Gapdh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gapdh
Entrez Gene ID	14433
Full Name	glyceraldehyde-3-phosphate dehydrogenase
Synonyms	Gapd
General protein information	Preferred Names glyceraldehyde-3-phosphate dehydrogenase Names glyceraldehyde-3-phosphate dehydrogenase peptidyl-cysteine S-nitrosylase GAPDH
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 F2\|6 59.32 cM
Summary	This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Alternative splicing results in multiple transcript variants. Many pseudogenes similar to this locus are found throughout the mouse genome. [provided by RefSeq, Jan 2014].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017321385.2	XP_017176874.1	glyceraldehyde-3-phosphate dehydrogenase isoform X1
NM_001289726.1	NP_001276655.1	glyceraldehyde-3-phosphate dehydrogenase isoform 1
NM_008084.3	NP_032110.1	glyceraldehyde-3-phosphate dehydrogenase isoform 2
XM_017321385.1	XP_017176874.1	glyceraldehyde-3-phosphate dehydrogenase isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC100688969	XP_003435697.1
Bos taurus (cattle)	GAPDH	NP_001029206.1
Mus musculus (house mouse)	Gapdh	NP_032110.1
Mus musculus (house mouse)	Gm20899	XP_001476757.1
Gallus gallus (chicken)	GAPDH	NP_989636.1
Caenorhabditis elegans (roundworm)	gpd-1	NP_496237.1
Caenorhabditis elegans (roundworm)	gpd-3	NP_508534.3
Saccharomyces cerevisiae (baker's yeast)	TDH2	NP_012542.1
Drosophila melanogaster (fruit fly)	Gapdh1	NP_525108.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009623	XP_318655.2
Caenorhabditis elegans (roundworm)	gpd-4	NP_496192.1
Caenorhabditis elegans (roundworm)	gpd-2	NP_508535.1
Saccharomyces cerevisiae (baker's yeast)	TDH1	NP_012483.3
Kluyveromyces lactis	KLLA0F20988g	XP_456022.1
Schizosaccharomyces pombe (fission yeast)	gpd3	NP_595236.1
Magnaporthe oryzae (rice blast fungus)	MGG_01084	XP_003717853.1
Oryza sativa (rice)	Os08g0126300	NP_001060897.1
	GAPDH	XP_001105471.1
Canis lupus familiaris (dog)	LOC100683724	XP_003434435.1
Rattus norvegicus (Norway rat)	Gapdh	NP_058704.1
Drosophila melanogaster (fruit fly)	Gapdh2	NP_001259584.1
Saccharomyces cerevisiae (baker's yeast)	TDH3	NP_011708.3
Schizosaccharomyces pombe (fission yeast)	tdh1	NP_596154.1
Pan troglodytes (chimpanzee)	GAPDH	XP_508955.1
Canis lupus familiaris (dog)	GAPDH	NP_001003142.1
Homo sapiens (human)	GAPDH	NP_002037.2
Danio rerio (zebrafish)	gapdh	NP_001108586.1
Neurospora crassa	NCU01528	XP_956977.1
Xenopus tropicalis (tropical clawed frog)	gapdh	NP_001004949.1

Gene Ontology
Bibliography

Related articles in PubMed

OTX1 regulates cell cycle progression of neural progenitors in the developing cerebral cortex.
Huang B, Li X, Tu X, Zhao W, Zhu D, Feng Y, Si X, Chen JG
The Journal of biological chemistry(2017 Dec)

GAPDH mediates nitrosylation of nuclear proteins.
Kornberg MD, Sen N, Hara MR, Juluri KR, Nguyen JV, Snowman AM, Law L, Hester LD, Snyder SH
Nature cell biology12(11)1094-100(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that GAPDH is an ancient, evolutionarily conserved receptor that plays a key role in the Plg-dependent regulation of macrophage recruitment in the inflammatory response to microbial aggression, thus pitting prokaryotic GAPDH against mammalian GAPDH, with both involved in a conserved role of Plg activation on the surface of their respective cells, to conflicting ends.
Title: Moonlighting glycolytic protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH): an evolutionarily conserved plasminogen receptor on mammalian cells.

