Gaa cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gaa
Entrez Gene ID	14387
Full Name	glucosidase, alpha, acid
Synonyms	E430018M07Rik
General protein information	Preferred Names glucosidase, alpha, acid Names lysosomal alpha-glucosidase acid maltase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 E2\|11 83.35 cM
Summary	This gene encodes a lysosomal acid glucosidase that is involved in the degradation of glycogen. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that cleaves alpha-1-4 and alpha-1-6 glycosidic bonds of glycogen, maltose and intermediate oligosaccharides within the lysosome. Mice lacking the encoded protein exhibit symptoms similar to human Pompe syndrome such as accumulation of glycogen in cardiac and skeletal muscle lysosomes resulting in reduced mobility and strength. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008064.4	NP_032090.3	lysosomal alpha-glucosidase preproprotein
NM_001159324.2	NP_001152796.1	lysosomal alpha-glucosidase preproprotein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC422082	XP_420085.4
Schizosaccharomyces pombe (fission yeast)	SPAC30D11.01c	NP_593216.2
Canis lupus familiaris (dog)	GAA	XP_005624079.1
Mus musculus (house mouse)	Gaa	NP_032090.3
Pan troglodytes (chimpanzee)	GAA	XP_001160653.1
Rattus norvegicus (Norway rat)	Gaa	NP_954549.1
Danio rerio (zebrafish)	gaa	XP_001921957.2
Schizosaccharomyces pombe (fission yeast)	agl1	NP_593996.1
Oryza sativa (rice)	Os01g0130400	NP_001041922.1
Bos taurus (cattle)	GAA	NP_776338.1
Schizosaccharomyces pombe (fission yeast)	SPAC1039.11c	XP_001713119.1
Neurospora crassa	NCU09281	XP_958500.1
Xenopus tropicalis (tropical clawed frog)	gaa	XP_002932561.2
Homo sapiens (human)	GAA	NP_001073272.1
	GAA	XP_001109980.1
Magnaporthe oryzae (rice blast fungus)	MGG_10662	XP_003710096.1
Arabidopsis thaliana (thale cress)	XYL1	NP_177023.1
Arabidopsis thaliana (thale cress)	AT3G45940	NP_190180.1

Gene Ontology
Bibliography

Related articles in PubMed

Rescue of Pompe disease in mice by AAV-mediated liver delivery of secretable acid ?-glucosidase.
Puzzo F, Colella P, Biferi MG, Bali D, Paulk NK, Vidal P, Collaud F, Simon-Sola M, Charles S, Hardet R, Leborgne C, Meliani A, Cohen-Tannoudji M, Astord S, Gjata B, Sellier P, van Wittenberghe L, Vignaud A, Boisgerault F, Barkats M, Laforet P, Kay MA, Koeberl DD, Ronzitti G, Mingozzi F
Science translational medicine9(418)(2017 Nov)

AAV-mediated transcription factor EB (TFEB) gene delivery ameliorates muscle pathology and function in the murine model of Pompe Disease.
Gatto F, Rossi B, Tarallo A, Polishchuk E, Polishchuk R, Carrella A, Nusco E, Alvino FG, Iacobellis F, De Leonibus E, Auricchio A, Diez-Roux G, Ballabio A, Parenti G
Scientific reports7(1)15089(2017 Nov)

Airway smooth muscle dysfunction in Pompe (
Keeler AM, Liu D, Zieger M, Xiong L, Salemi J, Bellv? K, Byrne BJ, Fuller DD, ZhuGe R, ElMallah MK
American journal of physiology. Lung cellular and molecular physiology312(6)L873-L881(2017 Jun)

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Transcriptome assessment of the Pompe (Gaa-/-) mouse spinal cord indicates widespread neuropathology.
Turner SMF, Falk DJ, Byrne BJ, Fuller DD
Physiological genomics48(11)785-794(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GAA enzyme deficiency leads to glycogen accumulation in the trachea and bronchi and impairs the ability of lower airway smooth muscle to regulate calcium and respond appropriately to bronchodilator or constrictors.
Title: Airway smooth muscle dysfunction in Pompe (

GAA deficiency results in reduced mTORC1 activation that is partly responsible for the skeletal muscle wasting phenotype and can be amerliorated by leucine supplementation.
Title: Suppression of mTORC1 activation in acid-?-glucosidase-deficient cells and mice is ameliorated by leucine supplementation.

slow glucose release can be induced through the toggling of activities of the mucosal alpha-glucosidases by selective enzyme inhibition
Title: Modulation of starch digestion for slow glucose release through "toggling" of activities of mucosal ?-glucosidases.

Results describe the inhibitory effects of Chana series chalcone derivatives on the activities of alpha-glucosidase and DPP-4, and on adipocyte differentiation.
Title: Effects of novel chalcone derivatives on ?-glucosidase, dipeptidyl peptidase-4, and adipocyte differentiation in vitro.

