Flii cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Flii
Entrez Gene ID	14248
Full Name	flightless I actin binding protein
Synonyms	3632430F08Rik,Fliih
General protein information	Preferred Names flightless I actin binding protein Names protein flightless-1 homolog cytoskeletal actin-modulating protein flightless I homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B2\|11 37.81 cM
Summary	This gene encodes a protein with gelsolin-like repeats and an N-terminal leucine-rich repeat domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. This protein may act as an actin-remodelling protein as well as a transcriptional coactivator. Homozygous knockout mice show embryonic lethality. This protein may act to regulate wound repair. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001302207.1	NP_001289136.1	protein flightless-1 homolog isoform 2
NM_022009.2	NP_071292.1	protein flightless-1 homolog isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FLII	XP_511321.3
Bos taurus (cattle)	FLII	XP_002695913.2
Rattus norvegicus (Norway rat)	Flii	NP_001008280.1
Drosophila melanogaster (fruit fly)	fliI	NP_525097.1
Homo sapiens (human)	FLII	NP_002009.1
Mus musculus (house mouse)	Flii	NP_071292.1
Danio rerio (zebrafish)	flii	NP_001244075.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011292	XP_309356.4
Caenorhabditis elegans (roundworm)	fli-1	NP_498913.2
Xenopus tropicalis (tropical clawed frog)	flii	NP_001015848.1
Canis lupus familiaris (dog)	FLII	XP_005620209.1
	FLII	XP_001094628.2
Gallus gallus (chicken)	FLII	NP_001025806.1

Gene Ontology
Bibliography

Related articles in PubMed

TRPV4 mediates the Ca
Arora PD, Di Gregorio M, He P, McCulloch CA
Journal of cell science130(13)2196-2208(2017 Jul)

Flightless I Expression Enhances Murine Claw Regeneration Following Digit Amputation.
Strudwick XL, Waters JM, Cowin AJ
The Journal of investigative dermatology137(1)228-236(2017 Jan)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Flightless I: an actin-remodelling protein and an important negative regulator of wound repair.
Kopecki Z, Cowin AJ
The international journal of biochemistry & cell biology40(8)1415-9(2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Flii genetic expression is enhances tissue regeneration, after claw amputation.
Title: Flightless I Expression Enhances Murine Claw Regeneration Following Digit Amputation.

P-Rex1 stimulates migration through enhancing the interaction between Rac1 and the actin-remodelling protein.
Title: Differential Rac1 signalling by guanine nucleotide exchange factors implicates FLII in regulating Rac1-driven cell migration.

Studies suggest that Flii enhances cutaneous squamous cell carcinoma progression by decreasing apoptosis and enhancing tumor cell invasion.
Title: Cytoskeletal protein Flightless I inhibits apoptosis, enhances tumor cell invasion and promotes cutaneous squamous cell carcinoma progression.

Genes downstream from Flii, including TGF-beta1 and TGF-beta3, showed significantly altered expression confirming a functional effect of the Rhodamine-Flii small interfering RNA on gene expression
Title: In vivo delivery of functional Flightless I siRNA using layer-by-layer polymer surface modification.

FliI interacts with NMMIIA to promote cell extension formation, which enables collagen remodeling in fibroblasts.
Title: Flightless I interacts with NMMIIA to promote cell extension formation, which enables collagen remodeling.

