Fh1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fh1
Entrez Gene ID	14194
Full Name	fumarate hydratase 1
Synonyms	Fh,Fh-1
General protein information	Preferred Names fumarate hydratase 1 Names fumarate hydratase, mitochondrial EF-3 fumarase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H3

mRNA and Protein(s)

mRNA	Protein	Name
NM_010209.2	NP_034339.2	fumarate hydratase, mitochondrial precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	FH	XP_537215.1
Mus musculus (house mouse)	Fh1	NP_034339.2
Danio rerio (zebrafish)	fh	NP_957257.1
Drosophila melanogaster (fruit fly)	l(1)G0255	NP_572339.1
Arabidopsis thaliana (thale cress)	FUM2	NP_199908.1
Xenopus tropicalis (tropical clawed frog)	LOC100491219	XP_002935862.2
	FH	XP_001094968.1
Magnaporthe oryzae (rice blast fungus)	MGG_17962	XP_003720956.1
Pan troglodytes (chimpanzee)	FH	XP_001159979.1
Rattus norvegicus (Norway rat)	Fh	NP_058701.2
Saccharomyces cerevisiae (baker's yeast)	FUM1	NP_015061.1
Kluyveromyces lactis	KLLA0D00902g	XP_453112.1
Eremothecium gossypii	AGOS_ACR013C	NP_983416.2
Schizosaccharomyces pombe (fission yeast)	fum1	NP_588397.3
Arabidopsis thaliana (thale cress)	FUM1	NP_001078075.1
Homo sapiens (human)	FH	NP_000134.2
Bos taurus (cattle)	FH	NP_001069271.1
Gallus gallus (chicken)	FH	NP_001006382.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001884	XP_321179.4
Caenorhabditis elegans (roundworm)	fum-1	NP_498642.1
Neurospora crassa	NCU10008	XP_959454.1

Gene Ontology
Bibliography

Related articles in PubMed

Fumarate Hydratase Deletion in Pancreatic ? Cells Leads to Progressive Diabetes.
Adam J, Ramracheya R, Chibalina MV, Ternette N, Hamilton A, Tarasov AI, Zhang Q, Rebelato E, Rorsman NJG, Mart?n-Del-R?o R, Lewis A, ?zkan G, Do HW, Sp?gel P, Saitoh K, Kato K, Igarashi K, Kessler BM, Pugh CW, Tamarit-Rodriguez J, Mulder H, Clark A, Frizzell N, Soga T, Ashcroft FM, Silver A, Pollard PJ, Rorsman P
Cell reports20(13)3135-3148(2017 Sep)

Fumarate hydratase is a critical metabolic regulator of hematopoietic stem cell functions.
Guitart AV, Panagopoulou TI, Villacreces A, Vukovic M, Sepulveda C, Allen L, Carter RN, van de Lagemaat LN, Morgan M, Giles P, Sas Z, Gonzalez MV, Lawson H, Paris J, Edwards-Hicks J, Schaak K, Subramani C, Gezer D, Armesilla-Diaz A, Wills J, Easterbrook A, Coman D, So CW, O'Carroll D, Vernimmen D, Rodrigues NP, Pollard PJ, Morton NM, Finch A, Kranc KR
The Journal of experimental medicine214(3)719-735(2017 Mar)

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition.
Sciacovelli M, Gon?alves E, Johnson TI, Zecchini VR, da Costa AS, Gaude E, Drubbel AV, Theobald SJ, Abbo SR, Tran MG, Rajeeve V, Cardaci S, Foster S, Yun H, Cutillas P, Warren A, Gnanapragasam V, Gottlieb E, Franze K, Huntly B, Maher ER, Maxwell PH, Saez-Rodriguez J, Frezza C
Nature537(7621)544-547(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

roles for fumarate metabolism in hematopoietic stem cell (HSC) maintenance and hematopoietic differentiation and reveal a differential requirement for mitochondrial Fh1 in normal hematopoiesis and leukemia propagation.
Title: Fumarate hydratase is a critical metabolic regulator of hematopoietic stem cell functions.

