Gem cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gem
Entrez Gene ID	14579
Full Name	GTP binding protein (gene overexpressed in skeletal muscle)
Synonyms	AV020497
General protein information	Preferred Names GTP binding protein (gene overexpressed in skeletal muscle) Names GTP-binding protein GEM GTP-binding mitogen-induced T-cell protein RAS-like protein KIR
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 A1\|4 5.34 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010276.4	NP_034406.2	GTP-binding protein GEM

Pathways from BioSystems

Homologies

	GEM	XP_001087974.1
Danio rerio (zebrafish)	gem	NP_001039314.1
Rattus norvegicus (Norway rat)	Gem	NP_001100107.1
Xenopus tropicalis (tropical clawed frog)	gem	NP_001090822.1
Homo sapiens (human)	GEM	NP_859053.1
Gallus gallus (chicken)	GEM	NP_998744.1
Pan troglodytes (chimpanzee)	GEM	XP_001142383.1
Canis lupus familiaris (dog)	GEM	XP_854414.2
Bos taurus (cattle)	GEM	NP_001263234.1
Mus musculus (house mouse)	Gem	NP_034406.2

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Gurdziel K, Vogt KR, Walton KD, Schneider GK, Gumucio DL
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)

Geminin loss causes neural tube defects through disrupted progenitor specification and neuronal differentiation.
Patterson ES, Waller LE, Kroll KL
Developmental biology393(1)44-56(2014 Sep)

RGK protein-mediated impairment of slow depolarization- dependent Ca2+ entry into developing myotubes.
Romberg CF, Beqollari D, Meza U, Bannister RA
Channels (Austin, Tex.)8(3)243-8(2014)

Lmx1a is an activator of Rgs4 and Grb10 and is responsible for the correct specification of rostral and medial mdDA neurons.
Hoekstra EJ, von Oerthel L, van der Linden AJ, Schellevis RD, Scheppink G, Holstege FC, Groot-Koerkamp MJ, van der Heide LP, Smidt MP
The European journal of neuroscience37(1)23-32(2013 Jan)

Mice deficient in GEM GTPase show abnormal glucose homeostasis due to defects in beta-cell calcium handling.
Gunton JE, Sisavanh M, Stokes RA, Satin J, Satin LS, Zhang M, Liu SM, Cai W, Cheng K, Cooney GJ, Laybutt DR, So T, Molero JC, Grey ST, Andres DA, Rolph MS, Mackay CR
PloS one7(6)e39462(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rad, Gem and Rem all reduce depolarization-dependent Ca2+ entry in developing myotubes.
Title: RGK protein-mediated impairment of slow depolarization- dependent Ca2+ entry into developing myotubes.

These results suggest that Gem plays an important role in normal beta-cell function by regulation of calcium signalling.
Title: Mice deficient in GEM GTPase show abnormal glucose homeostasis due to defects in beta-cell calcium handling.

Results identify specific nuclear localization signals (NLS) in Kir/Gem that are both required and sufficient for nuclear transport.
Title: Nuclear transport of Kir/Gem requires specific signals and importin alpha5 and is regulated by calmodulin and predicted serine phosphorylations.

docking of the RGK protein to the nucleotide-kinase domain of the Cavbeta subunit is reminiscent of the binding of GMP to guanylate kinase
Title: RGK small GTP-binding proteins interact with the nucleotide kinase domain of Ca2+-channel beta-subunits via an uncommon effector binding domain.

Mechanism of the inhibition of calcium channel activity by Gem.
Title: Direct inhibition of the interaction between alpha-interaction domain and beta-interaction domain of voltage-dependent Ca2+ channels by Gem.

The following Gem gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gem cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05875	NM_010276.4 Latest version!	Mus musculus GTP binding protein (gene overexpressed in skeletal muscle) (Gem), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gem ( NM_010276.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034406.2 Mus musculus GTP binding protein (gene overexpressed in skeletal muscle) (Gem), mRNA.