The following Flii gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Flii cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21686	NM_022009.2 Latest version!	Mus musculus flightless I actin binding protein (Flii), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$748.30 $1069.00
OMu67755	NM_001302207.1 Latest version!	Mus musculus flightless I actin binding protein (Flii), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Flii ( NM_022009.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_071292.1 Mus musculus flightless I actin binding protein (Flii), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21686
Clone ID Related Accession (Same CDS sequence)	NM_022009.2
Accession Version	NM_022009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	protein flightless-1 homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB857715.1, AK014400.1, AK049365.1 and AW047188.1. On Oct 9, 2014 this sequence version replaced NM_022009.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCA CCGGGGTGCT GCCGTTCGTG CGCGGCGTGG ACCTCAGCGG CAACGACTTC 
AAGGGTGGCT ACTTCCCTGA GAATGTTAAG GCTATGACCA GCCTGCGATG GCTAAAGCTG 
AATCGCACAG GCCTCTGCTA CCTGCCTGAA GAACTTGCGG CTCTGCAGAA GCTGGAGCAC 
CTGTCTGTGA GCCACAACCA TCTGACCACG CTTCACGGCG AACTGTCTAG CCTGCCTTCG 
CTTCGGGCCA TTGTAGCTCG GGCCAACAGC CTGAAGAATT CCGGAGTCCC TGATGACATC 
TTCAAGCTGG ATGATCTCTC TGTTCTGGAC TTGAGCCATA ACCAGTTGAC AGAATGCCCA 
CGGGAGCTGG AGAATGCCAA GAACATGCTG GTGCTCAACC TCAGCCACAA CGGCATCGAC 
TCCATCCCCA ACCAGCTGTT CATCAACCTT ACGGACTTGC TGTACCTGGA CCTCAGTGAA 
AACCGCCTAG AGAGCCTTCC CCCTCAAATG CGCCGCCTCG TGCACCTTCA GACACTGGTG 
CTGAATGGAA ACCCGCTGCT ACATGCACAG CTCCGGCAGC TCCCAGCCAT GATGGCCCTA 
CAGACCCTGC ATCTGAGGAA CACTCAACGC ACCCAGAGCA ACCTCCCCAC CAGCCTGGAG 
GGCCTGAGCA ACCTTTCAGA TGTGGACCTG TCGTGCAATG ACTTGACAAG GGTGCCGGAG 
TGCCTGTACA CCCTCCCCAG CCTGCGCCGC CTCAACCTCA GTAGCAACCA GATTGCAGAG 
CTGTCCCTGT GCATCGACCA GTGGGTGCAC CTGGAGACCT TGAATCTGTC CCGGAACCAG 
CTCACCTCCC TGCCTTCAGC CATCTGCAAG CTCACCAAGC TGAAGAAGCT GTACCTCAAC 
TCCAACAAGC TGGACTTTGA TGGGCTGCCT TCTGGCATCG GCAAGCTCAC CAGCCTGGAG 
GAATTCATGG CTGCCAACAA CAACCTAGAA CTGATTCCTG AAAGTCTCTG CAGGTGCCCA 
AAACTGAAGA AACTCGTCCT GAATAAGAAC CGCCTGGTGA CCCTTCCTGA GGCCATCCAC 
TTCCTGACAG AGATCCAGGT CCTGGATGTT CGAGAAAACC CCAGCTTGGT CATGCCGCCC 
AAGCCTGCTG ACCGCACCGC CGAGTGGTAC AACATTGACT TCTCGCTACA GAACCAGCTG 
AGGCTGGCGG GCGCCTCCCC GGCCACAGTG GCTGCTGCTG CAGCTGTAGG GAGCGGGTCC 
AAGGACCCCT TGGCTCGCAA GATGCGGCTA CGGAGGCGCA AGGACTCAGC ACAGGATGTC 
CAGGCCAAGC AAGTGCTAAA GGGCATGTCA GATGTTGCCC AGGAGAAAAA CAAAAACCAA 
GAGGAAAGCA TAGATGCCCG GGCACCAGGG GGAAAGGTAC GACGCTGGGA CCAGGGCCTG 
GAGAAGCCAC GCCTTGACTA CTCGGAGTTC TTTACGGAGG ATGTGGGCCA GCTGCCTGGT 
TTGACCATCT GGCAAATTGA GAACTTTGTG CCTGTTCTGG TGGAAGAAGC CTTCCATGGC 
AAGTTCTACG AGGCTGACTG CTACATTGTG CTCAAGACCT TCCTGGATGA CAGCGGCTCC 
CTGAACTGGG AGATCTACTA CTGGATTGGT GGGGAGGCCA CACTTGACAA GAAAGCCTGT 
TCCGCCATTC ATGCAGTCAA TCTGCGCAAC TACCTGGGTG CTGAGTGCCG TACTGTTCGA 
GAGGAGATGG GTGACGAGAG TGAGGAGTTC CTGCAGGTGT TTGACAATGA CATCTCCTAC 
ATTGAAGGCG GAACAGCCAG TGGCTTCTAT ACTGTGGAAG ATACACACTA TGTTACCAGG 
ATGTACCGTG TATATGGGAA AAAGAACATC AAATTGGAGC CTGTGCCCCT CAAAGGGTCC 
TCACTAGATC CAAGGTTTGT GTTTCTCCTG GACCAAGGGC TGGACATTTA TGTGTGGCGG 
GGGGCCCAGG CCACACTGAG TAACACGACT AAGGCCAGGC TCTTTGCAGA GAAAATAAAC 