These studies suggest that hyase enhances penetration of enzyme into the tissues including muscle during ERT and therefore hyase pretreatment may be important in treating Pompe disease.
Title: Hyaluronidase increases the biodistribution of acid alpha-1,4 glucosidase in the muscle of Pompe disease mice: an approach to enhance the efficacy of enzyme replacement therapy.

The following Gaa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gaa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01311	NM_008064.4 Latest version!	Mus musculus glucosidase, alpha, acid (Gaa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00
OMu01311	NM_001159324.2 Latest version!	Mus musculus glucosidase, alpha, acid (Gaa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gaa ( NM_008064.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032090.3 Mus musculus glucosidase, alpha, acid (Gaa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01311
Clone ID Related Accession (Same CDS sequence)	NM_008064.4 , NM_001159324.2
Accession Version	NM_008064.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	lysosomal alpha-glucosidase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB839328.1, AL672140.7, AK146538.1, AK052211.1 and BP767732.1. On Dec 1, 2015 this sequence version replaced NM_008064.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010210.1, AK052211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATATAC GGAAGCCCCT CTGTTCGAAC TCCGTGGTTG GGGCCTGCAC CCTTATCTCT 
CTGACTACAG CGGTCATCCT GGGTCATCTC ATGCTTCGGG AGTTAATGCT GCTTCCCCAA 
GACCTTCATG AGTCCTCTTC AGGACTGTGG AAGACGTACC GACCTCACCA CCAGGAAGGT 
TACAAGCCAG GGCCTCTGCA CATCCAGGAG CAGACTGAAC AGCCCAAAGA AGCACCCACA 
CAGTGTGATG TGCCCCCCAG CAGCCGCTTT GACTGTGCCC CCGACAAAGG CATCTCACAG 
GAGCAATGCG AGGCCCGCGG CTGCTGCTAT GTCCCAGCAG GGCAGGTGCT GAAGGAGCCG 
CAGATAGGGC AGCCCTGGTG TTTCTTCCCT CCCAGCTACC CAAGCTACCG TCTAGAGAAC 
CTGAGCTCTA CAGAGTCGGG GTACACAGCC ACCCTGACCC GTACCAGCCC GACCTTCTTC 
CCAAAGGATG TGCTGACCTT ACAGCTGGAG GTGCTGATGG AGACAGACAG CCGCCTCCAC 
TTCAAGATCA AAGATCCTGC TAGTAAGCGC TACGAAGTGC CCCTGGAGAC CCCACGTGTG 
CTGAGCCAGG CACCATCCCC ACTTTACAGC GTGGAATTCT CAGAGGAACC CTTTGGAGTG 
ATCGTTCGTA GGAAGCTTGG TGGCCGAGTG TTGCTGAACA CAACCGTGGC CCCCCTGTTC 
TTCGCTGACC AGTTCCTGCA GCTGTCCACT TCCCTGCCCT CCCAGCACAT CACAGGCCTG 
GGGGAACACC TCAGCCCACT CATGCTCAGC ACCGACTGGG CTCGTATCAC CCTCTGGAAC 
CGGGACACAC CACCCTCGCA AGGTACCAAC CTCTACGGGT CACATCCTTT CTACCTGGCA 
CTGGAGGACG GTGGCTTGGC TCACGGTGTC TTCTTGCTAA ACAGCAATGC CATGGATGTC 
ATCCTGCAAC CCAGCCCAGC CCTAACCTGG AGGTCAACGG GCGGGATCCT GGATGTGTAT 
GTGTTCCTAG GCCCAGAGCC CAAGAGCGTT GTGCAACAAT ACCTGGATGT TGTGGGATAC 
CCCTTCATGC CTCCATACTG GGGCCTCGGC TTCCACCTCT GCCGCTGGGG CTACTCCTCG 
ACCGCCATTG TCCGCCAGGT AGTGGAGAAC ATGACCAGGA CACACTTCCC GCTGGACGTG 
CAATGGAATG ACCTGGACTA CATGGACGCC CGAAGAGACT TCACCTTCAA CCAGGACAGC 
TTTGCCGACT TCCCAGACAT GGTGCGGGAG CTGCACCAGG ATGGCCGGCG CTACATGATG 
ATCGTGGACC CTGCCATCAG CAGCGCAGGC CCTGCTGGGA GTTACAGGCC CTACGACGAG 
