Gamt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gamt
Entrez Gene ID	14431
Full Name	guanidinoacetate methyltransferase
Synonyms	AA571402,Spintz1
General protein information	Preferred Names guanidinoacetate methyltransferase Names guanidinoacetate N-methyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 C1\|10 39.72 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010255.3	NP_034385.1	guanidinoacetate N-methyltransferase
NM_001347119.1	NP_001334048.1	guanidinoacetate N-methyltransferase isoform 2
NM_010255.4	NP_034385.1	guanidinoacetate N-methyltransferase isoform 1
XM_006513222.3	XP_006513285.1	guanidinoacetate N-methyltransferase isoform X2
XM_006513222.4	XP_006513285.1	guanidinoacetate N-methyltransferase isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GAMT	NP_000147.1
	GAMT	XP_001117362.2
Bos taurus (cattle)	GAMT	NP_001033633.1
Danio rerio (zebrafish)	gamt	NP_001099065.1
Pan troglodytes (chimpanzee)	GAMT	XP_001172605.2
Xenopus tropicalis (tropical clawed frog)	gamt	NP_988896.1
Rattus norvegicus (Norway rat)	Gamt	NP_036925.2
Mus musculus (house mouse)	Gamt	NP_034385.1

Gene Ontology
Bibliography

Related articles in PubMed

Cell-Type-Specific Spatiotemporal Expression of Creatine Biosynthetic Enzyme S-adenosylmethionine:guanidinoacetate N-methyltransferase in Developing Mouse Brain.
Tachikawa M, Watanabe M, Fukaya M, Sakai K, Terasaki T, Hosoya KI
Neurochemical research(2017 Dec)

Creatine Enhances Mitochondrial-Mediated Oligodendrocyte Survival After Demyelinating Injury.
Chamberlain KA, Chapey KS, Nanescu SE, Huang JK
The Journal of neuroscience : the official journal of the Society for Neuroscience37(6)1479-1492(2017 Feb)

Unchanged mitochondrial organization and compartmentation of high-energy phosphates in creatine-deficient GAMT-/- mouse hearts.
Branovets J, Sepp M, Kotlyarova S, Jepihhina N, Sokolova N, Aksentijevic D, Lygate CA, Neubauer S, Vendelin M, Birkedal R
American journal of physiology. Heart and circulatory physiology305(4)H506-20(2013 Aug)

A role for thioredoxin-interacting protein (Txnip) in cellular creatine homeostasis.
Zervou S, Ray T, Sahgal N, Sebag-Montefiore L, Cross R, Medway DJ, Ostrowski PJ, Neubauer S, Lygate CA
American journal of physiology. Endocrinology and metabolism305(2)E263-70(2013 Jul)

Living without creatine: unchanged exercise capacity and response to chronic myocardial infarction in creatine-deficient mice.
Lygate CA, Aksentijevic D, Dawson D, ten Hove M, Phillips D, de Bono JP, Medway DJ, Sebag-Montefiore L, Hunyor I, Channon KM, Clarke K, Zervou S, Watkins H, Balaban RS, Neubauer S
Circulation research112(6)945-55(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inactivation of the creatine kinase system by GAMT deficiency does not alter mitochondrial organization and intracellular compartmentation in relaxed cardiomyocytes.
Title: Unchanged mitochondrial organization and compartmentation of high-energy phosphates in creatine-deficient GAMT-/- mouse hearts.

In the fetal brain, mRNA expression of GAMT was relatively low until 34 days gestation (term is 38-39 days). In the fetal kidney and liver, GAMT mRNA and protein expression were also relatively low until 34-37 days gestation.
Title: Developmental changes in the expression of creatine synthesizing enzymes and creatine transporter in a precocial rodent, the spiny mouse.

GAMT and AGAT mRNA are up-regulated 5.4- and 1.9-fold respectively in adult mdx muscle compared to C57
Title: Upregulation of the creatine synthetic pathway in skeletal muscles of mature mdx mice.