AAGAATGAGC GGAAAGGGAA GGCGGAAATC ACACTCCTGG TGCAGGGCCA AGAGCCCCCA 
GGGTTCTGGG ATGTGCTGGG TGGAGAGCCT TCTGAGATCA AGAATCATGT GCCTGATGAC 
TTTTGGCCAC CCCAGCCCAA GCTATACAAG GTGGGCCTGG GTCTGGGCTA CTTGGAGCTT 
CCGCAGATCA ACTACAAGCT CTCAGTGGAA CACAAAAAAC GGCCCAAGGT GGAGCTGATG 
CCAGGAATGA GGCTGCTGCA GAGCCTACTG GACACACGAT GCGTGTACAT CCTGGACTGC 
TGGTCTGATG TATTCATCTG GCTGGGCCGC AAGTCCCCAC GCCTAGTGCG TGCAGCAGCA 
CTCAAACTTG GCCAGGAGCT ATGTGGGATG CTACACCGGC CACGACATAC AGTGGTCAGC 
CGCAGCCTGG AGGGCACCGA GGCCCAGGTG TTCAAGGCCA AGTTCAAGAA CTGGGACGAT 
GTGTTGACAG TGGACTACAC ACGGAATGCA GAGGCTGTGC TTCAGGGCCA GGGCCTCTCT 
GGGAAGGTGA AGCGTGACAC TGAGAAGACA GACCAGATGA AGGCTGACCT CACTGCACTC 
TTCCTACCTC GGCAGCCCCC CATGCCCCTG GCTGAGGCAG AGCAGCTGAT GGAGGAGTGG 
AATGAGGACC TGGATGGCAT GGAGGGCTTC GTGCTGGAGG GCCGCAAGTT CACTCGACTG 
CCAGAGGAGG AGTTTGGCCA CTTCTACACA CAGGACTGCT ATGTCTTCCT CTGCAGGTAC 
TGGGTACCTG TGGAGTACGA GGAGGAAGAG AAGACAGAAG ACAAGGAGGG GAAGGCCTCG 
GCAGAGGCCA GAGAAGGGGA GGAAGCAGCA GCGGAGGCAG AGGAGAAGCA GCCAGAGGAG 
GACTTTCAGT GCATCGTTTA CTTCTGGCAG GGCCGGGAGG CCTCCAACAT GGGCTGGCTG 
ACCTTCACAT TCAGTCTTCA GAAGAAGTTT GAGAGCCTCT TCCCTGGCAA GCTGGAGGTA 
GTACGTATGA CACAGCAGCA GGAGAACCCC AAGTTCCTAT CCCATTTCAA AAGAAAGTTC 
ATCATTCACC GAGGCAAGAG GAAGGTGACC CAGGGTACTC TTCAGCCGAC CCTCTATCAG 
ATCCGCACCA ACGGCAGTGC CCTCTGCACC CGGTGCATCC AGATTAACAC TGACTCCAGC 
CTTCTCAACT CTGAGTTCTG CTTCATCCTC AAGGTCCCCT TTGAAAGCGA GGACAACCAA 
GGCATTGTGT ATGCCTGGGT AGGTCGGGCA TCAGACCCCG ACGAAGCCAA GCTGGCAGAG 
GACATCCTGA ACACTATGTT TGATGCATCC TACAGCAAAC AGGTCATCAA TGAAGGCGAG 
GAGCCAGAGA ACTTCTTCTG GGTAGGCATT GGTGCACAGA AACCCTATGA TGACGATGCG 
GAGTACATGA AGCACACGAG GCTCTTCAGG TGCTCCAATG AGAAGGGTTA CTTCGCAGTG 
ACTGAGAAAT GCTCTGACTT TTGCCAAGAT GACCTGGCAG ACGATGACAT CATGCTGCTA 
GACAATGGCC AAGAAGTCTA CATGTGGGTT GGGACCCAGA CAAGCCAAGT GGAGATCAAA 
CTGAGTCTGA AGGCTTGCCA GGTATACATC CAGCACACAC GCTCTAAAGA ACATGAGCGG 
CCACGTCGCC TGCGCCTGGT CCGCAAAGGT AATGAGCAGC GCGCCTTCAC CCGCTGCTTC 
CACGCCTGGA GCACGTTCCG CCAAGCCCCA GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071292.1
CDS	75..3890
Translation	MEATGVLPFVRGVDLSGNDFKGGYFPENVKAMTSLRWLKLNRTGLCYLPEELAALQKLEHLSVSHNHLTTLHGELSSLPSLRAIVARANSLKNSGVPDDIFKLDDLSVLDLSHNQLTECPRELENAKNMLVLNLSHNGIDSIPNQLFINLTDLLYLDLSENRLESLPPQMRRLVHLQTLVLNGNPLLHAQLRQLPAMMALQTLHLRNTQRTQSNLPTSLEGLSNLSDVDLSCNDLTRVPECLYTLPSLRRLNLSSNQIAELSLCIDQWVHLETLNLSRNQLTSLPSAICKLTKLKKLYLNSNKLDFDGLPSGIGKLTSLEEFMAANNNLELIPESLCRCPKLKKLVLNKNRLVTLPEAIHFLTEIQVLDVRENPSLVMPPKPADRTAEWYNIDFSLQNQLRLAGASPATVAAAAAVGSGSKDPLARKMRLRRRKDSAQDVQAKQVLKGMSDVAQEKNKNQEESIDARAPGGKVRRWDQGLEKPRLDYSEFFTEDVGQLPGLTIWQIENFVPVLVEEAFHGKFYEADCYIVLKTFLDDSGSLNWEIYYWIGGEATLDKKACSAIHAVNLRNYLGAECRTVREEMGDESEEFLQVFDNDISYIEGGTASGFYTVEDTHYVTRMYRVYGKKNIKLEPVPLKGSSLDPRFVFLLDQGLDIYVWRGAQATLSNTTKARLFAEKINKNERKGKAEITLLVQGQEPPGFWDVLGGEPSEIKNHVPDDFWPPQPKLYKVGLGLGYLELPQINYKLSVEHKKRPKVELMPGMRLLQSLLDTRCVYILDCWSDVFIWLGRKSPRLVRAAALKLGQELCGMLHRPRHTVVSRSLEGTEAQVFKAKFKNWDDVLTVDYTRNAEAVLQGQGLSGKVKRDTEKTDQMKADLTALFLPRQPPMPLAEAEQLMEEWNEDLDGMEGFVLEGRKFTRLPEEEFGHFYTQDCYVFLCRYWVPVEYEEEEKTEDKEGKASAEAREGEEAAAEAEEKQPEEDFQCIVYFWQGREASNMGWLTFTFSLQKKFESLFPGKLEVVRMTQQQENPKFLSHFKRKFIIHRGKRKVTQGTLQPTLYQIRTNGSALCTRCIQINTDSSLLNSEFCFILKVPFESEDNQGIVYAWVGRASDPDEAKLAEDILNTMFDASYSKQVINEGEEPENFFWVGIGAQKPYDDDAEYMKHTRLFRCSNEKGYFAVTEKCSDFCQDDLADDDIMLLDNGQEVYMWVGTQTSQVEIKLSLKACQVYIQHTRSKEHERPRRLRLVRKGNEQRAFTRCFHAWSTFRQAPA