GGTCTGCGGA GGGGCGTGTT CATCACCAAC GAGACTGGGC AGCCGCTGAT TGGGAAGGTT 
TGGCCTGGAA CCACCGCCTT CCCTGATTTC ACCAACCCTG AGACCCTTGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA CGCCCAGGTG CCCTTCGATG GCATGTGGCT CGACATGAAC 
GAACCGTCCA ACTTCGTTAG AGGCTCTCAG CAGGGCTGCC CCAACAATGA ACTGGAGAAC 
CCCCCCTATG TGCCCGGGGT GGTTGGCGGG ATCTTGCAGG CAGCCACCAT CTGTGCCTCC 
AGCCACCAAT TCCTCTCCAC ACACTACAAC CTCCACAACC TGTACGGCCT CACTGAAGCT 
ATCGCCTCCA GCAGGGCCCT GGTCAAGACT CGGGGAACAC GACCCTTTGT GATCTCCCGC 
TCAACCTTCT CGGGCCACGG CCGGTACGCT GGTCACTGGA CAGGGGATGT GCGGAGCTCT 
TGGGAGCATC TTGCATACTC TGTGCCAGAC ATCCTGCAGT TCAACCTGCT GGGCGTGCCC 
CTGGTCGGGG CGGACATCTG CGGCTTCATA GGAGACACGT CAGAAGAGCT GTGTGTGCGC 
TGGACCCAGT TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAATGACCT GAATAGCGTG 
CCTCAGGAGC CGTACAGGTT CAGCGAGACG GCGCAGCAGG CCATGAGGAA GGCCTTCGCC 
TTACGCTATG CCCTTCTGCC CTACCTGTAC ACTCTCTTCC ACCGCGCCCA CGTCAGAGGA 
GACACGGTGG CCCGGCCCCT CTTCCTGGAG TTCCCTGAGG ATCCCAGCAC CTGGTCTGTG 
GACCGCCAGC TCTTGTGGGG GCCGGCCCTG CTCATCACAC CTGTGCTTGA GCCTGGGAAA 
ACTGAAGTGA CGGGCTACTT CCCCAAGGGC ACGTGGTACA ACATGCAGAT GGTGTCAGTG 
GATTCCCTCG GTACTCTCCC TTCTCCATCA TCGGCTTCAT CCTTCAGATC TGCTGTCCAG 
AGCAAGGGGC AGTGGCTGAC ACTGGAAGCC CCACTGGATA CCATCAACGT GCACCTGAGG 
GAGGGGTACA TCATACCGCT GCAGGGTCCC AGCCTCACAA CCACGGAGTC CCGAAAGCAG 
CCCATGGCTC TGGCTGTGGC ATTAACAGCA AGCGGCGAGG CCGATGGGGA GCTGTTCTGG 
GACGACGGGG AGAGCCTCGC GGTTCTGGAG CGTGGGGCCT ACACACTGGT CACCTTCTCA 
GCCAAGAACA ATACCATTGT GAACAAGTTA GTGCGTGTGA CCAAGGAGGG AGCTGAGCTA 
CAACTGAGGG AGGTGACCGT CTTGGGAGTG GCCACAGCTC CTACCCAGGT CCTTTCCAAC 
GGCATCCCTG TCTCCAATTT CACCTACAGC CCTGACAACA AGAGCCTGGC CATCCCTGTC 
TCACTGCTGA TGGGAGAGCT GTTTCAAATC AGCTGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032090.3
CDS	306..3167
Translation	MNIRKPLCSNSVVGACTLISLTTAVILGHLMLRELMLLPQDLHESSSGLWKTYRPHHQEGYKPGPLHIQEQTEQPKEAPTQCDVPPSSRFDCAPDKGISQEQCEARGCCYVPAGQVLKEPQIGQPWCFFPPSYPSYRLENLSSTESGYTATLTRTSPTFFPKDVLTLQLEVLMETDSRLHFKIKDPASKRYEVPLETPRVLSQAPSPLYSVEFSEEPFGVIVRRKLGGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLGEHLSPLMLSTDWARITLWNRDTPPSQGTNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVILQPSPALTWRSTGGILDVYVFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDSFADFPDMVRELHQDGRRYMMIVDPAISSAGPAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGTTAFPDFTNPETLDWWQDMVSEFHAQVPFDGMWLDMNEPSNFVRGSQQGCPNNELENPPYVPGVVGGILQAATICASSHQFLSTHYNLHNLYGLTEAIASSRALVKTRGTRPFVISRSTFSGHGRYAGHWTGDVRSSWEHLAYSVPDILQFNLLGVPLVGADICGFIGDTSEELCVRWTQLGAFYPFMRNHNDLNSVPQEPYRFSETAQQAMRKAFALRYALLPYLYTLFHRAHVRGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLITPVLEPGKTEVTGYFPKGTWYNMQMVSVDSLGTLPSPSSASSFRSAVQSKGQWLTLEAPLDTINVHLREGYIIPLQGPSLTTTESRKQPMALAVALTASGEADGELFWDDGESLAVLERGAYTLVTFSAKNNTIVNKLVRVTKEGAELQLREVTVLGVATAPTQVLSNGIPVSNFTYSPDNKSLAIPVSLLMGELFQISWS

Target ORF information:

RefSeq Version	NM_008064.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus glucosidase, alpha, acid (Gaa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008064.4

