Dtx1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dtx1
Entrez Gene ID	14357
Full Name	deltex 1, E3 ubiquitin ligase
Synonyms	Fxit1,mKIAA4160
General protein information	Preferred Names deltex 1, E3 ubiquitin ligase Names E3 ubiquitin-protein ligase DTX1 FXI-T1 RING-type E3 ubiquitin transferase DTX1 deltex 1 homolog deltex1 fractionated x-irradiation induced transcript 1 mDTX1 protein deltex-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 F\|5 60.64 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011248180.1	XP_011246482.1	E3 ubiquitin-protein ligase DTX1 isoform X1
XM_011248180.2	XP_011246482.1	E3 ubiquitin-protein ligase DTX1 isoform X1
XM_011248181.2	XP_011246483.1	E3 ubiquitin-protein ligase DTX1 isoform X1
NM_008052.3	NP_032078.2	E3 ubiquitin-protein ligase DTX1
XM_011248181.3	XP_011246483.1	E3 ubiquitin-protein ligase DTX1 isoform X1
XM_030254131.1	XP_030109991.1	E3 ubiquitin-protein ligase DTX1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	DTX1	NP_004407.2
Rattus norvegicus (Norway rat)	Dtx1	XP_006249474.1
Gallus gallus (chicken)	DTX1	XP_001233994.2
Xenopus tropicalis (tropical clawed frog)	dtx1	XP_002934314.1
Bos taurus (cattle)	DTX1	NP_001192663.1
Pan troglodytes (chimpanzee)	DTX1	XP_003952356.1
Mus musculus (house mouse)	Dtx1	NP_032078.2

Gene Ontology
Bibliography

Related articles in PubMed

Laminar flow downregulates Notch activity to promote lymphatic sprouting.
Choi D, Park E, Jung E, Seong YJ, Yoo J, Lee E, Hong M, Lee S, Ishida H, Burford J, Peti-Peterdi J, Adams RH, Srikanth S, Gwack Y, Chen CS, Vogel HJ, Koh CJ, Wong AK, Hong YK
The Journal of clinical investigation127(4)1225-1240(2017 Apr)

Deltex1 antagonizes HIF-1? and sustains the stability of regulatory T cells in vivo.
Hsiao HW, Hsu TS, Liu WH, Hsieh WC, Chou TF, Wu YJ, Jiang ST, Lai MZ
Nature communications66353(2015 Feb)

Deltex1 promotes protein kinase C? degradation and sustains Casitas B-lineage lymphoma expression.
Hsu TS, Hsiao HW, Wu PJ, Liu WH, Lai MZ
Journal of immunology (Baltimore, Md. : 1950)193(4)1672-80(2014 Aug)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
Tarantino C, Paolella G, Cozzuto L, Minopoli G, Pastore L, Parisi S, Russo T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

exerts one level of control on regulatory T-cell stability by sustaining the expression of Foxp3 protein
Title: Deltex1 antagonizes HIF-1? and sustains the stability of regulatory T cells in vivo.

DTX1, similar to Cbl-b and Itch, participates in T cell anergy-induced PKC-theta degradation. there is a coupling of DTX1 to Cbl-b in T cell anergy.
Title: Deltex1 promotes protein kinase C? degradation and sustains Casitas B-lineage lymphoma expression.

Data show that Deltex1 ablation enabled Id1-expressing progenitors to differentiate to the DN3 (CD44(-) CD25(+)) stage, which was accompanied by enhanced Notch1 expression in T-cell progenitors.
Title: Id1 attenuates Notch signaling and impairs T-cell commitment by elevating Deltex1 expression.

deficiency of DTX1 augmented T cell activation, conferred resistance to anergy induction, enhanced autoantibody generation, and increased inflammation
Title: Deltex1 is a target of the transcription factor NFAT that promotes T cell anergy.

Deltex can act as a negative regulator of Notch signals in T cells but that endogenous levels of Deltex1 and Deltex2 are not important for regulating Notch signals during thymocyte development[Deltex1]
Title: T cells develop normally in the absence of both Deltex1 and Deltex2.