These findings show that post-translational modification of GAPDH lysine residues regulates hepatic and systemic metabolism, revealing an unappreciated role for hepatic GAPDH in substrate selection and utilization.
Title: Lysine post-translational modification of glyceraldehyde-3-phosphate dehydrogenase regulates hepatic and systemic metabolism.

GAPDH-mediated expression regulation of sodium channel genes was associated with epilepsy and the anticonvulsant action of the ketogenic diet.
Title: GAPDH-mediated posttranscriptional regulations of sodium channel Scn1a and Scn3a genes under seizure and ketogenic diet conditions.

Exosomal GAPDH from Proximal Tubule Cells Regulate ENaC Activity
Title: Exosomal GAPDH from Proximal Tubule Cells Regulate ENaC Activity.

Data indicate that lactoferrin uptake across duodenum by glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Title: Secreted multifunctional Glyceraldehyde-3-phosphate dehydrogenase sequesters lactoferrin and iron into cells via a non-canonical pathway.

The following Gapdh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gapdh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23266	NM_001289726.1 Latest version!	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu19989	NM_008084.3 Latest version!	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu67802	XM_017321385.2 Latest version!	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu67802	XM_017321385.1	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gapdh ( NM_001289726.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276655.1 Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23266
Clone ID Related Accession (Same CDS sequence)	NM_001289726.1
Accession Version	NM_001289726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-14
Organism	Mus musculus(house mouse)
Product	glyceraldehyde-3-phosphate dehydrogenase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC166162.6, AK140794.1 and GU214026.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK144690.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 17121833, 18809573, 20972425 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCCCT TACCCCGGGG TCCCAGCTTA GGTTCATCAG GTAAACTCAG GAGAGTGTTT 
CCTCGTCCCG TAGACAAAAT GGTGAAGGTC GGTGTGAACG GATTTGGCCG TATTGGGCGC 
CTGGTCACCA GGGCTGCCAT TTGCAGTGGC AAAGTGGAGA TTGTTGCCAT CAACGACCCC 
TTCATTGACC TCAACTACAT GGTCTACATG TTCCAGTATG ACTCCACTCA CGGCAAATTC 
AACGGCACAG TCAAGGCCGA GAATGGGAAG CTTGTCATCA ACGGGAAGCC CATCACCATC 
TTCCAGGAGC GAGACCCCAC TAACATCAAA TGGGGTGAGG CCGGTGCTGA GTATGTCGTG 
GAGTCTACTG GTGTCTTCAC CACCATGGAG AAGGCCGGGG CCCACTTGAA GGGTGGAGCC 
AAAAGGGTCA TCATCTCCGC CCCTTCTGCC GATGCCCCCA TGTTTGTGAT GGGTGTGAAC 
CACGAGAAAT ATGACAACTC ACTCAAGATT GTCAGCAATG CATCCTGCAC CACCAACTGC 
TTAGCCCCCC TGGCCAAGGT CATCCATGAC AACTTTGGCA TTGTGGAAGG GCTCATGACC 
ACAGTCCATG CCATCACTGC CACCCAGAAG ACTGTGGATG GCCCCTCTGG AAAGCTGTGG 
CGTGATGGCC GTGGGGCTGC CCAGAACATC ATCCCTGCAT CCACTGGTGC TGCCAAGGCT 
GTGGGCAAGG TCATCCCAGA GCTGAACGGG AAGCTCACTG GCATGGCCTT CCGTGTTCCT 
ACCCCCAATG TGTCCGTCGT GGATCTGACG TGCCGCCTGG AGAAACCTGC CAAGTATGAT 
GACATCAAGA AGGTGGTGAA GCAGGCATCT GAGGGCCCAC TGAAGGGCAT CTTGGGCTAC 
ACTGAGGACC AGGTTGTCTC CTGCGACTTC AACAGCAACT CCCACTCTTC CACCTTCGAT 
GCCGGGGCTG GCATTGCTCT CAATGACAAC TTTGTCAAGC TCATTTCCTG GTATGACAAT 
GAATACGGCT ACAGCAACAG GGTGGTGGAC CTCATGGCCT ACATGGCCTC CAAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276655.1
CDS	15..1094
Translation	MLPLPRGPSLGSSGKLRRVFPRPVDKMVKVGVNGFGRIGRLVTRAAICSGKVEIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE

Target ORF information:

RefSeq Version	NM_001289726.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289726.1

ORF Insert Sequence:

ATGCTGCCCT TACCCCGGGG TCCCAGCTTA GGTTCATCAG GTAAACTCAG GAGAGTGTTT 
CCTCGTCCCG TAGACAAAAT GGTGAAGGTC GGTGTGAACG GATTTGGCCG TATTGGGCGC 
CTGGTCACCA GGGCTGCCAT TTGCAGTGGC AAAGTGGAGA TTGTTGCCAT CAACGACCCC 
TTCATTGACC TCAACTACAT GGTCTACATG TTCCAGTATG ACTCCACTCA CGGCAAATTC 
AACGGCACAG TCAAGGCCGA GAATGGGAAG CTTGTCATCA ACGGGAAGCC CATCACCATC 
TTCCAGGAGC GAGACCCCAC TAACATCAAA TGGGGTGAGG CCGGTGCTGA GTATGTCGTG 
GAGTCTACTG GTGTCTTCAC CACCATGGAG AAGGCCGGGG CCCACTTGAA GGGTGGAGCC 
AAAAGGGTCA TCATCTCCGC CCCTTCTGCC GATGCCCCCA TGTTTGTGAT GGGTGTGAAC 
CACGAGAAAT ATGACAACTC ACTCAAGATT GTCAGCAATG CATCCTGCAC CACCAACTGC 
TTAGCCCCCC TGGCCAAGGT CATCCATGAC AACTTTGGCA TTGTGGAAGG GCTCATGACC 
ACAGTCCATG CCATCACTGC CACCCAGAAG ACTGTGGATG GCCCCTCTGG AAAGCTGTGG 
CGTGATGGCC GTGGGGCTGC CCAGAACATC ATCCCTGCAT CCACTGGTGC TGCCAAGGCT 
GTGGGCAAGG TCATCCCAGA GCTGAACGGG AAGCTCACTG GCATGGCCTT CCGTGTTCCT 
ACCCCCAATG TGTCCGTCGT GGATCTGACG TGCCGCCTGG AGAAACCTGC CAAGTATGAT 
GACATCAAGA AGGTGGTGAA GCAGGCATCT GAGGGCCCAC TGAAGGGCAT CTTGGGCTAC 
ACTGAGGACC AGGTTGTCTC CTGCGACTTC AACAGCAACT CCCACTCTTC CACCTTCGAT 
GCCGGGGCTG GCATTGCTCT CAATGACAAC TTTGTCAAGC TCATTTCCTG GTATGACAAT 
GAATACGGCT ACAGCAACAG GGTGGTGGAC CTCATGGCCT ACATGGCCTC CAAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gapdh ( XM_017321385.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176874.1 Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67802
Clone ID Related Accession (Same CDS sequence)	XM_017321385.2 , XM_017321385.1