Our results can provide the basis for additional experiments to optimize Cr supplementation in GAMT deficiency, as increases in brain Cr are slow in patients after Cr supplementation.
Title: Creatine uptake in brain and skeletal muscle of mice lacking guanidinoacetate methyltransferase assessed by magnetic resonance spectroscopy.

GAMT(Cr)-/- mice showed differences in maximal rates of force rise and relaxation times during the isometric protocol as well as in force during the dynamic protocol.
Title: Lower force and impaired performance during high-intensity electrical stimulation in skeletal muscle of GAMT-deficient knockout mice.

The following Gamt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gamt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05841	NM_010255.4 Latest version!	Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
OMu44203	XM_006513222.4 Latest version!	Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu44202	NM_001347119.1 Latest version!	Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OMu05841	NM_010255.3	Mus musculus guanidinoacetate methyltransferase (Gamt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
OMu44203	XM_006513222.3	Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gamt ( NM_010255.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034385.1 Mus musculus guanidinoacetate methyltransferase (Gamt), mRNA.

Price & Availability↑

CloneID	OMu05841
Clone ID Related Accession (Same CDS sequence)	NM_010255.3 , NM_010255.4
Accession Version	NM_010255.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	guanidinoacetate N-methyltransferase
Comment

ATGAGCTCTT CTGCAGCTAG CCCGCTCTTC GCGCCCGGCG AGGACTGCGG CCCCGCGTGG 
CGCGCGGCCC CCGCGGCCTA TGACGCGTCT GACACGCACC TGCAAATCCT GGGCAAGCCA 
GTGATGGAGC GTTGGGAGAC CCCCTATATG CATGCGCTAG CGGCGGCTGC TGCCTCCAGA 
GGGGGCCGGG TCTTGGAAGT GGGCTTCGGT ATGGCCATTG CAGCCTCCAG GGTGCAACAG 
GCCCCCATAG AGGAACACTG GATTATTGAG TGCAATGATG GGGTCTTCCA GCGTCTACAA 
GACTGGGCCC TGCGGCAGCC ACATAAGGTT GTTCCCTTGA AAGGCCTGTG GGAGGAGGTG 
GCACCTACCC TGCCTGACGG TCACTTTGAT GGGATTCTAT ATGACACGTA CCCGCTGTCT 
GAAGAGGCCT GGCACACTCA CCAGTTCAAC TTTATTAAGA ATCATGCCTT CCGCTTGCTG 
AAGACCGGGG GCGTCCTCAC CTACTGCAAC CTCACGTCCT GGGGGGAGCT CATGAAGTCC 
AAATACACAG ACATCACCAC CATGTTTGAG GAGACGCAGG TGCCTGCACT GCAGGAAGCT 
GGCTTCCTGA AAGAAAACAT CTGCACAGAG GTGATGGCAC TGGTGCCCCC AGCCGACTGC 
CGCTACTATG CCTTCCCTCA GATGATCACA CCCCTGGTCA CCAAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034385.1
Translation	MSSSAASPLFAPGEDCGPAWRAAPAAYDASDTHLQILGKPVMERWETPYMHALAAAAASRGGRVLEVGFGMAIAASRVQQAPIEEHWIIECNDGVFQRLQDWALRQPHKVVPLKGLWEEVAPTLPDGHFDGILYDTYPLSEEAWHTHQFNFIKNHAFRLLKTGGVLTYCNLTSWGELMKSKYTDITTMFEETQVPALQEAGFLKENICTEVMALVPPADCRYYAFPQMITPLVTKH

Target ORF information:

RefSeq Version	NM_010255.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus guanidinoacetate methyltransferase (Gamt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010255.3

ORF Insert Sequence:

ATGAGCTCTT CTGCAGCTAG CCCGCTCTTC GCGCCCGGCG AGGACTGCGG CCCCGCGTGG 
CGCGCGGCCC CCGCGGCCTA TGACGCGTCT GACACGCACC TGCAAATCCT GGGCAAGCCA 
GTGATGGAGC GTTGGGAGAC CCCCTATATG CATGCGCTAG CGGCGGCTGC TGCCTCCAGA 
GGGGGCCGGG TCTTGGAAGT GGGCTTCGGT ATGGCCATTG CAGCCTCCAG GGTGCAACAG 
GCCCCCATAG AGGAACACTG GATTATTGAG TGCAATGATG GGGTCTTCCA GCGTCTACAA 
GACTGGGCCC TGCGGCAGCC ACATAAGGTT GTTCCCTTGA AAGGCCTGTG GGAGGAGGTG 
GCACCTACCC TGCCTGACGG TCACTTTGAT GGGATTCTAT ATGACACGTA CCCGCTGTCT 
GAAGAGGCCT GGCACACTCA CCAGTTCAAC TTTATTAAGA ATCATGCCTT CCGCTTGCTG 
AAGACCGGGG GCGTCCTCAC CTACTGCAAC CTCACGTCCT GGGGGGAGCT CATGAAGTCC 
AAATACACAG ACATCACCAC CATGTTTGAG GAGACGCAGG TGCCTGCACT GCAGGAAGCT 
GGCTTCCTGA AAGAAAACAT CTGCACAGAG GTGATGGCAC TGGTGCCCCC AGCCGACTGC 
CGCTACTATG CCTTCCCTCA GATGATCACA CCCCTGGTCA CCAAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gamt ( NM_010255.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034385.1 Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05841
Clone ID Related Accession (Same CDS sequence)	NM_010255.3 , NM_010255.4
Accession Version	NM_010255.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-18
Organism	Mus musculus(house mouse)
Product	guanidinoacetate N-methyltransferase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA222404.1, AC140229.3, AK140688.1, AK158087.1 and BE992264.1. On Nov 16, 2016 this sequence version replaced NM_010255.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049233.1, BI331208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849386 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCTT CTGCAGCTAG CCCGCTCTTC GCGCCCGGCG AGGACTGCGG CCCCGCGTGG 
CGCGCGGCCC CCGCGGCCTA TGACGCGTCT GACACGCACC TGCAAATCCT GGGCAAGCCA 
GTGATGGAGC GTTGGGAGAC CCCCTATATG CATGCGCTAG CGGCGGCTGC TGCCTCCAGA 
GGGGGCCGGG TCTTGGAAGT GGGCTTCGGT ATGGCCATTG CAGCCTCCAG GGTGCAACAG 
GCCCCCATAG AGGAACACTG GATTATTGAG TGCAATGATG GGGTCTTCCA GCGTCTACAA 
GACTGGGCCC TGCGGCAGCC ACATAAGGTT GTTCCCTTGA AAGGCCTGTG GGAGGAGGTG 
GCACCTACCC TGCCTGACGG TCACTTTGAT GGGATTCTAT ATGACACGTA CCCGCTGTCT 
GAAGAGGCCT GGCACACTCA CCAGTTCAAC TTTATTAAGA ATCATGCCTT CCGCTTGCTG 
AAGACCGGGG GCGTCCTCAC CTACTGCAAC CTCACGTCCT GGGGGGAGCT CATGAAGTCC 
AAATACACAG ACATCACCAC CATGTTTGAG GAGACGCAGG TGCCTGCACT GCAGGAAGCT 
GGCTTCCTGA AAGAAAACAT CTGCACAGAG GTGATGGCAC TGGTGCCCCC AGCCGACTGC 
CGCTACTATG CCTTCCCTCA GATGATCACA CCCCTGGTCA CCAAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034385.1
CDS	112..822
Translation	MSSSAASPLFAPGEDCGPAWRAAPAAYDASDTHLQILGKPVMERWETPYMHALAAAAASRGGRVLEVGFGMAIAASRVQQAPIEEHWIIECNDGVFQRLQDWALRQPHKVVPLKGLWEEVAPTLPDGHFDGILYDTYPLSEEAWHTHQFNFIKNHAFRLLKTGGVLTYCNLTSWGELMKSKYTDITTMFEETQVPALQEAGFLKENICTEVMALVPPADCRYYAFPQMITPLVTKH

Target ORF information:

RefSeq Version	NM_010255.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010255.4

ORF Insert Sequence:

ATGAGCTCTT CTGCAGCTAG CCCGCTCTTC GCGCCCGGCG AGGACTGCGG CCCCGCGTGG 
CGCGCGGCCC CCGCGGCCTA TGACGCGTCT GACACGCACC TGCAAATCCT GGGCAAGCCA 
GTGATGGAGC GTTGGGAGAC CCCCTATATG CATGCGCTAG CGGCGGCTGC TGCCTCCAGA 
GGGGGCCGGG TCTTGGAAGT GGGCTTCGGT ATGGCCATTG CAGCCTCCAG GGTGCAACAG 
GCCCCCATAG AGGAACACTG GATTATTGAG TGCAATGATG GGGTCTTCCA GCGTCTACAA 
GACTGGGCCC TGCGGCAGCC ACATAAGGTT GTTCCCTTGA AAGGCCTGTG GGAGGAGGTG 
GCACCTACCC TGCCTGACGG TCACTTTGAT GGGATTCTAT ATGACACGTA CCCGCTGTCT 
GAAGAGGCCT GGCACACTCA CCAGTTCAAC TTTATTAAGA ATCATGCCTT CCGCTTGCTG 
AAGACCGGGG GCGTCCTCAC CTACTGCAAC CTCACGTCCT GGGGGGAGCT CATGAAGTCC 
AAATACACAG ACATCACCAC CATGTTTGAG GAGACGCAGG TGCCTGCACT GCAGGAAGCT 
GGCTTCCTGA AAGAAAACAT CTGCACAGAG GTGATGGCAC TGGTGCCCCC AGCCGACTGC 
CGCTACTATG CCTTCCCTCA GATGATCACA CCCCTGGTCA CCAAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gamt ( XM_006513222.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513285.1 Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu44203
Clone ID Related Accession (Same CDS sequence)	XM_006513222.3 , XM_006513222.4
Accession Version	XM_006513222.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	guanidinoacetate N-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513222.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCTT CTGCAGCTAG CCCGCTCTTC GCGCCCGGCG AGGACTGCGG CCCCGCGTGG 
CGCGCGGCCC CCGCGGCCTA TGACGCGTCT GACACGCACC TGCAAATCCT GGGCAAGCCA 
GTGATGGAGC GTTGGGAGAC CCCCTATATG CATGCGCTAG CGGCGGCTGC TGCCTCCAGA 
GGGGGCCGGG TCTTGGAAGT GGGCTTCGGT ATGGCCATTG CAGCCTCCAG GGTGCAACAG 
GCCCCCATAG AGGAACACTG GATTATTGAG TGCAATGATG GGGTCTTCCA GCGTCTACAA 
GACTGGGCCC TGCGGCAGCC ACATAAGGTT GTTCCCTTGA AAGGCCTGTG GGAGGAGGTG 
GCACCTACCC TGCCTGACGG TCACTTTGAT GGGATTCTAT ATGACACGTA CCCGCTGTCT 
GAAGAGGCCT GGCACACTCA CCAGTTCAAC TTTATTAAGC TCTCTTCCCA TGGTCCTACC 
CCTTCCTGTC CACTGGCCTC TCTGCAGAAT CATGCCTTCC GCTTGCTGAA GACCGGGGGC 
GTCCTCACCT ACTGCAACCT CACGTCCTGG GGGGAGCTCA TGAAGTCCAA ATACACAGAC 
ATCACCACCA TGTTTGAGGT CCTGTCCCAC ATCATCTGGG GTGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513285.1
CDS	125..775
Translation	MSSSAASPLFAPGEDCGPAWRAAPAAYDASDTHLQILGKPVMERWETPYMHALAAAAASRGGRVLEVGFGMAIAASRVQQAPIEEHWIIECNDGVFQRLQDWALRQPHKVVPLKGLWEEVAPTLPDGHFDGILYDTYPLSEEAWHTHQFNFIKLSSHGPTPSCPLASLQNHAFRLLKTGGVLTYCNLTSWGELMKSKYTDITTMFEVLSHIIWGDL

Target ORF information:

RefSeq Version	XM_006513222.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513222.4