The following Dtx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dtx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01288	XM_030254131.1 Latest version!	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu67774	XM_011248180.2 Latest version!	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu67774	XM_011248181.3 Latest version!	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu01288	NM_008052.3 Latest version!	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OMu67774	XM_011248180.1	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu67774	XM_011248181.2	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dtx1 ( XM_030254131.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109991.1 Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu01288
Clone ID Related Accession (Same CDS sequence)	NM_008052.3 , XM_030254131.1
Accession Version	XM_030254131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase DTX1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGAT GGCAGCCTGC AGTGTCCAAC CTGCAAAGCC 
ATCTACGGGG AGAAGACAGG GACACAGCCA CCAGGGAAGA TGGAGTTTCA CCTCATCCCG 
CACTCGCTGC CTGGTTTTGC AGACACCCAG ACGATCCGCA TCGTCTATGA CATCCCCACG 
GGCATCCAGG GCCCTGAACA TCCCAACCCA GGCAAGAAGT TCACAGCCAG AGGCTTCCCT 
CGCCACTGCT ACCTACCCAA CAATGAGAAG GGCCGAAAGG TGCTGAGATT GCTCATCACC 
GCCTGGGAAC GCAGACTCAT CTTCACTATC GGAACATCCA ACACCACGGG CGAGTCGGAC 
ACCGTGGTGT GGAACGAGAT TCACCACAAG ACGGAGTTTG GTTCCAACCT CACTGGTCAC 
GGCTACCCCG ACGCCAGCTA CCTAGACAAC GTGCTGGCTG AGCTCACCGC CCAGGGGGTT 
TCTGAGGCCA TGGCCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109991.1
CDS	1188..3071
Translation	MSRPGQGVMVPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPIAPAAPPAPPPPPPPLPPGGPPGALVVRPSATFAGAALWAAPATGPTEPAPPPGVPPRSPSAPNGAPTPGQNNLSRPGPQRSTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTASGYEGVLRNKSVRPELVGRLGRCGHMYHLLCLVAMYSNGNKDGSLQCPTCKAIYGEKTGTQPPGKMEFHLIPHSLPGFADTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLIFTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAMAKA

Target ORF information:

RefSeq Version	XM_030254131.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254131.1

ORF Insert Sequence:

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGAT GGCAGCCTGC AGTGTCCAAC CTGCAAAGCC 
ATCTACGGGG AGAAGACAGG GACACAGCCA CCAGGGAAGA TGGAGTTTCA CCTCATCCCG 
CACTCGCTGC CTGGTTTTGC AGACACCCAG ACGATCCGCA TCGTCTATGA CATCCCCACG 
GGCATCCAGG GCCCTGAACA TCCCAACCCA GGCAAGAAGT TCACAGCCAG AGGCTTCCCT 
CGCCACTGCT ACCTACCCAA CAATGAGAAG GGCCGAAAGG TGCTGAGATT GCTCATCACC 
GCCTGGGAAC GCAGACTCAT CTTCACTATC GGAACATCCA ACACCACGGG CGAGTCGGAC 
ACCGTGGTGT GGAACGAGAT TCACCACAAG ACGGAGTTTG GTTCCAACCT CACTGGTCAC 
GGCTACCCCG ACGCCAGCTA CCTAGACAAC GTGCTGGCTG AGCTCACCGC CCAGGGGGTT 
TCTGAGGCCA TGGCCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dtx1 ( XM_011248180.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246482.1 Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67774
Clone ID Related Accession (Same CDS sequence)	XM_011248180.1 , XM_011248180.2 , XM_011248181.2 , XM_011248181.3
Accession Version	XM_011248180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase DTX1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGTG GGCCACAAGG ATGGCAGCCT GCAGTGTCCA 
ACCTGCAAAG CCATCTACGG GGAGAAGACA GGGACACAGC CACCAGGGAA GATGGAGTTT 
CACCTCATCC CGCACTCGCT GCCTGGTTTT GCAGACACCC AGACGATCCG CATCGTCTAT 
GACATCCCCA CGGGCATCCA GGGCCCTGAA CATCCCAACC CAGGCAAGAA GTTCACAGCC 
AGAGGCTTCC CTCGCCACTG CTACCTACCC AACAATGAGA AGGGCCGAAA GGTGCTGAGA 
TTGCTCATCA CCGCCTGGGA ACGCAGACTC ATCTTCACTA TCGGAACATC CAACACCACG 
GGCGAGTCGG ACACCGTGGT GTGGAACGAG ATTCACCACA AGACGGAGTT TGGTTCCAAC 
CTCACTGGTC ACGGCTACCC CGACGCCAGC TACCTAGACA ACGTGCTGGC TGAGCTCACC 
GCCCAGGGGG TTTCTGAGGC CATGGCCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246482.1
CDS	1046..2941
Translation	MSRPGQGVMVPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPIAPAAPPAPPPPPPPLPPGGPPGALVVRPSATFAGAALWAAPATGPTEPAPPPGVPPRSPSAPNGAPTPGQNNLSRPGPQRSTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTASGYEGVLRNKSVRPELVGRLGRCGHMYHLLCLVAMYSNGNKVGHKDGSLQCPTCKAIYGEKTGTQPPGKMEFHLIPHSLPGFADTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLIFTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAMAKA

