Fjx1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fjx1
Entrez Gene ID	14221
Full Name	four jointed box 1
General protein information	Preferred Names four jointed box 1 Names four-jointed box protein 1 four-jointed protein homolog four-jointed protein x1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_010218.2	NP_034348.2	four-jointed box protein 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	FJX1	NP_055159.2
Canis lupus familiaris (dog)	FJX1	XP_540543.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008093	XP_001237917.2
Pan troglodytes (chimpanzee)	FJX1	XP_508371.2
Bos taurus (cattle)	FJX1	XP_005216457.1
Danio rerio (zebrafish)	LOC100001423	XP_001337600.3
Drosophila melanogaster (fruit fly)	fj	NP_477483.1
Xenopus tropicalis (tropical clawed frog)	fjx1	XP_004913403.1
Mus musculus (house mouse)	Fjx1	NP_034348.2
Rattus norvegicus (Norway rat)	Fjx1	NP_001102425.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Fat4-Dchs1 signalling controls cell proliferation in developing vertebrae.
Kuta A, Mao Y, Martin T, Ferreira de Sousa C, Whiting D, Zakaria S, Crespo-Enriquez I, Evans P, Balczerski B, Mankoo B, Irvine KD, Francis-West PH
Development (Cambridge, England)143(13)2367-75(2016 07)

Stromal Fat4 acts non-autonomously with Dchs1/2 to restrict the nephron progenitor pool.
Bagherie-Lachidan M, Reginensi A, Pan Q, Zaveri HP, Scott DA, Blencowe BJ, Helmbacher F, McNeill H
Development (Cambridge, England)142(15)2564-73(2015 Aug)

Atypical Cadherin Fat1 Is Required for Lens Epithelial Cell Polarity and Proliferation but Not for Fiber Differentiation.
Sugiyama Y, Shelley EJ, Badouel C, McNeill H, McAvoy JW
Investigative ophthalmology & visual science56(6)4099-107(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results identify Fjx1 as a new inhibitory factor that regulates dendrite extension.
Title: The rodent Four-jointed ortholog Fjx1 regulates dendrite extension.

data are compatible with conservation and sub-functionalization of dachsous 1, Fj, and Fat signaling in higher vertebrates
Title: Expression of mouse dchs1, fjx1, and fat-j suggests conservation of the planar cell polarity pathway identified in Drosophila.

fjx1 is expressed in the peripheral nervous system, epithelial cells of multiple organs, and during limb development
Title: Fjx1: a notch-inducible secreted ligand with specific binding sites in developing mouse embryos and adult brain.

The following Fjx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fjx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18584	NM_010218.2 Latest version!	Mus musculus four jointed box 1 (Fjx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fjx1 ( NM_010218.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034348.2 Mus musculus four jointed box 1 (Fjx1), mRNA.

Price & Availability↑

CloneID	OMu18584
Clone ID Related Accession (Same CDS sequence)	NM_010218.2
Accession Version	NM_010218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	four-jointed box protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC051990.1. On May 6, 2004 this sequence version replaced NM_010218.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC051990.1 [ECO:0000345] ##Evidence-Data-END##