Accession Version	XM_017321385.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glyceraldehyde-3-phosphate dehydrogenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGG TCGGTGTGAA CGGATTTGGC CGTATTGGGC GCCTGGTCAC CAGGGCTGCC 
ATTTGCAGTG GCAAAGTGGA GATTGTTGCC ATCAACGACC CCTTCATTGA CCTCAACTAC 
ATGGTCTACA TGTTCCAGTA TGACTCCACT CACGGCAAAT TCAACGGCAC AGTCAAGGCC 
GAGAATGGGA AGCTTGTCAT CAACGGGAAG CCCATCACCA TCTTCCAGGA GCGAGACCCC 
ACTAACATCA AATGGGGTGA GGCCGGTGCT GAGTATGTCG TGGAGTCTAC TGGTGTCTTC 
ACCACCATGG AGAAGGCCGG GGCCCACTTG AAGGGTGGAG CCAAAAGGGT CATCATCTCC 
GCCCCTTCTG CCGATGCCCC CATGTTTGTG ATGGGTGTGA ACCACGAGAA ATATGACAAC 
TCACTCAAGA TTGTCAGCAA TGCATCCTGC ACCACCAACT GCTTAGCCCC CCTGGCCAAG 
GTCATCCATG ACAACTTTGG CATTGTGGAA GGGCTCATGA CCACAGTCCA TGCCATCACT 
GCCACCCAGA AGACTGTGGA TGGCCCCTCT GGAAAGCTGT GGCGTGATGG CCGTGGGGCT 
GCCCAGAACA TCATCCCTGC ATCCACTGGT GCTGCCAAGG CTGTGGGCAA GGTCATCCCA 
GAGCTGAACG GGAAGCTCAC TGGCATGGCC TTCCGTGTTC CTACCCCCAA TGTGTCCGTC 
GTGGATCTGA CGTGCCGCCT GGAGAAACCT GTATGTATGG GGAGAGCTGG GCTTGTCTCT 
GGTGTGACAG TGACTTGGGA CAAGGATAGT CATTTTGGGG TTTGTTCTTA TCAGGCCAAG 
TATGATGACA TCAAGAAGGT GGTGAAGCAG GCATCTGAGG GCCCACTGAA GGGCATCTTG 
GGCTACACTG AGGACCAGGT TGTCTCCTGC GACTTCAACA GCAACTCCCA CTCTTCCACC 
TTCGATGCCG GGGCTGGCAT TGCTCTCAAT GACAACTTTG TCAAGCTCAT TTCCTGGTAT 
GTGGGGATGG GAAACCTGAC TTTTAAGAGC AACTGGGGGT TTGGTGCCCT CTGGTGGCTA 
GCTCAGAAAA GAAACCCAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176874.1
CDS	442..1545
Translation	MVKVGVNGFGRIGRLVTRAAICSGKVEIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPVCMGRAGLVSGVTVTWDKDSHFGVCSYQAKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYVGMGNLTFKSNWGFGALWWLAQKRNPN