Target ORF information:

RefSeq Version	XM_011248180.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248180.2

ORF Insert Sequence:

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGTG GGCCACAAGG ATGGCAGCCT GCAGTGTCCA 
ACCTGCAAAG CCATCTACGG GGAGAAGACA GGGACACAGC CACCAGGGAA GATGGAGTTT 
CACCTCATCC CGCACTCGCT GCCTGGTTTT GCAGACACCC AGACGATCCG CATCGTCTAT 
GACATCCCCA CGGGCATCCA GGGCCCTGAA CATCCCAACC CAGGCAAGAA GTTCACAGCC 
AGAGGCTTCC CTCGCCACTG CTACCTACCC AACAATGAGA AGGGCCGAAA GGTGCTGAGA 
TTGCTCATCA CCGCCTGGGA ACGCAGACTC ATCTTCACTA TCGGAACATC CAACACCACG 
GGCGAGTCGG ACACCGTGGT GTGGAACGAG ATTCACCACA AGACGGAGTT TGGTTCCAAC 
CTCACTGGTC ACGGCTACCC CGACGCCAGC TACCTAGACA ACGTGCTGGC TGAGCTCACC 
GCCCAGGGGG TTTCTGAGGC CATGGCCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dtx1 ( XM_011248181.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246483.1 Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X2, mRNA.

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CloneID	OMu67774
Clone ID Related Accession (Same CDS sequence)	XM_011248180.1 , XM_011248180.2 , XM_011248181.2 , XM_011248181.3
Accession Version	XM_011248181.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase DTX1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGTG GGCCACAAGG ATGGCAGCCT GCAGTGTCCA 
ACCTGCAAAG CCATCTACGG GGAGAAGACA GGGACACAGC CACCAGGGAA GATGGAGTTT 
CACCTCATCC CGCACTCGCT GCCTGGTTTT GCAGACACCC AGACGATCCG CATCGTCTAT 
GACATCCCCA CGGGCATCCA GGGCCCTGAA CATCCCAACC CAGGCAAGAA GTTCACAGCC 
AGAGGCTTCC CTCGCCACTG CTACCTACCC AACAATGAGA AGGGCCGAAA GGTGCTGAGA 
TTGCTCATCA CCGCCTGGGA ACGCAGACTC ATCTTCACTA TCGGAACATC CAACACCACG 
GGCGAGTCGG ACACCGTGGT GTGGAACGAG ATTCACCACA AGACGGAGTT TGGTTCCAAC 
CTCACTGGTC ACGGCTACCC CGACGCCAGC TACCTAGACA ACGTGCTGGC TGAGCTCACC 
GCCCAGGGGG TTTCTGAGGC CATGGCCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246483.1
CDS	774..2669
Translation	MSRPGQGVMVPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPIAPAAPPAPPPPPPPLPPGGPPGALVVRPSATFAGAALWAAPATGPTEPAPPPGVPPRSPSAPNGAPTPGQNNLSRPGPQRSTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTASGYEGVLRNKSVRPELVGRLGRCGHMYHLLCLVAMYSNGNKVGHKDGSLQCPTCKAIYGEKTGTQPPGKMEFHLIPHSLPGFADTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLIFTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAMAKA

Target ORF information:

RefSeq Version	XM_011248181.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248181.3

ORF Insert Sequence:

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGTG GGCCACAAGG ATGGCAGCCT GCAGTGTCCA 
ACCTGCAAAG CCATCTACGG GGAGAAGACA GGGACACAGC CACCAGGGAA GATGGAGTTT 
CACCTCATCC CGCACTCGCT GCCTGGTTTT GCAGACACCC AGACGATCCG CATCGTCTAT 
GACATCCCCA CGGGCATCCA GGGCCCTGAA CATCCCAACC CAGGCAAGAA GTTCACAGCC 
AGAGGCTTCC CTCGCCACTG CTACCTACCC AACAATGAGA AGGGCCGAAA GGTGCTGAGA 
TTGCTCATCA CCGCCTGGGA ACGCAGACTC ATCTTCACTA TCGGAACATC CAACACCACG 
GGCGAGTCGG ACACCGTGGT GTGGAACGAG ATTCACCACA AGACGGAGTT TGGTTCCAAC 
CTCACTGGTC ACGGCTACCC CGACGCCAGC TACCTAGACA ACGTGCTGGC TGAGCTCACC 
GCCCAGGGGG TTTCTGAGGC CATGGCCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dtx1 ( NM_008052.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032078.2 Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), mRNA.

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CloneID	OMu01288
Clone ID Related Accession (Same CDS sequence)	NM_008052.3 , XM_030254131.1
Accession Version	NM_008052.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase DTX1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK169455.1. On Apr 5, 2007 this sequence version replaced NM_008052.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK169455.1, AK088630.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGAT GGCAGCCTGC AGTGTCCAAC CTGCAAAGCC 
ATCTACGGGG AGAAGACAGG GACACAGCCA CCAGGGAAGA TGGAGTTTCA CCTCATCCCG 
CACTCGCTGC CTGGTTTTGC AGACACCCAG ACGATCCGCA TCGTCTATGA CATCCCCACG 
GGCATCCAGG GCCCTGAACA TCCCAACCCA GGCAAGAAGT TCACAGCCAG AGGCTTCCCT 
CGCCACTGCT ACCTACCCAA CAATGAGAAG GGCCGAAAGG TGCTGAGATT GCTCATCACC 
GCCTGGGAAC GCAGACTCAT CTTCACTATC GGAACATCCA ACACCACGGG CGAGTCGGAC 
ACCGTGGTGT GGAACGAGAT TCACCACAAG ACGGAGTTTG GTTCCAACCT CACTGGTCAC 
GGCTACCCCG ACGCCAGCTA CCTAGACAAC GTGCTGGCTG AGCTCACCGC CCAGGGGGTT 
TCTGAGGCCA TGGCCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032078.2
CDS	848..2731
Translation	MSRPGQGVMVPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPIAPAAPPAPPPPPPPLPPGGPPGALVVRPSATFAGAALWAAPATGPTEPAPPPGVPPRSPSAPNGAPTPGQNNLSRPGPQRSTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTASGYEGVLRNKSVRPELVGRLGRCGHMYHLLCLVAMYSNGNKDGSLQCPTCKAIYGEKTGTQPPGKMEFHLIPHSLPGFADTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLIFTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAMAKA

Target ORF information:

RefSeq Version	NM_008052.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008052.3

ORF Insert Sequence:

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGAT GGCAGCCTGC AGTGTCCAAC CTGCAAAGCC 
ATCTACGGGG AGAAGACAGG GACACAGCCA CCAGGGAAGA TGGAGTTTCA CCTCATCCCG 
CACTCGCTGC CTGGTTTTGC AGACACCCAG ACGATCCGCA TCGTCTATGA CATCCCCACG 
GGCATCCAGG GCCCTGAACA TCCCAACCCA GGCAAGAAGT TCACAGCCAG AGGCTTCCCT 
CGCCACTGCT ACCTACCCAA CAATGAGAAG GGCCGAAAGG TGCTGAGATT GCTCATCACC 
GCCTGGGAAC GCAGACTCAT CTTCACTATC GGAACATCCA ACACCACGGG CGAGTCGGAC 
ACCGTGGTGT GGAACGAGAT TCACCACAAG ACGGAGTTTG GTTCCAACCT CACTGGTCAC 
GGCTACCCCG ACGCCAGCTA CCTAGACAAC GTGCTGGCTG AGCTCACCGC CCAGGGGGTT 
TCTGAGGCCA TGGCCAAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dtx1 ( XM_011248180.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246482.1 Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X1, mRNA.

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CloneID	OMu67774
Clone ID Related Accession (Same CDS sequence)	XM_011248180.1 , XM_011248180.2 , XM_011248181.2 , XM_011248181.3
Accession Version	XM_011248180.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase DTX1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGTG GGCCACAAGG ATGGCAGCCT GCAGTGTCCA 
ACCTGCAAAG CCATCTACGG GGAGAAGACA GGGACACAGC CACCAGGGAA GATGGAGTTT 
CACCTCATCC CGCACTCGCT GCCTGGTTTT GCAGACACCC AGACGATCCG CATCGTCTAT 
GACATCCCCA CGGGCATCCA GGGCCCTGAA CATCCCAACC CAGGCAAGAA GTTCACAGCC 
AGAGGCTTCC CTCGCCACTG CTACCTACCC AACAATGAGA AGGGCCGAAA GGTGCTGAGA 
TTGCTCATCA CCGCCTGGGA ACGCAGACTC ATCTTCACTA TCGGAACATC CAACACCACG 
GGCGAGTCGG ACACCGTGGT GTGGAACGAG ATTCACCACA AGACGGAGTT TGGTTCCAAC 
CTCACTGGTC ACGGCTACCC CGACGCCAGC TACCTAGACA ACGTGCTGGC TGAGCTCACC 
GCCCAGGGGG TTTCTGAGGC CATGGCCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246482.1
CDS	842..2737
Translation	MSRPGQGVMVPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPIAPAAPPAPPPPPPPLPPGGPPGALVVRPSATFAGAALWAAPATGPTEPAPPPGVPPRSPSAPNGAPTPGQNNLSRPGPQRSTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTASGYEGVLRNKSVRPELVGRLGRCGHMYHLLCLVAMYSNGNKVGHKDGSLQCPTCKAIYGEKTGTQPPGKMEFHLIPHSLPGFADTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLIFTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAMAKA

Target ORF information:

RefSeq Version	XM_011248180.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248180.1

ORF Insert Sequence:

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGTG GGCCACAAGG ATGGCAGCCT GCAGTGTCCA 
ACCTGCAAAG CCATCTACGG GGAGAAGACA GGGACACAGC CACCAGGGAA GATGGAGTTT 
CACCTCATCC CGCACTCGCT GCCTGGTTTT GCAGACACCC AGACGATCCG CATCGTCTAT 
GACATCCCCA CGGGCATCCA GGGCCCTGAA CATCCCAACC CAGGCAAGAA GTTCACAGCC 
AGAGGCTTCC CTCGCCACTG CTACCTACCC AACAATGAGA AGGGCCGAAA GGTGCTGAGA 
TTGCTCATCA CCGCCTGGGA ACGCAGACTC ATCTTCACTA TCGGAACATC CAACACCACG 
GGCGAGTCGG ACACCGTGGT GTGGAACGAG ATTCACCACA AGACGGAGTT TGGTTCCAAC 
CTCACTGGTC ACGGCTACCC CGACGCCAGC TACCTAGACA ACGTGCTGGC TGAGCTCACC 
GCCCAGGGGG TTTCTGAGGC CATGGCCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dtx1 ( XM_011248181.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246483.1 Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X2, mRNA.

