Fxn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fxn
Entrez Gene ID	14297
Full Name	frataxin
Synonyms	FA,FARR,Frda,X25
General protein information	Preferred Names frataxin Names frataxin, mitochondrial Friedreich ataxia
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 B

mRNA and Protein(s)

mRNA	Protein	Name
NM_008044.3	NP_032070.1	frataxin, mitochondrial precursor
NM_008044.2	NP_032070.1	frataxin, mitochondrial precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FXN	NP_001074196.1
Gallus gallus (chicken)	FXN	XP_424827.3
Homo sapiens (human)	FXN	NP_000135.2
Pan troglodytes (chimpanzee)	FXN	XP_001137864.2
Danio rerio (zebrafish)	fxn	NP_001076485.1
Arabidopsis thaliana (thale cress)	FH	NP_192233.2
Xenopus tropicalis (tropical clawed frog)	LOC100497789	XP_004910856.1
	FXN	XP_001093449.1
Mus musculus (house mouse)	Fxn	NP_032070.1
Rattus norvegicus (Norway rat)	Fxn	NP_001178881.1
Canis lupus familiaris (dog)	FXN	NP_001103428.1
Oryza sativa (rice)	Os01g0783600	NP_001044455.2

Gene Ontology
Bibliography

Related articles in PubMed

Early VGLUT1-specific parallel fiber synaptic deficits and dysregulated cerebellar circuit in the KIKO mouse model of Friedreich ataxia.
Lin H, Magrane J, Clark EM, Halawani SM, Warren N, Rattelle A, Lynch DR
Disease models & mechanisms10(12)1529-1538(2017 Dec)

Early cerebellar deficits in mitochondrial biogenesis and respiratory chain complexes in the KIKO mouse model of Friedreich ataxia.
Lin H, Magrane J, Rattelle A, Stepanova A, Galkin A, Clark EM, Dong YN, Halawani SM, Lynch DR
Disease models & mechanisms10(11)1343-1352(2017 Nov)

Transplantation of wild-type mouse hematopoietic stem and progenitor cells ameliorates deficits in a mouse model of Friedreich's ataxia.
Rocca CJ, Goodman SM, Dulin JN, Haquang JH, Gertsman I, Blondelle J, Smith JLM, Heyser CJ, Cherqui S
Science translational medicine9(413)(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that mouse model of Friedreich's ataxia showed that decreased grip strength endurance time , threshold of peripheral sensitivity using Von Frey monofilaments and gait parameters.
Title: Neurobehavioral deficits in the KIKO mouse model of Friedreich's ataxia.

Here, the authors show that loss of Fxn in the nervous system in mice also activates an iron/sphingolipid/PDK1/Mef2 pathway, indicating that the mechanism is evolutionarily conserved.
Title: Loss of Frataxin activates the iron/sphingolipid/PDK1/Mef2 pathway in mammals.

The in vitro antioxidant treatments trigger the axonal re-growth and the increase in stable MTs in shFxn, thus contributing to identify new neuronal targets of oxidation in this disease and providing a novel approach for antioxidant therapies.
Title: Frataxin silencing alters microtubule stability in motor neurons: implications for Friedreich's ataxia.

Retinal FXN levels are increased in response to ischemia. Furthermore, elevated FXN levels had a clear neuroprotective effect as shown by increased ganglion cell survival after acute retinal ischemia/reperfusion. Frataxin's neuroprotective effect was associated with an upregulation of antioxidative enzymes.
Title: Increased Frataxin Levels Protect Retinal Ganglion Cells After Acute Ischemia/Reperfusion in the Mouse Retina In Vivo.

Frataxin Deficiency Promotes Excess Microglial DNA Damage and Inflammation that Is Rescued by PJ34
Title: Frataxin Deficiency Promotes Excess Microglial DNA Damage and Inflammation that Is Rescued by PJ34.