ORF Insert Sequence:

ATGAATATAC GGAAGCCCCT CTGTTCGAAC TCCGTGGTTG GGGCCTGCAC CCTTATCTCT 
CTGACTACAG CGGTCATCCT GGGTCATCTC ATGCTTCGGG AGTTAATGCT GCTTCCCCAA 
GACCTTCATG AGTCCTCTTC AGGACTGTGG AAGACGTACC GACCTCACCA CCAGGAAGGT 
TACAAGCCAG GGCCTCTGCA CATCCAGGAG CAGACTGAAC AGCCCAAAGA AGCACCCACA 
CAGTGTGATG TGCCCCCCAG CAGCCGCTTT GACTGTGCCC CCGACAAAGG CATCTCACAG 
GAGCAATGCG AGGCCCGCGG CTGCTGCTAT GTCCCAGCAG GGCAGGTGCT GAAGGAGCCG 
CAGATAGGGC AGCCCTGGTG TTTCTTCCCT CCCAGCTACC CAAGCTACCG TCTAGAGAAC 
CTGAGCTCTA CAGAGTCGGG GTACACAGCC ACCCTGACCC GTACCAGCCC GACCTTCTTC 
CCAAAGGATG TGCTGACCTT ACAGCTGGAG GTGCTGATGG AGACAGACAG CCGCCTCCAC 
TTCAAGATCA AAGATCCTGC TAGTAAGCGC TACGAAGTGC CCCTGGAGAC CCCACGTGTG 
CTGAGCCAGG CACCATCCCC ACTTTACAGC GTGGAATTCT CAGAGGAACC CTTTGGAGTG 
ATCGTTCGTA GGAAGCTTGG TGGCCGAGTG TTGCTGAACA CAACCGTGGC CCCCCTGTTC 
TTCGCTGACC AGTTCCTGCA GCTGTCCACT TCCCTGCCCT CCCAGCACAT CACAGGCCTG 
GGGGAACACC TCAGCCCACT CATGCTCAGC ACCGACTGGG CTCGTATCAC CCTCTGGAAC 
CGGGACACAC CACCCTCGCA AGGTACCAAC CTCTACGGGT CACATCCTTT CTACCTGGCA 
CTGGAGGACG GTGGCTTGGC TCACGGTGTC TTCTTGCTAA ACAGCAATGC CATGGATGTC 
ATCCTGCAAC CCAGCCCAGC CCTAACCTGG AGGTCAACGG GCGGGATCCT GGATGTGTAT 
GTGTTCCTAG GCCCAGAGCC CAAGAGCGTT GTGCAACAAT ACCTGGATGT TGTGGGATAC 
CCCTTCATGC CTCCATACTG GGGCCTCGGC TTCCACCTCT GCCGCTGGGG CTACTCCTCG 
ACCGCCATTG TCCGCCAGGT AGTGGAGAAC ATGACCAGGA CACACTTCCC GCTGGACGTG 
CAATGGAATG ACCTGGACTA CATGGACGCC CGAAGAGACT TCACCTTCAA CCAGGACAGC 
TTTGCCGACT TCCCAGACAT GGTGCGGGAG CTGCACCAGG ATGGCCGGCG CTACATGATG 
ATCGTGGACC CTGCCATCAG CAGCGCAGGC CCTGCTGGGA GTTACAGGCC CTACGACGAG 
GGTCTGCGGA GGGGCGTGTT CATCACCAAC GAGACTGGGC AGCCGCTGAT TGGGAAGGTT 
TGGCCTGGAA CCACCGCCTT CCCTGATTTC ACCAACCCTG AGACCCTTGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA CGCCCAGGTG CCCTTCGATG GCATGTGGCT CGACATGAAC 
GAACCGTCCA ACTTCGTTAG AGGCTCTCAG CAGGGCTGCC CCAACAATGA ACTGGAGAAC 
CCCCCCTATG TGCCCGGGGT GGTTGGCGGG ATCTTGCAGG CAGCCACCAT CTGTGCCTCC 
AGCCACCAAT TCCTCTCCAC ACACTACAAC CTCCACAACC TGTACGGCCT CACTGAAGCT 
ATCGCCTCCA GCAGGGCCCT GGTCAAGACT CGGGGAACAC GACCCTTTGT GATCTCCCGC 
TCAACCTTCT CGGGCCACGG CCGGTACGCT GGTCACTGGA CAGGGGATGT GCGGAGCTCT 
TGGGAGCATC TTGCATACTC TGTGCCAGAC ATCCTGCAGT TCAACCTGCT GGGCGTGCCC 
CTGGTCGGGG CGGACATCTG CGGCTTCATA GGAGACACGT CAGAAGAGCT GTGTGTGCGC 
TGGACCCAGT TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAATGACCT GAATAGCGTG 
CCTCAGGAGC CGTACAGGTT CAGCGAGACG GCGCAGCAGG CCATGAGGAA GGCCTTCGCC 
TTACGCTATG CCCTTCTGCC CTACCTGTAC ACTCTCTTCC ACCGCGCCCA CGTCAGAGGA 
GACACGGTGG CCCGGCCCCT CTTCCTGGAG TTCCCTGAGG ATCCCAGCAC CTGGTCTGTG 
GACCGCCAGC TCTTGTGGGG GCCGGCCCTG CTCATCACAC CTGTGCTTGA GCCTGGGAAA 
ACTGAAGTGA CGGGCTACTT CCCCAAGGGC ACGTGGTACA ACATGCAGAT GGTGTCAGTG 
GATTCCCTCG GTACTCTCCC TTCTCCATCA TCGGCTTCAT CCTTCAGATC TGCTGTCCAG 
