Akr1b8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akr1b8
Entrez Gene ID	14187
Full Name	aldo-keto reductase family 1, member B8
Synonyms	FR-1,Fgfrp,Fgrp
General protein information	Preferred Names aldo-keto reductase family 1, member B8 Names aldose reductase-related protein 2 AR aldehyde reductase fibroblast growth factor-regulated protein protein FR-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 B1\|6 14.91 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008012.2	NP_032038.1	aldose reductase-related protein 2
NM_008012.1	NP_032038.1	aldose reductase-related protein 2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG6084	NP_001261717.1
Magnaporthe oryzae (rice blast fungus)	MGG_03648	XP_003716240.1
Pan troglodytes (chimpanzee)	AKR1B10	XP_001140450.1
Rattus norvegicus (Norway rat)	Akr1b8	NP_775159.1
	AKR1B10	XP_001100959.1
Bos taurus (cattle)	AKR1B10	NP_001095558.1
Mus musculus (house mouse)	Akr1b8	NP_032038.1
Gallus gallus (chicken)	AKR1B10	NP_989960.1
Saccharomyces cerevisiae (baker's yeast)	GRE3	NP_011972.1
Kluyveromyces lactis	KLLA0E21627g	XP_454929.1
Arabidopsis thaliana (thale cress)	AT2G21260	NP_179722.1
Homo sapiens (human)	AKR1B10	NP_064695.3
Eremothecium gossypii	AGOS_ACL107C	NP_983297.1
Neurospora crassa	NCU08384	XP_963931.1
Arabidopsis thaliana (thale cress)	AT2G21250	NP_179721.1
Oryza sativa (rice)	Os02g0123500	NP_001045735.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Butylated hydroxyanisole induces distinct expression patterns of Nrf2 and detoxification enzymes in the liver and small intestine of C57BL/6 mice.
Luo L, Chen Y, Wu D, Shou J, Wang S, Ye J, Tang X, Wang XJ
Toxicology and applied pharmacology288(3)339-48(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We defined the basal and butylated hydroxyanisole induced expression patterns of Nqo1, AKR1B8, and Ho-1 in the liver and small intestine of C57BL/6 mice.
Title: Butylated hydroxyanisole induces distinct expression patterns of Nrf2 and detoxification enzymes in the liver and small intestine of C57BL/6 mice.

AKR1B8 is a critical protein in the proliferation and injury repair of the colonic epithelium and in the pathogenesis of ulcerative colitis and colitis-associated colorectal cancer, being a new etiopathogenic factor of these diseases.
Title: Impaired self-renewal and increased colitis and dysplastic lesions in colonic mucosa of AKR1B8-deficient mice.

AKR1B8 is a new secretory protein through the lysosomes-mediated non-classical pathway.
Title: Aldo-keto reductase family 1 member B8 is secreted via non-classical pathway.

data suggest that murine AKR1B8 is the ortholog of human AKR1B10.
Title: Murine aldo-keto reductase family 1 subfamily B: identification of AKR1B8 as an ortholog of human AKR1B10.

The following Akr1b8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akr1b8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01221	NM_008012.2 Latest version!	Mus musculus aldo-keto reductase family 1, member B8 (Akr1b8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OMu01221	NM_008012.1	Mus musculus aldo-keto reductase family 1, member B8 (Akr1b8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akr1b8 ( NM_008012.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032038.1 Mus musculus aldo-keto reductase family 1, member B8 (Akr1b8), mRNA.