Price & Availability↑

CloneID	OMu05875
Clone ID Related Accession (Same CDS sequence)	NM_010276.4
Accession Version	NM_010276.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	GTP-binding protein GEM
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158393.1, AK168401.1 and BY574090.1. On Oct 6, 2009 this sequence version replaced NM_010276.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK157655.1, AK168401.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTCTGA ATAATGTCAC CATGCGCCAA GGCACTGTGG GCATGCAGCC ACAGCAGCGC 
TGGAGTATCC CTGCTGATGC CAGGCATCTG ATGGTCCAGA AGGATCCCCA CCCCTGCAAC 
CTCCGAAACC GCCACTCTAC TGCTCCGGAA GAGCACTGCC GGCGGAGCTG GTCGTCCGAC 
TCCACAGACT CGGTCATCTC TTCTGAGTCT GGAAACACCT ACTACCGAGT AGTGCTTATA 
GGGGAGCAAG GAGTGGGCAA GTCCACCCTG GCCAACATCT TTGCAGGTGT GCATGACAGC 
ATGGACAGCG ACTGTGAGGT CTTGGGAGAA GATACATATG AGCGTACCCT GGTCGTTGAC 
GGAGAGAGTG CAACCATTAT CCTCCTGGAC ATGTGGGAAA ATAAGGGGGA GAACGAATGG 
CTCCATGACC ACTGCATGCA GGTCGGGGAT GCCTATCTGA TCGTCTACTC TATCACAGAC 
CGTGCAAGCT TCGAGAAGGC ATCTGAGCTG AGGATTCAGC TCCGCAGGGC CCGGCAGACA 
GAAGACATTC CTATAATTTT GGTTGGCAAC AAAAGCGACT TAGTGCGGTG TCGAGAAGTG 
TCTGTGTCAG AAGGGAGAGC TTGTGCTGTG GTGTTCGACT GCAAATTCAT CGAGACCTCT 
GCGGCTGTGC AGCACAACGT GAAGGAACTG TTTGAGGGCA TTGTGCGACA GGTGCGCCTG 
CGCAGGGACA GCAAGGAGAA GAATGAGAGG AGGCTGGCCT ACCAGAAGAG GCGGGAGAGC 
ATTCCCAGGA AAGCCAGGCG CTTCTGGGGC AAAATTGTGG CCAAAAACAA CAAGAACATG 
GCTTTCAAGC TCAAGTCAAA ATCCTGCCAT GACCTGTCTG TGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034406.2
CDS	130..1017
Translation	MTLNNVTMRQGTVGMQPQQRWSIPADARHLMVQKDPHPCNLRNRHSTAPEEHCRRSWSSDSTDSVISSESGNTYYRVVLIGEQGVGKSTLANIFAGVHDSMDSDCEVLGEDTYERTLVVDGESATIILLDMWENKGENEWLHDHCMQVGDAYLIVYSITDRASFEKASELRIQLRRARQTEDIPIILVGNKSDLVRCREVSVSEGRACAVVFDCKFIETSAAVQHNVKELFEGIVRQVRLRRDSKEKNERRLAYQKRRESIPRKARRFWGKIVAKNNKNMAFKLKSKSCHDLSVL

Target ORF information:

RefSeq Version	NM_010276.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTP binding protein (gene overexpressed in skeletal muscle) (Gem), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010276.4

ORF Insert Sequence:

ATGACTCTGA ATAATGTCAC CATGCGCCAA GGCACTGTGG GCATGCAGCC ACAGCAGCGC 
TGGAGTATCC CTGCTGATGC CAGGCATCTG ATGGTCCAGA AGGATCCCCA CCCCTGCAAC 
CTCCGAAACC GCCACTCTAC TGCTCCGGAA GAGCACTGCC GGCGGAGCTG GTCGTCCGAC 
TCCACAGACT CGGTCATCTC TTCTGAGTCT GGAAACACCT ACTACCGAGT AGTGCTTATA 
GGGGAGCAAG GAGTGGGCAA GTCCACCCTG GCCAACATCT TTGCAGGTGT GCATGACAGC 
ATGGACAGCG ACTGTGAGGT CTTGGGAGAA GATACATATG AGCGTACCCT GGTCGTTGAC 
GGAGAGAGTG CAACCATTAT CCTCCTGGAC ATGTGGGAAA ATAAGGGGGA GAACGAATGG 
CTCCATGACC ACTGCATGCA GGTCGGGGAT GCCTATCTGA TCGTCTACTC TATCACAGAC 
CGTGCAAGCT TCGAGAAGGC ATCTGAGCTG AGGATTCAGC TCCGCAGGGC CCGGCAGACA 
GAAGACATTC CTATAATTTT GGTTGGCAAC AAAAGCGACT TAGTGCGGTG TCGAGAAGTG 
TCTGTGTCAG AAGGGAGAGC TTGTGCTGTG GTGTTCGACT GCAAATTCAT CGAGACCTCT 
GCGGCTGTGC AGCACAACGT GAAGGAACTG TTTGAGGGCA TTGTGCGACA GGTGCGCCTG 
CGCAGGGACA GCAAGGAGAA GAATGAGAGG AGGCTGGCCT ACCAGAAGAG GCGGGAGAGC 
ATTCCCAGGA AAGCCAGGCG CTTCTGGGGC AAAATTGTGG CCAAAAACAA CAAGAACATG 
GCTTTCAAGC TCAAGTCAAA ATCCTGCCAT GACCTGTCTG TGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)
Gurdziel K,Vogt KR,Walton KD,Schneider GK,Gumucio DL

Geminin loss causes neural tube defects through disrupted progenitor specification and neuronal differentiation.
Developmental biology393(1)44-56(2014 Sep)
Patterson ES,Waller LE,Kroll KL

RGK protein-mediated impairment of slow depolarization- dependent Ca2+ entry into developing myotubes.
Channels (Austin, Tex.)8(3)243-8(2014)
Romberg CF,Beqollari D,Meza U,Bannister RA

Lmx1a is an activator of Rgs4 and Grb10 and is responsible for the correct specification of rostral and medial mdDA neurons.
The European journal of neuroscience37(1)23-32(2013 Jan)
Hoekstra EJ,von Oerthel L,van der Linden AJ,Schellevis RD,Scheppink G,Holstege FC,Groot-Koerkamp MJ,van der Heide LP,Smidt MP

Mice deficient in GEM GTPase show abnormal glucose homeostasis due to defects in beta-cell calcium handling.
PloS one7(6)e39462(2012)
Gunton JE,Sisavanh M,Stokes RA,Satin J,Satin LS,Zhang M,Liu SM,Cai W,Cheng K,Cooney GJ,Laybutt DR,So T,Molero JC,Grey ST,Andres DA,Rolph MS,Mackay CR

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Gem cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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