AGCAAGGGGC AGTGGCTGAC ACTGGAAGCC CCACTGGATA CCATCAACGT GCACCTGAGG 
GAGGGGTACA TCATACCGCT GCAGGGTCCC AGCCTCACAA CCACGGAGTC CCGAAAGCAG 
CCCATGGCTC TGGCTGTGGC ATTAACAGCA AGCGGCGAGG CCGATGGGGA GCTGTTCTGG 
GACGACGGGG AGAGCCTCGC GGTTCTGGAG CGTGGGGCCT ACACACTGGT CACCTTCTCA 
GCCAAGAACA ATACCATTGT GAACAAGTTA GTGCGTGTGA CCAAGGAGGG AGCTGAGCTA 
CAACTGAGGG AGGTGACCGT CTTGGGAGTG GCCACAGCTC CTACCCAGGT CCTTTCCAAC 
GGCATCCCTG TCTCCAATTT CACCTACAGC CCTGACAACA AGAGCCTGGC CATCCCTGTC 
TCACTGCTGA TGGGAGAGCT GTTTCAAATC AGCTGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gaa ( NM_001159324.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152796.1 Mus musculus glucosidase, alpha, acid (Gaa), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01311
Clone ID Related Accession (Same CDS sequence)	NM_008064.4 , NM_001159324.2
Accession Version	NM_001159324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	lysosomal alpha-glucosidase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB839328.1, AL672140.7, AK146538.1 and BP767732.1. On Dec 1, 2015 this sequence version replaced NM_001159324.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146538.1, U49351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATATAC GGAAGCCCCT CTGTTCGAAC TCCGTGGTTG GGGCCTGCAC CCTTATCTCT 
CTGACTACAG CGGTCATCCT GGGTCATCTC ATGCTTCGGG AGTTAATGCT GCTTCCCCAA 
GACCTTCATG AGTCCTCTTC AGGACTGTGG AAGACGTACC GACCTCACCA CCAGGAAGGT 
TACAAGCCAG GGCCTCTGCA CATCCAGGAG CAGACTGAAC AGCCCAAAGA AGCACCCACA 
CAGTGTGATG TGCCCCCCAG CAGCCGCTTT GACTGTGCCC CCGACAAAGG CATCTCACAG 
GAGCAATGCG AGGCCCGCGG CTGCTGCTAT GTCCCAGCAG GGCAGGTGCT GAAGGAGCCG 
CAGATAGGGC AGCCCTGGTG TTTCTTCCCT CCCAGCTACC CAAGCTACCG TCTAGAGAAC 
CTGAGCTCTA CAGAGTCGGG GTACACAGCC ACCCTGACCC GTACCAGCCC GACCTTCTTC 
CCAAAGGATG TGCTGACCTT ACAGCTGGAG GTGCTGATGG AGACAGACAG CCGCCTCCAC 
TTCAAGATCA AAGATCCTGC TAGTAAGCGC TACGAAGTGC CCCTGGAGAC CCCACGTGTG 
CTGAGCCAGG CACCATCCCC ACTTTACAGC GTGGAATTCT CAGAGGAACC CTTTGGAGTG 
ATCGTTCGTA GGAAGCTTGG TGGCCGAGTG TTGCTGAACA CAACCGTGGC CCCCCTGTTC 
TTCGCTGACC AGTTCCTGCA GCTGTCCACT TCCCTGCCCT CCCAGCACAT CACAGGCCTG 
GGGGAACACC TCAGCCCACT CATGCTCAGC ACCGACTGGG CTCGTATCAC CCTCTGGAAC 
CGGGACACAC CACCCTCGCA AGGTACCAAC CTCTACGGGT CACATCCTTT CTACCTGGCA 
CTGGAGGACG GTGGCTTGGC TCACGGTGTC TTCTTGCTAA ACAGCAATGC CATGGATGTC 
ATCCTGCAAC CCAGCCCAGC CCTAACCTGG AGGTCAACGG GCGGGATCCT GGATGTGTAT 
GTGTTCCTAG GCCCAGAGCC CAAGAGCGTT GTGCAACAAT ACCTGGATGT TGTGGGATAC 
CCCTTCATGC CTCCATACTG GGGCCTCGGC TTCCACCTCT GCCGCTGGGG CTACTCCTCG 
ACCGCCATTG TCCGCCAGGT AGTGGAGAAC ATGACCAGGA CACACTTCCC GCTGGACGTG 
CAATGGAATG ACCTGGACTA CATGGACGCC CGAAGAGACT TCACCTTCAA CCAGGACAGC 
TTTGCCGACT TCCCAGACAT GGTGCGGGAG CTGCACCAGG ATGGCCGGCG CTACATGATG 
ATCGTGGACC CTGCCATCAG CAGCGCAGGC CCTGCTGGGA GTTACAGGCC CTACGACGAG 
