCTT 
GCCAGTGACT TCTTGGATAG AAACACTCCA GTCTTTCGAG ATGATGTTTG CTTTTTCATT 
AGTCAATCAG GCGAGACAGC TGACACCCTG ATGGGACTTC GTTACTGTAA GGAGAGAGGA 
GCCTTAACTG TGGGGATCAC AAATACAGTC GGCAGTTCTA TATCAAGGGA GACAGATTGC 
GGGGTTCATA TTAATGCTGG TCCTGAGATT GGCGTGGCCA GTACAAAGGC ATACACCAGC 
CAGTTTGTGT CCCTCGTGAT GTTTGCTCTC ATGATGTGTG ATGACAGGAT CTCCATGCAA 
GAGAGACGCA AAGAGATCAT GCTCGGACTG AAGCGACTGC CGGACTTGAT TAAGGAAGTG 
CTGAGCATGG ATGATGAAAT CCAGAAGCTG GCGACGGAGC TTTACCACCA GAAGTCGGTC 
CTGATAATGG GGCGGGGCTA CCATTATGCT ACATGCCTTG AAGGGGCTCT GAAAATCAAG 
GAGATTACTT ATATGCATTC GGAAGGCATC CTTGCTGGTG AGCTCAAGCA CGGCCCTCTG 
GCCTTGGTGG ACAAGTTGAT GCCTGTCATC ATGATCATCA TGCGAGACCA CACTTATGCC 
AAGTGCCAGA ACGCTCTTCA GCAGGTGGTT GCACGGCAGG GGCGTCCAGT CGTGATCTGT 
GATAAGGAGG ATACTGAGAC CATTAAGAAT ACAAAAAGGA CAATCAAGGT GCCCCACTCA 
GTGGACTGCT TGCAGGGCAT TCTCAGTGTG ATTCCCCTGC AGCTGCTGGC TTTCCACCTG 
GCTGTGCTGA GAGGCTACGA TGTTGATTTT CCACGGAATC TTGCCAAATC TGTAACAGTA 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

mTORC2 Responds to Glutamine Catabolite Levels to Modulate the Hexosamine Biosynthesis Enzyme GFAT1.
Molecular cell63(5)811-26(2016 Sep)
Moloughney JG,Kim PK,Vega-Cotto NM,Wu CC,Zhang S,Adlam M,Lynch T,Chou PC,Rabinowitz JD,Werlen G,Jacinto E

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

O-GlcNAc signaling entrains the circadian clock by inhibiting BMAL1/CLOCK ubiquitination.
Cell metabolism17(2)303-10(2013 Feb)
Li MD,Ruan HB,Hughes ME,Lee JS,Singh JP,Jones SP,Nitabach MN,Yang X

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Gfpt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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