Price & Availability↑

CloneID	OMu01221
Clone ID Related Accession (Same CDS sequence)	NM_008012.2 , NM_008012.1
Accession Version	NM_008012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	aldose reductase-related protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115767.14. On Feb 23, 2018 this sequence version replaced NM_008012.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U04204.1, BC005789.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACGT TCGTGGAACT CAGTACCAAA GCCAAGATGC CCATTGTGGG CCTGGGCACC 
TGGAAGTCTC CCCCAAACCA AGTCAAAGAA GCTGTGAAGG CGGCCATTGA CGCTGGGTAT 
CGCCATATCG ACTGCGCGTA TGCCTATTGC AACGAGAATG AGGTGGGAGA AGCCATCCAA 
GAGAAGATCA AAGAGAAGGC TGTGCAGCGG GAGGACCTCT TCATTGTCAG CAAGCTGTGG 
CCCACCTGCT TTGAGAAGAA ACTGCTAAAG GAAGCCTTTC AGAAGACCCT CACGGATCTG 
AAGCTGGACT ATTTGGACCT CTATCTTATT CACTGGCCAC AGGGACTTCA GCCAGGAAAG 
GAGTTATTCC CCAAAGATGA CCAAGGCAGA ATCCTCACCA GTAAGACAAC ATTCTTGGAA 
GCCTGGGAGG GCATGGAGGA ACTGGTGGAC CAGGGGCTGG TGAAAGCTCT GGGCGTCTCC 
AACTTCAACC ACTTCCAGAT TGAAAGGCTC CTGAACAAGC CTGGACTGAA ACATAAGCCA 
GTGACCAACC AGGTTGAGTG TCATCCTTAC CTCACCCAGG AAAAACTGAT CCAGTACTGT 
CACTCGAAGG GCATCTCTGT CACTGCCTAC AGTCCCCTGG GCTCCCCAGA CAGGCCTAGC 
GCCAAGCCAG AGGACCCTTC ACTATTAGAG GACCCCAAAA TTAAAGAGAT TGCCGCCAAG 
CACGAGAAAA CCTCAGCCCA GGTTCTGATT CGGTTTCACA TCCAGAGGAA CGTGGTGGTG 
ATCCCGAAGT CTGTGACGCC ATCACGTATA CAGGAGAACA TTCAGGTCTT TGATTTCCAG 
TTGAGTGACG AGGAGATGGC CACTATCCTT AGCTTCAACA GAAACTGGAG GGCCTGCCTG 
CTGCCTGAGA CAGTAAACAT GGAAGAATAT CCCTATGATG CAGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032038.1
CDS	86..1036
Translation	MATFVELSTKAKMPIVGLGTWKSPPNQVKEAVKAAIDAGYRHIDCAYAYCNENEVGEAIQEKIKEKAVQREDLFIVSKLWPTCFEKKLLKEAFQKTLTDLKLDYLDLYLIHWPQGLQPGKELFPKDDQGRILTSKTTFLEAWEGMEELVDQGLVKALGVSNFNHFQIERLLNKPGLKHKPVTNQVECHPYLTQEKLIQYCHSKGISVTAYSPLGSPDRPSAKPEDPSLLEDPKIKEIAAKHEKTSAQVLIRFHIQRNVVVIPKSVTPSRIQENIQVFDFQLSDEEMATILSFNRNWRACLLPETVNMEEYPYDAEY

Target ORF information:

RefSeq Version	NM_008012.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus aldo-keto reductase family 1, member B8 (Akr1b8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008012.2

ORF Insert Sequence:

ATGGCCACGT TCGTGGAACT CAGTACCAAA GCCAAGATGC CCATTGTGGG CCTGGGCACC 
TGGAAGTCTC CCCCAAACCA AGTCAAAGAA GCTGTGAAGG CGGCCATTGA CGCTGGGTAT 
CGCCATATCG ACTGCGCGTA TGCCTATTGC AACGAGAATG AGGTGGGAGA AGCCATCCAA 
GAGAAGATCA AAGAGAAGGC TGTGCAGCGG GAGGACCTCT TCATTGTCAG CAAGCTGTGG 
CCCACCTGCT TTGAGAAGAA ACTGCTAAAG GAAGCCTTTC AGAAGACCCT CACGGATCTG 
AAGCTGGACT ATTTGGACCT CTATCTTATT CACTGGCCAC AGGGACTTCA GCCAGGAAAG 
GAGTTATTCC CCAAAGATGA CCAAGGCAGA ATCCTCACCA GTAAGACAAC ATTCTTGGAA 
GCCTGGGAGG GCATGGAGGA ACTGGTGGAC CAGGGGCTGG TGAAAGCTCT GGGCGTCTCC 
AACTTCAACC ACTTCCAGAT TGAAAGGCTC CTGAACAAGC CTGGACTGAA ACATAAGCCA 
GTGACCAACC AGGTTGAGTG TCATCCTTAC CTCACCCAGG AAAAACTGAT CCAGTACTGT 
CACTCGAAGG GCATCTCTGT CACTGCCTAC AGTCCCCTGG GCTCCCCAGA CAGGCCTAGC 
GCCAAGCCAG AGGACCCTTC ACTATTAGAG GACCCCAAAA TTAAAGAGAT TGCCGCCAAG 
CACGAGAAAA CCTCAGCCCA GGTTCTGATT CGGTTTCACA TCCAGAGGAA CGTGGTGGTG 
ATCCCGAAGT CTGTGACGCC ATCACGTATA CAGGAGAACA TTCAGGTCTT TGATTTCCAG 
TTGAGTGACG AGGAGATGGC CACTATCCTT AGCTTCAACA GAAACTGGAG GGCCTGCCTG 
CTGCCTGAGA CAGTAAACAT GGAAGAATAT CCCTATGATG CAGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akr1b8 ( NM_008012.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032038.1 Mus musculus aldo-keto reductase family 1, member B8 (Akr1b8), mRNA.

Price & Availability↑

CloneID	OMu01221
Clone ID Related Accession (Same CDS sequence)	NM_008012.2 , NM_008012.1
Accession Version	NM_008012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-09
Organism	Mus musculus(house mouse)
Product	aldose reductase-related protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U04204.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U04204.1, BC005789.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACGT TCGTGGAACT CAGTACCAAA GCCAAGATGC CCATTGTGGG CCTGGGCACC 
TGGAAGTCTC CCCCAAACCA AGTCAAAGAA GCTGTGAAGG CGGCCATTGA CGCTGGGTAT 
CGCCATATCG ACTGCGCGTA TGCCTATTGC AACGAGAATG AGGTGGGAGA AGCCATCCAA 
GAGAAGATCA AAGAGAAGGC TGTGCAGCGG GAGGACCTCT TCATTGTCAG CAAGCTGTGG 
CCCACCTGCT TTGAGAAGAA ACTGCTAAAG GAAGCCTTTC AGAAGACCCT CACGGATCTG 
AAGCTGGACT ATTTGGACCT CTATCTTATT CACTGGCCAC AGGGACTTCA GCCAGGAAAG 
GAGTTATTCC CCAAAGATGA CCAAGGCAGA ATCCTCACCA GTAAGACAAC ATTCTTGGAA 
GCCTGGGAGG GCATGGAGGA ACTGGTGGAC CAGGGGCTGG TGAAAGCTCT GGGCGTCTCC 
AACTTCAACC ACTTCCAGAT TGAAAGGCTC CTGAACAAGC CTGGACTGAA ACATAAGCCA 
GTGACCAACC AGGTTGAGTG TCATCCTTAC CTCACCCAGG AAAAACTGAT CCAGTACTGT 
CACTCGAAGG GCATCTCTGT CACTGCCTAC AGTCCCCTGG GCTCCCCAGA CAGGCCTAGC 
GCCAAGCCAG AGGACCCTTC ACTATTAGAG GACCCCAAAA TTAAAGAGAT TGCCGCCAAG 
CACGAGAAAA CCTCAGCCCA GGTTCTGATT CGGTTTCACA TCCAGAGGAA CGTGGTGGTG 
ATCCCGAAGT CTGTGACGCC ATCACGTATA CAGGAGAACA TTCAGGTCTT TGATTTCCAG 
TTGAGTGACG AGGAGATGGC CACTATCCTT AGCTTCAACA GAAACTGGAG GGCCTGCCTG 
CTGCCTGAGA CAGTAAACAT GGAAGAATAT CCCTATGATG CAGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032038.1
CDS	68..1018
Translation	MATFVELSTKAKMPIVGLGTWKSPPNQVKEAVKAAIDAGYRHIDCAYAYCNENEVGEAIQEKIKEKAVQREDLFIVSKLWPTCFEKKLLKEAFQKTLTDLKLDYLDLYLIHWPQGLQPGKELFPKDDQGRILTSKTTFLEAWEGMEELVDQGLVKALGVSNFNHFQIERLLNKPGLKHKPVTNQVECHPYLTQEKLIQYCHSKGISVTAYSPLGSPDRPSAKPEDPSLLEDPKIKEIAAKHEKTSAQVLIRFHIQRNVVVIPKSVTPSRIQENIQVFDFQLSDEEMATILSFNRNWRACLLPETVNMEEYPYDAEY