ORF Insert Sequence:

ATGAGCTCTT CTGCAGCTAG CCCGCTCTTC GCGCCCGGCG AGGACTGCGG CCCCGCGTGG 
CGCGCGGCCC CCGCGGCCTA TGACGCGTCT GACACGCACC TGCAAATCCT GGGCAAGCCA 
GTGATGGAGC GTTGGGAGAC CCCCTATATG CATGCGCTAG CGGCGGCTGC TGCCTCCAGA 
GGGGGCCGGG TCTTGGAAGT GGGCTTCGGT ATGGCCATTG CAGCCTCCAG GGTGCAACAG 
GCCCCCATAG AGGAACACTG GATTATTGAG TGCAATGATG GGGTCTTCCA GCGTCTACAA 
GACTGGGCCC TGCGGCAGCC ACATAAGGTT GTTCCCTTGA AAGGCCTGTG GGAGGAGGTG 
GCACCTACCC TGCCTGACGG TCACTTTGAT GGGATTCTAT ATGACACGTA CCCGCTGTCT 
GAAGAGGCCT GGCACACTCA CCAGTTCAAC TTTATTAAGC TCTCTTCCCA TGGTCCTACC 
CCTTCCTGTC CACTGGCCTC TCTGCAGAAT CATGCCTTCC GCTTGCTGAA GACCGGGGGC 
GTCCTCACCT ACTGCAACCT CACGTCCTGG GGGGAGCTCA TGAAGTCCAA ATACACAGAC 
ATCACCACCA TGTTTGAGGT CCTGTCCCAC ATCATCTGGG GTGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gamt ( NM_001347119.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334048.1 Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu44202
Clone ID Related Accession (Same CDS sequence)	NM_001347119.1
Accession Version	NM_001347119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-18
Organism	Mus musculus(house mouse)
Product	guanidinoacetate N-methyltransferase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA222404.1, AC140229.3, AK140688.1 and BE992264.1. On Nov 16, 2016 this sequence version replaced XM_006513221.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2), has the same N- and C-termini, but is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK140688.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCTT CTGCAGCTAG CCCGCTCTTC GCGCCCGGCG AGGACTGCGG CCCCGCGTGG 
CGCGCGGCCC CCGCGGCCTA TGACGCGTCT GACACGCACC TGCAAATCCT GGGCAAGCCA 
GTGATGGAGC GTTGGGAGAC CCCCTATATG CATGCGCTAG CGGCGGCTGC TGCCTCCAGA 
GGGGGCCGGG TCTTGGAAGT GGGCTTCGGT ATGGCCATTG CAGCCTCCAG GGTGCAACAG 
GCCCCCATAG AGGAACACTG GATTATTGAG TGCAATGATG GGGTCTTCCA GCGTCTACAA 
GACTGGGCCC TGCGGCAGCC ACATAAGGTT GTTCCCTTGA AAGGCCTGTG GGAGGAGGTG 
GCACCTACCC TGCCTGACGG TCACTTTGAT GGGATTCTAT ATGACACGTA CCCGCTGTCT 
GAAGAGGCCT GGCACACTCA CCAGTTCAAC TTTATTAAGC TCTCTTCCCA TGGTCCTACC 
CCTTCCTGTC CACTGGCCTC TCTGCAGAAT CATGCCTTCC GCTTGCTGAA GACCGGGGGC 
GTCCTCACCT ACTGCAACCT CACGTCCTGG GGGGAGCTCA TGAAGTCCAA ATACACAGAC 
ATCACCACCA TGTTTGAGGA GACGCAGGTG CCTGCACTGC AGGAAGCTGG CTTCCTGAAA 
GAAAACATCT GCACAGAGGT GATGGCACTG GTGCCCCCAG CCGACTGCCG CTACTATGCC 
TTCCCTCAGA TGATCACACC CCTGGTCACC AAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334048.1
CDS	112..870
Translation	MSSSAASPLFAPGEDCGPAWRAAPAAYDASDTHLQILGKPVMERWETPYMHALAAAAASRGGRVLEVGFGMAIAASRVQQAPIEEHWIIECNDGVFQRLQDWALRQPHKVVPLKGLWEEVAPTLPDGHFDGILYDTYPLSEEAWHTHQFNFIKLSSHGPTPSCPLASLQNHAFRLLKTGGVLTYCNLTSWGELMKSKYTDITTMFEETQVPALQEAGFLKENICTEVMALVPPADCRYYAFPQMITPLVTKH