Target ORF information:

RefSeq Version	NM_022009.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus flightless I actin binding protein (Flii), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022009.2

ORF Insert Sequence:

ATGGAGGCCA CCGGGGTGCT GCCGTTCGTG CGCGGCGTGG ACCTCAGCGG CAACGACTTC 
AAGGGTGGCT ACTTCCCTGA GAATGTTAAG GCTATGACCA GCCTGCGATG GCTAAAGCTG 
AATCGCACAG GCCTCTGCTA CCTGCCTGAA GAACTTGCGG CTCTGCAGAA GCTGGAGCAC 
CTGTCTGTGA GCCACAACCA TCTGACCACG CTTCACGGCG AACTGTCTAG CCTGCCTTCG 
CTTCGGGCCA TTGTAGCTCG GGCCAACAGC CTGAAGAATT CCGGAGTCCC TGATGACATC 
TTCAAGCTGG ATGATCTCTC TGTTCTGGAC TTGAGCCATA ACCAGTTGAC AGAATGCCCA 
CGGGAGCTGG AGAATGCCAA GAACATGCTG GTGCTCAACC TCAGCCACAA CGGCATCGAC 
TCCATCCCCA ACCAGCTGTT CATCAACCTT ACGGACTTGC TGTACCTGGA CCTCAGTGAA 
AACCGCCTAG AGAGCCTTCC CCCTCAAATG CGCCGCCTCG TGCACCTTCA GACACTGGTG 
CTGAATGGAA ACCCGCTGCT ACATGCACAG CTCCGGCAGC TCCCAGCCAT GATGGCCCTA 
CAGACCCTGC ATCTGAGGAA CACTCAACGC ACCCAGAGCA ACCTCCCCAC CAGCCTGGAG 
GGCCTGAGCA ACCTTTCAGA TGTGGACCTG TCGTGCAATG ACTTGACAAG GGTGCCGGAG 
TGCCTGTACA CCCTCCCCAG CCTGCGCCGC CTCAACCTCA GTAGCAACCA GATTGCAGAG 
CTGTCCCTGT GCATCGACCA GTGGGTGCAC CTGGAGACCT TGAATCTGTC CCGGAACCAG 
CTCACCTCCC TGCCTTCAGC CATCTGCAAG CTCACCAAGC TGAAGAAGCT GTACCTCAAC 
TCCAACAAGC TGGACTTTGA TGGGCTGCCT TCTGGCATCG GCAAGCTCAC CAGCCTGGAG 
GAATTCATGG CTGCCAACAA CAACCTAGAA CTGATTCCTG AAAGTCTCTG CAGGTGCCCA 
AAACTGAAGA AACTCGTCCT GAATAAGAAC CGCCTGGTGA CCCTTCCTGA GGCCATCCAC 
TTCCTGACAG AGATCCAGGT CCTGGATGTT CGAGAAAACC CCAGCTTGGT CATGCCGCCC 
AAGCCTGCTG ACCGCACCGC CGAGTGGTAC AACATTGACT TCTCGCTACA GAACCAGCTG 
AGGCTGGCGG GCGCCTCCCC GGCCACAGTG GCTGCTGCTG CAGCTGTAGG GAGCGGGTCC 
AAGGACCCCT TGGCTCGCAA GATGCGGCTA CGGAGGCGCA AGGACTCAGC ACAGGATGTC 
CAGGCCAAGC AAGTGCTAAA GGGCATGTCA GATGTTGCCC AGGAGAAAAA CAAAAACCAA 
GAGGAAAGCA TAGATGCCCG GGCACCAGGG GGAAAGGTAC GACGCTGGGA CCAGGGCCTG 
GAGAAGCCAC GCCTTGACTA CTCGGAGTTC TTTACGGAGG ATGTGGGCCA GCTGCCTGGT 
TTGACCATCT GGCAAATTGA GAACTTTGTG CCTGTTCTGG TGGAAGAAGC CTTCCATGGC 
AAGTTCTACG AGGCTGACTG CTACATTGTG CTCAAGACCT TCCTGGATGA CAGCGGCTCC 
CTGAACTGGG AGATCTACTA CTGGATTGGT GGGGAGGCCA CACTTGACAA GAAAGCCTGT 
TCCGCCATTC ATGCAGTCAA TCTGCGCAAC TACCTGGGTG CTGAGTGCCG TACTGTTCGA 
GAGGAGATGG GTGACGAGAG TGAGGAGTTC CTGCAGGTGT TTGACAATGA CATCTCCTAC 
ATTGAAGGCG GAACAGCCAG TGGCTTCTAT ACTGTGGAAG ATACACACTA TGTTACCAGG 
ATGTACCGTG TATATGGGAA AAAGAACATC AAATTGGAGC CTGTGCCCCT CAAAGGGTCC 
TCACTAGATC CAAGGTTTGT GTTTCTCCTG GACCAAGGGC TGGACATTTA TGTGTGGCGG 
GGGGCCCAGG CCACACTGAG TAACACGACT AAGGCCAGGC TCTTTGCAGA GAAAATAAAC 
AAGAATGAGC GGAAAGGGAA GGCGGAAATC ACACTCCTGG TGCAGGGCCA AGAGCCCCCA 
GGGTTCTGGG ATGTGCTGGG TGGAGAGCCT TCTGAGATCA AGAATCATGT GCCTGATGAC 
TTTTGGCCAC CCCAGCCCAA GCTATACAAG GTGGGCCTGG GTCTGGGCTA CTTGGAGCTT 