Target ORF information:

RefSeq Version	NM_008012.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus aldo-keto reductase family 1, member B8 (Akr1b8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008012.1

ORF Insert Sequence:

ATGGCCACGT TCGTGGAACT CAGTACCAAA GCCAAGATGC CCATTGTGGG CCTGGGCACC 
TGGAAGTCTC CCCCAAACCA AGTCAAAGAA GCTGTGAAGG CGGCCATTGA CGCTGGGTAT 
CGCCATATCG ACTGCGCGTA TGCCTATTGC AACGAGAATG AGGTGGGAGA AGCCATCCAA 
GAGAAGATCA AAGAGAAGGC TGTGCAGCGG GAGGACCTCT TCATTGTCAG CAAGCTGTGG 
CCCACCTGCT TTGAGAAGAA ACTGCTAAAG GAAGCCTTTC AGAAGACCCT CACGGATCTG 
AAGCTGGACT ATTTGGACCT CTATCTTATT CACTGGCCAC AGGGACTTCA GCCAGGAAAG 
GAGTTATTCC CCAAAGATGA CCAAGGCAGA ATCCTCACCA GTAAGACAAC ATTCTTGGAA 
GCCTGGGAGG GCATGGAGGA ACTGGTGGAC CAGGGGCTGG TGAAAGCTCT GGGCGTCTCC 
AACTTCAACC ACTTCCAGAT TGAAAGGCTC CTGAACAAGC CTGGACTGAA ACATAAGCCA 
GTGACCAACC AGGTTGAGTG TCATCCTTAC CTCACCCAGG AAAAACTGAT CCAGTACTGT 
CACTCGAAGG GCATCTCTGT CACTGCCTAC AGTCCCCTGG GCTCCCCAGA CAGGCCTAGC 
GCCAAGCCAG AGGACCCTTC ACTATTAGAG GACCCCAAAA TTAAAGAGAT TGCCGCCAAG 
CACGAGAAAA CCTCAGCCCA GGTTCTGATT CGGTTTCACA TCCAGAGGAA CGTGGTGGTG 
ATCCCGAAGT CTGTGACGCC ATCACGTATA CAGGAGAACA TTCAGGTCTT TGATTTCCAG 
TTGAGTGACG AGGAGATGGC CACTATCCTT AGCTTCAACA GAAACTGGAG GGCCTGCCTG 
CTGCCTGAGA CAGTAAACAT GGAAGAATAT CCCTATGATG CAGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Butylated hydroxyanisole induces distinct expression patterns of Nrf2 and detoxification enzymes in the liver and small intestine of C57BL/6 mice.
Toxicology and applied pharmacology288(3)339-48(2015 Nov)
Luo L,Chen Y,Wu D,Shou J,Wang S,Ye J,Tang X,Wang XJ

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Akr1b8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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