Target ORF information:

RefSeq Version	XM_017321385.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321385.1

ORF Insert Sequence:

ATGGTGAAGG TCGGTGTGAA CGGATTTGGC CGTATTGGGC GCCTGGTCAC CAGGGCTGCC 
ATTTGCAGTG GCAAAGTGGA GATTGTTGCC ATCAACGACC CCTTCATTGA CCTCAACTAC 
ATGGTCTACA TGTTCCAGTA TGACTCCACT CACGGCAAAT TCAACGGCAC AGTCAAGGCC 
GAGAATGGGA AGCTTGTCAT CAACGGGAAG CCCATCACCA TCTTCCAGGA GCGAGACCCC 
ACTAACATCA AATGGGGTGA GGCCGGTGCT GAGTATGTCG TGGAGTCTAC TGGTGTCTTC 
ACCACCATGG AGAAGGCCGG GGCCCACTTG AAGGGTGGAG CCAAAAGGGT CATCATCTCC 
GCCCCTTCTG CCGATGCCCC CATGTTTGTG ATGGGTGTGA ACCACGAGAA ATATGACAAC 
TCACTCAAGA TTGTCAGCAA TGCATCCTGC ACCACCAACT GCTTAGCCCC CCTGGCCAAG 
GTCATCCATG ACAACTTTGG CATTGTGGAA GGGCTCATGA CCACAGTCCA TGCCATCACT 
GCCACCCAGA AGACTGTGGA TGGCCCCTCT GGAAAGCTGT GGCGTGATGG CCGTGGGGCT 
GCCCAGAACA TCATCCCTGC ATCCACTGGT GCTGCCAAGG CTGTGGGCAA GGTCATCCCA 
GAGCTGAACG GGAAGCTCAC TGGCATGGCC TTCCGTGTTC CTACCCCCAA TGTGTCCGTC 
GTGGATCTGA CGTGCCGCCT GGAGAAACCT GTATGTATGG GGAGAGCTGG GCTTGTCTCT 
GGTGTGACAG TGACTTGGGA CAAGGATAGT CATTTTGGGG TTTGTTCTTA TCAGGCCAAG 
TATGATGACA TCAAGAAGGT GGTGAAGCAG GCATCTGAGG GCCCACTGAA GGGCATCTTG 
GGCTACACTG AGGACCAGGT TGTCTCCTGC GACTTCAACA GCAACTCCCA CTCTTCCACC 
TTCGATGCCG GGGCTGGCAT TGCTCTCAAT GACAACTTTG TCAAGCTCAT TTCCTGGTAT 
GTGGGGATGG GAAACCTGAC TTTTAAGAGC AACTGGGGGT TTGGTGCCCT CTGGTGGCTA 
GCTCAGAAAA GAAACCCAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gapdh ( NM_008084.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032110.1 Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19989
Clone ID Related Accession (Same CDS sequence)	NM_008084.3
Accession Version	NM_008084.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	glyceraldehyde-3-phosphate dehydrogenase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ155387.1, AK140794.1 and GU214026.1. On Jan 27, 2014 this sequence version replaced NM_008084.2. Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GU214026.1, BC096440.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 17121833, 18809573, 20972425 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGG TCGGTGTGAA CGGATTTGGC CGTATTGGGC GCCTGGTCAC CAGGGCTGCC 
ATTTGCAGTG GCAAAGTGGA GATTGTTGCC ATCAACGACC CCTTCATTGA CCTCAACTAC 
ATGGTCTACA TGTTCCAGTA TGACTCCACT CACGGCAAAT TCAACGGCAC AGTCAAGGCC 
GAGAATGGGA AGCTTGTCAT CAACGGGAAG CCCATCACCA TCTTCCAGGA GCGAGACCCC 
ACTAACATCA AATGGGGTGA GGCCGGTGCT GAGTATGTCG TGGAGTCTAC TGGTGTCTTC 
ACCACCATGG AGAAGGCCGG GGCCCACTTG AAGGGTGGAG CCAAAAGGGT CATCATCTCC 
GCCCCTTCTG CCGATGCCCC CATGTTTGTG ATGGGTGTGA ACCACGAGAA ATATGACAAC 
TCACTCAAGA TTGTCAGCAA TGCATCCTGC ACCACCAACT GCTTAGCCCC CCTGGCCAAG 
GTCATCCATG ACAACTTTGG CATTGTGGAA GGGCTCATGA CCACAGTCCA TGCCATCACT 
GCCACCCAGA AGACTGTGGA TGGCCCCTCT GGAAAGCTGT GGCGTGATGG CCGTGGGGCT 
GCCCAGAACA TCATCCCTGC ATCCACTGGT GCTGCCAAGG CTGTGGGCAA GGTCATCCCA 
GAGCTGAACG GGAAGCTCAC TGGCATGGCC TTCCGTGTTC CTACCCCCAA TGTGTCCGTC 
GTGGATCTGA CGTGCCGCCT GGAGAAACCT GCCAAGTATG ATGACATCAA GAAGGTGGTG 
AAGCAGGCAT CTGAGGGCCC ACTGAAGGGC ATCTTGGGCT ACACTGAGGA CCAGGTTGTC 
TCCTGCGACT TCAACAGCAA CTCCCACTCT TCCACCTTCG ATGCCGGGGC TGGCATTGCT 
CTCAATGACA ACTTTGTCAA GCTCATTTCC TGGTATGACA ATGAATACGG CTACAGCAAC 
AGGGTGGTGG ACCTCATGGC CTACATGGCC TCCAAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032110.1
CDS	241..1242
Translation	MVKVGVNGFGRIGRLVTRAAICSGKVEIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE

Target ORF information:

RefSeq Version	NM_008084.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008084.3

ORF Insert Sequence:

