Ncs1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncs1
Entrez Gene ID	14299
Full Name	neuronal calcium sensor 1
Synonyms	9430075O15Rik,A730032G13Rik,AI836659,Freq,Mfreq,NCS-1
General protein information	Preferred Names neuronal calcium sensor 1 Names neuronal calcium sensor 1 frequenin homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 B

mRNA and Protein(s)

mRNA	Protein	Name
NM_019681.3	NP_062655.1	neuronal calcium sensor 1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013126	XP_003437186.1
Neurospora crassa	NCU04379	XP_957456.1
Mus musculus (house mouse)	Ncs1	NP_062655.1
Gallus gallus (chicken)	NCS1	NP_990708.1
Drosophila melanogaster (fruit fly)	Frq1	NP_573271.1
Kluyveromyces lactis	KLLA0E02399g	XP_454056.1
Schizosaccharomyces pombe (fission yeast)	ncs1	NP_592879.1
Danio rerio (zebrafish)	ncs1b	NP_001018350.1
Eremothecium gossypii	AGOS_ACL163W	NP_983241.1
Homo sapiens (human)	NCS1	NP_055101.2
Bos taurus (cattle)	NCS1	XP_005213538.1
Rattus norvegicus (Norway rat)	Ncs1	NP_077342.1
Xenopus tropicalis (tropical clawed frog)	ncs1	NP_988973.1
Canis lupus familiaris (dog)	NCS1	XP_005625293.1
Drosophila melanogaster (fruit fly)	Frq2	NP_001259675.1
Caenorhabditis elegans (roundworm)	ncs-1	NP_508186.1
Saccharomyces cerevisiae (baker's yeast)	FRQ1	NP_010661.3
Magnaporthe oryzae (rice blast fungus)	MGG_01550	XP_003714524.1

Gene Ontology
Bibliography

Related articles in PubMed

Possible Signaling Pathways Mediating Neuronal Calcium Sensor-1-Dependent Spatial Learning and Memory in Mice.
Nakamura TY, Nakao S, Nakajo Y, Takahashi JC, Wakabayashi S, Yanamoto H
PloS one12(1)e0170829(2017)

Neuronal Ca
Nakamura TY, Nakao S, Wakabayashi S
Journal of molecular and cellular cardiology9923-34(2016 Oct)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Neuronal calcium sensor-1 deletion in the mouse decreases motivation and dopamine release in the nucleus accumbens.
Ng E, Varaschin RK, Su P, Browne CJ, Hermainski J, Le Foll B, Pongs O, Liu F, Trudeau LE, Roder JC, Wong AH
Behavioural brain research301213-25(2016 Mar)

Self-directed exploration provides a Ncs1-dependent learning bonus.
Mun HS, Saab BJ, Ng E, McGirr A, Lipina TV, Gondo Y, Georgiou J, Roder JC
Scientific reports517697(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phosphorylation of CaMKII-alpha, known to trigger long-term potentiation and increase BDNF levels, was significantly reduced in the Ncs1-/- mouse brain. Further, electric potential stimulation, which increases the levels of BDNF and promotes spatial learning, significantly increased the levels of NCS-1 concomitant with phosphorylated CaMKII-alpha in the hippocampus; suggesting a close relationship between NCS-1 and CaM...
Title: Possible Signaling Pathways Mediating Neuronal Calcium Sensor-1-Dependent Spatial Learning and Memory in Mice.

these data demonstrate that NCS-1 contributes to stress tolerance in cardiomyocytes at least in part by activating certain Ca(2+)-dependent survival pathways that promote mitochondrial biosynthesis/function and detoxification pathways.
Title: Neuronal Ca

These studies identify novel roles for NCS-1 in regulating activity-dependent striatal dopamine release and aspects of motivated behavior
Title: Neuronal calcium sensor-1 deletion in the mouse decreases motivation and dopamine release in the nucleus accumbens.

elevated expression of neuronal calcium sensor 1 (Ncs1) and dopamine type-2 receptors upon self-directed exploration, is reported.
Title: Self-directed exploration provides a Ncs1-dependent learning bonus.

IGF-1 strengthens the interaction between NCS-1 and IP3R in the process of regulation of nuclear Ca2+ signaling in cardiomyocytes.
Title: Stimulus-dependent regulation of nuclear Ca2+ signaling in cardiomyocytes: a role of neuronal calcium sensor-1.