GGTCTGCGGA GGGGCGTGTT CATCACCAAC GAGACTGGGC AGCCGCTGAT TGGGAAGGTT 
TGGCCTGGAA CCACCGCCTT CCCTGATTTC ACCAACCCTG AGACCCTTGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA CGCCCAGGTG CCCTTCGATG GCATGTGGCT CGACATGAAC 
GAACCGTCCA ACTTCGTTAG AGGCTCTCAG CAGGGCTGCC CCAACAATGA ACTGGAGAAC 
CCCCCCTATG TGCCCGGGGT GGTTGGCGGG ATCTTGCAGG CAGCCACCAT CTGTGCCTCC 
AGCCACCAAT TCCTCTCCAC ACACTACAAC CTCCACAACC TGTACGGCCT CACTGAAGCT 
ATCGCCTCCA GCAGGGCCCT GGTCAAGACT CGGGGAACAC GACCCTTTGT GATCTCCCGC 
TCAACCTTCT CGGGCCACGG CCGGTACGCT GGTCACTGGA CAGGGGATGT GCGGAGCTCT 
TGGGAGCATC TTGCATACTC TGTGCCAGAC ATCCTGCAGT TCAACCTGCT GGGCGTGCCC 
CTGGTCGGGG CGGACATCTG CGGCTTCATA GGAGACACGT CAGAAGAGCT GTGTGTGCGC 
TGGACCCAGT TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAATGACCT GAATAGCGTG 
CCTCAGGAGC CGTACAGGTT CAGCGAGACG GCGCAGCAGG CCATGAGGAA GGCCTTCGCC 
TTACGCTATG CCCTTCTGCC CTACCTGTAC ACTCTCTTCC ACCGCGCCCA CGTCAGAGGA 
GACACGGTGG CCCGGCCCCT CTTCCTGGAG TTCCCTGAGG ATCCCAGCAC CTGGTCTGTG 
GACCGCCAGC TCTTGTGGGG GCCGGCCCTG CTCATCACAC CTGTGCTTGA GCCTGGGAAA 
ACTGAAGTGA CGGGCTACTT CCCCAAGGGC ACGTGGTACA ACATGCAGAT GGTGTCAGTG 
GATTCCCTCG GTACTCTCCC TTCTCCATCA TCGGCTTCAT CCTTCAGATC TGCTGTCCAG 
AGCAAGGGGC AGTGGCTGAC ACTGGAAGCC CCACTGGATA CCATCAACGT GCACCTGAGG 
GAGGGGTACA TCATACCGCT GCAGGGTCCC AGCCTCACAA CCACGGAGTC CCGAAAGCAG 
CCCATGGCTC TGGCTGTGGC ATTAACAGCA AGCGGCGAGG CCGATGGGGA GCTGTTCTGG 
GACGACGGGG AGAGCCTCGC GGTTCTGGAG CGTGGGGCCT ACACACTGGT CACCTTCTCA 
GCCAAGAACA ATACCATTGT GAACAAGTTA GTGCGTGTGA CCAAGGAGGG AGCTGAGCTA 
CAACTGAGGG AGGTGACCGT CTTGGGAGTG GCCACAGCTC CTACCCAGGT CCTTTCCAAC 
GGCATCCCTG TCTCCAATTT CACCTACAGC CCTGACAACA AGAGCCTGGC CATCCCTGTC 
TCACTGCTGA TGGGAGAGCT GTTTCAAATC AGCTGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152796.1
CDS	175..3036
Translation	MNIRKPLCSNSVVGACTLISLTTAVILGHLMLRELMLLPQDLHESSSGLWKTYRPHHQEGYKPGPLHIQEQTEQPKEAPTQCDVPPSSRFDCAPDKGISQEQCEARGCCYVPAGQVLKEPQIGQPWCFFPPSYPSYRLENLSSTESGYTATLTRTSPTFFPKDVLTLQLEVLMETDSRLHFKIKDPASKRYEVPLETPRVLSQAPSPLYSVEFSEEPFGVIVRRKLGGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLGEHLSPLMLSTDWARITLWNRDTPPSQGTNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVILQPSPALTWRSTGGILDVYVFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDSFADFPDMVRELHQDGRRYMMIVDPAISSAGPAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGTTAFPDFTNPETLDWWQDMVSEFHAQVPFDGMWLDMNEPSNFVRGSQQGCPNNELENPPYVPGVVGGILQAATICASSHQFLSTHYNLHNLYGLTEAIASSRALVKTRGTRPFVISRSTFSGHGRYAGHWTGDVRSSWEHLAYSVPDILQFNLLGVPLVGADICGFIGDTSEELCVRWTQLGAFYPFMRNHNDLNSVPQEPYRFSETAQQAMRKAFALRYALLPYLYTLFHRAHVRGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLITPVLEPGKTEVTGYFPKGTWYNMQMVSVDSLGTLPSPSSASSFRSAVQSKGQWLTLEAPLDTINVHLREGYIIPLQGPSLTTTESRKQPMALAVALTASGEADGELFWDDGESLAVLERGAYTLVTFSAKNNTIVNKLVRVTKEGAELQLREVTVLGVATAPTQVLSNGIPVSNFTYSPDNKSLAIPVSLLMGELFQISWS