The following Fxn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fxn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14153	NM_008044.3 Latest version!	Mus musculus frataxin (Fxn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu14153	NM_008044.2	Mus musculus frataxin (Fxn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fxn ( NM_008044.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032070.1 Mus musculus frataxin (Fxn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14153
Clone ID Related Accession (Same CDS sequence)	NM_008044.3 , NM_008044.2
Accession Version	NM_008044.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	frataxin, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC165089.5 and AC149224.7. On Jul 21, 2018 this sequence version replaced NM_008044.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.4054293.1, SRR7345562.948848.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCGT TCGGAGGTCG CGCAGCCGTG GGCTTGCTGC CCCGGACGGC GTCCCGGGCC 
TCCGCCTGGG TCGGGAACCC GCGCTGGAGG GAACCGATCG TAACCTGCGG CCGCCGAGGC 
CTACATGTCA CAGTCAACGC CGGCGCCACC CGCCACGCCC ATTTGAACCT CCACTACCTC 
CAGATTCTGA ACATCAAAAA GCAGAGCGTC TGCGTGGTGC ATTTGAGGAA CTTGGGGACA 
TTGGACAACC CAAGCTCTCT AGACGAGACA GCGTATGAAA GACTGGCGGA AGAGACCCTG 
GACTCCCTGG CCGAGTTCTT TGAAGACCTC GCAGACAAGC CCTATACCCT GGAGGACTAC 
GATGTCTCTT TTGGGGATGG CGTGCTCACC ATTAAGCTGG GCGGGGATCT AGGGACCTAC 
GTGATCAACA AGCAGACCCC AAACAAGCAA ATCTGGCTGT CTTCTCCTTC CAGCGGCCCC 
AAGCGCTATG ACTGGACCGG GAAGAACTGG GTGTACTCTC ATGACGGCGT GTCTCTGCAT 
GAGCTGCTGG CCAGGGAGCT GACTAAAGCT TTAAACACCA AACTGGACTT GTCTTCATTG 
GCCTATTCTG GAAAAGGCAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032070.1
CDS	26..649
Translation	MWAFGGRAAVGLLPRTASRASAWVGNPRWREPIVTCGRRGLHVTVNAGATRHAHLNLHYLQILNIKKQSVCVVHLRNLGTLDNPSSLDETAYERLAEETLDSLAEFFEDLADKPYTLEDYDVSFGDGVLTIKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWTGKNWVYSHDGVSLHELLARELTKALNTKLDLSSLAYSGKGT

Target ORF information:

RefSeq Version	NM_008044.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus frataxin (Fxn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008044.3

ORF Insert Sequence:

ATGTGGGCGT TCGGAGGTCG CGCAGCCGTG GGCTTGCTGC CCCGGACGGC GTCCCGGGCC 
TCCGCCTGGG TCGGGAACCC GCGCTGGAGG GAACCGATCG TAACCTGCGG CCGCCGAGGC 
CTACATGTCA CAGTCAACGC CGGCGCCACC CGCCACGCCC ATTTGAACCT CCACTACCTC 
CAGATTCTGA ACATCAAAAA GCAGAGCGTC TGCGTGGTGC ATTTGAGGAA CTTGGGGACA 
TTGGACAACC CAAGCTCTCT AGACGAGACA GCGTATGAAA GACTGGCGGA AGAGACCCTG 
GACTCCCTGG CCGAGTTCTT TGAAGACCTC GCAGACAAGC CCTATACCCT GGAGGACTAC 
GATGTCTCTT TTGGGGATGG CGTGCTCACC ATTAAGCTGG GCGGGGATCT AGGGACCTAC 
GTGATCAACA AGCAGACCCC AAACAAGCAA ATCTGGCTGT CTTCTCCTTC CAGCGGCCCC 
AAGCGCTATG ACTGGACCGG GAAGAACTGG GTGTACTCTC ATGACGGCGT GTCTCTGCAT 
GAGCTGCTGG CCAGGGAGCT GACTAAAGCT TTAAACACCA AACTGGACTT GTCTTCATTG 
GCCTATTCTG GAAAAGGCAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxn ( NM_008044.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032070.1 Mus musculus frataxin (Fxn), mRNA.