The following Ncs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11602	NM_019681.3 Latest version!	Mus musculus neuronal calcium sensor 1 (Ncs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncs1 ( NM_019681.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062655.1 Mus musculus neuronal calcium sensor 1 (Ncs1), mRNA.

Price & Availability↑

CloneID	OMu11602
Clone ID Related Accession (Same CDS sequence)	NM_019681.3
Accession Version	NM_019681.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	neuronal calcium sensor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL732572.10. On Nov 17, 2006 this sequence version replaced NM_019681.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC059825.1, SRR1660821.290937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAAAT CCAACAGCAA GTTGAAGCCT GAAGTTGTGG AGGAGCTGAC CAGAAAAACC 
TACTTCACTG AGAAAGAAGT ACAGCAGTGG TACAAGGGCT TCATTAAGGA CTGCCCCAGC 
GGGCAGCTGG ATGCCGCTGG CTTCCAGAAG ATCTACAAGC AGTTCTTCCC ATTTGGAGAC 
CCCACCAAGT TCGCCACGTT TGTTTTCAAC GTCTTCGACG AGAACAAGGA TGGCAGGATT 
GAGTTCTCCG AGTTCATCCA GGCACTGTCG GTGACCTCAA GGGGGACCCT GGATGAAAAG 
CTGCGATGGG CCTTCAAGCT TTATGACTTG GATAACGATG GCTACATCAC CAGGAACGAG 
ATGCTCGACA TAGTGGACGC CATTTACCAG ATGGTGGGGA ACACCGTGGA GCTCCCAGAA 
GAAGAGAACA CACCAGAGAA GAGGGTGGAC CGGATCTTTG CCATGATGGA CAAGAATGCT 
GATGGGAAGC TGACTCTTCA GGAGTTCCAG GAAGGCTCCA AGGCTGATCC CTCCATTGTG 
CAGGCGCTGT CCCTCTACGA TGGGCTGGTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062655.1
CDS	257..829
Translation	MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV

Target ORF information:

RefSeq Version	NM_019681.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuronal calcium sensor 1 (Ncs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019681.3

ORF Insert Sequence:

ATGGGGAAAT CCAACAGCAA GTTGAAGCCT GAAGTTGTGG AGGAGCTGAC CAGAAAAACC 
TACTTCACTG AGAAAGAAGT ACAGCAGTGG TACAAGGGCT TCATTAAGGA CTGCCCCAGC 
GGGCAGCTGG ATGCCGCTGG CTTCCAGAAG ATCTACAAGC AGTTCTTCCC ATTTGGAGAC 
CCCACCAAGT TCGCCACGTT TGTTTTCAAC GTCTTCGACG AGAACAAGGA TGGCAGGATT 
GAGTTCTCCG AGTTCATCCA GGCACTGTCG GTGACCTCAA GGGGGACCCT GGATGAAAAG 
CTGCGATGGG CCTTCAAGCT TTATGACTTG GATAACGATG GCTACATCAC CAGGAACGAG 
ATGCTCGACA TAGTGGACGC CATTTACCAG ATGGTGGGGA ACACCGTGGA GCTCCCAGAA 
GAAGAGAACA CACCAGAGAA GAGGGTGGAC CGGATCTTTG CCATGATGGA CAAGAATGCT 
GATGGGAAGC TGACTCTTCA GGAGTTCCAG GAAGGCTCCA AGGCTGATCC CTCCATTGTG 
CAGGCGCTGT CCCTCTACGA TGGGCTGGTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Possible Signaling Pathways Mediating Neuronal Calcium Sensor-1-Dependent Spatial Learning and Memory in Mice.
PloS one12(1)e0170829(2017)
Nakamura TY,Nakao S,Nakajo Y,Takahashi JC,Wakabayashi S,Yanamoto H

Neuronal Ca
Journal of molecular and cellular cardiology9923-34(2016 Oct)
Nakamura TY,Nakao S,Wakabayashi S

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Neuronal calcium sensor-1 deletion in the mouse decreases motivation and dopamine release in the nucleus accumbens.
Behavioural brain research301213-25(2016 Mar)
Ng E,Varaschin RK,Su P,Browne CJ,Hermainski J,Le Foll B,Pongs O,Liu F,Trudeau LE,Roder JC,Wong AH

Self-directed exploration provides a Ncs1-dependent learning bonus.
Scientific reports517697(2015 Dec)
Mun HS,Saab BJ,Ng E,McGirr A,Lipina TV,Gondo Y,Georgiou J,Roder JC

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Ncs1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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