CCGCAGATCA ACTACAAGCT CTCAGTGGAA CACAAAAAAC GGCCCAAGGT GGAGCTGATG 
CCAGGAATGA GGCTGCTGCA GAGCCTACTG GACACACGAT GCGTGTACAT CCTGGACTGC 
TGGTCTGATG TATTCATCTG GCTGGGCCGC AAGTCCCCAC GCCTAGTGCG TGCAGCAGCA 
CTCAAACTTG GCCAGGAGCT ATGTGGGATG CTACACCGGC CACGACATAC AGTGGTCAGC 
CGCAGCCTGG AGGGCACCGA GGCCCAGGTG TTCAAGGCCA AGTTCAAGAA CTGGGACGAT 
GTGTTGACAG TGGACTACAC ACGGAATGCA GAGGCTGTGC TTCAGGGCCA GGGCCTCTCT 
GGGAAGGTGA AGCGTGACAC TGAGAAGACA GACCAGATGA AGGCTGACCT CACTGCACTC 
TTCCTACCTC GGCAGCCCCC CATGCCCCTG GCTGAGGCAG AGCAGCTGAT GGAGGAGTGG 
AATGAGGACC TGGATGGCAT GGAGGGCTTC GTGCTGGAGG GCCGCAAGTT CACTCGACTG 
CCAGAGGAGG AGTTTGGCCA CTTCTACACA CAGGACTGCT ATGTCTTCCT CTGCAGGTAC 
TGGGTACCTG TGGAGTACGA GGAGGAAGAG AAGACAGAAG ACAAGGAGGG GAAGGCCTCG 
GCAGAGGCCA GAGAAGGGGA GGAAGCAGCA GCGGAGGCAG AGGAGAAGCA GCCAGAGGAG 
GACTTTCAGT GCATCGTTTA CTTCTGGCAG GGCCGGGAGG CCTCCAACAT GGGCTGGCTG 
ACCTTCACAT TCAGTCTTCA GAAGAAGTTT GAGAGCCTCT TCCCTGGCAA GCTGGAGGTA 
GTACGTATGA CACAGCAGCA GGAGAACCCC AAGTTCCTAT CCCATTTCAA AAGAAAGTTC 
ATCATTCACC GAGGCAAGAG GAAGGTGACC CAGGGTACTC TTCAGCCGAC CCTCTATCAG 
ATCCGCACCA ACGGCAGTGC CCTCTGCACC CGGTGCATCC AGATTAACAC TGACTCCAGC 
CTTCTCAACT CTGAGTTCTG CTTCATCCTC AAGGTCCCCT TTGAAAGCGA GGACAACCAA 
GGCATTGTGT ATGCCTGGGT AGGTCGGGCA TCAGACCCCG ACGAAGCCAA GCTGGCAGAG 
GACATCCTGA ACACTATGTT TGATGCATCC TACAGCAAAC AGGTCATCAA TGAAGGCGAG 
GAGCCAGAGA ACTTCTTCTG GGTAGGCATT GGTGCACAGA AACCCTATGA TGACGATGCG 
GAGTACATGA AGCACACGAG GCTCTTCAGG TGCTCCAATG AGAAGGGTTA CTTCGCAGTG 
ACTGAGAAAT GCTCTGACTT TTGCCAAGAT GACCTGGCAG ACGATGACAT CATGCTGCTA 
GACAATGGCC AAGAAGTCTA CATGTGGGTT GGGACCCAGA CAAGCCAAGT GGAGATCAAA 
CTGAGTCTGA AGGCTTGCCA GGTATACATC CAGCACACAC GCTCTAAAGA ACATGAGCGG 
CCACGTCGCC TGCGCCTGGT CCGCAAAGGT AATGAGCAGC GCGCCTTCAC CCGCTGCTTC 
CACGCCTGGA GCACGTTCCG CCAAGCCCCA GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Flii ( NM_001302207.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289136.1 Mus musculus flightless I actin binding protein (Flii), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67755
Clone ID Related Accession (Same CDS sequence)	NM_001302207.1
Accession Version	NM_001302207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	protein flightless-1 homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB857715.1, AK014400.1, AK049365.1, CV784480.1 and AW047188.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in isoform 2, which is shorter by one amino acid, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCA CCGGGGTGCT GCCGTTCGTG CGCGGCGTGG ACCTCAGCGG CAACGACTTC 
AAGGGTGGCT ACTTCCCTGA GAATGTTAAG GCTATGACCA GCCTGCGATG GCTAAAGCTG 
AATCGCACAG GCCTCTGCTA CCTGCCTGAA GAACTTGCGG CTCTGCAGAA GCTGGAGCAC 