Target ORF information:

RefSeq Version	NM_001159324.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glucosidase, alpha, acid (Gaa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159324.2

ORF Insert Sequence:

ATGAATATAC GGAAGCCCCT CTGTTCGAAC TCCGTGGTTG GGGCCTGCAC CCTTATCTCT 
CTGACTACAG CGGTCATCCT GGGTCATCTC ATGCTTCGGG AGTTAATGCT GCTTCCCCAA 
GACCTTCATG AGTCCTCTTC AGGACTGTGG AAGACGTACC GACCTCACCA CCAGGAAGGT 
TACAAGCCAG GGCCTCTGCA CATCCAGGAG CAGACTGAAC AGCCCAAAGA AGCACCCACA 
CAGTGTGATG TGCCCCCCAG CAGCCGCTTT GACTGTGCCC CCGACAAAGG CATCTCACAG 
GAGCAATGCG AGGCCCGCGG CTGCTGCTAT GTCCCAGCAG GGCAGGTGCT GAAGGAGCCG 
CAGATAGGGC AGCCCTGGTG TTTCTTCCCT CCCAGCTACC CAAGCTACCG TCTAGAGAAC 
CTGAGCTCTA CAGAGTCGGG GTACACAGCC ACCCTGACCC GTACCAGCCC GACCTTCTTC 
CCAAAGGATG TGCTGACCTT ACAGCTGGAG GTGCTGATGG AGACAGACAG CCGCCTCCAC 
TTCAAGATCA AAGATCCTGC TAGTAAGCGC TACGAAGTGC CCCTGGAGAC CCCACGTGTG 
CTGAGCCAGG CACCATCCCC ACTTTACAGC GTGGAATTCT CAGAGGAACC CTTTGGAGTG 
ATCGTTCGTA GGAAGCTTGG TGGCCGAGTG TTGCTGAACA CAACCGTGGC CCCCCTGTTC 
TTCGCTGACC AGTTCCTGCA GCTGTCCACT TCCCTGCCCT CCCAGCACAT CACAGGCCTG 
GGGGAACACC TCAGCCCACT CATGCTCAGC ACCGACTGGG CTCGTATCAC CCTCTGGAAC 
CGGGACACAC CACCCTCGCA AGGTACCAAC CTCTACGGGT CACATCCTTT CTACCTGGCA 
CTGGAGGACG GTGGCTTGGC TCACGGTGTC TTCTTGCTAA ACAGCAATGC CATGGATGTC 
ATCCTGCAAC CCAGCCCAGC CCTAACCTGG AGGTCAACGG GCGGGATCCT GGATGTGTAT 
GTGTTCCTAG GCCCAGAGCC CAAGAGCGTT GTGCAACAAT ACCTGGATGT TGTGGGATAC 
CCCTTCATGC CTCCATACTG GGGCCTCGGC TTCCACCTCT GCCGCTGGGG CTACTCCTCG 
ACCGCCATTG TCCGCCAGGT AGTGGAGAAC ATGACCAGGA CACACTTCCC GCTGGACGTG 
CAATGGAATG ACCTGGACTA CATGGACGCC CGAAGAGACT TCACCTTCAA CCAGGACAGC 
TTTGCCGACT TCCCAGACAT GGTGCGGGAG CTGCACCAGG ATGGCCGGCG CTACATGATG 
ATCGTGGACC CTGCCATCAG CAGCGCAGGC CCTGCTGGGA GTTACAGGCC CTACGACGAG 
GGTCTGCGGA GGGGCGTGTT CATCACCAAC GAGACTGGGC AGCCGCTGAT TGGGAAGGTT 
TGGCCTGGAA CCACCGCCTT CCCTGATTTC ACCAACCCTG AGACCCTTGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA CGCCCAGGTG CCCTTCGATG GCATGTGGCT CGACATGAAC 
GAACCGTCCA ACTTCGTTAG AGGCTCTCAG CAGGGCTGCC CCAACAATGA ACTGGAGAAC 
CCCCCCTATG TGCCCGGGGT GGTTGGCGGG ATCTTGCAGG CAGCCACCAT CTGTGCCTCC 
AGCCACCAAT TCCTCTCCAC ACACTACAAC CTCCACAACC TGTACGGCCT CACTGAAGCT 
ATCGCCTCCA GCAGGGCCCT GGTCAAGACT CGGGGAACAC GACCCTTTGT GATCTCCCGC 
TCAACCTTCT CGGGCCACGG CCGGTACGCT GGTCACTGGA CAGGGGATGT GCGGAGCTCT 
TGGGAGCATC TTGCATACTC TGTGCCAGAC ATCCTGCAGT TCAACCTGCT GGGCGTGCCC 
CTGGTCGGGG CGGACATCTG CGGCTTCATA GGAGACACGT CAGAAGAGCT GTGTGTGCGC 