Adipose-Specific Deficiency of Fumarate Hydratase in Mice Protects Against Obesity, Hepatic Steatosis, and Insulin Resistance.
Title: Adipose-Specific Deficiency of Fumarate Hydratase in Mice Protects Against Obesity, Hepatic Steatosis, and Insulin Resistance.

Reexpression of cytosolic FH in FH1-deficient mice is critical for the suppression of renal cyst development and restoration of defects in the arginine biosynthesis pathway.
Title: A role for cytosolic fumarate hydratase in urea cycle metabolism and renal neoplasia.

study found that loss of FH activity conferred protection from apoptosis in normal human renal cells and fibroblasts
Title: Cells lacking the fumarase tumor suppressor are protected from apoptosis through a hypoxia-inducible factor-independent, AMPK-dependent mechanism.

Tumor-derived FH and SDH mutations accumulate fumarate and succinate, leading to enzymatic inhibition of multiple alpha-KG-dependent dioxygenases and consequent alterations of genome-wide histone and DNA methylation.
Title: Inhibition of ?-KG-dependent histone and DNA demethylases by fumarate and succinate that are accumulated in mutations of FH and SDH tumor suppressors.

The following Fh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15298	NM_010209.2 Latest version!	Mus musculus fumarate hydratase 1 (Fh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fh1 ( NM_010209.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034339.2 Mus musculus fumarate hydratase 1 (Fh1), mRNA.

Price & Availability↑

CloneID	OMu15298
Clone ID Related Accession (Same CDS sequence)	NM_010209.2
Accession Version	NM_010209.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Mus musculus(house mouse)
Product	fumarate hydratase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU526396.1, AK147000.1 and BQ174129.1. On Apr 15, 2009 this sequence version replaced NM_010209.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006048.1, AK147000.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGTACCGCG CACTCCGTCT CCTCGCGCGC TCGCGTCGCC TCCTGCGGGT TCCGTCCGCC 
GGTGCTGCAG TGTCTGGGGA AGCGACCACC CTCCCGCGGT GTGCTCCGAA CGTCGCGCGA 