ATGGGGAGGA AGATGCGGGG CGCCGCCGCC GCCGCGGGGC TCTGGCTGCT GGCTTTGAGC 
TCGCTGCTGA CGCTGTGGGG AGGACTCCTG CCACCGCGGA CCGAGCTGCC AGCCTCCCGG 
CCGCCCGAAG ATCGACTCCC TCCGCATCCG ATCCAGAGTG GCGGCCCCGC GCCCGAGCCG 
CGATTCCCTC TGCCCCCGCC CCTAGTATGG GACGCCCGCG GCGGCTCCCT GAAAACTTTC 
CGGGCGCTGC TCACCCTGGC GGCCGGCGCG GATAACCCGC CTAGGAGGCA CCAGGACGAC 
CGCGGGCGGC ACGAGCCCTC CGGGCTGTCC TGGCCAGAGG AGCGCAGGGC GGTGCACGGG 
GGCGTCTTCT GGAGCCGCGG CCTGGAGGAG CAGGTTCCCC GGGGCTTTTC CGAAGCCCAA 
GCAGCAGCGT GGCTGGAGGT GGCACGGGGT GCTCGGGTGG TGGCTCTGGA TCGCGGGGGC 
TGCGGACGCA GTTCCAACCG CCTAGCCCGC TTTGCCGACG GCACCCGTGC CTGTGTACGC 
TACGGCATCA ACCCAGAGCA GATACAGGGC GAGGCCCTGT CCTACTACCT TGCGCGCCTG 
CTGGGCCTCC AGCGCCACGT GCCGCCGCTG GCACTGGCTC GGGTGGAGGC TCGGGGCGCG 
CAGTGGGTGC AGGTGCAGGA GGAGCTGCGC ACCGCGCACT GGACCGAGGG CAGCGTGGTG 
AGCCTGACGC GCTGGCTGCC TAACCTCACC GACGTGGTGG TGCCCGAGCC CTGGCGATCA 
GAGGACGGCC GTCTGCGGCC CCTGCGCGAC GCCGGGGGCG AGCTGACCAA CCTCAGCCAG 
GCGGAGCTGG TGGACTTGGT ACAATGGACC GATCTGATCC TCTTCGATTA CCTGACGGCC 
AACTTTGACC GGCTTGTGAG CAACCTCTTC AGCTTACAGT GGGACCCACG CGTTATGCAC 
CGCGCTACGA GCAACCTGCA CCGAGGACCA GGAGGGGCGT TGGTCTTTCT GGACAATGAG 
GCGGGCTTGG TGCACGGCTA CCGGGTAGCC GGCATGTGGG ACAAGTATAA CGAACCGCTG 
CTACAGTCGG TGTGTGTATT CCGAGAGCGG ACTGCTAGGC GCGTCTTGGA GCTGCACCGG 
GGTCAGGACG CGGCGGCCCG GTTGCTGCGC CTCTACAGTC GCCACGAACC GCGTTTCCCA 
GAGCTGGCGG AGCTCTCAGA ACCCCACGCT CAGCTGCTAC AGCGCCGCCT TGACTTCCTC 
GCCAAACACA TTTTGCACTG CAAGGCCAAG TACGGCCGCC GGCCCGGGGA CTTAATAACA 
CTCCGAGGAA GAGAGGGACT GGGGTATGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034348.2
CDS	205..1557
Translation	MGRKMRGAAAAAGLWLLALSSLLTLWGGLLPPRTELPASRPPEDRLPPHPIQSGGPAPEPRFPLPPPLVWDARGGSLKTFRALLTLAAGADNPPRRHQDDRGRHEPSGLSWPEERRAVHGGVFWSRGLEEQVPRGFSEAQAAAWLEVARGARVVALDRGGCGRSSNRLARFADGTRACVRYGINPEQIQGEALSYYLARLLGLQRHVPPLALARVEARGAQWVQVQEELRTAHWTEGSVVSLTRWLPNLTDVVVPEPWRSEDGRLRPLRDAGGELTNLSQAELVDLVQWTDLILFDYLTANFDRLVSNLFSLQWDPRVMHRATSNLHRGPGGALVFLDNEAGLVHGYRVAGMWDKYNEPLLQSVCVFRERTARRVLELHRGQDAAARLLRLYSRHEPRFPELAELSEPHAQLLQRRLDFLAKHILHCKAKYGRRPGDLITLRGREGLGYE

Target ORF information:

RefSeq Version	NM_010218.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus four jointed box 1 (Fjx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010218.2

ORF Insert Sequence:

ATGGGGAGGA AGATGCGGGG CGCCGCCGCC GCCGCGGGGC TCTGGCTGCT GGCTTTGAGC 
TCGCTGCTGA CGCTGTGGGG AGGACTCCTG CCACCGCGGA CCGAGCTGCC AGCCTCCCGG 
CCGCCCGAAG ATCGACTCCC TCCGCATCCG ATCCAGAGTG GCGGCCCCGC GCCCGAGCCG 
CGATTCCCTC TGCCCCCGCC CCTAGTATGG GACGCCCGCG GCGGCTCCCT GAAAACTTTC 
CGGGCGCTGC TCACCCTGGC GGCCGGCGCG GATAACCCGC CTAGGAGGCA CCAGGACGAC 
CGCGGGCGGC ACGAGCCCTC CGGGCTGTCC TGGCCAGAGG AGCGCAGGGC GGTGCACGGG 
GGCGTCTTCT GGAGCCGCGG CCTGGAGGAG CAGGTTCCCC GGGGCTTTTC CGAAGCCCAA 
GCAGCAGCGT GGCTGGAGGT GGCACGGGGT GCTCGGGTGG TGGCTCTGGA TCGCGGGGGC 
TGCGGACGCA GTTCCAACCG CCTAGCCCGC TTTGCCGACG GCACCCGTGC CTGTGTACGC 
TACGGCATCA ACCCAGAGCA GATACAGGGC GAGGCCCTGT CCTACTACCT TGCGCGCCTG 
CTGGGCCTCC AGCGCCACGT GCCGCCGCTG GCACTGGCTC GGGTGGAGGC TCGGGGCGCG 
CAGTGGGTGC AGGTGCAGGA GGAGCTGCGC ACCGCGCACT GGACCGAGGG CAGCGTGGTG 
AGCCTGACGC GCTGGCTGCC TAACCTCACC GACGTGGTGG TGCCCGAGCC CTGGCGATCA 
GAGGACGGCC GTCTGCGGCC CCTGCGCGAC GCCGGGGGCG AGCTGACCAA CCTCAGCCAG 
GCGGAGCTGG TGGACTTGGT ACAATGGACC GATCTGATCC TCTTCGATTA CCTGACGGCC 
AACTTTGACC GGCTTGTGAG CAACCTCTTC AGCTTACAGT GGGACCCACG CGTTATGCAC 
CGCGCTACGA GCAACCTGCA CCGAGGACCA GGAGGGGCGT TGGTCTTTCT GGACAATGAG 
GCGGGCTTGG TGCACGGCTA CCGGGTAGCC GGCATGTGGG ACAAGTATAA CGAACCGCTG 
CTACAGTCGG TGTGTGTATT CCGAGAGCGG ACTGCTAGGC GCGTCTTGGA GCTGCACCGG 
GGTCAGGACG CGGCGGCCCG GTTGCTGCGC CTCTACAGTC GCCACGAACC GCGTTTCCCA 
GAGCTGGCGG AGCTCTCAGA ACCCCACGCT CAGCTGCTAC AGCGCCGCCT TGACTTCCTC 
GCCAAACACA TTTTGCACTG CAAGGCCAAG TACGGCCGCC GGCCCGGGGA CTTAATAACA 
CTCCGAGGAA GAGAGGGACT GGGGTATGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Fat4-Dchs1 signalling controls cell proliferation in developing vertebrae.
Development (Cambridge, England)143(13)2367-75(2016 07)
Kuta A,Mao Y,Martin T,Ferreira de Sousa C,Whiting D,Zakaria S,Crespo-Enriquez I,Evans P,Balczerski B,Mankoo B,Irvine KD,Francis-West PH

Stromal Fat4 acts non-autonomously with Dchs1/2 to restrict the nephron progenitor pool.
Development (Cambridge, England)142(15)2564-73(2015 Aug)
Bagherie-Lachidan M,Reginensi A,Pan Q,Zaveri HP,Scott DA,Blencowe BJ,Helmbacher F,McNeill H

Atypical Cadherin Fat1 Is Required for Lens Epithelial Cell Polarity and Proliferation but Not for Fiber Differentiation.
Investigative ophthalmology & visual science56(6)4099-107(2015 Jun)
Sugiyama Y,Shelley EJ,Badouel C,McNeill H,McAvoy JW

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Fjx1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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