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CloneID	OMu67774
Clone ID Related Accession (Same CDS sequence)	XM_011248180.1 , XM_011248180.2 , XM_011248181.2 , XM_011248181.3
Accession Version	XM_011248181.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase DTX1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGTG GGCCACAAGG ATGGCAGCCT GCAGTGTCCA 
ACCTGCAAAG CCATCTACGG GGAGAAGACA GGGACACAGC CACCAGGGAA GATGGAGTTT 
CACCTCATCC CGCACTCGCT GCCTGGTTTT GCAGACACCC AGACGATCCG CATCGTCTAT 
GACATCCCCA CGGGCATCCA GGGCCCTGAA CATCCCAACC CAGGCAAGAA GTTCACAGCC 
AGAGGCTTCC CTCGCCACTG CTACCTACCC AACAATGAGA AGGGCCGAAA GGTGCTGAGA 
TTGCTCATCA CCGCCTGGGA ACGCAGACTC ATCTTCACTA TCGGAACATC CAACACCACG 
GGCGAGTCGG ACACCGTGGT GTGGAACGAG ATTCACCACA AGACGGAGTT TGGTTCCAAC 
CTCACTGGTC ACGGCTACCC CGACGCCAGC TACCTAGACA ACGTGCTGGC TGAGCTCACC 
GCCCAGGGGG TTTCTGAGGC CATGGCCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246483.1
CDS	917..2812
Translation	MSRPGQGVMVPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPIAPAAPPAPPPPPPPLPPGGPPGALVVRPSATFAGAALWAAPATGPTEPAPPPGVPPRSPSAPNGAPTPGQNNLSRPGPQRSTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTASGYEGVLRNKSVRPELVGRLGRCGHMYHLLCLVAMYSNGNKVGHKDGSLQCPTCKAIYGEKTGTQPPGKMEFHLIPHSLPGFADTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLIFTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAMAKA

Target ORF information:

RefSeq Version	XM_011248181.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus deltex 1, E3 ubiquitin ligase (Dtx1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248181.2

ORF Insert Sequence:

ATGTCACGGC CAGGCCAAGG TGTGATGGTG CCGGTGAACG GGCTGGGCTT CCCGCCGCAG 
AACGTGGCCA GGGTGGTGGT GTGGGAGTGG CTCAATGAGC ACAGCCGCTG GCGACCCTAC 
ACGGCCACCG TGTGCCACCA CATCGAGAAT GTTCTTAAGG AGGATGCCCG GGGCTCTGTG 
GTCCTTGGCC AGGTGGACGC CCAGCTGGTG CCCTACATCA TCGACCTACA ATCCATGCAT 
CAGTTCCGGC AAGACACAGG TACCATGCGG CCGGTGCGAC GCAACTTCTA CGATCCATCG 
TCGGCGCCGG GCAAGGGCAT CGTGTGGGAA TGGGAGAACG ACGGCGGGGC GTGGACGGCC 
TACGACATGG ACATCTGCAT CACCATCCAG AACGCGTACG AGAAGCAGCA CCCGTGGCTC 
GACCTCTCAT CGCTCGGCTT CTGCTACCTC ATCTACTTCA ACAGCATGTC CCAGATGAAC 
CGCCAGACGC GCCGCCGCCG CCGCCTGCGC CGTCGCCTGG ACCTGGCTTA CCCGCTCACT 
GTCGGCTCCA TTCCCAAGTC GCAATCCTGG CCCGTGGGAG CCAGCTCGGG TCAGCCCTGC 
TCCTGTCAGC AGTGCCTGCT GGTCAACAGC ACGCGCGCCG CCTCCAACGC CATCCTGGCC 
TCGCAGCGCC GCAAGGCTCC CATTGCGCCA GCCGCGCCTC CAGCGCCCCC TCCGCCCCCG 
CCGCCGCTGC CACCCGGAGG ACCTCCGGGT GCGCTCGTTG TGCGCCCCAG CGCCACTTTC 
GCCGGAGCTG CGCTCTGGGC CGCACCTGCC ACCGGCCCCA CGGAGCCTGC GCCGCCTCCA 
GGAGTTCCCC CAAGGAGCCC TAGTGCCCCC AACGGAGCGC CCACACCGGG CCAAAACAAC 
CTCAGTCGAC CAGGACCACA GAGGTCCACC AGCGTCAGCG CACGCGCCTC TATCCCGCCT 
GGGGTTCCGG CGCTCCCCGT GAAGAACTTG AATGGCACTG GCCCTGTCCA CCCAGCCTTG 
GCAGGGATGA CCGGGATCCT GCTGTGTGCA GCGGGGCTGC CGGTGTGCCT GACACGAGCA 
CCCAAACCCA TCCTGCACCC ACCACCAGTA AGCAAAAGCG ACGTGAAGCC TGTGCCTGGA 
GTGCCCGGCG TGTGCCGCAA GACCAAGAAG AAACACCTCA AGAAAAGCAA GAATCCTGAG 
GATGTGGTTC GGAGGTACAT GCAGAAGGTG AAAAACCCGC CTGATGAGGA CTGTACCATT 
TGCATGGAGC GGCTGGTCAC AGCATCTGGC TATGAGGGCG TGCTCCGAAA CAAGAGTGTG 
CGGCCCGAGC TTGTGGGCCG CCTGGGCCGC TGCGGCCACA TGTATCACCT GCTCTGCCTG 
GTGGCCATGT ACTCCAATGG CAACAAGGTG GGCCACAAGG ATGGCAGCCT GCAGTGTCCA 
ACCTGCAAAG CCATCTACGG GGAGAAGACA GGGACACAGC CACCAGGGAA GATGGAGTTT 
CACCTCATCC CGCACTCGCT GCCTGGTTTT GCAGACACCC AGACGATCCG CATCGTCTAT 
GACATCCCCA CGGGCATCCA GGGCCCTGAA CATCCCAACC CAGGCAAGAA GTTCACAGCC 
AGAGGCTTCC CTCGCCACTG CTACCTACCC AACAATGAGA AGGGCCGAAA GGTGCTGAGA 
TTGCTCATCA CCGCCTGGGA ACGCAGACTC ATCTTCACTA TCGGAACATC CAACACCACG 
GGCGAGTCGG ACACCGTGGT GTGGAACGAG ATTCACCACA AGACGGAGTT TGGTTCCAAC 
CTCACTGGTC ACGGCTACCC CGACGCCAGC TACCTAGACA ACGTGCTGGC TGAGCTCACC 
GCCCAGGGGG TTTCTGAGGC CATGGCCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Laminar flow downregulates Notch activity to promote lymphatic sprouting.
The Journal of clinical investigation127(4)1225-1240(2017 Apr)
Choi D,Park E,Jung E,Seong YJ,Yoo J,Lee E,Hong M,Lee S,Ishida H,Burford J,Peti-Peterdi J,Adams RH,Srikanth S,Gwack Y,Chen CS,Vogel HJ,Koh CJ,Wong AK,Hong YK

Deltex1 antagonizes HIF-1? and sustains the stability of regulatory T cells in vivo.
Nature communications66353(2015 Feb)
Hsiao HW,Hsu TS,Liu WH,Hsieh WC,Chou TF,Wu YJ,Jiang ST,Lai MZ

Deltex1 promotes protein kinase C? degradation and sustains Casitas B-lineage lymphoma expression.
Journal of immunology (Baltimore, Md. : 1950)193(4)1672-80(2014 Aug)
Hsu TS,Hsiao HW,Wu PJ,Liu WH,Lai MZ

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)
Tarantino C,Paolella G,Cozzuto L,Minopoli G,Pastore L,Parisi S,Russo T

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Dtx1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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