CTGTCTGTGA GCCACAACCA TCTGACCACG CTTCACGGCG AACTGTCTAG CCTGCCTTCG 
CTTCGGGCCA TTGTAGCTCG GGCCAACAGC CTGAAGAATT CCGGAGTCCC TGATGACATC 
TTCAAGCTGG ATGATCTCTC TGTTCTGGAC TTGAGCCATA ACCAGTTGAC AGAATGCCCA 
CGGGAGCTGG AGAATGCCAA GAACATGCTG GTGCTCAACC TCAGCCACAA CGGCATCGAC 
TCCATCCCCA ACCAGCTGTT CATCAACCTT ACGGACTTGC TGTACCTGGA CCTCAGTGAA 
AACCGCCTAG AGAGCCTTCC CCCTCAAATG CGCCGCCTCG TGCACCTTCA GACACTGGTG 
CTGAATGGAA ACCCGCTGCT ACATGCACAG CTCCGGCAGC TCCCAGCCAT GATGGCCCTA 
CAGACCCTGC ATCTGAGGAA CACTCAACGC ACCCAGAGCA ACCTCCCCAC CAGCCTGGAG 
GGCCTGAGCA ACCTTTCAGA TGTGGACCTG TCGTGCAATG ACTTGACAAG GGTGCCGGAG 
TGCCTGTACA CCCTCCCCAG CCTGCGCCGC CTCAACCTCA GTAGCAACCA GATTGCAGAG 
CTGTCCCTGT GCATCGACCA GTGGGTGCAC CTGGAGACCT TGAATCTGTC CCGGAACCAG 
CTCACCTCCC TGCCTTCAGC CATCTGCAAG CTCACCAAGC TGAAGAAGCT GTACCTCAAC 
TCCAACAAGC TGGACTTTGA TGGGCTGCCT TCTGGCATCG GCAAGCTCAC CAGCCTGGAG 
GAATTCATGG CTGCCAACAA CAACCTAGAA CTGATTCCTG AAAGTCTCTG CAGGTGCCCA 
AAACTGAAGA AACTCGTCCT GAATAAGAAC CGCCTGGTGA CCCTTCCTGA GGCCATCCAC 
TTCCTGACAG AGATCCAGGT CCTGGATGTT CGAGAAAACC CCAGCTTGGT CATGCCGCCC 
AAGCCTGCTG ACCGCACCGC CGAGTGGTAC AACATTGACT TCTCGCTACA GAACCAGCTG 
AGGCTGGCGG GCGCCTCCCC GGCCACAGTG GCTGCTGCTG CAGCTGGGAG CGGGTCCAAG 
GACCCCTTGG CTCGCAAGAT GCGGCTACGG AGGCGCAAGG ACTCAGCACA GGATGTCCAG 
GCCAAGCAAG TGCTAAAGGG CATGTCAGAT GTTGCCCAGG AGAAAAACAA AAACCAAGAG 
GAAAGCATAG ATGCCCGGGC ACCAGGGGGA AAGGTACGAC GCTGGGACCA GGGCCTGGAG 
AAGCCACGCC TTGACTACTC GGAGTTCTTT ACGGAGGATG TGGGCCAGCT GCCTGGTTTG 
ACCATCTGGC AAATTGAGAA CTTTGTGCCT GTTCTGGTGG AAGAAGCCTT CCATGGCAAG 
TTCTACGAGG CTGACTGCTA CATTGTGCTC AAGACCTTCC TGGATGACAG CGGCTCCCTG 
AACTGGGAGA TCTACTACTG GATTGGTGGG GAGGCCACAC TTGACAAGAA AGCCTGTTCC 
GCCATTCATG CAGTCAATCT GCGCAACTAC CTGGGTGCTG AGTGCCGTAC TGTTCGAGAG 
GAGATGGGTG ACGAGAGTGA GGAGTTCCTG CAGGTGTTTG ACAATGACAT CTCCTACATT 
GAAGGCGGAA CAGCCAGTGG CTTCTATACT GTGGAAGATA CACACTATGT TACCAGGATG 
TACCGTGTAT ATGGGAAAAA GAACATCAAA TTGGAGCCTG TGCCCCTCAA AGGGTCCTCA 
CTAGATCCAA GGTTTGTGTT TCTCCTGGAC CAAGGGCTGG ACATTTATGT GTGGCGGGGG 
GCCCAGGCCA CACTGAGTAA CACGACTAAG GCCAGGCTCT TTGCAGAGAA AATAAACAAG 
AATGAGCGGA AAGGGAAGGC GGAAATCACA CTCCTGGTGC AGGGCCAAGA GCCCCCAGGG 
TTCTGGGATG TGCTGGGTGG AGAGCCTTCT GAGATCAAGA ATCATGTGCC TGATGACTTT 
TGGCCACCCC AGCCCAAGCT ATACAAGGTG GGCCTGGGTC TGGGCTACTT GGAGCTTCCG 
CAGATCAACT ACAAGCTCTC AGTGGAACAC AAAAAACGGC CCAAGGTGGA GCTGATGCCA 
GGAATGAGGC TGCTGCAGAG CCTACTGGAC ACACGATGCG TGTACATCCT GGACTGCTGG 
TCTGATGTAT TCATCTGGCT GGGCCGCAAG TCCCCACGCC TAGTGCGTGC AGCAGCACTC 
AAACTTGGCC AGGAGCTATG TGGGATGCTA CACCGGCCAC GACATACAGT GGTCAGCCGC 
AGCCTGGAGG GCACCGAGGC CCAGGTGTTC AAGGCCAAGT TCAAGAACTG GGACGATGTG 
TTGACAGTGG ACTACACACG GAATGCAGAG GCTGTGCTTC AGGGCCAGGG CCTCTCTGGG 
AAGGTGAAGC GTGACACTGA GAAGACAGAC CAGATGAAGG CTGACCTCAC TGCACTCTTC 