ATGGCAAGCC AAAATTCCTT CCGTGTAGAG TTCGACACCT TTGGTGAATT GAAGGTTCCA 
ACCGATAAGT ATTATGGTGC TCAGACCGTA AGATCTACGA TGAACTTTAA GATTGGAGGT 
GCTACGGAAC GTATGCCAAT CCCAGTCATT CAAGCTTTCG GCATCTTGAA GCGAGCTGCT 
GCTGAAGTAA ACCAGGAGTA TGGTCTCGAT CCAAAGATTG CTAGTGCAAT AATGAAGGCC 
GCAGATGAGG TAGCTGAAGG TAAATTAAAT GATCACTTTC CTCTGGTGGT TTGGCAGACT 
GGATCAGGAA CCCAGACAAA CATGAATGTA AATGAAGTCA TTAGCAACAG AGCAATTGAA 
ATGTTAGGAG GTGAACTTGG CAGCAAGAAG CCTGTGCACC CCAATGATCA TGTTAACAAA 
AGCCAGAGCT CGAATGACAC CTTTCCCACA GCAATGCATA TTGCTGCTGC AGTGGAAGTT 
CACAAGGTCC TGTTGCCAGG GTTACAGAAG CTCCATGATG CTCTCAGTGC AAAATCCAAA 
GAGTTTGCGC AGGTCATCAA AATTGGGCGA ACTCACACGC AGGATGCTGT CCCTCTTACT 
CTTGGACAGG AATTCAGTGG TTACGTTCAG CAAGTCCAGT ATGCGATGGT GAGAATAAAA 
GCCGCCATGC CAAGAATCTA CGAGCTCGCT GCTGGAGGCA CTGCTGTGGG GACGGGGTTA 
AACACCAGGA TCGGTTTCGC AGAAAAGGTG GCCGCAAAAG TAGCAGCACT CACAGGTTTG 
CCTTTTGTCA CTGCCCCGAA TAAGTTTGAA GCTCTGGCTG CTCATGATGC TCTGGTTGAG 
CTTAGTGGAG CCATGAACAC TGCCGCCTGC AGTCTAATGA AGATAGCGAA TGATATTCGC 
TTCCTGGGTT CTGGTCCTCG GTCAGGTTTG GGAGAGCTGA TCTTGCCTGA AAACGAGCCA 
GGGAGCAGCA TCATGCCAGG AAAGGTGAAC CCTACTCAGT GTGAAGCGAT GACCATGGTT 
GCAGCCCAAG TCATGGGGAA TCACGTTGCT GTTACCGTTG GAGGCAGCAA TGGACATTTT 
GAACTGAATG TGTTTAAACC AATGATGATT AAGAATGTGC TGCACTCAGC CAGGCTACTG 
GGAGATGCTT CAGTGTCCTT CACAGACAAC TGTGTGGTCG GGATCCAGGC CAACACAGAG 
CGGATCAACA AGCTAATGAA TGAGTCTTTA ATGTTGGTCA CAGCTCTTAA TCCACATATA 
GGGTATGACA AAGCAGCAAA GATTGCCAAG ACCGCACACA AGAACGGATC AACCTTAAAG 
GAAACGGCTA TTGAACTTGG CTATCTCACA GCAGAGCAGT TTGATGAGTG GGTGAAACCC 
AAGGATATGC TGGGTCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034339.2
CDS	63..1586
Translation	MYRALRLLARSRRLLRVPSAGAAVSGEATTLPRCAPNVARMASQNSFRVEFDTFGELKVPTDKYYGAQTVRSTMNFKIGGATERMPIPVIQAFGILKRAAAEVNQEYGLDPKIASAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKKPVHPNDHVNKSQSSNDTFPTAMHIAAAVEVHKVLLPGLQKLHDALSAKSKEFAQVIKIGRTHTQDAVPLTLGQEFSGYVQQVQYAMVRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTAACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTDNCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