Target ORF information:

RefSeq Version	NM_001347119.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347119.1

ORF Insert Sequence:

ATGAGCTCTT CTGCAGCTAG CCCGCTCTTC GCGCCCGGCG AGGACTGCGG CCCCGCGTGG 
CGCGCGGCCC CCGCGGCCTA TGACGCGTCT GACACGCACC TGCAAATCCT GGGCAAGCCA 
GTGATGGAGC GTTGGGAGAC CCCCTATATG CATGCGCTAG CGGCGGCTGC TGCCTCCAGA 
GGGGGCCGGG TCTTGGAAGT GGGCTTCGGT ATGGCCATTG CAGCCTCCAG GGTGCAACAG 
GCCCCCATAG AGGAACACTG GATTATTGAG TGCAATGATG GGGTCTTCCA GCGTCTACAA 
GACTGGGCCC TGCGGCAGCC ACATAAGGTT GTTCCCTTGA AAGGCCTGTG GGAGGAGGTG 
GCACCTACCC TGCCTGACGG TCACTTTGAT GGGATTCTAT ATGACACGTA CCCGCTGTCT 
GAAGAGGCCT GGCACACTCA CCAGTTCAAC TTTATTAAGC TCTCTTCCCA TGGTCCTACC 
CCTTCCTGTC CACTGGCCTC TCTGCAGAAT CATGCCTTCC GCTTGCTGAA GACCGGGGGC 
GTCCTCACCT ACTGCAACCT CACGTCCTGG GGGGAGCTCA TGAAGTCCAA ATACACAGAC 
ATCACCACCA TGTTTGAGGA GACGCAGGTG CCTGCACTGC AGGAAGCTGG CTTCCTGAAA 
GAAAACATCT GCACAGAGGT GATGGCACTG GTGCCCCCAG CCGACTGCCG CTACTATGCC 
TTCCCTCAGA TGATCACACC CCTGGTCACC AAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gamt ( XM_006513222.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513285.1 Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu44203
Clone ID Related Accession (Same CDS sequence)	XM_006513222.3 , XM_006513222.4
Accession Version	XM_006513222.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	guanidinoacetate N-methyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCTT CTGCAGCTAG CCCGCTCTTC GCGCCCGGCG AGGACTGCGG CCCCGCGTGG 
CGCGCGGCCC CCGCGGCCTA TGACGCGTCT GACACGCACC TGCAAATCCT GGGCAAGCCA 
GTGATGGAGC GTTGGGAGAC CCCCTATATG CATGCGCTAG CGGCGGCTGC TGCCTCCAGA 
GGGGGCCGGG TCTTGGAAGT GGGCTTCGGT ATGGCCATTG CAGCCTCCAG GGTGCAACAG 
GCCCCCATAG AGGAACACTG GATTATTGAG TGCAATGATG GGGTCTTCCA GCGTCTACAA 
GACTGGGCCC TGCGGCAGCC ACATAAGGTT GTTCCCTTGA AAGGCCTGTG GGAGGAGGTG 
GCACCTACCC TGCCTGACGG TCACTTTGAT GGGATTCTAT ATGACACGTA CCCGCTGTCT 
GAAGAGGCCT GGCACACTCA CCAGTTCAAC TTTATTAAGC TCTCTTCCCA TGGTCCTACC 
CCTTCCTGTC CACTGGCCTC TCTGCAGAAT CATGCCTTCC GCTTGCTGAA GACCGGGGGC 
GTCCTCACCT ACTGCAACCT CACGTCCTGG GGGGAGCTCA TGAAGTCCAA ATACACAGAC 
ATCACCACCA TGTTTGAGGT CCTGTCCCAC ATCATCTGGG GTGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513285.1
CDS	126..776
Translation	MSSSAASPLFAPGEDCGPAWRAAPAAYDASDTHLQILGKPVMERWETPYMHALAAAAASRGGRVLEVGFGMAIAASRVQQAPIEEHWIIECNDGVFQRLQDWALRQPHKVVPLKGLWEEVAPTLPDGHFDGILYDTYPLSEEAWHTHQFNFIKLSSHGPTPSCPLASLQNHAFRLLKTGGVLTYCNLTSWGELMKSKYTDITTMFEVLSHIIWGDL