TGGACCCAGT TGGGGGCCTT CTACCCCTTC ATGCGGAACC ACAATGACCT GAATAGCGTG 
CCTCAGGAGC CGTACAGGTT CAGCGAGACG GCGCAGCAGG CCATGAGGAA GGCCTTCGCC 
TTACGCTATG CCCTTCTGCC CTACCTGTAC ACTCTCTTCC ACCGCGCCCA CGTCAGAGGA 
GACACGGTGG CCCGGCCCCT CTTCCTGGAG TTCCCTGAGG ATCCCAGCAC CTGGTCTGTG 
GACCGCCAGC TCTTGTGGGG GCCGGCCCTG CTCATCACAC CTGTGCTTGA GCCTGGGAAA 
ACTGAAGTGA CGGGCTACTT CCCCAAGGGC ACGTGGTACA ACATGCAGAT GGTGTCAGTG 
GATTCCCTCG GTACTCTCCC TTCTCCATCA TCGGCTTCAT CCTTCAGATC TGCTGTCCAG 
AGCAAGGGGC AGTGGCTGAC ACTGGAAGCC CCACTGGATA CCATCAACGT GCACCTGAGG 
GAGGGGTACA TCATACCGCT GCAGGGTCCC AGCCTCACAA CCACGGAGTC CCGAAAGCAG 
CCCATGGCTC TGGCTGTGGC ATTAACAGCA AGCGGCGAGG CCGATGGGGA GCTGTTCTGG 
GACGACGGGG AGAGCCTCGC GGTTCTGGAG CGTGGGGCCT ACACACTGGT CACCTTCTCA 
GCCAAGAACA ATACCATTGT GAACAAGTTA GTGCGTGTGA CCAAGGAGGG AGCTGAGCTA 
CAACTGAGGG AGGTGACCGT CTTGGGAGTG GCCACAGCTC CTACCCAGGT CCTTTCCAAC 
GGCATCCCTG TCTCCAATTT CACCTACAGC CCTGACAACA AGAGCCTGGC CATCCCTGTC 
TCACTGCTGA TGGGAGAGCT GTTTCAAATC AGCTGGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rescue of Pompe disease in mice by AAV-mediated liver delivery of secretable acid ?-glucosidase.
Science translational medicine9(418)(2017 Nov)
Puzzo F,Colella P,Biferi MG,Bali D,Paulk NK,Vidal P,Collaud F,Simon-Sola M,Charles S,Hardet R,Leborgne C,Meliani A,Cohen-Tannoudji M,Astord S,Gjata B,Sellier P,van Wittenberghe L,Vignaud A,Boisgerault F,Barkats M,Laforet P,Kay MA,Koeberl DD,Ronzitti G,Mingozzi F

AAV-mediated transcription factor EB (TFEB) gene delivery ameliorates muscle pathology and function in the murine model of Pompe Disease.
Scientific reports7(1)15089(2017 Nov)
Gatto F,Rossi B,Tarallo A,Polishchuk E,Polishchuk R,Carrella A,Nusco E,Alvino FG,Iacobellis F,De Leonibus E,Auricchio A,Diez-Roux G,Ballabio A,Parenti G

Airway smooth muscle dysfunction in Pompe (
American journal of physiology. Lung cellular and molecular physiology312(6)L873-L881(2017 Jun)
Keeler AM,Liu D,Zieger M,Xiong L,Salemi J,Bellv? K,Byrne BJ,Fuller DD,ZhuGe R,ElMallah MK

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Transcriptome assessment of the Pompe (Gaa-/-) mouse spinal cord indicates widespread neuropathology.
Physiological genomics48(11)785-794(2016 Nov)
Turner SMF,Falk DJ,Byrne BJ,Fuller DD

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Gaa cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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