CTACCTCGGC AGCCCCCCAT GCCCCTGGCT GAGGCAGAGC AGCTGATGGA GGAGTGGAAT 
GAGGACCTGG ATGGCATGGA GGGCTTCGTG CTGGAGGGCC GCAAGTTCAC TCGACTGCCA 
GAGGAGGAGT TTGGCCACTT CTACACACAG GACTGCTATG TCTTCCTCTG CAGGTACTGG 
GTACCTGTGG AGTACGAGGA GGAAGAGAAG ACAGAAGACA AGGAGGGGAA GGCCTCGGCA 
GAGGCCAGAG AAGGGGAGGA AGCAGCAGCG GAGGCAGAGG AGAAGCAGCC AGAGGAGGAC 
TTTCAGTGCA TCGTTTACTT CTGGCAGGGC CGGGAGGCCT CCAACATGGG CTGGCTGACC 
TTCACATTCA GTCTTCAGAA GAAGTTTGAG AGCCTCTTCC CTGGCAAGCT GGAGGTAGTA 
CGTATGACAC AGCAGCAGGA GAACCCCAAG TTCCTATCCC ATTTCAAAAG AAAGTTCATC 
ATTCACCGAG GCAAGAGGAA GGTGACCCAG GGTACTCTTC AGCCGACCCT CTATCAGATC 
CGCACCAACG GCAGTGCCCT CTGCACCCGG TGCATCCAGA TTAACACTGA CTCCAGCCTT 
CTCAACTCTG AGTTCTGCTT CATCCTCAAG GTCCCCTTTG AAAGCGAGGA CAACCAAGGC 
ATTGTGTATG CCTGGGTAGG TCGGGCATCA GACCCCGACG AAGCCAAGCT GGCAGAGGAC 
ATCCTGAACA CTATGTTTGA TGCATCCTAC AGCAAACAGG TCATCAATGA AGGCGAGGAG 
CCAGAGAACT TCTTCTGGGT AGGCATTGGT GCACAGAAAC CCTATGATGA CGATGCGGAG 
TACATGAAGC ACACGAGGCT CTTCAGGTGC TCCAATGAGA AGGGTTACTT CGCAGTGACT 
GAGAAATGCT CTGACTTTTG CCAAGATGAC CTGGCAGACG ATGACATCAT GCTGCTAGAC 
AATGGCCAAG AAGTCTACAT GTGGGTTGGG ACCCAGACAA GCCAAGTGGA GATCAAACTG 
AGTCTGAAGG CTTGCCAGGT ATACATCCAG CACACACGCT CTAAAGAACA TGAGCGGCCA 
CGTCGCCTGC GCCTGGTCCG CAAAGGTAAT GAGCAGCGCG CCTTCACCCG CTGCTTCCAC 
GCCTGGAGCA CGTTCCGCCA AGCCCCAGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289136.1
CDS	75..3887
Translation	MEATGVLPFVRGVDLSGNDFKGGYFPENVKAMTSLRWLKLNRTGLCYLPEELAALQKLEHLSVSHNHLTTLHGELSSLPSLRAIVARANSLKNSGVPDDIFKLDDLSVLDLSHNQLTECPRELENAKNMLVLNLSHNGIDSIPNQLFINLTDLLYLDLSENRLESLPPQMRRLVHLQTLVLNGNPLLHAQLRQLPAMMALQTLHLRNTQRTQSNLPTSLEGLSNLSDVDLSCNDLTRVPECLYTLPSLRRLNLSSNQIAELSLCIDQWVHLETLNLSRNQLTSLPSAICKLTKLKKLYLNSNKLDFDGLPSGIGKLTSLEEFMAANNNLELIPESLCRCPKLKKLVLNKNRLVTLPEAIHFLTEIQVLDVRENPSLVMPPKPADRTAEWYNIDFSLQNQLRLAGASPATVAAAAAGSGSKDPLARKMRLRRRKDSAQDVQAKQVLKGMSDVAQEKNKNQEESIDARAPGGKVRRWDQGLEKPRLDYSEFFTEDVGQLPGLTIWQIENFVPVLVEEAFHGKFYEADCYIVLKTFLDDSGSLNWEIYYWIGGEATLDKKACSAIHAVNLRNYLGAECRTVREEMGDESEEFLQVFDNDISYIEGGTASGFYTVEDTHYVTRMYRVYGKKNIKLEPVPLKGSSLDPRFVFLLDQGLDIYVWRGAQATLSNTTKARLFAEKINKNERKGKAEITLLVQGQEPPGFWDVLGGEPSEIKNHVPDDFWPPQPKLYKVGLGLGYLELPQINYKLSVEHKKRPKVELMPGMRLLQSLLDTRCVYILDCWSDVFIWLGRKSPRLVRAAALKLGQELCGMLHRPRHTVVSRSLEGTEAQVFKAKFKNWDDVLTVDYTRNAEAVLQGQGLSGKVKRDTEKTDQMKADLTALFLPRQPPMPLAEAEQLMEEWNEDLDGMEGFVLEGRKFTRLPEEEFGHFYTQDCYVFLCRYWVPVEYEEEEKTEDKEGKASAEAREGEEAAAEAEEKQPEEDFQCIVYFWQGREASNMGWLTFTFSLQKKFESLFPGKLEVVRMTQQQENPKFLSHFKRKFIIHRGKRKVTQGTLQPTLYQIRTNGSALCTRCIQINTDSSLLNSEFCFILKVPFESEDNQGIVYAWVGRASDPDEAKLAEDILNTMFDASYSKQVINEGEEPENFFWVGIGAQKPYDDDAEYMKHTRLFRCSNEKGYFAVTEKCSDFCQDDLADDDIMLLDNGQEVYMWVGTQTSQVEIKLSLKACQVYIQHTRSKEHERPRRLRLVRKGNEQRAFTRCFHAWSTFRQAPA