Target ORF information:

RefSeq Version	XM_006513222.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus guanidinoacetate methyltransferase (Gamt), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513222.3

ORF Insert Sequence:

ATGAGCTCTT CTGCAGCTAG CCCGCTCTTC GCGCCCGGCG AGGACTGCGG CCCCGCGTGG 
CGCGCGGCCC CCGCGGCCTA TGACGCGTCT GACACGCACC TGCAAATCCT GGGCAAGCCA 
GTGATGGAGC GTTGGGAGAC CCCCTATATG CATGCGCTAG CGGCGGCTGC TGCCTCCAGA 
GGGGGCCGGG TCTTGGAAGT GGGCTTCGGT ATGGCCATTG CAGCCTCCAG GGTGCAACAG 
GCCCCCATAG AGGAACACTG GATTATTGAG TGCAATGATG GGGTCTTCCA GCGTCTACAA 
GACTGGGCCC TGCGGCAGCC ACATAAGGTT GTTCCCTTGA AAGGCCTGTG GGAGGAGGTG 
GCACCTACCC TGCCTGACGG TCACTTTGAT GGGATTCTAT ATGACACGTA CCCGCTGTCT 
GAAGAGGCCT GGCACACTCA CCAGTTCAAC TTTATTAAGC TCTCTTCCCA TGGTCCTACC 
CCTTCCTGTC CACTGGCCTC TCTGCAGAAT CATGCCTTCC GCTTGCTGAA GACCGGGGGC 
GTCCTCACCT ACTGCAACCT CACGTCCTGG GGGGAGCTCA TGAAGTCCAA ATACACAGAC 
ATCACCACCA TGTTTGAGGT CCTGTCCCAC ATCATCTGGG GTGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cell-Type-Specific Spatiotemporal Expression of Creatine Biosynthetic Enzyme S-adenosylmethionine:guanidinoacetate N-methyltransferase in Developing Mouse Brain.
Neurochemical research(2017 Dec)
Tachikawa M,Watanabe M,Fukaya M,Sakai K,Terasaki T,Hosoya KI

Creatine Enhances Mitochondrial-Mediated Oligodendrocyte Survival After Demyelinating Injury.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(6)1479-1492(2017 Feb)
Chamberlain KA,Chapey KS,Nanescu SE,Huang JK

Unchanged mitochondrial organization and compartmentation of high-energy phosphates in creatine-deficient GAMT-/- mouse hearts.
American journal of physiology. Heart and circulatory physiology305(4)H506-20(2013 Aug)
Branovets J,Sepp M,Kotlyarova S,Jepihhina N,Sokolova N,Aksentijevic D,Lygate CA,Neubauer S,Vendelin M,Birkedal R

A role for thioredoxin-interacting protein (Txnip) in cellular creatine homeostasis.
American journal of physiology. Endocrinology and metabolism305(2)E263-70(2013 Jul)
Zervou S,Ray T,Sahgal N,Sebag-Montefiore L,Cross R,Medway DJ,Ostrowski PJ,Neubauer S,Lygate CA

Living without creatine: unchanged exercise capacity and response to chronic myocardial infarction in creatine-deficient mice.
Circulation research112(6)945-55(2013 Mar)
Lygate CA,Aksentijevic D,Dawson D,ten Hove M,Phillips D,de Bono JP,Medway DJ,Sebag-Montefiore L,Hunyor I,Channon KM,Clarke K,Zervou S,Watkins H,Balaban RS,Neubauer S

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Gamt cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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