Price & Availability↑

CloneID	OMu14153
Clone ID Related Accession (Same CDS sequence)	NM_008044.3 , NM_008044.2
Accession Version	NM_008044.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Mus musculus(house mouse)
Product	frataxin, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC165089.5 and AC149224.7. On Mar 21, 2007 this sequence version replaced NM_008044.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U95736.1, BC125337.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTGGGCGT TCGGAGGTCG CGCAGCCGTG GGCTTGCTGC CCCGGACGGC GTCCCGGGCC 
TCCGCCTGGG TCGGGAACCC GCGCTGGAGG GAACCGATCG TAACCTGCGG CCGCCGAGGC 
CTACATGTCA CAGTCAACGC CGGCGCCACC CGCCACGCCC ATTTGAACCT CCACTACCTC 
CAGATTCTGA ACATCAAAAA GCAGAGCGTC TGCGTGGTGC ATTTGAGGAA CTTGGGGACA 
TTGGACAACC CAAGCTCTCT AGACGAGACA GCGTATGAAA GACTGGCGGA AGAGACCCTG 
GACTCCCTGG CCGAGTTCTT TGAAGACCTC GCAGACAAGC CCTATACCCT GGAGGACTAC 
GATGTCTCTT TTGGGGATGG CGTGCTCACC ATTAAGCTGG GCGGGGATCT AGGGACCTAC 
GTGATCAACA AGCAGACCCC AAACAAGCAA ATCTGGCTGT CTTCTCCTTC CAGCGGCCCC 
AAGCGCTATG ACTGGACCGG GAAGAACTGG GTGTACTCTC ATGACGGCGT GTCTCTGCAT 
GAGCTGCTGG CCAGGGAGCT GACTAAAGCT TTAAACACCA AACTGGACTT GTCTTCATTG 
GCCTATTCTG GAAAAGGCAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032070.1
CDS	22..645
Translation	MWAFGGRAAVGLLPRTASRASAWVGNPRWREPIVTCGRRGLHVTVNAGATRHAHLNLHYLQILNIKKQSVCVVHLRNLGTLDNPSSLDETAYERLAEETLDSLAEFFEDLADKPYTLEDYDVSFGDGVLTIKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWTGKNWVYSHDGVSLHELLARELTKALNTKLDLSSLAYSGKGT

Target ORF information:

RefSeq Version	NM_008044.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus frataxin (Fxn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008044.2

ORF Insert Sequence:

ATGTGGGCGT TCGGAGGTCG CGCAGCCGTG GGCTTGCTGC CCCGGACGGC GTCCCGGGCC 
TCCGCCTGGG TCGGGAACCC GCGCTGGAGG GAACCGATCG TAACCTGCGG CCGCCGAGGC 
CTACATGTCA CAGTCAACGC CGGCGCCACC CGCCACGCCC ATTTGAACCT CCACTACCTC 
CAGATTCTGA ACATCAAAAA GCAGAGCGTC TGCGTGGTGC ATTTGAGGAA CTTGGGGACA 
TTGGACAACC CAAGCTCTCT AGACGAGACA GCGTATGAAA GACTGGCGGA AGAGACCCTG 
GACTCCCTGG CCGAGTTCTT TGAAGACCTC GCAGACAAGC CCTATACCCT GGAGGACTAC 
GATGTCTCTT TTGGGGATGG CGTGCTCACC ATTAAGCTGG GCGGGGATCT AGGGACCTAC 
GTGATCAACA AGCAGACCCC AAACAAGCAA ATCTGGCTGT CTTCTCCTTC CAGCGGCCCC 
AAGCGCTATG ACTGGACCGG GAAGAACTGG GTGTACTCTC ATGACGGCGT GTCTCTGCAT 
GAGCTGCTGG CCAGGGAGCT GACTAAAGCT TTAAACACCA AACTGGACTT GTCTTCATTG 
GCCTATTCTG GAAAAGGCAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Early VGLUT1-specific parallel fiber synaptic deficits and dysregulated cerebellar circuit in the KIKO mouse model of Friedreich ataxia.
Disease models & mechanisms10(12)1529-1538(2017 Dec)
Lin H,Magrane J,Clark EM,Halawani SM,Warren N,Rattelle A,Lynch DR

Early cerebellar deficits in mitochondrial biogenesis and respiratory chain complexes in the KIKO mouse model of Friedreich ataxia.
Disease models & mechanisms10(11)1343-1352(2017 Nov)
Lin H,Magrane J,Rattelle A,Stepanova A,Galkin A,Clark EM,Dong YN,Halawani SM,Lynch DR

Transplantation of wild-type mouse hematopoietic stem and progenitor cells ameliorates deficits in a mouse model of Friedreich's ataxia.
Science translational medicine9(413)(2017 Oct)
Rocca CJ,Goodman SM,Dulin JN,Haquang JH,Gertsman I,Blondelle J,Smith JLM,Heyser CJ,Cherqui S

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Fxn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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