ATGGTGAAGG TCGGTGTGAA CGGATTTGGC CGTATTGGGC GCCTGGTCAC CAGGGCTGCC 
ATTTGCAGTG GCAAAGTGGA GATTGTTGCC ATCAACGACC CCTTCATTGA CCTCAACTAC 
ATGGTCTACA TGTTCCAGTA TGACTCCACT CACGGCAAAT TCAACGGCAC AGTCAAGGCC 
GAGAATGGGA AGCTTGTCAT CAACGGGAAG CCCATCACCA TCTTCCAGGA GCGAGACCCC 
ACTAACATCA AATGGGGTGA GGCCGGTGCT GAGTATGTCG TGGAGTCTAC TGGTGTCTTC 
ACCACCATGG AGAAGGCCGG GGCCCACTTG AAGGGTGGAG CCAAAAGGGT CATCATCTCC 
GCCCCTTCTG CCGATGCCCC CATGTTTGTG ATGGGTGTGA ACCACGAGAA ATATGACAAC 
TCACTCAAGA TTGTCAGCAA TGCATCCTGC ACCACCAACT GCTTAGCCCC CCTGGCCAAG 
GTCATCCATG ACAACTTTGG CATTGTGGAA GGGCTCATGA CCACAGTCCA TGCCATCACT 
GCCACCCAGA AGACTGTGGA TGGCCCCTCT GGAAAGCTGT GGCGTGATGG CCGTGGGGCT 
GCCCAGAACA TCATCCCTGC ATCCACTGGT GCTGCCAAGG CTGTGGGCAA GGTCATCCCA 
GAGCTGAACG GGAAGCTCAC TGGCATGGCC TTCCGTGTTC CTACCCCCAA TGTGTCCGTC 
GTGGATCTGA CGTGCCGCCT GGAGAAACCT GCCAAGTATG ATGACATCAA GAAGGTGGTG 
AAGCAGGCAT CTGAGGGCCC ACTGAAGGGC ATCTTGGGCT ACACTGAGGA CCAGGTTGTC 
TCCTGCGACT TCAACAGCAA CTCCCACTCT TCCACCTTCG ATGCCGGGGC TGGCATTGCT 
CTCAATGACA ACTTTGTCAA GCTCATTTCC TGGTATGACA ATGAATACGG CTACAGCAAC 
AGGGTGGTGG ACCTCATGGC CTACATGGCC TCCAAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gapdh ( XM_017321385.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176874.1 Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant X1, mRNA.

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CloneID	OMu67802
Clone ID Related Accession (Same CDS sequence)	XM_017321385.2 , XM_017321385.1
Accession Version	XM_017321385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glyceraldehyde-3-phosphate dehydrogenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGG TCGGTGTGAA CGGATTTGGC CGTATTGGGC GCCTGGTCAC CAGGGCTGCC 
ATTTGCAGTG GCAAAGTGGA GATTGTTGCC ATCAACGACC CCTTCATTGA CCTCAACTAC 
ATGGTCTACA TGTTCCAGTA TGACTCCACT CACGGCAAAT TCAACGGCAC AGTCAAGGCC 
GAGAATGGGA AGCTTGTCAT CAACGGGAAG CCCATCACCA TCTTCCAGGA GCGAGACCCC 
ACTAACATCA AATGGGGTGA GGCCGGTGCT GAGTATGTCG TGGAGTCTAC TGGTGTCTTC 
ACCACCATGG AGAAGGCCGG GGCCCACTTG AAGGGTGGAG CCAAAAGGGT CATCATCTCC 
GCCCCTTCTG CCGATGCCCC CATGTTTGTG ATGGGTGTGA ACCACGAGAA ATATGACAAC 
TCACTCAAGA TTGTCAGCAA TGCATCCTGC ACCACCAACT GCTTAGCCCC CCTGGCCAAG 
GTCATCCATG ACAACTTTGG CATTGTGGAA GGGCTCATGA CCACAGTCCA TGCCATCACT 
GCCACCCAGA AGACTGTGGA TGGCCCCTCT GGAAAGCTGT GGCGTGATGG CCGTGGGGCT 
GCCCAGAACA TCATCCCTGC ATCCACTGGT GCTGCCAAGG CTGTGGGCAA GGTCATCCCA 
GAGCTGAACG GGAAGCTCAC TGGCATGGCC TTCCGTGTTC CTACCCCCAA TGTGTCCGTC 
GTGGATCTGA CGTGCCGCCT GGAGAAACCT GTATGTATGG GGAGAGCTGG GCTTGTCTCT 
GGTGTGACAG TGACTTGGGA CAAGGATAGT CATTTTGGGG TTTGTTCTTA TCAGGCCAAG 
TATGATGACA TCAAGAAGGT GGTGAAGCAG GCATCTGAGG GCCCACTGAA GGGCATCTTG 
GGCTACACTG AGGACCAGGT TGTCTCCTGC GACTTCAACA GCAACTCCCA CTCTTCCACC 
TTCGATGCCG GGGCTGGCAT TGCTCTCAAT GACAACTTTG TCAAGCTCAT TTCCTGGTAT 
GTGGGGATGG GAAACCTGAC TTTTAAGAGC AACTGGGGGT TTGGTGCCCT CTGGTGGCTA 
GCTCAGAAAA GAAACCCAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176874.1
CDS	438..1541
Translation	MVKVGVNGFGRIGRLVTRAAICSGKVEIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPVCMGRAGLVSGVTVTWDKDSHFGVCSYQAKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYVGMGNLTFKSNWGFGALWWLAQKRNPN