Target ORF information:

RefSeq Version	NM_001302207.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus flightless I actin binding protein (Flii), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302207.1

ORF Insert Sequence:

ATGGAGGCCA CCGGGGTGCT GCCGTTCGTG CGCGGCGTGG ACCTCAGCGG CAACGACTTC 
AAGGGTGGCT ACTTCCCTGA GAATGTTAAG GCTATGACCA GCCTGCGATG GCTAAAGCTG 
AATCGCACAG GCCTCTGCTA CCTGCCTGAA GAACTTGCGG CTCTGCAGAA GCTGGAGCAC 
CTGTCTGTGA GCCACAACCA TCTGACCACG CTTCACGGCG AACTGTCTAG CCTGCCTTCG 
CTTCGGGCCA TTGTAGCTCG GGCCAACAGC CTGAAGAATT CCGGAGTCCC TGATGACATC 
TTCAAGCTGG ATGATCTCTC TGTTCTGGAC TTGAGCCATA ACCAGTTGAC AGAATGCCCA 
CGGGAGCTGG AGAATGCCAA GAACATGCTG GTGCTCAACC TCAGCCACAA CGGCATCGAC 
TCCATCCCCA ACCAGCTGTT CATCAACCTT ACGGACTTGC TGTACCTGGA CCTCAGTGAA 
AACCGCCTAG AGAGCCTTCC CCCTCAAATG CGCCGCCTCG TGCACCTTCA GACACTGGTG 
CTGAATGGAA ACCCGCTGCT ACATGCACAG CTCCGGCAGC TCCCAGCCAT GATGGCCCTA 
CAGACCCTGC ATCTGAGGAA CACTCAACGC ACCCAGAGCA ACCTCCCCAC CAGCCTGGAG 
GGCCTGAGCA ACCTTTCAGA TGTGGACCTG TCGTGCAATG ACTTGACAAG GGTGCCGGAG 
TGCCTGTACA CCCTCCCCAG CCTGCGCCGC CTCAACCTCA GTAGCAACCA GATTGCAGAG 
CTGTCCCTGT GCATCGACCA GTGGGTGCAC CTGGAGACCT TGAATCTGTC CCGGAACCAG 
CTCACCTCCC TGCCTTCAGC CATCTGCAAG CTCACCAAGC TGAAGAAGCT GTACCTCAAC 
TCCAACAAGC TGGACTTTGA TGGGCTGCCT TCTGGCATCG GCAAGCTCAC CAGCCTGGAG 
GAATTCATGG CTGCCAACAA CAACCTAGAA CTGATTCCTG AAAGTCTCTG CAGGTGCCCA 
AAACTGAAGA AACTCGTCCT GAATAAGAAC CGCCTGGTGA CCCTTCCTGA GGCCATCCAC 
TTCCTGACAG AGATCCAGGT CCTGGATGTT CGAGAAAACC CCAGCTTGGT CATGCCGCCC 
AAGCCTGCTG ACCGCACCGC CGAGTGGTAC AACATTGACT TCTCGCTACA GAACCAGCTG 
AGGCTGGCGG GCGCCTCCCC GGCCACAGTG GCTGCTGCTG CAGCTGGGAG CGGGTCCAAG 
GACCCCTTGG CTCGCAAGAT GCGGCTACGG AGGCGCAAGG ACTCAGCACA GGATGTCCAG 
GCCAAGCAAG TGCTAAAGGG CATGTCAGAT GTTGCCCAGG AGAAAAACAA AAACCAAGAG 
GAAAGCATAG ATGCCCGGGC ACCAGGGGGA AAGGTACGAC GCTGGGACCA GGGCCTGGAG 
AAGCCACGCC TTGACTACTC GGAGTTCTTT ACGGAGGATG TGGGCCAGCT GCCTGGTTTG 
ACCATCTGGC AAATTGAGAA CTTTGTGCCT GTTCTGGTGG AAGAAGCCTT CCATGGCAAG 
TTCTACGAGG CTGACTGCTA CATTGTGCTC AAGACCTTCC TGGATGACAG CGGCTCCCTG 
AACTGGGAGA TCTACTACTG GATTGGTGGG GAGGCCACAC TTGACAAGAA AGCCTGTTCC 
GCCATTCATG CAGTCAATCT GCGCAACTAC CTGGGTGCTG AGTGCCGTAC TGTTCGAGAG 
GAGATGGGTG ACGAGAGTGA GGAGTTCCTG CAGGTGTTTG ACAATGACAT CTCCTACATT 
GAAGGCGGAA CAGCCAGTGG CTTCTATACT GTGGAAGATA CACACTATGT TACCAGGATG 
TACCGTGTAT ATGGGAAAAA GAACATCAAA TTGGAGCCTG TGCCCCTCAA AGGGTCCTCA 
CTAGATCCAA GGTTTGTGTT TCTCCTGGAC CAAGGGCTGG ACATTTATGT GTGGCGGGGG 
GCCCAGGCCA CACTGAGTAA CACGACTAAG GCCAGGCTCT TTGCAGAGAA AATAAACAAG 
AATGAGCGGA AAGGGAAGGC GGAAATCACA CTCCTGGTGC AGGGCCAAGA GCCCCCAGGG 
TTCTGGGATG TGCTGGGTGG AGAGCCTTCT GAGATCAAGA ATCATGTGCC TGATGACTTT 
TGGCCACCCC AGCCCAAGCT ATACAAGGTG GGCCTGGGTC TGGGCTACTT GGAGCTTCCG 
CAGATCAACT ACAAGCTCTC AGTGGAACAC AAAAAACGGC CCAAGGTGGA GCTGATGCCA 
GGAATGAGGC TGCTGCAGAG CCTACTGGAC ACACGATGCG TGTACATCCT GGACTGCTGG 
TCTGATGTAT TCATCTGGCT GGGCCGCAAG TCCCCACGCC TAGTGCGTGC AGCAGCACTC 
AAACTTGGCC AGGAGCTATG TGGGATGCTA CACCGGCCAC GACATACAGT GGTCAGCCGC 
AGCCTGGAGG GCACCGAGGC CCAGGTGTTC AAGGCCAAGT TCAAGAACTG GGACGATGTG 
TTGACAGTGG ACTACACACG GAATGCAGAG GCTGTGCTTC AGGGCCAGGG CCTCTCTGGG 
AAGGTGAAGC GTGACACTGA GAAGACAGAC CAGATGAAGG CTGACCTCAC TGCACTCTTC 
CTACCTCGGC AGCCCCCCAT GCCCCTGGCT GAGGCAGAGC AGCTGATGGA GGAGTGGAAT 
GAGGACCTGG ATGGCATGGA GGGCTTCGTG CTGGAGGGCC GCAAGTTCAC TCGACTGCCA 
GAGGAGGAGT TTGGCCACTT CTACACACAG GACTGCTATG TCTTCCTCTG CAGGTACTGG 
GTACCTGTGG AGTACGAGGA GGAAGAGAAG ACAGAAGACA AGGAGGGGAA GGCCTCGGCA 
GAGGCCAGAG AAGGGGAGGA AGCAGCAGCG GAGGCAGAGG AGAAGCAGCC AGAGGAGGAC 
TTTCAGTGCA TCGTTTACTT CTGGCAGGGC CGGGAGGCCT CCAACATGGG CTGGCTGACC 
TTCACATTCA GTCTTCAGAA GAAGTTTGAG AGCCTCTTCC CTGGCAAGCT GGAGGTAGTA 
CGTATGACAC AGCAGCAGGA GAACCCCAAG TTCCTATCCC ATTTCAAAAG AAAGTTCATC 
ATTCACCGAG GCAAGAGGAA GGTGACCCAG GGTACTCTTC AGCCGACCCT CTATCAGATC 
CGCACCAACG GCAGTGCCCT CTGCACCCGG TGCATCCAGA TTAACACTGA CTCCAGCCTT 
CTCAACTCTG AGTTCTGCTT CATCCTCAAG GTCCCCTTTG AAAGCGAGGA CAACCAAGGC 
ATTGTGTATG CCTGGGTAGG TCGGGCATCA GACCCCGACG AAGCCAAGCT GGCAGAGGAC 
ATCCTGAACA CTATGTTTGA TGCATCCTAC AGCAAACAGG TCATCAATGA AGGCGAGGAG 
CCAGAGAACT TCTTCTGGGT AGGCATTGGT GCACAGAAAC CCTATGATGA CGATGCGGAG 
TACATGAAGC ACACGAGGCT CTTCAGGTGC TCCAATGAGA AGGGTTACTT CGCAGTGACT 
GAGAAATGCT CTGACTTTTG CCAAGATGAC CTGGCAGACG ATGACATCAT GCTGCTAGAC 
AATGGCCAAG AAGTCTACAT GTGGGTTGGG ACCCAGACAA GCCAAGTGGA GATCAAACTG 
AGTCTGAAGG CTTGCCAGGT ATACATCCAG CACACACGCT CTAAAGAACA TGAGCGGCCA 
CGTCGCCTGC GCCTGGTCCG CAAAGGTAAT GAGCAGCGCG CCTTCACCCG CTGCTTCCAC 
GCCTGGAGCA CGTTCCGCCA AGCCCCAGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRPV4 mediates the Ca
Journal of cell science130(13)2196-2208(2017 Jul)
Arora PD,Di Gregorio M,He P,McCulloch CA

Flightless I Expression Enhances Murine Claw Regeneration Following Digit Amputation.
The Journal of investigative dermatology137(1)228-236(2017 Jan)
Strudwick XL,Waters JM,Cowin AJ

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Flightless I: an actin-remodelling protein and an important negative regulator of wound repair.
The international journal of biochemistry & cell biology40(8)1415-9(2008)
Kopecki Z,Cowin AJ

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Flii cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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