Target ORF information:

RefSeq Version	NM_010209.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus fumarate hydratase 1 (Fh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010209.2

ORF Insert Sequence:

ATGTACCGCG CACTCCGTCT CCTCGCGCGC TCGCGTCGCC TCCTGCGGGT TCCGTCCGCC 
GGTGCTGCAG TGTCTGGGGA AGCGACCACC CTCCCGCGGT GTGCTCCGAA CGTCGCGCGA 
ATGGCAAGCC AAAATTCCTT CCGTGTAGAG TTCGACACCT TTGGTGAATT GAAGGTTCCA 
ACCGATAAGT ATTATGGTGC TCAGACCGTA AGATCTACGA TGAACTTTAA GATTGGAGGT 
GCTACGGAAC GTATGCCAAT CCCAGTCATT CAAGCTTTCG GCATCTTGAA GCGAGCTGCT 
GCTGAAGTAA ACCAGGAGTA TGGTCTCGAT CCAAAGATTG CTAGTGCAAT AATGAAGGCC 
GCAGATGAGG TAGCTGAAGG TAAATTAAAT GATCACTTTC CTCTGGTGGT TTGGCAGACT 
GGATCAGGAA CCCAGACAAA CATGAATGTA AATGAAGTCA TTAGCAACAG AGCAATTGAA 
ATGTTAGGAG GTGAACTTGG CAGCAAGAAG CCTGTGCACC CCAATGATCA TGTTAACAAA 
AGCCAGAGCT CGAATGACAC CTTTCCCACA GCAATGCATA TTGCTGCTGC AGTGGAAGTT 
CACAAGGTCC TGTTGCCAGG GTTACAGAAG CTCCATGATG CTCTCAGTGC AAAATCCAAA 
GAGTTTGCGC AGGTCATCAA AATTGGGCGA ACTCACACGC AGGATGCTGT CCCTCTTACT 
CTTGGACAGG AATTCAGTGG TTACGTTCAG CAAGTCCAGT ATGCGATGGT GAGAATAAAA 
GCCGCCATGC CAAGAATCTA CGAGCTCGCT GCTGGAGGCA CTGCTGTGGG GACGGGGTTA 
AACACCAGGA TCGGTTTCGC AGAAAAGGTG GCCGCAAAAG TAGCAGCACT CACAGGTTTG 
CCTTTTGTCA CTGCCCCGAA TAAGTTTGAA GCTCTGGCTG CTCATGATGC TCTGGTTGAG 
CTTAGTGGAG CCATGAACAC TGCCGCCTGC AGTCTAATGA AGATAGCGAA TGATATTCGC 
TTCCTGGGTT CTGGTCCTCG GTCAGGTTTG GGAGAGCTGA TCTTGCCTGA AAACGAGCCA 
GGGAGCAGCA TCATGCCAGG AAAGGTGAAC CCTACTCAGT GTGAAGCGAT GACCATGGTT 
GCAGCCCAAG TCATGGGGAA TCACGTTGCT GTTACCGTTG GAGGCAGCAA TGGACATTTT 
GAACTGAATG TGTTTAAACC AATGATGATT AAGAATGTGC TGCACTCAGC CAGGCTACTG 
GGAGATGCTT CAGTGTCCTT CACAGACAAC TGTGTGGTCG GGATCCAGGC CAACACAGAG 
CGGATCAACA AGCTAATGAA TGAGTCTTTA ATGTTGGTCA CAGCTCTTAA TCCACATATA 
GGGTATGACA AAGCAGCAAA GATTGCCAAG ACCGCACACA AGAACGGATC AACCTTAAAG 
GAAACGGCTA TTGAACTTGG CTATCTCACA GCAGAGCAGT TTGATGAGTG GGTGAAACCC 
AAGGATATGC TGGGTCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fumarate Hydratase Deletion in Pancreatic ? Cells Leads to Progressive Diabetes.
Cell reports20(13)3135-3148(2017 Sep)
Adam J,Ramracheya R,Chibalina MV,Ternette N,Hamilton A,Tarasov AI,Zhang Q,Rebelato E,Rorsman NJG,Mart?n-Del-R?o R,Lewis A,?zkan G,Do HW,Sp?gel P,Saitoh K,Kato K,Igarashi K,Kessler BM,Pugh CW,Tamarit-Rodriguez J,Mulder H,Clark A,Frizzell N,Soga T,Ashcroft FM,Silver A,Pollard PJ,Rorsman P

Fumarate hydratase is a critical metabolic regulator of hematopoietic stem cell functions.
The Journal of experimental medicine214(3)719-735(2017 Mar)
Guitart AV,Panagopoulou TI,Villacreces A,Vukovic M,Sepulveda C,Allen L,Carter RN,van de Lagemaat LN,Morgan M,Giles P,Sas Z,Gonzalez MV,Lawson H,Paris J,Edwards-Hicks J,Schaak K,Subramani C,Gezer D,Armesilla-Diaz A,Wills J,Easterbrook A,Coman D,So CW,O'Carroll D,Vernimmen D,Rodrigues NP,Pollard PJ,Morton NM,Finch A,Kranc KR

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition.
Nature537(7621)544-547(2016 08)
Sciacovelli M,Gon?alves E,Johnson TI,Zecchini VR,da Costa AS,Gaude E,Drubbel AV,Theobald SJ,Abbo SR,Tran MG,Rajeeve V,Cardaci S,Foster S,Yun H,Cutillas P,Warren A,Gnanapragasam V,Gottlieb E,Franze K,Huntly B,Maher ER,Maxwell PH,Saez-Rodriguez J,Frezza C

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Fh1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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