Target ORF information:

RefSeq Version	XM_017321385.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321385.2

ORF Insert Sequence:

ATGGTGAAGG TCGGTGTGAA CGGATTTGGC CGTATTGGGC GCCTGGTCAC CAGGGCTGCC 
ATTTGCAGTG GCAAAGTGGA GATTGTTGCC ATCAACGACC CCTTCATTGA CCTCAACTAC 
ATGGTCTACA TGTTCCAGTA TGACTCCACT CACGGCAAAT TCAACGGCAC AGTCAAGGCC 
GAGAATGGGA AGCTTGTCAT CAACGGGAAG CCCATCACCA TCTTCCAGGA GCGAGACCCC 
ACTAACATCA AATGGGGTGA GGCCGGTGCT GAGTATGTCG TGGAGTCTAC TGGTGTCTTC 
ACCACCATGG AGAAGGCCGG GGCCCACTTG AAGGGTGGAG CCAAAAGGGT CATCATCTCC 
GCCCCTTCTG CCGATGCCCC CATGTTTGTG ATGGGTGTGA ACCACGAGAA ATATGACAAC 
TCACTCAAGA TTGTCAGCAA TGCATCCTGC ACCACCAACT GCTTAGCCCC CCTGGCCAAG 
GTCATCCATG ACAACTTTGG CATTGTGGAA GGGCTCATGA CCACAGTCCA TGCCATCACT 
GCCACCCAGA AGACTGTGGA TGGCCCCTCT GGAAAGCTGT GGCGTGATGG CCGTGGGGCT 
GCCCAGAACA TCATCCCTGC ATCCACTGGT GCTGCCAAGG CTGTGGGCAA GGTCATCCCA 
GAGCTGAACG GGAAGCTCAC TGGCATGGCC TTCCGTGTTC CTACCCCCAA TGTGTCCGTC 
GTGGATCTGA CGTGCCGCCT GGAGAAACCT GTATGTATGG GGAGAGCTGG GCTTGTCTCT 
GGTGTGACAG TGACTTGGGA CAAGGATAGT CATTTTGGGG TTTGTTCTTA TCAGGCCAAG 
TATGATGACA TCAAGAAGGT GGTGAAGCAG GCATCTGAGG GCCCACTGAA GGGCATCTTG 
GGCTACACTG AGGACCAGGT TGTCTCCTGC GACTTCAACA GCAACTCCCA CTCTTCCACC 
TTCGATGCCG GGGCTGGCAT TGCTCTCAAT GACAACTTTG TCAAGCTCAT TTCCTGGTAT 
GTGGGGATGG GAAACCTGAC TTTTAAGAGC AACTGGGGGT TTGGTGCCCT CTGGTGGCTA 
GCTCAGAAAA GAAACCCAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

OTX1 regulates cell cycle progression of neural progenitors in the developing cerebral cortex.
The Journal of biological chemistry(2017 Dec)
Huang B,Li X,Tu X,Zhao W,Zhu D,Feng Y,Si X,Chen JG

GAPDH mediates nitrosylation of nuclear proteins.
Nature cell biology12(11)1094-100(2010 Nov)
Kornberg MD,Sen N,Hara MR,Juluri KR,Nguyen JV,Snowman AM,Law L,Hester LD,Snyder SH

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Gapdh cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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